Veterinary Research Forum最新文献_第10页

Evaluation of porcine circovirus type 2 double vaccination in weaning piglets that reared for gilts under field conditions. 猪圆环病毒2型双重接种在猪场条件下对后备仔猪的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.543079.3292

Vasileios Papatsiros, Georgios Papakonstantinou, Eleftherios Meletis, Dimitra Bitchava, Polychronis Kostoulas

{"title":"Evaluation of porcine circovirus type 2 double vaccination in weaning piglets that reared for gilts under field conditions.","authors":"Vasileios Papatsiros, Georgios Papakonstantinou, Eleftherios Meletis, Dimitra Bitchava, Polychronis Kostoulas","doi":"10.30466/vrf.2022.543079.3292","DOIUrl":"https://doi.org/10.30466/vrf.2022.543079.3292","url":null,"abstract":"The objective of the present study was to evaluate the efficacy of a porcine circovirus type 2 (PCV2) double vaccination in weaning piglets reared for gilts under field conditions. The study was conducted at a Greek farrow-to-ﬁnish conventional pig farm with a previous history of PCV2 infections. The trial included 96 female piglets at 21 days of age, which were equally allocated to two different study groups. Piglets of the group-1 received a single PCV2 vaccination at 21 days of age, while piglets of the group-2 were double vaccinated against PCV2, at 21 and 42 days of age. The results indicated that the piglets of group-2 had better growth performance, as they showed higher body weight (BW) and average daily weight gain (ADWG). In addition, ELISA tests showed that the double- vaccinated piglets presented a better humoral response against PCV2, as higher levels of IgG antibodies were detected in them than the piglets of the group-1. In conclusion, the current results suggested that a double PCV2 vaccination of piglets, reared for gilts, on a PCV2-affected farm could lead to higher protection against the virus.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/89/2e/vrf-14-13.PMC9906616.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary astaxanthin (Lucantin^® Pink) mitigated oxidative stress induced by diazinon in rainbow trout (Oncorhynchus mykiss). 饲料中虾青素(Lucantin®Pink)可减轻虹鳟(Oncorhynchus mykiss)氧化应激。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2021.533582.3209

Sadigheh Shabanzadeh, Saber Vatandoust, Seyed Mehdi Hosseinifard, Najmeh Sheikhzadeh, Amir Ali Shahbazfar

{"title":"Dietary astaxanthin (Lucantin® Pink) mitigated oxidative stress induced by diazinon in rainbow trout (Oncorhynchus mykiss).","authors":"Sadigheh Shabanzadeh, Saber Vatandoust, Seyed Mehdi Hosseinifard, Najmeh Sheikhzadeh, Amir Ali Shahbazfar","doi":"10.30466/vrf.2021.533582.3209","DOIUrl":"https://doi.org/10.30466/vrf.2021.533582.3209","url":null,"abstract":"The potential of commercial astaxanthin on growth, biochemical factors, and antioxidant-related gene expression following a challenge with diazinon were studied in rainbow trout (Oncorhynchus mykiss). Fish (~ 20.70 g) were fed diets containing commercial astaxanthin (ASX) at 0.00 (CTR and ASX0), 0.50 (ASX1), 2.00 (ASX2), and 5.00 (ASX3) g kg-1 for 60 days. Afterwards, the treated fish (ASX1, ASX2, ASX3) as well as the fish in ASX0 group were challenged with diazinon (0.11 mg L-1) for 96 hr whereas fish in the CTR group was not challenged with diazinon. Results showed that growth pattern improved significantly with all enriched diets compared to the ASX0 group. Metabolic enzyme activities, including alanine aminotransferase and alkaline phosphatase decreased in ASX2 and ASX3 groups with respect to the ASX0 group. Serum antioxidant status also showed the same pattern with enhancement in the fish fed with the ASX2 and ASX3 supplemented diets. Feeding the fish with astaxanthin, particularly in the ASX3 group, up-regulated the expression of some antioxidant-relevant genes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and nuclear factor erythroid-2 related factor 2 (Nrf2) in the kidney and liver. Besides, the histopathological damages in kidneys and liver induced by diazinon were less pronounced in the ASX2 and ASX3 groups compared to the ASX0 group. In conclusion, commercial astaxanthin, especially at 5.00 g kg-1, enhanced the growth performance and ameliorated the oxidative stress induced by diazinon in rainbow trout.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/4f/vrf-14-97.PMC10003594.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9470068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Efficacy of light chain 3-fused protein multi epitope in protection of mice challenged with Mycobacterium tuberculosis. 轻链 3 融合蛋白多表位在保护小鼠免受结核分枝杆菌感染方面的功效。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-12-15 DOI: 10.30466/vrf.2023.1975747.3702

Somayeh Bahrami, Mohammad Mehdi Feizabadi, Nader Mosavari, Fattah Sotoodehnejad, Mohammad Eslampanah

{"title":"Efficacy of light chain 3-fused protein multi epitope in protection of mice challenged with Mycobacterium tuberculosis.","authors":"Somayeh Bahrami, Mohammad Mehdi Feizabadi, Nader Mosavari, Fattah Sotoodehnejad, Mohammad Eslampanah","doi":"10.30466/vrf.2023.1975747.3702","DOIUrl":"10.30466/vrf.2023.1975747.3702","url":null,"abstract":"The new strategy for vaccine development such as the fused protein multi-epitope capable of preventing the reactivation of latent tuberculosis infection (LTBi) can be an effective strategy for controlling tuberculosis (TB) worldwide. This study was conducted to evaluate the immunity of experimentally infected BALB/c mice with Mycobacterium tuberculosis after injection of DNA construct. Nineteen female BALB/c mice were divided into three groups and injected with 0.50 mL of M. tuberculosis. After 3 weeks, lung and spleen samples from the infected mice were examined. The protective effects of light chain 3-fused protein multi-epitope against TB were evaluated for post-exposure and therapeutic exposure. The lungs and spleens of the mice were aseptically removed after death for histopathology analysis. The bacterial colonies were counted, and the cells were stained after 3 weeks of incubation. No significant differences were observed between the post-exposure and therapeutic exposure groups. The pathological changes in the lung tissue of mice in these groups included an increase in the thickness of interalveolar septa, hyperemia, and intraparenchymal pulmonary hemorrhage centers (positive control), scattered hyperemic areas (negative control), and hyperemia in the interstitial tissue, scattered hyperemic areas in the lung parenchyma and lymphocytic infiltration centers (experimental group). Flow cytometry of the post-exposure and therapeutic exposure models showed insignificant changes in all three groups. It seems necessary to develop a post-exposure and therapeutic exposure vaccine strategy that focuses on LTBi to prevent the progression of the active disease. In this regard, multi-epitope vaccines should be designed to induce both cellular and humoral immunity.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebellar fastigial nucleus histamine and its H₂ but not H₁ receptors might inhibit acetic acid-induced visceral nociception and improve motor coordination in rats: role of opioid system. 小脑顶核组胺及其H2而非H1受体可能抑制乙酸诱导的大鼠内脏伤害感受并改善运动协调：阿片系统的作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-10-15 DOI: 10.30466/vrf.2023.1988302.3762

Fereshteh Anbarian, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad

{"title":"Cerebellar fastigial nucleus histamine and its H2 but not H1 receptors might inhibit acetic acid-induced visceral nociception and improve motor coordination in rats: role of opioid system.","authors":"Fereshteh Anbarian, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2023.1988302.3762","DOIUrl":"10.30466/vrf.2023.1988302.3762","url":null,"abstract":"The cerebellum and its deep nuclei contribute to the regulation of important functions including motor coordination and pain. Histamine modulates some functions of the fastigial nucleus (FN) such as motor coordination. In this study, by application of histamine and activation of its H1 and H2 receptors, the FN processing of visceral pain, general locomotor activity and motor coordination were targeted. The possible mechanism of action was followed by the inhibition of opioid receptors. The right and left sides of the FN were surgically implanted with guide cannulas. Immediately after an intraperitoneal injection of acetic acid (1.00 mL, 1.00%), the first writhing onset latency and the writhing number over 60 min were recorded. Open-field and rotarod tests were applied for general locomotor and motor coordination assessment, respectively. Histamine and dimaprit (H2 receptor agonist) increased first writhing onset latency, decreased the writhing number and increased falling time from the rod. These effects were prevented by ranitidine (H2 receptor antagonist) pre-treatment. Significant alterations were not observed by histamine H1 receptor agonist (2-pyridylethylamine) and antagonist (mepyramine). Naloxone, with no effect on falling time from the rod, inhibited the antinociceptive effects of histamine and dimaprit. Beam break number was not affected by the above-mentioned treatments. Based on the results, it can be suggested that histamine H2, but not H1 receptors at the FN might have had an inhibitory role on acetic acid-induced visceral pain and improved motor coordination. The antinociception, but not motor coordination might be mediated by FN opioid receptors.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71414294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and genotypes of Giardia duodenalis in shelter dogs of southeastern Türkiye. 土耳其东南部收容所犬只中十二指肠贾第虫的流行率和基因型。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-11-15 DOI: 10.30466/vrf.2023.1998950.3826

Burçak Aslan Çelik, Özgür Yaşar Çelik, Akın Koçhan, Adnan Ayan, Özlem Orunç Kılınç, Gürkan Akyıldız, Kıvanç İrak, Özge Oktay Ayan, Kerem Ercan

{"title":"Prevalence and genotypes of Giardia duodenalis in shelter dogs of southeastern Türkiye.","authors":"Burçak Aslan Çelik, Özgür Yaşar Çelik, Akın Koçhan, Adnan Ayan, Özlem Orunç Kılınç, Gürkan Akyıldız, Kıvanç İrak, Özge Oktay Ayan, Kerem Ercan","doi":"10.30466/vrf.2023.1998950.3826","DOIUrl":"10.30466/vrf.2023.1998950.3826","url":null,"abstract":"Giardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10758005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of volatile compounds of anal sac secretions between the sexes of domestic dog (Canis lupus familiaris). 家犬(Canis lupus familiaris)肛囊分泌物挥发性化合物的两性比较。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2023.1983063.3714

Ibrahim Dorrigiv, Mojtaba Hadian, Morteza Bahram

{"title":"Comparison of volatile compounds of anal sac secretions between the sexes of domestic dog (Canis lupus familiaris).","authors":"Ibrahim Dorrigiv, Mojtaba Hadian, Morteza Bahram","doi":"10.30466/vrf.2023.1983063.3714","DOIUrl":"https://doi.org/10.30466/vrf.2023.1983063.3714","url":null,"abstract":"Volatile compounds of anal sac secretions are odorant chemicals used across the carnivores for social communication such as identifying individuals and group membership. Odor profiles taken from expressed anal sac secretions of some species of carnivores have been detected in previous studies. In this study, the volatile compounds of anal sac secretions between five male and five female domestic dogs (Canis lupus familiaris) were compared. Volatile chemicals were extracted, separated, and analyzed by gas chromatography-mass spectrometry with solid-phase micro-extraction and identified from their electron ionization mass spectra and Kovats retention indices. The results showed the presence of various types of compounds including organic fatty acids, ketones, aldehydes, esters, and alcohols in the anal sac secretions of dogs. Greater amounts of diversity and esters, and lower amounts of alcohols were detected in the anal sac secretions of females compared to males. This was accompanied by finding citrate and acetic acid ester only in the females. Furthermore, presence of some sex-specific organic compounds like dimethylcyclopentyl ethanone indicates that the volatile profiles of anal sac secretions in 10 domestic dogs are differentiated by host sex.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ed/e9/vrf-14-169.PMC10073811.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

First identification of Mycoplasma capricolum subspecies capripneumoniae in goats in Iran. 伊朗山羊山羊山羊支原体卡布利肺炎亚种的首次鉴定。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.555079.3496

Mostafa Abdollahi, Samad Lotfollahzadeh, Mohammad Hossein Nazem Shirazi, Sara Shokrpoor, Farhad Moosakhani, Minoo Partovi Nasr

{"title":"First identification of Mycoplasma capricolum subspecies capripneumoniae in goats in Iran.","authors":"Mostafa Abdollahi, Samad Lotfollahzadeh, Mohammad Hossein Nazem Shirazi, Sara Shokrpoor, Farhad Moosakhani, Minoo Partovi Nasr","doi":"10.30466/vrf.2022.555079.3496","DOIUrl":"https://doi.org/10.30466/vrf.2022.555079.3496","url":null,"abstract":"Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the etiological agent of caprine contagious pleuropneumonia (CCPP) disease. The CCPP is one of the most severe diseases of goats. A herd of 2,000 goats located in the countryside of Tehran city, Iran, was examined for the study in August 2021. In history taking, observation, inspection and clinical examination, high case fatality rate (46.00%) due to respiratory distress and high morbidity of pleuropneumonia (15.00%) syndrome were recorded. Accordingly, ten carcasses of goats were dissected. The epidemiological pattern of the disease, clinical examination findings and the signs of necropsy of dead patients were suspected to CCPP. Four lung samples of necropsied goats were sent to the laboratory for PCR test and in all of them, Mccp was detected and CCPP was also confirmed. The disease was controlled by two measures: (a) the whole herd was first treated with antibiotics (florfenicol and tylosin) and (b) then the Pulmovac-In vaccine was then administered. This study is the first documented report of CCPP occurrence caused by Mccp in Iran and shows the importance of availability of effective vaccines to control and prevention of CCPP.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/6a/vrf-14-109.PMC10003596.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9095998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Escherichia coli strain Nissle 1917 on arsenic-challenged goldfish (Carassius auratus): histological evidence. 大肠埃希菌Nissle 1917对砷致毒金鱼的影响:组织学证据。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.557449.3551

Katayoon Nofouzi, Najmeh Sheikhzadeh, Gholamreza Hamidian, Amir Ali Shahbazfar, Amin Marandi

{"title":"Effects of Escherichia coli strain Nissle 1917 on arsenic-challenged goldfish (Carassius auratus): histological evidence.","authors":"Katayoon Nofouzi, Najmeh Sheikhzadeh, Gholamreza Hamidian, Amir Ali Shahbazfar, Amin Marandi","doi":"10.30466/vrf.2022.557449.3551","DOIUrl":"https://doi.org/10.30466/vrf.2022.557449.3551","url":null,"abstract":"Arsenic (As) contamination in natural water resources has become a great disaster throughout the world posing serious health problems. The current study was performed to evaluate the protective effects of Escherichia coli strain Nissle 1917 (EcN) against As exposure in goldfish (Carassius auratus). Fish were fed three times a day with 4.00% of body weight of diet with different doses (0.00, 1.00 × 106, 1.00 × 107 and 1.00 × 108 CFU g-1) of EcN for 80 days and then, challenged with 20.00 mg L-1 As for 96 hr under stagnant flow. Physicochemical characteristics of the inlet water were temperature of 25.10 ± 0.70 ˚C, pH of 7.30 ± 0.20 and dissolved oxygen of 7.30 ± 0.30 mg L-1 and 50.00% of water was exchanged once a week. Afterwards, fish were euthanized with a clove oil solution (50.00 μL L-1) and tissues were dissected from each fish and immediately fixed in 10.00% buffered formalin. The histopathological results indicated that the supplemented EcN did not have any side effects on various organs. It was also observed that the damages to kidney, liver, gill and skin were pronounced in fish exposed to As. However, the histopathological damages induced by As in fish tissues were less pronounced in the EcN-treated groups compared to the fish fed with the basal diet. Lamellar blood congestion in gills and epidermal cells detachment from the skin surface as well as hepatocytes, enterocytes and tubular necrosis were reduced in treated groups. These findings indicate that EcN has the potential to ameliorate the As-induced organ toxicity.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/90/vrf-14-367.PMC10410110.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological lesions of Actinobacillus pleuropneumoniae serotype 8 in infected pigs. 感染猪胸膜肺炎放线杆菌血清8型的组织病理学病变。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.556971.3539

Vasileios Georgios Papatsiros, Ioanna Stylianaki, Constantina Nikolaos Tsokana, Georgios Papakonstantinou, Marios Christophorou, Nikolaos Papaioannou, Labrini Vasileios Athanasiou

{"title":"Histopathological lesions of Actinobacillus pleuropneumoniae serotype 8 in infected pigs.","authors":"Vasileios Georgios Papatsiros, Ioanna Stylianaki, Constantina Nikolaos Tsokana, Georgios Papakonstantinou, Marios Christophorou, Nikolaos Papaioannou, Labrini Vasileios Athanasiou","doi":"10.30466/vrf.2022.556971.3539","DOIUrl":"https://doi.org/10.30466/vrf.2022.556971.3539","url":null,"abstract":"This study aimed to report, for the first time, histopathological lesions caused by an outbreak of acute Actinobacillus pleuropneumoniae serotype 8 infections in two farms in Cyprus. Lung tissue samples were collected from two different affected farms (a total of eight samples) for bacterial culture, multiplex polymerase chain reaction (PCR)-based serotyping and histopathological evaluation. Severe respiratory clinical signs, vomiting, anorexia, sudden deaths, a morbidity rate of around 25.00% and a mortality rate of over 60.00% in the fattening stage were reported. Macroscopic lesions included acute to subacute fibrotic, hemorrhagic and necrotizing pneumonia with occasionally encapsulated nodule-like abscesses and fibrous pleuritis. Histopathological evaluation revealed fibrous exudate between alveolar spaces and connective tissue, areas of necrosis mixed with alveolar macrophages, lymphocytes, plasma cells and necrotic leukocytes surrounding colonies of cocci. The bronchial and bronchiolar epithelia were degenerated and replaced by eosinophilic cell debris mixed with inflammatory cells. Several arteries and capillaries were clotted and/or infiltrated by inflammatory cells. In conclusion, these A. pleuropneumoniae serotype 8 cases were accompanied by acute illness, death and more pronounced bronchitis and bronchiolitis.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/dd/vrf-14-401.PMC10410112.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of bovine leukosis virus in naturally infected dairy and dual-purpose cattle in Mexico. 墨西哥天然感染奶牛和两用牛中牛白血病病毒的分子检测。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.555834.3510

Rosa María Cordero-Pulido, David Itzcóatl Martínez-Herrera, Héctor Vivanco-Cid, José Alfredo Villagómez-Cortés, Maja Louise Arendt, Peter Grube-Pagola, Carlos Alonso Domínguez-Alemán

{"title":"Molecular detection of bovine leukosis virus in naturally infected dairy and dual-purpose cattle in Mexico.","authors":"Rosa María Cordero-Pulido, David Itzcóatl Martínez-Herrera, Héctor Vivanco-Cid, José Alfredo Villagómez-Cortés, Maja Louise Arendt, Peter Grube-Pagola, Carlos Alonso Domínguez-Alemán","doi":"10.30466/vrf.2022.555834.3510","DOIUrl":"https://doi.org/10.30466/vrf.2022.555834.3510","url":null,"abstract":"The objective of this study was to determine the prevalence of bovine leukosis virus (BLV) in specialized and dual-purpose dairy cows located in the central zone of Veracruz state in Mexico, using endpoint polymerase chain reaction (PCR). The study population consisted of 307 specialized dairy cows and 95 dual-purpose cows from 13 municipalities located in the study area. All cows were apparently healthy and ≥ 3 years old. Cows were stratified by age (3 - 5, 6 - 8 and ≥ 9 years). The overall prevalence of infection was 6.96%; the calculated prevalence in dairy cows was 7.82% and in dual-purpose cows it was 4.21%. The municipality with the highest proportion was Acajete (14.28%), followed by Huatusco and Tomatlán (11.53%). The association analysis confirms the infection's independence to the cows' productive purpose. The results by age strata were 3 - 5 (4.60%), 6 - 8 (8.00%) and ≥ 9 (18.40%) with X2 = 9.96, with an odds ratio of 4.68 for the stratum ≥ 9 years with a significant difference. The present study determined the prevalence of proviral DNA of BLV in dairy and dual-purpose cows in six municipalities in the central zone of Veracruz state, Mexico, using endpoint PCR.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/22/09/vrf-14-457.PMC10475164.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10217297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0