Veterinary Research Forum最新文献_第2页

Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023716.4197

Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović

{"title":"Two serious conditions, one sudden loss: mesenteric volvulus and dirofilaria immitis in a dog.","authors":"Dajana Davitkov, Sofija Šolaja, Darko Davitkov, Danica Bogunović, Milan Rajković, Vanja Krstić, Anja Ilić Božović","doi":"10.30466/vrf.2024.2023716.4197","DOIUrl":"10.30466/vrf.2024.2023716.4197","url":null,"abstract":"Sudden death has been thoroughly well defined and investigated in human medicine and its definition is not as rigorously established in veterinary medicine. In this case a 7-year-old German Shepherd was brought to the Small Animals Clinic of the Faculty of Veterinary Medicine at the University of Belgrade in very poor state. Despite treatment, the dog died 15 min after being admitted to the clinic. Since poisoning was suspected, the owners requested an autopsy. The dog was taken to the Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Belgrade for necropsy. Pathomorphological examinations revealed rupture of the mesentery with mesenteric volvulus. In addition, a large number of adult forms of Dirofilaria immitis were found in the right atrium, ventricle and in truncus pulmonalis. Further examinations of the respiratory system revealed clusters with partially degraded adult parasites in the lobes. The dog died due to mesenteric volvulus which was a rare finding, with an even rarer finding that was lung nodules with adult D. immitis parasites.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"125-128"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2026240.4232

Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani

{"title":"Epidemiological study for detection of the main and secondary agents of European foulbrood disease in the apiaries of Iran.","authors":"Shirin Dehghan, Masoumeh Bagheri, Mojtaba Moharrami, Hosein Modirrousta, Naheed Mojgani","doi":"10.30466/vrf.2024.2026240.4232","DOIUrl":"https://doi.org/10.30466/vrf.2024.2026240.4232","url":null,"abstract":"Infecting to Melissococcus plutonius, the primary cause of the European foulbrood (EFB) disease, can be followed by infecting to the secondary bacteria, such as Enterococcus faecalis and Brevibacillus laterosporus. The aim of this research was to diagnose EFB disease by tracking the causes of the disease in apiaries all over Iran. From 260 apiaries, honey bee samples were randomly collected. After samples preparation, the genomic DNA was extracted and specific primers were selected for interested bacteria. Using the conventional polymerase chain reaction (PCR) method for E. faecalis and B. laterosporus and nested-PCR method for M. plutonius, the target fragments were amplified. Desired standard bacteria and distilled water were used as positive and negative controls, respectively. Results showed that out of 260 samples from apiaries, 74 and three samples were positive for E. faecalis and B. laterosporus bacteria, respectively. Also, the results of nested-PCR showed that 58 samples were positive, of which only 12 samples were positive in the evaluation of E. faecalis. Results demonstrated that the highest and the least levels of the infection for M. plutonius and E. faecalis were in the south and east of the country, respectively. Results indicated that sometimes due to the excessive growth of secondary bacteria, the main bacteria can be removed from the environment. Also, findings proposed that those provinces with higher number of populations, followed by higher amount of air pollution, had more infected samples than others.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An outbreak of Fusobacterium necrophorum associated foot rot in a herd of four-horned antelope (Tetracerus quadricornis) in India.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2028598.4269

Susitha Rajkumar, Loveleen Maria Vaz, Mathavan Anandhi, Karikalan Mathesh, Shivasharanappa Nayakvadi, Solomon Rajkumar, Shirish Dadarao Narnaware

{"title":"An outbreak of Fusobacterium necrophorum associated foot rot in a herd of four-horned antelope (Tetracerus quadricornis) in India.","authors":"Susitha Rajkumar, Loveleen Maria Vaz, Mathavan Anandhi, Karikalan Mathesh, Shivasharanappa Nayakvadi, Solomon Rajkumar, Shirish Dadarao Narnaware","doi":"10.30466/vrf.2024.2028598.4269","DOIUrl":"https://doi.org/10.30466/vrf.2024.2028598.4269","url":null,"abstract":"An outbreak of contagious foot rot due to Fusobacterium necrophorum infection was reported in a herd of four-horned antelopes (Tetracerus quadricornis) in a wildlife sanctuary at Goa, India. The infected animals showed signs of anorexia, limping and foot lesions, like erosions and sloughing of hooves. Mortality was recorded in two antelopes in which gross lesions, like generalized congestion of skeletal muscles and internal organs, and multi-focal to coalescing abscesses in lung, pleura, and diaphragm were observed. Histopathological evaluation of interdigital skin showed zones of coagulative necrosis, infiltration of mononuclear inflammatory cells, congestion, hemorrhage, and thrombosis in the dermis. Lung showed extensive areas of consolidation with alveolar edema, hyperemic alveolar capillaries, hemorrhage, and infiltration of inflammatory cells. The bacterial culture from heart blood, lung, liver, and kidney tissues revealed isolation of F. necrophorum being also confirmed by polymerase chain reaction (PCR) using amplification of lktA gene of F. necrophorum. In addition, the PCR also confirmed the presence of F. necrophorum DNA in the interdigital skin, lung, and liver tissues. Phylogenetic analysis showed that the F. necrophorum isolate from the present study grouped closely with isolates from Australia, China, Pakistan, and Iraq, as well as an Indian isolate from goat. The affected live animals were treated with oral antibiotics, anti-inflammatory medicines, and vitamin supplements for seven days, and recovered. This study confirmed the first outbreak of contagious foot rot and systemic infection with F. necrophorum in a herd of four-horned antelopes in India.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"51-56"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on syndemic infection of porcine reproductive and respiratory syndrome virus with porcine circovirus 2 in backyard pigs of Mizoram, India. 印度米佐拉姆省散养猪群猪繁殖与呼吸综合征病毒与猪圆环病毒 2 综合感染研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2023688.4187

Amitava Paul, Tridib Kumar Rajkhowa, Kiran Jayappa

{"title":"Studies on syndemic infection of porcine reproductive and respiratory syndrome virus with porcine circovirus 2 in backyard pigs of Mizoram, India.","authors":"Amitava Paul, Tridib Kumar Rajkhowa, Kiran Jayappa","doi":"10.30466/vrf.2024.2023688.4187","DOIUrl":"10.30466/vrf.2024.2023688.4187","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus 2 (PCV2) are among one of the most prevalent and important infectious agents, which cause a severe economic impact on pig farming worldwide. Circulation of these viruses together in the same pig population may lead to the syndemic infection with altered pathogenicity and thereby pathology and clinical manifestation of the diseases. The present study evaluated syndemic infection between PRRSV and PCV2 in the pig population reared in the backyard farms in Mizoram state of India. The syndemicity between PRRSV and PCV2 was confirmed by clinico-pathological studies followed by simultaneous detection of both the viruses in tissue samples by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR), immunohisto-chemistry and SYBR green-based real-time PCR / RT- PCR. The syndemicity resulted to a more severe respiratory disease in affected pigs. Pathological studies on affected pigs revealed a combined picture of hemorrhagic lesions with lymphadenopathy. The SYBR green-based absolute quantification assay estimated the PCV2 load in more quantities than that of PRRSV in all the tissues. The highest PRRSV load was detected in the lungs, while the highest PCV2 load was detected in mesenteric and inguinal lymph nodes. The co-circulation of different pathogenic viruses and their persistent infections in a population is always a matter of great concern to pig producers across the world. The present findings highlighted the similar situation with syndemic infection of PRRSV with PCV2 for the first time in Mizoram, India, that caused severe respiratory disease in affected pigs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"81-88"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2027790.4254

Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh

{"title":"Genotyping and phylogenetic analysis of Mannheimia haemolytica isolates from cattle and buffaloes of West Azerbaijan, Iran.","authors":"Safa Farahmand-Azar, Amir Tukmechi, Abdolghaffar Ownagh","doi":"10.30466/vrf.2024.2027790.4254","DOIUrl":"https://doi.org/10.30466/vrf.2024.2027790.4254","url":null,"abstract":"This study was conducted in West Azerbaijan province, Iran (37°27'18.022\" N, 45°0'0\" E) to investigate the genotyping and phylogenetic characterization of Mannheimia haemolytica in cattle and buffaloes from November 2022 to January 2024. Mannheimia haemolytica is a bacterium known to cause pasteurellosis pneumonia, a respiratory disease in ruminants, such as cattle and sheep. This is one of the main causes of economic losses in the feedlot industry. In addition to the deaths, treatment costs are also significant. The lung and nasal swab samples were collected from 378 cattle and buffaloes. The M. haemolytica was detected in 32 (8.46%) of the samples, with a notably higher isolation rate from lung tissue (56.25%; n = 18) compared to the nasal swabs (43.75%; n = 14). Interestingly, the study also revealed a seasonal pattern, with the highest isolation rates observed during January, February, and March. Multi-locus sequence typing demonstrated that all isolates belonged to sequence type 1 (ST1) within clonal complex 28. This finding is consistent with the global prevalence of ST1 in bovine isolates, indicating widespread distribution. Phylogenetic analysis revealed a strong correlation between ST1 and STs 30 and 54, highlighting the prevalence of ST1 in M. haemolytica among ruminants in West Azerbaijan, Iran. Further research is needed to investigate its potential for causing disease and its transmission pattern.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"11-18"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cage density and meat storage period on some meat quality parameters in brown and white spent hens.

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2025425.4210

Uğur Özentürk, Ayşe Uysal, Ekrem Laçin, Soner Uysal, Sevda Urçar Gelen, Hayrunnisa Özlü

{"title":"The effect of cage density and meat storage period on some meat quality parameters in brown and white spent hens.","authors":"Uğur Özentürk, Ayşe Uysal, Ekrem Laçin, Soner Uysal, Sevda Urçar Gelen, Hayrunnisa Özlü","doi":"10.30466/vrf.2024.2025425.4210","DOIUrl":"10.30466/vrf.2024.2025425.4210","url":null,"abstract":"This study was conducted to determine the effect of different cage densities and meat storage periods on some meat quality parameters in drumstick and breast meat of brown and white spent hens. The study used three different cage densities as low (5 hens per cage), normal (seven hens per cage) and high (10 hens per cage) including 396 hens as 198 Hy-Line Brown (HB) and 198 Isa Tinted (IT). The feeding of chickens was ad libitum (20 - 60 weeks). At the end of the study, a total number of 54 chickens (27 HB and 27 IT) were slaughtered. Some chemical and microbiological analyses were carried out by separating the drumstick and breast area of the slaughtered chickens. According to the results of the research, the redness and yellowness values of the breast area were higher in the ITs. The effect of cage density was significant only for yellowness in the drumstick area. The effects of storage time, lightness and yellowness in the drumstick area and lightness value in the breast area were found to be significant. Bacterial density and thiobarbituric acid reactive substances values of both breast and drumstick regions of spent hens were higher in HBs, while pH values were higher in ITs. The bacterial density in the meat was increased during the storage period. In conclusion, cage density, genotype and storage time affected the microbiological and chemical quality of spent chicken meat. With this result, it can be said that meat quality studies, which mostly focus on broilers, are also important in spent hens.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"71-79"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital. 西班牙一家兽医教学医院从环境和兽医人员中分离出的葡萄球菌的流行率和抗微生物耐药性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2016107.4086

Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez

{"title":"Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital.","authors":"Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez","doi":"10.30466/vrf.2024.2016107.4086","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016107.4086","url":null,"abstract":"Methicillin-resistant Staphylococcus (MRS) bacteria, including methicillin-resistant S. aureus and methicillin-resistant S. pseudintermedius (MRSP), pose a significant threat in veterinary medicine, given their potential for zoonotic transmission and their implications for companion animals and humans' health. This study aimed to assess the prevalence of MRS and anti-microbial resistance patterns at a university clinical hospital in Madrid, Spain. Samples were collected from both the environment and hospital staff at Veterinary Clinical Hospital of Alfonso X el Sabio University. Anti-microbial susceptibility assays, molecular detection of mecA gene and genetic relationships among the identified bacterial strains were performed. The study revealed an MRS prevalence of 1.50% in environmental samples, with MRSP accounting for 0.75% of the cases. Genetically related MRSP strains were found in different hospital areas. Among hospital staff, there was a MRS prevalence of 14.03%, including S. pseudintermedius and S. epidermidis strains. Antibiogram tests revealed multi-drug resistance among MRSP strains. Additionally, methicillin-resistant coagulase-negative staphylococci were isolated, suggesting potential cross-species transmission. This study underscores the presence of MRS in a veterinary clinical hospital, highlighting the significance of infection control through the implementation of protective measures, stringent hygiene practices among personnel and in the environment and responsible use of antibiotics. Further research is necessary to assess MRS incidence in animal patients and explore geographical variations, enhancing our understanding of MRS in veterinary medicine and addressing its challenges.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"325-334"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran. 用分离、血清学和分子方法筛查伊朗东阿塞拜疆省的马勒氏伯克霍尔德氏菌。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2024.2010651.4002

Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi

{"title":"Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran.","authors":"Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi","doi":"10.30466/vrf.2024.2010651.4002","DOIUrl":"10.30466/vrf.2024.2010651.4002","url":null,"abstract":"Glanders caused by Burkholderia mallei is one of the most dangerous zoonotic diseases in solipeds. Clinical diagnosis of this disease in its early stages in horses, is difficult. This study investigated serological and molecular identification of B. mallei in East Azerbaijan province. In the third and fourth quarters of 2020, throughout 2021, and in the first and second quarters of 2022, the complement fixation test (CFT) was performed on 350 horses. The malleination was used to confirm the positive CFT cases. Blood samples were taken for culture and for preparing serums to perform the enzyme-linked immunosorbent assay (ELISA). Deep eye discharge, nostril, cutaneous ulcers and lymph fluid swabs were cultured, and polymerase chain reaction (PCR) was carried out. Eleven horses were CFT-positive. Based on the malleination on the 11 horses, six were affected by glanders, five were not affected (false positive), and one horse was CFT-negative despite exhibiting clinical signs. It was positive by malleination, ELISA and PCR. A total number of seven positive cases of glanders were diagnosed. The B. mallei could not be isolated, but the Burkholderia cepacia complex was isolated in one case. Except for three cases, the results of the CFT, mallein and ELISA tests were consistent. The amount of confidence interval was 95.00%. It is suggested that ELISA could be used as a complement to CFT in screening and, if positive results are observed in one of the tests, the entire herd must be examined more accurately using the mallein and western blot confirmatory tests.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"231-236"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt. 从埃及驼奶中分离出的金黄色葡萄球菌抗菌药耐药性基因的分子特征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2023.2007392.3946

Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey

{"title":"Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt.","authors":"Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey","doi":"10.30466/vrf.2023.2007392.3946","DOIUrl":"10.30466/vrf.2023.2007392.3946","url":null,"abstract":"Staphylococcus aureus is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of S. aureus in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for S. aureus using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of S. aureus isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for S. aureus. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (mecA), β-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDRS aureus as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant S. aureus isolates.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"267-274"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice. 比较某些氨基糖苷类抗生素对小鼠运动行为的影响

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2001972.3891

Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin

{"title":"Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice.","authors":"Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin","doi":"10.30466/vrf.2023.2001972.3891","DOIUrl":"10.30466/vrf.2023.2001972.3891","url":null,"abstract":"Aminoglycoside antibiotics (AGs) can cause neuromuscular blockade and paralysis of skeletal muscles. To compare the paralytic effects of selected AGs on some motor behaviors in mice, 24 male mice were divided into four groups. Each group was given one of AGs (gentamicin, dihydro-streptomycin, apramycin and amikacin) at incremental doses that increased half-logarithmically compared to the therapeutic dose (16.00 mg kg-1). Motor behavioral tests included open field test, inclined plane, horizontal bars, static rods, parallel bars and rotarod. Finally, the data were analyzed using descriptive and analytical statistics. Gentamicin and dihydrostreptomycin at 32.00 times of the therapeutic dose produced complete paralysis of the limbs, respiratory arrest, and even death in some animals. However, apramycin and amikacin did not show significant effects on skeletal muscle and motor behaviors at 32.00 times of the therapeutic dose. After administration of apramycin at 100 times of the therapeutic dose, four out of six mice (66.67%) died from respiratory depression. Amikacin at this dose did not cause animal death, although it caused some changes in motor behaviors with a significant difference in comparison with control values. Gentamicin demonstrated significantly more potent effects on motor behaviors compared to the other AGs. Overall, the order of potency was gentamicin > dihydrostreptomycin > apramycin > amikacin. High doses of AGs could impair the skeletal muscle function and disrupt motor behaviors in mice. Furthermore, the paralytic potency of selected AGs on skeletal muscle was significantly different.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"97-104"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0