Veterinary Research Forum最新文献

{"title":"Protective effect of nanocurcumin on acetaminophen-induced hepatic and renal toxicities in pigeons.","authors":"Saeed Seifi, Nasser Vajdi, Shohreh Alian Samakkhah, Navideh Mirzakhani","doi":"10.30466/vrf.2024.2011585.4009","DOIUrl":"10.30466/vrf.2024.2011585.4009","url":null,"abstract":"<p><p>In this study, the effects of nanocurcumin on acetaminophen-induced acute hepatorenal toxicity in domestic pigeons (<i>Columba livia</i>) were investigated. Fifteen pigeons were randomly assigned into three groups. Group I was served as a negative control group and received tap water as a placebo. Pigeons in groups II and III were administered acetaminophen at the beginning of the experiment (hr 0). Group III was further treated with nanocurcumin, at 12 hr after acetaminophen administration, being continued every 12 hr for two days. The birds were observed for clinical signs of acute drug toxicity. Blood samples were collected from the pigeons at hr 0, 12, 24 and 48 of the experiment for biochemical analysis of the serum. The results showed that acetaminophen toxicity increased the serum levels of aspartate aminotransferase, alanine aminotransferase, urea and uric acid in the pigeons. Nanocurcumin treatment of acetaminophen intoxicated pigeons attenuated increases in biomarkers of the liver and kidney functions towards control levels. Also, the consumption of nanocurcumin minimized histopathological changes in the liver and kidney. A mortality of 60.00% was seen in the acetaminophen-induced toxicity group; while, none of the birds treated with nanocurcumin died. It can be concluded that nanocurcumin alleviates the acetaminophen-induced acute toxic liver and kidney damages, which can lead to pigeon mortality.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"145-150"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-structural organization of the gallbladder mucous membrane of Anglo-Nubian goat.","authors":"Anna Valerievna Prusakova, Nikolay Vyacheslavovich Zelenevskiy, Aleksey Viktorovich Prusakov, Anatoly Viktorovich Yashin, Vladimir Sergeevich Ponamarev","doi":"10.30466/vrf.2024.2011081.4005","DOIUrl":"10.30466/vrf.2024.2011081.4005","url":null,"abstract":"<p><p>Currently, studies devoted to establish the anatomical and histological patterns of the internal organs organization in animals depending on their species and breed, as well as conditions of detention are the most relevant. The liver morphology in representatives of the ruminant family has not been sufficiently studied. Questions regarding the micro- and ultra-structural organizations of the gallbladder wall remain open. The aim of the study was to establish the ultra-structural organization features of the gallbladder mucous membrane of an Anglo-Nubian goat. The material for the study was the gallbladder wall's fragments of an adult Anglo-Nubian goat. Further processing of the obtained samples was carried out to acquire histological preparations. Ultra-thin sections were photographed in a Jem-1011 electron microscope at magnifications of 2,500 - 3,000. It was found that the gallbladder mucous membrane of an Anglo-Nubian goat is formed by the epithelial layer and its own lamina. The epithelial layer is represented by a single-layer prismatic epithelium. The nuclei are light, with clear contours. The apical surface of epithelial cells forms microvilli and the cytoplasm of the apical pole of cells contains many electron-dense secretory granules. The lateral surfaces of the cells in their apical part are interconnected by tight contacts. The lamina propria is formed by loose connective tissue containing many blood vessels and nerve fibers. Referring to the scientific literature describing possible pathologies of the gallbladder, we can conclude that the picture presented in the results generally corresponds to the position of the gallbladder without pathologies.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"165-169"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal dispersion and pathogenic potential of multidrug-resistant <i>Aeromonas</i> spp. isolated from <i>Oncorhynchus mykiss</i> with hemorrhagic septicemia.","authors":"Shaaban Tayar Sadiq, Alaa Hussain Ali Al-Hamdani, Zanan Mohamed-Ameen Taha","doi":"10.30466/vrf.2024.2010315.3998","DOIUrl":"10.30466/vrf.2024.2010315.3998","url":null,"abstract":"<p><p>This study was important to improve proper biosecurity measures and controlling the spread of <i>Aeromonas</i> to prevent future outbreaks. This research sought to determine whether virulent <i>Aeromans</i> species were present in morbid rainbow trout, their resistance and their genetic relatedness. A total number of 542 tissue lesion specimens were collected from gill, liver, heart and kidneys in morbid domesticated fish in Duhok province, Iraq. The <i>gyrB</i> DNA sequence analysis was used to determine the species classification. Drug susceptibility testing was conducted for all isolated strains using disc diffusion technique. The genotyping analysis was carried out using enterobacterial repetitive intergenic consensus-polymerase chain reaction. Thirty-four isolates were found and they were classified into three species (<i>Aeromonas veronii, Aeromonas sorbia,</i> and <i>Aeromonas allosaccharophila)</i>, where <i>A. veronii</i> stand as one of the most prevalent species. The most frequently affected organ by <i>Aeromonas</i> was the gills among four different organs. The detection frequencies of the virulence genes <i>aerolysin,</i> <i>outer membrane protein, glycerophospholipid-cholesterol acyltransferase, elastase, flagella,</i> <i>serine protease, cytotonic heat-</i>labile<i>,</i> and <i>hemolysin</i> were 100%, 100%, 79.41%, 64.70%, 76.47%, 67.64%, 70.58%, and 41.17, respectively. None of the strains possessed all of the virulence markers. All isolates were completely resistant to ceftazidime, amoxicillin and doxycycline. All isolates were found to be multi-drug-resistant. Regardless of the nearest geographic source area of samples and the same <i>Aeromonas</i> species, there was a high genetic diversity. The results of this study could help farmers and researchers make informed decisions about measures of biosecurity and proper therapeutic drugs to apply to prevent current outbreaks and prevent them from recurring again.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"529-536"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.","authors":"Zohreh Mostahsan, Saeed Azizi, Ali Soleimanzadeh, Ali Shalizar-Jalali","doi":"10.30466/vrf.2024.2024815.4202","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024815.4202","url":null,"abstract":"<p><p>Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of unscavenged reactive oxygen species. This study aimed to investigate the effects of intra-peritoneal administration of Mito-TEMPO (MT) on I/R injury in testicular torsion/detorsion (T/D) in mice. Forty-two male mice were divided into seven groups including 1 control and 6 treatment groups (360° T/D, 720° T/D, 360° T/D + 0.70 mg kg^-1 MT, 360° T/D + 1.00 mg kg^-1 MT, 720° T/D + 0.70 mg kg^-1 MT, and 720° T/D + 1.00 mg kg^-1 MT). After inducing 360° and 720° clockwise testicular torsions for 2 hr, sperm parameters, apoptosis-related genes expression, and <i>in vivo</i> fertility index were evaluated. The results showed that 720° T/D can lead to increased abnormal sperm morphology, sperm DNA damage, and <i>Bax</i> expression, while the <i>Bcl-2</i> expression was reduced compared to the other groups. In addition, it also had negative effects on sperm total and progressive motilities as well as viability and plasma membrane functionality (PMF). The results also showed that administration of MT to T/D mice can result in a reduction in abnormal sperm morphology, DNA damage, and <i>Bax</i> expression. It could also increase sperm total and progressive motilities, viability and PMF, <i>Bcl-2</i> expression, and <i>in vivo</i> fertility index. Based on our results, it is concluded that MT, when administered after spermatic cord torsion in mice, provides significant protection against acute testicular T/D injury.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"665-672"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability of guinea pig (<i>Cavia porcellus</i>) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C.","authors":"Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad","doi":"10.30466/vrf.2024.2023686.4186","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023686.4186","url":null,"abstract":"<p><p>The cooling procedure markedly diminishes the quality of guinea pig (<i>Cavia porcellus</i>) sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions. This study aimed to assess the viability of guinea pig spermatozoa diluted in Tris buffer-based extenders during storage at 5.00 ˚C. Sperm collection was carried out through castration of the animals. For this study, 10 adult male guinea pigs were utilized, being divided into four groups including phosphate-buffered saline (PBS), human tubal fluid (HTF), Tris-citric-glucose (TCG), and Tris-fructose-yolk (TFY) cultures. Evaluations including sperm motility, morphology, plasma membrane integrity, viability, and total count were conducted at 0, 24, and 48 hr after sampling. The results obtained indicated that at the 24-hr and 48-hr marks of the experiment, both overall and progressive motility percentages, viability, plasma membrane integrity, and morphology of sperms in the PBS and HTF cultures exhibited a significant increase in comparison with the TCG and TFY cultures. Consequently, it can be inferred that PBS and HTF cultures are highly effective in preserving the quality of guinea pig spermatozoa.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"691-696"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses.","authors":"Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2023.2004451.3910","DOIUrl":"10.30466/vrf.2023.2004451.3910","url":null,"abstract":"<p><p>The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg^-1) in combination with acepromazine maleate (0.05 mg kg^-1) intravenously (IV). In treatment II (T2), the animals received intra-gastric administration of clonidine (0.002 mg kg^-1) followed by acepromazine (0.05 mg kg^-1; IV) after 60 min. Head height above the ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 min before and 5, 15, 30, 60, and 90 min after drug administration. In T2, recordings were made 5 min before clonidine, 55 min after clonidine administration, and then 5, 15, 30, 60, and 90 min after acepromazine injection. Analyses of the data showed there were not significant differences regarding HHAG and echocardiographic indices between two treatments. For sedation of healthy horses, it was concluded that intra-gastric administration of clonidine and IV administration of acepromazine showed similar sedative and cardiovascular effects compared to IV acepromazine-xylazine administration.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"21-26"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system.","authors":"Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2024.2015987.4069","DOIUrl":"https://doi.org/10.30466/vrf.2024.2015987.4069","url":null,"abstract":"<p><p>Investigating the mechanisms responsible for pain processing of natural and synthetic chemical compounds is necessary to optimize pain management. Curcumin (Cur), the active ingredient of turmeric, exhibits potent analgesic and anti-inflammatory properties by employing multiple mechanisms at the local peripheral, spinal and supra-spinal levels. This study was aimed to investigate the effect of oral administration of Cur on muscle pain induced by intramuscular (IM) injection of formalin. To explore the possible local mechanisms, a cyclooxygenase (COX) inhibitor, diclofenac (Dic) and a COX product, prostaglandin E₂ (PGE₂), were applied. The IM injection of formalin (25.00 µL, 2.50%) into the gastrocnemius muscle induced two distinct phases of hind leg flinching. A short-lasting (10 min) hind leg lifting was observed following IM injection of PGE₂ (2 µg kg^-1, 25.00 µL). Oral administration of Cur (25.00 and 100 mg kg^-1) and IM injection of 40.00 µg kg^-1 Dic attenuated formalin and PGE2 induced nociceptive behaviors. Contra-lateral IM injection of Dic did not change muscle pain induced by ipsilateral IM injection of formalin and PGE₂. The second phase of formalin induced flinching as well as PGE₂ evoked lifting were more suppressed when 40.00 µg kg^-1 Dic and 100 mg kg^-1 Cur were used together. Locomotor activity was not changed by the above-mentioned treatments. It was concluded that the reducing effect of muscle pain of Cur might be related to the local inhibition of COX.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"411-416"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of the <i>beta-lactamase Cefotaxime</i> gene in <i>Escherichia coli</i> from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island.","authors":"Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati","doi":"10.30466/vrf.2024.2014818.4056","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014818.4056","url":null,"abstract":"<p><p>This study aims to detect <i>Escherichia coli</i> which encodes <i>beta-lactamase Cefotaxime</i> <i>(blaCTX-M)</i> gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of <i>E. coli</i> was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of <i>E. coli</i> which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the <i>blaCTX-M</i> gene of <i>E. coli</i> and sequences of the <i>blaCTX-M</i> gene were phylogenetically analyzed. The research results obtained three <i>E. coli</i> isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of <i>E. coli</i> was resistant to penicillin G and oxytetracycline. 66.66% of <i>E. coli</i> was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of <i>E. coli</i> was resistant to tetracycline. <i>Escherichia coli</i> isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of <i>E. coli</i> detection using PCR showed that three <i>E. coli</i> isolates encoded the <i>blaCTX-M</i> gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that <i>E. coli</i> from the reproductive tract of Bali cattle was related to <i>E. coli</i> that encoded <i>blaCTX-M-</i>14 isolated from humans.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"397-402"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of <i>Nigella sativa</i> oil, thymoquinone and dexamethasone on bleomycin-induced lung fibrosis in rats.","authors":"Farid Saghghaei, Ali Rassouli, Goudarz Sadeghi-Hashjin, Farhang Sasani, Mohammad Kazem Koohi","doi":"10.30466/vrf.2024.2024154.4196","DOIUrl":"10.30466/vrf.2024.2024154.4196","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a chronic interstitial lung disease with a progressive damage to the air sacs and deposition of collagen fibers in the lung tissue. The study aimed to explore the effects of <i>Nigella sativa</i> oil (NSO) or thymoquinone (TQ), alone or in combination with dexamethasone (DEX), on the development of bleomycin (BLM)-induced PF. Forty-two male rats were divided into seven groups: Control (CTRL); BLM, received a single dose of BLM on day 0, intratracheally; all remaining groups received BLM, as well. DEX, received DEX daily, intraperitoneally, 1 day before BLM and continued for 14 days; NSO and TQ groups, received daily NSO and TQ, respectively, 7 days before BLM and continued for 35 days; DEX + TQ, received both DEX and TQ; DEX + NSO, received both DEX and NSO. At the end, lung tissues were used for histopathological and biochemical analyses. BLM significantly increased the severity of fibrosis and inflammation compared to the CTRL. Bleomycin also significantly increased the amount of hydroxyproline, however, decreased most antioxidant enzymes in the lung tissue compared to the other groups. Group TQ + DEX significantly reduced the severity of BLM-induced PF as well as alterations in biochemical parameters, lung weight and O2 saturation. <i>Nigella sativa</i> oil slightly reduced BLM-induced PF, however, it caused non-significant hyperemia in lung tissue. Thymoquinone potentiated the effects of DEX on most biochemical and pathological alterations of BLM-induced lung injury much better than NSO. More studies are needed to support the use of NSO and TQ as potential protective agents against PF.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"613-620"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression.","authors":"Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran","doi":"10.30466/vrf.2024.2022533.4165","DOIUrl":"10.30466/vrf.2024.2022533.4165","url":null,"abstract":"<p><p>Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-142-3p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-142-3p was initially assessed in MDA-MB-468 cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-142-3p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-142-3p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX2, Octamer 4, HMGA2, Kruppel-like factor 4, and Bach-1. Additionally, the combination of miRNA-142-3p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G¹ phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-142-3p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-142-3p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"629-643"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}