Biophysical reviews最新文献_第3页

Fluorescence phasor analysis: basic principles and biophysical applications. 荧光相量分析：基本原理及其生物物理应用。

IF 4.9

Biophysical reviews Pub Date : 2025-03-07 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01293-y

Alvaro A Recoulat Angelini, Leonel Malacrida, F Luis González Flecha

{"title":"Fluorescence phasor analysis: basic principles and biophysical applications.","authors":"Alvaro A Recoulat Angelini, Leonel Malacrida, F Luis González Flecha","doi":"10.1007/s12551-025-01293-y","DOIUrl":"https://doi.org/10.1007/s12551-025-01293-y","url":null,"abstract":"Fluorescence is one of the most widely used techniques in biological sciences. Its exceptional sensitivity and versatility make it a tool of first choice for quantitative studies in biophysics. The concept of phasors, originally introduced by Charles Steinmetz in the late nineteenth century for analyzing alternating current circuits, has since found applications across diverse disciplines, including fluorescence spectroscopy. The main idea behind fluorescence phasors was posited by Gregorio Weber in 1981. By analyzing the complementary nature of pulse and phase fluorometry data, he shows that two magnitudes-denoted as G and S-derived from the frequency-domain fluorescence measurements correspond to the real and imaginary parts of the Fourier transform of the fluorescence intensity in the time domain. This review provides a historical perspective on how the concept of phasors originates and how it integrates into fluorescence spectroscopy. We discuss their fundamental algebraic properties, which enable intuitive model-free analysis of fluorescence data despite the complexity of the underlying phenomena. Some applications in molecular biophysics illustrate the power of this approach in studying diverse phenomena, including protein folding, protein interactions, phase transitions in lipid mixtures, and formation of high-order structures in nucleic acids.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"395-408"},"PeriodicalIF":4.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RND/HAE-1 members in the Pseudomonadota phylum: exploring multidrug resistance. 假单胞菌门的RND/HAE-1成员：探索多药耐药性。

IF 4.9

Biophysical reviews Pub Date : 2025-03-07 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01297-8

Vinnícius Machado Schelk Gomes, Ana Carolina Silva Bulla, Pedro Henrique Monteiro Torres, Manuela Leal da Silva

{"title":"RND/HAE-1 members in the Pseudomonadota phylum: exploring multidrug resistance.","authors":"Vinnícius Machado Schelk Gomes, Ana Carolina Silva Bulla, Pedro Henrique Monteiro Torres, Manuela Leal da Silva","doi":"10.1007/s12551-025-01297-8","DOIUrl":"https://doi.org/10.1007/s12551-025-01297-8","url":null,"abstract":"The hydrophobe/amphiphile efflux-1 (HAE-1) family, part of the Resistance-Nodulation-Division (RND) superfamily, plays a critical role in the development of multidrug resistance (MDR) in bacteria. Known for its broad substrate transport capacity, this family of efflux pumps can actively expel a wide range of molecules, including antibiotics, salts, and dyes, thereby reducing the intracellular concentration of toxic substances. These transporters, which form efflux systems, are primarily found in bacteria within the phylum Pseudomonadota (Proteobacteria), where they are strongly associated with increased resistance and enhanced virulence, thus contributing to bacterial survival in hostile environments. In addition, efflux systems are composed of two other protein components: Membrane Fusion Proteins (MFPs) and Outer Membrane Factors (OMFs). Notably, several bacterial species identified by the World Health Organization (WHO) as urgent priorities for new antibiotic development, such as Escherichia coli and Pseudomonas aeruginosa, have well-studied HAE-1 efflux systems, such as AcrAB-TolC and MexAB-OprM. These systems efficiently transport molecules from the periplasm to the extracellular space, facilitating bacterial persistence. In this review, we examined the current knowledge of HAE-1 efflux transporters and their roles in the physiology and survival of bacteria in the Pseudomonadota phylum.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"687-699"},"PeriodicalIF":4.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chirality transfer to nanocrystals by peptide templates and circularly polarized light. 手性转移到纳米晶体的肽模板和圆偏振光。

IF 4.9

Biophysical reviews Pub Date : 2025-03-07 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01278-x

Marcelo Yudi Icimoto, Vitor Oliveira, Iseli Lourenço Nantes

{"title":"Chirality transfer to nanocrystals by peptide templates and circularly polarized light.","authors":"Marcelo Yudi Icimoto, Vitor Oliveira, Iseli Lourenço Nantes","doi":"10.1007/s12551-025-01278-x","DOIUrl":"https://doi.org/10.1007/s12551-025-01278-x","url":null,"abstract":"Since the early advent of nanotechnology, proteins, peptides, and amino acids have frequently been used to synthesize and stabilize metallic and ceramic nanoparticles. Also, several signaling peptides and enzymes have the activity modulated by the association with nanostructured particles and films. Lately, with the discovery of giant magnetoresistance and chiral-induced spin selectivity, an innovative nanotechnological use of amino acids and proteins emerged. Enantiomeric pairs of amino acids, peptides, and other biomolecules have been used as templates for growing chiral distorted nanocrystals and for chiral functionalization of achiral nanoparticles. More recently, circularly polarized light has been raised as an alternative for synthesizing enantiomeric pairs of plasmonic nanocrystals on anisotropic seeds. These chiral nanostructured materials exhibit unique properties with applications in biological and technological fields harnessed in various applications, including biosensing, asymmetric catalysis, and optical devices. This review presents the experimental strategies and mechanisms of chirality transfer to plasmonic and ceramic nanoparticles using peptide templates and circularly polarized light.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"409-417"},"PeriodicalIF":4.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical communication pathways in bacteria: an extra layer to quorum sensing. 细菌的物理通信途径：群体感应的额外层。

IF 4.9

Biophysical reviews Pub Date : 2025-03-04 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01290-1

Virgilio de la Viuda, Javier Buceta, Iago Grobas

引用次数: 0

Structural determinants of soft memory in recurrent biological networks. 循环生物网络中软记忆的结构决定因素。

IF 4.9

Biophysical reviews Pub Date : 2025-03-03 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01295-w

Maria Sol Vidal-Saez, Jordi Garcia-Ojalvo

引用次数: 0

Magnesium sulfate in oxidative stress-associated pathologies: clinical, cellular, and molecular perspectives. 硫酸镁在氧化应激相关病理：临床，细胞和分子的观点。

IF 4.9

Biophysical reviews Pub Date : 2025-03-01 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01292-z

Reinaldo Marín, Cilia Abad, Deliana Rojas, Miguel Fernández, Fernando Ruette

{"title":"Magnesium sulfate in oxidative stress-associated pathologies: clinical, cellular, and molecular perspectives.","authors":"Reinaldo Marín, Cilia Abad, Deliana Rojas, Miguel Fernández, Fernando Ruette","doi":"10.1007/s12551-025-01292-z","DOIUrl":"https://doi.org/10.1007/s12551-025-01292-z","url":null,"abstract":"Magnesium sulfate (MgSO₄) is a therapeutically versatile agent used across various medical conditions. This review integrates experimental and computational findings to elucidate the clinical, cellular, molecular, and electronic mechanisms underlying MgSO₄'s therapeutic effects, focusing on its antioxidant properties. MgSO₄ remains the gold standard treatment for preeclampsia and eclampsia, preventing seizures and mitigating oxidative damage. In preterm birth, it offers fetal neuroprotection, although its efficacy as a tocolytic agent is limited. MgSO₄ also shows promise in treating respiratory conditions, notably severe asthma, where it acts as a bronchodilator. Its applications extend to anesthesia, pain management, and cardiac arrhythmias, reflecting its diverse pharmacological actions. Advanced computational methods, including molecular dynamics simulations and quantum chemistry calculations, have revealed how MgSO₄ interacts with cell membranes and neutralizes hydroxyl radicals. These studies suggest that MgSO₄'s antioxidant effects stem from its ability to stabilize membrane structures and modulate electron transfer processes. The therapeutic effects are mediated through multiple pathways, including calcium channel modulation, NMDA receptor antagonism, and anti-inflammatory mechanisms. Although generally safe, MgSO₄ requires careful monitoring due to its narrow therapeutic window. Future research should focus on precision dosing strategies, innovative delivery systems, and expanded therapeutic applications. A comprehensive understanding of MgSO₄'s molecular mechanisms and clinical applications will further optimize its therapeutic use.Graphical abstract: ","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"511-535"},"PeriodicalIF":4.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical Reviews: welcoming a new year in biophysics. 生物物理评论：迎接新的一年的生物物理学。

IF 4.9

Biophysical reviews Pub Date : 2025-02-27 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-025-01291-0

Wilma K Olson

引用次数: 0

Hydrogen peroxide transport by aquaporins: insights from molecular modeling and simulations. 过氧化氢通过水通道蛋白运输：从分子模型和模拟的见解。

IF 4.9

Biophysical reviews Pub Date : 2025-02-20 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01288-9

Jonathan Chevriau, Gerardo Zerbetto De Palma, Karina Alleva, Ari Zeida

{"title":"Hydrogen peroxide transport by aquaporins: insights from molecular modeling and simulations.","authors":"Jonathan Chevriau, Gerardo Zerbetto De Palma, Karina Alleva, Ari Zeida","doi":"10.1007/s12551-025-01288-9","DOIUrl":"https://doi.org/10.1007/s12551-025-01288-9","url":null,"abstract":"Hydrogen peroxide (H2O2) is a key reactive oxygen species involved in cellular redox signaling and oxidative stress. Due to its polar nature, its transport across membranes is regulated by aquaporins (AQPs), membrane channels traditionally known for H2O transport. Certain AQPs, known as peroxiporins, facilitate selective H2O2 permeation, playing critical roles in mantaining redox homeostasis. This review summarizes insights from molecular dynamics (MD) simulations into the mechanisms of H2O2 transport through AQPs. Key structural regions, such as the selectivity filter (SF) and NPA motif, influence H2O2 permeation, with energy profiles revealing differences from H2O transport. While molecular mimicry suggests similarities in the movement of H2O and H2O2, specific interactions and energetic barriers highlight the complexity of the process. We highlight the need for integrating computational and experimental findings for further studies to unify mechanistic understanding and develop applications in redox biology.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"301-308"},"PeriodicalIF":4.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal hexacyanoferrates in photodynamic and photothermal therapies. 金属六氰高铁在光动力和光热疗法中的应用。

IF 4.9

Biophysical reviews Pub Date : 2025-02-20 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01287-w

Patrícia Alves de Matos, Hellen Cristina Novais de Oliveira, Murillo Néia Thomaz da Silva, Edson Nossol, Tayana Mazin Tsubone

{"title":"Metal hexacyanoferrates in photodynamic and photothermal therapies.","authors":"Patrícia Alves de Matos, Hellen Cristina Novais de Oliveira, Murillo Néia Thomaz da Silva, Edson Nossol, Tayana Mazin Tsubone","doi":"10.1007/s12551-025-01287-w","DOIUrl":"https://doi.org/10.1007/s12551-025-01287-w","url":null,"abstract":"Photodynamic therapy (PDT) involves a reaction between photosensitizers (PS) and oxygen (O2) to generate cytotoxic reactive oxygen species (ROS), which effectively eliminate undesired cells. Compared to conventional treatments like surgery, radiation, and chemotherapy, PDT offers several advantages, including minimal toxicity to healthy tissues and no long-term systemic side effects. However, its therapeutic efficacy is limited under hypoxic conditions, as the process relies on the presence of oxygen in the target tissue. To address these challenges, combining PDT with photothermal therapy (PTT) creates a synergistic phototherapy approach. The heat generated by PTT enhances blood flow in tumors, increasing oxygen delivery to tumor sites and boosting PDT's effectiveness. These combinations are being explored in PDT/PTT as an innovative, synergistic cancer treatment strategy, aiming to enhance the therapeutic index. One promising strategy to connect both PDT and PTT therapies involves developing nanosystems that integrate metal hexacyanoferrate (MHCF) nanoparticles with multifunctional PS. Here, we review several studies that have evaluated the combination of MHCF with various PSs to apply PDT and PTT synergistically. We discuss how nanocomposites based on these materials can address the challenges and limitations still faced in PDT/PTT. This review aims to identify new opportunities for the application of metal hexacyanoferrates in these phototherapeutic modalities.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"561-577"},"PeriodicalIF":4.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The brightness of lectins conjugated to quantum dots. 与量子点结合的凝集素的亮度。

IF 4.9

Biophysical reviews Pub Date : 2025-02-13 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01283-0

João V A Lima, Weslley F Oliveira, Abdênego R Silva, Francisco P T Melo, Martha S Ribeiro, Paulo E Cabral Filho, Adriana Fontes

{"title":"The brightness of lectins conjugated to quantum dots.","authors":"João V A Lima, Weslley F Oliveira, Abdênego R Silva, Francisco P T Melo, Martha S Ribeiro, Paulo E Cabral Filho, Adriana Fontes","doi":"10.1007/s12551-025-01283-0","DOIUrl":"https://doi.org/10.1007/s12551-025-01283-0","url":null,"abstract":"One of the main focuses of glycobiology is investigating the synthesis and modification of carbohydrates in biological systems, due to their involvement in various processes such as cell recognition, differentiation, and immune response. Since the study of these glycans contributes to the understanding of complex biological functions, these biochemical compounds can be analyzed using lectins, which are ubiquitous proteins in nature capable of specifically recognizing carbohydrates. In addition, lectin-carbohydrate interaction can be visualized by conjugating these proteins with quantum dots (QDs), which are fluorescent nanoprobes with advantageous properties, including photostability and size-tunable emission. QDs also possess chemically active surfaces that enable the attachment of biomolecules, such as lectins. In this review, we provide detailed reports of studies involving QD-lectin conjugates conducted by the Biomedical Nanotechnology Group at the Federal University of Pernambuco (UFPE/Brazil) and its collaborators. An integrated perspective on the use of QD-lectin conjugates to study saccharides in a range of biological systems, from bacteria and fungi to red blood cells and cancer tissues, is also presented. We hope this comprehensive review inspires further studies exploring the brightness of lectins upon conjugation with QDs to unravel glycobiological processes.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"419-434"},"PeriodicalIF":4.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0