Veterinary Research Forum最新文献

Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry. 伊朗卡尚地区肉鸡4型禽腺病毒的分子和组织病理学特征：对家禽业疾病控制和预防的意义

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030590.4302

Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi

{"title":"Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry.","authors":"Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi","doi":"10.30466/vrf.2024.2030590.4302","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030590.4302","url":null,"abstract":"Fowl adenovirus (FAdV) is a DNA virus causing significant diseases, like inclusion body hepatitis, hydropericardium-hepatitis syndrome (HHS), and gizzard erosion. These diseases lead to severe economic losses in the poultry industry. Recent increases in HHS outbreaks in Iran, particularly among broilers, prompted this study to analyze FAdV isolates in Kashan, Iran. In December 2021, a high-mortality HHS outbreak in a Kashan broiler flock led to liver and heart samples being sent for analysis. Histopathological investigations revealed mononuclear hepatitis and intra-nuclear viral inclusion bodies in hepatocytes. Polymerase chain reaction and phylogenetic analyses confirmed the presence of FAdV-4 (accession number: PP856395), showing 99.99% identity with strains from Japan, the United Arab Emirates, Pakistan, and the United States. These findings highlight the genetic similarity and potential common origin of FAdV-4 strains. This study emphasizes the need for heightened biosecurity measures and effective vaccination strategies to mitigate the spread of FAdV-4. The confirmed presence of FAdV-4 in central Iran poses a significant threat to the poultry industry, necessitating prompt action to prevent substantial economic losses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"19-25"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine. 黑腿病疫苗抗体检测用邵氏梭状芽胞杆菌细胞表面蛋白的纯化方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2023812.4190

Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri

{"title":"A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine.","authors":"Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri","doi":"10.30466/vrf.2024.2023812.4190","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023812.4190","url":null,"abstract":"Cell-surface proteins of Clostridium chauvoei were purified using a simple method. Bacterial cultures were centrifuged and agitated vigorously in phosphate buffered saline with or without further glycine treatment and ammonium sulfate precipitation. Rabbits were immunized subcutaneously with a blackleg disease vaccine twice with a two-week interval. Immunized sera were collected one week after the second injection. Enzyme-linked immunosorbent assay (ELISA) was performed using the proteins purified by the second method as the coating antigen. Bradford assay results showed a higher protein concentration in the second than the first method. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis analysis showed multiple bands for the cell-surface proteins of C. chauvoei in the first method and a sharp band equivalent to flagellin protein in the second method. The ELISA results indicated that the purified proteins were capable of detecting antibodies against Blackleg disease vaccine. The purified protein would be an alternative antigen for indirect ELISA in order to monitor the immune response in vaccinated farm animals.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"57-61"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats. 百里醌通过抗氧化和抗炎机制减轻大鼠爪切引起的自发性和诱发性疼痛。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2025053.4204

Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh

{"title":"Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats.","authors":"Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh","doi":"10.30466/vrf.2024.2025053.4204","DOIUrl":"10.30466/vrf.2024.2025053.4204","url":null,"abstract":"Surgery commonly causes post-operative pain that should be alleviated to prevent complications. In addition to the use of synthetic drugs, there has been a widespread desire to use medicinal plants for surgical pain management. Thymoquinone (TQ), a constituent of Nigella sativa black seeds, exhibits a potent anti-oxidant property. Celecoxib (CLX), a potent non-steroidal anti-inflammatory drug, is widely used in pain management. In the present study, the effects of TQ and CLX on pain caused by hind paw surgical incision were compared. Fifty-six rats were divided into four groups of 14 rats as intact, vehicle, TQ, and CLX groups. In each group, six rats were planned to record pain-related behaviors on days 1 - 10 and eight rats were designed for determination of serum biochemical alterations on days 1 (four rats) and 3 (four rats) after surgery. Oral administrations of TQ and CLX at a same dose of 10.00 mg kg-1 alleviated paw lifting number (spontaneous pain) and paw withdrawal threshold evoked by von Frey filaments on metal mesh floor, improved the decreased contents of serum total anti-oxidant capacity and superoxide dismutase, and restored the increased levels of serum malondialdehyde and tumor necrosis factor-alpha. The results suggested that TQ by employing anti-oxidant and anti-inflammatory mechanisms, might relieve the pain induced by hind paw plantar incision, being comparable with CLX.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"89-96"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture. 聚β-羟基丁酸对鲤鱼鱼种的促生长潜力及免疫刺激作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2024674.4200

Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz

{"title":"Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture.","authors":"Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz","doi":"10.30466/vrf.2024.2024674.4200","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024674.4200","url":null,"abstract":"The natural polymer poly-β-hydroxybutyrate (PHB) is converted to β-hydroxybutyric acid, which is similar to short-chain fatty acids, via microbial fermentation and host enzyme breakdown. This study investigated the impact of different PHB concentrations (Control, 1.00, 3.00, and 5.00% substitution) on growth performances and fish welfare in common carp fingerlings. After a 60-day trial, fish fed on diet containing 1.00% PHB exhibited significantly higher weight gain and improved feed conversion efficiency compared to the control group. Furthermore, analysis of enzymatic activity showed elevated levels of total protease and amylase in PHB-fed treatments compared to the control. Red blood cell counts, hemoglobin, and hematocrit levels remained unaffected and a significant increase in white blood cell count was observed in fish fed on diets containing 1.00 and 3.00% PHB compared to the control group. Furthermore, fish fed on diets containing 1.00 and 3.00% PHB demonstrated significantly higher total protein levels and lower glucose concentrations as well as reduced hepatic enzyme activities (aspartate aminotransferase and alanine aminotransferase) compared to both the control and 5.00% PHB groups. Assessment of antioxidant and immune parameters revealed significantly increased complement hemolytic activity and immunoglobulin M levels coupled with decreased malondialdehyde concentrations in the plasma of PHB-fed fish compared to the control group. In conclusion, dietary supplementation with PHB, especially at the 1.00% level, enhanced growth performance and improved nutritional and health indicators in the fingerlings. These findings suggested that PHB had the potential to be a valuable dietary additive for this species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"43-50"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing feed efficiency and egg production in Hy-Line W-80 and Iraqi indigenous chickens. 海兰W-80和伊拉克土鸡饲料效率和产蛋率的比较。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 DOI: 10.30466/vrf.2024.2030349.4297

Dhafer Abdul-Malek Ali, Nihad Abdul-Lateef Ali, Mohammed Baqur Sahib Al-Shuhaib

{"title":"Comparing feed efficiency and egg production in Hy-Line W-80 and Iraqi indigenous chickens.","authors":"Dhafer Abdul-Malek Ali, Nihad Abdul-Lateef Ali, Mohammed Baqur Sahib Al-Shuhaib","doi":"10.30466/vrf.2024.2030349.4297","DOIUrl":"10.30466/vrf.2024.2030349.4297","url":null,"abstract":"The Iraqi indigenous chicken (IIC) is a native breed known for its remarkable ability to thrive in harsh environments. However, the primary challenge with this breed is its lower productivity levels especially when compared to commercial strains. This study evaluated the performance of 120 layers of IIC in comparison with an equal number of Hy-Line W-80 layers across multiple traits including feed efficiency (FE), feed conversion ratio (FCR), daily feed intake (DFI), egg mass (EM) and total egg number (EN) production. Over a period spanning four consecutive weeks intervals significant differences were observed between the two lines across all measured parameters. Hy-Line W-80 consistently exhibited superior FE, with values ranging from 0.49 to 0.54 compared to IIC values that ranged from 0.23 to 0.26. Similarly, Hy-Line W-80 demonstrated lower FCR values ranging from 1.85 to 2.03 contrasting with IIC (3.77 to 4.37). The DFI was notably higher in Hy-Line W-80 (ranging from 104.30 to 106.00 g per day) compared to IIC (73.75 to 90.20 g per day). Furthermore, Hy-Line W-80 consistently outperformed IIC in EM production with values ranging from 52.33 to 56.67 compared to IIC values (19.83 to 21.47). Another superiority of Hy-Line W-80 was observed by showing higher EN over IIC layers in all sixteen weeks of investigation. In conclusion, Hy-Line W-80 consistently outperformed IIC in various parameters such as FE, FCR, DFI, EM and EN production. Accordingly, these data could be exploited in selective breeding and genetic improvement strategies to enhance the productivity of IIC.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"195-201"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro evaluation and efficacy of bacteriophage isolated from commercial poultry farms against Salmonella typhimurium. 商业家禽养殖场分离的噬菌体抗鼠伤寒沙门菌的体外评价和效果。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2030436.4305

Amina Zaib, Ali Ahmad Sheikh, Faisal Ayub, Muhammad Arshad Durrani, Mobashra Mustafa, Afifa Shahzad, Urooj Ejaz, Muhammad Zubair Latif, Chamman Zahra, Izza Izza, Fareeha Zaib, Husnain Ahmad, Muhammad Jawad Hafeez, Muhammad Mujahid, Hafiz Muhammad Moavia Atique

{"title":"In-vitro evaluation and efficacy of bacteriophage isolated from commercial poultry farms against Salmonella typhimurium.","authors":"Amina Zaib, Ali Ahmad Sheikh, Faisal Ayub, Muhammad Arshad Durrani, Mobashra Mustafa, Afifa Shahzad, Urooj Ejaz, Muhammad Zubair Latif, Chamman Zahra, Izza Izza, Fareeha Zaib, Husnain Ahmad, Muhammad Jawad Hafeez, Muhammad Mujahid, Hafiz Muhammad Moavia Atique","doi":"10.30466/vrf.2024.2030436.4305","DOIUrl":"10.30466/vrf.2024.2030436.4305","url":null,"abstract":"Avian Salmonellosis impacts the economy and public health, with chicken products being a major cause of gastroenteritis. Hygiene, immunization and medicines are all used as control techniques. Bacteriophages provide a safe, targeted alternative. In the present study in vitro evaluation of bacteriophages were done against Salmonella typhimurium. Lytic effect of bacteriophages isolated from poultry sludge was checked on culture of S. typhimurium. Stability study was checked at range of temperature and pH. The phages were stable at temperature (30.00 - 50.00 ˚C) and pH (5.00 - 9.00) where best activity was seen at 37.00 ˚C and pH 7.00. In vitro lytic activity was done at (optical density 600 nm) after exposure to bacterial host at different intervals. Multiplicity of Infection of 1.00 was used to check lytic activity of phages which indicated phages were potent enough to infect bacterial cells within their growth cycle. The percentage of unadsorbed phages was determined by bar chart analysis. The genome of three phages was treated with DNase I where they all were sensitive. Later the nucleic acid of phages was digested by restriction endonucleases (EcoRI and HindIII) both of the enzymes produced various restriction sites with different band. The present study proved that the application of bacteriophages in vitro into bacterial system i.e., S. typhimurium was an attractive method in diminishing infection in commercial poultry thus providing exceptional results that could be used on a large scale.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"267-275"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody. 基于免疫球蛋白Y抗体的口蹄疫病毒A血清型特异性抗体固相竞争酶联免疫吸附检测方法的建立。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2025842.4226

Zahra Ivani, Behzad Hemati, Naser Harzandi, Seyed Mahmoud Azimi, Mohammad Mehdi Ranjbar

{"title":"Development of solid phase competitive enzyme-linked immunosorbent assay to detect foot-and-mouth disease virus A serotype specific antibodies based on immunoglobulin Y antibody.","authors":"Zahra Ivani, Behzad Hemati, Naser Harzandi, Seyed Mahmoud Azimi, Mohammad Mehdi Ranjbar","doi":"10.30466/vrf.2024.2025842.4226","DOIUrl":"10.30466/vrf.2024.2025842.4226","url":null,"abstract":"The aim of this study was to using purified chicken antibody (IgY) for developing solid phase competitive (SPC) enzyme-linked immunosorbent assay (ELISA) to detect the foot-and-mouth disease virus (FMDV) A serotype. After immunization of chickens, polyclonal immunoglobulin (IgY) antibodies were extracted and purified from egg yolk and yield was about 5.00 mg mL-1 of yolk as well as near 0.40 mg mL-1 of specific IgY antibody against FMDV serotype A. Also, optimized sucrose density gradient method produced 228 µg mL-1 whole virus which is much higher than that of the conventional method of sucrose density gradient method. The optimum concentration of purified capture IgY and bind type A antigen were 0.50 µg and 0.10 µg per well, respectively. The OD values < 0.70 were considered positive, and values ≥ 0.70 were negative for in-house kit base on standard controls. Statistical analysis base on 80 serum samples showed the 96.66% sensitivity, 100% specificity, 100% positive predictive value, 90.90% negative predictive value, 97.50% accuracy, and 98.33% reliability for serum samples for two commercial and in-house kits. The SPCE developed based on IgY antibody is a suitable alternative for the detection of antibodies after vaccination against type A FMDV with high sensitivity and specificity. The present research demonstrated the possibility of commercial development of the SPCE kit using IgY antibodies for the detection of FMDV antibodies in serum samples with adequate sensitivity and accuracy.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"285-292"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis. 在类风湿性关节炎实验模型中将胡椒碱与泼尼松龙结合使用的益处

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2031860.4316

Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid

{"title":"Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis.","authors":"Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid","doi":"10.30466/vrf.2024.2031860.4316","DOIUrl":"10.30466/vrf.2024.2031860.4316","url":null,"abstract":"This study evaluated the impact of combining piperine and prednisolone on clinical symptoms and immune responses in Wistar rats with rheumatoid arthritis (RA) induced by Freund's complete adjuvant due to piperine known anti-inflammatory and immunomodulatory properties. The RA rats were randomly divided into five groups (n = 10): The RA rats were treated with phosphate-buffered saline, RA rats treated with piperine (100 mg kg-1 orally), RA rats treated with prednisolone (10.00 mg kg-1 orally), and RA rats treated with a combination of piperine and prednisolone (half doses of each orally). Treatment started on day five post-induction when all rats had a clinical score of ≥ 1. Disease symptoms were monitored every other day until day 23 post-induction. Combining the two medications at half doses led to a more significant reduction in disease severity, weight improvement, and histopathological changes compared to using each drug alone at the full doses. The combined treatment group exhibited the most favorable response in C-reactive protein, myeloperoxidase, and nitric oxide biochemical tests compared to the other treatment groups. The combined treatment group showed decreased expression of T-bet and RORɣt genes. However, there was no statistically significant difference in the expression of Foxp3 and GATA3 genes compared to the group receiving prednisolone alone. Overall, combining piperine with prednisolone may prove to be a beneficial approach for managing RA.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"117-124"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the reference values for arterial blood gas, electrolytes and acid-base in healthy male sheep of Iranian Shall breed. 伊朗Shall种健康雄性绵羊动脉血气、电解质和酸碱参考值的测定。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.30466/vrf.2024.2028629.4270

Sirous Sadeghian Chaleshtori, Mohammad Reza Mokhber Dezfouli, Abolfazl Najd Ghahremani, Neda Sabetzadeh

{"title":"Determining the reference values for arterial blood gas, electrolytes and acid-base in healthy male sheep of Iranian Shall breed.","authors":"Sirous Sadeghian Chaleshtori, Mohammad Reza Mokhber Dezfouli, Abolfazl Najd Ghahremani, Neda Sabetzadeh","doi":"10.30466/vrf.2024.2028629.4270","DOIUrl":"10.30466/vrf.2024.2028629.4270","url":null,"abstract":"The goal of this research was to determine the normal range of arterial blood gas, electrolytes and acid-base status in the healthy male sheep of Iranian Shall breed. For this purpose, the blood sample was collected from the ear artery of 15 Iranian Shall sheep whose health was confirmed by clinical examinations, hematological tests including complete blood count and air-dried blood smear and cardiac evaluations including electrocardiography and echocardiography. Then, the values of pH, partial dioxide carbon pressure, partial oxygen pressure, oxygen saturation, base excess, bicarbonate as well as the concentration of electrolytes and anion gap were measured using a blood gas analyzer. The obtained data were subjected to statistical analyses by SPSS software and the tests of Kolmogorov-Smirnov and Pearson and descriptive statistics of the results were expressed as mean ± SE, range (minimum and maximum) and confidence interval 95.00%. The results showed the mean value of arterial blood gas including oxygen saturation, partial oxygen pressure, and partial dioxide carbon pressure were 95.69%, 61.58, and 26.28 mmHg, respectively, and the others arterial blood parameters including pH, base excess, and bicarbonate were 7.54, 1.61, and 22.84 mmol L-1, respectively. Also, the mean values of arterial blood electrolytes including Na+, K+, and Cl-, were 143.25, 4.22, and 109.46 mmol L-1, respectively, and the mean anion gap was 12.64 mmol L-1. The present study determined the range of normal amounts of arterial blood gas, electrolytes and acid-base status in the healthy male sheep of Iranian Shall breed which could be used as a reference for further research on sheep diseases and to distinguish healthy status from disease.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"175-179"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent multi-systemic eosinophilic epitheliotropic disease and choledocholithiasis in a 15-year-old mixed-breed mare. 15岁混血母马并发多系统嗜酸性嗜上皮性疾病和胆总管结石。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.30466/vrf.2024.2034996.4356

Zahra Baradaran-Seyed, Mohammad Eslampanah

{"title":"Concurrent multi-systemic eosinophilic epitheliotropic disease and choledocholithiasis in a 15-year-old mixed-breed mare.","authors":"Zahra Baradaran-Seyed, Mohammad Eslampanah","doi":"10.30466/vrf.2024.2034996.4356","DOIUrl":"10.30466/vrf.2024.2034996.4356","url":null,"abstract":"A mixed-breed 15-year-old mare was euthanized for recurrent colic, severe weight loss, pastern ulcerative dermatitis, and coronitis without response to non-surgical medical procedures. The liver was congested with firm consistency at necropsy, and there were multi-focal white spots on Glisson's capsule. Hemorrhage, ulcer, severe folding, and hypertrophy were seen in the duodenum and jejunum. Histopathological examination showed marked infiltration of eosinophils concomitant with fewer leukocytes, macrophages, and plasma cells, primarily in the liver and intestines. The presence of eight calcium bilirubinate stones, 2.00 - 5.00 cm in diameter, in the common bile duct was characteristic of choledocholithiasis. Concurrent multi-systemic eosinophilic epitheliotropic disease (MEED) and choledocholithiasis were confirmed after ruling out other potential causes of eosinophilic infiltration, including parasitic infection and allergy. The cause of MEED and choledocholithiasis was unclear because of incomplete case history and delayed referral, with a potential infection playing a role and having synergistic effects between the two conditions. When eosinophilic-associated inflammation involves several organs, MEED should be on the differential diagnostic list since infections and allergens are also among the risk factors for this disorder.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"305-308"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0