Veterinary Research Forum最新文献_第5页

Crimean-Congo hemorrhagic fever, transfer patterns, vectors and history in Iran and neighboring countries. 克里米亚-刚果出血热、传播模式、病媒以及在伊朗及其邻国的历史。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2022437.4162

Mousa Tavassoli, Salar Zarrabi Ahrabi, Gurkan Akyildiz, Aysen Gargili Keles

{"title":"Crimean-Congo hemorrhagic fever, transfer patterns, vectors and history in Iran and neighboring countries.","authors":"Mousa Tavassoli, Salar Zarrabi Ahrabi, Gurkan Akyildiz, Aysen Gargili Keles","doi":"10.30466/vrf.2024.2022437.4162","DOIUrl":"10.30466/vrf.2024.2022437.4162","url":null,"abstract":"Crimean-Congo hemorrhagic fever (CCHF) is indeed to be considered as one of the most significant vector-borne diseases globally. The virus responsible for CCHF can persist in various animals and lead to severe infections in humans. Ticks of the Ixodidae family are the acknowledged vectors of CCHF virus (CCHFV) transmission to humans. In this review, different tick species from Iran and its neighbors and their roles in the transmission of CCHFV were evaluated. However, the transmission patterns of CCHF to humans primarily involve tick bites, direct contact with the blood, organs, or tissues of contaminated animals, and exposure to CCHF patients are important in virus transmission. The results of this review confirm that the tick vectors in Iran and its neighbors are very common. Even though tick bite is one of the most important risk factors for CCHF, it cannot explain all cases, and there are other important risk factors, such as high-risk occupations and having contact with livestock. Ticks that have been proven to transmit the disease, especially Hyalomma marginatum, are scattered in Iran and neighboring countries. Transporting passengers and livestock and sometimes smuggling livestock are the means of transmitting the virus between neighboring countries. Therefore, there is a need for joint preparedness and response programs to prevent and manage CCHF between Iran and its neighbors.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"575-582"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of multi-enzymes supplementation to wheat and soybean meal-based feeds on growth performance, digestibility and carcass characteristics of quails. 在以小麦和豆粕为基础的饲料中添加多种酶对鹌鹑生长性能、消化率和胴体特征的影响

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2008986.3971

Mehmet Irmak, Muzaffer Denli, Veysi Kayri, İsa Coşkun

{"title":"Effects of multi-enzymes supplementation to wheat and soybean meal-based feeds on growth performance, digestibility and carcass characteristics of quails.","authors":"Mehmet Irmak, Muzaffer Denli, Veysi Kayri, İsa Coşkun","doi":"10.30466/vrf.2024.2008986.3971","DOIUrl":"10.30466/vrf.2024.2008986.3971","url":null,"abstract":"Japanese quail (Coturnix coturnix japonica) is a popular experimental animal model in scientific research. The present study investigated the effects of dietary multiple enzyme supplementation on growth performance, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails fed diets based on wheat and soya bean meal. A total number of 192 1-day-old quails were assigned to three treatments with 16 replicates in each and four quails per replicate for 38 days. The control group received a basal diet, and the treatment groups received a basal diet with 0.10 or 0.20% multi-enzyme, respectively. Growth performance parameters, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails were evaluated. Dietary supplementation of multi-enzymes to diet significantly increased body weight gain and improved the feed conversion rate. Moreover, quails fed with 0.10 or 0.20% multi-enzymes showed better ash digestibility coefficients and apparent metabolizable energy coefficients than the control quails. Furthermore, quails fed on a diet containing 0.20% multi-enzyme had the highest crude fiber digestibility. The villi length and the villi length/crypt depth ratio of the duodenum were significantly increased and the crypt depth was decreased in quails-fed diets supplemented with both multi-enzyme levels. However, feed consumption, carcass yield, carcass weight, heart weight, gizzard weight, liver weight and total intestine weights were not affected by treatments. In conclusion, our results showed that dietary supplementation of multi-enzymes to a wheat and soybean meal-based diet enhanced the growth performance and nutrient digestibility of quails.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"583-590"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and implementation of a TaqMan^® real-time PCR method for detection and quantification of bovine leukemia virus. 设计并实施用于检测和定量牛白血病病毒的 TaqMan® 实时 PCR 方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2016741.4084

Hassan Vahidi Emami, Arash Ghalyanchi Langeroudi, Seyed Masoud Hosseini, Hamideh Najafi

{"title":"Design and implementation of a TaqMan® real-time PCR method for detection and quantification of bovine leukemia virus.","authors":"Hassan Vahidi Emami, Arash Ghalyanchi Langeroudi, Seyed Masoud Hosseini, Hamideh Najafi","doi":"10.30466/vrf.2024.2016741.4084","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016741.4084","url":null,"abstract":"The bovine leukemia virus (BLV) is an important infectious agent transmitted from cattle to humans. It is considered one of the oncogenic viruses in breast cancer, so an accurate detection of this virus is important. The study aimed to design a specific and sensitive method based on TaqMan® real-time polymerase chain reaction (RT-PCR) for BLV detection. Probes and primers were designed using bioinformatics software for a 108 pairs region of the BLV tax gene. Criteria employed for determining analytical sensitivity were prepared using in-vitro RNA transcriptions. The National Center for Biotechnology Information (NCBI), basic local alignment search tool (BLAST) databases various viral panels and genomic samples from healthy individuals (Qom Province, Iran in 2023) were used to verify analytical specificity and clinical specificity, respectively. This method can measure a minimum of 10 copies of DNA and RNA mL-1. Moreover, the assay is linear in the range of 100 - 109 copies mL-1. By testing negative specimens, the method specificity was 100%. The reproducibility results of the reaction were examined at the intra- and inter-assay comparison. In fact, 10 technical replicates of each concentration of the control sample were analyzed in each working reaction. Due to the locally made kit, exact sensitivity and specificity, rapid analysis, and relatively low cost, as compared to commercial kits of other countries, the method introduced in the present study could be suitable for accurate detection of the BLV. Also, the TaqMan® real-time PCR method could be detected in cattle and human and before malignant changes of breast cancer which could reduce infection and breast cancer.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"417-423"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal protection by ellagic acid in a rat model of glycerol-induced acute kidney injury. 鞣花酸在甘油诱发急性肾损伤大鼠模型中对肾脏的保护作用

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2000658.3859

Maryam Khombi Shooshtari, Alireza Sarkaki, Mohammad Rashno, Khojasteh Hoseinynejad

{"title":"Renal protection by ellagic acid in a rat model of glycerol-induced acute kidney injury.","authors":"Maryam Khombi Shooshtari, Alireza Sarkaki, Mohammad Rashno, Khojasteh Hoseinynejad","doi":"10.30466/vrf.2023.2000658.3859","DOIUrl":"10.30466/vrf.2023.2000658.3859","url":null,"abstract":"Studies conducted on animal models have shown that the administration of glycerol can lead to kidney tissue damage and impaired renal function. This is believed to be caused by oxidative stress and inflammation, which in turn can result in elevated levels of blood urea nitrogen (BUN) and creatinine. These metabolites are commonly used as indicators of renal function. The aim of the current experimental research was to investigate the protective efficacy of ellagic acid in a rat model of rhabdomyolysis induced by glycerol. Sixty healthy adult male Wistar rats weighing between 250 - 300 g were divided into five equal groups including control, rhabdomyolysis (administered 8.00 mL kg-1 of glycerol), and three rhabdomyolysis plus various doses of ellagic acid (25.00, 50.00 and 100 mg kg-1 per day; 72 hr after receiving glycerol for 14 days successively) groups. Serum levels of BUN, creatinine, lactate dehydrogenase, alkaline phosphatase, electrolytes and inflammatory cytokines were evaluated in all rats. Histopathological studies were also performed on kidney tissues from all groups. The administration of ellagic acid resulted in a significant increase in renal function biomarkers compared to the rats with acute kidney injury. This increase was consistent with notable reductions in tumor necrosis factor-α levels and increases in interleukin-10 levels observed in blood samples. Furthermore, the improvement in histopathological indices observed in rats received ellagic acid confirmed its nephroprotective role. The results of the current experimental study suggest that ellagic acid can improve kidney damage following glycerol injection, potentially by modulating the inflammatory process.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"105-111"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence spectroscopy and molecular modeling studies on the interaction of aflatoxin B1 and G1 with bovine α-lactalbumin. 黄曲霉毒素 B1 和 G1 与牛α-乳白蛋白相互作用的荧光光谱学和分子模型研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2006492.3959

Omid Soltanabadi, Maliheh Sadat Atri, Mohammad Bagheri

{"title":"Fluorescence spectroscopy and molecular modeling studies on the interaction of aflatoxin B1 and G1 with bovine α-lactalbumin.","authors":"Omid Soltanabadi, Maliheh Sadat Atri, Mohammad Bagheri","doi":"10.30466/vrf.2024.2006492.3959","DOIUrl":"10.30466/vrf.2024.2006492.3959","url":null,"abstract":"Aflatoxins are toxic chemicals produced by Aspergillus fungi. Reports exist on the relationship of aflatoxin exposure via contaminated food and feed to hepatotoxicity and liver cancer. Aflatoxin B1 (AFB1) and Aflatoxin G1 (AFG1) are two dangerous types of aflatoxins for human health. Bovine α-lactalbumin (ALA) is the second major whey protein in milk which bear diverse biological functions. In this study, the interaction of AFB1 and AFG1 with the ALA protein was studied using fluorescence spectroscopy, molecular docking and molecular dynamic (MD) simulation. The spectroscopy experiments showed that the interaction with AFB1 and AFG1significantly quenched the intrinsic fluorescence emission of ALA via a static quenching mechanism. The free energy of binding and binding constant (Ka) obtained from the intrinsic fluorescence results were -5.32 kcal per mol and 0.80 × 104 L mol-1 for AFB1 and -5.64 kcal per mol and 1.35 × 104 l mol-1 for AFG1, respectively. Molecular docking studies were conducted before and after the MD simulation to estimate the binding sites, Ka s and binding mode. Results from the molecular docking showed that AFB1 and AFG1 bound to ALA via hydrophobic interaction and hydrogen bond. After MD simulation, the precision of the Ka obtained from the docking results was improved and it was more similar to the experimental results of fluorescence spectroscopy. Other simulation results were aligned well with the molecular docking and fluorescence spectroscopy results. Accordingly, AFB1and AFG1 could form complex with ALA, however, AFG1 showed higher affinity for binding to ALA and more compact complex structure.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"551-558"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Susceptibility of Pasteurella multocida isolated from cattle in Egypt to antibiotics, silver, chitosan and curcumin nanoparticles. 埃及牛群中分离出的多杀性巴氏杆菌对抗生素、银、壳聚糖和姜黄素纳米颗粒的敏感性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2017140.4090

Amany Dieb Bahr, Emil Saad Abdel Massieh

{"title":"Susceptibility of Pasteurella multocida isolated from cattle in Egypt to antibiotics, silver, chitosan and curcumin nanoparticles.","authors":"Amany Dieb Bahr, Emil Saad Abdel Massieh","doi":"10.30466/vrf.2024.2017140.4090","DOIUrl":"10.30466/vrf.2024.2017140.4090","url":null,"abstract":"Pasteurella multocida is a Gram-negative bacterium causing economically significant diseases in cattle. This study aimed to determine P. multocida susceptibility to different antibiotics and antibiotic alternatives. In this study, 246 samples (180 nasal swabs and 66 lung tissue specimens) were collected from cattle showing respiratory manifestations in Egypt. Suspected P. multocida colonies following culture were subjected to polymerase chain reaction (PCR) for molecular confirmation of the isolates. A multiplex PCR was employed to identify P. multocida capsular groups. Susceptibility of the isolated P. multocida to different antibiotics and nanoparticles as antibiotic alternatives including silver (AgNPs), chitosan (CNPs) and curcumin (CurNPs) were tested using broth microdilution method. Thirty-two P. multocida isolates were obtained, kmt1 gene was detected in these isolates, and molecular capsular types classification revealed that all isolates were belonged to the capsular type A. Based on broth microdilution method findings, 20 (62.50%) isolates were considered as multi-drug resistant (MDR); the isolates were most sensitive to danofloxacin and kanamycin, whereas they were most resistant to doxycycline and tilmicosin. Antibiotic alternatives showed high anti-microbial activity against tested isolates with minimum inhibitory concentrations ranging from 1.56 - 6.25 μg mL-1, 156 - 625 μg mL-1, and 128 - 512 μg mL-1 for AgNPs, CNPs and CurNPs, respectively. Our finding demonstrated that MDR P. multocida was evident in cattle in Egypt. Although antibiotic alternatives showed promising in vitro anti-microbial effects against MDR isolates, additional studies are required to be actually applicable in veterinary practices.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"455-462"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran. 筛查伊朗西北部牛、绵羊和山羊中分离出的耐甲氧西林金黄色葡萄球菌的万古霉素抗性相关基因。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2003964.3902

Leila Maleki, Amir Tukmechi

{"title":"Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran.","authors":"Leila Maleki, Amir Tukmechi","doi":"10.30466/vrf.2023.2003964.3902","DOIUrl":"10.30466/vrf.2023.2003964.3902","url":null,"abstract":"Staphylococcus aureus is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of S. aureus. The MecA gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely vanA and vanB, were searched in the isolates. Out of 400 nasal swabs, 69 samples had S. aureus; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"159-164"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages. 不同胚胎和胎儿阶段鸵鸟（Struthio camelus）气管和肺的形态发生。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2023-06-15 DOI: 10.30466/vrf.2023.2009800.3984

Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar

{"title":"Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages.","authors":"Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar","doi":"10.30466/vrf.2023.2009800.3984","DOIUrl":"10.30466/vrf.2023.2009800.3984","url":null,"abstract":"The ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock industry. This research was conducted to analyze the development of the ostrich respiratory system during fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms and then incubated at 36.00 - 37.00 ˚C and 25.00 ± 2.00% humidity for 40 days. Sections were taken on days 13, 22, 26, 30, 36, and 42 of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by Hematoxylin and Eosin, Alcian Blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques. It was concluded that the trachea in the 13-day-old embryo and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilage and differentiation of mesenchymal to the loose connective tissue. The bronchial stage of the lung was observed in the 22-day-old embryo, pseudoglandular stage in the 26-day-old embryo, and parabrachial and air capillary stage in the 30-day-old embryo. The information obtained from this study will be useful for diagnosing pathologies affecting this vital system and results in improving industrial breeding management.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"297-301"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after spinal cord injury. 立即注射 hTERT-间充质干细胞-IDO1-EVs可降低脊髓损伤后的低白蛋白血症。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2003942.3903

Shiva Amanollahi, Ahmad Reza Bahrami, Azadeh Haghighitalab, Hanieh Shaterzadeh Yazdi, Hossein Kazemi Mehrjerdi

{"title":"Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after spinal cord injury.","authors":"Shiva Amanollahi, Ahmad Reza Bahrami, Azadeh Haghighitalab, Hanieh Shaterzadeh Yazdi, Hossein Kazemi Mehrjerdi","doi":"10.30466/vrf.2023.2003942.3903","DOIUrl":"10.30466/vrf.2023.2003942.3903","url":null,"abstract":"Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human indoleamine 2,3-dioxygenase-1 (IDO1) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of 72-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and IDO1-EVs. The 60.00 µL of hydrogel or hydrogels containing 100 µg sEVs from GFP or IDO1-EVs were locally injected immediately after SCI (laminectomy of the T10 vertebra and clip compression). After 8 weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received IDO1-EVs (3.52 ± 0.04) was increased in comparison with the SCI group (3.00 ± 0.94). Also, these animals indicated higher glucose levels in their serum (250.17 ± 69.61) in comparison with SCI ones (214 ± 45.34). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of IDO1-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"27-34"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the effects of different DHA/EPA ratios in rainbow trout (Oncorhynchus mykiss) egg composition on foregut development of larvae. 研究虹鳟鱼（Oncorhynchus mykiss）卵成分中不同的 DHA/EPA 比例对幼鱼前肠发育的影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2013685.4035

Dana Dadras, Ahmad Imani, Naser Agh, Rasoul Shahrooz, Parisa Mohammadisefat

{"title":"Investigating the effects of different DHA/EPA ratios in rainbow trout (Oncorhynchus mykiss) egg composition on foregut development of larvae.","authors":"Dana Dadras, Ahmad Imani, Naser Agh, Rasoul Shahrooz, Parisa Mohammadisefat","doi":"10.30466/vrf.2024.2013685.4035","DOIUrl":"10.30466/vrf.2024.2013685.4035","url":null,"abstract":"Lecithotrophic larvae utilize extensive yolk reserves for early development. In this study, the effect of egg docosahexaenoic acid (DHA):eicosapentaenoic acid (EPA) ratios (i.e., 5.92, 10.08, 11.66, and 14.53) on the emerging larvae foregut development of rainbow trout was examined. Larvae samples were taken from day 22 to 36 post-fertilization. Thin whole body longitudinal sections were prepared and stained by Hematoxylin and Eosin and Alcian blue procedure. The sections were examined regarding epithelial layer thickness, intestinal fold height and mucosal layer thickness along with number of enterocytes and goblet cells. Results indicated that maximum thickness of the epithelium was observed on day 36 post fertilization in larvae hatched from eggs with DHA:EPA ratios of 14.53 and 10.08. The highest and lowest intestinal folds height were also observed in larvae hatched from eggs with DHA:EPA ratios of 10.08 and 14.53, respectively. The mucosal-submucosa layer thickness was the highest in larvae hatched from eggs with DHA:EPA ratio of 10.08. Enterocyte's count was the highest in larvae obtained from eggs with DHA:EPA ratio of 10.08 on day 36 post-fertilization. The highest and lowest number of goblet cells were enumerated in larvae obtained from eggs with DHA:EPA ratios of 5.53 and 14.53, respectively. In conclusion, our results revealed that feeding rainbow trout broodstock with diet contained highly unsaturated fatty acids (HUFA):polyunsaturated fatty acids (PUFA) ratio of 0.28 could result in the egg with DHA:EPA ratio of 10.08 which in turn yielded larvae with better foregut development parameters compared to those larvae emerged from the eggs with increased DHA :EPA ratio.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"463-471"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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