Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-07-15DOI: 10.30466/vrf.2024.2019729.4126
Bahareh Hafezi, Hossein Kazemi Mehrjerdi, Amir Moghaddam Jafari
{"title":"Effect of captopril on paraplegia caused by spinal cord ischemia-reperfusion injury in rats.","authors":"Bahareh Hafezi, Hossein Kazemi Mehrjerdi, Amir Moghaddam Jafari","doi":"10.30466/vrf.2024.2019729.4126","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019729.4126","url":null,"abstract":"<p><p>This study investigated the effect of captopril (Cap) on spinal cord ischemia-reperfusion injury (SCII) in rats. Twenty-four adults male Wistar rats were randomly divided into four groups of six animals each: spinal cord ischemia-reperfusion (SCI-R) with Cap (SCI-R + Cap), SCI-R, sham-operated with Cap (SHAM + Cap), and SHAM. The 24 hr and 90 min before ischemia induction, Cap was administered intragastrically (100 mg kg<sup>-1</sup>) to the SHAM + Cap and SCI-R + Cap groups. Abdominal aortic clamping was performed in the SCI-R and SCI-R + Cap groups for 40 min. Hindlimb motor function was evaluated using the Tarlov Scale at 4, 6, 12, 24, 48, and 60 hr after SCII. The malondialdehyde (MDA), the ferric-reducing ability of plasma (FRAP) and prooxidant-antioxidant balance (PAB) values were also measured. Throughout the study period, the SCI-R group had significantly lower motor function scores compared to the other groups. The MDA and PAB levels were higher and the FRAP value was lower in the SCI-R group compared to in the SHAM group. The SCI-R + Cap had higher motor function scores compared to the SCI-R group at all time points. There were no significant differences in MDA concentration, FRAP and PAB values between the SCI-R + Cap and SCI-R groups. Captopril may act as a protective agent against SCII in rats based on hind limb motor function assessment.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"379-385"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-09-15DOI: 10.30466/vrf.2024.2013685.4035
Dana Dadras, Ahmad Imani, Naser Agh, Rasoul Shahrooz, Parisa Mohammadisefat
{"title":"Investigating the effects of different DHA/EPA ratios in rainbow trout (<i>Oncorhynchus mykiss</i>) egg composition on foregut development of larvae.","authors":"Dana Dadras, Ahmad Imani, Naser Agh, Rasoul Shahrooz, Parisa Mohammadisefat","doi":"10.30466/vrf.2024.2013685.4035","DOIUrl":"10.30466/vrf.2024.2013685.4035","url":null,"abstract":"<p><p>Lecithotrophic larvae utilize extensive yolk reserves for early development. In this study, the effect of egg docosahexaenoic acid (DHA):eicosapentaenoic acid (EPA) ratios (<i>i.e.</i>, 5.92, 10.08, 11.66, and 14.53) on the emerging larvae foregut development of rainbow trout was examined. Larvae samples were taken from day 22 to 36 post-fertilization. Thin whole body longitudinal sections were prepared and stained by Hematoxylin and Eosin and Alcian blue procedure. The sections were examined regarding epithelial layer thickness, intestinal fold height and mucosal layer thickness along with number of enterocytes and goblet cells. Results indicated that maximum thickness of the epithelium was observed on day 36 post fertilization in larvae hatched from eggs with DHA:EPA ratios of 14.53 and 10.08. The highest and lowest intestinal folds height were also observed in larvae hatched from eggs with DHA:EPA ratios of 10.08 and 14.53, respectively. The mucosal-submucosa layer thickness was the highest in larvae hatched from eggs with DHA:EPA ratio of 10.08. Enterocyte's count was the highest in larvae obtained from eggs with DHA:EPA ratio of 10.08 on day 36 post-fertilization. The highest and lowest number of goblet cells were enumerated in larvae obtained from eggs with DHA:EPA ratios of 5.53 and 14.53, respectively. In conclusion, our results revealed that feeding rainbow trout broodstock with diet contained highly unsaturated fatty acids (HUFA):polyunsaturated fatty acids (PUFA) ratio of 0.28 could result in the egg with DHA:EPA ratio of 10.08 which in turn yielded larvae with better foregut development parameters compared to those larvae emerged from the eggs with increased DHA :EPA ratio.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"463-471"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-10-15DOI: 10.30466/vrf.2024.2023763.4188
Alekhin Yuri Nikolaevich, Popova Olga Sergeevna, Ponamarev Vladimir Sergeevich
{"title":"Features of glucose-insulin homeostasis at different stages of gestation in cows.","authors":"Alekhin Yuri Nikolaevich, Popova Olga Sergeevna, Ponamarev Vladimir Sergeevich","doi":"10.30466/vrf.2024.2023763.4188","DOIUrl":"10.30466/vrf.2024.2023763.4188","url":null,"abstract":"<p><p>An increase in morbidity during times of relatively high functional load on the body such as during pregnancy, confirms the role of metabolic overload in the occurrence of metabolic failures. It is better to take preventive measures such as adjusting metabolic regulation mechanisms in light of the ideal dietary composition. However, this direction is constrained by the lack of information about neurohumoral regulation. The goal of the present study was to learn more about the dynamics of changes in insulin and glucose levels in pregnant cow's blood. Research on the levels of glucose and insulin in lactating cows demonstrated that ruminants had distinct characteristics in the metabolism of carbohydrates, most notably a lessened reliance of blood glucose on insulin levels. A rise in blood glucose and insulin levels was observed as the gestational stage increased during the third trimester of pregnancy. The intensity of this insulin tolerance was contingent upon the level of productivity and glucose levels during the preceding stages of gestation.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"523-528"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-10-15DOI: 10.30466/vrf.2024.2025342.4218
Ensiye Sajadian Jaghargh, Mohammad Taghi Vajed Ebrahimi, Armin Attaranzadeh, Abbas Parham, Ahmad Reza Mohammadnia
{"title":"Histopathological evaluation of the viability of auto-transplanted ovary following vitrification-thawing process in rabbit as an animal model.","authors":"Ensiye Sajadian Jaghargh, Mohammad Taghi Vajed Ebrahimi, Armin Attaranzadeh, Abbas Parham, Ahmad Reza Mohammadnia","doi":"10.30466/vrf.2024.2025342.4218","DOIUrl":"10.30466/vrf.2024.2025342.4218","url":null,"abstract":"<p><p>Cryopreservation and re-transplantation of ovarian tissue is a relatively new technique to preserve fertility. This study evaluated the preservation of ovarian follicles after the vitrification-thawing procedure by implanting ovarian fragments into the gluteus muscle of rabbit. Bilateral ovariectomy was performed on each rabbit. The cortices were separated from the medullae of ovaries and divided into four fragments. One fresh cortical fragment was used as a control, fixed in 10.00% formalin. Three fragments underwent vitrification-thawing for two weeks. Two of these vitrified-thawed fragments were auto-transplanted into separate locations within the opened gluteus muscle, while the final fragment was fixed in 10.00% formalin. Eight weeks after re-implantation, biopsies were collected from the ovarian fragments and fixed in 10.00% formalin. The numbers and areas (µm<sup>2</sup>) of morphologically normal follicles were evaluated on sections stained with Hematoxylin and Eosin through light microscopy. The follicular morphology in the ovarian fragments immediately after vitrification-thawing remained similar to that of the fresh ovary. The number of morphologically intact follicles was significantly lower in the ovarian fragments eight weeks after re-implantation than fresh ovary and ovarian tissue fragments after vitrification-thawing procedure. However, follicular development to the antral stage was observed in all samples eight weeks after re-implantation. There was no statistical difference in the areas (µm<sup>2</sup>) of primordial, primary, pre-antral, and antral follicles in the ovarian fragments before cryopreservation, immediately after vitrification-thawing, and following re-implantation procedures. Auto-transplantation of ovarian tissue into the gluteus muscle of rabbits could be a viable approach for preserving ovarian follicles after vitrification-thawing procedure.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"545-550"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-10-15DOI: 10.30466/vrf.2024.2017700.4099
Mehdi Gharakhani, Mohammad Faezi Ghasemi, Pejvak Khaki, Majid Esmaelizad, Majid Tebianian
{"title":"Immunological characterization of recombinant outer membrane Loa22 protein of local pathogenic <i>Leptospira</i> serovars.","authors":"Mehdi Gharakhani, Mohammad Faezi Ghasemi, Pejvak Khaki, Majid Esmaelizad, Majid Tebianian","doi":"10.30466/vrf.2024.2017700.4099","DOIUrl":"10.30466/vrf.2024.2017700.4099","url":null,"abstract":"<p><p>Leptospirosis is a worldwide zoonotic disease caused by pathogenic <i>Leptospira</i> spp<i>.</i>, often occurring in tropical and subtropical regions. Focusing on development of rapid diagnostic methods to facilitate early diagnosis and a universal vaccine are the main critical issues to overcome the burden of leptospirosis. Here, we have studied the immunogenic potential of prepared recombinant Loa22 protein (rLoa22) of local pathogenic <i>Leptospira</i> species in mice and its ability to induce humoral and cellular immunity, being further evaluated by analyzing the immunoglobulin G (IgG) subclasses and cytokines produced through immunization. Based on the results, mice immunized with rLoa22/adjuvant and a trivalent vaccine, induced high titers of total IgG. All immunized groups increased IgG1 almost on the same level; but, IgG2a level was significantly higher in the vaccine and rLoa22/adjuvant groups than rLoa22 alone group. Animals immunized with the vaccine produced more interleukin 4 than rLoa22/ adjuvant group. The results of evaluating interferon gamma level showed that the rLoa22/adjuvant and vaccine groups had a significant increase compared to the rLoa22 alone group. The results also demonstrated that the rLoa22 protein, in indirect enzyme-linked immunosorbent assay, was able to detect the anti-<i>Leptospira</i> antibodies in mice serum that can be used as a marker in assessing the seroprevalence of leptospirosis and/or in combination with other leptospiral antigens in development of an effective vaccine against leptospirosis.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"565-573"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-10-15DOI: 10.30466/vrf.2024.2024103.4193
Nurten Yilmaz
{"title":"Quantitative analysis of biogenic amine production of different lactic acid bacteria isolated from ready-to-eat packaged fish products.","authors":"Nurten Yilmaz","doi":"10.30466/vrf.2024.2024103.4193","DOIUrl":"10.30466/vrf.2024.2024103.4193","url":null,"abstract":"<p><p>The ability of lactic acid bacteria (LAB) to produce biogenic amines (BAs) from amino acids using amino acid decarboxylase enzymes is an important food safety criterion due to their use as starter or bio-protective cultures. In this study, various LAB isolates, including <i>Lactococcus</i> (three isolates), <i>Lactobacillus</i> (five isolates), <i>Enterococcus</i> (13 isolates), and <i>Leuconostoc</i> (seven isolates), were isolated from ready-to-eat fish products like sauced, marinated, smoked anchovies, and smoked mackerel. These isolates were then tested for their ability to produce BAs (histamine, putrescine, cadaverine, spermidine, tryptamine, 2-phenylethylamine, spermine, serotonin, tyramine, 3-methylamine, and dopamine) and ammonia in histidine decarboxylase broth. The post-biotic solutions (cell-free supernatant) of <i>Lb. paracasei</i> NZ.Lbp.111, <i>Lb. brevis</i> NZ.Lbb.085, and <i>E. casseliflavus</i> NZ.Ec.074 exhibited the highest ammonia levels. Histamine accumulation was generally low in post-biotic solutions from four LAB isolates, ranging from 2.00 to 7.78 ppm. However, the post-biotic solution of <i>Leu. mesenteroides</i> NZLeu 009 displayed a significantly higher histamine concentration (83.23 ppm). <i>Lactobacillus</i> post-biotic solutions had the highest concentrations of ammonia (1220.28 ppm), 2-phenylethylamine (82.96 ppm), serotonin (278.70 ppm), tyramine (267.48 ppm), and cadaverine (19.72 ppm). Also, the average BAs concentration for <i>Lactobacillu</i>s post-biotic solutions was the highest at 31.98 ppm. The results revealed the analysis of BAs concentrations in different LAB isolates from fish products, providing interesting insights into their metabolic capabilities and potential implications for food safety and quality.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"537-543"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2023.2009350.3978
Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili
{"title":"Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens.","authors":"Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili","doi":"10.30466/vrf.2023.2009350.3978","DOIUrl":"10.30466/vrf.2023.2009350.3978","url":null,"abstract":"<p><p>Purification is an important step in the production of viral vaccines that strongly affects product recovery and subsequent immune responses. The present study was carried out with the aim of improving the purification of infectious bursal disease virus (IBDV) by the tangential flow filtration (TFF) method. Then, the effect of the purified virus on the induction of immune responses against IBDV in specific pathogen free (SPF) chickens was investigated. The IBD07IR strain was propagated in embryonated SPF eggs. The virus was purified using a 100 kDa cassette. The quality of the recovered viruses was evaluated by titration. A total number of 60 SPF chickens were randomly divided into three groups (n = 20) and received the concentrated viral antigen, commercial live IBDV vaccine and phosphate-buffered saline at the age of 3 weeks by eye drop method. The bursa of Fabricius was examined histopathologically for possible changes. Sera were collected at 1-week intervals from day 0 until the end of 6 weeks after vaccination. The IBDV-specific antibody levels, induction of cell-mediated immunity and mRNA expression levels of cytokines were evaluated. The results showed that despite a relative raise in virus titer from 7.66 to 8.17 embryo infectious dose (EID)<sub>50</sub> mL<sup>-1</sup> following purification, both the purified IBDV and commercial vaccine are able to induce strong immune responses against the virus. Within a context of egg-based IBDV vaccine production, a single-step TFF can be applied for the relatively purification. This platform requires a further study in the selection of multiple membranes to optimize the operating conditions and final product.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"49-55"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cell proliferation and apoptosis in canine oral papillomatosis.","authors":"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz","doi":"10.30466/vrf.2023.1996086.3818","DOIUrl":"10.30466/vrf.2023.1996086.3818","url":null,"abstract":"<p><p>This study was aimed at the evaluation of cell proliferation, <i>p53</i> level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant <i>p53</i> gene. Despite the mutation of <i>p53</i> gene, the shift in the <i>Bax</i>/<i>Bcl-2</i> ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic <i>Bax</i> gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"75-82"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expression, purification and immunogenicity analyses of receptor binding domain protein of severe acute respiratory syndrome coronavirus 2 from delta variant.","authors":"Liqing Liu, Hong Chen, Ping Liu, Xiongfeng Ke, Jingjing Song, Ying Fang, Lingbao Kong, Xiu Xin","doi":"10.30466/vrf.2024.2013858.4037","DOIUrl":"10.30466/vrf.2024.2013858.4037","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. The receptor binding domain (RBD), located at the spike protein of SARS-CoV-2, contains most of the neutralizing epitopes during viral infection and is an ideal antigen for vaccine development. In this study, bioinformatic analysis of the amino acid sequence data of SARS-CoV-2 RBD protein for the better understanding of molecular characteristics was performed. The <i>SARS-CoV-2 RBD</i> gene was inserted into pET-28a vector, and efficiently expressed in <i>E. coli</i> system. Then, the recombinant proteins (RBD monomer and RBD dimer protein) were purified as antigen for animal immunization. Furthermore, the results showed that the recombinant proteins (RBD monomer and RBD dimer protein) had adequate immunogenicity to stimulate specific antibodies against the corresponding protein in immunized mice. Taken together, the results of this study revealed that RBD protein had a high immuno-genicity. This study might have implications for future development of SARS-CoV-2 detection.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"657-663"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-05-15DOI: 10.30466/vrf.2024.2004618.3913
Muhammad Awais Ajmal, Mubbashar Hassan, Muhammad Shahzad, Muhammad Ilyas Naveed, Aijaz Ali Channa, Nasim Ahmad
{"title":"Follicular, luteal dynamics and effect of synchronization methods on reproductive responses in Cholistani cows.","authors":"Muhammad Awais Ajmal, Mubbashar Hassan, Muhammad Shahzad, Muhammad Ilyas Naveed, Aijaz Ali Channa, Nasim Ahmad","doi":"10.30466/vrf.2024.2004618.3913","DOIUrl":"10.30466/vrf.2024.2004618.3913","url":null,"abstract":"<p><p>The Cholistani cow is considered one of the milch breeds of Zebu cattle. Despite being heat and tick-resistant, it has not yet gained much popularity among Pakistan dairy farmers due to its long calving interval. This study aimed to understand the basic reproductive physiology of Cholistani cows using sonography and reproductive biotechnologies such as estrus synchronization and timed artificial insemination to improve reproductive efficiency. In experiment 1, six Cholistani cows with mixed parity 3.20 ± 1.30 and weighing 400 kg were selected to monitor ovarian dynamics on alternate days by the same sonographer from the onset of heat through ovulation until the next ovulation. Experiment 2 measured the effect of estrus synchronization methods, controlled internal drug release-gonadotropin-releasing hormone (CIDR-GnRH, n = 31) and Ovsynch (OVS, n = 32) on various reproductive parameters. The mean estrous cycle length was 19.81 ± 0.56 days with two follicular waves. The mean inter-ovulatory interval was 20.80 ± 0.52 days, with a preovulatory follicular size of 13.83 ± 2.37 mm. Estrus response was higher (<i>p</i> > 0.05) in controlled internal drug release (CIDR)-GnRH (93.54%) than in OVS (84.37%) cows. Similarly, ovulation and conception rates were higher in CIDR-GnRH (91.66% <i>vs</i>. 68.42%) than in OVS cows (41.37% <i>vs.</i> 33.33%), respectively. In conclusion, CIDR-GnRH results in a better estrus response, higher ovulation rate, and subsequently greater conception rate than OVS in <i>Bos indicus</i> dairy cows.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"251-255"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}