MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression.

IF 0.8 4区 农林科学 Q3 ZOOLOGY
Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI:10.30466/vrf.2024.2022533.4165
Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran
{"title":"MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression.","authors":"Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran","doi":"10.30466/vrf.2024.2022533.4165","DOIUrl":null,"url":null,"abstract":"<p><p>Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-142-3p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-142-3p was initially assessed in MDA-MB-468 cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-142-3p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-142-3p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX2, Octamer 4, HMGA2, Kruppel-like factor 4, and Bach-1. Additionally, the combination of miRNA-142-3p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G<sup>1</sup> phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-142-3p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-142-3p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"629-643"},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725297/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Forum","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.30466/vrf.2024.2022533.4165","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-142-3p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-142-3p was initially assessed in MDA-MB-468 cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-142-3p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-142-3p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX2, Octamer 4, HMGA2, Kruppel-like factor 4, and Bach-1. Additionally, the combination of miRNA-142-3p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G1 phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-142-3p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-142-3p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.

MicroRNA-142-3p 使乳腺癌对多西他赛化疗敏感:诱导细胞凋亡和细胞周期停滞,并抑制迁移。
多西紫杉醇(Docetaxel, DTX)广泛应用于乳腺癌治疗。然而,癌细胞的耐药性限制了其抗肿瘤作用。一些被称为microrna (mirna)的分子,就像微调开关一样,可以影响乳腺癌的发展和扩散。我们进行了一项研究,以检验用一种被称为miRNA-142-3p的特殊分子来增加乳腺癌细胞是否可以提高一种被广泛使用的治疗方法DTX的疗效。初步评估MDA-MB-468细胞中miR-142-3p的表达水平。转染miRNA进行进一步实验。研究了DTX和miRNA-142-3p联合处理对细胞迁移(通过伤口愈合试验)和凋亡(通过膜联蛋白V/碘化丙啶染色)的影响。MTT法测定细胞活力,实时定量聚合酶链反应测定基因表达。DTX与miRNA-142-3p联合应用后,促进肿瘤生长的SOX2、Octamer 4、HMGA2、Kruppel-like factor 4、Bach-1等因子的表达显著降低。此外,miRNA-142-3p和DTX联合启动凋亡细胞死亡。此外,通过诱导细胞周期阻滞在G1期,乳腺癌细胞的进展受到阻碍。这种组合也有效地抑制了乳腺癌细胞的迁移和侵袭。DTX或miRNA-142-3p单独可以抑制乳腺癌细胞的恶性行为和进展,但它们的联合产生协同效应,进一步增强对乳腺癌的抑制作用。综上所述,miRNA-142-3p转染可与DTX治疗联合使用,以增强其对乳腺癌细胞的细胞毒性并防止化疗耐药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinary Research Forum
Veterinary Research Forum Veterinary-General Veterinary
CiteScore
1.50
自引率
0.00%
发文量
0
审稿时长
8 weeks
期刊介绍: Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信