Veterinary Research Forum最新文献_第4页

Radiological examination of thorax in Awassi sheep. 阿瓦西羊胸部放射学检查。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2025.2026001.4230

Ali Koçyiğit, İsmail Demircioğlu, Gülşah Güngören, Funda Aksünger Karaavci

{"title":"Radiological examination of thorax in Awassi sheep.","authors":"Ali Koçyiğit, İsmail Demircioğlu, Gülşah Güngören, Funda Aksünger Karaavci","doi":"10.30466/vrf.2025.2026001.4230","DOIUrl":"10.30466/vrf.2025.2026001.4230","url":null,"abstract":"Medical imaging methods (magnetic resonance imaging, computed tomography, X-ray, and ultrasound) are used to guide physicians when diagnosing diseases and planning their treatment. Thorax radiography is frequently preferred for the detection of diseases related to the respiratory system and the heart. When the thorax anatomy is known, this facilitates the detection of diseases in these regions. The vertebral heart score (VHS) is a parameter used for the detection of cardiac anomalies. In cases such as cardiomyopathy and chronic pneumonia, the heart silhouette changes and cardiac enlargement is ensued. Knowing the normal VHS parameter is important in the detection of anomalies. In the present study, 15 male and 30 female adult Awassi sheep were used. The thorax region was imaged with an X-ray device in the right lateral position. Each animal was measured for nine parameters in the region. In the measurements, the female and male VHS scores were found to be 9.77 ± 0.48 and 9.69 ± 0.52, respectively, using one method and 10.11 ± 1.64 and 9.96 ± 0.73, respectively, using the other method. No statistical difference was found between the two methods. The cardiodiaphragmatic contact parameter was observed to be statistically significant between males and females. No statistical difference was found in other parameters. Consequently, this study was carried out to determine the VHS values in Awassi sheep, and the thorax measurements were evaluated. This study is considered to contribute to veterinary surgeons and the field of veterinary surgery.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"387-390"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of quercetin liposome on acute low dose diazinon-induced oxidative stress and neurobehavioral disorders by affecting serotonin metabolite in mature male rats. 槲皮素脂质体通过影响成熟雄性大鼠血清素代谢物对急性低剂量二嗪嗪诱导的氧化应激和神经行为障碍的保护作用。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2032844.4326

Beheshteh Babazadeh, Homeira Hatami Nemati, Nasser Arsalani, Gholamreza Dehghan, Sama Radbin, Valida Madatova

{"title":"Protective effect of quercetin liposome on acute low dose diazinon-induced oxidative stress and neurobehavioral disorders by affecting serotonin metabolite in mature male rats.","authors":"Beheshteh Babazadeh, Homeira Hatami Nemati, Nasser Arsalani, Gholamreza Dehghan, Sama Radbin, Valida Madatova","doi":"10.30466/vrf.2024.2032844.4326","DOIUrl":"10.30466/vrf.2024.2032844.4326","url":null,"abstract":"Diazinon (DZN) is a widely used organophosphate. We studied the effect of quercetin pegylated liposome (QPEGL) on acute low dose DZN-induced oxidative stress and behavioral disorders through monitoring brain serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) in mature male rats. Animals were treated in two control groups that received a single dose of normal saline and dimethyl sulfoxide, and four groups that received a single dose of DZN 10.00 mg kg-1 (DZN), DZN 10.00 mg kg + quercetin 20.00 mg kg-1, DZN 10.00 mg kg-1 + PEGL 20.00 mg kg-1, DZN 10.00 mg kg-1 + QPEGL 20.00 mg kg-1 (QPEGL), respectively. Performances of the rats were investigated by the open field and elevated plus maze tests. Twenty-four hr after the treatments, animals' brains were harvested and frozen at - 80.00 ˚C. Brain tissues 5-HIAA level was determined by the enzyme-linked immunosorbent assay. Furthermore, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were determined for oxidative stress analysis. The motor activity was significantly reduced in the DZN group compared to the control group following increased anxiety-like behavior and ameliorated by QPEGL. Moreover, 5-HIAA and MDA levels notably increased in the DZN group compared to the control group and significantly decreased in the QPEGL group compared to the DZN group. The SOD and GPx contents were not significantly changed in the DZN group compared to the control; although, these parameters improved after treatment with QPEGL. Acute low dose DZN exposure resulted in lipid peroxidation and elevated levels of the serotonin metabolite (5-HIAA), leading to neurobehavioral disorders, such as anxiety-like behavior and impaired motor activity, which were alleviated by QPEGL.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"399-406"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal parasites in shelter dogs in Khorasan Razavi province, Iran: prevalence and mini review in Iran and some neighboring countries. 伊朗呼罗珊拉扎维省收容犬的胃肠道寄生虫：伊朗和一些邻国的流行情况和初步审查。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2024.2037586.4391

Amin Bakhshani, Gholamreza Razmi

{"title":"Gastrointestinal parasites in shelter dogs in Khorasan Razavi province, Iran: prevalence and mini review in Iran and some neighboring countries.","authors":"Amin Bakhshani, Gholamreza Razmi","doi":"10.30466/vrf.2024.2037586.4391","DOIUrl":"10.30466/vrf.2024.2037586.4391","url":null,"abstract":"Dogs serve as definitive or intermediate hosts for some protozoan and helminth parasites, some of which are of zoonotic, and others cause severe infectious diseases in other animals. The aim of this study was to determine the prevalence of intestinal parasites in shelter dogs in Khorasan Razavi province, Iran. A total of 323 fecal samples were randomly collected from shelter dogs in Mashhad and Neyshabur areas. All samples were then transferred to the Parasitology Laboratory and examined detect intestinal protozoa and helminths using the formalin-ether technique. Out of the 323 fecal samples, 92 (28.48%) tested positive for intestinal parasites. The overall prevalence of intestinal parasites in shelter dogs in Mashhad and Neyshabur areas were 23.83 and 35.38%, respectively. Monospecific infection was found in 19.50% of dogs, while concurrent infection with two or more species was seen in 8.97% of dogs. The frequencies of the nine detected parasites were as follows: Sarcocystis sp. 12.38%, Giardia sp. 4.64%, Cystoisospora sp. 2.78%, Hammondia Neospora-like oocysts 2.16%, Taenia sp. 6.19%, Toxascaris leonina 7.43%, Dicrocoelium dendriticum 1.54%, Toxocara canis 0.92% and Capillaria sp. 0.30%. The results of this study showed the high frequency of intestinal parasites in shelter dogs. To control important zoonotic helminthic diseases such as echinococcosis and toxocariasis, it is recommended to eliminate stray dogs in cooperation with municipalities and veterinary departments in Iran. If stray dogs are transferred to shelters, they should receive regular treatment with effective anti-helminthic drugs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"447-454"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of olive oil against cardiac aging through mitophagy and apoptosis. 橄榄油通过有丝分裂和细胞凋亡对心脏衰老的保护作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030624.4304

Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari

{"title":"Protective effects of olive oil against cardiac aging through mitophagy and apoptosis.","authors":"Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari","doi":"10.30466/vrf.2024.2030624.4304","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030624.4304","url":null,"abstract":"Cardiac mitochondrial dysfunction is an important feature of aged heart. However, there is still no potent agent to ameliorate cardiac function abnormalities in aged hosts. Olive oil (OLO), containing monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-diabetic, anti-inflammatory, and anti-hypertensive effects. We evaluated the beneficial impacts of OLO against aging-related cardiac dysfunction. Wistar rats were randomly allotted into three groups with eight rats, including control, aged rats receiving D-galactose (D-GAL), and aged rats administrated with D-galactose plus OLO (D-GAL + OLO). Aged animals were received D-GAL at a dose of 150.00 mg kg-1 daily through intra-peritoneal injection for aging induction. The animals in D-GAL + OLO group were co-administrated with oral OLO at a dose of 1.00 mL kg-1 by gavage feeding daily. The administration term was eight weeks. A histological examination of heart tissue was performed. The heart tissues were also harvested to assay the oxidative stress and molecular parameters. The aged animals showed cardiac hypertrophy, increased malondialdehyde level and Bax expression, and reduced mitofusin 2, phosphatase and tensin homologue-induced putative kinase 1, dynamin-related protein 1, and Bcl2 expressions in comparison with the control animals. The OLO treatment ameliorated all these parameters. Overall, OLO could improve cardiac aging through reducing oxidative stress, enhancing genes mediated mitophagy, and improving genes mediated apoptosis in the heart.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"27-33"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination and comparison of volatile compounds of different poultry species eggs. 不同禽种鸡蛋挥发性成分的测定与比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2022511.4164

Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz

{"title":"Determination and comparison of volatile compounds of different poultry species eggs.","authors":"Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz","doi":"10.30466/vrf.2024.2022511.4164","DOIUrl":"10.30466/vrf.2024.2022511.4164","url":null,"abstract":"All fresh poultry eggs differ greatly in chemical components. Many chemicals have been investigated in this field, but the presence of differences in odor components between species has not yet been investigated. For this reason, this study aimed to determine the volatile compounds of the eight different poultry species eggs and the differences among them. In this context, 180 eggs (20 from each species) were purchased from different farms, and the volatile compounds of raw eggs were determined by the solid phase micro-extraction method. Following the analysis, 41 volatile compounds and 12 organic groups were identified. The variety and ratios of these compounds varied among the species, with pigeon eggs containing the greatest number of compounds (19 compounds) and quail, partridge, and pheasant eggs containing the lowest numbers (seven compounds). Acetamide, 2-fluoro-, and D-limonene compounds were found in every species of eggs. The other species eggs did not contain any of the 10 volatile chemicals detected in pigeon eggs, eight in chicken eggs, and one in quail eggs. It was discovered that the eggs volatile compounds counts and chemical profiles varied, indicating differences in their tastes, smells, and aromas. The volatile chemicals found in this study may be species-specific and can be used as indicators to identify which eggs belong to which species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"149-159"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model. 局部给药氨甲环酸预防术后硬膜外纤维化：来自兔椎板切除术模型的见解。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2031220.4312

Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem

{"title":"Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model.","authors":"Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem","doi":"10.30466/vrf.2024.2031220.4312","DOIUrl":"10.30466/vrf.2024.2031220.4312","url":null,"abstract":"Significant advancements in imaging and surgical methodologies have led to more frequent performance of neurosurgical procedures such as laminectomy in both animal and human patients. Epidural fibrosis (EF) is defined as the excessive formation of scar tissue in the epidural space after lumbar laminectomy, often resulting in recurring postoperative pain. Given the association between postoperative hematoma accumulation at the laminectomy site and the development of EF, the present study aimed to evaluate the preventive impact of tranexamic acid (TXA), an antifibrinolytic agent with well-recognized hemostatic properties across various surgical fields. A rabbit laminectomy model was constructed to assess its effectiveness in reducing EF formation. A total number of 18 adult New Zealand White male rabbits were randomly divided into two groups: The control (saline) group and the treatment (topical TXA) group. Each rabbit underwent a two-level laminectomy at L3-L4. The treatment group received 5.00 mL of 100 mg mL-1 TXA solution applied topically to the laminectomy site, while the control group received 5.00 mL of saline. Postoperative evaluations included magnetic resonance imaging at week six to assess EF, followed by histopathological examinations to evaluate fibroblast cell density in scar tissue, EF grading and thickness of the dura mater. The analysis of magnetic resonance imaging and histopathologic data revealed significant differences between the two groups indicating that topical administration of TXA might be a promising approach for preventing EF.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"181-187"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology. 揭示多酶鸡尾酒索诺芽孢杆菌BD92对商品肉鸡生长性能和肠道组织学的有益影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2025777.4221

Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir

{"title":"Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology.","authors":"Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir","doi":"10.30466/vrf.2024.2025777.4221","DOIUrl":"10.30466/vrf.2024.2025777.4221","url":null,"abstract":"Crude fiber (CF) is a vital component in poultry nutrition with a notable phytonutrient effectively indicating the presence of indigestible biomass in food due to the absence of digestive enzymes for CF in broilers. This study aimed to analyze the properties of a multi-enzyme cocktail (MEC) Bacillus sonorensis BD92 (BsBD92) comprised of xylanase, β-glucosidase, exo-glucanase, and endo-glucanase enzymes. Also, this study intended to look at the growth performance and intestinal histology of broilers in the starter and finisher phases by the addition of MEC BsBD92 to their diet. To evaluate the efficacy of MEC BsBD92, 140 one-day-old unsexed Cobb500 broiler chicks were randomly divided into seven groups receiving different diets. The characterization of exo-glucanase, xylanase, β-glucosidase, and endo-glucanase showed that their peak activities were observed at a temperature of 50.00 ˚C and a pH of 5.50. The 6.00% CF and 2.00 X MEC BsBD92 improved the intestinal morphology and feed conversion ratio, demonstrating a synergistic effect on growth performance. Whereas, increasing meat percentages to 61.06 and 65.09 g per 100 g body weight during the starter and finisher phases was also observed, respectively. The lipid profiles revealed significant variations in triglyceride and cholesterol levels. This study provides an innovative approach, considering not only lowering the feed cost using inexpensive fibrous feedstuffs but also improving the feed efficiency through supplementation of MEC BsBD92.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"209-219"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the efficacies of Rhodococcus equi recombinant vaccine in mice. 马属红球菌重组疫苗对小鼠免疫效果的比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2028438.4266

Gokcenur Sanioglu Golen, Osman Erganiş, Aslı Balevi

{"title":"Comparison of the efficacies of Rhodococcus equi recombinant vaccine in mice.","authors":"Gokcenur Sanioglu Golen, Osman Erganiş, Aslı Balevi","doi":"10.30466/vrf.2024.2028438.4266","DOIUrl":"10.30466/vrf.2024.2028438.4266","url":null,"abstract":"Rhodococcus equi is an important bacterial pathogen and causes severe chronic granulomatous pneumonia in foals below 6 months of age. It has also become an opportunistic and emerging pathogen in immunocompromised humans. Vaccination is the most cost-effective strategy for controlling and preventing this infection. Although several potential virulence genes and candidate immunogens have been identified over the years, no effective vaccine is currently available to prevent R. equi disease in horses. Recently, bacterial vector vaccines have been shown to be promising for R. equi. In this study, the virulence-associated protein A (VapA) gene of R. equi was cloned into Protein Expression System small ubiquitin-related modifier (pET-SUMO) expression vectors and transferred into Escherichia coli BL21 (DE3). Also, adjuvant significantly affects the efficacy of recombinant vaccines. Therefore, native VapA and recombinant VapA were formulated with Immunostimuling Microparticle System (IMS 3012) or PetGel A (recommended for horses) and subcutaneously administered to mice. The immunization effect of four different vaccines was determined by assaying antibody titers and survival rates. The antibody response was slightly higher in the PetGel A formulations than IMS 3012. Survival rates were lower in the PetGel A formulations than IMS 3012. Given these results, recombinant VapA adjuvanted with PetGel A represents a promising formulation for developing new-generation R. equi vaccines.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"253-259"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic resistance dynamics of some common probiotic Lactobacillus species and avian pathogenic Escherichia coli in colibacillosis-diseased versus healthy broiler chickens. 几种常见益生菌乳酸菌和禽致病性大肠杆菌在大肠杆菌病与健康肉鸡中的耐药性动态。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2025.2037056.4381

Nimra Khalid, Syed Mohsin Bukhari, Waqas Ali, Ali Ahmad Sheikh

{"title":"Antibiotic resistance dynamics of some common probiotic Lactobacillus species and avian pathogenic Escherichia coli in colibacillosis-diseased versus healthy broiler chickens.","authors":"Nimra Khalid, Syed Mohsin Bukhari, Waqas Ali, Ali Ahmad Sheikh","doi":"10.30466/vrf.2025.2037056.4381","DOIUrl":"10.30466/vrf.2025.2037056.4381","url":null,"abstract":"This study focuses on understanding how the sensitivity patterns of common probiotic Lactobacillus species differ between healthy and colibacillosis-diseased broiler chickens and explores any correlation between these patterns and avian pathogenic Escherichia coli (APEC). Ileum samples were collected from the healthy (n = 10) and colibacillosis-diseased (n = 10) broiler poultry farms. Lactobacillus and E. coli were identified and incidence rate of six probiotic Lactobacillus species (L. acidophilus, L. brevis, L. casei, L. plantrum, L. delbrueckii, and L. fermenti) was determined, and their susceptibility to different antibiotics (ampicillin, chloramphenicol, erythromycin, kanamycin, tetracycline, and vancomycin) was tested. A total of 120 Lactobacilli were examined. Certain Lactobacillus spp. from healthy birds (L. brevis) and those affected by colibacillosis (L. acidophilus) showed better sensitivity and lower multiple antibiotic resistance to chloramphenicol, erythromycin, and kanamycin. Statistical analysis revealed significant correlations between ampicillin and chloramphenicol, ampicillin and erythromycin, ampicillin and tetracycline, and ampicillin and vancomycin resistance. Also, a significant correlation was observed between erythromycin and tetracycline, and kanamycin and vancomycin. All APEC strains showed multiple antibiotic resistance. A strong positive correlation was found between resistant Lactobacillus spp. and APEC isolates from diseased birds, indicating co-occurrence of antibiotic resistance. The study suggests that antibiotic resistance in APEC and Lactobacillus species may spread through horizontal transfer, highlighting the need for improved anti-microbial management in poultry systems.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"391-397"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases. N- 乙酰半胱氨酸在治疗犬血液原虫病引起的全血细胞减少症中可增强骨髓活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2033155.4328

Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das

{"title":"N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases.","authors":"Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das","doi":"10.30466/vrf.2024.2033155.4328","DOIUrl":"https://doi.org/10.30466/vrf.2024.2033155.4328","url":null,"abstract":"Canine hemoprotozoan diseases viz. ehrlichiosis and babesiosis are mostly associated with critical anemia and thrombocytopenia with pancytopenic changes, leading to multi-organ failure. For faster recovery of patients with complicated hemoprotozoan diseases, whole blood transfusion or bone marrow stimulating agents to produce more red blood cells (RBCs) and platelets might be helpful. Unfortunately, canine specific transfusion procedures are expensive and even not available in many developing countries. Development of alternate therapeutic modality by bone marrow stimulation to augment the production of RBCs and platelets and thus, to treat the critical pancytopenic patients is and an urgent necessity. N-acetylcysteine (NAC), acts as a precursor of reduced glutathione and increases the production of bone marrow B cells. It also improves viability and self-renewal capacity of stem cells and thus, boosts hematopoietic differentiation by protecting induced pluripotent stem cells. This study envisaged to develop alternate therapeutic approach to combat pancytopenia secondary to canine hemoprotozoan diseases. Bone marrow mediated aplastic pancytopenia was induced experimentally by administration of cyclophosphamide in rats. Bone marrow stimulating property of NAC was compared with desmopressin, another bone marrow stimulator, which revealed better in terms of hematobiochemical and histopathological changes. Results of rat model study were extrapolated in clinical canine hemoprotozoan cases having pancytopenia. Dogs treated with hemoprotozoan disease specific therapy along with NAC rendered favorable changes by haltering the progression of critical anemia and thrombocytopenia. Study revealed that supplementation of NAC along with canine hemoprotozoan specific therapy is beneficial to alleviate pancytopenia.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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