Veterinary Research Forum最新文献

Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry. 伊朗卡尚地区肉鸡4型禽腺病毒的分子和组织病理学特征：对家禽业疾病控制和预防的意义

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030590.4302

Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi

{"title":"Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry.","authors":"Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi","doi":"10.30466/vrf.2024.2030590.4302","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030590.4302","url":null,"abstract":"Fowl adenovirus (FAdV) is a DNA virus causing significant diseases, like inclusion body hepatitis, hydropericardium-hepatitis syndrome (HHS), and gizzard erosion. These diseases lead to severe economic losses in the poultry industry. Recent increases in HHS outbreaks in Iran, particularly among broilers, prompted this study to analyze FAdV isolates in Kashan, Iran. In December 2021, a high-mortality HHS outbreak in a Kashan broiler flock led to liver and heart samples being sent for analysis. Histopathological investigations revealed mononuclear hepatitis and intra-nuclear viral inclusion bodies in hepatocytes. Polymerase chain reaction and phylogenetic analyses confirmed the presence of FAdV-4 (accession number: PP856395), showing 99.99% identity with strains from Japan, the United Arab Emirates, Pakistan, and the United States. These findings highlight the genetic similarity and potential common origin of FAdV-4 strains. This study emphasizes the need for heightened biosecurity measures and effective vaccination strategies to mitigate the spread of FAdV-4. The confirmed presence of FAdV-4 in central Iran poses a significant threat to the poultry industry, necessitating prompt action to prevent substantial economic losses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"19-25"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine. 黑腿病疫苗抗体检测用邵氏梭状芽胞杆菌细胞表面蛋白的纯化方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2023812.4190

Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri

{"title":"A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine.","authors":"Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri","doi":"10.30466/vrf.2024.2023812.4190","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023812.4190","url":null,"abstract":"Cell-surface proteins of Clostridium chauvoei were purified using a simple method. Bacterial cultures were centrifuged and agitated vigorously in phosphate buffered saline with or without further glycine treatment and ammonium sulfate precipitation. Rabbits were immunized subcutaneously with a blackleg disease vaccine twice with a two-week interval. Immunized sera were collected one week after the second injection. Enzyme-linked immunosorbent assay (ELISA) was performed using the proteins purified by the second method as the coating antigen. Bradford assay results showed a higher protein concentration in the second than the first method. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis analysis showed multiple bands for the cell-surface proteins of C. chauvoei in the first method and a sharp band equivalent to flagellin protein in the second method. The ELISA results indicated that the purified proteins were capable of detecting antibodies against Blackleg disease vaccine. The purified protein would be an alternative antigen for indirect ELISA in order to monitor the immune response in vaccinated farm animals.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"57-61"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats. 百里醌通过抗氧化和抗炎机制减轻大鼠爪切引起的自发性和诱发性疼痛。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2025053.4204

Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh

{"title":"Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats.","authors":"Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh","doi":"10.30466/vrf.2024.2025053.4204","DOIUrl":"10.30466/vrf.2024.2025053.4204","url":null,"abstract":"Surgery commonly causes post-operative pain that should be alleviated to prevent complications. In addition to the use of synthetic drugs, there has been a widespread desire to use medicinal plants for surgical pain management. Thymoquinone (TQ), a constituent of Nigella sativa black seeds, exhibits a potent anti-oxidant property. Celecoxib (CLX), a potent non-steroidal anti-inflammatory drug, is widely used in pain management. In the present study, the effects of TQ and CLX on pain caused by hind paw surgical incision were compared. Fifty-six rats were divided into four groups of 14 rats as intact, vehicle, TQ, and CLX groups. In each group, six rats were planned to record pain-related behaviors on days 1 - 10 and eight rats were designed for determination of serum biochemical alterations on days 1 (four rats) and 3 (four rats) after surgery. Oral administrations of TQ and CLX at a same dose of 10.00 mg kg-1 alleviated paw lifting number (spontaneous pain) and paw withdrawal threshold evoked by von Frey filaments on metal mesh floor, improved the decreased contents of serum total anti-oxidant capacity and superoxide dismutase, and restored the increased levels of serum malondialdehyde and tumor necrosis factor-alpha. The results suggested that TQ by employing anti-oxidant and anti-inflammatory mechanisms, might relieve the pain induced by hind paw plantar incision, being comparable with CLX.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"89-96"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture. 聚β-羟基丁酸对鲤鱼鱼种的促生长潜力及免疫刺激作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2024674.4200

Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz

{"title":"Growth-promoting potential and immunostimulatory of poly-β-hydroxybutyrate in common carp (Cyprinus carpio) fingerlings culture.","authors":"Rabeeh Ziaei, Hossein Ouraji, Ebrahim Najdegerami, Reza Akrami, Hossein Chitsaz","doi":"10.30466/vrf.2024.2024674.4200","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024674.4200","url":null,"abstract":"The natural polymer poly-β-hydroxybutyrate (PHB) is converted to β-hydroxybutyric acid, which is similar to short-chain fatty acids, via microbial fermentation and host enzyme breakdown. This study investigated the impact of different PHB concentrations (Control, 1.00, 3.00, and 5.00% substitution) on growth performances and fish welfare in common carp fingerlings. After a 60-day trial, fish fed on diet containing 1.00% PHB exhibited significantly higher weight gain and improved feed conversion efficiency compared to the control group. Furthermore, analysis of enzymatic activity showed elevated levels of total protease and amylase in PHB-fed treatments compared to the control. Red blood cell counts, hemoglobin, and hematocrit levels remained unaffected and a significant increase in white blood cell count was observed in fish fed on diets containing 1.00 and 3.00% PHB compared to the control group. Furthermore, fish fed on diets containing 1.00 and 3.00% PHB demonstrated significantly higher total protein levels and lower glucose concentrations as well as reduced hepatic enzyme activities (aspartate aminotransferase and alanine aminotransferase) compared to both the control and 5.00% PHB groups. Assessment of antioxidant and immune parameters revealed significantly increased complement hemolytic activity and immunoglobulin M levels coupled with decreased malondialdehyde concentrations in the plasma of PHB-fed fish compared to the control group. In conclusion, dietary supplementation with PHB, especially at the 1.00% level, enhanced growth performance and improved nutritional and health indicators in the fingerlings. These findings suggested that PHB had the potential to be a valuable dietary additive for this species.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"43-50"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis. 在类风湿性关节炎实验模型中将胡椒碱与泼尼松龙结合使用的益处

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2031860.4316

Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid

{"title":"Benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis.","authors":"Asghar Morvaridi, Seyyed Meysam Abtahi Froushani, Amir Abbas Farshid","doi":"10.30466/vrf.2024.2031860.4316","DOIUrl":"10.30466/vrf.2024.2031860.4316","url":null,"abstract":"This study evaluated the impact of combining piperine and prednisolone on clinical symptoms and immune responses in Wistar rats with rheumatoid arthritis (RA) induced by Freund's complete adjuvant due to piperine known anti-inflammatory and immunomodulatory properties. The RA rats were randomly divided into five groups (n = 10): The RA rats were treated with phosphate-buffered saline, RA rats treated with piperine (100 mg kg-1 orally), RA rats treated with prednisolone (10.00 mg kg-1 orally), and RA rats treated with a combination of piperine and prednisolone (half doses of each orally). Treatment started on day five post-induction when all rats had a clinical score of ≥ 1. Disease symptoms were monitored every other day until day 23 post-induction. Combining the two medications at half doses led to a more significant reduction in disease severity, weight improvement, and histopathological changes compared to using each drug alone at the full doses. The combined treatment group exhibited the most favorable response in C-reactive protein, myeloperoxidase, and nitric oxide biochemical tests compared to the other treatment groups. The combined treatment group showed decreased expression of T-bet and RORɣt genes. However, there was no statistically significant difference in the expression of Foxp3 and GATA3 genes compared to the group receiving prednisolone alone. Overall, combining piperine with prednisolone may prove to be a beneficial approach for managing RA.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"117-124"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bioactive peptides on heat stress-induced testiculopathies in mature rats: immunohistopathological evidence. 生物活性肽对成熟大鼠热应激性睾丸病变的影响：免疫组织病理学证据。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2032033.4318

Mahsa Valizadeh, Vahid Nejati, Ali Shalizar-Jalali, Ebrahim Najdegerami, Gholamreza Najafi

{"title":"Effect of bioactive peptides on heat stress-induced testiculopathies in mature rats: immunohistopathological evidence.","authors":"Mahsa Valizadeh, Vahid Nejati, Ali Shalizar-Jalali, Ebrahim Najdegerami, Gholamreza Najafi","doi":"10.30466/vrf.2024.2032033.4318","DOIUrl":"10.30466/vrf.2024.2032033.4318","url":null,"abstract":"Exposure to extreme temperature conditions such as occurs in certain occupations is known to induce male infertility. In humans and most of the mammals, it has been shown that whole-body heat stress (HS) decreases fertility and produces defective embryos. Hence, the present study aimed at gaining some insights into the mechanisms producing defects after HS. In the present study, 56 mature male Wistar rats were randomly categorized into eight groups (n = 7), including group 1: Control, groups 2: Bioactive peptides (BPs; 10.00 mg kg-1), groups 3, 4, and 5: Heat-stressed (37.00, 39.00, and 43.00 ˚C for 20 min, respectively), and groups 6, 7, and 8: Heat-stressed along with BPs (10.00 mg kg-1), respectively. All treatments were administered orally once per day. The HS was induced through the immersion of rat scrotums in a water bath. After 45 days, rats were sacrificed and left testes were removed, fixed, and used for histological and immunohistochemical studies. Harvested right testes were also used for oxidative stress assessments and molecular analyses. Heat stress increased testicular tissue damage, elevated oxidative stress and reactive oxygen species production, and increased germ cells apoptosis, p53 and caspase 3 expressions, and Bax/Bcl-2 ratio. Treatment with BPs as a substance with anti-oxidant properties ameliorated the damage caused by HS. The results of this study highlight the protective role of BPs in the reproductive tract under HS. Bioactive peptides may have potential function against testicular tissue oxidative stress and apoptosis.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"106-116"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective effect of royal jelly on dibutyl phthalate-induced liver injury in rats. 蜂王浆对邻苯二甲酸二丁酯所致大鼠肝损伤的保护作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2033878.4338

Mahdieh Nezami Majd, Goudarz Sadeghi-Hashjin, Hassan Malekinejad, Ali Rassouli

{"title":"Hepatoprotective effect of royal jelly on dibutyl phthalate-induced liver injury in rats.","authors":"Mahdieh Nezami Majd, Goudarz Sadeghi-Hashjin, Hassan Malekinejad, Ali Rassouli","doi":"10.30466/vrf.2024.2033878.4338","DOIUrl":"10.30466/vrf.2024.2033878.4338","url":null,"abstract":"Phthalate esters, such as dibutyl phthalate (DBP), are extensively utilized and human and animal exposure leads to serious toxic effects, including hepatotoxicity. In the present study the protective effects of royal jelly (RJ) on DBP-induced liver damage was investigated. A total number of 40 Wistar albino rats were randomly divided into eight groups (n = 5): control (corn oil), DBP (500 mg kg-1), RJ (200 mg kg1), Quercetin (QCN; 50.00 mg kg-1), RJ (100 mg kg-1) + DBP, RJ (200 mg kg-1) + DBP, RJ (300 mg kg-1) + DBP, QCN (50.00 mg kg-1) + DBP. After 28 days of daily oral gavage treatment, animals were euthanized. The insulin resistance index, lipid profile and hepatic enzymes were measured on the collected serum samples. Moreover, oxidative and nitrosative stress biomarkers were determined in the liver. Histopathological alterations and ultimately cytochrome P450 2E1 (CYP2E1) activity was also assessed. Data obtained revealed that RJ significantly reduced the insulin resistance index and liver enzymes level in RJ-DBP groups. At the same time, RJ recovered the DBP-induced oxidative stress and restored the DBP-depleted glutathione. Moreover, RJ improved lipid profile and reduced significantly the DBP-induced hepatic CYP 2E1 activity in RJ-DBP groups. Dibutyl phthalate induced-hepatic damage such as necrosis of hepatocytes and scattered bleeding was alleviated in RJ-DBP group. Our data suggested that the administration of RJ could protect the DBP-induced hepatic functional and structural alterations. The RJ protective effects might be attributed to its antioxidant and anti-inflammatory properties and reduced CYP 2E1 activity.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"97-105"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture. 胫骨平台平截骨：基质金属蛋白酶在颅十字韧带断裂后犬膝关节稳定长期监测中的意义。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2023.2017099.4089

Dragan Jevrem Ristanović, Jelena Vladimir Francuski Andrić, Milena Danilo Radaković, Kristina Adam Spariosu, Ivan Željko Vlahek, Marija Tomo Lipar, Petar Sreten Milosavljević, Mario Ivan Kreszinger

{"title":"Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture.","authors":"Dragan Jevrem Ristanović, Jelena Vladimir Francuski Andrić, Milena Danilo Radaković, Kristina Adam Spariosu, Ivan Željko Vlahek, Marija Tomo Lipar, Petar Sreten Milosavljević, Mario Ivan Kreszinger","doi":"10.30466/vrf.2023.2017099.4089","DOIUrl":"10.30466/vrf.2023.2017099.4089","url":null,"abstract":"Cranial cruciate ligament rupture is one of the most common causes of osteoarthritis in dogs. Surgical stabilization is obligatory and tibial plateau levelling osteotomy (TPLO) is the most commonly used surgical technique. Studies on the long-term monitoring of matrix metallo-proteinases (MMPs) and acute-phase proteins are limited, especially those with parallel monitoring of changes in the serum, synovial fluid (SF) and urine. We aimed to describe long-term changes in 1) MMPs: gelatinases and caseinases, 2) APPs: ceruloplasmin, haptoglobin and paraoxonase-1, and 3) the correlation of MMPs in the serum, SF and urine with lameness in dogs 2 and 6 months undergoing TPLO. From 17 dogs diagnosed with cranial cruciate ligament rupture, sera, SF and urine samples were collected preoperative 2 and 6 months after the surgery. Relative activity (RA) of MMPs was measured in all samples using zymography. Acute-phase proteins were measured in the serum and SF using spectrophotometry and agarose gel electrophoresis. Relative activity of MMPs in serum was not change at different sampling points. In SF, a marked decrease in MMPs RA was evident, however, only RA of caseinases was significantly reduced during the recovery period. In urine, RA of caseinases was positively correlated with the lameness score with decreasing activity trend during time. Serum and synovial acute-phase proteins were not changed after surgery, which was consistent with the agarose gel analyses. Decreased activity of caseinases pointed to reduced degradation of extracellular matrix after TPLO. Synovial and urine caseinases are potential biomarkers in predicting the recovery outcome following stifle stabilization.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"63-69"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of olive oil against cardiac aging through mitophagy and apoptosis. 橄榄油通过有丝分裂和细胞凋亡对心脏衰老的保护作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030624.4304

Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari

{"title":"Protective effects of olive oil against cardiac aging through mitophagy and apoptosis.","authors":"Siamak Shahidi, Khadijeh Ramezani-Aliakbari, Abdolrahman Sarihi, Ali Heshmati, Elham Shiri, Shiva Nosrati, Sayed Payam Hashemi, Mitra Bahrami, Fatemeh Ramezani-Aliakbari","doi":"10.30466/vrf.2024.2030624.4304","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030624.4304","url":null,"abstract":"Cardiac mitochondrial dysfunction is an important feature of aged heart. However, there is still no potent agent to ameliorate cardiac function abnormalities in aged hosts. Olive oil (OLO), containing monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-diabetic, anti-inflammatory, and anti-hypertensive effects. We evaluated the beneficial impacts of OLO against aging-related cardiac dysfunction. Wistar rats were randomly allotted into three groups with eight rats, including control, aged rats receiving D-galactose (D-GAL), and aged rats administrated with D-galactose plus OLO (D-GAL + OLO). Aged animals were received D-GAL at a dose of 150.00 mg kg-1 daily through intra-peritoneal injection for aging induction. The animals in D-GAL + OLO group were co-administrated with oral OLO at a dose of 1.00 mL kg-1 by gavage feeding daily. The administration term was eight weeks. A histological examination of heart tissue was performed. The heart tissues were also harvested to assay the oxidative stress and molecular parameters. The aged animals showed cardiac hypertrophy, increased malondialdehyde level and Bax expression, and reduced mitofusin 2, phosphatase and tensin homologue-induced putative kinase 1, dynamin-related protein 1, and Bcl2 expressions in comparison with the control animals. The OLO treatment ameliorated all these parameters. Overall, OLO could improve cardiac aging through reducing oxidative stress, enhancing genes mediated mitophagy, and improving genes mediated apoptosis in the heart.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"27-33"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases. N- 乙酰半胱氨酸在治疗犬血液原虫病引起的全血细胞减少症中可增强骨髓活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2033155.4328

Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das

{"title":"N-acetylcysteine enhances bone marrow activity in treating pancytopenia induced by canine hemoprotozoan diseases.","authors":"Narayani Yadav, Debabrata Mondal, Raguvaran Raja, Ensha Lomiya Ma, Karam Pal Singh, Dushyant Kumar Sharma, Arvind Kumar Das","doi":"10.30466/vrf.2024.2033155.4328","DOIUrl":"https://doi.org/10.30466/vrf.2024.2033155.4328","url":null,"abstract":"Canine hemoprotozoan diseases viz. ehrlichiosis and babesiosis are mostly associated with critical anemia and thrombocytopenia with pancytopenic changes, leading to multi-organ failure. For faster recovery of patients with complicated hemoprotozoan diseases, whole blood transfusion or bone marrow stimulating agents to produce more red blood cells (RBCs) and platelets might be helpful. Unfortunately, canine specific transfusion procedures are expensive and even not available in many developing countries. Development of alternate therapeutic modality by bone marrow stimulation to augment the production of RBCs and platelets and thus, to treat the critical pancytopenic patients is and an urgent necessity. N-acetylcysteine (NAC), acts as a precursor of reduced glutathione and increases the production of bone marrow B cells. It also improves viability and self-renewal capacity of stem cells and thus, boosts hematopoietic differentiation by protecting induced pluripotent stem cells. This study envisaged to develop alternate therapeutic approach to combat pancytopenia secondary to canine hemoprotozoan diseases. Bone marrow mediated aplastic pancytopenia was induced experimentally by administration of cyclophosphamide in rats. Bone marrow stimulating property of NAC was compared with desmopressin, another bone marrow stimulator, which revealed better in terms of hematobiochemical and histopathological changes. Results of rat model study were extrapolated in clinical canine hemoprotozoan cases having pancytopenia. Dogs treated with hemoprotozoan disease specific therapy along with NAC rendered favorable changes by haltering the progression of critical anemia and thrombocytopenia. Study revealed that supplementation of NAC along with canine hemoprotozoan specific therapy is beneficial to alleviate pancytopenia.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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