Veterinary Research ForumPub Date : 2025-01-01Epub Date: 2025-03-15DOI: 10.30466/vrf.2024.2025806.4222
Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic
{"title":"Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram?","authors":"Sona Zargari, Armin Towhidi, Kamran Rezayazdi, John Patrick Kastelic","doi":"10.30466/vrf.2024.2025806.4222","DOIUrl":"10.30466/vrf.2024.2025806.4222","url":null,"abstract":"<p><p>Adding trace minerals to animal diets has a positive impact on reproductive performance. This study aimed to investigate whether supplementing zinc, manganese, and copper affects the testosterone level, sperm quality, and anti-oxidant enzyme activities in rams. Ten mature Afshari rams (2.50 years old; weighing 100 ± 3.29 kg) were fed a nutritionally adequate diet for 11 weeks, half of which receiving a sulfated zinc, manganese, and copper in their daily concentrates (supplemented group), while the other half served as a control group receiving no mineral in the concentrates. Ejaculate volume, as well as sperm concentration, motility, viability, morphology, and membrane integrity were assessed. From week five onwards, all parameters were significantly higher in the supplemented group. Additionally, the percentage of abnormal sperm was lower, anti-oxidant enzymes activities in the seminal plasma were improved, and plasma testosterone concentration was higher in the supplemented group compared to the control group. However, alkaline phosphatase activity was not significantly different. Furthermore, seminal plasma concentrations of copper, zinc, and manganese at the end of the study were higher in the supplemented group compared to pre-treatment levels, while these parameters were decreased in the control group. Overall, co-supplementing copper, zinc, and manganese in rams maintained the sperm quantity and quality, as well as seminal plasma anti-oxidant capacity, emphasizing the importance of studied elements in ram reproduction.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"167-174"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2025-01-01Epub Date: 2025-04-15DOI: 10.30466/vrf.2024.2033315.4333
Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh
{"title":"Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep.","authors":"Mohammad Honarjoo, Seyed Mohammad Hashemi-Asl, Bahram Dalir-Naghadeh, Alireza Rostamzadeh","doi":"10.30466/vrf.2024.2033315.4333","DOIUrl":"10.30466/vrf.2024.2033315.4333","url":null,"abstract":"<p><p>Echocardiography is a noninvasive, highly sensitive method for evaluating heart disease in veterinary medicine, crucial for measuring cardiac structures and functions. Despite its importance, limited data exist on sheep, particularly the Ghezel breed, a significant breed in Iran. This study aimed to establish normal echocardiographic values for Ghezel sheep, providing reference data for both clinical and research applications. We evaluated 15 healthy Ghezel sheep with a mean age of 16.50 ± 1.80 months and a mean weight of 42.69 ± 4.73 kg. Comprehensive clinical exams, biochemical and hematological tests and color Doppler echocardiography were performed. Descriptive results from standard right and left parasternal views in both longitudinal and transverse planes were obtained and compared to existing studies. The study successfully established pulse wave Doppler reference values for the Ghezel breed. These findings could be used in diagnosing heart disease in Ghezel sheep and would be beneficial in future research particularly in sheep used as an animal model for translation in human cardiac studies.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"229-234"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2025-01-01Epub Date: 2025-01-15DOI: 10.30466/vrf.2024.2027790.4254
Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh
{"title":"Genotyping and phylogenetic analysis of <i>Mannheimia haemolytica</i> isolates from cattle and buffaloes of West Azerbaijan, Iran.","authors":"Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh","doi":"10.30466/vrf.2024.2027790.4254","DOIUrl":"https://doi.org/10.30466/vrf.2024.2027790.4254","url":null,"abstract":"<p><p>This study was conducted in West Azerbaijan province, Iran (37°27'18.022\" N, 45°0'0\" E) to investigate the genotyping and phylogenetic characterization of <i>Mannheimia haemolytica</i> in cattle and buffaloes from November 2022 to January 2024. <i>Mannheimia haemolytica</i> is a bacterium known to cause pasteurellosis pneumonia, a respiratory disease in ruminants, such as cattle and sheep. This is one of the main causes of economic losses in the feedlot industry. In addition to the deaths, treatment costs are also significant. The lung and nasal swab samples were collected from 378 cattle and buffaloes. The <i>M. haemolytica</i> was detected in 32 (8.46%) of the samples, with a notably higher isolation rate from lung tissue (56.25%; n = 18) compared to the nasal swabs (43.75%; n = 14). Interestingly, the study also revealed a seasonal pattern, with the highest isolation rates observed during January, February, and March. Multi-locus sequence typing demonstrated that all isolates belonged to sequence type 1 (ST1) within clonal complex 28. This finding is consistent with the global prevalence of ST1 in bovine isolates, indicating widespread distribution. Phylogenetic analysis revealed a strong correlation between ST1 and STs 30 and 54, highlighting the prevalence of ST1 in <i>M. haemolytica</i> among ruminants in West Azerbaijan, Iran. Further research is needed to investigate its potential for causing disease and its transmission pattern.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"11-18"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on syndemic infection of porcine reproductive and respiratory syndrome virus with porcine circovirus 2 in backyard pigs of Mizoram, India.","authors":"Amitava Paul, Tridib Kumar Rajkhowa, Kiran Jayappa","doi":"10.30466/vrf.2024.2023688.4187","DOIUrl":"10.30466/vrf.2024.2023688.4187","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus 2 (PCV2) are among one of the most prevalent and important infectious agents, which cause a severe economic impact on pig farming worldwide. Circulation of these viruses together in the same pig population may lead to the syndemic infection with altered pathogenicity and thereby pathology and clinical manifestation of the diseases. The present study evaluated syndemic infection between PRRSV and PCV2 in the pig population reared in the backyard farms in Mizoram state of India. The syndemicity between PRRSV and PCV2 was confirmed by clinico-pathological studies followed by simultaneous detection of both the viruses in tissue samples by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR), immunohisto-chemistry and SYBR green-based real-time PCR / RT- PCR. The syndemicity resulted to a more severe respiratory disease in affected pigs. Pathological studies on affected pigs revealed a combined picture of hemorrhagic lesions with lymphadenopathy. The SYBR green-based absolute quantification assay estimated the PCV2 load in more quantities than that of PRRSV in all the tissues. The highest PRRSV load was detected in the lungs, while the highest PCV2 load was detected in mesenteric and inguinal lymph nodes. The co-circulation of different pathogenic viruses and their persistent infections in a population is always a matter of great concern to pig producers across the world. The present findings highlighted the similar situation with syndemic infection of PRRSV with PCV2 for the first time in Mizoram, India, that caused severe respiratory disease in affected pigs.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"81-88"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An outbreak of Fusobacterium necrophorum associated foot rot in a herd of four-horned antelope (<i>Tetracerus quadricornis</i>) in India.","authors":"Susitha Rajkumar, Loveleen Maria Vaz, Mathavan Anandhi, Karikalan Mathesh, Shivasharanappa Nayakvadi, Solomon Rajkumar, Shirish Dadarao Narnaware","doi":"10.30466/vrf.2024.2028598.4269","DOIUrl":"https://doi.org/10.30466/vrf.2024.2028598.4269","url":null,"abstract":"<p><p>An outbreak of contagious foot rot due to <i>Fusobacterium necrophorum</i> infection was reported in a herd of four-horned antelopes (<i>Tetracerus quadricornis</i>) in a wildlife sanctuary at Goa, India. The infected animals showed signs of anorexia, limping and foot lesions, like erosions and sloughing of hooves. Mortality was recorded in two antelopes in which gross lesions, like generalized congestion of skeletal muscles and internal organs, and multi-focal to coalescing abscesses in lung, pleura, and diaphragm were observed. Histopathological evaluation of interdigital skin showed zones of coagulative necrosis, infiltration of mononuclear inflammatory cells, congestion, hemorrhage, and thrombosis in the dermis. Lung showed extensive areas of consolidation with alveolar edema, hyperemic alveolar capillaries, hemorrhage, and infiltration of inflammatory cells. The bacterial culture from heart blood, lung, liver, and kidney tissues revealed isolation of <i>F. necrophorum</i> being also confirmed by polymerase chain reaction (PCR) using amplification of <i>lktA</i> gene of <i>F. necrophorum</i>. In addition, the PCR also confirmed the presence of <i>F. necrophorum</i> DNA in the interdigital skin, lung, and liver tissues. Phylogenetic analysis showed that the <i>F. necrophorum</i> isolate from the present study grouped closely with isolates from Australia, China, Pakistan, and Iraq, as well as an Indian isolate from goat. The affected live animals were treated with oral antibiotics, anti-inflammatory medicines, and vitamin supplements for seven days, and recovered. This study confirmed the first outbreak of contagious foot rot and systemic infection with <i>F. necrophorum</i> in a herd of four-horned antelopes in India.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"51-56"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proteomic profiling of pseudorabies virus-infected PK-15 cells based on 4D label free analysis.","authors":"Shuaiwei Wang, Xiaoyong Chen, Houqiang Luo, Meng Wang, Junyu Liu, Qingsong Han","doi":"10.30466/vrf.2024.2026963.4239","DOIUrl":"10.30466/vrf.2024.2026963.4239","url":null,"abstract":"<p><p>Pseudorabies virus (PRV) heavily depends on host machinery to support its life cycle. Investigating the interaction between PRV and host could aid in the understandings of viral pathogenesis. In this study, we performed a 4D label free proteomic method to examine the differentially expressed proteins in porcine kidney PK-15 cells with PRV infection. The results showed that the levels of 661 proteins were significantly elevated and 693 proteins were markedly reduced. Furthermore, these altered proteins were primarily enriched in spliceosome, protein processing in endoplasmic reticulum (ER), RNA transport, and protein export. To ensure the reliability of the proteomic results, the protein levels of formin binding protein 11 and wolfram syndrome 1as components of spliceosome and ER were verified via western blotting and the results were consistent. Together, our data shed light on a new protein profiling induced by PRV infection and highlighted the importance of spliceosome and ER in PRV replication which could promote understandings of host-PRV interplay.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"141-147"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of using a Rovabio<sup>®</sup> Plus multi-enzyme on production and incubation performance, blood parameters and duodenum morphology of broiler breeders at age of 45 - 60 weeks.","authors":"Payam Panahi Moghaddam, Yahya Ebrahimnezhad, Naser Maheri-Sis, Habib Aghdam Shahryar, Abolfazl Aghajanzadeh-Golshani, Abolfazl Gorbani","doi":"10.30466/vrf.2024.2033379.433","DOIUrl":"10.30466/vrf.2024.2033379.433","url":null,"abstract":"<p><p>The aim of this study was to investigate the effects of using a Rovabio<sup>®</sup> Plus multi-enzyme on production and reproductive performance, duodenum morphology and biochemical parameters in 45 - 60 weeks old broiler breeders. For this purpose, 260 broiler breeders of Ross 308 strain were used in a completely randomized design with four treatments and five replications (13 hens and one rooster in each replication). Experimental treatments included: 1) Positive control diet (diet with standard energy and phosphorus and without multi-enzyme), 2) Negative control diet (diet in terms of energy 5.00% and phosphorus 50.00% less than the positive control diet, 3) Negative control diet with 1.00 g kg<sup>-1</sup> Rovabio<sup>®</sup> Plus multi-enzyme and 4) Positive control diet with 1.00 g kg<sup>-1</sup> Rovabio<sup>®</sup> Plus multi-enzyme. The results showed that negative control diet caused a significant decrease in the egg production percentage, egg weight, egg mass, settable eggs, fertile eggs, hatchability and one grade chickens, increased the feed conversion ratio and embryonic losses. Addition of 1.00 g kg<sup>-1</sup> of multi-enzyme to negative control diet made the above parameters similar to the positive control treatment, however, the positive control treatment supplemented with multi-enzyme had a better performance compared to other treatments. This research showed that dietary supplementation of Rovabio<sup>®</sup> Plus multi-enzyme in broiler breeders at the age of 45 - 60 weeks led to the improvement of productive performance and incubation in negative control diet.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"245-252"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2025-01-01Epub Date: 2025-03-15DOI: 10.30466/vrf.2024.2027690.4249
Muhammet Bahaeddin Dörtbudak, Uğur Şeker, Muhammet Demircioğlu, Ismail Demircioğlu
{"title":"Effect of <i>Gundelia tournefortii</i> extract on diabetic gastropathy: involvement of inflammation, apoptosis, oxidative stress, and histopathology.","authors":"Muhammet Bahaeddin Dörtbudak, Uğur Şeker, Muhammet Demircioğlu, Ismail Demircioğlu","doi":"10.30466/vrf.2024.2027690.4249","DOIUrl":"10.30466/vrf.2024.2027690.4249","url":null,"abstract":"<p><p>In this study, the effect of <i>Gundelia tournefortii</i> (GT) extract against diabetic gastropathy was investigated by pathological methods. The animal groups were designed as the control, diabetes, diabetes + GT50, diabetes + GT100, and diabetes + GT200 groups. No treatment was applied to the control group. The other groups received 45.00 mg kg<sup>-1</sup> streptozotocin intraperitoneally on the experimental day. The treatment groups were also given 50.00, 100, and 200 mg kg<sup>-1</sup> of GT extract daily by gavage for 21 days. Tissues were stained with Hematoxylin and Eosin for histopathological examination. Immunohistochemical staining was performed to reveal the presence of inflammation (tumor necrosis factor alpha), apoptosis (cysteine aspartate specific proteases-3), and oxidative stress (heat shock protein-27). Histopathological examination revealed no pathological lesion in the control group. In the diabetes group, mucosal tissue damage, and vascular and inflammatory changes were observed. In the treatment groups, GT decreased histopathological findings in parallel with the dose increase. Immunohistochemical examination revealed no immunopositivity in the control group, while severe immunopositivity was observed in the diabetes groups in terms of inflammation, apoptosis, and oxidative stress. In the treatment groups, there was a decrease in the severity of immunopositivity's depending on the dose increase. As a result of this study, which has not been done before, GT was found to have a protective effect against gastropathy, being an important complication of diabetes, and this study is thus an important reference point for future research and promises new hope for the patients.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"133-139"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of the effect of <i>Tarantula cubensis</i> alcoholic extract on cadmium embryotoxicity.","authors":"Rahmi Canbar, Gokhan Akcakavak, Muhittin Uslu, Mustafa Sedat Arslan, Harun Kizilay","doi":"10.30466/vrf.2024.2031444.4313","DOIUrl":"10.30466/vrf.2024.2031444.4313","url":null,"abstract":"<p><p><i>Tarantula cubensis</i> alcoholic extract (TCAE) is a homeopathic product used in the veterinary field. This study aimed to determine the effects of TCAE on cadmium (Cd) toxicity in the embryo. The study used 220 fertile, incubated chicken eggs divided into 11 equal groups on the 7<sup>th</sup> day of incubation. The groups comprised untreated and physiological saline control groups, a group with TCAE alone, four groups with varying doses of Cd alone and four groups with the same doses of Cd plus TCAE. At the end of the incubation period, the eggs were opened, kidney and liver tissue samples were taken for histopathology and the number of dead and living embryos were recorded. In the prsent study, the median lethal dose of Cd was determined to be 0.029 mg <i>per</i> egg and the median lethal dose of Cd plus TCAE was determined to be 0.020 mg <i>per</i> egg. The histopathological examinations determined that kidney and liver damage were increased when TCAE and Cd were administered together, that was higher than when Cd was given alone. Thus, TCAE, which had no toxic effect on the embryo when used alone, might increase the embryotoxic activity of Cd. However, more detailed studies are needed.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"203-208"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of cage density and meat storage period on some meat quality parameters in brown and white spent hens.","authors":"Uğur Özentürk, Ayşe Uysal, Ekrem Laçin, Soner Uysal, Sevda Urçar Gelen, Hayrunnisa Özlü","doi":"10.30466/vrf.2024.2025425.4210","DOIUrl":"10.30466/vrf.2024.2025425.4210","url":null,"abstract":"<p><p>This study was conducted to determine the effect of different cage densities and meat storage periods on some meat quality parameters in drumstick and breast meat of brown and white spent hens. The study used three different cage densities as low (5 hens <i>per</i> cage), normal (seven hens <i>per</i> cage) and high (10 hens <i>per</i> cage) including 396 hens as 198 Hy-Line Brown (HB) and 198 Isa Tinted (IT). The feeding of chickens was <i>ad libitum</i> (20 - 60 weeks). At the end of the study, a total number of 54 chickens (27 HB and 27 IT) were slaughtered. Some chemical and microbiological analyses were carried out by separating the drumstick and breast area of the slaughtered chickens. According to the results of the research, the redness and yellowness values of the breast area were higher in the ITs. The effect of cage density was significant only for yellowness in the drumstick area. The effects of storage time, lightness and yellowness in the drumstick area and lightness value in the breast area were found to be significant. Bacterial density and thiobarbituric acid reactive substances values of both breast and drumstick regions of spent hens were higher in HBs, while pH values were higher in ITs. The bacterial density in the meat was increased during the storage period. In conclusion, cage density, genotype and storage time affected the microbiological and chemical quality of spent chicken meat. With this result, it can be said that meat quality studies, which mostly focus on broilers, are also important in spent hens.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"71-79"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}