Veterinary Research Forum最新文献

First case of serpentovirus infection in a ball python (Python regius) in Thailand: a case report with molecular characterization. 泰国第一例蛇状病毒感染球蟒（蟒蛇）：一例分子特征的病例报告。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2034234.4345

Panithi Laoungbua, Nararat Laopichienpong, Apinya Longya, Lawan Chanhome, Taksa Vasaruchapong, Tanapong Tawan, Narongsak Chaiyabutr

{"title":"First case of serpentovirus infection in a ball python (Python regius) in Thailand: a case report with molecular characterization.","authors":"Panithi Laoungbua, Nararat Laopichienpong, Apinya Longya, Lawan Chanhome, Taksa Vasaruchapong, Tanapong Tawan, Narongsak Chaiyabutr","doi":"10.30466/vrf.2024.2034234.4345","DOIUrl":"10.30466/vrf.2024.2034234.4345","url":null,"abstract":"The pet snake industry in Thailand has seen a significant rise in popularity, with the ball python (Python regius) becoming a frequently kept species. However, respiratory disease poses a notable health concern, and various viral pathogens, including serpentoviruses (formerly classified as nidoviruses), have been implicated. While serpentovirus infections have been reported globally in diverse snake species, no documented cases had previously been identified in Thailand. This case report describes a 9-month-old ball python presenting to the Reptile Science Clinic at the Queen Saovabha Memorial Institute in Bangkok, Thailand, with respiratory distress and emaciation. Despite veterinary intervention, the snake succumbed to the infection within two weeks. Post-mortem examination revealed marked mucus accumulation within the oral cavity and necrotic oral mucosa. Histopathological analysis demonstrated severe catarrhal pneumonia. Molecular investigations confirmed the presence of serpentovirus in the lung tissue of the affected python, with subsequent sequence analysis revealing close homology to known serpentoviruses in ball pythons. This report documents the first confirmed case of serpentovirus infection in a pet snake in Thailand.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"361-364"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical evidence of lipid peroxidation role in diethylnitrosamine-induced hepatocellular carcinoma in male Wistar albino rats. 脂质过氧化作用在雄性Wistar白化大鼠二乙基亚硝胺诱导的肝细胞癌中的免疫组织化学证据。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2036652.4378

Emin Karakurt, Serpil Dag, Hilmi Nuhoglu, Ayfer Yıldız, Emre Kurtbas

{"title":"Immunohistochemical evidence of lipid peroxidation role in diethylnitrosamine-induced hepatocellular carcinoma in male Wistar albino rats.","authors":"Emin Karakurt, Serpil Dag, Hilmi Nuhoglu, Ayfer Yıldız, Emre Kurtbas","doi":"10.30466/vrf.2024.2036652.4378","DOIUrl":"10.30466/vrf.2024.2036652.4378","url":null,"abstract":"This study aimed to evaluate malondialdehyde (MDA) expressions using the immunohisto-chemical method in order to reveal the role of lipid peroxidation in the development and progression of hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN) in male Wistar albino rats. Avidin-biotin-peroxidase method was used for immunohistochemical evaluation. Histopathological examinations revealed that DEN caused a mixed pattern (trabecular and acinar formations) of HCC in the majority of rats. The MDA positive stainings were significantly increased in rats in the HCC group compared to the healthy rats in the control group. In conclusion, this study data contain three important findings. The first one is that DEN triggers the formation of reactive oxygen species (ROS), excessively produced ROS cause oxidative stress, and as a result, oxidative stress strongly causes lipid peroxidation. Secondly, it is clear that there is an important relationship between oxidative stress-induced lipid peroxidation and HCC progression. At the same time, MDA is an useful biomarker in determining the prognosis of patients with HCC. The third and final finding is that intra-peritoneal DEN injection once a week for 20 weeks, but not in combination with other promoting chemical agents, appears to be very effective in inducing experimental HCC.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"375-380"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological changes in fibrous tissue of rat myocardium after administration of dispersed allogeneic biomaterial. 分散异体生物材料对大鼠心肌纤维组织形态学的影响。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-07-15 DOI: 10.30466/vrf.2024.2035761.4367

Lebedeva Anna Ivanovna, Musina Lyalya Akhiyarovna, Prusakov Alexey Viktorovich, Vladimir Sergeevich Ponamarev

{"title":"Morphological changes in fibrous tissue of rat myocardium after administration of dispersed allogeneic biomaterial.","authors":"Lebedeva Anna Ivanovna, Musina Lyalya Akhiyarovna, Prusakov Alexey Viktorovich, Vladimir Sergeevich Ponamarev","doi":"10.30466/vrf.2024.2035761.4367","DOIUrl":"10.30466/vrf.2024.2035761.4367","url":null,"abstract":"To improve the structure of the heart muscle after myocardial infarction, methods of regenerative medicine are used. One of the promising areas is the intra-myocardial administration of acellular allogeneic biomaterial (AB). The AB stimulates the regeneration of organs and tissues. But, the effect of AB on the myocardium after its fibrous ischemic degeneration has not been assessed. The aim of the study was to assess the morphological structure of the heart after cryodestruction in the late period and the use of AB. Chronic myocardial infarction was modeled in 80 male rats. To simulate chronic myocardial infarction and fibrosis formation, contact cryodestruction was performed. After 45 days, during repeated thoracotomy in the main group, AB suspension was injected into the area of the cryogenic myocardial scar. Six injections of 0.50 mg of dry substance were administered. In the control group, physiological solution was injected. After AB administration, following 7, 14, 30, and 45 days, the animals were withdrawn from the experiment, and the hearts were excised for histological and immunohistochemical studies. The AB underwent gradual phagocytosis by macrophages and gradually replaced by loose fibrous connective tissue with the presence of cardiac troponin I+ labeled muscle cells, which over time underwent hypertrophy. Cardiomyocytes were grouped in the AB implantation zone as separate clusters. The heart mass did not change in both experimental groups. The use of AB in the area of the formed cryogenic myocardial scar promoted the transformation of dense fibrous connective tissue into loose tissue and its replacement with cardiac muscle tissue.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 7","pages":"381-386"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry. 伊朗卡尚地区肉鸡4型禽腺病毒的分子和组织病理学特征：对家禽业疾病控制和预防的意义

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2030590.4302

Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi

{"title":"Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry.","authors":"Mohammadreza Ghorani, Amir Ali Shahbazfar, Mohsen Ghorbiani, Behzad Ghorbanzadeh, Rohollah Kamyabi","doi":"10.30466/vrf.2024.2030590.4302","DOIUrl":"https://doi.org/10.30466/vrf.2024.2030590.4302","url":null,"abstract":"Fowl adenovirus (FAdV) is a DNA virus causing significant diseases, like inclusion body hepatitis, hydropericardium-hepatitis syndrome (HHS), and gizzard erosion. These diseases lead to severe economic losses in the poultry industry. Recent increases in HHS outbreaks in Iran, particularly among broilers, prompted this study to analyze FAdV isolates in Kashan, Iran. In December 2021, a high-mortality HHS outbreak in a Kashan broiler flock led to liver and heart samples being sent for analysis. Histopathological investigations revealed mononuclear hepatitis and intra-nuclear viral inclusion bodies in hepatocytes. Polymerase chain reaction and phylogenetic analyses confirmed the presence of FAdV-4 (accession number: PP856395), showing 99.99% identity with strains from Japan, the United Arab Emirates, Pakistan, and the United States. These findings highlight the genetic similarity and potential common origin of FAdV-4 strains. This study emphasizes the need for heightened biosecurity measures and effective vaccination strategies to mitigate the spread of FAdV-4. The confirmed presence of FAdV-4 in central Iran poses a significant threat to the poultry industry, necessitating prompt action to prevent substantial economic losses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"19-25"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine. 黑腿病疫苗抗体检测用邵氏梭状芽胞杆菌细胞表面蛋白的纯化方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI: 10.30466/vrf.2024.2023812.4190

Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri

{"title":"A simple cost-effective method for purification of Clostridium chauvoei cell-surface proteins for detection of antibodies against blackleg disease vaccine.","authors":"Niusha Adib, Azadeh Zahmatkesh, Masoumeh Bagheri","doi":"10.30466/vrf.2024.2023812.4190","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023812.4190","url":null,"abstract":"Cell-surface proteins of Clostridium chauvoei were purified using a simple method. Bacterial cultures were centrifuged and agitated vigorously in phosphate buffered saline with or without further glycine treatment and ammonium sulfate precipitation. Rabbits were immunized subcutaneously with a blackleg disease vaccine twice with a two-week interval. Immunized sera were collected one week after the second injection. Enzyme-linked immunosorbent assay (ELISA) was performed using the proteins purified by the second method as the coating antigen. Bradford assay results showed a higher protein concentration in the second than the first method. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis analysis showed multiple bands for the cell-surface proteins of C. chauvoei in the first method and a sharp band equivalent to flagellin protein in the second method. The ELISA results indicated that the purified proteins were capable of detecting antibodies against Blackleg disease vaccine. The purified protein would be an alternative antigen for indirect ELISA in order to monitor the immune response in vaccinated farm animals.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 1","pages":"57-61"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of the Escherichia coli heme-utilization gene A virulence factor in E. coli isolated from the feces of horses in Sumbawa island, Indonesia. 印度尼西亚松巴哇岛马粪大肠杆菌中血红素利用基因A毒力因子的分子检测。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-06-15 DOI: 10.30466/vrf.2024.2039823.4421

Kholik Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Ieke Wulan Ayu, Ika Nurani Dewi

{"title":"Molecular detection of the Escherichia coli heme-utilization gene A virulence factor in E. coli isolated from the feces of horses in Sumbawa island, Indonesia.","authors":"Kholik Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Ieke Wulan Ayu, Ika Nurani Dewi","doi":"10.30466/vrf.2024.2039823.4421","DOIUrl":"10.30466/vrf.2024.2039823.4421","url":null,"abstract":"The transmission of Escherichia coli (E. coli) containing virulent genes from animals to humans and the environment poses significant public health challenges. This study aimed to detect the virulence factor of the E. coli heme-utilization gene A (chuA) in E. coli isolated from the feces of apparently healthy horses in the island of Sumbawa, Indonesia. The study utilized 52 fecal samples from a total horse population of 283, calculated using the disease detection formula. Fresh feces were collected immediately after excretion and placed in buffered peptone water for subsequent analysis. The samples were then isolated on eosin methylene blue media and identified using biochemical tests. Identified E. coli strains were further examined for detecting the chuA gene using polymerase chain reaction techniques. The E. coli was successfully isolated and identified in 11 (21.15%) of the 52 collected fecal samples. Polymerase chain reaction analysis detected the chuA gene in 8 (15.38%) E. coli isolates at 279 bp on gel electrophoresis. The close interaction between horses and humans in the island of Sumbawa, Indonesia, may facilitate the spread of E. coli. Thus, surveillance is needed to employ a One Health approach to monitor E. coli strains encoding the chuA gene and other virulence factors to control their dissemination.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"325-330"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of cell-free supernatants of Pedicoccous acidilactici and Latilactobacillus sakei/Staphylococcus xylosus in combination with ethylenediaminetetraacetic acid against Escherichia coli O157:H7 strains. 乙酰二胺四乙酸联合乙酸对大肠埃希菌O157:H7的抑制作用

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2025.2035776.4368

Gökhan Kürşad İncili, Osman İrfan İlhak, Mehmet Çalıcıoğlu

{"title":"Inhibitory effect of cell-free supernatants of Pedicoccous acidilactici and Latilactobacillus sakei/Staphylococcus xylosus in combination with ethylenediaminetetraacetic acid against Escherichia coli O157:H7 strains.","authors":"Gökhan Kürşad İncili, Osman İrfan İlhak, Mehmet Çalıcıoğlu","doi":"10.30466/vrf.2025.2035776.4368","DOIUrl":"10.30466/vrf.2025.2035776.4368","url":null,"abstract":"The current research was carried out to evaluate in vitro anti-microbial properties of the cell-free supernatants (CFSs) derived from Pediococcus acidilactici (PA) and Latilactobacillus sakei/ Staphylococcus xylosus (LS) against Escherichia coli O157:H7 American Type Culture Collection strains (35150, 43894, and 43985). For this purpose, the diameters of zone of inhibition of the CFSs against E. coli O157:H7 strains were measured. In addition, a time-kill assay was conducted to determine the inhibitory effect of the CFSs alone or in combination with ethylenediaminetetraacetic acid (EDTA) during incubation at 37.00 ˚C for 24 hr. In the time-kill assay, E. coli O157:H7 was subjected to three concentrations of CFSs (1.00, 5.00, and 10.00%) and EDTA (0.02 M) in tryptic soy broth and the E. coli O157:H7 count was determined at 0, 6, and 24 hr intervals. The CFS of LS had a lower pH and higher titratable acidity compared to the PA. The CFS of LS displayed higher zones of inhibition than the CFS of PA against E. coli O157:H7 American Type Culture Collection 35150 and 43894 strains. The concentrations of 5.00 and 10.00% CFSs in combination with EDTA provided a 5.00 log10 decline in E. coli O157:H7 count over a 24-hr period. The results of this study indicated that the combination of CFSs (5.00 and 10.00%) and EDTA (0.02 M) exhibited an enhanced anti-bacterial effect against E. coli O157:H7 strains, which are substantial foodborne pathogenic bacteria.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"439-446"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of apilarnil against intense exercise-induced liver injury in rats: serological and histopathological evidence. 阿匹尼尔对大鼠剧烈运动所致肝损伤的保护作用：血清学和组织病理学证据。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2025.2048266.4580

Fatih Çakar, Halil Şimşek, Enes Kaya, Aykut Ulucan, Aydın Şükrü Bengü, Aydın Sever

{"title":"Protective role of apilarnil against intense exercise-induced liver injury in rats: serological and histopathological evidence.","authors":"Fatih Çakar, Halil Şimşek, Enes Kaya, Aykut Ulucan, Aydın Şükrü Bengü, Aydın Sever","doi":"10.30466/vrf.2025.2048266.4580","DOIUrl":"10.30466/vrf.2025.2048266.4580","url":null,"abstract":"Intense exercise is known to induce oxidative stress and inflammation, potentially leading to liver damage. This study examined the potential protective effects of apilarnil (AP), a natural bioactive compound with antioxidant and anti-inflammatory properties, against exercise-induced liver injury. Thirty-five male Wistar albino rats were allocated into five groups: Control, non-exercise (NEX), exercise (EX), EX + AP1 (0.20 g kg-1) and EX + AP2 (0.40 g kg-1). At the end of the 14-day experiment, serum and liver tissue samples were collected for the analysis of histopathological changes, oxidative stress markers, inflammatory cytokines and serum biochemical parameters. Histopathological evaluations revealed substantial liver damage in the EX group. However, in the EX + AP1 and EX + AP2 groups, the severity of these lesions was significantly attenuated. Biochemical analyses demonstrated elevated levels of tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 in the NEX group were markedly reduced by AP supplementation. Similarly, malondialdehyde levels were increased, while the activities of antioxidant enzymes - catalase, superoxide dismutase and glutathione peroxidase were declined in the NEX group. AP supplementation reversed these effects by lowering malondialdehyde levels and enhancing antioxidant enzyme activities in the EX + AP1 and EX + AP2 groups. Additionally, serum biochemical analyses indicated improved lipid profiles and liver function parameters in the AP -treated groups compared to the NEX group. In conclusion, histopathological and biochemical findings indicated that AP supplementation mitigated exercise-induced liver damage by reducing oxidative stress and inflammation, while enhancing antioxidant defenses.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"421-430"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible mechanism of protective effect of melatonin against carbendazim-induced hepatotoxicity in mature male rats: histological, immunofluorescence, and biochemical evaluations. 褪黑素对多菌灵诱导的成熟雄性大鼠肝毒性保护作用的可能机制：组织学、免疫荧光和生化评价。

IF 1.1 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.30466/vrf.2024.2041021.4455

Ali Menatnia, Ali Louei Monfared, Hassaneen Sharoot

{"title":"Possible mechanism of protective effect of melatonin against carbendazim-induced hepatotoxicity in mature male rats: histological, immunofluorescence, and biochemical evaluations.","authors":"Ali Menatnia, Ali Louei Monfared, Hassaneen Sharoot","doi":"10.30466/vrf.2024.2041021.4455","DOIUrl":"10.30466/vrf.2024.2041021.4455","url":null,"abstract":"This study investigated carbendazim (CBZ)-induced hepatic dysfunction and the mechanistic pathway regarding the protective effect of melatonin (MEL). Twenty-eight male rats were grouped as follows: Control, CBZ (150 mg kg-1), MEL (20.00 mg kg-1), and CBZ + MEL. The experiment was conducted for 60 days. Tissue samples were stained with Hematoxylin and Eosin and immuno-fluorescence methods to examine apoptotic pathway. Also, hepatic enzymes and miR-122 expression were evaluated. The findings indicated that the CBZ group exhibited an increase in degenerated hepatocytes, hyperemia of sinusoids, and leukocyte infiltration, accompanied by elevated levels of aspartate aminotransferase and alanine aminotransferase, as well as up-regulation of miR-122. Also, there was a significant increase in the fluorescence intensities of caspase-3 and Bax in the CBZ group, whereas a substantial reduction in the fluorescence intensity of Bcl-2 was recorded. In contrast, the simultaneous administration of MEL alongside CBZ was shown to be effective in improving histological structure, decreasing levels of aspartate aminotransferase and alanine aminotransferase, reducing the apoptosis index, and modulating the expression of miR-122 in comparison with the CBZ-only group. The increased expression of miR-122 noted in the CBZ group may correlate with an elevation in the immunoreactivity of apoptosis markers and alterations in liver architecture. Additionally, MEL seems to alleviate CBZ-induced hepatotoxicity by down-regulating miR-122 expression, diminishing the fluorescence intensity of caspase-3 and Bax, and enhancing the immunoreactivity of Bcl-2. Collectively, the regulation of miR-122 may serve as a potential mechanism by which MEL confers its protective effects against liver damage induced by CBZ.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"465-473"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats. 百里醌通过抗氧化和抗炎机制减轻大鼠爪切引起的自发性和诱发性疼痛。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2025-01-01 Epub Date: 2025-02-15 DOI: 10.30466/vrf.2024.2025053.4204

Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh

{"title":"Thymoquinone attenuates paw incision-induced spontaneous and evoked pain through anti-oxidative and anti-inflammatory mechanisms in rats.","authors":"Seyed Siavash Ghoreishi, Saeed Azizi, Esmaeal Tamaddonfard, Safiyeh Aghazadeh","doi":"10.30466/vrf.2024.2025053.4204","DOIUrl":"10.30466/vrf.2024.2025053.4204","url":null,"abstract":"Surgery commonly causes post-operative pain that should be alleviated to prevent complications. In addition to the use of synthetic drugs, there has been a widespread desire to use medicinal plants for surgical pain management. Thymoquinone (TQ), a constituent of Nigella sativa black seeds, exhibits a potent anti-oxidant property. Celecoxib (CLX), a potent non-steroidal anti-inflammatory drug, is widely used in pain management. In the present study, the effects of TQ and CLX on pain caused by hind paw surgical incision were compared. Fifty-six rats were divided into four groups of 14 rats as intact, vehicle, TQ, and CLX groups. In each group, six rats were planned to record pain-related behaviors on days 1 - 10 and eight rats were designed for determination of serum biochemical alterations on days 1 (four rats) and 3 (four rats) after surgery. Oral administrations of TQ and CLX at a same dose of 10.00 mg kg-1 alleviated paw lifting number (spontaneous pain) and paw withdrawal threshold evoked by von Frey filaments on metal mesh floor, improved the decreased contents of serum total anti-oxidant capacity and superoxide dismutase, and restored the increased levels of serum malondialdehyde and tumor necrosis factor-alpha. The results suggested that TQ by employing anti-oxidant and anti-inflammatory mechanisms, might relieve the pain induced by hind paw plantar incision, being comparable with CLX.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"89-96"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0