Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt.

IF 0.8 4区农林科学 Q3 ZOOLOGY

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI:10.30466/vrf.2023.2007392.3946

Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey

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Abstract

Staphylococcus aureus is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of S. aureus in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for S. aureus using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of S. aureus isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for S. aureus. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (mecA), β-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDRS aureus as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant S. aureus isolates.

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从埃及驼奶中分离出的金黄色葡萄球菌抗菌药耐药性基因的分子特征。

金黄色葡萄球菌是全球最常见的乳腺炎病因之一。本研究旨在确定从埃及马特鲁省骆驼养殖场采集的驼奶样本中金黄色葡萄球菌的流行率和抗菌药耐药性（AMR）模式。采用传统培养法和分子法对 200 份骆驼乳样品进行了金黄色葡萄球菌评估。金黄色葡萄球菌分离物的抗生素敏感性检测采用了盘扩散法和琼脂稀释法，抗生素耐药基因则通过聚合酶链式反应与特定引物进行鉴定。在检测的样本中，有 60 个（30.00%）金黄色葡萄球菌呈阳性。分离菌株对哌拉西林-他唑巴坦的耐药性最高（55.00%），其次是三甲氧苄青霉素-磺胺甲噁唑（45.00%）和阿莫西林（40.00%）。一半的分离菌株具有多重耐药性（MDR）。耐甲氧西林基因（mecA）、β-内酰胺酶基因（blaZ）、四环素耐药基因（tetK）、红霉素耐药基因（ermB）和万古霉素耐药基因（vanA）的检出率分别为 100%、100%、95.00%、90.00% 和 20.00%。总之，金黄色葡萄球菌（MDRS aureus）是临床骆驼乳腺炎的病因之一，是兽医和公共卫生的重大问题。这些发现凸显了埃及骆驼养殖场正确使用抗生素的重要性，以及采用分子技术全面了解耐抗菌素金黄色葡萄球菌分离物遗传特征的必要性。

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来源期刊

Veterinary Research Forum Veterinary-General Veterinary

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.