Veterinary Research Forum最新文献_第2页

First report of histopathological and molecular characterizations of bovine herpesvirus-1 from outbreak at dairy farm in India. 印度奶牛场暴发的牛疱疹病毒-1的组织病理学和分子特征的首次报告。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2028036.4260

Amit Kumar Verma, Vikas Jaiswal, Arbind Singh, Ramakant, Vishnu Kumar Rai

{"title":"First report of histopathological and molecular characterizations of bovine herpesvirus-1 from outbreak at dairy farm in India.","authors":"Amit Kumar Verma, Vikas Jaiswal, Arbind Singh, Ramakant, Vishnu Kumar Rai","doi":"10.30466/vrf.2024.2028036.4260","DOIUrl":"10.30466/vrf.2024.2028036.4260","url":null,"abstract":"Infectious bovine rhinotracheitis, caused by bovine herpesvirus-1 (BHV-1) is a highly contagious viral disease affecting bovines, and clinically characterized by pyrexia, inappetence, respiratory distress, dyspnoea, conjunctivitis, nasal discharge, and sometimes abortions. In the present study, buffalo dairy farm having high mortality was investigated. The buffaloes were suffering from high rectal temperature, conjunctivitis, severe respiratory distress, and nasal discharge. Tissue samples from upper respiratory tract were collected aseptically following post-mortem examination of died buffaloes. Tracheal tissue samples were then processed for histopathological examination and DNA isolation. The presence of BHV-1 in the tissue samples was confirmed by nested polymerase chain reaction using glycoprotein B gene primers. The present study reported for the first time the clinical signs, post-mortem lesions, histopathological evidence, and detection of DNA of BHV-1 glycoprotein B gene through nested polymerase chain reaction assay during an active outbreak in buffaloes in India. The findings of this study are crucial for improving the diagnosis of BHV-1 and ultimately reducing financial losses within dairy industry.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"301-304"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bioactive peptides on heat stress-induced testiculopathies in mature rats: immunohistopathological evidence. 生物活性肽对成熟大鼠热应激性睾丸病变的影响：免疫组织病理学证据。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2032033.4318

Mahsa Valizadeh, Vahid Nejati, Ali Shalizar-Jalali, Ebrahim Najdegerami, Gholamreza Najafi

{"title":"Effect of bioactive peptides on heat stress-induced testiculopathies in mature rats: immunohistopathological evidence.","authors":"Mahsa Valizadeh, Vahid Nejati, Ali Shalizar-Jalali, Ebrahim Najdegerami, Gholamreza Najafi","doi":"10.30466/vrf.2024.2032033.4318","DOIUrl":"10.30466/vrf.2024.2032033.4318","url":null,"abstract":"Exposure to extreme temperature conditions such as occurs in certain occupations is known to induce male infertility. In humans and most of the mammals, it has been shown that whole-body heat stress (HS) decreases fertility and produces defective embryos. Hence, the present study aimed at gaining some insights into the mechanisms producing defects after HS. In the present study, 56 mature male Wistar rats were randomly categorized into eight groups (n = 7), including group 1: Control, groups 2: Bioactive peptides (BPs; 10.00 mg kg-1), groups 3, 4, and 5: Heat-stressed (37.00, 39.00, and 43.00 ˚C for 20 min, respectively), and groups 6, 7, and 8: Heat-stressed along with BPs (10.00 mg kg-1), respectively. All treatments were administered orally once per day. The HS was induced through the immersion of rat scrotums in a water bath. After 45 days, rats were sacrificed and left testes were removed, fixed, and used for histological and immunohistochemical studies. Harvested right testes were also used for oxidative stress assessments and molecular analyses. Heat stress increased testicular tissue damage, elevated oxidative stress and reactive oxygen species production, and increased germ cells apoptosis, p53 and caspase 3 expressions, and Bax/Bcl-2 ratio. Treatment with BPs as a substance with anti-oxidant properties ameliorated the damage caused by HS. The results of this study highlight the protective role of BPs in the reproductive tract under HS. Bioactive peptides may have potential function against testicular tissue oxidative stress and apoptosis.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"106-116"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective effect of royal jelly on dibutyl phthalate-induced liver injury in rats. 蜂王浆对邻苯二甲酸二丁酯所致大鼠肝损伤的保护作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2033878.4338

Mahdieh Nezami Majd, Goudarz Sadeghi-Hashjin, Hassan Malekinejad, Ali Rassouli

{"title":"Hepatoprotective effect of royal jelly on dibutyl phthalate-induced liver injury in rats.","authors":"Mahdieh Nezami Majd, Goudarz Sadeghi-Hashjin, Hassan Malekinejad, Ali Rassouli","doi":"10.30466/vrf.2024.2033878.4338","DOIUrl":"10.30466/vrf.2024.2033878.4338","url":null,"abstract":"Phthalate esters, such as dibutyl phthalate (DBP), are extensively utilized and human and animal exposure leads to serious toxic effects, including hepatotoxicity. In the present study the protective effects of royal jelly (RJ) on DBP-induced liver damage was investigated. A total number of 40 Wistar albino rats were randomly divided into eight groups (n = 5): control (corn oil), DBP (500 mg kg-1), RJ (200 mg kg1), Quercetin (QCN; 50.00 mg kg-1), RJ (100 mg kg-1) + DBP, RJ (200 mg kg-1) + DBP, RJ (300 mg kg-1) + DBP, QCN (50.00 mg kg-1) + DBP. After 28 days of daily oral gavage treatment, animals were euthanized. The insulin resistance index, lipid profile and hepatic enzymes were measured on the collected serum samples. Moreover, oxidative and nitrosative stress biomarkers were determined in the liver. Histopathological alterations and ultimately cytochrome P450 2E1 (CYP2E1) activity was also assessed. Data obtained revealed that RJ significantly reduced the insulin resistance index and liver enzymes level in RJ-DBP groups. At the same time, RJ recovered the DBP-induced oxidative stress and restored the DBP-depleted glutathione. Moreover, RJ improved lipid profile and reduced significantly the DBP-induced hepatic CYP 2E1 activity in RJ-DBP groups. Dibutyl phthalate induced-hepatic damage such as necrosis of hepatocytes and scattered bleeding was alleviated in RJ-DBP group. Our data suggested that the administration of RJ could protect the DBP-induced hepatic functional and structural alterations. The RJ protective effects might be attributed to its antioxidant and anti-inflammatory properties and reduced CYP 2E1 activity.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"97-105"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture. 胫骨平台平截骨：基质金属蛋白酶在颅十字韧带断裂后犬膝关节稳定长期监测中的意义。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2023.2017099.4089

Dragan Jevrem Ristanović, Jelena Vladimir Francuski Andrić, Milena Danilo Radaković, Kristina Adam Spariosu, Ivan Željko Vlahek, Marija Tomo Lipar, Petar Sreten Milosavljević, Mario Ivan Kreszinger

{"title":"Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture.","authors":"Dragan Jevrem Ristanović, Jelena Vladimir Francuski Andrić, Milena Danilo Radaković, Kristina Adam Spariosu, Ivan Željko Vlahek, Marija Tomo Lipar, Petar Sreten Milosavljević, Mario Ivan Kreszinger","doi":"10.30466/vrf.2023.2017099.4089","DOIUrl":"10.30466/vrf.2023.2017099.4089","url":null,"abstract":"Cranial cruciate ligament rupture is one of the most common causes of osteoarthritis in dogs. Surgical stabilization is obligatory and tibial plateau levelling osteotomy (TPLO) is the most commonly used surgical technique. Studies on the long-term monitoring of matrix metallo-proteinases (MMPs) and acute-phase proteins are limited, especially those with parallel monitoring of changes in the serum, synovial fluid (SF) and urine. We aimed to describe long-term changes in 1) MMPs: gelatinases and caseinases, 2) APPs: ceruloplasmin, haptoglobin and paraoxonase-1, and 3) the correlation of MMPs in the serum, SF and urine with lameness in dogs 2 and 6 months undergoing TPLO. From 17 dogs diagnosed with cranial cruciate ligament rupture, sera, SF and urine samples were collected preoperative 2 and 6 months after the surgery. Relative activity (RA) of MMPs was measured in all samples using zymography. Acute-phase proteins were measured in the serum and SF using spectrophotometry and agarose gel electrophoresis. Relative activity of MMPs in serum was not change at different sampling points. In SF, a marked decrease in MMPs RA was evident, however, only RA of caseinases was significantly reduced during the recovery period. In urine, RA of caseinases was positively correlated with the lameness score with decreasing activity trend during time. Serum and synovial acute-phase proteins were not changed after surgery, which was consistent with the agarose gel analyses. Decreased activity of caseinases pointed to reduced degradation of extracellular matrix after TPLO. Synovial and urine caseinases are potential biomarkers in predicting the recovery outcome following stifle stabilization.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"63-69"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain. 禽传染性支气管炎病毒M41株接种特异性无病原体卵胚中气管、肺和中肾的组织病理学评价。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2032492.4322

Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi

{"title":"Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain.","authors":"Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi","doi":"10.30466/vrf.2024.2032492.4322","DOIUrl":"10.30466/vrf.2024.2032492.4322","url":null,"abstract":"Infectious bronchitis, being caused by a coronavirus, is a significant disease affecting broiler and layer chickens, leading to substantial losses in the poultry industry due to the high mortality rates and decreased egg yield. Nearly 30 serotypes and 100 variants were described to date; developed vaccines are being for some severe cases, like the Massachusetts strain, to mitigate the effects. Determining the vaccinal strain's titer is crucial for creating an effective vaccine, and calculating the virus infectivity in the egg embryo is very important using dilutions ranging from 10-3 to 10-8, from each dilution 0.10 mL is used. The aim of this study was to determine the effects of the avian bronchitis virus injected into the allantoic cavity of ten days old embryonated eggs. Real-time polymerase chain reaction tests determined the viral load in the allantoic fluid. The embryos were removed to study gross injuries. The trachea, lung, and mesonephros were removed and submitted for histopathological studies, and nuclear factor-kappa B immunofluorescence analysis. The results revealed that the dilution of one-thousandth of the virus in the embryos caused the highest organ damage and viral replication. Varying degrees of hyperemia, edema, cellular infiltration, and degeneration were observed in the trachea, lung, and mesonephros depending on the virus dilution. This study provides valuable insights into the pathogenesis of the avian bronchitis virus, and has a potential impact on achieving an effective vaccine.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"161-166"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating serological evidence of Schmallenberg virus in cattle in eastern Algeria. 调查阿尔及利亚东部牛中存在施马伦贝格病毒的血清学证据。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2026853.4238

Nadia Djellata, Abdelkrim Yahimi, Christian Hanzen

{"title":"Investigating serological evidence of Schmallenberg virus in cattle in eastern Algeria.","authors":"Nadia Djellata, Abdelkrim Yahimi, Christian Hanzen","doi":"10.30466/vrf.2024.2026853.4238","DOIUrl":"10.30466/vrf.2024.2026853.4238","url":null,"abstract":"Schmallenberg virus (SBV) is a newly identified virus belonging to the Orthobunyavirus genus, of the Bunyaviridae family, and transmitted by haematophagous arthropods in particular mosquitoes and biting midges of the Culicoides genus. The SBV is known to cause reproductive disorders in ruminants mainly abortions, stillbirths and congenital malformations (hydranencephaly and arthrogryposis syndromes). The aim of this study was to investigate the presence of SBV in dairy cattle in Algeria. Between September 2023 and December 2023, blood serum samples from 300 dairy cows from 75 dairy farms in north eastern Algeria were tested for SBV antibodies using a commercial indirect enzyme-linked immunosorbent assay kit. Individual seroprevalence was 38.33% (115/300; 95.00% confidence interval: 32.83 - 43.83), while herd seroprevalence was 41.33% (31/75; 95.00% confidence interval: 30.18 - 52.47). In addition, the results of this study revealed that SBV seroprevalence at individual or herd level was high and not negligible confirming the presence of SBV in the regions studied in Algeria. In conclusion, more in-depth studies are recommended concerning the molecular proof, origin and pathogenesis of SBV in ruminants mainly those linked to reproductive disorders as well as the study of the various associated risk factors.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"129-132"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of bovine group A rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium spp., Giardia spp. in calf diarrhea with one-step duplex RT-PCR: histopathological and immunochromotographic methods. 牛A组轮状病毒、牛冠状病毒、大肠杆菌K99、隐孢子虫、贾第鞭毛虫腹泻的一步双工RT-PCR诊断：组织病理学和免疫色谱法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2025299.4208

Bunyamin Irehan, Mustafa Ozkaraca, Irem Gulactı, Levent Sener, Muhammed Fatih Dursun

{"title":"Diagnosis of bovine group A rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium spp., Giardia spp. in calf diarrhea with one-step duplex RT-PCR: histopathological and immunochromotographic methods.","authors":"Bunyamin Irehan, Mustafa Ozkaraca, Irem Gulactı, Levent Sener, Muhammed Fatih Dursun","doi":"10.30466/vrf.2024.2025299.4208","DOIUrl":"10.30466/vrf.2024.2025299.4208","url":null,"abstract":"Infections causing diarrhea in cattle breeding pose a substantial threat to calf breeding and health, causing considerable economic losses worldwide, including in Türkiye. The primary causative agents of calf diarrhea include bovine group A rotavirus, bovine coronavirus (BCoV), Escherichia coli K99, Cryptosporidium spp., and Giardia spp. The objective of this study was to diagnose the pathogens present in samples taken from calves exhibiting diarrhea symptoms in the Elaziğ province of Türkiye. The study material consisted of 85 fecal samples from calves up to 30 days of age with diarrhea symptoms and 42 small intestine samples from calves died as a result of diarrhea symptoms after necropsy. Following total RNA isolation from the samples, one-step duplex reverse transcription polymerase chain reaction (RT-PCR) analysis was conducted to identify the bovine rotavirus (BRV) and BCoV. Our results indicated that 24 of the samples were positive for BRV, 13 were positive for BCoV, and 25 were positive for BRV and BCoV in the form of mixed infection. The immunochromatographic analysis revealed that three samples were positive for E. coli K99 antigens, 12 for Cryptosporidium spp. antigens, and 28 for Giardia spp. antigens. Our results indicate that one-step duplex RT-PCR, immunohistochemistry, and immunochromatography methods can be valuable tests to be used in routine diagnostic laboratories for the detection of pathogens associated with calf diarrhea.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"221-227"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats. 维生素原A类胡萝卜素（β-隐黄质）改善成熟大鼠睾丸缺血再灌注损伤。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2025.2046637.4559

Kianoush Mohammadnehjad, Rahim Mohammadi, Ali Soleimanzadeh, Ali Shalizar-Jalali, Farshid Sarrafzadeh-Rezaei

{"title":"Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats.","authors":"Kianoush Mohammadnehjad, Rahim Mohammadi, Ali Soleimanzadeh, Ali Shalizar-Jalali, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2025.2046637.4559","DOIUrl":"10.30466/vrf.2025.2046637.4559","url":null,"abstract":"Buildup of reactive oxygen species during testicular torsion causes oxidative stress and ischemia-reperfusion (I/R) injury in testis. The purpose of this study was to investigate influence of β-cryptoxanthin (BCX) on I/R injury in testicular torsion/detorsion in mature rats. Thirty mature male Wistar rats were divided into five groups of six animals each, including sham group: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hr with a 720-degree rotation, I/R group: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hr with a 720-degree rotation, I/R/Oil group: In this group, a midline scrotum cut was performed, the testicles were taken out, ischemia was created for 2 hr with a 720-degree rotation, and at the end of ischemia 100 µL of corn oil (BCX solvent) was injected intraperitoneally, I/R/BCX10 group: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (10.00 µg kg-1) at the end of ischemia, and I/R/BCX40: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (40.00 µg kg-1) at the end of ischemia. Evaluations were based on histopathological and spermatological parameters and oxidative stress assessments. Histopathological spermatological and oxidative stress parameters values obtained from I/R/BCX40 were significantly different from those of other groups (p < 0.05). It could be concluded that BCX could ameliorate testicular injuries in acute testicular torsion/detorsion in mature rats.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"277-284"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of olive oil against cardiac aging through mitophagy and apoptosis. 橄榄油通过有丝分裂和细胞凋亡对心脏衰老的保护作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030624.4304

Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari

{"title":"Protective effects of olive oil against cardiac aging through mitophagy and apoptosis.","authors":"Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari","doi":"10.30466/vrf.2024.2030624.4304","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030624.4304","url":null,"abstract":"Cardiac mitochondrial dysfunction is an important feature of aged heart. However, there is still no potent agent to ameliorate cardiac function abnormalities in aged hosts. Olive oil (OLO), containing monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-diabetic, anti-inflammatory, and anti-hypertensive effects. We evaluated the beneficial impacts of OLO against aging-related cardiac dysfunction. Wistar rats were randomly allotted into three groups with eight rats, including control, aged rats receiving D-galactose (D-GAL), and aged rats administrated with D-galactose plus OLO (D-GAL + OLO). Aged animals were received D-GAL at a dose of 150.00 mg kg-1 daily through intra-peritoneal injection for aging induction. The animals in D-GAL + OLO group were co-administrated with oral OLO at a dose of 1.00 mL kg-1 by gavage feeding daily. The administration term was eight weeks. A histological examination of heart tissue was performed. The heart tissues were also harvested to assay the oxidative stress and molecular parameters. The aged animals showed cardiac hypertrophy, increased malondialdehyde level and Bax expression, and reduced mitofusin 2, phosphatase and tensin homologue-induced putative kinase 1, dynamin-related protein 1, and Bcl2 expressions in comparison with the control animals. The OLO treatment ameliorated all these parameters. Overall, OLO could improve cardiac aging through reducing oxidative stress, enhancing genes mediated mitophagy, and improving genes mediated apoptosis in the heart.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"27-33"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination and comparison of volatile compounds of different poultry species eggs. 不同禽种鸡蛋挥发性成分的测定与比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2022511.4164

Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz

{"title":"Determination and comparison of volatile compounds of different poultry species eggs.","authors":"Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz","doi":"10.30466/vrf.2024.2022511.4164","DOIUrl":"10.30466/vrf.2024.2022511.4164","url":null,"abstract":"All fresh poultry eggs differ greatly in chemical components. Many chemicals have been investigated in this field, but the presence of differences in odor components between species has not yet been investigated. For this reason, this study aimed to determine the volatile compounds of the eight different poultry species eggs and the differences among them. In this context, 180 eggs (20 from each species) were purchased from different farms, and the volatile compounds of raw eggs were determined by the solid phase micro-extraction method. Following the analysis, 41 volatile compounds and 12 organic groups were identified. The variety and ratios of these compounds varied among the species, with pigeon eggs containing the greatest number of compounds (19 compounds) and quail, partridge, and pheasant eggs containing the lowest numbers (seven compounds). Acetamide, 2-fluoro-, and D-limonene compounds were found in every species of eggs. The other species eggs did not contain any of the 10 volatile chemicals detected in pigeon eggs, eight in chicken eggs, and one in quail eggs. It was discovered that the eggs volatile compounds counts and chemical profiles varied, indicating differences in their tastes, smells, and aromas. The volatile chemicals found in this study may be species-specific and can be used as indicators to identify which eggs belong to which species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"149-159"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0