Veterinary Research Forum最新文献_第2页

Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain. 禽传染性支气管炎病毒M41株接种特异性无病原体卵胚中气管、肺和中肾的组织病理学评价。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2032492.4322

Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi

{"title":"Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain.","authors":"Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi","doi":"10.30466/vrf.2024.2032492.4322","DOIUrl":"10.30466/vrf.2024.2032492.4322","url":null,"abstract":"Infectious bronchitis, being caused by a coronavirus, is a significant disease affecting broiler and layer chickens, leading to substantial losses in the poultry industry due to the high mortality rates and decreased egg yield. Nearly 30 serotypes and 100 variants were described to date; developed vaccines are being for some severe cases, like the Massachusetts strain, to mitigate the effects. Determining the vaccinal strain's titer is crucial for creating an effective vaccine, and calculating the virus infectivity in the egg embryo is very important using dilutions ranging from 10-3 to 10-8, from each dilution 0.10 mL is used. The aim of this study was to determine the effects of the avian bronchitis virus injected into the allantoic cavity of ten days old embryonated eggs. Real-time polymerase chain reaction tests determined the viral load in the allantoic fluid. The embryos were removed to study gross injuries. The trachea, lung, and mesonephros were removed and submitted for histopathological studies, and nuclear factor-kappa B immunofluorescence analysis. The results revealed that the dilution of one-thousandth of the virus in the embryos caused the highest organ damage and viral replication. Varying degrees of hyperemia, edema, cellular infiltration, and degeneration were observed in the trachea, lung, and mesonephros depending on the virus dilution. This study provides valuable insights into the pathogenesis of the avian bronchitis virus, and has a potential impact on achieving an effective vaccine.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"161-166"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating serological evidence of Schmallenberg virus in cattle in eastern Algeria. 调查阿尔及利亚东部牛中存在施马伦贝格病毒的血清学证据。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2026853.4238

Nadia Djellata, Abdelkrim Yahimi, Christian Hanzen

{"title":"Investigating serological evidence of Schmallenberg virus in cattle in eastern Algeria.","authors":"Nadia Djellata, Abdelkrim Yahimi, Christian Hanzen","doi":"10.30466/vrf.2024.2026853.4238","DOIUrl":"10.30466/vrf.2024.2026853.4238","url":null,"abstract":"Schmallenberg virus (SBV) is a newly identified virus belonging to the Orthobunyavirus genus, of the Bunyaviridae family, and transmitted by haematophagous arthropods in particular mosquitoes and biting midges of the Culicoides genus. The SBV is known to cause reproductive disorders in ruminants mainly abortions, stillbirths and congenital malformations (hydranencephaly and arthrogryposis syndromes). The aim of this study was to investigate the presence of SBV in dairy cattle in Algeria. Between September 2023 and December 2023, blood serum samples from 300 dairy cows from 75 dairy farms in north eastern Algeria were tested for SBV antibodies using a commercial indirect enzyme-linked immunosorbent assay kit. Individual seroprevalence was 38.33% (115/300; 95.00% confidence interval: 32.83 - 43.83), while herd seroprevalence was 41.33% (31/75; 95.00% confidence interval: 30.18 - 52.47). In addition, the results of this study revealed that SBV seroprevalence at individual or herd level was high and not negligible confirming the presence of SBV in the regions studied in Algeria. In conclusion, more in-depth studies are recommended concerning the molecular proof, origin and pathogenesis of SBV in ruminants mainly those linked to reproductive disorders as well as the study of the various associated risk factors.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"129-132"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of bovine group A rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium spp., Giardia spp. in calf diarrhea with one-step duplex RT-PCR: histopathological and immunochromotographic methods. 牛A组轮状病毒、牛冠状病毒、大肠杆菌K99、隐孢子虫、贾第鞭毛虫腹泻的一步双工RT-PCR诊断：组织病理学和免疫色谱法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2025299.4208

Bunyamin Irehan, Mustafa Ozkaraca, Irem Gulactı, Levent Sener, Muhammed Fatih Dursun

{"title":"Diagnosis of bovine group A rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium spp., Giardia spp. in calf diarrhea with one-step duplex RT-PCR: histopathological and immunochromotographic methods.","authors":"Bunyamin Irehan, Mustafa Ozkaraca, Irem Gulactı, Levent Sener, Muhammed Fatih Dursun","doi":"10.30466/vrf.2024.2025299.4208","DOIUrl":"10.30466/vrf.2024.2025299.4208","url":null,"abstract":"Infections causing diarrhea in cattle breeding pose a substantial threat to calf breeding and health, causing considerable economic losses worldwide, including in Türkiye. The primary causative agents of calf diarrhea include bovine group A rotavirus, bovine coronavirus (BCoV), Escherichia coli K99, Cryptosporidium spp., and Giardia spp. The objective of this study was to diagnose the pathogens present in samples taken from calves exhibiting diarrhea symptoms in the Elaziğ province of Türkiye. The study material consisted of 85 fecal samples from calves up to 30 days of age with diarrhea symptoms and 42 small intestine samples from calves died as a result of diarrhea symptoms after necropsy. Following total RNA isolation from the samples, one-step duplex reverse transcription polymerase chain reaction (RT-PCR) analysis was conducted to identify the bovine rotavirus (BRV) and BCoV. Our results indicated that 24 of the samples were positive for BRV, 13 were positive for BCoV, and 25 were positive for BRV and BCoV in the form of mixed infection. The immunochromatographic analysis revealed that three samples were positive for E. coli K99 antigens, 12 for Cryptosporidium spp. antigens, and 28 for Giardia spp. antigens. Our results indicate that one-step duplex RT-PCR, immunohistochemistry, and immunochromatography methods can be valuable tests to be used in routine diagnostic laboratories for the detection of pathogens associated with calf diarrhea.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"221-227"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of olive oil against cardiac aging through mitophagy and apoptosis. 橄榄油通过有丝分裂和细胞凋亡对心脏衰老的保护作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030624.4304

Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari

{"title":"Protective effects of olive oil against cardiac aging through mitophagy and apoptosis.","authors":"Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari","doi":"10.30466/vrf.2024.2030624.4304","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030624.4304","url":null,"abstract":"Cardiac mitochondrial dysfunction is an important feature of aged heart. However, there is still no potent agent to ameliorate cardiac function abnormalities in aged hosts. Olive oil (OLO), containing monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-diabetic, anti-inflammatory, and anti-hypertensive effects. We evaluated the beneficial impacts of OLO against aging-related cardiac dysfunction. Wistar rats were randomly allotted into three groups with eight rats, including control, aged rats receiving D-galactose (D-GAL), and aged rats administrated with D-galactose plus OLO (D-GAL + OLO). Aged animals were received D-GAL at a dose of 150.00 mg kg-1 daily through intra-peritoneal injection for aging induction. The animals in D-GAL + OLO group were co-administrated with oral OLO at a dose of 1.00 mL kg-1 by gavage feeding daily. The administration term was eight weeks. A histological examination of heart tissue was performed. The heart tissues were also harvested to assay the oxidative stress and molecular parameters. The aged animals showed cardiac hypertrophy, increased malondialdehyde level and Bax expression, and reduced mitofusin 2, phosphatase and tensin homologue-induced putative kinase 1, dynamin-related protein 1, and Bcl2 expressions in comparison with the control animals. The OLO treatment ameliorated all these parameters. Overall, OLO could improve cardiac aging through reducing oxidative stress, enhancing genes mediated mitophagy, and improving genes mediated apoptosis in the heart.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"27-33"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination and comparison of volatile compounds of different poultry species eggs. 不同禽种鸡蛋挥发性成分的测定与比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2022511.4164

Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz

{"title":"Determination and comparison of volatile compounds of different poultry species eggs.","authors":"Fatma Yenilmez, Ozgul Anitas, Serap Goncu, Nurten Yilmaz","doi":"10.30466/vrf.2024.2022511.4164","DOIUrl":"10.30466/vrf.2024.2022511.4164","url":null,"abstract":"All fresh poultry eggs differ greatly in chemical components. Many chemicals have been investigated in this field, but the presence of differences in odor components between species has not yet been investigated. For this reason, this study aimed to determine the volatile compounds of the eight different poultry species eggs and the differences among them. In this context, 180 eggs (20 from each species) were purchased from different farms, and the volatile compounds of raw eggs were determined by the solid phase micro-extraction method. Following the analysis, 41 volatile compounds and 12 organic groups were identified. The variety and ratios of these compounds varied among the species, with pigeon eggs containing the greatest number of compounds (19 compounds) and quail, partridge, and pheasant eggs containing the lowest numbers (seven compounds). Acetamide, 2-fluoro-, and D-limonene compounds were found in every species of eggs. The other species eggs did not contain any of the 10 volatile chemicals detected in pigeon eggs, eight in chicken eggs, and one in quail eggs. It was discovered that the eggs volatile compounds counts and chemical profiles varied, indicating differences in their tastes, smells, and aromas. The volatile chemicals found in this study may be species-specific and can be used as indicators to identify which eggs belong to which species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"149-159"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model. 局部给药氨甲环酸预防术后硬膜外纤维化：来自兔椎板切除术模型的见解。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2031220.4312

Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem

{"title":"Topical administration of tranexamic acid for prevention of postoperative epidural fibrosis: insights from a rabbit laminectomy model.","authors":"Arian Rahmani, Soroush Mohitmafi, Fariborz Moayer, Mohammad Molazem","doi":"10.30466/vrf.2024.2031220.4312","DOIUrl":"10.30466/vrf.2024.2031220.4312","url":null,"abstract":"Significant advancements in imaging and surgical methodologies have led to more frequent performance of neurosurgical procedures such as laminectomy in both animal and human patients. Epidural fibrosis (EF) is defined as the excessive formation of scar tissue in the epidural space after lumbar laminectomy, often resulting in recurring postoperative pain. Given the association between postoperative hematoma accumulation at the laminectomy site and the development of EF, the present study aimed to evaluate the preventive impact of tranexamic acid (TXA), an antifibrinolytic agent with well-recognized hemostatic properties across various surgical fields. A rabbit laminectomy model was constructed to assess its effectiveness in reducing EF formation. A total number of 18 adult New Zealand White male rabbits were randomly divided into two groups: The control (saline) group and the treatment (topical TXA) group. Each rabbit underwent a two-level laminectomy at L3-L4. The treatment group received 5.00 mL of 100 mg mL-1 TXA solution applied topically to the laminectomy site, while the control group received 5.00 mL of saline. Postoperative evaluations included magnetic resonance imaging at week six to assess EF, followed by histopathological examinations to evaluate fibroblast cell density in scar tissue, EF grading and thickness of the dura mater. The analysis of magnetic resonance imaging and histopathologic data revealed significant differences between the two groups indicating that topical administration of TXA might be a promising approach for preventing EF.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"181-187"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology. 揭示多酶鸡尾酒索诺芽孢杆菌BD92对商品肉鸡生长性能和肠道组织学的有益影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2025777.4221

Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir

{"title":"Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology.","authors":"Ahmad Raza, Muhammad Irfan Anwar, Shehbaz Ali, Mian Muhammad Awais, Faqir Muhammad, Habib Ur Rahman, Maryum Rasheed, Syeda Mariam Fatima, Muhammad Hamid Rasheed, Muhammad Kashif Saleemi, Ulas Acaroz, Saira Bashir","doi":"10.30466/vrf.2024.2025777.4221","DOIUrl":"10.30466/vrf.2024.2025777.4221","url":null,"abstract":"Crude fiber (CF) is a vital component in poultry nutrition with a notable phytonutrient effectively indicating the presence of indigestible biomass in food due to the absence of digestive enzymes for CF in broilers. This study aimed to analyze the properties of a multi-enzyme cocktail (MEC) Bacillus sonorensis BD92 (BsBD92) comprised of xylanase, β-glucosidase, exo-glucanase, and endo-glucanase enzymes. Also, this study intended to look at the growth performance and intestinal histology of broilers in the starter and finisher phases by the addition of MEC BsBD92 to their diet. To evaluate the efficacy of MEC BsBD92, 140 one-day-old unsexed Cobb500 broiler chicks were randomly divided into seven groups receiving different diets. The characterization of exo-glucanase, xylanase, β-glucosidase, and endo-glucanase showed that their peak activities were observed at a temperature of 50.00 ˚C and a pH of 5.50. The 6.00% CF and 2.00 X MEC BsBD92 improved the intestinal morphology and feed conversion ratio, demonstrating a synergistic effect on growth performance. Whereas, increasing meat percentages to 61.06 and 65.09 g per 100 g body weight during the starter and finisher phases was also observed, respectively. The lipid profiles revealed significant variations in triglyceride and cholesterol levels. This study provides an innovative approach, considering not only lowering the feed cost using inexpensive fibrous feedstuffs but also improving the feed efficiency through supplementation of MEC BsBD92.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"209-219"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases. N- 乙酰半胱氨酸在治疗犬血液原虫病引起的全血细胞减少症中可增强骨髓活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2033155.4328

Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das

{"title":"N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases.","authors":"Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das","doi":"10.30466/vrf.2024.2033155.4328","DOIUrl":"https://doi.org/10.30466/vrf.2024.2033155.4328","url":null,"abstract":"Canine hemoprotozoan diseases viz. ehrlichiosis and babesiosis are mostly associated with critical anemia and thrombocytopenia with pancytopenic changes, leading to multi-organ failure. For faster recovery of patients with complicated hemoprotozoan diseases, whole blood transfusion or bone marrow stimulating agents to produce more red blood cells (RBCs) and platelets might be helpful. Unfortunately, canine specific transfusion procedures are expensive and even not available in many developing countries. Development of alternate therapeutic modality by bone marrow stimulation to augment the production of RBCs and platelets and thus, to treat the critical pancytopenic patients is and an urgent necessity. N-acetylcysteine (NAC), acts as a precursor of reduced glutathione and increases the production of bone marrow B cells. It also improves viability and self-renewal capacity of stem cells and thus, boosts hematopoietic differentiation by protecting induced pluripotent stem cells. This study envisaged to develop alternate therapeutic approach to combat pancytopenia secondary to canine hemoprotozoan diseases. Bone marrow mediated aplastic pancytopenia was induced experimentally by administration of cyclophosphamide in rats. Bone marrow stimulating property of NAC was compared with desmopressin, another bone marrow stimulator, which revealed better in terms of hematobiochemical and histopathological changes. Results of rat model study were extrapolated in clinical canine hemoprotozoan cases having pancytopenia. Dogs treated with hemoprotozoan disease specific therapy along with NAC rendered favorable changes by haltering the progression of critical anemia and thrombocytopenia. Study revealed that supplementation of NAC along with canine hemoprotozoan specific therapy is beneficial to alleviate pancytopenia.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran. 伊朗养蜂场欧洲臭蝇病主、次病原检测的流行病学研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2026240.4232

Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani

{"title":"Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran.","authors":"Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani","doi":"10.30466/vrf.2024.2026240.4232","DOIUrl":"https://doi.org/10.30466/vrf.2024.2026240.4232","url":null,"abstract":"Infecting to Melissococcus plutonius, the primary cause of the European foulbrood (EFB) disease, can be followed by infecting to the secondary bacteria, such as Enterococcus faecalis and Brevibacillus laterosporus. The aim of this research was to diagnose EFB disease by tracking the causes of the disease in apiaries all over Iran. From 260 apiaries, honey bee samples were randomly collected. After samples preparation, the genomic DNA was extracted and specific primers were selected for interested bacteria. Using the conventional polymerase chain reaction (PCR) method for E. faecalis and B. laterosporus and nested-PCR method for M. plutonius, the target fragments were amplified. Desired standard bacteria and distilled water were used as positive and negative controls, respectively. Results showed that out of 260 samples from apiaries, 74 and three samples were positive for E. faecalis and B. laterosporus bacteria, respectively. Also, the results of nested-PCR showed that 58 samples were positive, of which only 12 samples were positive in the evaluation of E. faecalis. Results demonstrated that the highest and the least levels of the infection for M. plutonius and E. faecalis were in the south and east of the country, respectively. Results indicated that sometimes due to the excessive growth of secondary bacteria, the main bacteria can be removed from the environment. Also, findings proposed that those provinces with higher number of populations, followed by higher amount of air pollution, had more infected samples than others.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog. 两种严重的情况，一种突然丧失：狗的肠系膜扭转和免疫双丝虫炎。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023716.4197

Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović

{"title":"Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog.","authors":"Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović","doi":"10.30466/vrf.2024.2023716.4197","DOIUrl":"10.30466/vrf.2024.2023716.4197","url":null,"abstract":"Sudden death has been thoroughly well defined and investigated in human medicine and its definition is not as rigorously established in veterinary medicine. In this case a 7-year-old German Shepherd was brought to the Small Animals Clinic of the Faculty of Veterinary Medicine at the University of Belgrade in very poor state. Despite treatment, the dog died 15 min after being admitted to the clinic. Since poisoning was suspected, the owners requested an autopsy. The dog was taken to the Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Belgrade for necropsy. Pathomorphological examinations revealed rupture of the mesentery with mesenteric volvulus. In addition, a large number of adult forms of Dirofilaria immitis were found in the right atrium, ventricle and in truncus pulmonalis. Further examinations of the respiratory system revealed clusters with partially degraded adult parasites in the lobes. The dog died due to mesenteric volvulus which was a rare finding, with an even rarer finding that was lung nodules with adult D. immitis parasites.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"125-128"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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