Veterinary Research Forum最新文献

Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice. 比较某些氨基糖苷类抗生素对小鼠运动行为的影响

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2001972.3891

Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin

{"title":"Comparison of the effects of selected aminoglycoside antibiotics on motor behaviors in mice.","authors":"Seyed Ali Ayati Najafabadi, Ali Rassouli, Goudarz Sadeghi-Hashjin","doi":"10.30466/vrf.2023.2001972.3891","DOIUrl":"10.30466/vrf.2023.2001972.3891","url":null,"abstract":"Aminoglycoside antibiotics (AGs) can cause neuromuscular blockade and paralysis of skeletal muscles. To compare the paralytic effects of selected AGs on some motor behaviors in mice, 24 male mice were divided into four groups. Each group was given one of AGs (gentamicin, dihydro-streptomycin, apramycin and amikacin) at incremental doses that increased half-logarithmically compared to the therapeutic dose (16.00 mg kg-1). Motor behavioral tests included open field test, inclined plane, horizontal bars, static rods, parallel bars and rotarod. Finally, the data were analyzed using descriptive and analytical statistics. Gentamicin and dihydrostreptomycin at 32.00 times of the therapeutic dose produced complete paralysis of the limbs, respiratory arrest, and even death in some animals. However, apramycin and amikacin did not show significant effects on skeletal muscle and motor behaviors at 32.00 times of the therapeutic dose. After administration of apramycin at 100 times of the therapeutic dose, four out of six mice (66.67%) died from respiratory depression. Amikacin at this dose did not cause animal death, although it caused some changes in motor behaviors with a significant difference in comparison with control values. Gentamicin demonstrated significantly more potent effects on motor behaviors compared to the other AGs. Overall, the order of potency was gentamicin > dihydrostreptomycin > apramycin > amikacin. High doses of AGs could impair the skeletal muscle function and disrupt motor behaviors in mice. Furthermore, the paralytic potency of selected AGs on skeletal muscle was significantly different.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of nanocurcumin on fertility in murine model of polycystic ovary syndrome. 纳米姜黄素对多囊卵巢综合征小鼠模型生育力的影响

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2006604.3935

Zahra Aaly-Gharibeh, Mohammadreza Hosseinchi, Ali Shalizar-Jalali

{"title":"Effect of nanocurcumin on fertility in murine model of polycystic ovary syndrome.","authors":"Zahra Aaly-Gharibeh, Mohammadreza Hosseinchi, Ali Shalizar-Jalali","doi":"10.30466/vrf.2023.2006604.3935","DOIUrl":"10.30466/vrf.2023.2006604.3935","url":null,"abstract":"The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, in vitro fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. Fifty adult female mice were randomly categorized into five equal groups including non-treated control and PCOS (receiving 0.20 mg estradiol valerate (EV) intra-peritoneally once a day for 21 days) as well as NCC12.50 + PCOS, NCC25 + PCOS and NCC50 + PCOS (receiving respectively 12.50, 25.00 and 50.00 mg kg-1 NCC daily along with EV injection through oral gavages for 21 days) groups. Subsequently, ovarian histo-architecture and total anti-oxidant capacity, and malonaldehyde and catalase levels as well as in vitro fertilizing potential, early embryonic development and serum testosterone concentration were analyzed. Results showed that NCC in a dose-dependent manner improved ovarian cyto-architectural organization and oxidant/anti-oxidant balance along with IVF rate and pre-implantation embryo development in PCOS mice. These findings revealed that NCC at the doses of 25.00 and 50.00 mg kg-1 could alleviate PCOS-linked reproductive disruptions in female mice.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Hepatozoon felis infection in a domestic cat (Felis catus) in Iran. 伊朗首次报告家猫（Felis catus）感染猫肝吸虫。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2023.2005996.3928

Bijan Esmaeilnejad, Awat Samiei, Zahra Darabizade, Mohsen Akhoondi, Romina Salavati, Bahar Ahooghalandari, Vahid Fathipour, Arash Akhavan, Armin Fakheri

{"title":"First report of Hepatozoon felis infection in a domestic cat (Felis catus) in Iran.","authors":"Bijan Esmaeilnejad, Awat Samiei, Zahra Darabizade, Mohsen Akhoondi, Romina Salavati, Bahar Ahooghalandari, Vahid Fathipour, Arash Akhavan, Armin Fakheri","doi":"10.30466/vrf.2023.2005996.3928","DOIUrl":"10.30466/vrf.2023.2005996.3928","url":null,"abstract":"A 6-year-old male domestic short-haired cat (Felis catus) was presented with weakness, anorexia, fever, icterus, a painful abdomen, ruffled hair and a tick infestation, and it had no prior surgery. Laboratory analysis revealed left-shifted neutrophils, thrombocytopenia, low albumin content and high serum bilirubin concentration as well as activities of hepatic enzymes including alanine aminotransferase and aspartate aminotransferase. Azotemia and increased serum levels of creatinine and urea were also recorded. In Giemsa-stained blood smear, Hepatozoon gamonts were observed within neutrophils. Species-specific polymerase chain reaction assay was used to amplify an approximately 590 bp fragment of 18S rRNA gene and confirmed Hepatozoon felis infection. The cat was treated with imidocarb dipropionate and doxycycline and recovered completely. Six-month follow-up showed no recurrence. This study reveals the presence of H. felis in Iran and it should be considered in differential diagnosis in febrile and icteric cats. To the authors' knowledge, this is the first description of H. felis infection in a cat in Iran.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key molecules regulating the blood meals of Rhipicephalus sanguineus (Acari: Ixodidae) revealed by transcriptomics. 转录组学揭示了调控红腹角雉（Acari: Ixodidae）血餐的关键分子。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2011271.4007

Yajun Lu, Yijia Xu, Chenghang Yu, Shi Cheng, Qianfeng Xia, Zheng Bin

{"title":"Key molecules regulating the blood meals of Rhipicephalus sanguineus (Acari: Ixodidae) revealed by transcriptomics.","authors":"Yajun Lu, Yijia Xu, Chenghang Yu, Shi Cheng, Qianfeng Xia, Zheng Bin","doi":"10.30466/vrf.2024.2011271.4007","DOIUrl":"10.30466/vrf.2024.2011271.4007","url":null,"abstract":"Rhipicephalus sanguineus, a repulsive obligate blood feeder, is a three-host tick inflicting tremendous damage. Blood-sucking initiates tick-pathogen-host interactions along with alterations in the expression levels of numerous bioactive ingredients. Key molecules regulating blood meals were identified using the transcriptomic approach. A total number of 744 transcripts showed statistically significantly differential expression including 309 significantly upregulated transcripts and 435 significantly downregulated transcripts in semiengorged female ticks compared to unfed ticks, all collected in 2021. The top 10 differentially upregulated transcripts with explicit functional annotations included turripeptide OL55-like protein, valine tRNA ligase-like protein and ice-structuring glycoprotein-like protein. The top 10 differentially down-regulated transcripts were uncharacterized proteins. Gene Ontology (GO) enrichment analysis revealed four associated terms in the cellular component category and 16 in the molecular function category among the top 20 terms. Differentially expressed genes (DEGs) were enriched in GO terms ID 0000323 (lytic vacuole) and ID 0005773 (vacuole). The top 20 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included metabolism, cellular processes, organismal systems and human diseases. The DEGs were enriched in the KEGG term ID: ko-04142 (lysosome pathway) associated with intracellular digestion in the tick midgut epithelium. Molecular markers annotated via comparative transcriptomic profiling were expected to be candidate markers for the purpose of tick control.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of vitamin C and early-age thermal conditioning on pituitary adrenocorticotropic hormone cells in broilers chronically exposed to heat stress: an immunohistomorphometric and hormonal study. 维生素 C 和早期热调节对长期暴露于热应激的肉鸡垂体促肾上腺皮质激素细胞的影响：免疫组织形态学和激素研究。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2009320.3981

Jovana Čukuranović Kokoris, Zoran Ružić, Zdenko Kanački, Slobodan Stojanović, Smiljana Paraš, Verica Milošević

{"title":"Effects of vitamin C and early-age thermal conditioning on pituitary adrenocorticotropic hormone cells in broilers chronically exposed to heat stress: an immunohistomorphometric and hormonal study.","authors":"Jovana Čukuranović Kokoris, Zoran Ružić, Zdenko Kanački, Slobodan Stojanović, Smiljana Paraš, Verica Milošević","doi":"10.30466/vrf.2023.2009320.3981","DOIUrl":"10.30466/vrf.2023.2009320.3981","url":null,"abstract":"The aim of this study was to examine the effects of heat stress (HS) on the pituitary-adrenal axis and whether the treatments with early-age thermal conditioning (ETC) and vitamin C, alone and in combination, could have a beneficial effect in alleviating these effects. For the experiment, 400 one day-old broilers (both sexes) were used, being divided into four groups. The first group was the control (K), the second group (C) consisted of broilers which received vitamin C from the 22nd to the 42nd day via water in the amount of 2.00 g L-1, in the third group (T), broilers were exposed to ETC for a period of 24 hr at a temperature of 38.00 ± 1.00 ˚C and the fourth group (T + C) was the combination of T and C groups. Immunohistochemically positive adrenocorticotropic hormone (ACTH) cells of broilers in all groups were irregular or stellate and distributed in the periphery and central parts of the pituitary gland, as solitary cells or in clusters. In the T + C group of broilers, a significant increase in the area of ACTH cells (18.91%) and their cores (22.75%), and cortisol level in serum compared to the control group was observed. This reaction of broilers in the T + C group facilitated their adaptation to unfavorable consequences of HS. These results suggest that hypothalamic-pituitary-adrenal axis is stimulated after the exposure to chronic HS, enabling successful adaptation of broilers to adverse conditions.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of nanocurcumin on acetaminophen-induced hepatic and renal toxicities in pigeons. 纳米姜黄素对对乙酰氨基酚引起的鸽子肝肾毒性的保护作用

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2024.2011585.4009

Saeed Seifi, Nasser Vajdi, Shohreh Alian Samakkhah, Navideh Mirzakhani

{"title":"Protective effect of nanocurcumin on acetaminophen-induced hepatic and renal toxicities in pigeons.","authors":"Saeed Seifi, Nasser Vajdi, Shohreh Alian Samakkhah, Navideh Mirzakhani","doi":"10.30466/vrf.2024.2011585.4009","DOIUrl":"10.30466/vrf.2024.2011585.4009","url":null,"abstract":"In this study, the effects of nanocurcumin on acetaminophen-induced acute hepatorenal toxicity in domestic pigeons (Columba livia) were investigated. Fifteen pigeons were randomly assigned into three groups. Group I was served as a negative control group and received tap water as a placebo. Pigeons in groups II and III were administered acetaminophen at the beginning of the experiment (hr 0). Group III was further treated with nanocurcumin, at 12 hr after acetaminophen administration, being continued every 12 hr for two days. The birds were observed for clinical signs of acute drug toxicity. Blood samples were collected from the pigeons at hr 0, 12, 24 and 48 of the experiment for biochemical analysis of the serum. The results showed that acetaminophen toxicity increased the serum levels of aspartate aminotransferase, alanine aminotransferase, urea and uric acid in the pigeons. Nanocurcumin treatment of acetaminophen intoxicated pigeons attenuated increases in biomarkers of the liver and kidney functions towards control levels. Also, the consumption of nanocurcumin minimized histopathological changes in the liver and kidney. A mortality of 60.00% was seen in the acetaminophen-induced toxicity group; while, none of the birds treated with nanocurcumin died. It can be concluded that nanocurcumin alleviates the acetaminophen-induced acute toxic liver and kidney damages, which can lead to pigeon mortality.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-structural organization of the gallbladder mucous membrane of Anglo-Nubian goat. 盎格鲁努比亚山羊胆囊粘膜的超结构组织。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2024.2011081.4005

Anna Valerievna Prusakova, Nikolay Vyacheslavovich Zelenevskiy, Aleksey Viktorovich Prusakov, Anatoly Viktorovich Yashin, Vladimir Sergeevich Ponamarev

{"title":"Ultra-structural organization of the gallbladder mucous membrane of Anglo-Nubian goat.","authors":"Anna Valerievna Prusakova, Nikolay Vyacheslavovich Zelenevskiy, Aleksey Viktorovich Prusakov, Anatoly Viktorovich Yashin, Vladimir Sergeevich Ponamarev","doi":"10.30466/vrf.2024.2011081.4005","DOIUrl":"10.30466/vrf.2024.2011081.4005","url":null,"abstract":"Currently, studies devoted to establish the anatomical and histological patterns of the internal organs organization in animals depending on their species and breed, as well as conditions of detention are the most relevant. The liver morphology in representatives of the ruminant family has not been sufficiently studied. Questions regarding the micro- and ultra-structural organizations of the gallbladder wall remain open. The aim of the study was to establish the ultra-structural organization features of the gallbladder mucous membrane of an Anglo-Nubian goat. The material for the study was the gallbladder wall's fragments of an adult Anglo-Nubian goat. Further processing of the obtained samples was carried out to acquire histological preparations. Ultra-thin sections were photographed in a Jem-1011 electron microscope at magnifications of 2,500 - 3,000. It was found that the gallbladder mucous membrane of an Anglo-Nubian goat is formed by the epithelial layer and its own lamina. The epithelial layer is represented by a single-layer prismatic epithelium. The nuclei are light, with clear contours. The apical surface of epithelial cells forms microvilli and the cytoplasm of the apical pole of cells contains many electron-dense secretory granules. The lateral surfaces of the cells in their apical part are interconnected by tight contacts. The lamina propria is formed by loose connective tissue containing many blood vessels and nerve fibers. Referring to the scientific literature describing possible pathologies of the gallbladder, we can conclude that the picture presented in the results generally corresponds to the position of the gallbladder without pathologies.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran. 用分离、血清学和分子方法筛查伊朗东阿塞拜疆省的马勒氏伯克霍尔德氏菌。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2024.2010651.4002

Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi

{"title":"Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran.","authors":"Hasan Tizfahm Tikmehdash, Alireza Dehnad, Nader Mosavari, Behroz Naghili Hokmabadi, Sanaz Mahmazi","doi":"10.30466/vrf.2024.2010651.4002","DOIUrl":"10.30466/vrf.2024.2010651.4002","url":null,"abstract":"Glanders caused by Burkholderia mallei is one of the most dangerous zoonotic diseases in solipeds. Clinical diagnosis of this disease in its early stages in horses, is difficult. This study investigated serological and molecular identification of B. mallei in East Azerbaijan province. In the third and fourth quarters of 2020, throughout 2021, and in the first and second quarters of 2022, the complement fixation test (CFT) was performed on 350 horses. The malleination was used to confirm the positive CFT cases. Blood samples were taken for culture and for preparing serums to perform the enzyme-linked immunosorbent assay (ELISA). Deep eye discharge, nostril, cutaneous ulcers and lymph fluid swabs were cultured, and polymerase chain reaction (PCR) was carried out. Eleven horses were CFT-positive. Based on the malleination on the 11 horses, six were affected by glanders, five were not affected (false positive), and one horse was CFT-negative despite exhibiting clinical signs. It was positive by malleination, ELISA and PCR. A total number of seven positive cases of glanders were diagnosed. The B. mallei could not be isolated, but the Burkholderia cepacia complex was isolated in one case. Except for three cases, the results of the CFT, mallein and ELISA tests were consistent. The amount of confidence interval was 95.00%. It is suggested that ELISA could be used as a complement to CFT in screening and, if positive results are observed in one of the tests, the entire herd must be examined more accurately using the mallein and western blot confirmatory tests.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt. 从埃及驼奶中分离出的金黄色葡萄球菌抗菌药耐药性基因的分子特征。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2023.2007392.3946

Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey

{"title":"Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt.","authors":"Mohamed Mohamed Ali, Salwa Mahmoud Helmy, Hanan Ali Fahmy, Haitham Elaadli, Ibrahim Elsayed Eldesoukey","doi":"10.30466/vrf.2023.2007392.3946","DOIUrl":"10.30466/vrf.2023.2007392.3946","url":null,"abstract":"Staphylococcus aureus is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of S. aureus in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for S. aureus using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of S. aureus isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for S. aureus. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (mecA), β-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDRS aureus as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant S. aureus isolates.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital. 西班牙一家兽医教学医院从环境和兽医人员中分离出的葡萄球菌的流行率和抗微生物耐药性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2016107.4086

Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez

{"title":"Prevalence and anti-microbial resistance of Staphylococcus spp. isolated from the environment and veterinary personnel in a Spanish veterinary teaching hospital.","authors":"Ana-María Ríos, Silvia Penelo, María-Rosario Barquero, Tania Ayllón, Gustavo Ortiz-Díez","doi":"10.30466/vrf.2024.2016107.4086","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016107.4086","url":null,"abstract":"Methicillin-resistant Staphylococcus (MRS) bacteria, including methicillin-resistant S. aureus and methicillin-resistant S. pseudintermedius (MRSP), pose a significant threat in veterinary medicine, given their potential for zoonotic transmission and their implications for companion animals and humans' health. This study aimed to assess the prevalence of MRS and anti-microbial resistance patterns at a university clinical hospital in Madrid, Spain. Samples were collected from both the environment and hospital staff at Veterinary Clinical Hospital of Alfonso X el Sabio University. Anti-microbial susceptibility assays, molecular detection of mecA gene and genetic relationships among the identified bacterial strains were performed. The study revealed an MRS prevalence of 1.50% in environmental samples, with MRSP accounting for 0.75% of the cases. Genetically related MRSP strains were found in different hospital areas. Among hospital staff, there was a MRS prevalence of 14.03%, including S. pseudintermedius and S. epidermidis strains. Antibiogram tests revealed multi-drug resistance among MRSP strains. Additionally, methicillin-resistant coagulase-negative staphylococci were isolated, suggesting potential cross-species transmission. This study underscores the presence of MRS in a veterinary clinical hospital, highlighting the significance of infection control through the implementation of protective measures, stringent hygiene practices among personnel and in the environment and responsible use of antibiotics. Further research is necessary to assess MRS incidence in animal patients and explore geographical variations, enhancing our understanding of MRS in veterinary medicine and addressing its challenges.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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