Veterinary Research Forum最新文献_第9页

Evaluation of the relationship between inflammatory reaction and interleukins in ovine pulmonary adenocarcinomas. 羊肺腺癌炎症反应与白细胞介素关系的评价。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.542311.3279

Emin Karakurt, Nüvit Coşkun, Enver Beytut, Ömer Faruk Keleş, Serpil Dağ, Volkan Yılmaz, Hilmi Nuhoğlu, Ayfer Yıldız, Emre Kurtbaş

{"title":"Evaluation of the relationship between inflammatory reaction and interleukins in ovine pulmonary adenocarcinomas.","authors":"Emin Karakurt, Nüvit Coşkun, Enver Beytut, Ömer Faruk Keleş, Serpil Dağ, Volkan Yılmaz, Hilmi Nuhoğlu, Ayfer Yıldız, Emre Kurtbaş","doi":"10.30466/vrf.2022.542311.3279","DOIUrl":"https://doi.org/10.30466/vrf.2022.542311.3279","url":null,"abstract":"In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 - 2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 - 2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor-associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/d1/vrf-14-1.PMC9906612.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of canine distemper in dogs referred to Veterinary Hospital of Ferdowsi University of Mashhad, Mashhad, Iran. 伊朗马什哈德费尔多西大学兽医医院犬犬瘟热流行情况。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.541661.3269

Farzad Mousafarkhani, Ali Asghar Sarchahi, Hadi Mohebalian, Javad Khoshnegah, Mohammad Arbabi

{"title":"Prevalence of canine distemper in dogs referred to Veterinary Hospital of Ferdowsi University of Mashhad, Mashhad, Iran.","authors":"Farzad Mousafarkhani, Ali Asghar Sarchahi, Hadi Mohebalian, Javad Khoshnegah, Mohammad Arbabi","doi":"10.30466/vrf.2022.541661.3269","DOIUrl":"https://doi.org/10.30466/vrf.2022.541661.3269","url":null,"abstract":"Canine distemper virus (CDV) is responsible for high morbidity and mortality in dogs worldwide. Epidemiological study of canine distemper can help to control and treat the disease in any area. This study aimed to investigate the prevalence of CDV in dogs referred to the Veterinary Hospital from September 23, 2018 to September 22, 2019. Dogs with at least two clinical signs of canine distemper underwent blood tests, rapid test kit from the eye and cerebrospinal fluid (CSF), and RT-PCR from whole blood and/or CSF samples. Out of 1212 referred dogs, 112 dogs were suspected to have canine distemper of which 90 underwent RT-PCR and rapid test kits. The disease prevalence was 4.04% (49/1212) and 7.44% (49/659) according to the total number of referring dogs and number of referring sick dogs, respectively. The distemper fatality rate was 69.57% (32/46). Seventy percent of distemper positive cases were under 12 months old and 52.08% were under 6 months old. Female dogs were more susceptible than males; however, the fatality rate of males was more than females. Of distemper positive dogs, 91.84% were unvaccinated. The highest prevalence (71.43%) of dogs diagnosed with CDV occurred during the cold seasons. It is concluded that canine distemper is endemic in the geographical area of Mashhad and its prevalence rate in dogs referred to the Veterinary Hospital of Ferdowsi University of Mashhad is 4.04% and its fatality rate is 69.57%. This indicates that a significant number of dogs may die if they develop distemper despite treatment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/db/vrf-14-153.PMC10073810.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9326065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of adenosine N1-Oxide and pioglitazone on inflammatory and antioxidant state in sepsis caused by experimental cecal puncture in rat. 腺苷n1 -氧化物和吡格列酮对实验性盲肠穿刺脓毒症大鼠炎症和抗氧化状态的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.562229.3625

Yaser Jafari-Khataylou, Siamak Kazemi-Darabadi, Somayeh Ahmadi Afshar

{"title":"Effects of adenosine N1-Oxide and pioglitazone on inflammatory and antioxidant state in sepsis caused by experimental cecal puncture in rat.","authors":"Yaser Jafari-Khataylou, Siamak Kazemi-Darabadi, Somayeh Ahmadi Afshar","doi":"10.30466/vrf.2022.562229.3625","DOIUrl":"https://doi.org/10.30466/vrf.2022.562229.3625","url":null,"abstract":"Sepsis is an acute condition caused by the systemic inflammatory response syndrome to an infection. There are very few drugs that could improve the severe conditions in patients with sepsis. Hence, it is important to consider different treatment options. In this survey, we studied the effect of adenosine N1-oxide (ANO) and pioglitazone on rats with cecal ligation and perforation (CLP). They were randomly divided to four groups (n = 10) including Group A: as control group receiving normal saline, Group B: the rats with CLP as surgical control group, Group C: the rats receiving ANO, and Group D: the rats receiving pioglitazone. Interleukin (IL) -6, IL-1β, tumor necrosis factor alpha (TNF-α), nitric-oxide (NO) in serum blood and superoxide dismutase (SOD), catalase (CAT) malondialdehyde, (MDA) and myeloperoxidase (MPO) in liver and spleen homogenates were measured. The amount of antioxidant enzymes in the spleen and liver, and finally cell viability and rats' survival were investigated. The measurement of blood serum nitric-oxide and survival of all groups of rats were also performed. The results indicated that both drugs could cause a decrease in IL-1β, IL-6, TNF-α and NO in rat blood serum and MDA and MPO in the liver and spleen homogenates, however, a significant increase in SOD and CAT in the liver and spleen homogenates in rats that received ANO and pioglitazone was observed compared to rats with CLP group. Cell viability and rats' survival were significantly improved in rats that received ANO and pioglitazone compared to rats with CLP group. Adenosine N1-oxide showed stronger and more effective effects.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/60/vrf-14-381.PMC10410109.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of pyrethroids resistance alleles in goat biting louse Bovicola caprae (Phthiraptera: Trichodectidae) in west and northwest of Iran. 伊朗西部和西北部山羊咬虱Bovicola caprae（Phthiraptera:毛滴虫科）中拟除虫菊酯类抗性等位基因的检测。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-09-15 DOI: 10.30466/vrf.2023.556815.3565

Khadijeh Seydi Gazafi, Mousa Tavassoli, Karim Mardani

{"title":"Detection of pyrethroids resistance alleles in goat biting louse Bovicola caprae (Phthiraptera: Trichodectidae) in west and northwest of Iran.","authors":"Khadijeh Seydi Gazafi, Mousa Tavassoli, Karim Mardani","doi":"10.30466/vrf.2023.556815.3565","DOIUrl":"10.30466/vrf.2023.556815.3565","url":null,"abstract":"Pyrethroid insecticides target voltage-gated sodium channels (VGSCs) that are essential for electrical signaling in the nervous system of insects. Three-point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F located in domain II conferring the knockdown resistance (kdr) are the most important mutations in pyrethroid-resistant lice worldwide. In addition, six new mutations have been reported in the extracellular loops IIS1-2 (H813P) and IIS5 (I927F, L928A, R929V, L930M, L932M) in the α- subunit of the sodium channel in lice. The aim of this study was to detect alleles resistant to pyrethroids in the domain II (S5-S6) of the VGSC gene in goat biting louse. Goat biting lice were collected from five provinces in the west and northwest of Iran. Genomic DNA was extracted from goat biting lice and Bovicola (Damalinia) caprae species was confirmed by amplifying the cytochrome oxidase subunit I (COXI) gene. A fragment in the domain II (S5-S6) of the VGSC gene was amplified using the specific primers and the resultant polymerase chain reaction products were sequenced. Substitutions T917I, L920F, I927F, L928A, R929V and L930M were identified in the examined sequences. The results showed that all the examined lice had at least one mutation in their VGSC gene associated with pyrethroid resistance or new mutations. The presence of these mutated alleles in the VGSC gene may be due to the long-term and multiple use of pyrethroids against arthropods. Thus, the molecular detection of resistance to pyrethroid insecticides in goat chewing lice can help plot a kdr frequency map to enact effective policies to control caprine pediculosis.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/fb/vrf-14-489.PMC10560324.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How origin of ovaries influences the vitrification outcome of bovine ovarian tissue: effects of side of ovaries and corpus luteum. 卵巢来源如何影响牛卵巢组织的玻璃化结果：卵巢一侧和黄体的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-12-15 DOI: 10.30466/vrf.2023.1972637.3684

Mohammad Hamed Shahsavari, Gholamali Moghaddam, Kele Amaral Alves, Benner Geraldo Alves, Laritza Ferreira de Lima, Golshan Azimi, Deysi Juana Dipaz Berrocal, Luciana Mascena Silva, Yago Pinto da Silva, Diego Alberto Montano Vizcarra, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues

{"title":"How origin of ovaries influences the vitrification outcome of bovine ovarian tissue: effects of side of ovaries and corpus luteum.","authors":"Mohammad Hamed Shahsavari, Gholamali Moghaddam, Kele Amaral Alves, Benner Geraldo Alves, Laritza Ferreira de Lima, Golshan Azimi, Deysi Juana Dipaz Berrocal, Luciana Mascena Silva, Yago Pinto da Silva, Diego Alberto Montano Vizcarra, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues","doi":"10.30466/vrf.2023.1972637.3684","DOIUrl":"10.30466/vrf.2023.1972637.3684","url":null,"abstract":"Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL- (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) In vitro culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries). The current study shows that the CL- and right groups had the greatest percentage of follicles with normal morphology compared to other vitrified-warmed groups. Furthermore, the levels of necrosis and tissue damage of the right cultured group were the lowest compared to other groups. It was shown that bovine ovarian tissues derived from right ovaries and ovaries without a corpus luteum can be functionally and morphologically preserved after vitrification. For the first time, the present study suggests that bovine ovarian tissue vitrification can be improved by considering the origin of the ovaries.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ozone therapy on acidic corneal burns in rats. 臭氧治疗对大鼠酸性角膜烧伤的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.551199.3432

Seren Aydoğan, Hanifi Erol, Münevver Baran

{"title":"Effects of ozone therapy on acidic corneal burns in rats.","authors":"Seren Aydoğan, Hanifi Erol, Münevver Baran","doi":"10.30466/vrf.2022.551199.3432","DOIUrl":"https://doi.org/10.30466/vrf.2022.551199.3432","url":null,"abstract":"In the present study the effect of ozone therapy on hydrofluoric acid (HFA) related eye burn was investigated in rats. A Total 20 healthy male Wistar albino rats (weighing 250 - 300 g with the age of 16 weeks) were used. They were divided into groups (experimental and control groups) of 10 rats being housed individually and fed ad libitum. The HFA (2.00%) burn was created in all animals. The ozonized (20.00 µg O3 mL-1) bi-distilled water was applied as a drop (10.00 µL each drop) every 8 hr for 7 days in the experimental group. At the same time, 0.90% NaCl was applied as drop (10.00 µL each drop) every 8 hr for 7 days in the control group. In the experimental group, intensive inflammation, angiogenesis, epithelial damage and stromal edema were detected in one animal. Epithelial vascularization and stromal edema were seen in four animals. In control group, only two animals' corneal structures were normal. Inflammation, angiogenesis, epithelial damage, fibrosis, epithelial vascularization and stromal edema were detected in the rest. As a result of this study, it was observed that local usage of ozone therapy had a positive effect on the healing of corneal burns caused by HFA. It was concluded that more ozone-related studies should be done to enlighten the subject.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/52/54/vrf-14-195.PMC10170467.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Phylogenetic analysis of pigeon adenovirus 1 in clinical specimens of domestic pigeons (Columba livia domestica) in Iran. 伊朗家鸽临床标本中鸽子腺病毒1型的系统发育分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.553719.3471

Elham Rahimi Sardo, Forough Talazadeh, Ramezan Ali Jafari, Masoud Reza Seifi

{"title":"Phylogenetic analysis of pigeon adenovirus 1 in clinical specimens of domestic pigeons (Columba livia domestica) in Iran.","authors":"Elham Rahimi Sardo, Forough Talazadeh, Ramezan Ali Jafari, Masoud Reza Seifi","doi":"10.30466/vrf.2022.553719.3471","DOIUrl":"https://doi.org/10.30466/vrf.2022.553719.3471","url":null,"abstract":"An internationally identified syndrome that leads to deaths between domestic and ornamental pigeons, particularly after racing is young pigeon disease syndrome (YPDS). This study was conducted to determine the status of pigeon adenoviral infection and molecularly characterize the pigeon adenovirus in Ahvaz pigeons. Sixty stool samples of healthy pigeons (young pigeons and adult pigeons) and 60 stool samples of diseased pigeons (young and adults) with symptoms of lethargy, weight loss, crop stasis, vomiting and diarrhea were examined. Samples were screened for aviadenoviruses by polymerase chain reaction (PCR) assay and degenerated primers set to target the aviadenovirus polymerase (pol) gene were used which was designed in this study. Screening for pigeon adenovirus 1 (PiAdV-1) was performed using a primer pair that targeted the fiber gene of PiAdV-1. Out of 120 stool samples, six samples (5.00%) were positive for aviadenovirus. The results showed that independent from pigeons' age status, 5.00 and 3.33% of sick and of healthy pigeons were positive for PiAdV-1, respectively. Genomic sequencing revealed that the viruses detected in Ahvaz pigeons belonged to the PiAdV-1 genotype. The results in pigeons revealed a 98.10 - 99.53% nucleotide similarity when compared to other strains of PiAdV-1 (TR/SKPA20, P18-05523-6 and strain IDA4) formerly deposited in GenBank® in Türkiye, Australia and The Netherlands. As far as the authors know, this was the first record of phylogenetic analysis of PiAdV-1 in Iran.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/2c/vrf-14-329.PMC10298839.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9727511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic detection of Coxiellaburnetii based on plasmid genes in horses. 基于质粒基因的马科谢伯氏菌基因组检测。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.551663.3443

Manizheh Tehrani, Abdolghaffar Ownagh

{"title":"Genomic detection of Coxiellaburnetii based on plasmid genes in horses.","authors":"Manizheh Tehrani, Abdolghaffar Ownagh","doi":"10.30466/vrf.2022.551663.3443","DOIUrl":"https://doi.org/10.30466/vrf.2022.551663.3443","url":null,"abstract":"Q fever is a worldwide zoonosis caused by an obligate intra-cellular pathogen called Coxiella burnetii affecting a broad range of animal hosts including horses. Most of the isolates found carry plasmids which genetic studies of C. burnetii strains suggest a critical role in C. burnetii survival. The correlation between an isolated plasmid type and the chronic or acute nature of the disease has always been controversial. This study was conducted to investigate the prevalence of C. burnetii QpH1 and QpDG plasmids in horses and assess the potential role of these species as reservoirs of infection and transmission. Nested-polymerase chain reaction (PCR) assays were performed on 320 blood serum samples drawn from horses in West Azerbaijan province, Iran, in 2020. In total, 26 (8.13%) Q fever-positive samples based on containing the IS1111 gene were tested by nested-PCR approach to amplify QpH1 and QpDG plasmid segments. The QpH1 and QpRS plasmid-specific sequences were identified in 19 (73.07%) and none in the serum samples, respectively. According to the present study, the age of the animal can be considered as an important risk factor for the prevalence of C. burnetii; but, the season, sex, and breed of the horse had no effect on the prevalence of disease. The results indicate that nested-PCR method could be suitable for routine diagnosis, to gather new information about the shedding of C. burnetii, and to improve the knowledge of contamination routes.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/4d/vrf-14-317.PMC10298835.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9735905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of sperm characteristics and antioxidant and oxidant levels in bull semen frozen with four widely used extenders. 用四种常用膨化剂冷冻公牛精液的精子特性及抗氧化剂和氧化剂水平的比较。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2023.562594.3631

Kimia Maleki, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh

{"title":"Comparison of sperm characteristics and antioxidant and oxidant levels in bull semen frozen with four widely used extenders.","authors":"Kimia Maleki, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh","doi":"10.30466/vrf.2023.562594.3631","DOIUrl":"https://doi.org/10.30466/vrf.2023.562594.3631","url":null,"abstract":"Sperm survives for a very short time in fresh semen, and slow cooling to 5.00 ˚C kills a large number of sperms. This study was aimed to compare the semen quality parameters and anti-oxidant levels in four extenders (manual, Triladyl, Steridyl and AndroMed). Semen samples were obtained from a total number of 12 dual-purpose Simmental bulls kept in the Simmental Cattle Breeding Center for a period of 3 months using an artificial vagina. Sperm viability, motility, abnormal morphology, plasma membrane integrity, DNA damage, chromatin quality, total antioxidant capacity (TAC) and lipid peroxidation were evaluated. The highest progressive motility, viability, plasma membrane integrity, and TAC and the lowest levels of malondialdehyde in the frozen-thawed semen belonged to the semen group frozen with Triladyl. Parameters of motility were higher in the frozen-thawed semen with Triladyl than in other groups, indicating a significant difference from the manual extender. Among the extenders studied, Triladyl was the most suitable for semen freezing in Simmental bulls.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/84/vrf-14-373.PMC10410108.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Phylogenetic analysis of pathogenic Candida spp. in domestic pigeons. 家鸽致病性念珠菌的系统发育分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.555179.3499

Forough Talazadeh, Masoud Ghorbanpoor, Milad Masoudinezhad

{"title":"Phylogenetic analysis of pathogenic Candida spp. in domestic pigeons.","authors":"Forough Talazadeh, Masoud Ghorbanpoor, Milad Masoudinezhad","doi":"10.30466/vrf.2022.555179.3499","DOIUrl":"https://doi.org/10.30466/vrf.2022.555179.3499","url":null,"abstract":"The current study was conducted to survey the prevalence of pigeon candidiasis in diseased pigeons suspected to candidiasis by isolation, microscopic examination, and polymerase chain reaction (PCR) method and to characterize Candida spp. phylogenetically. For this purpose, samples were obtained from 100 suspected pigeons from September 2018 to February 2019 in Ahvaz, Iran. Cloacal and oropharyngeal swab samples were collected from each diseased pigeon with diarrhea resistant to the antibiotics, crop stasis, white diphtheritic membrane in the mouth, regurgitation, and vomiting. Sabouraud dextrose agar was used as a culture medium. Selected colonies were stained with lactophenol cotton blue stain. In the culture and direct microscopic observation, 19.00% of birds were suspected to candidiasis. Twenty-two isolates were identified. All 22 isolates were confirmed as Candida spp. By PCR method. The PCR test confirmed the presence of Candida spp. in 19.00% of pigeons. Based on the sequencing results of some PCR products, the isolates belonged to Candida albicans and Candida glabrata. The results revealed a 99.78% accordance when compared with other sequences of C. albicans which were formerly deposited in GenBank® from Colombia, Indonesia, China, and Sudan. The results revealed a 99.54% accordance when compared with other sequences of C. glabrata which were formerly deposited in GenBank® from the Netherlands and Spain. The symptoms such as diarrhea resistant to antibiotics, crop stasis, white diphtheritic membrane in the mouth, regurgitation, and vomiting were the most prevalent clinical symptoms in positive pigeons.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/68/32/vrf-14-431.PMC10475165.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10515161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1