{"title":"从牛乳中分离的肺炎克雷伯氏菌产生毒力和广谱β-内酰胺酶基因的遗传特征。","authors":"Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki","doi":"10.30466/vrf.2023.2006974.3947","DOIUrl":null,"url":null,"abstract":"<p><p>Mastitis associated <i>Klebsiella pneumoniae</i> species were isolated from bovine milk to characterize virulence genes (<i>wabG</i> and <i>kfuBC</i>) and extended spectrum β-lactamase (ESBL) genes (<i>bla</i><sub>CTX-M-1</sub>, <i>bla</i><sub>CTX-M-2</sub>, <i>bla</i><sub>CTX-M-9</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>SHV</sub> and <i>bla</i><sub>OXA</sub>). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 <i>K. pneumoniae</i> isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of <i>wabG</i> (25/27; 92.59%) was higher than <i>kfuBC</i> (5/27; 18.51%). Beta-lactamase genes <i>viz., bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>CTX-M-1</sub> were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for <i>bla</i><sub>CTX-M-9</sub> and <i>bla</i><sub>OXA-1</sub> genes. Outcome of the study provided an insight into virulence genes and ESBL producing <i>K. pneumoniae</i> isolated from bovine milk samples in India.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924294/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genetic characterization of virulence and extended spectrum β-lactamase producing genes of <i>Klebsiella pneumoniae</i> isolated from bovine milk.\",\"authors\":\"Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki\",\"doi\":\"10.30466/vrf.2023.2006974.3947\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mastitis associated <i>Klebsiella pneumoniae</i> species were isolated from bovine milk to characterize virulence genes (<i>wabG</i> and <i>kfuBC</i>) and extended spectrum β-lactamase (ESBL) genes (<i>bla</i><sub>CTX-M-1</sub>, <i>bla</i><sub>CTX-M-2</sub>, <i>bla</i><sub>CTX-M-9</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>SHV</sub> and <i>bla</i><sub>OXA</sub>). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 <i>K. pneumoniae</i> isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of <i>wabG</i> (25/27; 92.59%) was higher than <i>kfuBC</i> (5/27; 18.51%). Beta-lactamase genes <i>viz., bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>CTX-M-1</sub> were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for <i>bla</i><sub>CTX-M-9</sub> and <i>bla</i><sub>OXA-1</sub> genes. Outcome of the study provided an insight into virulence genes and ESBL producing <i>K. pneumoniae</i> isolated from bovine milk samples in India.</p>\",\"PeriodicalId\":23989,\"journal\":{\"name\":\"Veterinary Research Forum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924294/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Research Forum\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.30466/vrf.2023.2006974.3947\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Forum","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.30466/vrf.2023.2006974.3947","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
Genetic characterization of virulence and extended spectrum β-lactamase producing genes of Klebsiella pneumoniae isolated from bovine milk.
Mastitis associated Klebsiella pneumoniae species were isolated from bovine milk to characterize virulence genes (wabG and kfuBC) and extended spectrum β-lactamase (ESBL) genes (blaCTX-M-1, blaCTX-M-2, blaCTX-M-9, blaTEM, blaSHV and blaOXA). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 K. pneumoniae isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of wabG (25/27; 92.59%) was higher than kfuBC (5/27; 18.51%). Beta-lactamase genes viz., blaSHV, blaTEM and blaCTX-M-1 were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for blaCTX-M-9 and blaOXA-1 genes. Outcome of the study provided an insight into virulence genes and ESBL producing K. pneumoniae isolated from bovine milk samples in India.
期刊介绍：
Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.
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