Biophysical reviews最新文献_第6页

Editorial to the topical issue: the 7th Nanoengineering for Mechanobiology Symposium 2024 Camogli, Genoa, Italy. 时事社论：第七届机械生物学纳米工程研讨会2024卡莫格利，热那亚，意大利。

IF 4.9

Biophysical reviews Pub Date : 2024-12-13 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01262-x

Costanza Giampietro, Aldo Ferrari, Massimo Vassalli

引用次数: 0

Effect of low doses of gamma radiation on cell growth over an electrospun polycaprolactone/zinc acetate scaffold for biomedical applications. 低剂量伽马辐射对生物医学用静电纺聚己内酯/醋酸锌支架上细胞生长的影响。

IF 4.9

Biophysical reviews Pub Date : 2024-12-09 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01256-9

Naglaa M Ismail, Soheir Korraa, M B S Osman, Eman El Sheikh

{"title":"Effect of low doses of gamma radiation on cell growth over an electrospun polycaprolactone/zinc acetate scaffold for biomedical applications.","authors":"Naglaa M Ismail, Soheir Korraa, M B S Osman, Eman El Sheikh","doi":"10.1007/s12551-024-01256-9","DOIUrl":"10.1007/s12551-024-01256-9","url":null,"abstract":"The objective of the present study was to generate functional biomaterials to repair and re-establish damaged tissues by producing porous biopolymeric PCL/zinc acetate scaffolds using the electrospinning technique and studying the effect of low doses of gamma radiation on cell proliferation. In electrospinning, ultrafine fibers are spun in a high-voltage electrostatic field. The electrospun structure has natural tissue morphology, which is distinguished by high porosity, a broad variety of pore diameters, efficient mechanical qualities, and the ability to promote cell proliferation and adhesion. PCL/zinc acetate scaffold was investigated by scanning electron microscope (SEM) techniques, Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). Then, they were sterilized by ionizing radiation (gamma radiation) with a dose of 30 KGy for the cell culture part. Scaffold biocompatibility tests were carried out by using Vero cells. Cells grown on scaffolds were irradiated with doses of 0.5, 1, 2.5, and 5 Gy gamma radiation. Cell viability was examined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, SEM, malondialdehyde (MDA), and nitric oxide (NO) content. The results proved that cell viability was increased after γ-irradiation with 0.5 Gy compared to control (without γ-irradiation), then decreased with other doses (1, 2.5, and 5 Gy), while the dose of 5 Gy caused the least cell viability. Finally, it was concluded that the nanofiber PCL/zinc acetate scaffold could be successfully fabricated by using the electrospinning technique, and it was biocompatible with Vero cells.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"139-149"},"PeriodicalIF":4.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid-liquid phase separation of Tau and α-synuclein: a new target for treating comorbidity in neurodegeneration. 液液相分离Tau蛋白和α-突触核蛋白：治疗神经退行性疾病的新靶点。

IF 4.9

Biophysical reviews Pub Date : 2024-12-02 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-024-01259-6

Nahuel N Foressi, Leandro Cruz Rodríguez, M Soledad Celej

{"title":"Liquid-liquid phase separation of Tau and α-synuclein: a new target for treating comorbidity in neurodegeneration.","authors":"Nahuel N Foressi, Leandro Cruz Rodríguez, M Soledad Celej","doi":"10.1007/s12551-024-01259-6","DOIUrl":"https://doi.org/10.1007/s12551-024-01259-6","url":null,"abstract":"Alzheimer's and Parkinson's diseases are the most common neurodegenerative disorders, causing significant disability and mortality worldwide. Though traditionally classified as Tau and α-synuclein-related disorders, respectively, there is growing evidence of clinical overlap between dementia and Parkinsonism, with comorbidity worsening cognitive impairment and prognosis. Emerging research on liquid-liquid phase separation (LLPS) offers promising insights into novel treatments of these proteinopathies by targeting the phase behavior of the disease-associated proteins. Thus, manipulating condensates has become a focus for developing new therapeutic compounds, termed condensate-modifying drugs (c-mods), by which historically considered undruggable proteins can be targeted. This review offers an overview of bioactive molecules that act as modifiers of Tau and α-synuclein condensates through various mechanisms. The goal is to lay the groundwork for discovering new therapeutic approaches to prevent harmful protein aggregation and treat comorbidity in tau and synucleinopathies.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 2","pages":"491-498"},"PeriodicalIF":4.9,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Dimensional reduction and adaptation-development-evolution relation in evolved biological systems. 修正：进化生物系统中的降维与适应-发展-进化关系。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01255-w

Kunihiko Kaneko

引用次数: 0

Tracing the birth and intrinsic disorder of loops and domains in protein evolution. 追踪蛋白质进化中环和结构域的诞生和内在紊乱。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01251-0

Gustavo Caetano-Anollés, Fizza Mughal, M Fayez Aziz, Kelsey Caetano-Anollés

{"title":"Tracing the birth and intrinsic disorder of loops and domains in protein evolution.","authors":"Gustavo Caetano-Anollés, Fizza Mughal, M Fayez Aziz, Kelsey Caetano-Anollés","doi":"10.1007/s12551-024-01251-0","DOIUrl":"10.1007/s12551-024-01251-0","url":null,"abstract":"Protein loops and structural domains are building blocks of molecular structure. They hold evolutionary memory and are largely responsible for the many functions and processes that drive the living world. Here, we briefly review two decades of phylogenomic data-driven research focusing on the emergence and evolution of these elemental architects of protein structure. Phylogenetic trees of domains reconstructed from the proteomes of organisms belonging to all three superkingdoms and viruses were used to build chronological timelines describing the origin of each domain and its embedded loops at different levels of structural abstraction. These timelines consistently recovered six distinct evolutionary phases and a most parsimonious evolutionary progression of cellular life. The timelines also traced the birth of domain structures from loops, which allowed to model their growth ab initio with AlphaFold2. Accretion decreased the disorder of the growing molecules, suggesting disorder is molecular size-dependent. A phylogenomic survey of disorder revealed that loops and domains evolved differently. Loops were highly disordered, disorder increased early in evolution, and ordered and moderate disordered structures were derived. Gradual replacement of loops with α-helix and β-strand bracing structures over time paved the way for the dominance of more disordered loop types. In contrast, ancient domains were ordered, with disorder evolving as a benefit acquired later in evolution. These evolutionary patterns explain inverse correlations between disorder and sequence length of loops and domains. Our findings provide a deep evolutionary view of the link between structure, disorder, flexibility, and function.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 6","pages":"723-735"},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing aptamer structure and interactions: in silico modelling and instrumental methods. 分析适体结构和相互作用：在硅建模和仪器方法。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01252-z

Daria O Malysheva, Maya A Dymova, Vladimir A Richter

{"title":"Analyzing aptamer structure and interactions: in silico modelling and instrumental methods.","authors":"Daria O Malysheva, Maya A Dymova, Vladimir A Richter","doi":"10.1007/s12551-024-01252-z","DOIUrl":"10.1007/s12551-024-01252-z","url":null,"abstract":"Aptamers are short oligonucleotides that bind specifically to various ligands and are characterized by their low immunogenicity, thermostability, and ease of labeling. Many biomedical applications of aptamers as biosensors and drug delivery agents are currently being actively researched. Selective affinity selection with exponential ligand enrichment (SELEX) allows to discover aptamers for a specific target, but it only provides information about the sequence of aptamers; hence other approaches are used for determining aptamer structure, aptamer-ligand interactions and the mechanism of action. The first one is in silico modelling that allows to infer likely secondary and tertiary structures and model their interactions with a ligand. The second approach is to use instrumental methods to study structure and aptamer-ligand interaction. In silico modelling and instrumental methods are complimentary and their combined use allows to eliminate some ambiguity in their respective results. This review examines both the advantages and limitations of in silico modelling and instrumental approaches currently used to study aptamers, which will allow researchers to develop optimal study designs for analyzing aptamer structure and ligand interactions.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 6","pages":"685-700"},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of the Aβ-peptide structural diversity: molecular dynamics approaches. a - β肽结构多样性的研究：分子动力学方法。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01253-y

Anna P Tolstova, Alexei A Adzhubei, Maria A Strelkova, Alexander A Makarov, Vladimir A Mitkevich

引用次数: 0

Diatoms: harnessing nature's microscopic marvels for biosensing and multifaceted applications. 硅藻：利用自然界的微观奇迹进行生物传感和多方面的应用。

IF 4.9

Biophysical reviews Pub Date : 2024-11-05 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01249-8

Krupa Bhat, Maryam Abdul Ajees, Pawas Kumar, Vibha, Vyasraj G Bhat, Roopa Nayak, Nirmal Mazumder

{"title":"Diatoms: harnessing nature's microscopic marvels for biosensing and multifaceted applications.","authors":"Krupa Bhat, Maryam Abdul Ajees, Pawas Kumar, Vibha, Vyasraj G Bhat, Roopa Nayak, Nirmal Mazumder","doi":"10.1007/s12551-024-01249-8","DOIUrl":"10.1007/s12551-024-01249-8","url":null,"abstract":"This article discusses the use of diatom in biosensing and various applications. A thorough understanding of the biosensing properties of diatoms is essential for the advancement of life sciences technologies. This review elucidates the emerging significance of diatoms in biosensing applications by highlighting the high surface area-to-volume ratio, biocompatibility, and facile functionalization of them. We examined the possible application of diatoms as flexible biosensing systems for the detection of various analytes, such as biological molecules, heavy metals, and poisons, by considering the findings of earlier research. Additionally, to show how diatoms can be used to create precise and sensitive biosensors, by integrating with several transduction modalities, including optical, electrochemical, and piezoelectric methods. We also delve into the potential applications of diatom-based biosensing in the future as well as pertinent topics such as repeatability, stability, and scalability. This provides an in-depth analysis of the quickly evolving field of diatom-based biosensing, which could affect several industries, such as environmental monitoring, food security and medical diagnostics.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"103-125"},"PeriodicalIF":4.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dry lab microscopist or prompt microscopist: do we need them? 干式实验室显微镜师或即时显微镜师：我们需要他们吗？

IF 4.9

Biophysical reviews Pub Date : 2024-10-30 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01250-1

Filip Braet, Weidong Cai

引用次数: 0

Structure, function, and biophysics of bacterial motility and the flagellar motor-IUPAB2024 session commentary. 细菌运动和鞭毛运动的结构，功能和生物物理学- iupab2024会议评论。

IF 4.9

Biophysical reviews Pub Date : 2024-10-28 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01243-0

Matthew A B Baker, Seiji Kojima

引用次数: 0