Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2023.2000166.3851
Sina Tamaddonfard, Amir Erfanparast, Esmaeal Tamaddonfard, Farhad Soltanalinejad
{"title":"The CB1 cannabinoid receptors involvement in anti-epileptic effect of safranal on penicillin-induced epileptiform activity in rats.","authors":"Sina Tamaddonfard, Amir Erfanparast, Esmaeal Tamaddonfard, Farhad Soltanalinejad","doi":"10.30466/vrf.2023.2000166.3851","DOIUrl":"10.30466/vrf.2023.2000166.3851","url":null,"abstract":"<p><p>Neuroprotective effects for natural products are supported by several studies. In this regard, safranal, a constitute of saffron, has the potential to exert beneficial effects in neuro-logical disorders such as Parkinson's disease, epilepsy, stroke, multiple sclerosis and Alzheimer's disease. Here, we investigated the effect of safranal on penicillin-induced epileptiform activity. Also, the effects of intracerebroventricular (ICV) microinjection of AM251 as a CB1-cannabinoid receptors antagonist to clarify the possible mechanism of safranal were evaluated. Epileptiform activity was induced by intra-cortical administration of penicillin (300 IU, 1.50 μL) in urethane-anesthetized rats. Electrocorticographic recordings were used to analyze the frequency and amplitude of spike waves. Intraperitoneal injections of safranal at doses of 1.00 and 4.00 mg kg<sup>-1</sup> significantly reduced both the number and amplitude of spike waves. The ICV microinjection of AM251 (0.50 μg 2.00 μL<sup>-1</sup>) significantly increased the frequency and amplitude of spike waves. In addition, the anti-epileptic effect induced by administration of safranal at a dose of 4.00 mg kg<sup>-1</sup> was partially prevented by ICV microinjection of 0.50 μg 2.00 μL<sup>-1</sup> of AM251. The results showed anti-epileptiform activities for safranal. Central CB1 cannabinergic receptors might be involved in the anti-epileptiform activity of safranal.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2022.554037.3474
Lucai Wang, Lijia Liu, Huanrong Zhang
{"title":"Development and validation of an insulated isothermal polymerase chain reaction assay for the rapid detection of <i>Mycoplasma synoviae</i>.","authors":"Lucai Wang, Lijia Liu, Huanrong Zhang","doi":"10.30466/vrf.2022.554037.3474","DOIUrl":"10.30466/vrf.2022.554037.3474","url":null,"abstract":"<p><p><i>Mycoplasma synoviae</i>, which causes the disease known as chicken synovitis, causes serious immunosuppression. We developed a rapid insulated isothermal polymerase chain reaction (iiPCR) assay for on-site detection of <i>M. synoviae</i> using a primer and probe set targeting the <i>variable lipoprotein and haemagglutinin</i> (<i>vlh</i><i>A</i>) gene. In addition, the specificity, sensitivity, repeatability, and clinical detection of this method were evaluated. Our iiPCR assay detected <i>M. synoviae</i> clinical isolates and samples successfully and produced negative results on <i>Mycoplasma galliscepticum</i><i>,</i> avian viral arthritis, <i>Escherichia coli</i>, <i>Salmonella</i>, <i>Staphylococcus aureus</i> and <i>Corynebacterium</i>, indicating that the PCR reactions were specific. Additionally, our iiPCR assay detected the prepared positive standard plasmid diluted 10 times (1.00 × 10<sup>-1</sup> - 1.00 × 10<sup>-10</sup>) as a template. The undiluted positive plasmid was positive and double distilled water was negative indicating that the PCR reactions were sensitive, respectively. Finally, the <i>vlhA</i> positive standard plasmid with dilution multiple of 1.00 × 10<sup>-4</sup> - 1.00 × 10<sup>-6</sup> was repeatedly detected three times to evaluate the repeatability of the iiPCR method established in this experiment showing that the iiPCR of <i>M. synoviae</i> is repeatable. The established iiPCR was also used to detect 50 chicken joint enlargement samples. The thermostatic detection PCR established in this experiment was comparable to a reference real-time PCR (qPCR).</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"7-12"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-04-15DOI: 10.30466/vrf.2024.2020553.4141
Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk
{"title":"Congenital feline tuberculosis: the first case report.","authors":"Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk","doi":"10.30466/vrf.2024.2020553.4141","DOIUrl":"10.30466/vrf.2024.2020553.4141","url":null,"abstract":"<p><p>This study presents the first report of congenital tuberculosis in an 8-month-old male British Shorthair cat. The case was examined using histopathological and immunohistochemical methods. The cat was referred to a private veterinary clinic with general respiratory system problems and subsequent deterioration, leading to death. The cat owner granted permission for the cat necropsy and pathological examinations at Department of Pathology, Faculty of Veterinary Medicine, Selçuk University, Konya, Türkiye. During systemic necropsy, white round foci with diameters ranging from 3.00 to 5.00 mm were observed in the lung and spleen. Tissue samples were collected from the lung, spleen, liver, heart, kidney, mediastinal lymph nodes and brain for histopathological examinations. The tissues were subjected to routine histological tissue processing and sections were stained with Hematoxylin and Eosin and Ziehl-Neelsen. Additionally, <i>Mycobacterium spp</i>. antibodies were used for immunohistochemical staining. Microscopic examination revealed exudative tuberculosis lesions, areas of necrosis without a fibrous capsule and karyorrhectic cells only in the lung and spleen. Acid-resistant bacteria observed by ZN staining in the lesioned areas of the lung and spleen were identified as <i>Mycobacterium spp</i>. using immunohistochemical staining. No positive staining was observed in other organs using ZN and immunohistochemical methods. As a result, congenital tuberculosis was diagnosed in a cat for the first time, especially in relation to lesions in the spleen.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"203-205"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of thymoquinone on acetic acid-induced visceral nociception in rats: role of central cannabinoid and α<sub>2</sub>-adrenergic receptors.","authors":"Somayyeh Naderi, Esmaeal Tamaddonfard, Saeid Nafisi, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2023.2005364.3922","DOIUrl":"10.30466/vrf.2023.2005364.3922","url":null,"abstract":"<p><p>Thymoquinone (TQ) is the main biologically active substance of <i>Nigella sativa</i> (black seeds). It has anti-cancer, anti-inflammatory, anti-diabetic, anti-oxidative and anti-nociceptive properties. This study was aimed to explore the effect of TQ on acetic acid-induced visceral nociception. The central mechanisms of the effect of TQ were investigated using cannabinergic (AM251) and α<sub>2</sub>-adrenergic (yohimbine [Yoh]) antagonists. The lateral ventricle of the brain was cannulated for intracerebroventricular (ICV) injections. Visceral nociception was induced by intra-peritoneal (IP) injection of acetic acid (1.00% in a volume of 1.00 mL). Measuring the latency time to the first writhing appearance and counting the number of writhing in 5-min intervals for a period of 60 min were performed. Locomotor activity was determined using an open-field test. Oral administration (PO) of 2.50 and 10.00 mg kg<sup>-1</sup> TQ increased the latency time to the first writhing appearance and decreased the number of writhing. The AM251 (5.00 µg <i>per</i> rat; ICV) and Yoh (5.00 µg <i>per</i> rat; ICV) partially prevented TQ (10.00 mg kg<sup>-1</sup>; PO)-induced anti-nociception. Locomotor activity was not altered by these treatments. The results of the present study showed that TQ had the ability to reduce visceral nociception caused by IP injection of acetic acid. The central mechanisms of this action of TQ might be partially mediated by cannabinergic and α<sub>2</sub>-adrenegic receptors.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"131-138"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-09-15DOI: 10.30466/vrf.2024.2020333.4136
Soheil Vazifehdoust, Ali Shalizar-Jalali, Mohammad Reza Nourani, Mehrdad Moosazadeh Moghaddam, Mohsen Yazdanian
{"title":"Improvement of osteogenesis and antibacterial properties of a bioactive glass/gelatin-based scaffold using zoledronic acid and CM11 peptide.","authors":"Soheil Vazifehdoust, Ali Shalizar-Jalali, Mohammad Reza Nourani, Mehrdad Moosazadeh Moghaddam, Mohsen Yazdanian","doi":"10.30466/vrf.2024.2020333.4136","DOIUrl":"10.30466/vrf.2024.2020333.4136","url":null,"abstract":"<p><p>This study aimed to investigate the effects of zoledronic acid (ZA) and antibacterial CM11 peptide on the osteoinduction and antibacterial properties of bioactive glass (BG). The bioactive glass/gelatin (BG/Gel) composite was synthesized using the sol-gel method. The 2-x minimum inhibitory concentration of the peptide and 4.00 mg mL<sup>-1</sup> of ZA were added to the BG/Gel during fabrication. The BG/Gel composite morphological and structural characteristics and anti-bacterial activities were analyzed using Fourier transform infra-red spectroscopy, scanning electron microscopy and disk diffusion test, respectively. The release of the peptide and ZA from BG/Gel was measured using ultra-violet spectroscopy. After 14 days, the effects of the peptide/ ZA-containing BG/Gel (PZ-BG/Gel) on the growth and differentiation of mesenchymal stem cells were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, calcium and alkaline phosphatase assays, immunocytochemical staining for osteocalcin (OCN) and real-time polymerase chain reaction for <i>OCN</i>, <i>type I collagen</i>, <i>bone morphogenetic protein 2</i> and <i>Runt-related transcription factor-2</i> genes. The disk diffusion test showed the anti-microbial activity of the scaffold against multi-drug-resistant isolates of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. Analyses showed a significantly higher level of stem cells differentiation into the osteo-genic cells in PZ-BG/Gel scaffold compared to BG/Gel scaffold alone. Accordingly, osteoblast markers were significantly increased in comparison with the control. In conclusion, the osteo-induction and antibacterial properties of BG-based scaffold can be improved using ZA and CM11.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"487-498"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Curcumin alleviates inflammatory effects of ketamine anesthesia in postnatal rats.","authors":"Soroush Afshar Ghahremani, Abbas Raisi, Sohrab Minaei Beirami, Houman Kahroba, Mahnaz Mardani, Omid Dezfoulian, Vahideh Tarhriz","doi":"10.30466/vrf.2024.2018359.4107","DOIUrl":"10.30466/vrf.2024.2018359.4107","url":null,"abstract":"<p><p>Curcumin has been employed in traditional medicine for over a millennium to treat various ailments, and its global use is now widespread. Chinese medicine relies heavily on curcumin as a primary element and uses it to cure infectious diseases, skin disorders, depression, and stress. It has cardioprotective, neuroprotective, and anti-diabetic properties, as well as pharmacological effects on disorders like type II diabetes, atherosclerosis, and human immunodeficiency virus replication. The anti-cancer activity of curcumin has been studied extensively with notable improvements in gastrointestinal, melanoma, urogenital, breast, and lung malignancies. We investigated the anti-inflammatory effects of curcumin on expression of <i>tumor necrosis factor</i> (<i>TNF</i>)<i>-α</i>, <i>c-Fos</i>, and <i>interleukin</i> (<i>IL</i>)<i>-6</i> genes in brain and liver tissue owing to the effects of ketamine anesthesia on postnatal rats. The thalamic and hepatic tissues were collected without anesthesia, immediately after anesthesia, and 4 and 12 hr after anesthesia in control and curcumin treated postnatal rats. The results showed that glucose, triglyceride, high- and low-density lipoprotein levels were lowered with curcumin treatment. We also found that ketamine increased <i>c</i>-<i>Fos</i> and inflammatory cytokines like <i>TNF-α</i> and <i>IL-6</i>, all of which contribute to inflammation. Brain and liver immunohistochemistry studies confirmed the real-time polymerase chain reaction findings. Curcumin injections alone may be effective in decreasing ketamine-induced inflammation in both brain and liver tissues.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"473-480"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-07-15DOI: 10.30466/vrf.2024.2000236.3852
Abolfazl Parvizi Alan, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh
{"title":"Epigallocatechin-3-gallate affects the quality of fresh and frozen-thawed semen of Simmental bull by two different cryopreservation methods.","authors":"Abolfazl Parvizi Alan, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh","doi":"10.30466/vrf.2024.2000236.3852","DOIUrl":"https://doi.org/10.30466/vrf.2024.2000236.3852","url":null,"abstract":"<p><p>During the freezing process of semen, due to the generating of significant amounts of free radicals, the quality of sperm changes. Epigallocatechin-3-gallate (EGCG) is a green tea catechin, which in this study was applied to investigate its effect on the quality of bulls' sperm. We collected semen samples with an artificial vagina from 12 Simmental bulls to evaluate the effect of EGCG (10.00 and 20.00 µmol) in two cryopreserving methods on the quality parameters of semen. We designed six groups including two control groups (method one and two) and four treatments (EGCG 10.00 µmol + method one; EGCG 20.00 µmol + method one; EGCG 10.00 µmol + method two; EGCG 20.00 µmol + method two). The 20.00 µmol EGCG and a method two significantly affected the amending oxidative conditions as well as an increase in total antioxidant capacity and a decrease in malondialdehyde. The effect of EGCG in both concentrations was more on method two. The desired impact on sperm motility, viability, inhibition of lipid peroxidation and sperm DNA damage was observed in EGCG groups compared to control groups. Among the two methods, the method two had fewer adverse effects on the plasma membrane, motility parameters, viability and DNA of sperm. The EGCG in the semen extender yielded a favorable impact on thawed sperm. This effect was prompted in combination with the method two.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"369-377"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-07-15DOI: 10.30466/vrf.2024.2016008.4070
Van Hanh Nguyen, Thi Huong-Giang Tran, Hong Thi Nguyen, Hiep Thi Nguyen, Nhung Thi Nguyen, Tien Phat Do
{"title":"Evaluating potential of H'mong chicken bone marrow-derived mesenchymal stem cells at different ages for primordial germ cells' feeder layer.","authors":"Van Hanh Nguyen, Thi Huong-Giang Tran, Hong Thi Nguyen, Hiep Thi Nguyen, Nhung Thi Nguyen, Tien Phat Do","doi":"10.30466/vrf.2024.2016008.4070","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016008.4070","url":null,"abstract":"<p><p>Primordial germ cells (PGCs) have potential applications in genetic conservation, vaccination, tissue repair therapies, and genetic research. Chicken bone marrow-derived mesenchymal stem cells (cbMSCs) is a good candidate for co-culture with PGCs. However, there is no consensus on the optimal age of donors. In this study, we aimed to compare specific parameters of H'Mong cbMSCs obtained from day 14<sup>th</sup> and 19<sup>th</sup> embryos, and day 3<sup>rd</sup> newborns. Isolated cbMSCs showed characteristics of MSCs. Cells had fibroblast-like morphology, plastic-adherent, expressed specific markers of MSCs and multilineage differentiation potential. The growth rate of cells from day 19<sup>th</sup> embryos was higher than from other ages. Moreover, cells expressed markers of pluripotency such as <i>Nanog</i>, <i>PouV</i>, <i>Sox2</i>, <i>CVH</i>, <i>DAZL</i>, and <i>KIT</i>, known for their role in maintaining stem cell self-renewal and pluripotency. As feeder cells, cbMSCs from three different ages promoted proliferation of H'Mong PGCs during co-culture. These results suggested that cbMSCs from different ages can be used for co-culture H'Mong PGCs which were further used for genetic preservation of H'Mong chicken or gene editing research.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"335-342"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation and evaluation of controlled released implant containing mesoporous selenium nanoparticles loaded with curcumin in rats with spinal cord injury.","authors":"Ehsan Lajmiri, Moosa Javdani, Pegah Khosravian, Mohammad Hashemnia, Hossein Kazemi Mehrjerdi","doi":"10.30466/vrf.2024.2014162.4040","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014162.4040","url":null,"abstract":"<p><p>In this study, a controlled released delivery drug system designed and synthesized by loading curcumin and selenium nanoparticles (SeNaPs) on chitosan hydrogel, and while evaluating the physicochemical properties of the prepared drug delivery system, the tissue changes caused by the local implant of that system in rats with experimental spinal cord injury (SCI) were investigated. For this purpose, 100 adult female rats were randomly divided into five equal groups which are: Control group without any treatment for SCI, chitosan group that received chitosan hydrogel, curcumin group that received curcumin-loaded hydrogel, SeNaP group that received chitosan loaded with SeNaPs and SeNPCur group that received chitosan loaded with SeNaPs and curcumin. On the 3<sup>rd</sup> and 7<sup>th</sup> days of the study, severe infiltration of leukocytes, especially lymphocytes, as well as axon swelling and hemorrhagic necrosis at the lesion sites were observed in all groups, especially the control group. On the 7<sup>th</sup> day, the severity of these injuries decreased in the SeNPCur group and the highest number of astrocytes was observed in this group. In addition, on the 14<sup>th</sup> and 21<sup>st</sup> days of the study, the lowest severity of nerve tissue damage and the lowest presence of inflammatory cells along with the highest number of astrocytes were seen in the SeNPCur group. The glial fibrillary acidic protein study also confirmed the presence of more and significant astrocytes in the SeNPCur, curcumin and SeNP groups at different times of the study, respectively. The histopathological results showed the neuroprotective effects of chitosan hydrogel loaded with selenium and curcumin.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"357-367"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-08-15DOI: 10.30466/vrf.2024.2019127.4115
Hina Ashraf Waiz, Lokesh Gautam
{"title":"Effect of wet feeding on performance, blood plasma indices, intestinal histomorphometry, and economics of broiler chickens under hot climatic conditions.","authors":"Hina Ashraf Waiz, Lokesh Gautam","doi":"10.30466/vrf.2024.2019127.4115","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019127.4115","url":null,"abstract":"<p><p>The study aimed to investigate the impact of wet feeding on broiler chicken performance and intestinal histomorphometry. The research was conducted at the Veterinary College, Udaipur, India, using 144 commercial broiler chicks from day 1 to 6 weeks of age. The chicks were divided randomly into three experimental groups, each with four replicates comprising 48 birds. The control group (G1) was fed dry; while, group 2 (G2) received wet feed supplemented with 1.00 g water <i>per</i> g of feed and group 3 (G3) received wet feed supplemented with 1.20 g water <i>per</i> g of feed. Results revealed that G3 had significantly superior feed conversion ratios, lower feed intake, and increased body weight and weight gain compared to G2 and G1. Among the three treatment groups, there were no variations in blood plasma indicators. In comparison with G1, duodenum and ileum in G2 had higher villus height. The G3 revealed higher net profit margins and 18.89% saving of feed compared to G1, suggesting that it may be a valuable tool for lowering chicken feed costs.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"391-396"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}