Veterinary Research Forum最新文献_第10页

Copper and zinc dynamics in ovine pneumonia: a comparative analysis of treatment regimens. 羊肺炎中铜和锌的动态：治疗方案的比较分析。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2043423.4496

Amir Ganjkhanloo, Bahram Dalir-Naghadeh, Ghader Jalilzadeh-Amin, Siamak Asri-Rezaei

{"title":"Copper and zinc dynamics in ovine pneumonia: a comparative analysis of treatment regimens.","authors":"Amir Ganjkhanloo, Bahram Dalir-Naghadeh, Ghader Jalilzadeh-Amin, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2043423.4496","DOIUrl":"10.30466/vrf.2024.2043423.4496","url":null,"abstract":"Pneumonia remains a significant economic burden on the small ruminant industry. Excessive inflammation, oxidative stress, and alterations in copper and zinc can accompany pneumonia. As these micro-nutrients play crucial roles in immune function and anti-oxidant defence, modulating their levels may influence the disease progression. This study aimed to investigate the effects of different therapeutic regimens on copper and zinc status in lambs with pneumonia. Twenty lambs with pneumonia were randomly assigned to four treatment groups: oxytetracycline and tylosin (OT), OT plus vitamin B1 (OTVB1), OT plus vitamin C (OTVC), and OT plus vitamin B1 and vitamin C (OTVB1C). A control group received only distilled water. Blood samples were collected on days 1, 3, 6, and 14 for subsequent assessment of plasma copper and zinc concentrations. While the control group maintained stable levels, the pneumonic groups exhibited varying degrees of changes. Plasma copper concentrations increased significantly in all pneumonic groups compared to the control group throughout the study. The OT and OTVB1C groups had the highest number of lambs with increased copper level. Plasma zinc concentrations decreased significantly in the OT and OTVB1 groups, with the lowest levels in the OTVB1 group on day 3. The OTVC group mirrored the control group with stable levels. The OTVB1C group, compared to the other groups, showed a more persistent reduction. These findings suggest that the effects of the various treatment regimens on plasma copper and zinc levels may be complex and time-dependent.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"331-338"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic regulation of apoptotic and antioxidant pathways throughout the reproductive cycle in female blue swimmer crab. 雌性蓝泳蟹生殖周期中凋亡和抗氧化途径的动态调控。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2029765.4289

Shabnam Adhami, Negin Salamat, Ahmad Savari, Gholamreza Hamidian

{"title":"Dynamic regulation of apoptotic and antioxidant pathways throughout the reproductive cycle in female blue swimmer crab.","authors":"Shabnam Adhami, Negin Salamat, Ahmad Savari, Gholamreza Hamidian","doi":"10.30466/vrf.2024.2029765.4289","DOIUrl":"10.30466/vrf.2024.2029765.4289","url":null,"abstract":"This study investigated the antioxidant and apoptotic systems of blue swimmer crabs at various reproductive stages, providing valuable insights into their potential as biological markers, particularly in the polluted Persian Gulf. Our research along the coasts of Hendijan County, Iran, involved capturing live crabs (167 ± 52.07 g), examining their morphological traits and determining their reproductive stages through dissection and histological analysis. Apoptosis was detected using the TUNEL assay (terminal deoxynucleotidyl transferase dUTP nick end labeling), and enzyme activities including superoxide dismutase, catalase and glutathione peroxidase were measured using colorimetric methods. Variations were observed in the abundance of apoptotic cells within the hepatopancreas across reproductive stages. The second stage exhibited the lowest values and the first stage displayed the highest indicating a potential link between reproductive activity and apoptosis. Furthermore, enzymes representing the antioxidant system demonstrated various activities during ovarian development. Notably, the second ovarian stage demonstrated the highest catalase (5.63 mM per g protein) and malondialdehyde (12.14 mM per g protein) activities indicating an elevated response to oxidative stress. Our findings demonstrated that apoptotic cell numbers were fluctuated throughout the reproductive stages in the crabs, with the highest levels observed during the first stage and the lowest during the second stage. Understanding these fluctuations not only aids in distinguishing between reproductive and non-reproductive phases but also offers valuable insights into the broader physiological changes occurring throughout the cycle.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"353-360"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coxiella burnetii in dogs and ticks from the Northeastern Anatolia region of Türkiye: serological and molecular findings. <s:1>基耶省东北部安纳托利亚地区犬和蜱中的伯纳克谢氏菌：血清学和分子学发现。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-11 DOI: 10.30466/vrf.2024.2035315.4359

Aliye Gülmez Sağlam, Elif Çelik, Fatih Büyük, Gencay Taşkın Taşçi, Seda Gökdemir, Nilgün Aydin, Eray Büyük, Salih Otlu

{"title":"Coxiella burnetii in dogs and ticks from the Northeastern Anatolia region of Türkiye: serological and molecular findings.","authors":"Aliye Gülmez Sağlam, Elif Çelik, Fatih Büyük, Gencay Taşkın Taşçi, Seda Gökdemir, Nilgün Aydin, Eray Büyük, Salih Otlu","doi":"10.30466/vrf.2024.2035315.4359","DOIUrl":"10.30466/vrf.2024.2035315.4359","url":null,"abstract":"The Q fever is a zoonotic bacterial infection caused by an obligate intra-cellular bacterium, Coxiella burnetii. Members of the Canidae family (Mammalia), including dogs and foxes, are potential reservoirs of C. burnetii, which has a wide host range from mammals and birds to arthropods (primarily ticks). Infected dogs can transmit the disease to other animals and humans. This study aimed to investigate the presence of C. burnetii in dogs and ticks collected from infested dogs in the Kars, Ardahan, and Iğdir provinces of Türkiye by serological and molecular methods. Three hundred canine serum samples were analyzed for phase I and phase II C. burnetii antibodies using indirect enzyme-linked immunosorbent assay. Whole blood samples (n = 300) from the dogs sampled for sera and 184 ticks randomly collected from these dogs were also analyzed for C. burnetii with touch-down polymerase chain reaction. The ticks were classified according to the taxonomic characteristics. In result, 107 tick DNA samples collected from individual females and pooled males were evaluated. The C. burnetii was detected in 3.73% (of the tick samples. However, C. burnetii was not detected in any of the canine blood samples by polymerase chain reaction. Out of the 300 dogs, 18.33% presented antibodies against C. burnetii in their blood serum. When assessed for location, C. burnetii seropositivity was found to be significantly high especially in the Northeastern Anatolia region (18.33%). Study data highlighted the zoonotic risk of ticks, demonstrating that ticks on dogs can carry C. burnetii.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"431-437"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of tannin-degrading bacteria isolated from the rumen of some ruminants on the in vitro digestibility and gas production of fruits residues silage. 反刍动物瘤胃单宁降解菌对水果残渣青贮体外消化率和产气量的影响

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2025.2038543.4405

Maryam Gheibipour, Seyyed Ehsan Ghiasi, Seyed Morteza Vaghar Seyedin, Amir Zeidi, Hossein Motamedi

{"title":"Effect of tannin-degrading bacteria isolated from the rumen of some ruminants on the in vitro digestibility and gas production of fruits residues silage.","authors":"Maryam Gheibipour, Seyyed Ehsan Ghiasi, Seyed Morteza Vaghar Seyedin, Amir Zeidi, Hossein Motamedi","doi":"10.30466/vrf.2025.2038543.4405","DOIUrl":"10.30466/vrf.2025.2038543.4405","url":null,"abstract":"While severe shortage of feed has presented livestock industry with a major challenge, millions of tons of fruit by-products (FBPs) are discarded annually, as valuable sources of energy and numerous nutrients. However, some of FBPs contain high tannins that must be treated for use in animal feeding. This study was investigated the effect of tannin-degrading bacteria isolated from the rumen of some ruminants on the in vitro digestibility and gas production of FBPs silage. The FBPs, including pomegranate peel (PP), and the pulps of lemon (LP), grape (GP), and orange (OP) were ensilaged with tannin-degrading bacteria (enzyme activity: 10.46 - 8.60 U mL-1) isolated from the rumen of male goat (Escherichia coli GHMGHE41), deer (Escherichia fergusonii GHMGHE44), ram (E. fergusonii GHMGHE30), and camel (Klebsiella aerogenes GHMGHE38). After anaerobic incubation (30 days; 39.00 ˚C), PP + camel strain silage showed the highest dry matter, and the lowest natural acid detergent fiber and pH. The camel strain increased crude protein content of LP silage to the highest level, and decreased acid detergent fiber of GP silage to the lowest level. The highest digestibility was observed for LP + goat strain silage (50.37%) compared to the uninoculated OP silage (42.73%). The maximum ammonia (N-NH3) and minimum level of pH were recorded for the silages of LP + goat strain and PP + CR strain, respectively. Overall, the current results showed that tannin-degrading E. coli GHMGHE41 and K. aerogenes GHMGHE38 were able to improve the digestibility of LP and PP silages as ingredients in ruminants' diets.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"455-464"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of erythropoietin on cardiac morphometry in exercised male and female adolescent rats. 促红细胞生成素对运动后雌雄大鼠心脏形态的影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2035267.4360

Mehmet Burak Ateş, Gokhan Akcakavak, Ozgur Ozdemir, Mehmet Ozdemir, Ibrahim Bozkurt

{"title":"Effects of erythropoietin on cardiac morphometry in exercised male and female adolescent rats.","authors":"Mehmet Burak Ateş, Gokhan Akcakavak, Ozgur Ozdemir, Mehmet Ozdemir, Ibrahim Bozkurt","doi":"10.30466/vrf.2024.2035267.4360","DOIUrl":"10.30466/vrf.2024.2035267.4360","url":null,"abstract":"Erythropoietin (EPO) is a glycoprotein hormone predominantly produced in the kidneys, primarily stimulating erythroid cell proliferation in the bone marrow. The present study investigated the impact of EPO combined with swimming exercise on cardiac morphometry in adolescent male and female rats. The 4-week study involved 48 rats (24 males and 24 females), which were divided into four main groups of six males and six females each. The control group was administered intraperitoneal saline four times a week. The swimming exercise group also received intraperitoneal saline, followed by 30 min of swimming exercise, four times a week. The drug control group was given 50.00 IU kg-1 epoetin alfa intraperitoneally, four times a week. Lastly, the Swimming + Drug group received 50.00 IU kg-1 epoetin alfa intraperitoneally, four times a week, followed by 30 min of swimming exercise. The post-study measurements demonstrated that EPO administration did not result in notable alterations in crucial parameters, including the left ventricular mass index, left ventricular mass, and left ventricular posterior wall in the context of left ventricular hypertrophy in both genders. However, in female rats, EPO-only group and the combined EPO and exercise group showed significant thinning of the right ventricular wall and interventricular septum indicating potential cardiac dilatation. The results highlight the necessity of considering gender-specific responses when evaluating EPO's cardiovascular effects, particularly concerning the right ventricle, and suggest further investigation into the long-term consequences of these observed changes.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"189-194"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of effects of vancomycin/polycaprolactone nanocomposite in comparison with curcumin/polycaprolactone on the healing of experimental osteomyelitis in rabbit tibia. 万古霉素/聚己内酯纳米复合材料与姜黄素/聚己内酯纳米复合材料对兔胫骨实验性骨髓炎愈合的影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.30466/vrf.2024.2028501.4268

Amirreza Hajati Ziabari, Alireza Jahandideh, Abolfazl Akbarzadeh, Pejman Mortazavi

{"title":"Evaluation of effects of vancomycin/polycaprolactone nanocomposite in comparison with curcumin/polycaprolactone on the healing of experimental osteomyelitis in rabbit tibia.","authors":"Amirreza Hajati Ziabari, Alireza Jahandideh, Abolfazl Akbarzadeh, Pejman Mortazavi","doi":"10.30466/vrf.2024.2028501.4268","DOIUrl":"10.30466/vrf.2024.2028501.4268","url":null,"abstract":"Osteomyelitis is caused by the local spread of an infected source adjacent to the infection following trauma, bone surgery or joint replacement. The present study aimed to evaluate the effect of vancomycin (Van)/polycaprolactone (PCL) nanocomposite in comparison with curcumin (Cur)/PCL on the healing of experimental osteomyelitis in tibia in rabbits. After induction of osteomyelitis forty adult male New Zealand white rabbits were randomly divided into four groups. Control group: The animals were considered as controls and no scaffolds were used. In PCL/Van group, the created bone defects were filled with the combination of PCL and Van. In PCL/Cur, the created bone defects were filled with the combination of PCL and Cur. Polycaprolactone/Cur/Van group: The created bone defects were filled with the combination of PCL, Cur and Van. Bone samples were taken for histopathological evaluation on the 30th and 60th days. For radiological evaluations of the osteomyelitis, radiographs were prepared at time intervals zero (day of surgery), 15, 30, 45, and 60 days after surgery. In order to evaluate hematology, blood was taken on days 0 (day of surgery), 15, 30, 45, and 60. The results of the present study showed that Cur nanocomposite significantly improved the healing process of the rabbit tibia experimental osteomyelitis model compared to the control group. Also, the PCL/Cur/Van group showed the best healing results. In conclusion, PCL/Cur/Van nanocomposite scaffold showed positive effects on the healing process.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"235-243"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital. 西班牙一家兽医教学医院从环境和兽医人员中分离出的葡萄球菌的流行率和抗微生物耐药性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2016107.4086

Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez

{"title":"Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital.","authors":"Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez","doi":"10.30466/vrf.2024.2016107.4086","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016107.4086","url":null,"abstract":"Methicillin-resistant Staphylococcus (MRS) bacteria, including methicillin-resistant S. aureus and methicillin-resistant S. pseudintermedius (MRSP), pose a significant threat in veterinary medicine, given their potential for zoonotic transmission and their implications for companion animals and humans' health. This study aimed to assess the prevalence of MRS and anti-microbial resistance patterns at a university clinical hospital in Madrid, Spain. Samples were collected from both the environment and hospital staff at Veterinary Clinical Hospital of Alfonso X el Sabio University. Anti-microbial susceptibility assays, molecular detection of mecA gene and genetic relationships among the identified bacterial strains were performed. The study revealed an MRS prevalence of 1.50% in environmental samples, with MRSP accounting for 0.75% of the cases. Genetically related MRSP strains were found in different hospital areas. Among hospital staff, there was a MRS prevalence of 14.03%, including S. pseudintermedius and S. epidermidis strains. Antibiogram tests revealed multi-drug resistance among MRSP strains. Additionally, methicillin-resistant coagulase-negative staphylococci were isolated, suggesting potential cross-species transmission. This study underscores the presence of MRS in a veterinary clinical hospital, highlighting the significance of infection control through the implementation of protective measures, stringent hygiene practices among personnel and in the environment and responsible use of antibiotics. Further research is necessary to assess MRS incidence in animal patients and explore geographical variations, enhancing our understanding of MRS in veterinary medicine and addressing its challenges.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"325-334"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran. 用分离、血清学和分子方法筛查伊朗东阿塞拜疆省的马勒氏伯克霍尔德氏菌。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2024.2010651.4002

Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi

{"title":"Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran.","authors":"Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi","doi":"10.30466/vrf.2024.2010651.4002","DOIUrl":"10.30466/vrf.2024.2010651.4002","url":null,"abstract":"Glanders caused by Burkholderia mallei is one of the most dangerous zoonotic diseases in solipeds. Clinical diagnosis of this disease in its early stages in horses, is difficult. This study investigated serological and molecular identification of B. mallei in East Azerbaijan province. In the third and fourth quarters of 2020, throughout 2021, and in the first and second quarters of 2022, the complement fixation test (CFT) was performed on 350 horses. The malleination was used to confirm the positive CFT cases. Blood samples were taken for culture and for preparing serums to perform the enzyme-linked immunosorbent assay (ELISA). Deep eye discharge, nostril, cutaneous ulcers and lymph fluid swabs were cultured, and polymerase chain reaction (PCR) was carried out. Eleven horses were CFT-positive. Based on the malleination on the 11 horses, six were affected by glanders, five were not affected (false positive), and one horse was CFT-negative despite exhibiting clinical signs. It was positive by malleination, ELISA and PCR. A total number of seven positive cases of glanders were diagnosed. The B. mallei could not be isolated, but the Burkholderia cepacia complex was isolated in one case. Except for three cases, the results of the CFT, mallein and ELISA tests were consistent. The amount of confidence interval was 95.00%. It is suggested that ELISA could be used as a complement to CFT in screening and, if positive results are observed in one of the tests, the entire herd must be examined more accurately using the mallein and western blot confirmatory tests.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"231-236"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt. 从埃及驼奶中分离出的金黄色葡萄球菌抗菌药耐药性基因的分子特征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2023.2007392.3946

Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey

{"title":"Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt.","authors":"Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey","doi":"10.30466/vrf.2023.2007392.3946","DOIUrl":"10.30466/vrf.2023.2007392.3946","url":null,"abstract":"Staphylococcus aureus is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of S. aureus in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for S. aureus using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of S. aureus isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for S. aureus. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (mecA), β-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDRS aureus as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant S. aureus isolates.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"267-274"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice. 比较某些氨基糖苷类抗生素对小鼠运动行为的影响

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2001972.3891

Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin

{"title":"Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice.","authors":"Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin","doi":"10.30466/vrf.2023.2001972.3891","DOIUrl":"10.30466/vrf.2023.2001972.3891","url":null,"abstract":"Aminoglycoside antibiotics (AGs) can cause neuromuscular blockade and paralysis of skeletal muscles. To compare the paralytic effects of selected AGs on some motor behaviors in mice, 24 male mice were divided into four groups. Each group was given one of AGs (gentamicin, dihydro-streptomycin, apramycin and amikacin) at incremental doses that increased half-logarithmically compared to the therapeutic dose (16.00 mg kg-1). Motor behavioral tests included open field test, inclined plane, horizontal bars, static rods, parallel bars and rotarod. Finally, the data were analyzed using descriptive and analytical statistics. Gentamicin and dihydrostreptomycin at 32.00 times of the therapeutic dose produced complete paralysis of the limbs, respiratory arrest, and even death in some animals. However, apramycin and amikacin did not show significant effects on skeletal muscle and motor behaviors at 32.00 times of the therapeutic dose. After administration of apramycin at 100 times of the therapeutic dose, four out of six mice (66.67%) died from respiratory depression. Amikacin at this dose did not cause animal death, although it caused some changes in motor behaviors with a significant difference in comparison with control values. Gentamicin demonstrated significantly more potent effects on motor behaviors compared to the other AGs. Overall, the order of potency was gentamicin > dihydrostreptomycin > apramycin > amikacin. High doses of AGs could impair the skeletal muscle function and disrupt motor behaviors in mice. Furthermore, the paralytic potency of selected AGs on skeletal muscle was significantly different.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"97-104"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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