Veterinary Research Forum最新文献_第9页

Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens. 超滤纯化传染性法氏囊病病毒对特定无病原体鸡免疫反应和细胞因子活化的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2009350.3978

Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili

{"title":"Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens.","authors":"Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili","doi":"10.30466/vrf.2023.2009350.3978","DOIUrl":"10.30466/vrf.2023.2009350.3978","url":null,"abstract":"Purification is an important step in the production of viral vaccines that strongly affects product recovery and subsequent immune responses. The present study was carried out with the aim of improving the purification of infectious bursal disease virus (IBDV) by the tangential flow filtration (TFF) method. Then, the effect of the purified virus on the induction of immune responses against IBDV in specific pathogen free (SPF) chickens was investigated. The IBD07IR strain was propagated in embryonated SPF eggs. The virus was purified using a 100 kDa cassette. The quality of the recovered viruses was evaluated by titration. A total number of 60 SPF chickens were randomly divided into three groups (n = 20) and received the concentrated viral antigen, commercial live IBDV vaccine and phosphate-buffered saline at the age of 3 weeks by eye drop method. The bursa of Fabricius was examined histopathologically for possible changes. Sera were collected at 1-week intervals from day 0 until the end of 6 weeks after vaccination. The IBDV-specific antibody levels, induction of cell-mediated immunity and mRNA expression levels of cytokines were evaluated. The results showed that despite a relative raise in virus titer from 7.66 to 8.17 embryo infectious dose (EID)50 mL-1 following purification, both the purified IBDV and commercial vaccine are able to induce strong immune responses against the virus. Within a context of egg-based IBDV vaccine production, a single-step TFF can be applied for the relatively purification. This platform requires a further study in the selection of multiple membranes to optimize the operating conditions and final product.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"49-55"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell proliferation and apoptosis in canine oral papillomatosis. 犬口腔乳头状瘤病的细胞增殖和凋亡。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.1996086.3818

Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz

{"title":"Cell proliferation and apoptosis in canine oral papillomatosis.","authors":"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz","doi":"10.30466/vrf.2023.1996086.3818","DOIUrl":"10.30466/vrf.2023.1996086.3818","url":null,"abstract":"This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"75-82"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tonsillar squamous cell carcinoma in a male Lhasa Apso. 一只雄性拉萨阿普索犬的扁桃体鳞状细胞癌。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2017462.4096

Alaleh Rakhshanpour, Sayyed Jafar Hasani, Ali-Asghar Tehrani, Mehdi Behfar

引用次数: 0

Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow. 揭示奶牛子宫纵隔肌瘤声像图和组织病理学的综合探索。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2018975.4112

Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar

{"title":"Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow.","authors":"Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar","doi":"10.30466/vrf.2024.2018975.4112","DOIUrl":"10.30466/vrf.2024.2018975.4112","url":null,"abstract":"Genital tumours are rare among cattle, largely due to their relatively short lifespans. Leio-myoma, a smooth muscle tumour being more prevalent in dogs, appears only at a rate of 1.00 - 2.00% in cattle, affecting reproductive efficiency in cases of complete uterine obstruction. This case report involves an 8-year-old cow with repeated insemination attempts unveiled 5.00 cm intra-luminal uterine mass, obstructing the right uterine horn. Transrectal sonography (TRUS) revealed a highly vascularized mass with normal ovarian function. Confirmation of clinical condition, i.e., uterine leiomyoma, via uterine biopsy concluded the presence of neoplastic smooth muscle cells arranged in interlacing bundles showing mild pleomorphism, and special staining using Masson's trichrome revealed an unappreciable amount of connective tissue; subsequently right flank celiotomy was performed to remove the benign tumour. Forty-five days after celiotomy, TRUS examination confirmed an unobstructed uterine horn, and bilateral oviduct patency was adjudged with 2.50% methylene blue. Following treatment for chronic endometritis, artificial insemination led to conception nearly 90 days post-procedure. The TRUS aids preliminary diagnosis, while definitive identification demands necropsy and surgical methods. This case underscores the diagnostic significance of TRUS, histopathology and celiotomy for identifying and managing uterine leiomyoma in cattle.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"207-210"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam. 氯胺酮、异丙酚和异氟烷对使用美托咪啶和咪达唑仑进行预处理的临床健康犬心电图变量的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2008055.3954

{"title":"Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam.","authors":"","doi":"10.30466/vrf.2024.2008055.3954","DOIUrl":"10.30466/vrf.2024.2008055.3954","url":null,"abstract":"The purpose of this study was to investigate the effects of three anesthetic agents, with premedication of medetomidine and midazolam, on electrocardiographic variables in dogs. Ten adult mixed breed dogs were used in a crossover design study, where they received ketamine, propofol and isoflurane treatments with a one-week washout period between them. In all three groups, medetomidine was administered first followed by midazolam after 15 min. Then, after 20 min, group 1 received ketamine intravenously (IV), group 2 received propofol (IV), and group 3 received isoflurane (inhalation). In all dogs, electrocardiographs were taken before and after premedication's, as well as every 15 min during anesthesia. Medetomidine significantly decreased heart rate and P wave amplitude and increased PR interval, R wave amplitude, QT interval, and T wave amplitude. Midazolam increased the amplitude of the R and T waves. Ketamine increased the heart rate and PR interval. Propofol increased the heart rate for up to 15 min, decreased the PR interval for up to 30 min, and the QT interval for up to 45 min. Isoflurane increased the heart rate and decreased the amplitude of R and T waves. The results showed that the drugs used in this study did not have many side effects on electrocardiographic variables and could be used without serious concern. The most important side effects observed were a severe reduction in heart rate and 1st degree atrioventricular (AV) block and, to a lesser extent, 2nd degree AV block caused by medetomidine and midazolam which were masked by the anesthetics.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"187-194"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test. 根据粪便幼虫数量减少试验评估阿苯达唑和左旋咪唑对羊群肺线虫的抗药性

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2023.2010062.3991

Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin

{"title":"Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test.","authors":"Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin","doi":"10.30466/vrf.2023.2010062.3991","DOIUrl":"10.30466/vrf.2023.2010062.3991","url":null,"abstract":"The over-use of anti-parasitic compounds as a method of control has led to insufficient effectiveness and widespread drug resistance worldwide. The aim of this study was to investigate the efficacy of albendazole and levamisole as anti-parasitic agents in a lung worm control program in goat flocks. During 2021 and 2022, a total of 110 goats (age of four months and above) were randomly selected from 11 herds in the north-western region of Iran including Saanen breed (both sexes of the same age). The results indicated that 3.60, 50.80 and 41.90% were respectively infected with Dictyocaulus filaria, Muellerius capillaris and Proto-strongylus rufescens, and generally all the lung parasites in goats of this region were resistant to albendazole and levamisole. Due to clinical importance of D. filaria in goats, the molecular analysis of two samples was also done. Sequencing results showed that the identified parasites were 100% similar to the reference sequences registered in the GenBank®. The results of this research showed low level of these anthelmintics efficacy against Dictyocaulus and Muellerius. Generally, the lung parasites in goats of this region are resistant to albendazole and levamisole. The P. rufescens showed high resistance to these drugs. Totally, it can be concluded that the level of drug resistance varies in different parts of the world; but, the frequencies of drug resistance in different parts of the world are not the same, requiring more studies.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"181-186"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and phylogenetic analysis of African swine fever virus from a pig farm in India. 印度某猪场非洲猪瘟病毒的分子特征和系统发育分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2022336.4158

Amit Kumar Verma, Vikas Jaiswal, Surendra Upadhyay, Arbind Singh, Afroz Afroz, Amit Kumar

{"title":"Molecular characterization and phylogenetic analysis of African swine fever virus from a pig farm in India.","authors":"Amit Kumar Verma, Vikas Jaiswal, Surendra Upadhyay, Arbind Singh, Afroz Afroz, Amit Kumar","doi":"10.30466/vrf.2024.2022336.4158","DOIUrl":"10.30466/vrf.2024.2022336.4158","url":null,"abstract":"African swine fever (ASF) is considered as one of the most threatening diseases for the pig farming industry all over the world. Due to the lack of an effective vaccine, organized farms and backyard rearing must strictly enforce control measures in order to combat the disease. The present report describes the ASF epidemic in a piggery in Uttar Pradesh state, India. The pathological samples were collected from the affected pigs and processed for histopathological and molecular studies. Gross lesions comprised of cyanosis of ear pinna, multi-focal hemorrhagic spots on ventral abdomen and inner aspect of thigh, highly congested mesenteric lymph nodes with marbling, marked congestion, hemorrhages and splenomegaly, interstitial pneumonia, and multi-focal endocardial hemorrhages on papillary muscles and wall of ventricle in heart. Histopathological investigation revealed marked congestion and hemorrhages of mesenteric lymph node, liver and spleen. Depletion of lymphocytes from the splenic white pulp was visible in the splenic parenchyma. The virus was confirmed by polymerase chain reaction and phylogenetic analysis revealed a distinct clustering of the Uttar Pradesh virus isolates from Vietnam with other Ib group isolates, indicating a close genetic relationship between these samples. Additionally, the mutant Chinese virus isolate showed clear genetic differences with the Vietnamese Ib group, confirming its suitability as an out-group for comparison. The study represents the first report of ASF outbreak in North India, establishing the phylogenetic relationship between ASF virus circulating in the study area and other regions.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"605-611"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug resistance and virulence-associated genes screening in Salmonella enterica isolated from Caspian pony, Iran. 伊朗里海小马分离的肠炎沙门氏菌的耐药性和毒力相关基因筛选。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2014684.4051

Jamaladdin Eydi, Amir Tukmechi

{"title":"Drug resistance and virulence-associated genes screening in Salmonella enterica isolated from Caspian pony, Iran.","authors":"Jamaladdin Eydi, Amir Tukmechi","doi":"10.30466/vrf.2024.2014684.4051","DOIUrl":"10.30466/vrf.2024.2014684.4051","url":null,"abstract":"The most serious problem in public health is salmonellosis, a common disease in horse. The aim of this study was to investigate the shedding of Salmonella serotypes in healthy Caspian pony. We examined 143 pony's fecal samples collected from the north of Iran belonging to different ages and sexes. Samples were cultured, then identification of isolates were performed by common bacteriological methods and polymerase chain reaction (PCR). The PCR was also used to explore the presence of fimA and salmonella secreted effector L (SseL) genes as virulence factors in the isolates and all were assigned to antibiotic susceptibility test via disc diffusion method. Results showed two fecal samples (1.39%) contaminated with Salmonella and further examination demonstrated the isolates belonging to S. enterica serotype typhimurium. Both serotypes were isolated from female and ˂6 years of age group of ponies and we detected fimA and SseL genes in the isolates. Observing multiple drug resistance and virulence genes in isolates is of utmost importance from both clinical and public health perspectives. It is highly likely that we face instances of salmonellosis in animals or humans that lead to severe infections and fail to respond to treatment in future. This study revealed that the occurrence of Salmonella was low in ponies, however, regarding the presence of virulence factors with multidrug resistant trend in this zoonotic bacterium, establishment of good hygienic measurement to prevent the transmission of bacteria between animal and human is necessary.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"481-486"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection and genetic analysis of Candida species isolated from bovine clinical mastitis in India. 从印度牛临床乳腺炎中分离出的念珠菌物种的分子检测和遗传分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2008702.3968

Nivedha Devanathan, Mouttou Vivek Srinivas, Jayalakshmi Vasu, Shashank Chaluva, Hirak Kumar Mukhopadhyay

{"title":"Molecular detection and genetic analysis of Candida species isolated from bovine clinical mastitis in India.","authors":"Nivedha Devanathan, Mouttou Vivek Srinivas, Jayalakshmi Vasu, Shashank Chaluva, Hirak Kumar Mukhopadhyay","doi":"10.30466/vrf.2024.2008702.3968","DOIUrl":"10.30466/vrf.2024.2008702.3968","url":null,"abstract":"Bovine clinical mastitis is an economically important disease in dairy industry worldwide resulting in reduction of milk yield and quality. Among mycotic mastitis, Candida spp. are commonly occurring opportunistic mycosis in immunocompromised animals. The micro-organism's causing mastitis has high zoonotic potential and has been linked with rapid growth and introduction of antimicrobial resistance between animals and humans. The present study was conducted to isolate and identify the common pathogenic Candida spp. from bovine mastitis cases in India. The isolates were phenotypically characterized by culturing on Sabouraud's dextrose agar, Hichrome Candida differential agar and germ tube production test. Antibiogram was also performed to determine their antifungal activities. The phenotypically positive isolates were confirmed by polymerase chain reaction (PCR) and genetically analyzed by targeting 18S-ITS1-5.8S-ITS2-28S region specific for Candida spp. and identified the yeast at the species level. The antibiogram showed the isolates were highly sensitive with ketoconazole, clotrimazole and miconazole. The PCR assay identified C. lusitaniae and C. tropicalis based on the two distinctive amplicon sizes (592bp and 737bp) respectively. Also, the sequence analysis and phylogeny confirmed C. lusitaniae in six sequences and C. tropicalis in one sequence. It is worth noting that in this study, the species identification was consistent among PCR and genetic analysis. Therefore, the PCR based identification system of the fungal species performed in this study could be an efficient and time saving tool for early diagnosis of clinical mastitis in milch animal, which allows prompt control and application of speedy effective treatment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"509-514"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, purification and immunogenicity analyses of receptor binding domain protein of severe acute respiratory syndrome coronavirus 2 from delta variant. 严重急性呼吸综合征冠状病毒2型δ变异受体结合域蛋白的表达、纯化及免疫原性分析

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2013858.4037

Liqing Liu, Hong Chen, Ping Liu, Xiongfeng Ke, Jingjing Song, Ying Fang, Lingbao Kong, Xiu Xin

{"title":"Expression, purification and immunogenicity analyses of receptor binding domain protein of severe acute respiratory syndrome coronavirus 2 from delta variant.","authors":"Liqing Liu, Hong Chen, Ping Liu, Xiongfeng Ke, Jingjing Song, Ying Fang, Lingbao Kong, Xiu Xin","doi":"10.30466/vrf.2024.2013858.4037","DOIUrl":"10.30466/vrf.2024.2013858.4037","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. The receptor binding domain (RBD), located at the spike protein of SARS-CoV-2, contains most of the neutralizing epitopes during viral infection and is an ideal antigen for vaccine development. In this study, bioinformatic analysis of the amino acid sequence data of SARS-CoV-2 RBD protein for the better understanding of molecular characteristics was performed. The SARS-CoV-2 RBD gene was inserted into pET-28a vector, and efficiently expressed in E. coli system. Then, the recombinant proteins (RBD monomer and RBD dimer protein) were purified as antigen for animal immunization. Furthermore, the results showed that the recombinant proteins (RBD monomer and RBD dimer protein) had adequate immunogenicity to stimulate specific antibodies against the corresponding protein in immunized mice. Taken together, the results of this study revealed that RBD protein had a high immuno-genicity. This study might have implications for future development of SARS-CoV-2 detection.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"657-663"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0