Veterinary Research Forum最新文献_第2页

Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. 比较乙酰丙嗪-氯硝柳胺与乙酰丙嗪-氯硝柳胺复方制剂对马的镇静和心血管作用。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2004451.3910

Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei

{"title":"Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses.","authors":"Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2023.2004451.3910","DOIUrl":"10.30466/vrf.2023.2004451.3910","url":null,"abstract":"The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg-1) in combination with acepromazine maleate (0.05 mg kg-1) intravenously (IV). In treatment II (T2), the animals received intra-gastric administration of clonidine (0.002 mg kg-1) followed by acepromazine (0.05 mg kg-1; IV) after 60 min. Head height above the ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 min before and 5, 15, 30, 60, and 90 min after drug administration. In T2, recordings were made 5 min before clonidine, 55 min after clonidine administration, and then 5, 15, 30, 60, and 90 min after acepromazine injection. Analyses of the data showed there were not significant differences regarding HHAG and echocardiographic indices between two treatments. For sedation of healthy horses, it was concluded that intra-gastric administration of clonidine and IV administration of acepromazine showed similar sedative and cardiovascular effects compared to IV acepromazine-xylazine administration.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Brucella spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023. 伊朗工业化奶牛场布鲁氏菌属循环特征：2016 - 2023 年实地研究。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2012972.4028

Saeed Alamian, Karim Amiry, Afshar Etemadi, Maryam Dadar

{"title":"Characterization of Brucella spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023.","authors":"Saeed Alamian, Karim Amiry, Afshar Etemadi, Maryam Dadar","doi":"10.30466/vrf.2024.2012972.4028","DOIUrl":"10.30466/vrf.2024.2012972.4028","url":null,"abstract":"Bovine brucellosis, an infectious disease transmitted by Brucella melitensis and Brucella abortus, presents a significant zoonotic risk for agricultural economics and animal health. The primary objective of this study was to present a comprehensive understanding of the prevalence and features of Brucella strains within the industrial dairy farming sector in Iran. Rose Bengal plate test, standard agglutination test, and indirect enzyme linked immunosorbent assay tests were used to confirm all seropositive animals. A total number of 1,311 bovine samples from seropositive animals including were collected from 224 farms in 21 provinces of different regions of Iran and examined. The discovered Brucella isolates were phenotyped and molecularly characterized. The isolates were all B. abortus or B. melitensis. Bacteria analysis revealed that 70.53% of seropositive farms were tested positive for Brucella strains, predominantly B. melitensis biovar 1 (43.42%) and B. abortus biovar 3 (27.11%). Geographical distribution revealed that B. melitensis biovar 1 was the most common in dairy cow farms (16 provinces), followed by B. abortus biovar 3 (six provinces). Also, the prevalence of B. melitensis biovar 2, B. melitensis biovar 3, B. abortus biovar 1, B. abortus biovar 2 and RB51 vaccine were restricted to certain provinces. AMOS (abortus melitensis ovis suis)-polymerase chain reaction and Bruce-ladder PCR confirmed species identification. These results highlighted the complexity of bovine brucellosis in Iran and illustrated that B. melitensis was spread from small ruminants to cattle. This study provided important epidemiological insights for targeting future brucellosis control programs in the Iranian dairy farms.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generalized megaesophagus associated with Schmidt-like syndrome in a dog. 与施密特样综合征相关的犬全身巨食管症。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2011964.4014

Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei

{"title":"Generalized megaesophagus associated with Schmidt-like syndrome in a dog.","authors":"Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2011964.4014","DOIUrl":"10.30466/vrf.2024.2011964.4014","url":null,"abstract":"A 2-year-old intact male Asian Shepherd dog was referred with a history of chronic regurgitation along with normal appetite and diagnosis of megaesophagus on plain radiography. Clinical examination revealed normothermia, normocardia, normopnea, low body condition score and poor hair coat. The most important laboratory findings include anemia, azotemia, hyperlipidemia, increased thyroid stimulating hormone, decreased thyroxine and hypocortisolemia, as well as a marked increase in acetylcholine receptor antibody concentration. Based on the results, in addition to primary hypothyroidism and primary hypoadrenocorticism, myasthenia gravis was also diagnosed as an underlying cause of megaesophagus. Following nursing care and preferred treatment of each disease, the megaesophagus was resolved in the next visit. This clinical report describes for the first time, to the authors' knowledge, a dog with a rare type of autoimmune polyglandular syndrome (APS) known in human medicine as a Schmidt's syndrome. We want to emphasize the importance of clinicians' awareness regarding the possibility of APS to identify different diseases caused by it in order to achieve successful treatment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Listeria activity of postbiotics of Lactiplantibacillus sakei in beef fillet using aerosolization technique. 利用气溶胶技术在牛肉片中添加清酒乳杆菌后生菌的抗李斯特菌活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2023.2014356.4044

Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani

{"title":"Anti-Listeria activity of postbiotics of Lactiplantibacillus sakei in beef fillet using aerosolization technique.","authors":"Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani","doi":"10.30466/vrf.2023.2014356.4044","DOIUrl":"10.30466/vrf.2023.2014356.4044","url":null,"abstract":"In recent years, the use of probiotics and their metabolites, known as postbiotics as natural preservatives has received increasing attention in the food industry. This study aimed to prepare and characterize postbiotics of Lactiplantibacillus sakei and to investigate its application as an anti-Listeria solution on beef fillets using an aerosolization technique. The functional groups, including organic acids, polysaccharides and other minor metabolites, were identified by Fourier transform infrared (FTIR) in the postbiotics. The 2, 2'-diphenyl-1-picrylhydrazyl radical scavenging activity of the postbiotics was reported as 0.82 mg mL-1. The antimicrobial test using the agar well diffusion method revealed a zone of inhibition of 27.00 ± 1.20 mm. Application of an aerosolized postbiotics solution resulted in a significant reduction in Listeria monocytogenes counts on beef fillets, reaching 3.30 log10 CFU g-1 over a 15-day storage period at 4.00 ± 1.00 ˚C. The results of this study revealed that the postbiotics of L. sakei was an effective antimicrobial additive for controlling foodborne pathogens in beef fillets and aerosolization is a promising method for developing an antimicrobial coating on meat to enhance meat safety.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of the beta-lactamase Cefotaxime gene in Escherichia coli from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island. 从龙目岛重复繁殖的巴厘岛牛生殖道中分子检测大肠杆菌中的β-内酰胺酶头孢他啶基因。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2014818.4056

Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati

{"title":"Molecular detection of the beta-lactamase Cefotaxime gene in Escherichia coli from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island.","authors":"Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati","doi":"10.30466/vrf.2024.2014818.4056","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014818.4056","url":null,"abstract":"This study aims to detect Escherichia coli which encodes beta-lactamase Cefotaxime (blaCTX-M) gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of E. coli was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of E. coli which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the blaCTX-M gene of E. coli and sequences of the blaCTX-M gene were phylogenetically analyzed. The research results obtained three E. coli isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of E. coli was resistant to penicillin G and oxytetracycline. 66.66% of E. coli was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of E. coli was resistant to tetracycline. Escherichia coli isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of E. coli detection using PCR showed that three E. coli isolates encoded the blaCTX-M gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that E. coli from the reproductive tract of Bali cattle was related to E. coli that encoded blaCTX-M-14 isolated from humans.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system. 姜黄素对福尔马林诱导的雄性大鼠肌肉疼痛的影响：局部环氧化酶系统的作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2015987.4069

Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad

{"title":"Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system.","authors":"Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2024.2015987.4069","DOIUrl":"https://doi.org/10.30466/vrf.2024.2015987.4069","url":null,"abstract":"Investigating the mechanisms responsible for pain processing of natural and synthetic chemical compounds is necessary to optimize pain management. Curcumin (Cur), the active ingredient of turmeric, exhibits potent analgesic and anti-inflammatory properties by employing multiple mechanisms at the local peripheral, spinal and supra-spinal levels. This study was aimed to investigate the effect of oral administration of Cur on muscle pain induced by intramuscular (IM) injection of formalin. To explore the possible local mechanisms, a cyclooxygenase (COX) inhibitor, diclofenac (Dic) and a COX product, prostaglandin E2 (PGE2), were applied. The IM injection of formalin (25.00 µL, 2.50%) into the gastrocnemius muscle induced two distinct phases of hind leg flinching. A short-lasting (10 min) hind leg lifting was observed following IM injection of PGE2 (2 µg kg-1, 25.00 µL). Oral administration of Cur (25.00 and 100 mg kg-1) and IM injection of 40.00 µg kg-1 Dic attenuated formalin and PGE2 induced nociceptive behaviors. Contra-lateral IM injection of Dic did not change muscle pain induced by ipsilateral IM injection of formalin and PGE2. The second phase of formalin induced flinching as well as PGE2 evoked lifting were more suppressed when 40.00 µg kg-1 Dic and 100 mg kg-1 Cur were used together. Locomotor activity was not changed by the above-mentioned treatments. It was concluded that the reducing effect of muscle pain of Cur might be related to the local inhibition of COX.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of virulence and extended spectrum β-lactamase producing genes of Klebsiella pneumoniae isolated from bovine milk. 从牛乳中分离的肺炎克雷伯氏菌产生毒力和广谱β-内酰胺酶基因的遗传特征。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2006974.3947

Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki

{"title":"Genetic characterization of virulence and extended spectrum β-lactamase producing genes of Klebsiella pneumoniae isolated from bovine milk.","authors":"Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki","doi":"10.30466/vrf.2023.2006974.3947","DOIUrl":"10.30466/vrf.2023.2006974.3947","url":null,"abstract":"Mastitis associated Klebsiella pneumoniae species were isolated from bovine milk to characterize virulence genes (wabG and kfuBC) and extended spectrum β-lactamase (ESBL) genes (blaCTX-M-1, blaCTX-M-2, blaCTX-M-9, blaTEM, blaSHV and blaOXA). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 K. pneumoniae isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of wabG (25/27; 92.59%) was higher than kfuBC (5/27; 18.51%). Beta-lactamase genes viz., blaSHV, blaTEM and blaCTX-M-1 were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for blaCTX-M-9 and blaOXA-1 genes. Outcome of the study provided an insight into virulence genes and ESBL producing K. pneumoniae isolated from bovine milk samples in India.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection and phylogenetic analysis of Borrelia spp. from sheep and goats blood samples in West Azerbaijan province, Iran. 伊朗西阿塞拜疆省绵羊和山羊血液样本中 Borrelia spp.的分子检测和系统发育分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2000526.3855

Ahmad Enferadi, Abdolghaffar Ownagh, Mousa Tavassoli

{"title":"Molecular detection and phylogenetic analysis of Borrelia spp. from sheep and goats blood samples in West Azerbaijan province, Iran.","authors":"Ahmad Enferadi, Abdolghaffar Ownagh, Mousa Tavassoli","doi":"10.30466/vrf.2023.2000526.3855","DOIUrl":"10.30466/vrf.2023.2000526.3855","url":null,"abstract":"Borrelia species are spirochetes transmitted by ticks that are important in human and animals. In most countries, there is still no molecular epidemiology of borreliosis in ruminants. This study was aimed to evaluate the existence of Borrelia spp. DNA in the blood samples of small ruminants using polymerase chain reaction (PCR) method in West Azerbaijan Province, Iran. To detect Borrelia spp. DNA, about 1,018 ruminants (456 goats and 562 sheep) blood samples were examined from different bioclimatic regions in West Azerbaijan province, Iran. The DNA extracting and PCR were conducted. In sheep, the following prevalence rates were respectively obtained for the 16S rRNA, 5S - 23S rRNA and ospA genes: 3.55% (20/562), 2.13% (12/562) and 0.88% (5/562). And so, the prevalence rates of the genes in goats were 0.87% (4/456) for 5S - 23S rRNA gene, 1.75% (8/456) for 16S rRNA gene and 0.65% (3/456) for ospA gene. The prevalence of Borrelia spp. was significantly different in small ruminants based on the farms and localities. The sheep and goats in humid areas (north of West Azerbaijan) were infected statistically more than those in sub-humid areas (south of West Azerbaijan). It is demonstrated that host species like sheep and goats may have a key role in natural Lyme disease cycles and other borreliosis diseases in Iran.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal alteration in ovarian follicle fate with hormonal and hemodynamic perspectives during receptive phase of estrous cycle in endometritic cattle. 子宫内膜炎牛发情周期受孕期卵泡命运的时间变化与激素和血液动力学的关系

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2013227.4033

Akshay Sharma, Pravesh Kumar, Pankaj Sood, Vijender Negi, Pururava Sharma

{"title":"Temporal alteration in ovarian follicle fate with hormonal and hemodynamic perspectives during receptive phase of estrous cycle in endometritic cattle.","authors":"Akshay Sharma, Pravesh Kumar, Pankaj Sood, Vijender Negi, Pururava Sharma","doi":"10.30466/vrf.2023.2013227.4033","DOIUrl":"10.30466/vrf.2023.2013227.4033","url":null,"abstract":"The present research was carried out to assess the serum progesterone (P4) concentration and uterine hemodynamics at estrus till ovulation in cyclic cows (N = 130) with healthy or diseased uterus. At estrus, 85 cows were diagnosed with clinical endometritis (CE; n = 44) and sub-clinical endometritis (SCE; n = 41); whereas, 45 cows being served as control namely no endometritis (NE; n = 45) were included in the study. Serum progesterone estimation at 12 - 14 and 40 hr after the onset of estrus and Doppler sonography of both middle uterine arteries were done to envisage the uterine hemodynamics and ovulation. The serum progesterone concentration was significantly higher at 12 - 14 hr after onset of estrus in CE and SCE cows. At 12 - 14 hr after onset of estrus, a cut-off value of ≥ 0.48 ng mL-1 P4 was obtained, above which 22.72% CE, 26.82% SCE and only 8.88% NE cows failed to ovulate within 36 - 40 hr of estrus onset. Among the Doppler indices, pulsatility and resistance indices were significantly higher; whereas, volume and velocity indices were significantly lower in NE cows. In cows diagnosed with CE and SCE, a higher supra-basal P4 concentration, and velocity and volume of blood flow to uterus at estrus negatively affected the duration to ovulation.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of an insulated isothermal polymerase chain reaction assay for the rapid detection of Mycoplasma synoviae. 开发和验证用于快速检测滑膜支原体的绝缘等温聚合酶链反应测定法。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2022.554037.3474

Lucai Wang, Lijia Liu, Huanrong Zhang

{"title":"Development and validation of an insulated isothermal polymerase chain reaction assay for the rapid detection of Mycoplasma synoviae.","authors":"Lucai Wang, Lijia Liu, Huanrong Zhang","doi":"10.30466/vrf.2022.554037.3474","DOIUrl":"10.30466/vrf.2022.554037.3474","url":null,"abstract":"Mycoplasma synoviae, which causes the disease known as chicken synovitis, causes serious immunosuppression. We developed a rapid insulated isothermal polymerase chain reaction (iiPCR) assay for on-site detection of M. synoviae using a primer and probe set targeting the variable lipoprotein and haemagglutinin (vlhA) gene. In addition, the specificity, sensitivity, repeatability, and clinical detection of this method were evaluated. Our iiPCR assay detected M. synoviae clinical isolates and samples successfully and produced negative results on Mycoplasma galliscepticum, avian viral arthritis, Escherichia coli, Salmonella, Staphylococcus aureus and Corynebacterium, indicating that the PCR reactions were specific. Additionally, our iiPCR assay detected the prepared positive standard plasmid diluted 10 times (1.00 × 10-1 - 1.00 × 10-10) as a template. The undiluted positive plasmid was positive and double distilled water was negative indicating that the PCR reactions were sensitive, respectively. Finally, the vlhA positive standard plasmid with dilution multiple of 1.00 × 10-4 - 1.00 × 10-6 was repeatedly detected three times to evaluate the repeatability of the iiPCR method established in this experiment showing that the iiPCR of M. synoviae is repeatable. The established iiPCR was also used to detect 50 chicken joint enlargement samples. The thermostatic detection PCR established in this experiment was comparable to a reference real-time PCR (qPCR).","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0