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Effect of nanocurcumin on fertility in murine model of polycystic ovary syndrome. 纳米姜黄素对多囊卵巢综合征小鼠模型生育力的影响
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2006604.3935
Zahra Aaly-Gharibeh, Mohammadreza Hosseinchi, Ali Shalizar-Jalali
{"title":"Effect of nanocurcumin on fertility in murine model of polycystic ovary syndrome.","authors":"Zahra Aaly-Gharibeh, Mohammadreza Hosseinchi, Ali Shalizar-Jalali","doi":"10.30466/vrf.2023.2006604.3935","DOIUrl":"10.30466/vrf.2023.2006604.3935","url":null,"abstract":"<p><p>The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, <i>in vitro</i> fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. Fifty adult female mice were randomly categorized into five equal groups including non-treated control and PCOS (receiving 0.20 mg estradiol valerate (EV) intra-peritoneally once a day for 21 days) as well as NCC<sub>12.50</sub> + PCOS, NCC<sub>25</sub> + PCOS and NCC<sub>50</sub> + PCOS (receiving respectively 12.50, 25.00 and 50.00 mg kg<sup>-1</sup> NCC daily along with EV injection through oral gavages for 21 days) groups. Subsequently, ovarian histo-architecture and total anti-oxidant capacity, and malonaldehyde and catalase levels as well as <i>in vitro</i> fertilizing potential, early embryonic development and serum testosterone concentration were analyzed. Results showed that NCC in a dose-dependent manner improved ovarian cyto-architectural organization and oxidant/anti-oxidant balance along with IVF rate and pre-implantation embryo development in PCOS mice. These findings revealed that NCC at the doses of 25.00 and 50.00 mg kg<sup>-1</sup> could alleviate PCOS-linked reproductive disruptions in female mice.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"113-117"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First report of Hepatozoon felis infection in a domestic cat (Felis catus) in Iran. 伊朗首次报告家猫(Felis catus)感染猫肝吸虫。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2023.2005996.3928
Bijan Esmaeilnejad, Awat Samiei, Zahra Darabizade, Mohsen Akhoondi, Romina Salavati, Bahar Ahooghalandari, Vahid Fathipour, Arash Akhavan, Armin Fakheri
{"title":"First report of <i>Hepatozoon felis</i> infection in a domestic cat (<i>Felis catus</i>) in Iran.","authors":"Bijan Esmaeilnejad, Awat Samiei, Zahra Darabizade, Mohsen Akhoondi, Romina Salavati, Bahar Ahooghalandari, Vahid Fathipour, Arash Akhavan, Armin Fakheri","doi":"10.30466/vrf.2023.2005996.3928","DOIUrl":"10.30466/vrf.2023.2005996.3928","url":null,"abstract":"<p><p>A 6-year-old male domestic short-haired cat (<i>Felis catus</i>) was presented with weakness, anorexia, fever, icterus, a painful abdomen, ruffled hair and a tick infestation, and it had no prior surgery. Laboratory analysis revealed left-shifted neutrophils, thrombocytopenia, low albumin content and high serum bilirubin concentration as well as activities of hepatic enzymes including alanine aminotransferase and aspartate aminotransferase. Azotemia and increased serum levels of creatinine and urea were also recorded. In Giemsa-stained blood smear, <i>Hepatozoon</i> gamonts were observed within neutrophils. Species-specific polymerase chain reaction assay was used to amplify an approximately 590 bp fragment of <i>18S rRNA</i> gene and confirmed <i>Hepatozoon felis</i> infection. The cat was treated with imidocarb dipropionate and doxycycline and recovered completely. Six-month follow-up showed no recurrence. This study reveals the presence of <i>H. felis</i> in Iran and it should be considered in differential diagnosis in febrile and icteric cats. To the authors' knowledge, this is the first description of <i>H. felis</i> infection in a cat in Iran.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"211-214"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Key molecules regulating the blood meals of Rhipicephalus sanguineus (Acari: Ixodidae) revealed by transcriptomics. 转录组学揭示了调控红腹角雉(Acari: Ixodidae)血餐的关键分子。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2011271.4007
Yajun Lu, Yijia Xu, Chenghang Yu, Shi Cheng, Qianfeng Xia, Zheng Bin
{"title":"Key molecules regulating the blood meals of <i>Rhipicephalus sanguineus</i> (Acari: <i>Ixodidae</i>) revealed by transcriptomics.","authors":"Yajun Lu, Yijia Xu, Chenghang Yu, Shi Cheng, Qianfeng Xia, Zheng Bin","doi":"10.30466/vrf.2024.2011271.4007","DOIUrl":"10.30466/vrf.2024.2011271.4007","url":null,"abstract":"<p><p><i>Rhipicephalus sanguineus,</i> a repulsive obligate blood feeder, is a three-host tick inflicting tremendous damage. Blood-sucking initiates tick-pathogen-host interactions along with alterations in the expression levels of numerous bioactive ingredients. Key molecules regulating blood meals were identified using the transcriptomic approach. A total number of 744 transcripts showed statistically significantly differential expression including 309 significantly upregulated transcripts and 435 significantly downregulated transcripts in semiengorged female ticks compared to unfed ticks, all collected in 2021. The top 10 differentially upregulated transcripts with explicit functional annotations included turripeptide OL55-like protein, valine tRNA ligase-like protein and ice-structuring glycoprotein-like protein. The top 10 differentially down-regulated transcripts were uncharacterized proteins. Gene Ontology (GO) enrichment analysis revealed four associated terms in the cellular component category and 16 in the molecular function category among the top 20 terms. Differentially expressed genes (DEGs) were enriched in GO terms ID 0000323 (lytic vacuole) and ID 0005773 (vacuole). The top 20 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included metabolism, cellular processes, organismal systems and human diseases. The DEGs were enriched in the KEGG term ID: ko-04142 (lysosome pathway) associated with intracellular digestion in the tick midgut epithelium. Molecular markers annotated via comparative transcriptomic profiling were expected to be candidate markers for the purpose of tick control.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"171-179"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of vitamin C and early-age thermal conditioning on pituitary adrenocorticotropic hormone cells in broilers chronically exposed to heat stress: an immunohistomorphometric and hormonal study. 维生素 C 和早期热调节对长期暴露于热应激的肉鸡垂体促肾上腺皮质激素细胞的影响:免疫组织形态学和激素研究。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2009320.3981
Jovana Čukuranović Kokoris, Zoran Ružić, Zdenko Kanački, Slobodan Stojanović, Smiljana Paraš, Verica Milošević
{"title":"Effects of vitamin C and early-age thermal conditioning on pituitary adrenocorticotropic hormone cells in broilers chronically exposed to heat stress: an immunohistomorphometric and hormonal study.","authors":"Jovana Čukuranović Kokoris, Zoran Ružić, Zdenko Kanački, Slobodan Stojanović, Smiljana Paraš, Verica Milošević","doi":"10.30466/vrf.2023.2009320.3981","DOIUrl":"10.30466/vrf.2023.2009320.3981","url":null,"abstract":"<p><p>The aim of this study was to examine the effects of heat stress (HS) on the pituitary-adrenal axis and whether the treatments with early-age thermal conditioning (ETC) and vitamin C, alone and in combination, could have a beneficial effect in alleviating these effects. For the experiment, 400 one day-old broilers (both sexes) were used, being divided into four groups. The first group was the control (K), the second group (C) consisted of broilers which received vitamin C from the 22<sup>nd</sup> to the 42<sup>nd</sup> day <i>via</i> water in the amount of 2.00 g L<sup>-1</sup>, in the third group (T), broilers were exposed to ETC for a period of 24 hr at a temperature of 38.00 ± 1.00 ˚C and the fourth group (T + C) was the combination of T and C groups. Immunohistochemically positive adrenocorticotropic hormone (ACTH) cells of broilers in all groups were irregular or stellate and distributed in the periphery and central parts of the pituitary gland, as solitary cells or in clusters. In the T + C group of broilers, a significant increase in the area of ACTH cells (18.91%) and their cores (22.75%), and cortisol level in serum compared to the control group was observed. This reaction of broilers in the T + C group facilitated their adaptation to unfavorable consequences of HS. These results suggest that hypothalamic-pituitary-adrenal axis is stimulated after the exposure to chronic HS, enabling successful adaptation of broilers to adverse conditions.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"125-130"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effect of nanocurcumin on acetaminophen-induced hepatic and renal toxicities in pigeons. 纳米姜黄素对对乙酰氨基酚引起的鸽子肝肾毒性的保护作用
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2024.2011585.4009
Saeed Seifi, Nasser Vajdi, Shohreh Alian Samakkhah, Navideh Mirzakhani
{"title":"Protective effect of nanocurcumin on acetaminophen-induced hepatic and renal toxicities in pigeons.","authors":"Saeed Seifi, Nasser Vajdi, Shohreh Alian Samakkhah, Navideh Mirzakhani","doi":"10.30466/vrf.2024.2011585.4009","DOIUrl":"10.30466/vrf.2024.2011585.4009","url":null,"abstract":"<p><p>In this study, the effects of nanocurcumin on acetaminophen-induced acute hepatorenal toxicity in domestic pigeons (<i>Columba livia</i>) were investigated. Fifteen pigeons were randomly assigned into three groups. Group I was served as a negative control group and received tap water as a placebo. Pigeons in groups II and III were administered acetaminophen at the beginning of the experiment (hr 0). Group III was further treated with nanocurcumin, at 12 hr after acetaminophen administration, being continued every 12 hr for two days. The birds were observed for clinical signs of acute drug toxicity. Blood samples were collected from the pigeons at hr 0, 12, 24 and 48 of the experiment for biochemical analysis of the serum. The results showed that acetaminophen toxicity increased the serum levels of aspartate aminotransferase, alanine aminotransferase, urea and uric acid in the pigeons. Nanocurcumin treatment of acetaminophen intoxicated pigeons attenuated increases in biomarkers of the liver and kidney functions towards control levels. Also, the consumption of nanocurcumin minimized histopathological changes in the liver and kidney. A mortality of 60.00% was seen in the acetaminophen-induced toxicity group; while, none of the birds treated with nanocurcumin died. It can be concluded that nanocurcumin alleviates the acetaminophen-induced acute toxic liver and kidney damages, which can lead to pigeon mortality.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"145-150"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultra-structural organization of the gallbladder mucous membrane of Anglo-Nubian goat. 盎格鲁努比亚山羊胆囊粘膜的超结构组织。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2024.2011081.4005
Anna Valerievna Prusakova, Nikolay Vyacheslavovich Zelenevskiy, Aleksey Viktorovich Prusakov, Anatoly Viktorovich Yashin, Vladimir Sergeevich Ponamarev
{"title":"Ultra-structural organization of the gallbladder mucous membrane of Anglo-Nubian goat.","authors":"Anna Valerievna Prusakova, Nikolay Vyacheslavovich Zelenevskiy, Aleksey Viktorovich Prusakov, Anatoly Viktorovich Yashin, Vladimir Sergeevich Ponamarev","doi":"10.30466/vrf.2024.2011081.4005","DOIUrl":"10.30466/vrf.2024.2011081.4005","url":null,"abstract":"<p><p>Currently, studies devoted to establish the anatomical and histological patterns of the internal organs organization in animals depending on their species and breed, as well as conditions of detention are the most relevant. The liver morphology in representatives of the ruminant family has not been sufficiently studied. Questions regarding the micro- and ultra-structural organizations of the gallbladder wall remain open. The aim of the study was to establish the ultra-structural organization features of the gallbladder mucous membrane of an Anglo-Nubian goat. The material for the study was the gallbladder wall's fragments of an adult Anglo-Nubian goat. Further processing of the obtained samples was carried out to acquire histological preparations. Ultra-thin sections were photographed in a Jem-1011 electron microscope at magnifications of 2,500 - 3,000. It was found that the gallbladder mucous membrane of an Anglo-Nubian goat is formed by the epithelial layer and its own lamina. The epithelial layer is represented by a single-layer prismatic epithelium. The nuclei are light, with clear contours. The apical surface of epithelial cells forms microvilli and the cytoplasm of the apical pole of cells contains many electron-dense secretory granules. The lateral surfaces of the cells in their apical part are interconnected by tight contacts. The lamina propria is formed by loose connective tissue containing many blood vessels and nerve fibers. Referring to the scientific literature describing possible pathologies of the gallbladder, we can conclude that the picture presented in the results generally corresponds to the position of the gallbladder without pathologies.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"165-169"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clonal dispersion and pathogenic potential of multidrug-resistant Aeromonas spp. isolated from Oncorhynchus mykiss with hemorrhagic septicemia. 从鳟鱼出血性败血症中分离出的耐多药气单胞菌的克隆扩散和致病潜力。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2010315.3998
Shaaban Tayar Sadiq, Alaa Hussain Ali Al-Hamdani, Zanan Mohamed-Ameen Taha
{"title":"Clonal dispersion and pathogenic potential of multidrug-resistant <i>Aeromonas</i> spp. isolated from <i>Oncorhynchus mykiss</i> with hemorrhagic septicemia.","authors":"Shaaban Tayar Sadiq, Alaa Hussain Ali Al-Hamdani, Zanan Mohamed-Ameen Taha","doi":"10.30466/vrf.2024.2010315.3998","DOIUrl":"10.30466/vrf.2024.2010315.3998","url":null,"abstract":"<p><p>This study was important to improve proper biosecurity measures and controlling the spread of <i>Aeromonas</i> to prevent future outbreaks. This research sought to determine whether virulent <i>Aeromans</i> species were present in morbid rainbow trout, their resistance and their genetic relatedness. A total number of 542 tissue lesion specimens were collected from gill, liver, heart and kidneys in morbid domesticated fish in Duhok province, Iraq. The <i>gyrB</i> DNA sequence analysis was used to determine the species classification. Drug susceptibility testing was conducted for all isolated strains using disc diffusion technique. The genotyping analysis was carried out using enterobacterial repetitive intergenic consensus-polymerase chain reaction. Thirty-four isolates were found and they were classified into three species (<i>Aeromonas veronii, Aeromonas sorbia,</i> and <i>Aeromonas allosaccharophila)</i>, where <i>A. veronii</i> stand as one of the most prevalent species. The most frequently affected organ by <i>Aeromonas</i> was the gills among four different organs. The detection frequencies of the virulence genes <i>aerolysin,</i> <i>outer membrane protein, glycerophospholipid-cholesterol acyltransferase, elastase, flagella,</i> <i>serine protease, cytotonic heat-</i>labile<i>,</i> and <i>hemolysin</i> were 100%, 100%, 79.41%, 64.70%, 76.47%, 67.64%, 70.58%, and 41.17, respectively. None of the strains possessed all of the virulence markers. All isolates were completely resistant to ceftazidime, amoxicillin and doxycycline. All isolates were found to be multi-drug-resistant. Regardless of the nearest geographic source area of samples and the same <i>Aeromonas</i> species, there was a high genetic diversity. The results of this study could help farmers and researchers make informed decisions about measures of biosecurity and proper therapeutic drugs to apply to prevent current outbreaks and prevent them from recurring again.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"529-536"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model. Mito-TEMPO对小鼠睾丸扭转和扭转模型缺血再灌注损伤的保护作用。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2024815.4202
Zohreh Mostahsan, Saeed Azizi, Ali Soleimanzadeh, Ali Shalizar-Jalali
{"title":"Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.","authors":"Zohreh Mostahsan, Saeed Azizi, Ali Soleimanzadeh, Ali Shalizar-Jalali","doi":"10.30466/vrf.2024.2024815.4202","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024815.4202","url":null,"abstract":"<p><p>Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of unscavenged reactive oxygen species. This study aimed to investigate the effects of intra-peritoneal administration of Mito-TEMPO (MT) on I/R injury in testicular torsion/detorsion (T/D) in mice. Forty-two male mice were divided into seven groups including 1 control and 6 treatment groups (360° T/D, 720° T/D, 360° T/D + 0.70 mg kg<sup>-1</sup> MT, 360° T/D + 1.00 mg kg<sup>-1</sup> MT, 720° T/D + 0.70 mg kg<sup>-1</sup> MT, and 720° T/D + 1.00 mg kg<sup>-1</sup> MT). After inducing 360° and 720° clockwise testicular torsions for 2 hr, sperm parameters, apoptosis-related genes expression, and <i>in vivo</i> fertility index were evaluated. The results showed that 720° T/D can lead to increased abnormal sperm morphology, sperm DNA damage, and <i>Bax</i> expression, while the <i>Bcl-2</i> expression was reduced compared to the other groups. In addition, it also had negative effects on sperm total and progressive motilities as well as viability and plasma membrane functionality (PMF). The results also showed that administration of MT to T/D mice can result in a reduction in abnormal sperm morphology, DNA damage, and <i>Bax</i> expression. It could also increase sperm total and progressive motilities, viability and PMF, <i>Bcl-2</i> expression, and <i>in vivo</i> fertility index. Based on our results, it is concluded that MT, when administered after spermatic cord torsion in mice, provides significant protection against acute testicular T/D injury.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"665-672"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viability of guinea pig (Cavia porcellus) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C. 用Tris-buffer扩展器在5.00℃下保存的豚鼠(Cavia porcellus)精子的活力。
IF 0.8 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2023686.4186
Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad
{"title":"Viability of guinea pig (<i>Cavia porcellus</i>) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C.","authors":"Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad","doi":"10.30466/vrf.2024.2023686.4186","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023686.4186","url":null,"abstract":"<p><p>The cooling procedure markedly diminishes the quality of guinea pig (<i>Cavia porcellus</i>) sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions. This study aimed to assess the viability of guinea pig spermatozoa diluted in Tris buffer-based extenders during storage at 5.00 ˚C. Sperm collection was carried out through castration of the animals. For this study, 10 adult male guinea pigs were utilized, being divided into four groups including phosphate-buffered saline (PBS), human tubal fluid (HTF), Tris-citric-glucose (TCG), and Tris-fructose-yolk (TFY) cultures. Evaluations including sperm motility, morphology, plasma membrane integrity, viability, and total count were conducted at 0, 24, and 48 hr after sampling. The results obtained indicated that at the 24-hr and 48-hr marks of the experiment, both overall and progressive motility percentages, viability, plasma membrane integrity, and morphology of sperms in the PBS and HTF cultures exhibited a significant increase in comparison with the TCG and TFY cultures. Consequently, it can be inferred that PBS and HTF cultures are highly effective in preserving the quality of guinea pig spermatozoa.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"691-696"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. 比较乙酰丙嗪-氯硝柳胺与乙酰丙嗪-氯硝柳胺复方制剂对马的镇静和心血管作用。
IF 1 4区 农林科学
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2004451.3910
Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei
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