Veterinary Research Forum最新文献_第5页

Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran. 伊朗养蜂场欧洲臭蝇病主、次病原检测的流行病学研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2026240.4232

Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani

{"title":"Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran.","authors":"Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani","doi":"10.30466/vrf.2024.2026240.4232","DOIUrl":"https://doi.org/10.30466/vrf.2024.2026240.4232","url":null,"abstract":"Infecting to Melissococcus plutonius, the primary cause of the European foulbrood (EFB) disease, can be followed by infecting to the secondary bacteria, such as Enterococcus faecalis and Brevibacillus laterosporus. The aim of this research was to diagnose EFB disease by tracking the causes of the disease in apiaries all over Iran. From 260 apiaries, honey bee samples were randomly collected. After samples preparation, the genomic DNA was extracted and specific primers were selected for interested bacteria. Using the conventional polymerase chain reaction (PCR) method for E. faecalis and B. laterosporus and nested-PCR method for M. plutonius, the target fragments were amplified. Desired standard bacteria and distilled water were used as positive and negative controls, respectively. Results showed that out of 260 samples from apiaries, 74 and three samples were positive for E. faecalis and B. laterosporus bacteria, respectively. Also, the results of nested-PCR showed that 58 samples were positive, of which only 12 samples were positive in the evaluation of E. faecalis. Results demonstrated that the highest and the least levels of the infection for M. plutonius and E. faecalis were in the south and east of the country, respectively. Results indicated that sometimes due to the excessive growth of secondary bacteria, the main bacteria can be removed from the environment. Also, findings proposed that those provinces with higher number of populations, followed by higher amount of air pollution, had more infected samples than others.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog. 两种严重的情况，一种突然丧失：狗的肠系膜扭转和免疫双丝虫炎。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023716.4197

Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović

{"title":"Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog.","authors":"Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović","doi":"10.30466/vrf.2024.2023716.4197","DOIUrl":"10.30466/vrf.2024.2023716.4197","url":null,"abstract":"Sudden death has been thoroughly well defined and investigated in human medicine and its definition is not as rigorously established in veterinary medicine. In this case a 7-year-old German Shepherd was brought to the Small Animals Clinic of the Faculty of Veterinary Medicine at the University of Belgrade in very poor state. Despite treatment, the dog died 15 min after being admitted to the clinic. Since poisoning was suspected, the owners requested an autopsy. The dog was taken to the Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Belgrade for necropsy. Pathomorphological examinations revealed rupture of the mesentery with mesenteric volvulus. In addition, a large number of adult forms of Dirofilaria immitis were found in the right atrium, ventricle and in truncus pulmonalis. Further examinations of the respiratory system revealed clusters with partially degraded adult parasites in the lobes. The dog died due to mesenteric volvulus which was a rare finding, with an even rarer finding that was lung nodules with adult D. immitis parasites.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"125-128"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram? 共同补充锌、锰和铜对公羊血浆睾酮、精子质量和抗氧化酶活性有影响吗？

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2025806.4222

Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic

{"title":"Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram?","authors":"Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic","doi":"10.30466/vrf.2024.2025806.4222","DOIUrl":"10.30466/vrf.2024.2025806.4222","url":null,"abstract":"Adding trace minerals to animal diets has a positive impact on reproductive performance. This study aimed to investigate whether supplementing zinc, manganese, and copper affects the testosterone level, sperm quality, and anti-oxidant enzyme activities in rams. Ten mature Afshari rams (2.50 years old; weighing 100 ± 3.29 kg) were fed a nutritionally adequate diet for 11 weeks, half of which receiving a sulfated zinc, manganese, and copper in their daily concentrates (supplemented group), while the other half served as a control group receiving no mineral in the concentrates. Ejaculate volume, as well as sperm concentration, motility, viability, morphology, and membrane integrity were assessed. From week five onwards, all parameters were significantly higher in the supplemented group. Additionally, the percentage of abnormal sperm was lower, anti-oxidant enzymes activities in the seminal plasma were improved, and plasma testosterone concentration was higher in the supplemented group compared to the control group. However, alkaline phosphatase activity was not significantly different. Furthermore, seminal plasma concentrations of copper, zinc, and manganese at the end of the study were higher in the supplemented group compared to pre-treatment levels, while these parameters were decreased in the control group. Overall, co-supplementing copper, zinc, and manganese in rams maintained the sperm quantity and quality, as well as seminal plasma anti-oxidant capacity, emphasizing the importance of studied elements in ram reproduction.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"167-174"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep. 正常格泽勒羊脉搏波多普勒超声心动图正常值。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2033315.4333

Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh

{"title":"Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep.","authors":"Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh","doi":"10.30466/vrf.2024.2033315.4333","DOIUrl":"10.30466/vrf.2024.2033315.4333","url":null,"abstract":"Echocardiography is a noninvasive, highly sensitive method for evaluating heart disease in veterinary medicine, crucial for measuring cardiac structures and functions. Despite its importance, limited data exist on sheep, particularly the Ghezel breed, a significant breed in Iran. This study aimed to establish normal echocardiographic values for Ghezel sheep, providing reference data for both clinical and research applications. We evaluated 15 healthy Ghezel sheep with a mean age of 16.50 ± 1.80 months and a mean weight of 42.69 ± 4.73 kg. Comprehensive clinical exams, biochemical and hematological tests and color Doppler echocardiography were performed. Descriptive results from standard right and left parasternal views in both longitudinal and transverse planes were obtained and compared to existing studies. The study successfully established pulse wave Doppler reference values for the Ghezel breed. These findings could be used in diagnosing heart disease in Ghezel sheep and would be beneficial in future research particularly in sheep used as an animal model for translation in human cardiac studies.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"229-234"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric, histometric and elemental profile of the metacarpal and metatarsal bones in adult Sanjabi sheep. 成年桑贾比羊掌骨和跖骨的形态计量学、组织计量学和元素特征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-05 DOI: 10.30466/vrf.2024.2034586.4349

Sajedeh Azizi, Nader Goodarzi, Saeed Ghaderi

{"title":"Morphometric, histometric and elemental profile of the metacarpal and metatarsal bones in adult Sanjabi sheep.","authors":"Sajedeh Azizi, Nader Goodarzi, Saeed Ghaderi","doi":"10.30466/vrf.2024.2034586.4349","DOIUrl":"10.30466/vrf.2024.2034586.4349","url":null,"abstract":"Bone structure has been widely studied in mammals, however, osteon structure in sheep has received relatively little attention, especially in terms of its location on the forelimbs and hindlimbs. The aim of this study was to investigate the histometric characteristics and mineral composition of the metacarpus and metatarsus of adult Sanjabi sheep. Metacarpal and metatarsal bones were collected from five adult Sanjabi sheep (n = 10). Morphometric measurements were performed on computed tomographic scan images. Histometric parameters were measured on histological sections. The mineral composition of the bone samples was detected using the X-ray fluorescence method. The diameter of the Haversian canal in the right metatarsus was significantly greater than that in the other bones. The smallest diameter of the Haversian canal was observed for the right metacarpus. The diameter and area of the osteons in the right metacarpal were significantly greater than those in the other bones. The amount of essential mineral elements was not significantly different among bones. Aluminum and lead were significantly greater in the left metatarsus. The highest amount of copper was observed in the left metacarpus. These results indicated that there was a greater load on the right limb. This compensatory mechanism might be used to put more weight on the right forelimb and reduce the pressure caused by the weight of the rumen on the left forelimb. However, to prove this hypothesis, more detailed and extensive studies are needed in the future.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"293-300"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An outbreak of Fusobacterium necrophorum associated foot rot in a herd of four-horned antelope (Tetracerus quadricornis) in India. 印度一群四角羚羊（四角羚羊）爆发与坏死性梭杆菌相关的足腐病。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2028598.4269

Susitha Rajkumar, Loveleen Maria Vaz, Mathavan Anandhi, Karikalan Mathesh, Shivasharanappa Nayakvadi, Solomon Rajkumar, Shirish Dadarao Narnaware

{"title":"An outbreak of Fusobacterium necrophorum associated foot rot in a herd of four-horned antelope (Tetracerus quadricornis) in India.","authors":"Susitha Rajkumar, Loveleen Maria Vaz, Mathavan Anandhi, Karikalan Mathesh, Shivasharanappa Nayakvadi, Solomon Rajkumar, Shirish Dadarao Narnaware","doi":"10.30466/vrf.2024.2028598.4269","DOIUrl":"https://doi.org/10.30466/vrf.2024.2028598.4269","url":null,"abstract":"An outbreak of contagious foot rot due to Fusobacterium necrophorum infection was reported in a herd of four-horned antelopes (Tetracerus quadricornis) in a wildlife sanctuary at Goa, India. The infected animals showed signs of anorexia, limping and foot lesions, like erosions and sloughing of hooves. Mortality was recorded in two antelopes in which gross lesions, like generalized congestion of skeletal muscles and internal organs, and multi-focal to coalescing abscesses in lung, pleura, and diaphragm were observed. Histopathological evaluation of interdigital skin showed zones of coagulative necrosis, infiltration of mononuclear inflammatory cells, congestion, hemorrhage, and thrombosis in the dermis. Lung showed extensive areas of consolidation with alveolar edema, hyperemic alveolar capillaries, hemorrhage, and infiltration of inflammatory cells. The bacterial culture from heart blood, lung, liver, and kidney tissues revealed isolation of F. necrophorum being also confirmed by polymerase chain reaction (PCR) using amplification of lktA gene of F. necrophorum. In addition, the PCR also confirmed the presence of F. necrophorum DNA in the interdigital skin, lung, and liver tissues. Phylogenetic analysis showed that the F. necrophorum isolate from the present study grouped closely with isolates from Australia, China, Pakistan, and Iraq, as well as an Indian isolate from goat. The affected live animals were treated with oral antibiotics, anti-inflammatory medicines, and vitamin supplements for seven days, and recovered. This study confirmed the first outbreak of contagious foot rot and systemic infection with F. necrophorum in a herd of four-horned antelopes in India.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"51-56"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran. 伊朗西阿塞拜疆牛和水牛溶血性曼海姆病分离株的基因分型和系统发育分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2027790.4254

Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh

{"title":"Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran.","authors":"Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh","doi":"10.30466/vrf.2024.2027790.4254","DOIUrl":"https://doi.org/10.30466/vrf.2024.2027790.4254","url":null,"abstract":"This study was conducted in West Azerbaijan province, Iran (37°27'18.022\" N, 45°0'0\" E) to investigate the genotyping and phylogenetic characterization of Mannheimia haemolytica in cattle and buffaloes from November 2022 to January 2024. Mannheimia haemolytica is a bacterium known to cause pasteurellosis pneumonia, a respiratory disease in ruminants, such as cattle and sheep. This is one of the main causes of economic losses in the feedlot industry. In addition to the deaths, treatment costs are also significant. The lung and nasal swab samples were collected from 378 cattle and buffaloes. The M. haemolytica was detected in 32 (8.46%) of the samples, with a notably higher isolation rate from lung tissue (56.25%; n = 18) compared to the nasal swabs (43.75%; n = 14). Interestingly, the study also revealed a seasonal pattern, with the highest isolation rates observed during January, February, and March. Multi-locus sequence typing demonstrated that all isolates belonged to sequence type 1 (ST1) within clonal complex 28. This finding is consistent with the global prevalence of ST1 in bovine isolates, indicating widespread distribution. Phylogenetic analysis revealed a strong correlation between ST1 and STs 30 and 54, highlighting the prevalence of ST1 in M. haemolytica among ruminants in West Azerbaijan, Iran. Further research is needed to investigate its potential for causing disease and its transmission pattern.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"11-18"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on syndemic infection of porcine reproductive and respiratory syndrome virus with porcine circovirus 2 in backyard pigs of Mizoram, India. 印度米佐拉姆省散养猪群猪繁殖与呼吸综合征病毒与猪圆环病毒 2 综合感染研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023688.4187

Amitava Paul, Tridib Kumar Rajkhowa, Kiran Jayappa

{"title":"Studies on syndemic infection of porcine reproductive and respiratory syndrome virus with porcine circovirus 2 in backyard pigs of Mizoram, India.","authors":"Amitava Paul, Tridib Kumar Rajkhowa, Kiran Jayappa","doi":"10.30466/vrf.2024.2023688.4187","DOIUrl":"10.30466/vrf.2024.2023688.4187","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus 2 (PCV2) are among one of the most prevalent and important infectious agents, which cause a severe economic impact on pig farming worldwide. Circulation of these viruses together in the same pig population may lead to the syndemic infection with altered pathogenicity and thereby pathology and clinical manifestation of the diseases. The present study evaluated syndemic infection between PRRSV and PCV2 in the pig population reared in the backyard farms in Mizoram state of India. The syndemicity between PRRSV and PCV2 was confirmed by clinico-pathological studies followed by simultaneous detection of both the viruses in tissue samples by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR), immunohisto-chemistry and SYBR green-based real-time PCR / RT- PCR. The syndemicity resulted to a more severe respiratory disease in affected pigs. Pathological studies on affected pigs revealed a combined picture of hemorrhagic lesions with lymphadenopathy. The SYBR green-based absolute quantification assay estimated the PCV2 load in more quantities than that of PRRSV in all the tissues. The highest PRRSV load was detected in the lungs, while the highest PCV2 load was detected in mesenteric and inguinal lymph nodes. The co-circulation of different pathogenic viruses and their persistent infections in a population is always a matter of great concern to pig producers across the world. The present findings highlighted the similar situation with syndemic infection of PRRSV with PCV2 for the first time in Mizoram, India, that caused severe respiratory disease in affected pigs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"81-88"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic profiling of pseudorabies virus-infected PK-15 cells based on 4D label free analysis. 伪狂犬病毒感染PK-15细胞的蛋白质组学分析

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2026963.4239

Shuaiwei Wang, Xiaoyong Chen, Houqiang Luo, Meng Wang, Junyu Liu, Qingsong Han

{"title":"Proteomic profiling of pseudorabies virus-infected PK-15 cells based on 4D label free analysis.","authors":"Shuaiwei Wang, Xiaoyong Chen, Houqiang Luo, Meng Wang, Junyu Liu, Qingsong Han","doi":"10.30466/vrf.2024.2026963.4239","DOIUrl":"10.30466/vrf.2024.2026963.4239","url":null,"abstract":"Pseudorabies virus (PRV) heavily depends on host machinery to support its life cycle. Investigating the interaction between PRV and host could aid in the understandings of viral pathogenesis. In this study, we performed a 4D label free proteomic method to examine the differentially expressed proteins in porcine kidney PK-15 cells with PRV infection. The results showed that the levels of 661 proteins were significantly elevated and 693 proteins were markedly reduced. Furthermore, these altered proteins were primarily enriched in spliceosome, protein processing in endoplasmic reticulum (ER), RNA transport, and protein export. To ensure the reliability of the proteomic results, the protein levels of formin binding protein 11 and wolfram syndrome 1as components of spliceosome and ER were verified via western blotting and the results were consistent. Together, our data shed light on a new protein profiling induced by PRV infection and highlighted the importance of spliceosome and ER in PRV replication which could promote understandings of host-PRV interplay.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"141-147"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of using a Rovabio^® Plus multi-enzyme on production and incubation performance, blood parameters and duodenum morphology of broiler breeders at age of 45 - 60 weeks. Rovabio®Plus复合酶对45 ~ 60周龄肉种鸡产量、孵化性能、血液参数和十二指肠形态的影响

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2033379.433

Payam Panahi Moghaddam, Yahya Ebrahimnezhad, Naser Maheri-Sis, Habib Aghdam Shahryar, Abolfazl Aghajanzadeh-Golshani, Abolfazl Gorbani

{"title":"The effects of using a Rovabio® Plus multi-enzyme on production and incubation performance, blood parameters and duodenum morphology of broiler breeders at age of 45 - 60 weeks.","authors":"Payam Panahi Moghaddam, Yahya Ebrahimnezhad, Naser Maheri-Sis, Habib Aghdam Shahryar, Abolfazl Aghajanzadeh-Golshani, Abolfazl Gorbani","doi":"10.30466/vrf.2024.2033379.433","DOIUrl":"10.30466/vrf.2024.2033379.433","url":null,"abstract":"The aim of this study was to investigate the effects of using a Rovabio® Plus multi-enzyme on production and reproductive performance, duodenum morphology and biochemical parameters in 45 - 60 weeks old broiler breeders. For this purpose, 260 broiler breeders of Ross 308 strain were used in a completely randomized design with four treatments and five replications (13 hens and one rooster in each replication). Experimental treatments included: 1) Positive control diet (diet with standard energy and phosphorus and without multi-enzyme), 2) Negative control diet (diet in terms of energy 5.00% and phosphorus 50.00% less than the positive control diet, 3) Negative control diet with 1.00 g kg-1 Rovabio® Plus multi-enzyme and 4) Positive control diet with 1.00 g kg-1 Rovabio® Plus multi-enzyme. The results showed that negative control diet caused a significant decrease in the egg production percentage, egg weight, egg mass, settable eggs, fertile eggs, hatchability and one grade chickens, increased the feed conversion ratio and embryonic losses. Addition of 1.00 g kg-1 of multi-enzyme to negative control diet made the above parameters similar to the positive control treatment, however, the positive control treatment supplemented with multi-enzyme had a better performance compared to other treatments. This research showed that dietary supplementation of Rovabio® Plus multi-enzyme in broiler breeders at the age of 45 - 60 weeks led to the improvement of productive performance and incubation in negative control diet.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"245-252"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0