Genetic characterization of virulence and extended spectrum β-lactamase producing genes of Klebsiella pneumoniae isolated from bovine milk.

IF 0.8 4区农林科学 Q3 ZOOLOGY

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI:10.30466/vrf.2023.2006974.3947

Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki

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Abstract

Mastitis associated Klebsiella pneumoniae species were isolated from bovine milk to characterize virulence genes (wabG and kfuBC) and extended spectrum β-lactamase (ESBL) genes (bla_CTX-M-1, bla_CTX-M-2, bla_CTX-M-9, bla_TEM, bla_SHV and bla_OXA). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 K. pneumoniae isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of wabG (25/27; 92.59%) was higher than kfuBC (5/27; 18.51%). Beta-lactamase genes viz., bla_SHV, bla_TEM and bla_CTX-M-1 were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for bla_CTX-M-9 and bla_OXA-1 genes. Outcome of the study provided an insight into virulence genes and ESBL producing K. pneumoniae isolated from bovine milk samples in India.

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从牛乳中分离的肺炎克雷伯氏菌产生毒力和广谱β-内酰胺酶基因的遗传特征。

研究人员从牛乳中分离出与乳腺炎相关的肺炎克雷伯氏菌，以鉴定其毒力基因（wabG 和 kfuBC）和广谱β-内酰胺酶（ESBL）基因（blaCTX-M-1、blaCTX-M-2、blaCTX-M-9、blaTEM、blaSHV 和 blaOXA）。研究共收集了 325 份牛乳样本（195 份生乳样本和 130 份乳汁样本），这些样本来自印度古吉拉特邦的巴纳斯坎塔（Banaskantha）牛奶区。共分离出 27 株肺炎克氏菌，其中 17 株（62.96%）从生牛乳中分离出来，10 株（37.03%）从牛奶样本中分离出来，总感染率为 8.31%。抗生素敏感性模式显示，27 个分离株中有 20 个具有多重耐药性。根据组合盘扩散试验和 HiCrome ESBL 琼脂法，分别有 20 株（74.07%）和 25 株（92.59%）分离菌株被检测出产 ESBL。在所研究的毒力基因中，wabG（25/27；92.59%）高于 kfuBC（5/27；18.51%）。23/27（85.18%）、3/27（11.11%）和 2/27（7.40%）的分离物中分别检测到β-内酰胺酶基因，即 blaSHV、blaTEM 和 blaCTX-M-1；而没有发现任何分离物的 blaCTX-M-9 和 blaOXA-1 基因呈阳性。研究结果有助于深入了解从印度牛乳样本中分离出的肺炎克氏菌的毒力基因和产ESBL的情况。

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来源期刊

Veterinary Research Forum Veterinary-General Veterinary

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.