Veterinary Research Forum最新文献_第3页

Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model. 局部给药氨甲环酸预防术后硬膜外纤维化：来自兔椎板切除术模型的见解。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2031220.4312

Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem

{"title":"Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model.","authors":"Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem","doi":"10.30466/vrf.2024.2031220.4312","DOIUrl":"10.30466/vrf.2024.2031220.4312","url":null,"abstract":"Significant advancements in imaging and surgical methodologies have led to more frequent performance of neurosurgical procedures such as laminectomy in both animal and human patients. Epidural fibrosis (EF) is defined as the excessive formation of scar tissue in the epidural space after lumbar laminectomy, often resulting in recurring postoperative pain. Given the association between postoperative hematoma accumulation at the laminectomy site and the development of EF, the present study aimed to evaluate the preventive impact of tranexamic acid (TXA), an antifibrinolytic agent with well-recognized hemostatic properties across various surgical fields. A rabbit laminectomy model was constructed to assess its effectiveness in reducing EF formation. A total number of 18 adult New Zealand White male rabbits were randomly divided into two groups: The control (saline) group and the treatment (topical TXA) group. Each rabbit underwent a two-level laminectomy at L3-L4. The treatment group received 5.00 mL of 100 mg mL-1 TXA solution applied topically to the laminectomy site, while the control group received 5.00 mL of saline. Postoperative evaluations included magnetic resonance imaging at week six to assess EF, followed by histopathological examinations to evaluate fibroblast cell density in scar tissue, EF grading and thickness of the dura mater. The analysis of magnetic resonance imaging and histopathologic data revealed significant differences between the two groups indicating that topical administration of TXA might be a promising approach for preventing EF.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"181-187"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology. 揭示多酶鸡尾酒索诺芽孢杆菌BD92对商品肉鸡生长性能和肠道组织学的有益影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2025777.4221

Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir

{"title":"Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology.","authors":"Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir","doi":"10.30466/vrf.2024.2025777.4221","DOIUrl":"10.30466/vrf.2024.2025777.4221","url":null,"abstract":"Crude fiber (CF) is a vital component in poultry nutrition with a notable phytonutrient effectively indicating the presence of indigestible biomass in food due to the absence of digestive enzymes for CF in broilers. This study aimed to analyze the properties of a multi-enzyme cocktail (MEC) Bacillus sonorensis BD92 (BsBD92) comprised of xylanase, β-glucosidase, exo-glucanase, and endo-glucanase enzymes. Also, this study intended to look at the growth performance and intestinal histology of broilers in the starter and finisher phases by the addition of MEC BsBD92 to their diet. To evaluate the efficacy of MEC BsBD92, 140 one-day-old unsexed Cobb500 broiler chicks were randomly divided into seven groups receiving different diets. The characterization of exo-glucanase, xylanase, β-glucosidase, and endo-glucanase showed that their peak activities were observed at a temperature of 50.00 ˚C and a pH of 5.50. The 6.00% CF and 2.00 X MEC BsBD92 improved the intestinal morphology and feed conversion ratio, demonstrating a synergistic effect on growth performance. Whereas, increasing meat percentages to 61.06 and 65.09 g per 100 g body weight during the starter and finisher phases was also observed, respectively. The lipid profiles revealed significant variations in triglyceride and cholesterol levels. This study provides an innovative approach, considering not only lowering the feed cost using inexpensive fibrous feedstuffs but also improving the feed efficiency through supplementation of MEC BsBD92.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"209-219"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the efficacies of Rhodococcus equi recombinant vaccine in mice. 马属红球菌重组疫苗对小鼠免疫效果的比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2028438.4266

Gokcenur Sanioglu Golen, Osman Erganiş, Aslı Balevi

{"title":"Comparison of the efficacies of Rhodococcus equi recombinant vaccine in mice.","authors":"Gokcenur Sanioglu Golen, Osman Erganiş, Aslı Balevi","doi":"10.30466/vrf.2024.2028438.4266","DOIUrl":"10.30466/vrf.2024.2028438.4266","url":null,"abstract":"Rhodococcus equi is an important bacterial pathogen and causes severe chronic granulomatous pneumonia in foals below 6 months of age. It has also become an opportunistic and emerging pathogen in immunocompromised humans. Vaccination is the most cost-effective strategy for controlling and preventing this infection. Although several potential virulence genes and candidate immunogens have been identified over the years, no effective vaccine is currently available to prevent R. equi disease in horses. Recently, bacterial vector vaccines have been shown to be promising for R. equi. In this study, the virulence-associated protein A (VapA) gene of R. equi was cloned into Protein Expression System small ubiquitin-related modifier (pET-SUMO) expression vectors and transferred into Escherichia coli BL21 (DE3). Also, adjuvant significantly affects the efficacy of recombinant vaccines. Therefore, native VapA and recombinant VapA were formulated with Immunostimuling Microparticle System (IMS 3012) or PetGel A (recommended for horses) and subcutaneously administered to mice. The immunization effect of four different vaccines was determined by assaying antibody titers and survival rates. The antibody response was slightly higher in the PetGel A formulations than IMS 3012. Survival rates were lower in the PetGel A formulations than IMS 3012. Given these results, recombinant VapA adjuvanted with PetGel A represents a promising formulation for developing new-generation R. equi vaccines.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"253-259"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases. N- 乙酰半胱氨酸在治疗犬血液原虫病引起的全血细胞减少症中可增强骨髓活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2033155.4328

Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das

{"title":"N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases.","authors":"Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das","doi":"10.30466/vrf.2024.2033155.4328","DOIUrl":"https://doi.org/10.30466/vrf.2024.2033155.4328","url":null,"abstract":"Canine hemoprotozoan diseases viz. ehrlichiosis and babesiosis are mostly associated with critical anemia and thrombocytopenia with pancytopenic changes, leading to multi-organ failure. For faster recovery of patients with complicated hemoprotozoan diseases, whole blood transfusion or bone marrow stimulating agents to produce more red blood cells (RBCs) and platelets might be helpful. Unfortunately, canine specific transfusion procedures are expensive and even not available in many developing countries. Development of alternate therapeutic modality by bone marrow stimulation to augment the production of RBCs and platelets and thus, to treat the critical pancytopenic patients is and an urgent necessity. N-acetylcysteine (NAC), acts as a precursor of reduced glutathione and increases the production of bone marrow B cells. It also improves viability and self-renewal capacity of stem cells and thus, boosts hematopoietic differentiation by protecting induced pluripotent stem cells. This study envisaged to develop alternate therapeutic approach to combat pancytopenia secondary to canine hemoprotozoan diseases. Bone marrow mediated aplastic pancytopenia was induced experimentally by administration of cyclophosphamide in rats. Bone marrow stimulating property of NAC was compared with desmopressin, another bone marrow stimulator, which revealed better in terms of hematobiochemical and histopathological changes. Results of rat model study were extrapolated in clinical canine hemoprotozoan cases having pancytopenia. Dogs treated with hemoprotozoan disease specific therapy along with NAC rendered favorable changes by haltering the progression of critical anemia and thrombocytopenia. Study revealed that supplementation of NAC along with canine hemoprotozoan specific therapy is beneficial to alleviate pancytopenia.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran. 伊朗养蜂场欧洲臭蝇病主、次病原检测的流行病学研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2026240.4232

Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani

{"title":"Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran.","authors":"Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani","doi":"10.30466/vrf.2024.2026240.4232","DOIUrl":"https://doi.org/10.30466/vrf.2024.2026240.4232","url":null,"abstract":"Infecting to Melissococcus plutonius, the primary cause of the European foulbrood (EFB) disease, can be followed by infecting to the secondary bacteria, such as Enterococcus faecalis and Brevibacillus laterosporus. The aim of this research was to diagnose EFB disease by tracking the causes of the disease in apiaries all over Iran. From 260 apiaries, honey bee samples were randomly collected. After samples preparation, the genomic DNA was extracted and specific primers were selected for interested bacteria. Using the conventional polymerase chain reaction (PCR) method for E. faecalis and B. laterosporus and nested-PCR method for M. plutonius, the target fragments were amplified. Desired standard bacteria and distilled water were used as positive and negative controls, respectively. Results showed that out of 260 samples from apiaries, 74 and three samples were positive for E. faecalis and B. laterosporus bacteria, respectively. Also, the results of nested-PCR showed that 58 samples were positive, of which only 12 samples were positive in the evaluation of E. faecalis. Results demonstrated that the highest and the least levels of the infection for M. plutonius and E. faecalis were in the south and east of the country, respectively. Results indicated that sometimes due to the excessive growth of secondary bacteria, the main bacteria can be removed from the environment. Also, findings proposed that those provinces with higher number of populations, followed by higher amount of air pollution, had more infected samples than others.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog. 两种严重的情况，一种突然丧失：狗的肠系膜扭转和免疫双丝虫炎。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023716.4197

Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović

{"title":"Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog.","authors":"Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović","doi":"10.30466/vrf.2024.2023716.4197","DOIUrl":"10.30466/vrf.2024.2023716.4197","url":null,"abstract":"Sudden death has been thoroughly well defined and investigated in human medicine and its definition is not as rigorously established in veterinary medicine. In this case a 7-year-old German Shepherd was brought to the Small Animals Clinic of the Faculty of Veterinary Medicine at the University of Belgrade in very poor state. Despite treatment, the dog died 15 min after being admitted to the clinic. Since poisoning was suspected, the owners requested an autopsy. The dog was taken to the Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Belgrade for necropsy. Pathomorphological examinations revealed rupture of the mesentery with mesenteric volvulus. In addition, a large number of adult forms of Dirofilaria immitis were found in the right atrium, ventricle and in truncus pulmonalis. Further examinations of the respiratory system revealed clusters with partially degraded adult parasites in the lobes. The dog died due to mesenteric volvulus which was a rare finding, with an even rarer finding that was lung nodules with adult D. immitis parasites.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"125-128"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram? 共同补充锌、锰和铜对公羊血浆睾酮、精子质量和抗氧化酶活性有影响吗？

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2025806.4222

Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic

{"title":"Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram?","authors":"Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic","doi":"10.30466/vrf.2024.2025806.4222","DOIUrl":"10.30466/vrf.2024.2025806.4222","url":null,"abstract":"Adding trace minerals to animal diets has a positive impact on reproductive performance. This study aimed to investigate whether supplementing zinc, manganese, and copper affects the testosterone level, sperm quality, and anti-oxidant enzyme activities in rams. Ten mature Afshari rams (2.50 years old; weighing 100 ± 3.29 kg) were fed a nutritionally adequate diet for 11 weeks, half of which receiving a sulfated zinc, manganese, and copper in their daily concentrates (supplemented group), while the other half served as a control group receiving no mineral in the concentrates. Ejaculate volume, as well as sperm concentration, motility, viability, morphology, and membrane integrity were assessed. From week five onwards, all parameters were significantly higher in the supplemented group. Additionally, the percentage of abnormal sperm was lower, anti-oxidant enzymes activities in the seminal plasma were improved, and plasma testosterone concentration was higher in the supplemented group compared to the control group. However, alkaline phosphatase activity was not significantly different. Furthermore, seminal plasma concentrations of copper, zinc, and manganese at the end of the study were higher in the supplemented group compared to pre-treatment levels, while these parameters were decreased in the control group. Overall, co-supplementing copper, zinc, and manganese in rams maintained the sperm quantity and quality, as well as seminal plasma anti-oxidant capacity, emphasizing the importance of studied elements in ram reproduction.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"167-174"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep. 正常格泽勒羊脉搏波多普勒超声心动图正常值。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2033315.4333

Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh

{"title":"Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep.","authors":"Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh","doi":"10.30466/vrf.2024.2033315.4333","DOIUrl":"10.30466/vrf.2024.2033315.4333","url":null,"abstract":"Echocardiography is a noninvasive, highly sensitive method for evaluating heart disease in veterinary medicine, crucial for measuring cardiac structures and functions. Despite its importance, limited data exist on sheep, particularly the Ghezel breed, a significant breed in Iran. This study aimed to establish normal echocardiographic values for Ghezel sheep, providing reference data for both clinical and research applications. We evaluated 15 healthy Ghezel sheep with a mean age of 16.50 ± 1.80 months and a mean weight of 42.69 ± 4.73 kg. Comprehensive clinical exams, biochemical and hematological tests and color Doppler echocardiography were performed. Descriptive results from standard right and left parasternal views in both longitudinal and transverse planes were obtained and compared to existing studies. The study successfully established pulse wave Doppler reference values for the Ghezel breed. These findings could be used in diagnosing heart disease in Ghezel sheep and would be beneficial in future research particularly in sheep used as an animal model for translation in human cardiac studies.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"229-234"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric, histometric and elemental profile of the metacarpal and metatarsal bones in adult Sanjabi sheep. 成年桑贾比羊掌骨和跖骨的形态计量学、组织计量学和元素特征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-05 DOI: 10.30466/vrf.2024.2034586.4349

Sajedeh Azizi, Nader Goodarzi, Saeed Ghaderi

{"title":"Morphometric, histometric and elemental profile of the metacarpal and metatarsal bones in adult Sanjabi sheep.","authors":"Sajedeh Azizi, Nader Goodarzi, Saeed Ghaderi","doi":"10.30466/vrf.2024.2034586.4349","DOIUrl":"10.30466/vrf.2024.2034586.4349","url":null,"abstract":"Bone structure has been widely studied in mammals, however, osteon structure in sheep has received relatively little attention, especially in terms of its location on the forelimbs and hindlimbs. The aim of this study was to investigate the histometric characteristics and mineral composition of the metacarpus and metatarsus of adult Sanjabi sheep. Metacarpal and metatarsal bones were collected from five adult Sanjabi sheep (n = 10). Morphometric measurements were performed on computed tomographic scan images. Histometric parameters were measured on histological sections. The mineral composition of the bone samples was detected using the X-ray fluorescence method. The diameter of the Haversian canal in the right metatarsus was significantly greater than that in the other bones. The smallest diameter of the Haversian canal was observed for the right metacarpus. The diameter and area of the osteons in the right metacarpal were significantly greater than those in the other bones. The amount of essential mineral elements was not significantly different among bones. Aluminum and lead were significantly greater in the left metatarsus. The highest amount of copper was observed in the left metacarpus. These results indicated that there was a greater load on the right limb. This compensatory mechanism might be used to put more weight on the right forelimb and reduce the pressure caused by the weight of the rumen on the left forelimb. However, to prove this hypothesis, more detailed and extensive studies are needed in the future.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"293-300"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran. 伊朗西阿塞拜疆牛和水牛溶血性曼海姆病分离株的基因分型和系统发育分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2027790.4254

Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh

{"title":"Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran.","authors":"Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh","doi":"10.30466/vrf.2024.2027790.4254","DOIUrl":"https://doi.org/10.30466/vrf.2024.2027790.4254","url":null,"abstract":"This study was conducted in West Azerbaijan province, Iran (37°27'18.022\" N, 45°0'0\" E) to investigate the genotyping and phylogenetic characterization of Mannheimia haemolytica in cattle and buffaloes from November 2022 to January 2024. Mannheimia haemolytica is a bacterium known to cause pasteurellosis pneumonia, a respiratory disease in ruminants, such as cattle and sheep. This is one of the main causes of economic losses in the feedlot industry. In addition to the deaths, treatment costs are also significant. The lung and nasal swab samples were collected from 378 cattle and buffaloes. The M. haemolytica was detected in 32 (8.46%) of the samples, with a notably higher isolation rate from lung tissue (56.25%; n = 18) compared to the nasal swabs (43.75%; n = 14). Interestingly, the study also revealed a seasonal pattern, with the highest isolation rates observed during January, February, and March. Multi-locus sequence typing demonstrated that all isolates belonged to sequence type 1 (ST1) within clonal complex 28. This finding is consistent with the global prevalence of ST1 in bovine isolates, indicating widespread distribution. Phylogenetic analysis revealed a strong correlation between ST1 and STs 30 and 54, highlighting the prevalence of ST1 in M. haemolytica among ruminants in West Azerbaijan, Iran. Further research is needed to investigate its potential for causing disease and its transmission pattern.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"11-18"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0