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Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens. 超滤纯化传染性法氏囊病病毒对特定无病原体鸡免疫反应和细胞因子活化的影响。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2009350.3978
Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili
{"title":"Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens.","authors":"Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili","doi":"10.30466/vrf.2023.2009350.3978","DOIUrl":"10.30466/vrf.2023.2009350.3978","url":null,"abstract":"<p><p>Purification is an important step in the production of viral vaccines that strongly affects product recovery and subsequent immune responses. The present study was carried out with the aim of improving the purification of infectious bursal disease virus (IBDV) by the tangential flow filtration (TFF) method. Then, the effect of the purified virus on the induction of immune responses against IBDV in specific pathogen free (SPF) chickens was investigated. The IBD07IR strain was propagated in embryonated SPF eggs. The virus was purified using a 100 kDa cassette. The quality of the recovered viruses was evaluated by titration. A total number of 60 SPF chickens were randomly divided into three groups (n = 20) and received the concentrated viral antigen, commercial live IBDV vaccine and phosphate-buffered saline at the age of 3 weeks by eye drop method. The bursa of Fabricius was examined histopathologically for possible changes. Sera were collected at 1-week intervals from day 0 until the end of 6 weeks after vaccination. The IBDV-specific antibody levels, induction of cell-mediated immunity and mRNA expression levels of cytokines were evaluated. The results showed that despite a relative raise in virus titer from 7.66 to 8.17 embryo infectious dose (EID)<sub>50</sub> mL<sup>-1</sup> following purification, both the purified IBDV and commercial vaccine are able to induce strong immune responses against the virus. Within a context of egg-based IBDV vaccine production, a single-step TFF can be applied for the relatively purification. This platform requires a further study in the selection of multiple membranes to optimize the operating conditions and final product.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell proliferation and apoptosis in canine oral papillomatosis. 犬口腔乳头状瘤病的细胞增殖和凋亡。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.1996086.3818
Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz
{"title":"Cell proliferation and apoptosis in canine oral papillomatosis.","authors":"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz","doi":"10.30466/vrf.2023.1996086.3818","DOIUrl":"10.30466/vrf.2023.1996086.3818","url":null,"abstract":"<p><p>This study was aimed at the evaluation of cell proliferation, <i>p53</i> level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant <i>p53</i> gene. Despite the mutation of <i>p53</i> gene, the shift in the <i>Bax</i>/<i>Bcl-2</i> ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic <i>Bax</i> gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow. 揭示奶牛子宫纵隔肌瘤声像图和组织病理学的综合探索。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2018975.4112
Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar
{"title":"Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow.","authors":"Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar","doi":"10.30466/vrf.2024.2018975.4112","DOIUrl":"10.30466/vrf.2024.2018975.4112","url":null,"abstract":"<p><p>Genital tumours are rare among cattle, largely due to their relatively short lifespans. Leio-myoma, a smooth muscle tumour being more prevalent in dogs, appears only at a rate of 1.00 - 2.00% in cattle, affecting reproductive efficiency in cases of complete uterine obstruction. This case report involves an 8-year-old cow with repeated insemination attempts unveiled 5.00 cm intra-luminal uterine mass, obstructing the right uterine horn. Transrectal sonography (TRUS) revealed a highly vascularized mass with normal ovarian function. Confirmation of clinical condition, <i>i.e.</i>, uterine leiomyoma, <i>via</i> uterine biopsy concluded the presence of neoplastic smooth muscle cells arranged in interlacing bundles showing mild pleomorphism, and special staining using Masson's trichrome revealed an unappreciable amount of connective tissue; subsequently right flank celiotomy was performed to remove the benign tumour. Forty-five days after celiotomy, TRUS examination confirmed an unobstructed uterine horn, and bilateral oviduct patency was adjudged with 2.50% methylene blue. Following treatment for chronic endometritis, artificial insemination led to conception nearly 90 days post-procedure. The TRUS aids preliminary diagnosis, while definitive identification demands necropsy and surgical methods. This case underscores the diagnostic significance of TRUS, histopathology and celiotomy for identifying and managing uterine leiomyoma in cattle.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam. 氯胺酮、异丙酚和异氟烷对使用美托咪啶和咪达唑仑进行预处理的临床健康犬心电图变量的影响。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2008055.3954
{"title":"Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam.","authors":"","doi":"10.30466/vrf.2024.2008055.3954","DOIUrl":"10.30466/vrf.2024.2008055.3954","url":null,"abstract":"<p><p>The purpose of this study was to investigate the effects of three anesthetic agents, with premedication of medetomidine and midazolam, on electrocardiographic variables in dogs. Ten adult mixed breed dogs were used in a crossover design study, where they received ketamine, propofol and isoflurane treatments with a one-week washout period between them. In all three groups, medetomidine was administered first followed by midazolam after 15 min. Then, after 20 min, group 1 received ketamine intravenously (IV), group 2 received propofol (IV), and group 3 received isoflurane (inhalation). In all dogs, electrocardiographs were taken before and after premedication's, as well as every 15 min during anesthesia. Medetomidine significantly decreased heart rate and P wave amplitude and increased PR interval, R wave amplitude, QT interval, and T wave amplitude. Midazolam increased the amplitude of the R and T waves. Ketamine increased the heart rate and PR interval. Propofol increased the heart rate for up to 15 min, decreased the PR interval for up to 30 min, and the QT interval for up to 45 min. Isoflurane increased the heart rate and decreased the amplitude of R and T waves. The results showed that the drugs used in this study did not have many side effects on electrocardiographic variables and could be used without serious concern. The most important side effects observed were a severe reduction in heart rate and 1<sup>st</sup> degree atrioventricular (AV) block and, to a lesser extent, 2<sup>nd</sup> degree AV block caused by medetomidine and midazolam which were masked by the anesthetics.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test. 根据粪便幼虫数量减少试验评估阿苯达唑和左旋咪唑对羊群肺线虫的抗药性
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2023.2010062.3991
Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin
{"title":"Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test.","authors":"Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin","doi":"10.30466/vrf.2023.2010062.3991","DOIUrl":"10.30466/vrf.2023.2010062.3991","url":null,"abstract":"<p><p>The over-use of anti-parasitic compounds as a method of control has led to insufficient effectiveness and widespread drug resistance worldwide. The aim of this study was to investigate the efficacy of albendazole and levamisole as anti-parasitic agents in a lung worm control program in goat flocks. During 2021 and 2022, a total of 110 goats (age of four months and above) were randomly selected from 11 herds in the north-western region of Iran including Saanen breed (both sexes of the same age). The results indicated that 3.60, 50.80 and 41.90% were respectively infected with <i>Dictyocaulus filaria</i>, <i>Muellerius capillaris</i> and <i>Proto-strongylus rufescens</i>, and generally all the lung parasites in goats of this region were resistant to albendazole and levamisole. Due to clinical importance of <i>D. filaria</i> in goats, the molecular analysis of two samples was also done. Sequencing results showed that the identified parasites were 100% similar to the reference sequences registered in the GenBank<sup>®</sup>. The results of this research showed low level of these anthelmintics efficacy against <i>Dictyocaulus</i> and <i>Muellerius</i>. Generally, the lung parasites in goats of this region are resistant to albendazole and levamisole. The <i>P. rufescens</i> showed high resistance to these drugs. Totally, it can be concluded that the level of drug resistance varies in different parts of the world; but, the frequencies of drug resistance in different parts of the world are not the same, requiring more studies.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Follicular, luteal dynamics and effect of synchronization methods on reproductive responses in Cholistani cows. 乔里斯坦奶牛的卵泡和黄体动态以及同步方法对繁殖反应的影响。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2024.2004618.3913
Muhammad Awais Ajmal, Mubbashar Hassan, Muhammad Shahzad, Muhammad Ilyas Naveed, Aijaz Ali Channa, Nasim Ahmad
{"title":"Follicular, luteal dynamics and effect of synchronization methods on reproductive responses in Cholistani cows.","authors":"Muhammad Awais Ajmal, Mubbashar Hassan, Muhammad Shahzad, Muhammad Ilyas Naveed, Aijaz Ali Channa, Nasim Ahmad","doi":"10.30466/vrf.2024.2004618.3913","DOIUrl":"10.30466/vrf.2024.2004618.3913","url":null,"abstract":"<p><p>The Cholistani cow is considered one of the milch breeds of Zebu cattle. Despite being heat and tick-resistant, it has not yet gained much popularity among Pakistan dairy farmers due to its long calving interval. This study aimed to understand the basic reproductive physiology of Cholistani cows using sonography and reproductive biotechnologies such as estrus synchronization and timed artificial insemination to improve reproductive efficiency. In experiment 1, six Cholistani cows with mixed parity 3.20 ± 1.30 and weighing 400 kg were selected to monitor ovarian dynamics on alternate days by the same sonographer from the onset of heat through ovulation until the next ovulation. Experiment 2 measured the effect of estrus synchronization methods, controlled internal drug release-gonadotropin-releasing hormone (CIDR-GnRH, n = 31) and Ovsynch (OVS, n = 32) on various reproductive parameters. The mean estrous cycle length was 19.81 ± 0.56 days with two follicular waves. The mean inter-ovulatory interval was 20.80 ± 0.52 days, with a preovulatory follicular size of 13.83 ± 2.37 mm. Estrus response was higher (<i>p</i> > 0.05) in controlled internal drug release (CIDR)-GnRH (93.54%) than in OVS (84.37%) cows. Similarly, ovulation and conception rates were higher in CIDR-GnRH (91.66% <i>vs</i>. 68.42%) than in OVS cows (41.37% <i>vs.</i> 33.33%), respectively. In conclusion, CIDR-GnRH results in a better estrus response, higher ovulation rate, and subsequently greater conception rate than OVS in <i>Bos indicus</i> dairy cows.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tonsillar squamous cell carcinoma in a male Lhasa Apso. 一只雄性拉萨阿普索犬的扁桃体鳞状细胞癌。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2017462.4096
Alaleh Rakhshanpour, Sayyed Jafar Hasani, Ali-Asghar Tehrani, Mehdi Behfar
{"title":"Tonsillar squamous cell carcinoma in a male Lhasa Apso.","authors":"Alaleh Rakhshanpour, Sayyed Jafar Hasani, Ali-Asghar Tehrani, Mehdi Behfar","doi":"10.30466/vrf.2024.2017462.4096","DOIUrl":"https://doi.org/10.30466/vrf.2024.2017462.4096","url":null,"abstract":"<p><p>Squamous cell carcinoma (SCC) and more specifically tonsillar SCC are generally regarded as locally invasive with various rates of metastasis. A 13-year-old male Lhasa Apso was referred to the clinic with an unusual mass on his left tonsil and abnormal lumps under the tongue. The dog's food and water intake, breathing and heart rates, body temperature, capillary refill time, and blood tests were normal. A biopsy of abnormal tissue and immunohistochemical analysis were performed. Histopathological and immunohistochemical evaluations confirmed the basaloid SCC. Some treatments like surgery, radiotherapy, and chemotherapy are used to treat a specific tumor or area of the body. However, in this particular case, chemotherapy was not administered. Considering that dogs with tonsillar cancer generally have a poor to guarded prognosis and a short life expectancy, this case was euthanized after one year with the owner's consent due to the significant tumor enlargement.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An outbreak of Akabane disease in a cattle herd on the Mughan plain, Iran. 伊朗穆汉平原上的牛群爆发赤痢病。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2024.2012333.4021
Yousef Davoudi, Mohammad Nouri, Mohammad Rahim Haji Hajikolaei, Shobeir Yazdani Paraei, Amir Javadi, Saleh Esmaeilzadeh
{"title":"An outbreak of Akabane disease in a cattle herd on the Mughan plain, Iran.","authors":"Yousef Davoudi, Mohammad Nouri, Mohammad Rahim Haji Hajikolaei, Shobeir Yazdani Paraei, Amir Javadi, Saleh Esmaeilzadeh","doi":"10.30466/vrf.2024.2012333.4021","DOIUrl":"10.30466/vrf.2024.2012333.4021","url":null,"abstract":"<p><p>In November 2021, an investigation was conducted into an outbreak of abortion, stillbirth, and the birth of calves with congenital abnormalities (arthrogryposis and hydranencephaly) at a dairy farm in Dasht-e-Mughan city, Ardabil province<b>.</b> A total of 70 cows experienced these issues. To determine the cause of the outbreak, post-mortem brain tissue samples were collected from two calves affected by hydranencephaly, which occurred shortly after their birth. Polymerase chain reaction (PCR) testing was conducted for multiple viruses, including bovine viral diarrhea (BVD), border disease, Akabane, Schmallenberg, and bluetongue viruses (BTVs). The samples were positive only for Akabane virus. Serum samples were collected from a group of 60 cattle, consisting of 45 adult cows and 15 younger calves aged between 8 to 10 months. These samples were analyzed to detect the presence of antibodies against the Akabane and Schmallenberg viruses. Both of these viruses are known to be responsible for causing abortion, stillbirth, and congenital abnormalities in calves. Among 45 cows that tested by competitive enzyme-linked immunosorbent assay (cELISA), 26.66% and 33.33% exhibited antibodies against Akabane and Schmallenberg viruses, respectively. Notably, 20.00% of cows co-exhibited antibodies for both viruses. Despite PCR evidence implicating Akabane virus as the principal etiology of clinical signs observed in the affected herd, the high co-seropositivity to Schmallenberg virus, warrants a thorough investigation into potential viral interactions. Further research is required to determine the source of the virus and their transmission routes. This information could facilitate the refinement of disease control strategies and improving the management of reproductive challenges in such affected herds.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a multiplexed Luminex assay for simultaneous detection of enteric viruses in cattle. 开发用于同时检测牛肠道病毒的多重 Luminex 检测法。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2023-01-15 DOI: 10.30466/vrf.2023.2005728.3925
Monika Punia, Sushila Maan, Kanisht Batra, Aman Kumar, Narender Singh Maan, Suresh Kumar Gahlawat
{"title":"Development of a multiplexed Luminex assay for simultaneous detection of enteric viruses in cattle.","authors":"Monika Punia, Sushila Maan, Kanisht Batra, Aman Kumar, Narender Singh Maan, Suresh Kumar Gahlawat","doi":"10.30466/vrf.2023.2005728.3925","DOIUrl":"10.30466/vrf.2023.2005728.3925","url":null,"abstract":"<p><p>Viral and bacterial gastroenteritis and diarrhea have long been a problem in livestock with devastating effects on animal health and production causing a heavy financial burden on producers. Therefore, the bead-based multiplex detection assay was created for simultaneous detection of three livestock viral diarrheic agents <i>viz</i>. bovine rotavirus (BRV), bovine coronavirus (BCoV) and bluetongue virus (BTV). The primers and probes for triplex MAGPIX assay for simultaneous detection of three enteric viruses were designed and the assay was optimized for hybridization temperature, primer-probe and bead concentrations. The newly developed MAGPIX assay was used to determine the prevalence of these diarrhea-associated viruses by testing 200 fecal samples collected from Haryana state of India during 2018-2019. The limit of detection of the developed triplex assay was 1 × 10<sup>5</sup>, 1 × 10<sup>4</sup>, and 1 × 10<sup>5</sup> RNA copies for BRV, BCoV, and BTV, respectively, being lower than the reverse transcription-quantitative polymerase chain reaction (RT-qPCR). However, it was higher than the conventional RT-PCR, showing it to be more sensitive. The newly developed MAGPIX assay was a rapid, cost-effective and high throughput diagnostic tool for identification of three major entero-pathogenic diarrhea associated viruses, either alone or in tandem, with the aim to prevent and control viral diarrhea in animals.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Radiographic assessment of normal parameters of forelimb distal phalanx in Turkmen horses. 土库曼马前肢远端趾骨正常参数的放射学评估。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.562962.3653
Keramat Allah Khaledi, Ali Mirshahi, Kamran Sardari, Mohammad Azizzadeh, Samaneh Ghasemi
{"title":"Radiographic assessment of normal parameters of forelimb distal phalanx in Turkmen horses.","authors":"Keramat Allah Khaledi, Ali Mirshahi, Kamran Sardari, Mohammad Azizzadeh, Samaneh Ghasemi","doi":"10.30466/vrf.2023.562962.3653","DOIUrl":"10.30466/vrf.2023.562962.3653","url":null,"abstract":"<p><p>The Turkmen horse is one of the oldest breeds in the world with unique characteristics in agility, endurance and jump, being publicly acclaimed. Laminitis affects the limbs and decreases athletic performance. Radiographic evaluation is essential to confirm the sinking diagnosis in every breed of a horse suffering from laminitis. Since no information about distal phalanx radiographic measurements in this breed has been reported, the current study was designed and conducted. In the present study, 24 clinically sound registered Turkmen horses of both sexes (15 mares and nine stallions) were selected. Lateromedial radiographic views of both front distal phalanges were taken, focusing on the distal phalanx. After magnification correction, images were used to measure founder distances (FD) and other distal phalanx radiographic measurements using an image processing program. There was no significant difference between radiographic measurements of the left and right front feet. The FD had a significant positive correlation with weight. The horses under 3 years of age had a significantly greater U angle than the horses over the age of three. Also, palmarocortical length (PCL) in the Turkmen stallions was significantly higher than mares. In this investigation, PCL in horses under the age of three was significantly lower than horses over 3 years of age. The values determined in this study can be used as baseline data of front feet in Turkmen horses.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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