Veterinary Research Forum最新文献

{"title":"Apoptotic activity of Newcastle disease virus in comparison with nisin A in MDA-MB-231 cell line.","authors":"Gholam Abbas Ghorbankhani,&nbsp;Ali Mohammadi,&nbsp;Nasrin Kazemipur,&nbsp;Solmaz Morovati,&nbsp;Behrouz Gharesi Fard,&nbsp;Saeed Nazifi Habibabadi,&nbsp;Mahdi Hashempour Sadeghian","doi":"10.30466/vrf.2022.542258.3297","DOIUrl":"https://doi.org/10.30466/vrf.2022.542258.3297","url":null,"abstract":"<p><p>Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment seems essential. In this study, we evaluated the anti-tumor effects of nisin A and Newcastle disease virus (NDV) on triple-negative MDA-MB-231 cell line. The MDA-MB-231 cell line was separately and in combination subjected to the different concentrations of a Vero-adapted NDV (JF820294.1) and nisin A. The oncolytic effects of these treatments were analyzed by different cytotoxic and apoptosis techniques including trypan blue staining, MTT assay, acridine orange (EB/AO) staining, colony assay and flow cytometry over time. Nisin A at doses of more than 20.00 μg mL^-1 could represent the anti-viral effects and interfere with the oncolytic activity of NDV. Moreover, the analyses indicated that the anti-proliferative and cytotoxic features of combination therapy were stronger than those of individual NDV groups. However, the most apoptotic effect was seen in NDV experimental groups. Taken together, the results from cytotoxicity tests, flow cytometry and colony assay showed that either of the oncolytic agents had significant effects at low concentrations 72 hr post-treatment. Thereby, they had the potential to be used as new approaches in cancer treatment.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 1","pages":"29-37"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/9e/vrf-14-29.PMC9906615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of double intravenous furosemide administration at different doses on clinical variables, electrocardiographic indices and serum electrolytes in miniature donkeys.","authors":"Seyed Hesamaddin Banihashemi,&nbsp;Amir Saeed Samimi,&nbsp;Kamyab Samimi,&nbsp;Javad Tajik","doi":"10.30466/vrf.2020.127810.2954","DOIUrl":"https://doi.org/10.30466/vrf.2020.127810.2954","url":null,"abstract":"<p><p>The intravenous (IV) usage of diuretics such as furosemide may cause changes in clinical variables, electrocardiographic (ECG) indices and some serum electrolytes concentrations in miniature donkeys. The purpose of this study was to evaluate the effects of double intravenous (IV) administration of furosemide at different doses on the clinical variables, ECG indices and serum electrolytes levels in miniature donkeys. A total of 21 clinically and para-clinically healthy miniature donkeys with a mean age of 6.00 ± 1.00 years and weight of 95.00 ± 5.00 kg were used. The animals were randomly divided into three groups of control (normal saline) and low- (1.00 mg kg^-1) and high-dose (2.00 mg kg^-1) of furosemide. Two IV injections with 12-hr interval were administered in each animal. The clinical and ECG parameters were measured at 0 (baseline), 2 (T2), 24 (T24) and 48 (T48) hr after drug administration. Serum concentrations of electrolytes were measured at T0, T24 and T48. The results of this study showed no changes in clinical variables (heart rate, rectal temperature and respiratory rate) and ECG indices (amplitude and duration of P, QRS and T waves and duration of PR, QT and RR intervals) as well as no significant changes in serum electrolytes (sodium, potassium, chloride, phosphorus, calcium and magnesium) levels. Although no significant effect on clinical variables, electrolytes levels and ECG indices after double IV administration of furosemide (1.00 and 2.00 mg kg^-1) in miniature donkeys was observed, absence of these effects does not mean that furosemide had no pharmacological effect.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 1","pages":"53-57"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/a7/vrf-14-53.PMC9906614.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of crocin on experimental gastrocnemius muscle ischemia/reperfusion injury in rat.","authors":"Betul Apaydin Yildirim,&nbsp;Selcuk Albayrak","doi":"10.30466/vrf.2022.548566.3383","DOIUrl":"https://doi.org/10.30466/vrf.2022.548566.3383","url":null,"abstract":"<p><p>Ischemia-reperfusion (IR) injury to the lower extremities causes damage to various tissues, notably the limbs. Because research in recent years have demonstrated that saffron and its components are useful in ischemic strokes, the goal of this study was to see whether Crocin (Cr), one of the active constituents in saffron, could protect the gastrocnemius muscle from IR injury. A total number of 32 Sprague-Dawley rats were randomized into four groups randomly: control, Cr, IR, and IR + Cr. Xylazine and ketamine were used to anesthetize all of the rats. The left lower limbs of the other two groups were subjected to 2 hr of ischemia and 2 hr of reperfusion with tourniquet, with the exception of the control and Cr groups. Tumor necrosis factor alfa (TNF-α), interleukin 6 (IL-6), IL-1β, total antioxidant status (TAS) and total oxidant status (TOS) levels were assessed in the blood as well as muscle IL-6, IL1β, SOD1-2, catalase (CAT) and glutathione peroxidase (GPx) expression. According to the IR group, increases in TAS levels and decreases in TNF-α, IL-6, and IL-1β levels were substantial in the Cr therapy group. Cr significantly reduced IL-6 and IL-1β mRNA expression levels in the muscle of the IR group and increased superoxide dismutases 1 (SOD1), SOD2, catalase (CAT), and GPx. Our data showed that Cr protected the gastrocnemius muscle from IR injury in rats and reduced inflammatory markers significantly. These effects of Cr might have been mediated by improved antioxidant enzyme activity, suppression of free radical generation and reduction of oxidative stress.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 4","pages":"201-206"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/67/vrf-14-201.PMC10170470.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9473970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, virulence factors, and antimicrobial resistance profiles of Shiga toxin-producing <i>Escherichia coli</i> isolated from broiler chickens in Egypt.","authors":"Ibtisam Faeq Hasona,&nbsp;Salwa Mahmoud Helmy,&nbsp;Adel Mohammad El Gamal","doi":"10.30466/vrf.2021.539418.3233","DOIUrl":"https://doi.org/10.30466/vrf.2021.539418.3233","url":null,"abstract":"<p><p>Pathogenic <i>Escherichia coli</i> is one of the world's most important zoonotic foodborne pathogens and poses a serious threat to public health. We examined the prevalence, virulence genes, and antibiotic resistance profile of Shiga toxin (Stx)-producing <i>E. coli</i> (STEC) isolated from broiler chickens in the Kafr El-Sheikh governorate, Egypt. A total of 410 samples (230 cloacal swabs, 180 internal organs) were collected to isolate <i>E. coli</i>. A total of 29 (7.07%) <i>E. coli</i> isolates were recovered and identified, and 18 of them harbored <i>Stx</i> genes (<i>stx</i>). Out of 18 isolates, five (17.24%) carried the <i>stx</i> <i>1</i> gene, five (17.24%) carried the <i>stx</i> <i>2</i> gene, four (13.79%) carried both <i>stx</i> <i>1</i> and <i>stx</i> <i>2</i> genes, and four (13.79%) carried <i>stx</i> <i>1</i> <i>, stx</i> <i>2</i> <i>,</i> and <i>eae</i> <i>A</i> genes. Overall, complete anti-biotic resistance was observed against amoxicillin, ampicillin, cefpodoxime, and cefoperazone; high resistance was observed against ampicillin/sulbactam, nalidixic acid, cefuroxime, aztreonam, ciprofloxacin, ceftriaxone, chloramphenicol, sulfamethoxazole/trimethoprim, and ceftazidime; moderate resistance against gentamicin; low resistance against cefoxitin; lower resistance was detected against norfloxacin, cefotetan, and amikacin; and the lowest resistance against imipenem. All <i>E. coli</i> isolates demonstrated multidrug resistance against at least four antibiotic classes. Out of 29 <i>E. coli</i> isolates, STEC accounted for 18 isolates, of which the O78, O26:H11, O128:H2, O1:H7, O119:H6, and O91:H21 serogroups were predominant. All <i>E. coli</i> isolates were multidrug resistant and therefore pose a potential public health concern as these virulent, resistant strains may spread to humans. Thus, high levels of hygiene and biosecurity are required by chicken handlers to decrease the danger of infection spreading to humans.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 3","pages":"131-138"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1c/6d/vrf-14-131.PMC10073808.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights on skewing of sex ratio in rabbits (<i>Oryctolagus cuniculus</i>) supplemented with dietary calcium and magnesium.","authors":"Sharanya Jeevendra Naidu,&nbsp;Arangasamy Arunachalam,&nbsp;Akeem Babatunde Sikiru,&nbsp;Selvaraju Sellappan,&nbsp;Backialakshmi Sekar,&nbsp;Ippala Janardhan Reddy,&nbsp;Raghavendra Bhatta","doi":"10.30466/vrf.2022.551122.3428","DOIUrl":"https://doi.org/10.30466/vrf.2022.551122.3428","url":null,"abstract":"<p><p>The effect of dietary calcium (Ca) and magnesium (Mg) supplementation on serum biochemical parameters, steroid hormones, gene expression, and the sex ratio was investigated in female New Zealand white rabbits. A total of 25 rabbits were allocated into five treatment groups: The control group was fed with regular pellet feed, whereas, treatment groups were supplemented with Ca and Mg: T1 (0.40% and 0.01%), T2 (0.60% and 0.02%), T3 (0.80% and 0.03%) and T4 (1.00% and 0.04%), respectively. The rabbits were subjected to three breeding cycles. The T3 group skewed towards females (65.33%) from all three breeding. There was elevated Ca concentration in T3 (15.26 ± 0.77 mg dL^-1) and T4 (15.61 ± 0.82 mg dL^-1) groups compared to the control. The concentration of estradiol was significantly high in T3 and T4 groups at 0.5 days post-coitus (dpc) and T2, T3 and T4 groups at 21dpc. Testosterone was significantly high in T4 group at 0.50 dpc and T2 and T4 group at 21dpc. The expression of 13 genes was studied in the oviduct. Genes such as <i>OVGP1, CCT4, ANXA2</i> and <i>TLR4</i> were up-regulated and positively correlated with the female sex ratio. The molecular functions and pathways of up-regulated genes were suggestive of their role in fertilization such as sperm selection, sperm storage, immune regulation, implantation and early embryonic development. The variations in the serum electrolytes, steroid hormones and gene expression might have an impact on the skewing process.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 8","pages":"405-413"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/94/d2/vrf-14-405.PMC10475169.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10217299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livestock, pets and humans as carriers of methicillin-resistant <i>Staphylococcus</i><i>aureus</i> and comparative evaluation of two PCR protocols for detection.","authors":"Dharitree Sonowal,&nbsp;Sandeep Ghatak,&nbsp;Acheenta Gohain Barua,&nbsp;Srinivas Kandhan,&nbsp;Razibuddin Ahmed Hazarika,&nbsp;Arnab Sen,&nbsp;Samir Das,&nbsp;Sarat Sonowal,&nbsp;Rajeev Kumar Sharma,&nbsp;Shantanu Tamuly,&nbsp;Chimanjita Phukan,&nbsp;Ajanta Sharma,&nbsp;Poznur Hussain","doi":"10.30466/vrf.2023.1971891.3672","DOIUrl":"https://doi.org/10.30466/vrf.2023.1971891.3672","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> are Gram positive bacteria known to acquire antibiotic resistance rapidly and pose a major challenge to clinicians worldwide. Infections by methicillin resistant <i>Staphylococcus aureus</i> (MRSA) are usually associated with increased mortality and prolonging of treatment. Samples (n = 706) from diverse sources (livestock, pets, animal handlers, human hospital) were collected and screened for the presence of MRSA by phenotypic and genotypic methods. The incidence of <i>Staphylococcus aureus</i> was greater in goats (42.00%; 28.20 - 56.80%, confidence interval [CI] 95.00%) followed by cattle (13.50%; 9.20 - 18.80%, CI 95.00%), humans (12.90%; 9.30 - 17.40%, CI 95.00%) and dogs (12.90%; 8.10 - 19.20%, CI 95.00%). Significantly higher incidence of MRSA was observed in dogs (65.00%; 40.80 - 84.60%, CI 95.00%), compared to other hosts namely cattle (48.00%; 26.50 - 64.30%, CI 95.00%), humans (35.00%; 20.20 - 52.50%, CI 95.00%) and goats (10.00%; 1.20 - 30.40%, CI 95.00%). All the <i>S. aureus</i> isolates were further screened for thermostable nuclease (<i>nuc</i> gene) by polymerase chain reaction (PCR). The incidence of <i>nuc</i> gene in cattle, dog, goat and human were found to be 3.30% (1.30 - 6.60%, CI 95.00%), 5.20% (2.30 - 9.90%, CI 95.00%), 28.00% (16.20 - 42.50%, CI 95.00%) and 9.10% (6.00 - 13.00%, CI 95.00%), respectively. Comparative evaluation of two PCR primers (<i>mecA</i>-162 and <i>mecA</i>-310) indicated the former one as more rational choice for detection of MRSA. Overall, the results of our study indicated possible risk of zoonotic transmission of MRSA from canines.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 7","pages":"351-358"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/04/vrf-14-351.PMC10410111.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progesterone circulation and follicular growth in adapted <i>Bos taurus</i> cows.","authors":"Héctor Javier Narváez,&nbsp;Luis Ernesto Quintero Rodríguez","doi":"10.30466/vrf.2022.554987.3489","DOIUrl":"https://doi.org/10.30466/vrf.2022.554987.3489","url":null,"abstract":"<p><p>The creole breed Blanco Orejinegro (<i>Bos taurus</i> adapted) is an important animal genetic resource in Colombia. However, very little is known about the reproductive physiology of the breed. The objective was to determine the dynamics of progesterone circulation and follicular growth in Blanco Orejinegro cows throughout the estrous cycle. Fifteen cyclic Blanco Orejinegro cows were used and subjected to hormonal protocol for estrous and ovulation synchronization. Once the time of ovulation was identified by monitoring the ovarian dynamics with ultrasonography equipment. For description of the results, the mean was used as a measure of central tendency and the standard error of the mean. Statistical analysis was longitudinal descriptive. Blood samples were collected every 24 hr throughout the estrous cycle for serum quantification of progesterone (P4), monitoring of corpus luteum (CL) growth dynamics and follicular dynamics. It was possible to verify that 76.92% of the Blanco Orejinegro cows presented two follicular waves during the estrous cycle and the highest circulating levels of P4 (> 6.00 ng mL^-1) were observed on the 14^th day of the estrous cycle. It was concluded that the creole cows of the Blanco Orejinegro breed presented two follicular waves per estrous cycle. In addition to, the size of the CL was consistent with the secretion of progesterone presenting higher circulating levels at the end of the luteal phase.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 7","pages":"397-400"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/e2/vrf-14-397.PMC10410107.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ovariohysterectomy on queen myocardial function: echocardiographic evidence.","authors":"Boshra Elyasi,&nbsp;Mohammad Nasarolahzade Masouleh,&nbsp;Alireza Jahandideh","doi":"10.30466/vrf.2022.552942.3456","DOIUrl":"10.30466/vrf.2022.552942.3456","url":null,"abstract":"<p><p>Ovariohysterectomy (OHE) is one of the most common surgeries in veterinary medicine. Although this surgical method has several advantages, it can alter the function of various organs such as heart. The present study investigated the effect of OHE on cardiac functions using M-mode echocardiography. A total of 10 healthy adult domestic short-haired cats were enrolled in the current study. Fractional shortening (FS) and ejection fraction (EF) percentages along with cardiac output (CO) were measured through the right parasternal approach in papillary muscle level view. Moreover, the levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by enzyme-linked immunosorbent assay before OHE (D0) as well as 10 (D10), 20 (D20) and 30 (D30) days after OHE. The results of this study showed that the mean FS and EF decreased on all days of the study. The FS reduction was significant between D10 and D30 and EF changes were significant between D10 and D20. The means of CO increased significantly on D0 compared to the D10. After D20, CO reduced until the end of the study. Mean concentrations of LH and FSH increased on all research days; but, the changes were significant until D20. Despite the negative effects of OHE on myocardial function, there was no significant correlation between hormonal levels and echocardiographic findings after OHE in this study.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 9","pages":"495-499"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/42/vrf-14-495.PMC10560328.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evaluation of sheep and goats isolates of <i>Pasteurella multocida</i> and their antibiotic resistance.","authors":"Mohammad Tabatabaei,&nbsp;Fatemeh Abdolahi","doi":"10.30466/vrf.2022.556438.3524","DOIUrl":"10.30466/vrf.2022.556438.3524","url":null,"abstract":"<p><p><i>Pasteurella multocida</i> exists as a commensal in the upper respiratory tracts of livestock, and poultry, and causes a wide variety of diseases in humans and animals. This study aimed to investigate the incidence of <i>P. multocida</i> by bacteriological and molecular characterization in sheep and goats and screening the existence of capsule-specific genes and their antibiotic resistance pattern. Totally, 1650 nasopharyngeal swabs were collected from apparently healthy sheep and goats and 460 lung tissues were collected from slaughtered animals in Fars province, Iran. All samples were cultured and suspected colonies were examined by biochemical tests, antimicrobial assay and polymerase chain reaction (PCR). Among 165 <i>P. multocida</i> (104 sheep and 61 goats) isolates, the <i>capA</i>, <i>capD</i>, and <i>capB</i> genes were amplified in 98, 48, and 12 isolates, respectively. The occurrence of four virulence-associated genes of <i>P. multocida</i> isolates were determined by PCR. Most isolates harbored the <i>toxA</i> (79.40%) and <i>hgbB</i> genes (70.90%) and 59.40% of isolates had the <i>pfhA</i> gene. Almost half of the isolates (46.10%) contained the <i>tbpA</i> gene. According to the current study, <i>P. multocida</i> capsular type A had the most frequency followed by type D. In addition, the high frequency of <i>tbpA</i>, <i>pfhA</i>, <i>toxA</i>, and <i>hgbB</i> genes revealed that these genes are possibly important in the pathogenesis of <i>P. multocida</i>. Oxytetracycline, enrofloxacin, florfenicol, and tilmicosin were the most effective drugs.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 9","pages":"481-487"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/80/vrf-14-481.PMC10560327.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of clinical and diagnostic imaging findings of bilateral superficial digital flexor tendon luxation in the tarsus of a gelding.","authors":"Amin Anoushepour,&nbsp;Saeideh Eftekhari,&nbsp;Majid Masoudifard","doi":"10.30466/vrf.2023.1989381.3780","DOIUrl":"https://doi.org/10.30466/vrf.2023.1989381.3780","url":null,"abstract":"<p><p>Superficial digital flexor tendon (SDFT) plays an important role in the locomotion of the horses with flexion of the limbs. In the hind limb, it passes over the calcaneal bone and attaches to medial and lateral surfaces of calcaneous by two bands of connective tissue; while, the medial band is weaker than the lateral one. Occasionally, severe trauma, over-extension or fracture of the calcaneous may cause rupture of the supportive bands leading to tendon luxation. An 11-year-old Thoroughbred jumping gelding with symptoms of lameness in both legs, after examination by an internal specialist veterinarian in equine practice field was referred to the Teaching and Research Hospital of the Faculty of Veterinary Medicine of the University of Tehran, Tehran, Iran. Clinical examination showed lateral luxation of SDFT in the right and left tarsal joints. Radiography revealed no sign of calcaneal involvement, except soft tissue swelling. In the radiography of the tarsal joints, the calcaneus bones did not show significant changes, but among the bones of the central and 3^rd tarsal bone (distal intertarsal joint) of the tarsus, osteophyte formation was observed to a mild extent, indicating mild bone spavin in both tarsal joints. In ultrasonography, SDFT thickenings accompanied by hypoechoic regions in tendons were seen, confirming the signs of tendonitis. Moreover, fibrous tissue formation was diagnosed. In the left leg, anechoic fluid in the calcaneous bursa was seen. Evaluation of diagnostic imaging findings enables veterinarians to diagnose the site and extent of the lesions, choosing the appropriate therapeutic approach in addition to clinical symptoms.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"14 10","pages":"579-582"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71414298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}