Veterinary Research Forum最新文献

{"title":"First report of histopathological and molecular characterizations of bovine herpesvirus-1 from outbreak at dairy farm in India.","authors":"Amit Kumar Verma, Vikas Jaiswal, Arbind Singh, Ramakant, Vishnu Kumar Rai","doi":"10.30466/vrf.2024.2028036.4260","DOIUrl":"10.30466/vrf.2024.2028036.4260","url":null,"abstract":"<p><p>Infectious bovine rhinotracheitis, caused by bovine herpesvirus-1 (BHV-1) is a highly contagious viral disease affecting bovines, and clinically characterized by pyrexia, inappetence, respiratory distress, dyspnoea, conjunctivitis, nasal discharge, and sometimes abortions. In the present study, buffalo dairy farm having high mortality was investigated. The buffaloes were suffering from high rectal temperature, conjunctivitis, severe respiratory distress, and nasal discharge. Tissue samples from upper respiratory tract were collected aseptically following post-mortem examination of died buffaloes. Tracheal tissue samples were then processed for histopathological examination and DNA isolation. The presence of BHV-1 in the tissue samples was confirmed by nested polymerase chain reaction using <i>glycoprotein B</i> gene primers. The present study reported for the first time the clinical signs, post-mortem lesions, histopathological evidence, and detection of DNA of BHV-1 <i>glycoprotein B</i> gene through nested polymerase chain reaction assay during an active outbreak in buffaloes in India. The findings of this study are crucial for improving the diagnosis of BHV-1 and ultimately reducing financial losses within dairy industry.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"301-304"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bioactive peptides on heat stress-induced testiculopathies in mature rats: immunohistopathological evidence.","authors":"Mahsa Valizadeh, Vahid Nejati, Ali Shalizar-Jalali, Ebrahim Najdegerami, Gholamreza Najafi","doi":"10.30466/vrf.2024.2032033.4318","DOIUrl":"10.30466/vrf.2024.2032033.4318","url":null,"abstract":"<p><p>Exposure to extreme temperature conditions such as occurs in certain occupations is known to induce male infertility. In humans and most of the mammals, it has been shown that whole-body heat stress (HS) decreases fertility and produces defective embryos. Hence, the present study aimed at gaining some insights into the mechanisms producing defects after HS. In the present study, 56 mature male Wistar rats were randomly categorized into eight groups (n = 7), including group 1: Control, groups 2: Bioactive peptides (BPs; 10.00 mg kg^-1), groups 3, 4, and 5: Heat-stressed (37.00, 39.00, and 43.00 ˚C for 20 min, respectively), and groups 6, 7, and 8: Heat-stressed along with BPs (10.00 mg kg^-1), respectively. All treatments were administered orally once <i>per</i> day. The HS was induced through the immersion of rat scrotums in a water bath. After 45 days, rats were sacrificed and left testes were removed, fixed, and used for histological and immunohistochemical studies. Harvested right testes were also used for oxidative stress assessments and molecular analyses. Heat stress increased testicular tissue damage, elevated oxidative stress and reactive oxygen species production, and increased germ cells apoptosis, <i>p53</i> and <i>caspase 3</i> expressions, and <i>Bax/Bcl-2</i> ratio. Treatment with BPs as a substance with anti-oxidant properties ameliorated the damage caused by HS. The results of this study highlight the protective role of BPs in the reproductive tract under HS. Bioactive peptides may have potential function against testicular tissue oxidative stress and apoptosis.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"106-116"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective effect of royal jelly on dibutyl phthalate-induced liver injury in rats.","authors":"Mahdieh Nezami Majd, Goudarz Sadeghi-Hashjin, Hassan Malekinejad, Ali Rassouli","doi":"10.30466/vrf.2024.2033878.4338","DOIUrl":"10.30466/vrf.2024.2033878.4338","url":null,"abstract":"<p><p>Phthalate esters, such as dibutyl phthalate (DBP), are extensively utilized and human and animal exposure leads to serious toxic effects, including hepatotoxicity. In the present study the protective effects of royal jelly (RJ) on DBP-induced liver damage was investigated. A total number of 40 Wistar albino rats were randomly divided into eight groups (n = 5): control (corn oil), DBP (500 mg kg^-1), RJ (200 mg kg¹), Quercetin (QCN; 50.00 mg kg^-1), RJ (100 mg kg^-1) + DBP, RJ (200 mg kg^-1) + DBP, RJ (300 mg kg^-1) + DBP, QCN (50.00 mg kg^-1) + DBP. After 28 days of daily oral gavage treatment, animals were euthanized. The insulin resistance index, lipid profile and hepatic enzymes were measured on the collected serum samples. Moreover, oxidative and nitrosative stress biomarkers were determined in the liver. Histopathological alterations and ultimately cytochrome P450 2E1 (CYP2E1) activity was also assessed. Data obtained revealed that RJ significantly reduced the insulin resistance index and liver enzymes level in RJ-DBP groups. At the same time, RJ recovered the DBP-induced oxidative stress and restored the DBP-depleted glutathione. Moreover, RJ improved lipid profile and reduced significantly the DBP-induced hepatic CYP 2E1 activity in RJ-DBP groups. Dibutyl phthalate induced-hepatic damage such as necrosis of hepatocytes and scattered bleeding was alleviated in RJ-DBP group. Our data suggested that the administration of RJ could protect the DBP-induced hepatic functional and structural alterations. The RJ protective effects might be attributed to its antioxidant and anti-inflammatory properties and reduced CYP 2E1 activity.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"97-105"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture.","authors":"Dragan Jevrem Ristanović, Jelena Vladimir Francuski Andrić, Milena Danilo Radaković, Kristina Adam Spariosu, Ivan Željko Vlahek, Marija Tomo Lipar, Petar Sreten Milosavljević, Mario Ivan Kreszinger","doi":"10.30466/vrf.2023.2017099.4089","DOIUrl":"10.30466/vrf.2023.2017099.4089","url":null,"abstract":"<p><p>Cranial cruciate ligament rupture is one of the most common causes of osteoarthritis in dogs. Surgical stabilization is obligatory and tibial plateau levelling osteotomy (TPLO) is the most commonly used surgical technique. Studies on the long-term monitoring of matrix metallo-proteinases (MMPs) and acute-phase proteins are limited, especially those with parallel monitoring of changes in the serum, synovial fluid (SF) and urine. We aimed to describe long-term changes in 1) MMPs: gelatinases and caseinases, 2) APPs: ceruloplasmin, haptoglobin and paraoxonase-1, and 3) the correlation of MMPs in the serum, SF and urine with lameness in dogs 2 and 6 months undergoing TPLO. From 17 dogs diagnosed with cranial cruciate ligament rupture, sera, SF and urine samples were collected preoperative 2 and 6 months after the surgery. Relative activity (RA) of MMPs was measured in all samples using zymography. Acute-phase proteins were measured in the serum and SF using spectrophotometry and agarose gel electrophoresis. Relative activity of MMPs in serum was not change at different sampling points. In SF, a marked decrease in MMPs RA was evident, however, only RA of caseinases was significantly reduced during the recovery period. In urine, RA of caseinases was positively correlated with the lameness score with decreasing activity trend during time. Serum and synovial acute-phase proteins were not changed after surgery, which was consistent with the agarose gel analyses. Decreased activity of caseinases pointed to reduced degradation of extracellular matrix after TPLO. Synovial and urine caseinases are potential biomarkers in predicting the recovery outcome following stifle stabilization.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"63-69"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain.","authors":"Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi","doi":"10.30466/vrf.2024.2032492.4322","DOIUrl":"10.30466/vrf.2024.2032492.4322","url":null,"abstract":"<p><p>Infectious bronchitis, being caused by a coronavirus, is a significant disease affecting broiler and layer chickens, leading to substantial losses in the poultry industry due to the high mortality rates and decreased egg yield. Nearly 30 serotypes and 100 variants were described to date; developed vaccines are being for some severe cases, like the Massachusetts strain, to mitigate the effects. Determining the vaccinal strain's titer is crucial for creating an effective vaccine, and calculating the virus infectivity in the egg embryo is very important using dilutions ranging from 10^-3 to 10^-8, from each dilution 0.10 mL is used. The aim of this study was to determine the effects of the avian bronchitis virus injected into the allantoic cavity of ten days old embryonated eggs. Real-time polymerase chain reaction tests determined the viral load in the allantoic fluid. The embryos were removed to study gross injuries. The trachea, lung, and mesonephros were removed and submitted for histopathological studies, and nuclear factor-kappa B immunofluorescence analysis. The results revealed that the dilution of one-thousandth of the virus in the embryos caused the highest organ damage and viral replication. Varying degrees of hyperemia, edema, cellular infiltration, and degeneration were observed in the trachea, lung, and mesonephros depending on the virus dilution. This study provides valuable insights into the pathogenesis of the avian bronchitis virus, and has a potential impact on achieving an effective vaccine.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"161-166"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating serological evidence of Schmallenberg virus in cattle in eastern Algeria.","authors":"Nadia Djellata, Abdelkrim Yahimi, Christian Hanzen","doi":"10.30466/vrf.2024.2026853.4238","DOIUrl":"10.30466/vrf.2024.2026853.4238","url":null,"abstract":"<p><p>Schmallenberg virus (SBV) is a newly identified virus belonging to the Orthobunyavirus genus, of the Bunyaviridae family, and transmitted by haematophagous arthropods in particular mosquitoes and biting midges of the Culicoides genus. The SBV is known to cause reproductive disorders in ruminants mainly abortions, stillbirths and congenital malformations (hydranencephaly and arthrogryposis syndromes). The aim of this study was to investigate the presence of SBV in dairy cattle in Algeria. Between September 2023 and December 2023, blood serum samples from 300 dairy cows from 75 dairy farms in north eastern Algeria were tested for SBV antibodies using a commercial indirect enzyme-linked immunosorbent assay kit. Individual seroprevalence was 38.33% (115/300; 95.00% confidence interval: 32.83 - 43.83), while herd seroprevalence was 41.33% (31/75; 95.00% confidence interval: 30.18 - 52.47). In addition, the results of this study revealed that SBV seroprevalence at individual or herd level was high and not negligible confirming the presence of SBV in the regions studied in Algeria. In conclusion, more in-depth studies are recommended concerning the molecular proof, origin and pathogenesis of SBV in ruminants mainly those linked to reproductive disorders as well as the study of the various associated risk factors.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"129-132"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of bovine group A rotavirus, bovine coronavirus, <i>Escherichia</i> <i>coli</i> K99, <i>Cryptosporidium</i> spp., <i>Giardia</i> spp. in calf diarrhea with one-step duplex RT-PCR: histopathological and immunochromotographic methods.","authors":"Bunyamin Irehan, Mustafa Ozkaraca, Irem Gulactı, Levent Sener, Muhammed Fatih Dursun","doi":"10.30466/vrf.2024.2025299.4208","DOIUrl":"10.30466/vrf.2024.2025299.4208","url":null,"abstract":"<p><p>Infections causing diarrhea in cattle breeding pose a substantial threat to calf breeding and health, causing considerable economic losses worldwide, including in Türkiye. The primary causative agents of calf diarrhea include bovine group A rotavirus, bovine coronavirus (BCoV), <i>Escherichia coli</i> K99, <i>Cryptosporidium</i> spp., and <i>Giardia</i> spp. The objective of this study was to diagnose the pathogens present in samples taken from calves exhibiting diarrhea symptoms in the Elaziğ province of Türkiye. The study material consisted of 85 fecal samples from calves up to 30 days of age with diarrhea symptoms and 42 small intestine samples from calves died as a result of diarrhea symptoms after necropsy. Following total RNA isolation from the samples, one-step duplex reverse transcription polymerase chain reaction (RT-PCR) analysis was conducted to identify the bovine rotavirus (BRV) and BCoV. Our results indicated that 24 of the samples were positive for BRV, 13 were positive for BCoV, and 25 were positive for BRV and BCoV in the form of mixed infection. The immunochromatographic analysis revealed that three samples were positive for <i>E. coli</i> K99 antigens, 12 for <i>Cryptosporidium</i> spp. antigens, and 28 for <i>Giardia</i> spp. antigens. Our results indicate that one-step duplex RT-PCR, immunohistochemistry, and immunochromatography methods can be valuable tests to be used in routine diagnostic laboratories for the detection of pathogens associated with calf diarrhea.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"221-227"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological development of the ovary in the <i>Alectoris chukar</i> at embryonic and pre-pubertal stages.","authors":"Morvarid Teymouri, Masoumeh Kheirabadi, Abolghasem Nabipour","doi":"10.30466/vrf.2024.2035219.4357","DOIUrl":"10.30466/vrf.2024.2035219.4357","url":null,"abstract":"<p><p><i>Alectoris chukar</i> (AC) is a common model organism in biological research. To understand oogenesis and folliculogenesis mechanisms in bird reproduction, we analyzed the ovarian tissue structure of AC at embryonic and pre-pubertal stages. Fertilized eggs, newborn chicks and juvenile AC were used to study the tissue structure of female gonads. Sections of ovaries were prepared and examined using various histological techniques including Hematoxylin and Eosin, Periodic acid-Schiff and Masson's trichrome. Semi-thin and ultra-thin sections of ovary in newly-hatched chicks were prepared for study by electron microscope. The study revealed asymmetry between the left and right ovaries, with a larger left ovary. The functional left ovary exhibited a cortex and medulla, containing somatic and germ cells, with an increase in germ cell number, size and volume leading to cortex thickening. Meiosis division of germ cells and oocyte formation were observed with pre-follicular cells surrounding them. Electron microscopy revealed mitochondria and desmosome cell junctions in germ cells. Our study provided insights into tissue changes in ovaries and germ cells at different developmental stages of AC embryos, newly-hatched chicks and juvenile AC. The results suggested that cortex thickening and germ cell mitochondria density could be used as hallmarks of healthy AC maturity under normal physiological conditions. Further research should explore the impact of growth factors, hormones and environmental factors to unravel avian ovarian development complexities and improve AC reproductive biology knowledge.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"39-344"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats.","authors":"Kianoush Mohammadnehjad, Rahim Mohammadi, Ali Soleimanzadeh, Ali Shalizar-Jalali, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2025.2046637.4559","DOIUrl":"10.30466/vrf.2025.2046637.4559","url":null,"abstract":"<p><p>Buildup of reactive oxygen species during testicular torsion causes oxidative stress and ischemia-reperfusion (I/R) injury in testis. The purpose of this study was to investigate influence of β-cryptoxanthin (BCX) on I/R injury in testicular torsion/detorsion in mature rats. Thirty mature male Wistar rats were divided into five groups of six animals each, including sham group: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hr with a 720-degree rotation, I/R group: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hr with a 720-degree rotation, I/R/Oil group: In this group, a midline scrotum cut was performed, the testicles were taken out, ischemia was created for 2 hr with a 720-degree rotation, and at the end of ischemia 100 µL of corn oil (BCX solvent) was injected intraperitoneally, I/R/BCX10 group: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (10.00 µg kg^-1) at the end of ischemia, and I/R/BCX40: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (40.00 µg kg^-1) at the end of ischemia. Evaluations were based on histopathological and spermatological parameters and oxidative stress assessments. Histopathological spermatological and oxidative stress parameters values obtained from I/R/BCX40 were significantly different from those of other groups (<i>p</i> < 0.05). It could be concluded that BCX could ameliorate testicular injuries in acute testicular torsion/detorsion in mature rats.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"277-284"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-microbial resistance to β-lactams and prevalence of colicin genes among phylotypes of <i>Escherichia coli</i> isolates from hedgehogs.","authors":"Maziar Jajarmi, Shademan Sahraei, Parvin Mohseni, Pouneh Hajipour, Pouya Reshadi, Fatemeh Heydari, Baharak Akhtardanesh, Niousha Ghanbarpour, Majid Rashidi, Nasrin Adib, Zeinab Abiri, Haniyeh Amirinezhad","doi":"10.30466/vrf.2024.2029461.4284","DOIUrl":"10.30466/vrf.2024.2029461.4284","url":null,"abstract":"<p><p>Several bacteria, such as <i>Escherichia</i> <i>coli</i>, <i>Pseudomonas</i>, and <i>Staphylococcus</i> are considered as indicators of anti-microbial resistance (AMR) in a wide range of hosts and environments, because they may transfer AMR genes to important pathogenic bacteria. Hedgehog is one of the most important wild species living in urban areas. So, this study aimed to determine AMR against β-lactams and prevalence of colicin genes among various phylotypes of <i>E. coli</i> isolates from hedgehogs in the Kerman, Iran. Totally, 105 <i>E. coli</i> isolates were obtained from 21 hedgehogs (five isolates from each hedgehog). Resistances to the ampicillin (79.10%), cefotaxime (66.70%), and amoxicillin-clavulanate (62.00%) were the most prevalent, and resistance against ceftiofur (39.10%), ceftazidime (39.10%), and ceftriaxone (34.30%) had the lowest prevalence rates. In phenotypic tests, 2.90% of the isolates were extended spectrum β-lactamase producers. The prevalence of β-lactam resistance genes was 26.60% for <i>bla</i> _TEM, 3.80% for <i>bla</i> _CTX _- _M, 8.50% for <i>bla</i> _SHV, and 1.90% for <i>bla</i> _CMY. The frequency of colicin genes, including <i>E1</i>, <i>V</i>, <i>E2</i>-<i>E9</i>, and <i>Ia</i>.<i>Ib</i> was 5.71, 4.76, 10.47, and 11.42%, respectively. All <i>E. coli</i> isolates were negative for <i>5.10.K</i>, <i>Y.U</i>, and <i>A.N.S4</i> genes. Phylogenetically, B1 (49.50%), A (40.90%), and D (5.70%) were identified among the isolates, and 3.80% remained unknown. Wildlife could be considered as a bio-marker to determine the environmental dissemination of AMR. Also, hedgehog may be an important reservoir of antibiotic-resistant and non-pathogenic <i>E. coli</i> strains in urban environments. This study highlights the necessity of <i>E. coli</i> surveillance among domestic and wild animals.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"407-414"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}