Veterinary Research Forum最新文献_第4页

Evaluating potential of H'mong chicken bone marrow-derived mesenchymal stem cells at different ages for primordial germ cells' feeder layer. 评估不同年龄的蒙鸡骨髓间充质干细胞作为原始生殖细胞哺育层的潜力。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2016008.4070

Van Hanh Nguyen, Thi Huong-Giang Tran, Hong Thi Nguyen, Hiep Thi Nguyen, Nhung Thi Nguyen, Tien Phat Do

{"title":"Evaluating potential of H'mong chicken bone marrow-derived mesenchymal stem cells at different ages for primordial germ cells' feeder layer.","authors":"Van Hanh Nguyen, Thi Huong-Giang Tran, Hong Thi Nguyen, Hiep Thi Nguyen, Nhung Thi Nguyen, Tien Phat Do","doi":"10.30466/vrf.2024.2016008.4070","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016008.4070","url":null,"abstract":"Primordial germ cells (PGCs) have potential applications in genetic conservation, vaccination, tissue repair therapies, and genetic research. Chicken bone marrow-derived mesenchymal stem cells (cbMSCs) is a good candidate for co-culture with PGCs. However, there is no consensus on the optimal age of donors. In this study, we aimed to compare specific parameters of H'Mong cbMSCs obtained from day 14th and 19th embryos, and day 3rd newborns. Isolated cbMSCs showed characteristics of MSCs. Cells had fibroblast-like morphology, plastic-adherent, expressed specific markers of MSCs and multilineage differentiation potential. The growth rate of cells from day 19th embryos was higher than from other ages. Moreover, cells expressed markers of pluripotency such as Nanog, PouV, Sox2, CVH, DAZL, and KIT, known for their role in maintaining stem cell self-renewal and pluripotency. As feeder cells, cbMSCs from three different ages promoted proliferation of H'Mong PGCs during co-culture. These results suggested that cbMSCs from different ages can be used for co-culture H'Mong PGCs which were further used for genetic preservation of H'Mong chicken or gene editing research.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"335-342"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and evaluation of controlled released implant containing mesoporous selenium nanoparticles loaded with curcumin in rats with spinal cord injury. 制备含有姜黄素的介孔硒纳米颗粒的控释植入物并对其在脊髓损伤大鼠中的应用进行评估。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2014162.4040

Ehsan Lajmiri, Moosa Javdani, Pegah Khosravian, Mohammad Hashemnia, Hossein Kazemi Mehrjerdi

{"title":"Preparation and evaluation of controlled released implant containing mesoporous selenium nanoparticles loaded with curcumin in rats with spinal cord injury.","authors":"Ehsan Lajmiri, Moosa Javdani, Pegah Khosravian, Mohammad Hashemnia, Hossein Kazemi Mehrjerdi","doi":"10.30466/vrf.2024.2014162.4040","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014162.4040","url":null,"abstract":"In this study, a controlled released delivery drug system designed and synthesized by loading curcumin and selenium nanoparticles (SeNaPs) on chitosan hydrogel, and while evaluating the physicochemical properties of the prepared drug delivery system, the tissue changes caused by the local implant of that system in rats with experimental spinal cord injury (SCI) were investigated. For this purpose, 100 adult female rats were randomly divided into five equal groups which are: Control group without any treatment for SCI, chitosan group that received chitosan hydrogel, curcumin group that received curcumin-loaded hydrogel, SeNaP group that received chitosan loaded with SeNaPs and SeNPCur group that received chitosan loaded with SeNaPs and curcumin. On the 3rd and 7th days of the study, severe infiltration of leukocytes, especially lymphocytes, as well as axon swelling and hemorrhagic necrosis at the lesion sites were observed in all groups, especially the control group. On the 7th day, the severity of these injuries decreased in the SeNPCur group and the highest number of astrocytes was observed in this group. In addition, on the 14th and 21st days of the study, the lowest severity of nerve tissue damage and the lowest presence of inflammatory cells along with the highest number of astrocytes were seen in the SeNPCur group. The glial fibrillary acidic protein study also confirmed the presence of more and significant astrocytes in the SeNPCur, curcumin and SeNP groups at different times of the study, respectively. The histopathological results showed the neuroprotective effects of chitosan hydrogel loaded with selenium and curcumin.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"357-367"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of wet feeding on performance, blood plasma indices, intestinal histomorphometry, and economics of broiler chickens under hot climatic conditions. 湿饲对炎热气候条件下肉鸡生产性能、血浆指数、肠道组织形态学和经济效益的影响

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2019127.4115

Hina Ashraf Waiz, Lokesh Gautam

{"title":"Effect of wet feeding on performance, blood plasma indices, intestinal histomorphometry, and economics of broiler chickens under hot climatic conditions.","authors":"Hina Ashraf Waiz, Lokesh Gautam","doi":"10.30466/vrf.2024.2019127.4115","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019127.4115","url":null,"abstract":"The study aimed to investigate the impact of wet feeding on broiler chicken performance and intestinal histomorphometry. The research was conducted at the Veterinary College, Udaipur, India, using 144 commercial broiler chicks from day 1 to 6 weeks of age. The chicks were divided randomly into three experimental groups, each with four replicates comprising 48 birds. The control group (G1) was fed dry; while, group 2 (G2) received wet feed supplemented with 1.00 g water per g of feed and group 3 (G3) received wet feed supplemented with 1.20 g water per g of feed. Results revealed that G3 had significantly superior feed conversion ratios, lower feed intake, and increased body weight and weight gain compared to G2 and G1. Among the three treatment groups, there were no variations in blood plasma indicators. In comparison with G1, duodenum and ileum in G2 had higher villus height. The G3 revealed higher net profit margins and 18.89% saving of feed compared to G1, suggesting that it may be a valuable tool for lowering chicken feed costs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"391-396"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of curcumin-loaded polycaprolactone scaffold on Achilles tendon repair in rats.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2029563.4286

Narges Sufian, Mehdi Behfar, Rahim Hobbenaghi, Siamak Asri-Rezaei

{"title":"Effect of curcumin-loaded polycaprolactone scaffold on Achilles tendon repair in rats.","authors":"Narges Sufian, Mehdi Behfar, Rahim Hobbenaghi, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2029563.4286","DOIUrl":"10.30466/vrf.2024.2029563.4286","url":null,"abstract":"Scaffolds play a crucial role in tendon healing by providing structural support, promoting cell infiltration, and guiding tissue regeneration. Polycaprolactone (PCL) has been used as a polymer in biological scaffolds for several tissue engineering studies. This study aimed to investigate the effects of curcumin-loaded PCL scaffold on Achilles tendon using a tenotomy model in rats. Twenty adult male Wistar rats were randomized into two groups. In control group, tenotomy and suture placement were performed. The identical intervention followed by the implantation of curcumin-loaded PCL scaffold around the tendon stumps was performed in the treatment group. The nanofibrous PCL scaffold containing 5.00% curcumin was fabricated by electrospinning. Walking track analysis was performed weekly. Then, after 6 weeks, histopathological examination and tendon mechanical tests were performed. The weekly walking track analysis revealed a significant improvement in Achilles functional index in scaffold-treated rats from week three to six. The rate of functional improvement was remarkably slower in the control group. Histopathological examination revealed aseptic inflammation and enhanced neovascularization in the treatment group. Also, collagen arrangement and density were significantly improved in this group compared to the control samples including less regular orientation and loose organization of collagen fibers. Significant increase in mechanical properties, except for strain, was observed in the treatment group. The present study demonstrated that implantation of curcumin-loaded PCL scaffold resulted in increased fibrillar architecture, as well as improved mechanical properties and Achilles functional index in rats. To reduce the biodegradation-induced inflammation, an anti-inflammatory treatment is recommended.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"621-627"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic resistance patterns, characteristics of virulence and resistance genes and genotypic analysis of Salmonella serotypes recovered from different sources. 从不同来源回收的沙门氏菌血清型的抗生素耐药性模式、毒力和耐药性基因的特征以及基因型分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2019507.4123

Seyed Mahdi Hosseini, Rahem Khoshbakht, Hami Kaboosi, Fatemeh Peyravii Ghadikolaii

{"title":"Antibiotic resistance patterns, characteristics of virulence and resistance genes and genotypic analysis of Salmonella serotypes recovered from different sources.","authors":"Seyed Mahdi Hosseini, Rahem Khoshbakht, Hami Kaboosi, Fatemeh Peyravii Ghadikolaii","doi":"10.30466/vrf.2024.2019507.4123","DOIUrl":"10.30466/vrf.2024.2019507.4123","url":null,"abstract":"The present study evaluated the Salmonella isolates obtained from various origins in Iran. Salmonella strains previously recovered and stored in the veterinary microbiology laboratory were serotyped and subjected to antibiotic susceptibility test, detection of the virulence and resistance genes by polymerase chain reaction (PCR), and genotyping by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). All Salmonella isolates showed resistance to erythromycin and the most resistance rates were detected for trimethoprim (86.66%), ampicillin (75.00%), and sulfamethoxazole-trimethoprim (63.33%), respectively. In total, 86.33% of the isolates were known as multi-drug resistant and none of the isolates showed resistance to cefepime, nalidixic acid, imipenem, ceftriaxone, and polymyxin B. The virulence genes, invA, sdiA, and hilA besides the tetA resistance gene were identified in all 60 Salmonella strains. The most prevalent resistance genes were respectively tetC (70.00%), sul2 (58.33%), and ereA (55.00%). Statistical analysis revealed a significant difference between Salmonella serotypes associated with the sul1 resistance gene. In ERIC-PCR analysis, 14 distinct clusters were obtained. Statistically, there were significant relationships between the source and ERIC's genomic pattern and between the serotype of Salmonella isolates and genotypic pattern of ERIC. According to the results, Salmonella serotypes from non-human sources had considerable resistance to different antibiotics and carried significant virulence determinants and resistance genes. In addition, ERIC-PCR showed relevant results in discriminating Salmonella serotypes from other sources.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"499-508"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens. 用不同的组织病理学技术研究肉鸡心包积水综合征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2023088.4174

Hossein Alavizadeh, Zahra Moosavi, Gholamreza Hashemi Tabar

{"title":"Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens.","authors":"Hossein Alavizadeh, Zahra Moosavi, Gholamreza Hashemi Tabar","doi":"10.30466/vrf.2024.2023088.4174","DOIUrl":"10.30466/vrf.2024.2023088.4174","url":null,"abstract":"Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran. The dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia and necrotizing enteritis were some of the findings reported in previous research. Low macrophage infiltration rate and brain lesions were other discoveries in Hematoxylin and Eosin (H&E) examination. Feulgen reaction and Cluster of Differentiation 68 (CD68) immunohistochemical staining were used for a comprehensive investigation and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggested that HPS might cause brain damage and the susceptibility of the Arian breed to the adenovirus needs further investigation.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"559-564"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia. 对传染性山羊胸膜肺炎中肥大细胞、白细胞介素 17 和白细胞介素 1β 特征进行评估的免疫组化研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2023580.4184

Ozhan Karatas, Gokhan Akcakavak

{"title":"An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia.","authors":"Ozhan Karatas, Gokhan Akcakavak","doi":"10.30466/vrf.2024.2023580.4184","DOIUrl":"10.30466/vrf.2024.2023580.4184","url":null,"abstract":"Contagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1β protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immuno-histochemical examination, MC profile and local IL-17 and IL-1β protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1β scores were detected in the peracute-acute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1β expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course. Additionally, MC profile was evaluated for the first time in naturally infected CCPP.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"515-521"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report. 使用卡泊芬净成功治疗白色念珠菌诱发的犬真菌性膀胱炎：病例报告。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2018960.4113

Sei-Myoung Han

{"title":"Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report.","authors":"Sei-Myoung Han","doi":"10.30466/vrf.2024.2018960.4113","DOIUrl":"https://doi.org/10.30466/vrf.2024.2018960.4113","url":null,"abstract":"A 7-year-old castrated male poodle was brought to the referral Animal Medical Center and diagnosed with diabetes and pancreatitis. One month later, the patient presented with cloudy urine, and ultrasonography revealed a large number of spherical substances. The patient was subsequently diagnosed with fungal cystitis with Candida albicans. Initially, 10.00 mg kg-1 itraconazole was prescribed twice daily for six weeks, and the symptoms of prolonged urination improved; however, the fungal balls persisted in the bladder. The six months later, the patient showed recurrent symptoms, such as dysuria and stranguria; therefore, 5.00 mg kg-1 fluconazole was prescribed twice daily; however, it was not effective. Subsequently, 1.00 mg kg-1 caspofungin once daily was administered for three consecutive days. Finally, the fungal balls in the bladder disappeared. The patient was regularly monitored after completion of treatment and, 17 months later, doing well without recurrence. Few reports exist on the use of caspofungin in veterinary medicine. The recommended dose of caspofungin in dogs remains unknown. In the case of azole-resistant Candida, treatment using caspofungin should be considered; although, additional studies on the established dosing and side effects are needed.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"435-438"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial. 在传统牧场系统中对奶牛进行分娩和分娩管理培训的效果：现场试验。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2007348.3945

Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori

{"title":"Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial.","authors":"Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori","doi":"10.30466/vrf.2023.2007348.3945","DOIUrl":"10.30466/vrf.2023.2007348.3945","url":null,"abstract":"The interval between parturition and subsequent pregnancy is called the days open or calving to conception interval and is affected by several factors, especially dystocia. Dystocia is an ab-normal or difficult calving that may require assistance during labor. This study is a field trial in health education and the research team developed a comprehensive training program for farmers to educate them about the normal process of parturition in dairy cows and when and how to assist in parturition or dystocia. A series of classes was held for farmers and the study covered 486 multi-parous dairy cows, with 173 belonging to the group of trained farms (educated farmers) and 313 to the control group (non-educated farmers). Although dystocia was lower in the educated group, there were no significant differences in retained placenta between two groups. However, cows in the educated group had a better conception rate (lower service per conception) in sub-sequent parturitions. Hence, the median number of days open for cows from trained farmers was significantly lower than other farmers (85 days compared to 120 days, respectively). Based on Cox regression analysis, uterine prolapse, retained placenta, and dystocia could significantly impact subsequent pregnancies. Dystocia affects days open, and training on parturition and dystocia management can effectively reduce the numbers of days open in dairy cows.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"139-144"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic potential of autochthonous Lactobacillus species from buffalo calves in controlling multidrug resistant Escherichia coli. 水牛犊自生乳酸杆菌在控制耐多药大肠杆菌方面的益生潜力

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2024.2012559.4024

Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu

{"title":"Probiotic potential of autochthonous Lactobacillus species from buffalo calves in controlling multidrug resistant Escherichia coli.","authors":"Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu","doi":"10.30466/vrf.2024.2012559.4024","DOIUrl":"10.30466/vrf.2024.2012559.4024","url":null,"abstract":"The aim of this study was to investigate the probiotic potential of autochthonous Lactobacillus species isolated from buffalo calves against multidrug-resistant Escherichia coli. A total of 252 rectal swabs were collected from healthy neonatal buffalo calves under 30 days old from six districts of Andhra Pradesh, India in a completely randomized design from August 2019 to August 2021, of which 190 Lactobacillus strains were isolated based on cultural, morphological, biochemical and molecular tests. Out of 190 isolates, 57 showed high levels of auto-aggregation (> 80.00%) and hydrophobicity (> 60.00%) and 51 of the 57 isolates had a zone of inhibition greater than 15.00 mm in diameter against multidrug-resistant E. coli in an Agar well diffusion assay. Among the 51 isolates, 36 were found to be acid and bile tolerant and showed varying levels of sensitivity to antibiotics such as erythromycin, clindamycin, tetracycline, chloramphenicol, and ampicillin. Among the 36 isolates, Limosilactobacillus reuteri 178, L. reuteri 209, L. fermentum 182, L. fermentum 211, and Lactiplanti-bacillus plantarum 34 were non-hemolytic, and none of the isolates were able to hydrolyse gelatine. Therefore, these five autochthonous Lactobacillus species may be used in probiotic or synbiotic formulations against multidrug resistant E. coli in buffalo calves.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"275-282"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0