Veterinary Research Forum最新文献_第2页

In-vitro evaluation and efficacy of bacteriophage isolated from commercial poultry farms against Salmonella typhimurium. 商业家禽养殖场分离的噬菌体抗鼠伤寒沙门菌的体外评价和效果。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2030436.4305

Amina Zaib, Ali Ahmad Sheikh, Faisal Ayub, Muhammad Arshad Durrani, Mobashra Mustafa, Afifa Shahzad, Urooj Ejaz, Muhammad Zubair Latif, Chamman Zahra, Izza Izza, Fareeha Zaib, Husnain Ahmad, Muhammad Jawad Hafeez, Muhammad Mujahid, Hafiz Muhammad Moavia Atique

{"title":"In-vitro evaluation and efficacy of bacteriophage isolated from commercial poultry farms against Salmonella typhimurium.","authors":"Amina Zaib, Ali Ahmad Sheikh, Faisal Ayub, Muhammad Arshad Durrani, Mobashra Mustafa, Afifa Shahzad, Urooj Ejaz, Muhammad Zubair Latif, Chamman Zahra, Izza Izza, Fareeha Zaib, Husnain Ahmad, Muhammad Jawad Hafeez, Muhammad Mujahid, Hafiz Muhammad Moavia Atique","doi":"10.30466/vrf.2024.2030436.4305","DOIUrl":"10.30466/vrf.2024.2030436.4305","url":null,"abstract":"Avian Salmonellosis impacts the economy and public health, with chicken products being a major cause of gastroenteritis. Hygiene, immunization and medicines are all used as control techniques. Bacteriophages provide a safe, targeted alternative. In the present study in vitro evaluation of bacteriophages were done against Salmonella typhimurium. Lytic effect of bacteriophages isolated from poultry sludge was checked on culture of S. typhimurium. Stability study was checked at range of temperature and pH. The phages were stable at temperature (30.00 - 50.00 ˚C) and pH (5.00 - 9.00) where best activity was seen at 37.00 ˚C and pH 7.00. In vitro lytic activity was done at (optical density 600 nm) after exposure to bacterial host at different intervals. Multiplicity of Infection of 1.00 was used to check lytic activity of phages which indicated phages were potent enough to infect bacterial cells within their growth cycle. The percentage of unadsorbed phages was determined by bar chart analysis. The genome of three phages was treated with DNase I where they all were sensitive. Later the nucleic acid of phages was digested by restriction endonucleases (EcoRI and HindIII) both of the enzymes produced various restriction sites with different band. The present study proved that the application of bacteriophages in vitro into bacterial system i.e., S. typhimurium was an attractive method in diminishing infection in commercial poultry thus providing exceptional results that could be used on a large scale.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"267-275"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody. 基于免疫球蛋白Y抗体的口蹄疫病毒A血清型特异性抗体固相竞争酶联免疫吸附检测方法的建立。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2025842.4226

Zahra Ivani, Behzad Hemati, Naser Harzandi, Seyed Mahmoud Azimi, Mohammad Mehdi Ranjbar

{"title":"Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody.","authors":"Zahra Ivani, Behzad Hemati, Naser Harzandi, Seyed Mahmoud Azimi, Mohammad Mehdi Ranjbar","doi":"10.30466/vrf.2024.2025842.4226","DOIUrl":"10.30466/vrf.2024.2025842.4226","url":null,"abstract":"The aim of this study was to using purified chicken antibody (IgY) for developing solid phase competitive (SPC) enzyme-linked immunosorbent assay (ELISA) to detect the foot-and-mouth disease virus (FMDV) A serotype. After immunization of chickens, polyclonal immunoglobulin (IgY) antibodies were extracted and purified from egg yolk and yield was about 5.00 mg mL-1 of yolk as well as near 0.40 mg mL-1 of specific IgY antibody against FMDV serotype A. Also, optimized sucrose density gradient method produced 228 µg mL-1 whole virus which is much higher than that of the conventional method of sucrose density gradient method. The optimum concentration of purified capture IgY and bind type A antigen were 0.50 µg and 0.10 µg per well, respectively. The OD values < 0.70 were considered positive, and values ≥ 0.70 were negative for in-house kit base on standard controls. Statistical analysis base on 80 serum samples showed the 96.66% sensitivity, 100% specificity, 100% positive predictive value, 90.90% negative predictive value, 97.50% accuracy, and 98.33% reliability for serum samples for two commercial and in-house kits. The SPCE developed based on IgY antibody is a suitable alternative for the detection of antibodies after vaccination against type A FMDV with high sensitivity and specificity. The present research demonstrated the possibility of commercial development of the SPCE kit using IgY antibodies for the detection of FMDV antibodies in serum samples with adequate sensitivity and accuracy.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"285-292"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing feed efficiency and egg production in Hy-Line W-80 and Iraqi indigenous chickens. 海兰W-80和伊拉克土鸡饲料效率和产蛋率的比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 DOI: 10.30466/vrf.2024.2030349.4297

Dhafer Abdul-Malek Ali, Nihad Abdul-Lateef Ali, Mohammed Baqur Sahib Al-Shuhaib

{"title":"Comparing feed efficiency and egg production in Hy-Line W-80 and Iraqi indigenous chickens.","authors":"Dhafer Abdul-Malek Ali, Nihad Abdul-Lateef Ali, Mohammed Baqur Sahib Al-Shuhaib","doi":"10.30466/vrf.2024.2030349.4297","DOIUrl":"10.30466/vrf.2024.2030349.4297","url":null,"abstract":"The Iraqi indigenous chicken (IIC) is a native breed known for its remarkable ability to thrive in harsh environments. However, the primary challenge with this breed is its lower productivity levels especially when compared to commercial strains. This study evaluated the performance of 120 layers of IIC in comparison with an equal number of Hy-Line W-80 layers across multiple traits including feed efficiency (FE), feed conversion ratio (FCR), daily feed intake (DFI), egg mass (EM) and total egg number (EN) production. Over a period spanning four consecutive weeks intervals significant differences were observed between the two lines across all measured parameters. Hy-Line W-80 consistently exhibited superior FE, with values ranging from 0.49 to 0.54 compared to IIC values that ranged from 0.23 to 0.26. Similarly, Hy-Line W-80 demonstrated lower FCR values ranging from 1.85 to 2.03 contrasting with IIC (3.77 to 4.37). The DFI was notably higher in Hy-Line W-80 (ranging from 104.30 to 106.00 g per day) compared to IIC (73.75 to 90.20 g per day). Furthermore, Hy-Line W-80 consistently outperformed IIC in EM production with values ranging from 52.33 to 56.67 compared to IIC values (19.83 to 21.47). Another superiority of Hy-Line W-80 was observed by showing higher EN over IIC layers in all sixteen weeks of investigation. In conclusion, Hy-Line W-80 consistently outperformed IIC in various parameters such as FE, FCR, DFI, EM and EN production. Accordingly, these data could be exploited in selective breeding and genetic improvement strategies to enhance the productivity of IIC.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"195-201"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture. 聚β-羟基丁酸对鲤鱼鱼种的促生长潜力及免疫刺激作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2024674.4200

Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz

{"title":"Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture.","authors":"Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz","doi":"10.30466/vrf.2024.2024674.4200","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024674.4200","url":null,"abstract":"The natural polymer poly-β-hydroxybutyrate (PHB) is converted to β-hydroxybutyric acid, which is similar to short-chain fatty acids, via microbial fermentation and host enzyme breakdown. This study investigated the impact of different PHB concentrations (Control, 1.00, 3.00, and 5.00% substitution) on growth performances and fish welfare in common carp fingerlings. After a 60-day trial, fish fed on diet containing 1.00% PHB exhibited significantly higher weight gain and improved feed conversion efficiency compared to the control group. Furthermore, analysis of enzymatic activity showed elevated levels of total protease and amylase in PHB-fed treatments compared to the control. Red blood cell counts, hemoglobin, and hematocrit levels remained unaffected and a significant increase in white blood cell count was observed in fish fed on diets containing 1.00 and 3.00% PHB compared to the control group. Furthermore, fish fed on diets containing 1.00 and 3.00% PHB demonstrated significantly higher total protein levels and lower glucose concentrations as well as reduced hepatic enzyme activities (aspartate aminotransferase and alanine aminotransferase) compared to both the control and 5.00% PHB groups. Assessment of antioxidant and immune parameters revealed significantly increased complement hemolytic activity and immunoglobulin M levels coupled with decreased malondialdehyde concentrations in the plasma of PHB-fed fish compared to the control group. In conclusion, dietary supplementation with PHB, especially at the 1.00% level, enhanced growth performance and improved nutritional and health indicators in the fingerlings. These findings suggested that PHB had the potential to be a valuable dietary additive for this species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"43-50"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and application of dual isothermal amplification of Pasteurella multocida and Streptococcus suis in pigs. 猪多杀性巴氏杆菌和猪链球菌双等温扩增方法的建立及应用。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2035817.4371

Shuang Li, Jingjing Li, Kexin Wang, Qianlei Zhu, Yafei Chang, Lei Wang, Zhanwei Teng, Xiaobing Wei, Meinan Chang, Mingcheng Liu, Oksana Kasjanenko, Sergii Kasianenko, Jianhe Hu, Huihui Zhang, Xiaojing Xia

{"title":"Establishment and application of dual isothermal amplification of Pasteurella multocida and Streptococcus suis in pigs.","authors":"Shuang Li, Jingjing Li, Kexin Wang, Qianlei Zhu, Yafei Chang, Lei Wang, Zhanwei Teng, Xiaobing Wei, Meinan Chang, Mingcheng Liu, Oksana Kasjanenko, Sergii Kasianenko, Jianhe Hu, Huihui Zhang, Xiaojing Xia","doi":"10.30466/vrf.2024.2035817.4371","DOIUrl":"10.30466/vrf.2024.2035817.4371","url":null,"abstract":"Porcine respiratory disease complex is a clinically lethal condition and is the leading cause of mortality in weaned piglets as well as growing and fattening pigs. Pasteurella multocida (Pm) and Streptococcus suis (SS) are common respiratory pathogens in porcine respiratory disease complex. This study combined the recombinase polymerase amplification (RPA) technique with the lateral flow dipstick (LFD) technique. The dual Basic-RPA detection method for Pm and SS and the dual RPA-LFD rapid visualization detection method for Pm and SS were constructed, respectively. The detection limit of RPA-LFD was 10-6 ng μL-1, which was higher than the detection limit of RPA at 10-5 ng μL-1 and much higher than the detection limit of polymerase chain reaction at 10-4 ng μL-1. There was no cross-reactivity with other pathogens which indicated that the method had good specificity and high sensitivity. The detection rate of RPA-LFD was much higher than that of conventional Polymerase chain reaction in 60 clinical samples collected in 2023 with suspected Pm and SS. This method could avoid the complicated temperature cycling instruments and does not require professional laboratory skills, which makes it suitable for on-site detection.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"365-374"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The study of spinopelvic parameters measurement by computed tomography in Scottish cats. 用计算机断层扫描测量苏格兰猫脊柱骨盆参数的研究。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2024.2036500.4374

Dorna Ghassemi, Amirali Raissi, Varia Tohidi

{"title":"The study of spinopelvic parameters measurement by computed tomography in Scottish cats.","authors":"Dorna Ghassemi, Amirali Raissi, Varia Tohidi","doi":"10.30466/vrf.2024.2036500.4374","DOIUrl":"10.30466/vrf.2024.2036500.4374","url":null,"abstract":"Understanding spinopelvic parameters is crucial for diagnosing skeletal and muscular disorders. This study was the first to measure spinopelvic parameters, specifically pelvic incidence (PI), pelvic tilt (PT) and sacral slope (SS) in Scottish cats using computed tomography (CT). the research analyzed twelve Scottish cats (4.00 - 6.00 kg, 2 - 3 years old). The mean values obtained were 48.80° for PI, 26.00° for PT and 22.80° for SS. The PI = PT + SS equation was valid in healthy cats but failed in cases with spondylosis and pelvic fractures indicating its potential diagnostic utility. Pearson correlation analysis showed no significant relationship between PI and PT or PI and SS, however, a significant negative correlation between PT and SS was observed. This suggested that as PT increased, SS decreased. The study established baseline values for PI, PT, and SS in Scottish cats and highlighted the potential for using these parameters in feline diagnostics. The failure of the PI = PT + SS equation in pathological cases underscored its role in identifying specific conditions such as spondylosis and pelvic fractures. This foundational data was crucial for assessing spinopelvic alignment and diagnosing spinal disorders in cats. The findings suggested that deviations from baseline values could aid in diagnosing musculoskeletal disorders, optimizing treatment and preventing complications. Further research is needed to explore how variations in these parameters relate to different spinal conditions.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"475-480"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis. 在类风湿性关节炎实验模型中将胡椒碱与泼尼松龙结合使用的益处

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2031860.4316

Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid

{"title":"Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis.","authors":"Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid","doi":"10.30466/vrf.2024.2031860.4316","DOIUrl":"10.30466/vrf.2024.2031860.4316","url":null,"abstract":"This study evaluated the impact of combining piperine and prednisolone on clinical symptoms and immune responses in Wistar rats with rheumatoid arthritis (RA) induced by Freund's complete adjuvant due to piperine known anti-inflammatory and immunomodulatory properties. The RA rats were randomly divided into five groups (n = 10): The RA rats were treated with phosphate-buffered saline, RA rats treated with piperine (100 mg kg-1 orally), RA rats treated with prednisolone (10.00 mg kg-1 orally), and RA rats treated with a combination of piperine and prednisolone (half doses of each orally). Treatment started on day five post-induction when all rats had a clinical score of ≥ 1. Disease symptoms were monitored every other day until day 23 post-induction. Combining the two medications at half doses led to a more significant reduction in disease severity, weight improvement, and histopathological changes compared to using each drug alone at the full doses. The combined treatment group exhibited the most favorable response in C-reactive protein, myeloperoxidase, and nitric oxide biochemical tests compared to the other treatment groups. The combined treatment group showed decreased expression of T-bet and RORɣt genes. However, there was no statistically significant difference in the expression of Foxp3 and GATA3 genes compared to the group receiving prednisolone alone. Overall, combining piperine with prednisolone may prove to be a beneficial approach for managing RA.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"117-124"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the reference values for arterial blood gas, electrolytes and acid-base in healthy male sheep of Iranian Shall breed. 伊朗Shall种健康雄性绵羊动脉血气、电解质和酸碱参考值的测定。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2028629.4270

Sirous Sadeghian Chaleshtori, Mohammad Reza Mokhber Dezfouli, Abolfazl Najd Ghahremani, Neda Sabetzadeh

{"title":"Determining the reference values for arterial blood gas, electrolytes and acid-base in healthy male sheep of Iranian Shall breed.","authors":"Sirous Sadeghian Chaleshtori, Mohammad Reza Mokhber Dezfouli, Abolfazl Najd Ghahremani, Neda Sabetzadeh","doi":"10.30466/vrf.2024.2028629.4270","DOIUrl":"10.30466/vrf.2024.2028629.4270","url":null,"abstract":"The goal of this research was to determine the normal range of arterial blood gas, electrolytes and acid-base status in the healthy male sheep of Iranian Shall breed. For this purpose, the blood sample was collected from the ear artery of 15 Iranian Shall sheep whose health was confirmed by clinical examinations, hematological tests including complete blood count and air-dried blood smear and cardiac evaluations including electrocardiography and echocardiography. Then, the values of pH, partial dioxide carbon pressure, partial oxygen pressure, oxygen saturation, base excess, bicarbonate as well as the concentration of electrolytes and anion gap were measured using a blood gas analyzer. The obtained data were subjected to statistical analyses by SPSS software and the tests of Kolmogorov-Smirnov and Pearson and descriptive statistics of the results were expressed as mean ± SE, range (minimum and maximum) and confidence interval 95.00%. The results showed the mean value of arterial blood gas including oxygen saturation, partial oxygen pressure, and partial dioxide carbon pressure were 95.69%, 61.58, and 26.28 mmHg, respectively, and the others arterial blood parameters including pH, base excess, and bicarbonate were 7.54, 1.61, and 22.84 mmol L-1, respectively. Also, the mean values of arterial blood electrolytes including Na+, K+, and Cl-, were 143.25, 4.22, and 109.46 mmol L-1, respectively, and the mean anion gap was 12.64 mmol L-1. The present study determined the range of normal amounts of arterial blood gas, electrolytes and acid-base status in the healthy male sheep of Iranian Shall breed which could be used as a reference for further research on sheep diseases and to distinguish healthy status from disease.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"175-179"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conventional and molecular identification of Iranian Clostridia species associated with animal infection. 与动物感染相关的伊朗梭状芽孢杆菌的常规和分子鉴定。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2035437.4361

Lida Abdolmohammadi Khiav, Anahita Emadi, Maryam Dadar

{"title":"Conventional and molecular identification of Iranian Clostridia species associated with animal infection.","authors":"Lida Abdolmohammadi Khiav, Anahita Emadi, Maryam Dadar","doi":"10.30466/vrf.2024.2035437.4361","DOIUrl":"10.30466/vrf.2024.2035437.4361","url":null,"abstract":"Clostridial disease causes severe economic losses in livestock by rapidly killing ruminants. Therefore, implementing effective control approaches to prevent this fatal disease is of high importance. The causative agent of this disease is Clostridium spp. Accurate identification of this microorganism is crucial for effectively managing clostridial diseases in farm. There are conventional methods for detecting the disease including microbiological and biochemical tests and many of these tests are time-consuming and exhibit low sensitivity. Therefore, this study aimed to use conventional and molecular approaches to identify Iranian isolates associated with animal infections. To achieve this, 61 samples were collected from 1984 to 2024, cultured on liver media and subsequently subjected to microbiological and biochemical tests. For molecular identification, the DNA of isolates were extracted and confirmed by polymerase chain reaction (PCR) using specific primers. The results of the conventional analysis revealed that all Iranian isolates were identified as Clostridium perfringens and its type was determined using PCR assay. According to our findings, C. perfringens type A was the most prevalent strain in Iran which predominantly found in ostriches and bird samples followed by type D. This study underscored the presence of C. perfringens types across variety hosts and geographic locations in Iran. In conclusion, the combining conventional methods with PCR helped reliably detect Clostridium spp. This information holds the potential to significantly contribute to the development of preventive strategies against clostridial diseases in Iran.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"345-352"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent multi-systemic eosinophilic epitheliotropic disease and choledocholithiasis in a 15-year-old mixed-breed mare. 15岁混血母马并发多系统嗜酸性嗜上皮性疾病和胆总管结石。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2034996.4356

Zahra Baradaran-Seyed, Mohammad Eslampanah

{"title":"Concurrent multi-systemic eosinophilic epitheliotropic disease and choledocholithiasis in a 15-year-old mixed-breed mare.","authors":"Zahra Baradaran-Seyed, Mohammad Eslampanah","doi":"10.30466/vrf.2024.2034996.4356","DOIUrl":"10.30466/vrf.2024.2034996.4356","url":null,"abstract":"A mixed-breed 15-year-old mare was euthanized for recurrent colic, severe weight loss, pastern ulcerative dermatitis, and coronitis without response to non-surgical medical procedures. The liver was congested with firm consistency at necropsy, and there were multi-focal white spots on Glisson's capsule. Hemorrhage, ulcer, severe folding, and hypertrophy were seen in the duodenum and jejunum. Histopathological examination showed marked infiltration of eosinophils concomitant with fewer leukocytes, macrophages, and plasma cells, primarily in the liver and intestines. The presence of eight calcium bilirubinate stones, 2.00 - 5.00 cm in diameter, in the common bile duct was characteristic of choledocholithiasis. Concurrent multi-systemic eosinophilic epitheliotropic disease (MEED) and choledocholithiasis were confirmed after ruling out other potential causes of eosinophilic infiltration, including parasitic infection and allergy. The cause of MEED and choledocholithiasis was unclear because of incomplete case history and delayed referral, with a potential infection playing a role and having synergistic effects between the two conditions. When eosinophilic-associated inflammation involves several organs, MEED should be on the differential diagnostic list since infections and allergens are also among the risk factors for this disorder.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"305-308"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0