Veterinary Research Forum最新文献_第10页

Effects of adenosine N1-Oxide and pioglitazone on inflammatory and antioxidant state in sepsis caused by experimental cecal puncture in rat. 腺苷n1 -氧化物和吡格列酮对实验性盲肠穿刺脓毒症大鼠炎症和抗氧化状态的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.562229.3625

Yaser Jafari-Khataylou, Siamak Kazemi-Darabadi, Somayeh Ahmadi Afshar

{"title":"Effects of adenosine N1-Oxide and pioglitazone on inflammatory and antioxidant state in sepsis caused by experimental cecal puncture in rat.","authors":"Yaser Jafari-Khataylou, Siamak Kazemi-Darabadi, Somayeh Ahmadi Afshar","doi":"10.30466/vrf.2022.562229.3625","DOIUrl":"https://doi.org/10.30466/vrf.2022.562229.3625","url":null,"abstract":"Sepsis is an acute condition caused by the systemic inflammatory response syndrome to an infection. There are very few drugs that could improve the severe conditions in patients with sepsis. Hence, it is important to consider different treatment options. In this survey, we studied the effect of adenosine N1-oxide (ANO) and pioglitazone on rats with cecal ligation and perforation (CLP). They were randomly divided to four groups (n = 10) including Group A: as control group receiving normal saline, Group B: the rats with CLP as surgical control group, Group C: the rats receiving ANO, and Group D: the rats receiving pioglitazone. Interleukin (IL) -6, IL-1β, tumor necrosis factor alpha (TNF-α), nitric-oxide (NO) in serum blood and superoxide dismutase (SOD), catalase (CAT) malondialdehyde, (MDA) and myeloperoxidase (MPO) in liver and spleen homogenates were measured. The amount of antioxidant enzymes in the spleen and liver, and finally cell viability and rats' survival were investigated. The measurement of blood serum nitric-oxide and survival of all groups of rats were also performed. The results indicated that both drugs could cause a decrease in IL-1β, IL-6, TNF-α and NO in rat blood serum and MDA and MPO in the liver and spleen homogenates, however, a significant increase in SOD and CAT in the liver and spleen homogenates in rats that received ANO and pioglitazone was observed compared to rats with CLP group. Cell viability and rats' survival were significantly improved in rats that received ANO and pioglitazone compared to rats with CLP group. Adenosine N1-oxide showed stronger and more effective effects.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 7","pages":"381-387"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/60/vrf-14-381.PMC10410109.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational design of a chimeric toxin against Claudin-4-expressing cancer cells: molecular modeling, docking and molecular dynamics simulation analysis. 针对表达claudin -4的癌细胞嵌合毒素的计算设计:分子建模、对接和分子动力学模拟分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.548415.3378

Sepehr Safaei, Mehdi Imani

{"title":"Computational design of a chimeric toxin against Claudin-4-expressing cancer cells: molecular modeling, docking and molecular dynamics simulation analysis.","authors":"Sepehr Safaei, Mehdi Imani","doi":"10.30466/vrf.2022.548415.3378","DOIUrl":"https://doi.org/10.30466/vrf.2022.548415.3378","url":null,"abstract":"Cancer is one of the main reasons of mortality all over the world. Over the time, the major ways for cancer-therapy were based on radiotherapy, chemotherapy and surgery. These methods are not specific enough for that purpose, therefore, new ideas for design of new drugs with higher specificity are considered. Chimeric protein toxins are hybrid proteins consisting of a targeting portion and a toxic one which specifically bind and kill the target cancer cells. The main purpose of this study was designing a recombinant chimeric toxin with biding capability to one of the most key receptors namely claudin-4 which is over-expressed in almost all cancer cells. To design it, we utilized the last 30 C-terminal amino acids of Clostridium perfringens enterotoxin (CPE) as a binding module for claudin-4 and the toxic module which is the A-domain of Shiga toxin from Shigella dysenteriae. Using molecular modeling and docking methods, appropriate binding affinity of the recombinant chimeric toxin to its specific receptor was demonstrated. In the next step, the stability of this interaction was investigated by molecular dynamics simulation. Although partial instability was detected at some time points, however, sufficient stable situation of hydrogens bonds and high binding affinity between the chimeric toxin and receptor were observed in the in silico studies which in turn suggested that this complex could be formed successfully.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 5","pages":"259-265"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/1e/vrf-14-259.PMC10278906.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10067558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echocardiographic assessment of right ventricular blood flow and tricuspid annular plane systolic excursion in apparently healthy domestic short-haired cats. 超声心动图评价健康家养短毛猫右心室血流和三尖瓣环面收缩偏移。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.548794.3387

Zahra Darabizadeh, Keivan Keramati, Daruish Shirani, Mohammad Molazem, Saeed Heydaryan, Yasamin Vali

{"title":"Echocardiographic assessment of right ventricular blood flow and tricuspid annular plane systolic excursion in apparently healthy domestic short-haired cats.","authors":"Zahra Darabizadeh, Keivan Keramati, Daruish Shirani, Mohammad Molazem, Saeed Heydaryan, Yasamin Vali","doi":"10.30466/vrf.2022.548794.3387","DOIUrl":"https://doi.org/10.30466/vrf.2022.548794.3387","url":null,"abstract":"Right ventricular parameters change in many cardiovascular diseases; so, the presence of normal right ventricular parameters is necessary to diagnose these diseases. Ten clinically healthy adult domestic short-haired cats including six males and four females in the range of 2.70 to 4.80 kg were studied using echocardiography without sedation. The speed and pressure of blood flow through the tricuspid and pulmonary valves, speed of the movement of tricuspid valve and tricuspid annular plane systolic excursion (TAPSE) were recorded using conventional pulsed-wave (PW) Doppler, tissue Doppler imaging (TDI) and M-mode, respectively. No significant statistical differences were observed between the measured values and sex, heart rate and body weight. A positive correlation was observed between the maximum velocity of the right ventricular outflow tract and heart rate and also between TAPSE slope and body weight. With determining the normal values of PW-TDI of the right ventricle in apparently healthy domestic short-haired cats, it is expected that with providing normal reference values, the diagnosis of heart diseases especially asymptomatic heart disease be achieved as soon as possible to provide the best appropriate therapeutic management and monitoring decision about them.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 5","pages":"275-279"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/fb/vrf-14-275.PMC10278901.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9701023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of intracerebroventricular injection of apelin-13 on food intake in broiler chicks. 脑室注射apelin-13对肉鸡采食量的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.545646.3335

Razieh Amini Zadeh, Hossein Jonaidi, Saeed Esmaeili Mahani, Mahsa Salehi, Mojtaba Emam Bakhsh

{"title":"Effects of intracerebroventricular injection of apelin-13 on food intake in broiler chicks.","authors":"Razieh Amini Zadeh, Hossein Jonaidi, Saeed Esmaeili Mahani, Mahsa Salehi, Mojtaba Emam Bakhsh","doi":"10.30466/vrf.2022.545646.3335","DOIUrl":"https://doi.org/10.30466/vrf.2022.545646.3335","url":null,"abstract":"Apelin is an endogenous peptide ligand for G protein coupled apelin receptors (APJ orphan receptors) which are very similar to angiotensin II receptors. Apelin is expressed in most tissues of the body including hypothalamus that is responsible for regulating water and food intake, the gastrointestinal tract, the circulatory system, adipose and muscle tissues, and the immune system. The physiological actions of apelin, including food intake, has not yet been reported in birds. In this study, the effect of intracerebroventricular injection of different doses of apelin-13 was investigated on food intake in neonatal broilers at the age of five and seven days. The chicks had access to food immediately after injection and cumulative food intake was measured at half, 1, 2, 3, 4, 8 and 21 hr after injection. The 2-way ANOVA analyzed data showed that apelin-13 at dose of 1.00 μg significantly reduced food intake at 21 hr after injection in five-day old chicks. In addition, in dose of 1.50 μg, it could significantly reduce food intake at 2, 3, 4, 8 and 21 hr after injection. In seven-day-old chicks, the doses of 1.00 and 4.00 μg of apelin-13 had no effect on food intake compared to the control group. Apelin-13 at dose of 2.00 μg significantly reduced food intake at 8 and 21 hr after injection. The results of this study showed that apelin-13 had a reducing effect on food consumption in neonatal broiler chicks.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 2","pages":"105-108"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/b8/vrf-14-105.PMC10003593.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9100410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Configuration of sacral plexuses in Hasmer and Hasak sheep breeds: a comparative macro-anatomical approach. Hasmer和Hasak羊品种的骶神经丛结构:比较宏观解剖学方法。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2021.539003.3229

Hulya Balkaya, Zekeriya Ozudogru, Dervis Dervis, Bumin Emre Teke, Hulya Kara

{"title":"Configuration of sacral plexuses in Hasmer and Hasak sheep breeds: a comparative macro-anatomical approach.","authors":"Hulya Balkaya, Zekeriya Ozudogru, Dervis Dervis, Bumin Emre Teke, Hulya Kara","doi":"10.30466/vrf.2021.539003.3229","DOIUrl":"https://doi.org/10.30466/vrf.2021.539003.3229","url":null,"abstract":"The aim of this study was to examine and compare the macro-anatomical structures and configurations of plexus sacralis in Hasmer and Hasak sheep breeds. For this purpose, 18 sheep (eight female Hasmer sheep, three male Hasak sheep and seven female Hasak sheep) were assessed. After induction of anesthesia, the body cavities were opened. The sheep were fixed with formaldehyde after draining of the blood. The nerves of the plexus sacralis were dissected separately and photographed. Both in Hasmer and Hasak sheep breeds, the plexus sacralis was a large anastomotic complex of nerves which gave rise to nerves distributed to pelvic cavity and pelvic limb. It was derived from the ventral branches of the six spinal segments. It was observed that this plexus was formed by the ventral rami of four (first to fourth) sacral spinal nerves as well as ventral ramus of the sixth and fifth lumbar nerves. The results of this study are believed to be improved further with the future morphometric studies and to make a contribution to future anatomical and surgical studies in this area.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 2","pages":"65-69"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/93/vrf-14-65.PMC10003599.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9100416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the relationship between inflammatory reaction and interleukins in ovine pulmonary adenocarcinomas. 羊肺腺癌炎症反应与白细胞介素关系的评价。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.542311.3279

Emin Karakurt, Nüvit Coşkun, Enver Beytut, Ömer Faruk Keleş, Serpil Dağ, Volkan Yılmaz, Hilmi Nuhoğlu, Ayfer Yıldız, Emre Kurtbaş

{"title":"Evaluation of the relationship between inflammatory reaction and interleukins in ovine pulmonary adenocarcinomas.","authors":"Emin Karakurt, Nüvit Coşkun, Enver Beytut, Ömer Faruk Keleş, Serpil Dağ, Volkan Yılmaz, Hilmi Nuhoğlu, Ayfer Yıldız, Emre Kurtbaş","doi":"10.30466/vrf.2022.542311.3279","DOIUrl":"https://doi.org/10.30466/vrf.2022.542311.3279","url":null,"abstract":"In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 - 2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 - 2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor-associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 1","pages":"1-6"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/d1/vrf-14-1.PMC9906612.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of canine distemper in dogs referred to Veterinary Hospital of Ferdowsi University of Mashhad, Mashhad, Iran. 伊朗马什哈德费尔多西大学兽医医院犬犬瘟热流行情况。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.541661.3269

Farzad Mousafarkhani, Ali Asghar Sarchahi, Hadi Mohebalian, Javad Khoshnegah, Mohammad Arbabi

{"title":"Prevalence of canine distemper in dogs referred to Veterinary Hospital of Ferdowsi University of Mashhad, Mashhad, Iran.","authors":"Farzad Mousafarkhani, Ali Asghar Sarchahi, Hadi Mohebalian, Javad Khoshnegah, Mohammad Arbabi","doi":"10.30466/vrf.2022.541661.3269","DOIUrl":"https://doi.org/10.30466/vrf.2022.541661.3269","url":null,"abstract":"Canine distemper virus (CDV) is responsible for high morbidity and mortality in dogs worldwide. Epidemiological study of canine distemper can help to control and treat the disease in any area. This study aimed to investigate the prevalence of CDV in dogs referred to the Veterinary Hospital from September 23, 2018 to September 22, 2019. Dogs with at least two clinical signs of canine distemper underwent blood tests, rapid test kit from the eye and cerebrospinal fluid (CSF), and RT-PCR from whole blood and/or CSF samples. Out of 1212 referred dogs, 112 dogs were suspected to have canine distemper of which 90 underwent RT-PCR and rapid test kits. The disease prevalence was 4.04% (49/1212) and 7.44% (49/659) according to the total number of referring dogs and number of referring sick dogs, respectively. The distemper fatality rate was 69.57% (32/46). Seventy percent of distemper positive cases were under 12 months old and 52.08% were under 6 months old. Female dogs were more susceptible than males; however, the fatality rate of males was more than females. Of distemper positive dogs, 91.84% were unvaccinated. The highest prevalence (71.43%) of dogs diagnosed with CDV occurred during the cold seasons. It is concluded that canine distemper is endemic in the geographical area of Mashhad and its prevalence rate in dogs referred to the Veterinary Hospital of Ferdowsi University of Mashhad is 4.04% and its fatality rate is 69.57%. This indicates that a significant number of dogs may die if they develop distemper despite treatment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 3","pages":"153-160"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/db/vrf-14-153.PMC10073810.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9326065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review and meta-analysis of the use of plant essential oils and extracts in the development of antimicrobial edible films for dairy application. 植物精油和提取物在乳品用抗菌食用膜开发中的应用的系统综述和荟萃分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.1986122.3730

Houshmand Sharafi, Mehran Moradi, Kiomars Sharafi

{"title":"A systematic review and meta-analysis of the use of plant essential oils and extracts in the development of antimicrobial edible films for dairy application.","authors":"Houshmand Sharafi, Mehran Moradi, Kiomars Sharafi","doi":"10.30466/vrf.2022.1986122.3730","DOIUrl":"https://doi.org/10.30466/vrf.2022.1986122.3730","url":null,"abstract":"The purpose of this review was to assess the use of plant essential oils and extracts (PEOE) in the development of antimicrobial edible films for dairy application through a systematic review and meta-analysis. All studies published in multiple databases were explored via PRISMA protocol on November 1, 2022. According to the results, the interquartile range of pathogen reduction potential of essential oil (EO) in dairy products, irrespective of EO, film and product type, was 0.10 - 4.70 log CFU g-1 per % concentration. The findings from 38 articles indicate that among all EOs or their compounds, Zataria multiflora Boiss in protein film, thyme in protein film, Z. multiflora Boiss EO in protein film, Trans-cinnamaldehyde in carbohydrate film and lemongrass EO in protein film had extraordinary pathogen reduction potential on important foodborne pathogens. In the case of plant extract, fish gelatin film with Lepidium sativum extract, whey protein isolate film loaded with oregano EO and carboxymethyl cellulose film with clove EOs had the highest antimicrobial effect on mesophilic bacteria (9.50 log CFU g-1 per % concentration), yeast-mold (2.63 log CFU g-1 per % concentration) and mesophilic/ psychrophilic counts (> 9.06 log CFU g-1 per % concentration), respectively. Listeria monocytogenes is the primary species of interest; whereas, mesophiles and mold-yeast populations were the most investigated microbiota/mycobiota in cheese with PEOE-incorporated film. In light of these findings, the choice of PEOE at appropriate concentrations with the selection of appropriate edible film may improve the safety, sensory, and shelf life of dairy products.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 4","pages":"179-194"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/e1/vrf-14-179.PMC10170464.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9468686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen. 添加蒺藜水提物对犬精液冷藏过程中精子特性及抗氧化状态的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.546509.3347

Mohammad Moein Sabzeie, Esmail Ayen, Ali Soleimanzadeh, Mustafa Numan Bucak

{"title":"Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen.","authors":"Mohammad Moein Sabzeie, Esmail Ayen, Ali Soleimanzadeh, Mustafa Numan Bucak","doi":"10.30466/vrf.2022.546509.3347","DOIUrl":"https://doi.org/10.30466/vrf.2022.546509.3347","url":null,"abstract":"The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The current study looks at the possible advantages of chilling canine sperm to 4.00 ˚C for three days using Tribulus terrestris aqueous extract (TTAE). Three mixed-breed dogs were utilized to extract 24 ejaculates, which were then diluted in a Tris-based extender. The ejaculates were then divided into five groups including 20.00, 40.00 and 50.00 µg mL-1 of TTAE, sham (distilled water devoid of TTAE) and control (without TTAE) groups. During the three days of experiment, several parameters were measured every 24 hr. It was noticed that after 48 and 72 hr of liquid storage, total and progressive motilities were greater in the group with the 40.00 µg mL-1 TTAE concentration than the control group. Compared to the control group, the group with the 40.00 µg mL-1 TTAE concentration exhibited superior motility and viability. The percentages obtained from the hypo-osmotic swelling test were much greater. In contrast to the control group, DNA integrity was poorer in the 40.00 µg mL-1 TTAE concentration. After 72 hr of storage, the group with 40.00 µg mL-1 TTAE concentration had lower malondialdehyde levels but considerably greater total anti-oxidant capacity, superoxide dismutase, glutathione peroxidase and catalase levels than the control groups. The current study found that supplementing the semen extender with 40.00 µg mL-1 TTAE improves semen parameters after 72 hr of storage at 4.00 ˚C, and therefore can improve fertilization efficiency.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 2","pages":"71-77"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/8a/vrf-14-71.PMC10003598.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9470069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of pyrethroids resistance alleles in goat biting louse Bovicola caprae (Phthiraptera: Trichodectidae) in west and northwest of Iran. 伊朗西部和西北部山羊咬虱Bovicola caprae（Phthiraptera:毛滴虫科）中拟除虫菊酯类抗性等位基因的检测。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-09-15 DOI: 10.30466/vrf.2023.556815.3565

Khadijeh Seydi Gazafi, Mousa Tavassoli, Karim Mardani

{"title":"Detection of pyrethroids resistance alleles in goat biting louse Bovicola caprae (Phthiraptera: Trichodectidae) in west and northwest of Iran.","authors":"Khadijeh Seydi Gazafi, Mousa Tavassoli, Karim Mardani","doi":"10.30466/vrf.2023.556815.3565","DOIUrl":"10.30466/vrf.2023.556815.3565","url":null,"abstract":"Pyrethroid insecticides target voltage-gated sodium channels (VGSCs) that are essential for electrical signaling in the nervous system of insects. Three-point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F located in domain II conferring the knockdown resistance (kdr) are the most important mutations in pyrethroid-resistant lice worldwide. In addition, six new mutations have been reported in the extracellular loops IIS1-2 (H813P) and IIS5 (I927F, L928A, R929V, L930M, L932M) in the α- subunit of the sodium channel in lice. The aim of this study was to detect alleles resistant to pyrethroids in the domain II (S5-S6) of the VGSC gene in goat biting louse. Goat biting lice were collected from five provinces in the west and northwest of Iran. Genomic DNA was extracted from goat biting lice and Bovicola (Damalinia) caprae species was confirmed by amplifying the cytochrome oxidase subunit I (COXI) gene. A fragment in the domain II (S5-S6) of the VGSC gene was amplified using the specific primers and the resultant polymerase chain reaction products were sequenced. Substitutions T917I, L920F, I927F, L928A, R929V and L930M were identified in the examined sequences. The results showed that all the examined lice had at least one mutation in their VGSC gene associated with pyrethroid resistance or new mutations. The presence of these mutated alleles in the VGSC gene may be due to the long-term and multiple use of pyrethroids against arthropods. Thus, the molecular detection of resistance to pyrethroid insecticides in goat chewing lice can help plot a kdr frequency map to enact effective policies to control caprine pediculosis.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 9","pages":"489-494"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/fb/vrf-14-489.PMC10560324.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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