Veterinary Research Forum最新文献

Evaluation of safety and humoral immunogenicity of inactivated Newcastle disease virus genotype VII entrapped in calcium phosphate nanoparticles in chickens. 磷酸钙纳米颗粒包埋灭活的基因型新城疫病毒在鸡体内的安全性和体液免疫原性评价。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-12-15 DOI: 10.30466/vrf.2025.2044018.4507

Bita Darabi-Aref, Tara Emami, Mohammad Abdoshah, Mohsen Mahmoudzadeh Akhijahani, Leila Pishraft-Sabet

{"title":"Evaluation of safety and humoral immunogenicity of inactivated Newcastle disease virus genotype VII entrapped in calcium phosphate nanoparticles in chickens.","authors":"Bita Darabi-Aref, Tara Emami, Mohammad Abdoshah, Mohsen Mahmoudzadeh Akhijahani, Leila Pishraft-Sabet","doi":"10.30466/vrf.2025.2044018.4507","DOIUrl":"10.30466/vrf.2025.2044018.4507","url":null,"abstract":"Newcastle disease (ND) is a highly contagious and severe disease that affects birds, including domestic poultry, causing significant economic losses to the poultry industry. The disease is caused by the ND virus (NDV). Despite extensive vaccination efforts against NDV, controlling the disease remains challenging, primarily due to the emergence of new genotypes. Recent attention has focused on calcium phosphate nanoparticles (CaP NPs) as promising adjuvants for vaccines. This study aimed to design and construct CaP NPs containing inactivated NDV genotype VII and evaluate their safety and humoral immunogenicity in chickens. Following virus propagation in specific-pathogen-free eggs and inactivation with formalin, CaP NPs containing inactivated NDV were prepared using in situ and adsorption methods. The NPs were characterized for shape, size, polydispersity index and surface charge. Chickens were immunized subcutaneously with CaP NPs containing inactivated NDV and the humoral immune response against NDV was assessed using HI and enzyme-linked immunosorbent assays. Results showed a significant increase in the mean antibody titer against NDV in chickens treated with both NP structures compared to control groups. No significant difference was observed between the two NP preparation methods. The safety of CaP NPs containing inactivated NDV was confirmed. However, mucosal immunization with these NPs did not yield satisfactory results indicating the need for further research. Overall, this study confirmed the positive role of CaP as an adjuvant in enhancing the humoral immune response against NDV via injection and highlighted the need for continued research on mucosal immunization with the NPs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 12","pages":"699-706"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12854165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammary comedocarcinoma in a rabbit. 兔乳腺粉刺癌。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-12-15 DOI: 10.30466/vrf.2025.2049185.4610

Ghasem Farjanikish, Saman Salmani, Mohammadhossein Abbasi, Mohammadreza Bayat, Erfan Vali

引用次数: 0

First case of serpentovirus infection in a ball python (Python regius) in Thailand: a case report with molecular characterization. 泰国第一例蛇状病毒感染球蟒（蟒蛇）：一例分子特征的病例报告。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2034234.4345

Panithi Laoungbua, Nararat Laopichienpong, Apinya Longya, Lawan Chanhome, Taksa Vasaruchapong, Tanapong Tawan, Narongsak Chaiyabutr

{"title":"First case of serpentovirus infection in a ball python (Python regius) in Thailand: a case report with molecular characterization.","authors":"Panithi Laoungbua, Nararat Laopichienpong, Apinya Longya, Lawan Chanhome, Taksa Vasaruchapong, Tanapong Tawan, Narongsak Chaiyabutr","doi":"10.30466/vrf.2024.2034234.4345","DOIUrl":"10.30466/vrf.2024.2034234.4345","url":null,"abstract":"The pet snake industry in Thailand has seen a significant rise in popularity, with the ball python (Python regius) becoming a frequently kept species. However, respiratory disease poses a notable health concern, and various viral pathogens, including serpentoviruses (formerly classified as nidoviruses), have been implicated. While serpentovirus infections have been reported globally in diverse snake species, no documented cases had previously been identified in Thailand. This case report describes a 9-month-old ball python presenting to the Reptile Science Clinic at the Queen Saovabha Memorial Institute in Bangkok, Thailand, with respiratory distress and emaciation. Despite veterinary intervention, the snake succumbed to the infection within two weeks. Post-mortem examination revealed marked mucus accumulation within the oral cavity and necrotic oral mucosa. Histopathological analysis demonstrated severe catarrhal pneumonia. Molecular investigations confirmed the presence of serpentovirus in the lung tissue of the affected python, with subsequent sequence analysis revealing close homology to known serpentoviruses in ball pythons. This report documents the first confirmed case of serpentovirus infection in a pet snake in Thailand.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"361-364"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of melatonin loaded chitosan hydrogel on rat spinal cord injury. 褪黑素负载壳聚糖水凝胶对大鼠脊髓损伤的影响。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-09-15 DOI: 10.30466/vrf.2025.2043413.4497

Fariborz Afroozi, Ahmad Asghari, Gholamreza Abedi, Pejman Mortazavi, Hesam Uddin Hoseinzadeh

{"title":"Effect of melatonin loaded chitosan hydrogel on rat spinal cord injury.","authors":"Fariborz Afroozi, Ahmad Asghari, Gholamreza Abedi, Pejman Mortazavi, Hesam Uddin Hoseinzadeh","doi":"10.30466/vrf.2025.2043413.4497","DOIUrl":"10.30466/vrf.2025.2043413.4497","url":null,"abstract":"Spinal cord injury (SCI) results in the demise of neural and glial cells, as well as extensive neuro- inflammation. Hydrogel formulation for prolonged release of melatonin (Mel) has demonstrated enhanced effectiveness and safety. In this study, SCI was induced in rats by contusion at the T9 vertebrae. Chitosan (CH) /Mel hydrogel was fabricated and characterized using scanning electron microscopy (SEM) and Fourier transform infra-red to examine its specific effects on the apoptotic and histopathological markers of SCI. The scanning electron microscopy images revealed the presence of porosity in the CH/Mel hydrogel. Forty male Wistar rats were randomly divided into five groups (n = 8), including sham, control (SCI-induced treated locally with 100 µL CH hydrogel), and groups 3, 4, and 5 (treated locally immediately after SCI induction with 100 µL CH hydrogel containing 50.00, 100, and 200 mg kg-1 Mel, respectively). The CH/Mel hydrogel at a dose of 25.00 mg mL-1 significantly increased cell viability in the U87 cell line after 24 hr of exposure. However, at 48 and 72 hr after exposure, Bax and Bcl2 expressions were significantly increased and reduced in the SCI group, respectively, and CH/Mel hydrogel could alleviate their expressions, especially in higher doses. In addition, S100 protein expression was up-regulated in the SCI group. However, CH/Mel hydrogel down-regulated it in a dose-dependent manner. The histopathological findings demonstrated that CH/Mel hydrogel dramatically improved SCI outcomes, like vacuolar degeneration, necrosis, and severe cystic and axonal degenerations. In conclusion, CH/Mel hydrogel induced neuroprotection and it had the potential to be used as a therapeutic agent for the treatment of SCI.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 9","pages":"537-543"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12584892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145453412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical evidence of lipid peroxidation role in diethylnitrosamine-induced hepatocellular carcinoma in male Wistar albino rats. 脂质过氧化作用在雄性Wistar白化大鼠二乙基亚硝胺诱导的肝细胞癌中的免疫组织化学证据。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2036652.4378

Emin Karakurt, Serpil Dag, Hilmi Nuhoglu, Ayfer Yıldız, Emre Kurtbas

{"title":"Immunohistochemical evidence of lipid peroxidation role in diethylnitrosamine-induced hepatocellular carcinoma in male Wistar albino rats.","authors":"Emin Karakurt, Serpil Dag, Hilmi Nuhoglu, Ayfer Yıldız, Emre Kurtbas","doi":"10.30466/vrf.2024.2036652.4378","DOIUrl":"10.30466/vrf.2024.2036652.4378","url":null,"abstract":"This study aimed to evaluate malondialdehyde (MDA) expressions using the immunohisto-chemical method in order to reveal the role of lipid peroxidation in the development and progression of hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN) in male Wistar albino rats. Avidin-biotin-peroxidase method was used for immunohistochemical evaluation. Histopathological examinations revealed that DEN caused a mixed pattern (trabecular and acinar formations) of HCC in the majority of rats. The MDA positive stainings were significantly increased in rats in the HCC group compared to the healthy rats in the control group. In conclusion, this study data contain three important findings. The first one is that DEN triggers the formation of reactive oxygen species (ROS), excessively produced ROS cause oxidative stress, and as a result, oxidative stress strongly causes lipid peroxidation. Secondly, it is clear that there is an important relationship between oxidative stress-induced lipid peroxidation and HCC progression. At the same time, MDA is an useful biomarker in determining the prognosis of patients with HCC. The third and final finding is that intra-peritoneal DEN injection once a week for 20 weeks, but not in combination with other promoting chemical agents, appears to be very effective in inducing experimental HCC.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"375-380"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological changes in fibrous tissue of rat myocardium after administration of dispersed allogeneic biomaterial. 分散异体生物材料对大鼠心肌纤维组织形态学的影响。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2035761.4367

Lebedeva Anna Ivanovna, Musina Lyalya Akhiyarovna, Prusakov Alexey Viktorovich, Vladimir Sergeevich Ponamarev

{"title":"Morphological changes in fibrous tissue of rat myocardium after administration of dispersed allogeneic biomaterial.","authors":"Lebedeva Anna Ivanovna, Musina Lyalya Akhiyarovna, Prusakov Alexey Viktorovich, Vladimir Sergeevich Ponamarev","doi":"10.30466/vrf.2024.2035761.4367","DOIUrl":"10.30466/vrf.2024.2035761.4367","url":null,"abstract":"To improve the structure of the heart muscle after myocardial infarction, methods of regenerative medicine are used. One of the promising areas is the intra-myocardial administration of acellular allogeneic biomaterial (AB). The AB stimulates the regeneration of organs and tissues. But, the effect of AB on the myocardium after its fibrous ischemic degeneration has not been assessed. The aim of the study was to assess the morphological structure of the heart after cryodestruction in the late period and the use of AB. Chronic myocardial infarction was modeled in 80 male rats. To simulate chronic myocardial infarction and fibrosis formation, contact cryodestruction was performed. After 45 days, during repeated thoracotomy in the main group, AB suspension was injected into the area of the cryogenic myocardial scar. Six injections of 0.50 mg of dry substance were administered. In the control group, physiological solution was injected. After AB administration, following 7, 14, 30, and 45 days, the animals were withdrawn from the experiment, and the hearts were excised for histological and immunohistochemical studies. The AB underwent gradual phagocytosis by macrophages and gradually replaced by loose fibrous connective tissue with the presence of cardiac troponin I+ labeled muscle cells, which over time underwent hypertrophy. Cardiomyocytes were grouped in the AB implantation zone as separate clusters. The heart mass did not change in both experimental groups. The use of AB in the area of the formed cryogenic myocardial scar promoted the transformation of dense fibrous connective tissue into loose tissue and its replacement with cardiac muscle tissue.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"381-386"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry. 伊朗卡尚地区肉鸡4型禽腺病毒的分子和组织病理学特征：对家禽业疾病控制和预防的意义

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030590.4302

Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi

{"title":"Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry.","authors":"Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi","doi":"10.30466/vrf.2024.2030590.4302","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030590.4302","url":null,"abstract":"Fowl adenovirus (FAdV) is a DNA virus causing significant diseases, like inclusion body hepatitis, hydropericardium-hepatitis syndrome (HHS), and gizzard erosion. These diseases lead to severe economic losses in the poultry industry. Recent increases in HHS outbreaks in Iran, particularly among broilers, prompted this study to analyze FAdV isolates in Kashan, Iran. In December 2021, a high-mortality HHS outbreak in a Kashan broiler flock led to liver and heart samples being sent for analysis. Histopathological investigations revealed mononuclear hepatitis and intra-nuclear viral inclusion bodies in hepatocytes. Polymerase chain reaction and phylogenetic analyses confirmed the presence of FAdV-4 (accession number: PP856395), showing 99.99% identity with strains from Japan, the United Arab Emirates, Pakistan, and the United States. These findings highlight the genetic similarity and potential common origin of FAdV-4 strains. This study emphasizes the need for heightened biosecurity measures and effective vaccination strategies to mitigate the spread of FAdV-4. The confirmed presence of FAdV-4 in central Iran poses a significant threat to the poultry industry, necessitating prompt action to prevent substantial economic losses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"19-25"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine. 黑腿病疫苗抗体检测用邵氏梭状芽胞杆菌细胞表面蛋白的纯化方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2023812.4190

Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri

{"title":"A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine.","authors":"Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri","doi":"10.30466/vrf.2024.2023812.4190","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023812.4190","url":null,"abstract":"Cell-surface proteins of Clostridium chauvoei were purified using a simple method. Bacterial cultures were centrifuged and agitated vigorously in phosphate buffered saline with or without further glycine treatment and ammonium sulfate precipitation. Rabbits were immunized subcutaneously with a blackleg disease vaccine twice with a two-week interval. Immunized sera were collected one week after the second injection. Enzyme-linked immunosorbent assay (ELISA) was performed using the proteins purified by the second method as the coating antigen. Bradford assay results showed a higher protein concentration in the second than the first method. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis analysis showed multiple bands for the cell-surface proteins of C. chauvoei in the first method and a sharp band equivalent to flagellin protein in the second method. The ELISA results indicated that the purified proteins were capable of detecting antibodies against Blackleg disease vaccine. The purified protein would be an alternative antigen for indirect ELISA in order to monitor the immune response in vaccinated farm animals.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"57-61"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of cell-free supernatants of Pedicoccous acidilactici and Latilactobacillus sakei/Staphylococcus xylosus in combination with ethylenediaminetetraacetic acid against Escherichia coli O157:H7 strains. 乙酰二胺四乙酸联合乙酸对大肠埃希菌O157:H7的抑制作用

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2025.2035776.4368

Gökhan Kürşad İncili, Osman İrfan İlhak, Mehmet Çalıcıoğlu

{"title":"Inhibitory effect of cell-free supernatants of Pedicoccous acidilactici and Latilactobacillus sakei/Staphylococcus xylosus in combination with ethylenediaminetetraacetic acid against Escherichia coli O157:H7 strains.","authors":"Gökhan Kürşad İncili, Osman İrfan İlhak, Mehmet Çalıcıoğlu","doi":"10.30466/vrf.2025.2035776.4368","DOIUrl":"10.30466/vrf.2025.2035776.4368","url":null,"abstract":"The current research was carried out to evaluate in vitro anti-microbial properties of the cell-free supernatants (CFSs) derived from Pediococcus acidilactici (PA) and Latilactobacillus sakei/ Staphylococcus xylosus (LS) against Escherichia coli O157:H7 American Type Culture Collection strains (35150, 43894, and 43985). For this purpose, the diameters of zone of inhibition of the CFSs against E. coli O157:H7 strains were measured. In addition, a time-kill assay was conducted to determine the inhibitory effect of the CFSs alone or in combination with ethylenediaminetetraacetic acid (EDTA) during incubation at 37.00 ˚C for 24 hr. In the time-kill assay, E. coli O157:H7 was subjected to three concentrations of CFSs (1.00, 5.00, and 10.00%) and EDTA (0.02 M) in tryptic soy broth and the E. coli O157:H7 count was determined at 0, 6, and 24 hr intervals. The CFS of LS had a lower pH and higher titratable acidity compared to the PA. The CFS of LS displayed higher zones of inhibition than the CFS of PA against E. coli O157:H7 American Type Culture Collection 35150 and 43894 strains. The concentrations of 5.00 and 10.00% CFSs in combination with EDTA provided a 5.00 log10 decline in E. coli O157:H7 count over a 24-hr period. The results of this study indicated that the combination of CFSs (5.00 and 10.00%) and EDTA (0.02 M) exhibited an enhanced anti-bacterial effect against E. coli O157:H7 strains, which are substantial foodborne pathogenic bacteria.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"439-446"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of apilarnil against intense exercise-induced liver injury in rats: serological and histopathological evidence. 阿匹尼尔对大鼠剧烈运动所致肝损伤的保护作用：血清学和组织病理学证据。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2025.2048266.4580

Fatih Çakar, Halil Şimşek, Enes Kaya, Aykut Ulucan, Aydın Şükrü Bengü, Aydın Sever

{"title":"Protective role of apilarnil against intense exercise-induced liver injury in rats: serological and histopathological evidence.","authors":"Fatih Çakar, Halil Şimşek, Enes Kaya, Aykut Ulucan, Aydın Şükrü Bengü, Aydın Sever","doi":"10.30466/vrf.2025.2048266.4580","DOIUrl":"10.30466/vrf.2025.2048266.4580","url":null,"abstract":"Intense exercise is known to induce oxidative stress and inflammation, potentially leading to liver damage. This study examined the potential protective effects of apilarnil (AP), a natural bioactive compound with antioxidant and anti-inflammatory properties, against exercise-induced liver injury. Thirty-five male Wistar albino rats were allocated into five groups: Control, non-exercise (NEX), exercise (EX), EX + AP1 (0.20 g kg-1) and EX + AP2 (0.40 g kg-1). At the end of the 14-day experiment, serum and liver tissue samples were collected for the analysis of histopathological changes, oxidative stress markers, inflammatory cytokines and serum biochemical parameters. Histopathological evaluations revealed substantial liver damage in the EX group. However, in the EX + AP1 and EX + AP2 groups, the severity of these lesions was significantly attenuated. Biochemical analyses demonstrated elevated levels of tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 in the NEX group were markedly reduced by AP supplementation. Similarly, malondialdehyde levels were increased, while the activities of antioxidant enzymes - catalase, superoxide dismutase and glutathione peroxidase were declined in the NEX group. AP supplementation reversed these effects by lowering malondialdehyde levels and enhancing antioxidant enzyme activities in the EX + AP1 and EX + AP2 groups. Additionally, serum biochemical analyses indicated improved lipid profiles and liver function parameters in the AP -treated groups compared to the NEX group. In conclusion, histopathological and biochemical findings indicated that AP supplementation mitigated exercise-induced liver damage by reducing oxidative stress and inflammation, while enhancing antioxidant defenses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"421-430"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0