Veterinary Research Forum最新文献_第9页

Antibiotic resistance patterns, characteristics of virulence and resistance genes and genotypic analysis of Salmonella serotypes recovered from different sources. 从不同来源回收的沙门氏菌血清型的抗生素耐药性模式、毒力和耐药性基因的特征以及基因型分析。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2019507.4123

Seyed Mahdi Hosseini, Rahem Khoshbakht, Hami Kaboosi, Fatemeh Peyravii Ghadikolaii

{"title":"Antibiotic resistance patterns, characteristics of virulence and resistance genes and genotypic analysis of Salmonella serotypes recovered from different sources.","authors":"Seyed Mahdi Hosseini, Rahem Khoshbakht, Hami Kaboosi, Fatemeh Peyravii Ghadikolaii","doi":"10.30466/vrf.2024.2019507.4123","DOIUrl":"10.30466/vrf.2024.2019507.4123","url":null,"abstract":"The present study evaluated the Salmonella isolates obtained from various origins in Iran. Salmonella strains previously recovered and stored in the veterinary microbiology laboratory were serotyped and subjected to antibiotic susceptibility test, detection of the virulence and resistance genes by polymerase chain reaction (PCR), and genotyping by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). All Salmonella isolates showed resistance to erythromycin and the most resistance rates were detected for trimethoprim (86.66%), ampicillin (75.00%), and sulfamethoxazole-trimethoprim (63.33%), respectively. In total, 86.33% of the isolates were known as multi-drug resistant and none of the isolates showed resistance to cefepime, nalidixic acid, imipenem, ceftriaxone, and polymyxin B. The virulence genes, invA, sdiA, and hilA besides the tetA resistance gene were identified in all 60 Salmonella strains. The most prevalent resistance genes were respectively tetC (70.00%), sul2 (58.33%), and ereA (55.00%). Statistical analysis revealed a significant difference between Salmonella serotypes associated with the sul1 resistance gene. In ERIC-PCR analysis, 14 distinct clusters were obtained. Statistically, there were significant relationships between the source and ERIC's genomic pattern and between the serotype of Salmonella isolates and genotypic pattern of ERIC. According to the results, Salmonella serotypes from non-human sources had considerable resistance to different antibiotics and carried significant virulence determinants and resistance genes. In addition, ERIC-PCR showed relevant results in discriminating Salmonella serotypes from other sources.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"499-508"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens. 用不同的组织病理学技术研究肉鸡心包积水综合征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2023088.4174

Hossein Alavizadeh, Zahra Moosavi, Gholamreza Hashemi Tabar

{"title":"Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens.","authors":"Hossein Alavizadeh, Zahra Moosavi, Gholamreza Hashemi Tabar","doi":"10.30466/vrf.2024.2023088.4174","DOIUrl":"10.30466/vrf.2024.2023088.4174","url":null,"abstract":"Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran. The dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia and necrotizing enteritis were some of the findings reported in previous research. Low macrophage infiltration rate and brain lesions were other discoveries in Hematoxylin and Eosin (H&E) examination. Feulgen reaction and Cluster of Differentiation 68 (CD68) immunohistochemical staining were used for a comprehensive investigation and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggested that HPS might cause brain damage and the susceptibility of the Arian breed to the adenovirus needs further investigation.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"559-564"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia. 对传染性山羊胸膜肺炎中肥大细胞、白细胞介素 17 和白细胞介素 1β 特征进行评估的免疫组化研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2023580.4184

Ozhan Karatas, Gokhan Akcakavak

{"title":"An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia.","authors":"Ozhan Karatas, Gokhan Akcakavak","doi":"10.30466/vrf.2024.2023580.4184","DOIUrl":"10.30466/vrf.2024.2023580.4184","url":null,"abstract":"Contagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1β protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immuno-histochemical examination, MC profile and local IL-17 and IL-1β protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1β scores were detected in the peracute-acute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1β expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course. Additionally, MC profile was evaluated for the first time in naturally infected CCPP.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"515-521"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic potential of autochthonous Lactobacillus species from buffalo calves in controlling multidrug resistant Escherichia coli. 水牛犊自生乳酸杆菌在控制耐多药大肠杆菌方面的益生潜力

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2024.2012559.4024

Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu

{"title":"Probiotic potential of autochthonous Lactobacillus species from buffalo calves in controlling multidrug resistant Escherichia coli.","authors":"Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu","doi":"10.30466/vrf.2024.2012559.4024","DOIUrl":"10.30466/vrf.2024.2012559.4024","url":null,"abstract":"The aim of this study was to investigate the probiotic potential of autochthonous Lactobacillus species isolated from buffalo calves against multidrug-resistant Escherichia coli. A total of 252 rectal swabs were collected from healthy neonatal buffalo calves under 30 days old from six districts of Andhra Pradesh, India in a completely randomized design from August 2019 to August 2021, of which 190 Lactobacillus strains were isolated based on cultural, morphological, biochemical and molecular tests. Out of 190 isolates, 57 showed high levels of auto-aggregation (> 80.00%) and hydrophobicity (> 60.00%) and 51 of the 57 isolates had a zone of inhibition greater than 15.00 mm in diameter against multidrug-resistant E. coli in an Agar well diffusion assay. Among the 51 isolates, 36 were found to be acid and bile tolerant and showed varying levels of sensitivity to antibiotics such as erythromycin, clindamycin, tetracycline, chloramphenicol, and ampicillin. Among the 36 isolates, Limosilactobacillus reuteri 178, L. reuteri 209, L. fermentum 182, L. fermentum 211, and Lactiplanti-bacillus plantarum 34 were non-hemolytic, and none of the isolates were able to hydrolyse gelatine. Therefore, these five autochthonous Lactobacillus species may be used in probiotic or synbiotic formulations against multidrug resistant E. coli in buffalo calves.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"275-282"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report. 使用卡泊芬净成功治疗白色念珠菌诱发的犬真菌性膀胱炎：病例报告。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2018960.4113

Sei-Myoung Han

{"title":"Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report.","authors":"Sei-Myoung Han","doi":"10.30466/vrf.2024.2018960.4113","DOIUrl":"https://doi.org/10.30466/vrf.2024.2018960.4113","url":null,"abstract":"A 7-year-old castrated male poodle was brought to the referral Animal Medical Center and diagnosed with diabetes and pancreatitis. One month later, the patient presented with cloudy urine, and ultrasonography revealed a large number of spherical substances. The patient was subsequently diagnosed with fungal cystitis with Candida albicans. Initially, 10.00 mg kg-1 itraconazole was prescribed twice daily for six weeks, and the symptoms of prolonged urination improved; however, the fungal balls persisted in the bladder. The six months later, the patient showed recurrent symptoms, such as dysuria and stranguria; therefore, 5.00 mg kg-1 fluconazole was prescribed twice daily; however, it was not effective. Subsequently, 1.00 mg kg-1 caspofungin once daily was administered for three consecutive days. Finally, the fungal balls in the bladder disappeared. The patient was regularly monitored after completion of treatment and, 17 months later, doing well without recurrence. Few reports exist on the use of caspofungin in veterinary medicine. The recommended dose of caspofungin in dogs remains unknown. In the case of azole-resistant Candida, treatment using caspofungin should be considered; although, additional studies on the established dosing and side effects are needed.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"435-438"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial. 在传统牧场系统中对奶牛进行分娩和分娩管理培训的效果：现场试验。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2007348.3945

Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori

{"title":"Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial.","authors":"Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori","doi":"10.30466/vrf.2023.2007348.3945","DOIUrl":"10.30466/vrf.2023.2007348.3945","url":null,"abstract":"The interval between parturition and subsequent pregnancy is called the days open or calving to conception interval and is affected by several factors, especially dystocia. Dystocia is an ab-normal or difficult calving that may require assistance during labor. This study is a field trial in health education and the research team developed a comprehensive training program for farmers to educate them about the normal process of parturition in dairy cows and when and how to assist in parturition or dystocia. A series of classes was held for farmers and the study covered 486 multi-parous dairy cows, with 173 belonging to the group of trained farms (educated farmers) and 313 to the control group (non-educated farmers). Although dystocia was lower in the educated group, there were no significant differences in retained placenta between two groups. However, cows in the educated group had a better conception rate (lower service per conception) in sub-sequent parturitions. Hence, the median number of days open for cows from trained farmers was significantly lower than other farmers (85 days compared to 120 days, respectively). Based on Cox regression analysis, uterine prolapse, retained placenta, and dystocia could significantly impact subsequent pregnancies. Dystocia affects days open, and training on parturition and dystocia management can effectively reduce the numbers of days open in dairy cows.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"139-144"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of biofilm forming fungal species and in vitro evaluation of anti-biofilm activity of disinfectants used in drinking water. 生物膜形成真菌的发生及饮用水消毒剂抗生物膜活性的体外评价。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2016504.4088

Juon Abbass, Muhammad Ashraf, Serpil Kahya Demirbilek, Merve Yıldız, Havva Aner, Ali Raza, Kamil Tayfun Carlı

{"title":"Occurrence of biofilm forming fungal species and in vitro evaluation of anti-biofilm activity of disinfectants used in drinking water.","authors":"Juon Abbass, Muhammad Ashraf, Serpil Kahya Demirbilek, Merve Yıldız, Havva Aner, Ali Raza, Kamil Tayfun Carlı","doi":"10.30466/vrf.2024.2016504.4088","DOIUrl":"10.30466/vrf.2024.2016504.4088","url":null,"abstract":"Fungal contamination in drinking water has garnered considerable attention over the past few decades, especially considering the detrimental consequences of pathogenic fungal species on both human and animal health. The formation of biofilms by certain species is a considerable factor contributing to the emergence of severe fungal infections. This research was designed to isolate and identify fungi, particularly those capable of forming biofilms from 150 samples of drinking water sourced from various locations. The isolated fungal species were tested for them in vitro biofilm formation using a microtitration plate method and the crystal violet assay was applied to quantify the established biofilms. The effectiveness of three disinfectants, namely ozone, chlorine, and hydrogen peroxide, in preventing the formation of biofilms by the most isolated fungal species was monitored. The findings indicated that Aspergillus species were the most prevalent in drinking water, comprising 63.33% (95/150) of the total number of fungal species identified. Aspergillus fumigatus and Aspergillus flavus were identified as the primary contributors to biofilm formation in drinking water distribution systems with prevalence rates of 41.00 and 34.00%, respectively, among all Aspergillus species. The outcomes of the in vitro studies demonstrated that the ozone disinfectant exhibited promising results in inhibiting fungal biofilms compared to chlorine and hydrogen peroxide. In conclusion, these findings provided valuable insights for water distribution authorities to develop effective regimens for controlling biofilm-forming fungal species using suitable antifungal biofilm disinfectants.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"651-656"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of melatonin on the mitogen-activated protein kinase signaling genes in hypoxic Leydig cells. 褪黑素对缺氧间质细胞中丝裂原活化蛋白激酶信号基因的影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2026202.4231

Mona Ghasemi, Adel Saberivand, Mohammadreza Gholami, Vahideh Assadollahi, Masoud Alasvand

{"title":"Effects of melatonin on the mitogen-activated protein kinase signaling genes in hypoxic Leydig cells.","authors":"Mona Ghasemi, Adel Saberivand, Mohammadreza Gholami, Vahideh Assadollahi, Masoud Alasvand","doi":"10.30466/vrf.2024.2026202.4231","DOIUrl":"10.30466/vrf.2024.2026202.4231","url":null,"abstract":"Leydig cells play a crucial role in male reproductive physiology, and their dysfunction is often associated with male infertility. Hypoxia negatively affects the structure and function of Leydig cells. This study aimed to investigate the impact of melatonin on the c-Jun N-terminal kinase (Jnk), P38, and extra-cellular signal-regulated kinases 1 and 2 (Erk1/2) mitogen-activated protein kinase (MAPK) signaling pathways in TM3 mouse Leydig cells under hypoxia induced by cobalt (II) chloride (CoCl2). The TM3 cell line was utilized as a subject of research, and 100 μM CoCl2 was employed to induce hypoxia. Following the addition of 10.00 ng mL-1 melatonin, quantitative reverse transcription-polymerase chain reaction and western blot analyses were conducted to assess the gene expression and protein level of Jnk, p38, and Erk1/2, while enzyme-linked immunosorbent assay was used to measure testosterone secretion. The results showed that melatonin significantly increased testosterone production in the CoCl2 + melatonin group compared to the CoCl2-treated group. Furthermore, melatonin elevated both the protein level and mRNA expression of Erk1/2, Jnk, and p38 genes in the CoCl2 + melatonin group compared to the CoCl2 group. In conclusion, melatonin activated the Jnk, p38, and Erk1/2 MAPK signaling pathways and enhanced testosterone production in the presence of CoCl2 in TM3 cells.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"673-680"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran. 筛查伊朗西北部牛、绵羊和山羊中分离出的耐甲氧西林金黄色葡萄球菌的万古霉素抗性相关基因。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2003964.3902

Leila Maleki, Amir Tukmechi

{"title":"Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran.","authors":"Leila Maleki, Amir Tukmechi","doi":"10.30466/vrf.2023.2003964.3902","DOIUrl":"10.30466/vrf.2023.2003964.3902","url":null,"abstract":"Staphylococcus aureus is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of S. aureus. The MecA gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely vanA and vanB, were searched in the isolates. Out of 400 nasal swabs, 69 samples had S. aureus; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"159-164"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages. 不同胚胎和胎儿阶段鸵鸟（Struthio camelus）气管和肺的形态发生。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2023-06-15 DOI: 10.30466/vrf.2023.2009800.3984

Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar

{"title":"Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages.","authors":"Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar","doi":"10.30466/vrf.2023.2009800.3984","DOIUrl":"10.30466/vrf.2023.2009800.3984","url":null,"abstract":"The ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock industry. This research was conducted to analyze the development of the ostrich respiratory system during fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms and then incubated at 36.00 - 37.00 ˚C and 25.00 ± 2.00% humidity for 40 days. Sections were taken on days 13, 22, 26, 30, 36, and 42 of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by Hematoxylin and Eosin, Alcian Blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques. It was concluded that the trachea in the 13-day-old embryo and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilage and differentiation of mesenchymal to the loose connective tissue. The bronchial stage of the lung was observed in the 22-day-old embryo, pseudoglandular stage in the 26-day-old embryo, and parabrachial and air capillary stage in the 30-day-old embryo. The information obtained from this study will be useful for diagnosing pathologies affecting this vital system and results in improving industrial breeding management.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"297-301"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0