Veterinary Research Forum最新文献

{"title":"Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after spinal cord injury.","authors":"Shiva Amanollahi, Ahmad Reza Bahrami, Azadeh Haghighitalab, Hanieh Shaterzadeh Yazdi, Hossein Kazemi Mehrjerdi","doi":"10.30466/vrf.2023.2003942.3903","DOIUrl":"10.30466/vrf.2023.2003942.3903","url":null,"abstract":"<p><p>Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human <i>indoleamine 2,3-dioxygenase-1</i> (<i>IDO1</i>) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of 72-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and <i>IDO1</i>-EVs. The 60.00 µL of hydrogel or hydrogels containing 100 µg sEVs from GFP or <i>IDO1</i>-EVs were locally injected immediately after SCI (laminectomy of the T10 vertebra and clip compression). After 8 weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received <i>IDO1</i>-EVs (3.52 ± 0.04) was increased in comparison with the SCI group (3.00 ± 0.94). Also, these animals indicated higher glucose levels in their serum (250.17 ± 69.61) in comparison with SCI ones (214 ± 45.34). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of <i>IDO1</i>-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"27-34"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the effects of different DHA/EPA ratios in rainbow trout (<i>Oncorhynchus mykiss</i>) egg composition on foregut development of larvae.","authors":"Dana Dadras, Ahmad Imani, Naser Agh, Rasoul Shahrooz, Parisa Mohammadisefat","doi":"10.30466/vrf.2024.2013685.4035","DOIUrl":"10.30466/vrf.2024.2013685.4035","url":null,"abstract":"<p><p>Lecithotrophic larvae utilize extensive yolk reserves for early development. In this study, the effect of egg docosahexaenoic acid (DHA):eicosapentaenoic acid (EPA) ratios (<i>i.e.</i>, 5.92, 10.08, 11.66, and 14.53) on the emerging larvae foregut development of rainbow trout was examined. Larvae samples were taken from day 22 to 36 post-fertilization. Thin whole body longitudinal sections were prepared and stained by Hematoxylin and Eosin and Alcian blue procedure. The sections were examined regarding epithelial layer thickness, intestinal fold height and mucosal layer thickness along with number of enterocytes and goblet cells. Results indicated that maximum thickness of the epithelium was observed on day 36 post fertilization in larvae hatched from eggs with DHA:EPA ratios of 14.53 and 10.08. The highest and lowest intestinal folds height were also observed in larvae hatched from eggs with DHA:EPA ratios of 10.08 and 14.53, respectively. The mucosal-submucosa layer thickness was the highest in larvae hatched from eggs with DHA:EPA ratio of 10.08. Enterocyte's count was the highest in larvae obtained from eggs with DHA:EPA ratio of 10.08 on day 36 post-fertilization. The highest and lowest number of goblet cells were enumerated in larvae obtained from eggs with DHA:EPA ratios of 5.53 and 14.53, respectively. In conclusion, our results revealed that feeding rainbow trout broodstock with diet contained highly unsaturated fatty acids (HUFA):polyunsaturated fatty acids (PUFA) ratio of 0.28 could result in the egg with DHA:EPA ratio of 10.08 which in turn yielded larvae with better foregut development parameters compared to those larvae emerged from the eggs with increased DHA :EPA ratio.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"463-471"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of glucose-insulin homeostasis at different stages of gestation in cows.","authors":"Alekhin Yuri Nikolaevich, Popova Olga Sergeevna, Ponamarev Vladimir Sergeevich","doi":"10.30466/vrf.2024.2023763.4188","DOIUrl":"10.30466/vrf.2024.2023763.4188","url":null,"abstract":"<p><p>An increase in morbidity during times of relatively high functional load on the body such as during pregnancy, confirms the role of metabolic overload in the occurrence of metabolic failures. It is better to take preventive measures such as adjusting metabolic regulation mechanisms in light of the ideal dietary composition. However, this direction is constrained by the lack of information about neurohumoral regulation. The goal of the present study was to learn more about the dynamics of changes in insulin and glucose levels in pregnant cow's blood. Research on the levels of glucose and insulin in lactating cows demonstrated that ruminants had distinct characteristics in the metabolism of carbohydrates, most notably a lessened reliance of blood glucose on insulin levels. A rise in blood glucose and insulin levels was observed as the gestational stage increased during the third trimester of pregnancy. The intensity of this insulin tolerance was contingent upon the level of productivity and glucose levels during the preceding stages of gestation.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"523-528"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological evaluation of the viability of auto-transplanted ovary following vitrification-thawing process in rabbit as an animal model.","authors":"Ensiye Sajadian Jaghargh, Mohammad Taghi Vajed Ebrahimi, Armin Attaranzadeh, Abbas Parham, Ahmad Reza Mohammadnia","doi":"10.30466/vrf.2024.2025342.4218","DOIUrl":"10.30466/vrf.2024.2025342.4218","url":null,"abstract":"<p><p>Cryopreservation and re-transplantation of ovarian tissue is a relatively new technique to preserve fertility. This study evaluated the preservation of ovarian follicles after the vitrification-thawing procedure by implanting ovarian fragments into the gluteus muscle of rabbit. Bilateral ovariectomy was performed on each rabbit. The cortices were separated from the medullae of ovaries and divided into four fragments. One fresh cortical fragment was used as a control, fixed in 10.00% formalin. Three fragments underwent vitrification-thawing for two weeks. Two of these vitrified-thawed fragments were auto-transplanted into separate locations within the opened gluteus muscle, while the final fragment was fixed in 10.00% formalin. Eight weeks after re-implantation, biopsies were collected from the ovarian fragments and fixed in 10.00% formalin. The numbers and areas (µm²) of morphologically normal follicles were evaluated on sections stained with Hematoxylin and Eosin through light microscopy. The follicular morphology in the ovarian fragments immediately after vitrification-thawing remained similar to that of the fresh ovary. The number of morphologically intact follicles was significantly lower in the ovarian fragments eight weeks after re-implantation than fresh ovary and ovarian tissue fragments after vitrification-thawing procedure. However, follicular development to the antral stage was observed in all samples eight weeks after re-implantation. There was no statistical difference in the areas (µm²) of primordial, primary, pre-antral, and antral follicles in the ovarian fragments before cryopreservation, immediately after vitrification-thawing, and following re-implantation procedures. Auto-transplantation of ovarian tissue into the gluteus muscle of rabbits could be a viable approach for preserving ovarian follicles after vitrification-thawing procedure.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"545-550"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological characterization of recombinant outer membrane Loa22 protein of local pathogenic <i>Leptospira</i> serovars.","authors":"Mehdi Gharakhani, Mohammad Faezi Ghasemi, Pejvak Khaki, Majid Esmaelizad, Majid Tebianian","doi":"10.30466/vrf.2024.2017700.4099","DOIUrl":"10.30466/vrf.2024.2017700.4099","url":null,"abstract":"<p><p>Leptospirosis is a worldwide zoonotic disease caused by pathogenic <i>Leptospira</i> spp<i>.</i>, often occurring in tropical and subtropical regions. Focusing on development of rapid diagnostic methods to facilitate early diagnosis and a universal vaccine are the main critical issues to overcome the burden of leptospirosis. Here, we have studied the immunogenic potential of prepared recombinant Loa22 protein (rLoa22) of local pathogenic <i>Leptospira</i> species in mice and its ability to induce humoral and cellular immunity, being further evaluated by analyzing the immunoglobulin G (IgG) subclasses and cytokines produced through immunization. Based on the results, mice immunized with rLoa22/adjuvant and a trivalent vaccine, induced high titers of total IgG. All immunized groups increased IgG1 almost on the same level; but, IgG2a level was significantly higher in the vaccine and rLoa22/adjuvant groups than rLoa22 alone group. Animals immunized with the vaccine produced more interleukin 4 than rLoa22/ adjuvant group. The results of evaluating interferon gamma level showed that the rLoa22/adjuvant and vaccine groups had a significant increase compared to the rLoa22 alone group. The results also demonstrated that the rLoa22 protein, in indirect enzyme-linked immunosorbent assay, was able to detect the anti-<i>Leptospira</i> antibodies in mice serum that can be used as a marker in assessing the seroprevalence of leptospirosis and/or in combination with other leptospiral antigens in development of an effective vaccine against leptospirosis.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"565-573"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of biogenic amine production of different lactic acid bacteria isolated from ready-to-eat packaged fish products.","authors":"Nurten Yilmaz","doi":"10.30466/vrf.2024.2024103.4193","DOIUrl":"10.30466/vrf.2024.2024103.4193","url":null,"abstract":"<p><p>The ability of lactic acid bacteria (LAB) to produce biogenic amines (BAs) from amino acids using amino acid decarboxylase enzymes is an important food safety criterion due to their use as starter or bio-protective cultures. In this study, various LAB isolates, including <i>Lactococcus</i> (three isolates), <i>Lactobacillus</i> (five isolates), <i>Enterococcus</i> (13 isolates), and <i>Leuconostoc</i> (seven isolates), were isolated from ready-to-eat fish products like sauced, marinated, smoked anchovies, and smoked mackerel. These isolates were then tested for their ability to produce BAs (histamine, putrescine, cadaverine, spermidine, tryptamine, 2-phenylethylamine, spermine, serotonin, tyramine, 3-methylamine, and dopamine) and ammonia in histidine decarboxylase broth. The post-biotic solutions (cell-free supernatant) of <i>Lb. paracasei</i> NZ.Lbp.111, <i>Lb. brevis</i> NZ.Lbb.085, and <i>E. casseliflavus</i> NZ.Ec.074 exhibited the highest ammonia levels. Histamine accumulation was generally low in post-biotic solutions from four LAB isolates, ranging from 2.00 to 7.78 ppm. However, the post-biotic solution of <i>Leu. mesenteroides</i> NZLeu 009 displayed a significantly higher histamine concentration (83.23 ppm). <i>Lactobacillus</i> post-biotic solutions had the highest concentrations of ammonia (1220.28 ppm), 2-phenylethylamine (82.96 ppm), serotonin (278.70 ppm), tyramine (267.48 ppm), and cadaverine (19.72 ppm). Also, the average BAs concentration for <i>Lactobacillu</i>s post-biotic solutions was the highest at 31.98 ppm. The results revealed the analysis of BAs concentrations in different LAB isolates from fish products, providing interesting insights into their metabolic capabilities and potential implications for food safety and quality.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"537-543"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a lytic bacteriophage against clinical isolates of <i>Salmonella typhimurium</i> in turkey poults.","authors":"Masoud Amini, Abdolghaffar Ownagh, Amir Tukmechi, Manoochehr Allymehr","doi":"10.30466/vrf.2023.2000885.3864","DOIUrl":"10.30466/vrf.2023.2000885.3864","url":null,"abstract":"<p><p>The poultry products are known as a source of zoonotic and multi-drug resistant pathogens, especially <i>Salmonella</i> spp. The objective of this study was using bacteriophages as an alternative anti-microbial agent against <i>Salmonella</i> <i>typhimurium</i> isolate from turkey poults. The antibiotic susceptibility test was used to identify the antibiotic resistance pattern of the isolates. The bacteriophage was purified, enhanced and titrated using the Spot test and double layer agar (DLA) techniques after being isolated from a chicken slaughterhouse and sewage treatment facility<i>.</i> By determining the morphological characteristics of resulting plaque, the specificity and host range of the phage were studied on <i>S. typhimurium</i> isolates. A total number of 22 suspected <i>Salmonella</i> isolates were confirmed biochemically positive in sample by cultures method. Nine of these isolates (40.90%) were identified as <i>S. typhimurium</i> by polymerase chain reaction. All of isolates (100%) were resistant to chloramphenicol, doxycycline, kanamycin, florfenicol, rifampin, and erythromycin. Seven isolates (77.77%) were resistant to amoxicillin and nalidixic acid. The plaques were present with 3.00 ± 0.22 mm in diameter on the culture of 6 out of 9 (66.66%) isolates of <i>S.</i> <i>typhimurium</i> on brain heart infusion broth using DLA method. The amount of phage titer was 7.60 × 10⁷ phage forming unit mL^-1 and its multiplicity of infection value was calculated as 5.06 × 10^-2 based on obtained results. In place of antibiotics, the multi-drug resistant (MDR) <i>S. typhimurium</i> was successfully destroyed by the isolated bacteriophage from wastewater. <i>In vitro</i> settings were used in this investigation to identify the efficient bacteriophages against MDR <i>S. typhimurium</i>.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"309-316"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence, characteristics, and antibiotic sensitivity of <i>Staphylococcus aureus</i> isolated from wild birds in the Kasur district of Punjab, Pakistan.","authors":"Tooba Latif, Shahzad Ali, Arshad Javid, Ali Ahmad Sheikh","doi":"10.30466/vrf.2024.2009978.3990","DOIUrl":"10.30466/vrf.2024.2009978.3990","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is gaining worldwide attention because of its substantial impact on public health. The current study aimed to characterize <i>S. aureus</i> strains isolated from wild birds in the Kasur district of Punjab, Pakistan from 2021 to 2022. A total of one hundred samples were collected from five wild bird species. The samples were enriched, inoculated on selective agars and cultured for 24 hr at 37.00 ˚C. All isolates were verified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) after Gram staining. Positive isolates were screened for phenotypic (Kirby-Bauer disk diffusion and minimum inhibitory concentration s), genotypic antibiotic resistance, and virulence genes. These samples yielded 30 (30.00%) <i>S. aureus</i> isolates, confirmed by polymerase chain reaction utilizing the 16S rRNA gene. <i>Staphylococcus aureus</i> was more prevalent in cloacal samples (16.00%) than oral samples (14.00%). Various <i>S. aureus</i> isolates showed varying degrees of resistance to three different antibiotics. Oxacillin (56.66%; n = 17) and tetracycline (33.33%; n = 10) showed the highest resistance rates with the lowest susceptibility (43.33%; n = 13). In contrast, vancomycin, rifampicin, linezolid, and daptomycin were 100% susceptible. Further disc diffusion study revealed resistance to tetracycline (33.33%), erythromycin (16.66%), and gentamicin (10.00%). The <i>tetK</i> gene was found in 33.33% of wild bird samples, while the <i>ermA</i> gene was found in 16.66% of samples. The <i>aacA-D</i> gene was only found in three (10.00%) isolates. None of the isolates tested positive for virulence genes. In conclusion, <i>S. aureus</i> is carried by wild birds in this area, posing a potentail threat to both humans and animals.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"283-290"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization of pigeon- origin avian avulavirus-1 reveals unique substitutions in F and HN proteins.","authors":"Deepthi Balam, Ratnamma Doddamane, Ramani Pushpa Rn, Shrikrishna Isloor, Veeregowda Bm","doi":"10.30466/vrf.2023.2004035.3904","DOIUrl":"10.30466/vrf.2023.2004035.3904","url":null,"abstract":"<p><p>Since decades, Newcastle disease (ND) has become endemic in the poultry population of the Indian subcontinent. ND is a highly contagious disease of poultry and other avian species. However, the genetic nature of ND viruses circulating in the rock pigeons is unraveled. The present investigation is a part of Newcastle disease virus (NDV) surveillance in wild birds. Two velogenic NDV strains could be isolated from apparently healthy rock pigeons, thus establishing the status of carrier/reservoir host. The fusion protein cleavage site in the fusion protein has multiple basic amino acid (RRRKRF) motifs similar to velogenic isolates. Phylogenetic analysis based on complete fusion gene sequences confirmed that the isolates belong to NDV sub genotype XIII 2.2. Further analysis revealed several amino acid substitutions in the hypervariable region, heptad repeat regions and neutralizing epitopes of the fusion protein and heptad repeat regions and antigenic sites of the hemagglutinin-neuraminidase (HN) protein that are critical for fusion. A unique D170A substitution in the neutralizing epitope is identified that is critical for structure and function of the fusion protein. Mutations within the virulence determinants including fusion (F) and HN, elucidate continuous evolution of the viruses among the rock pigeons. Accidental spillover of these mutated viruses into commercial poultry operations may result in disease outbreaks with economic breakdown.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"317-323"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of captopril on paraplegia caused by spinal cord ischemia-reperfusion injury in rats.","authors":"Bahareh Hafezi, Hossein Kazemi Mehrjerdi, Amir Moghaddam Jafari","doi":"10.30466/vrf.2024.2019729.4126","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019729.4126","url":null,"abstract":"<p><p>This study investigated the effect of captopril (Cap) on spinal cord ischemia-reperfusion injury (SCII) in rats. Twenty-four adults male Wistar rats were randomly divided into four groups of six animals each: spinal cord ischemia-reperfusion (SCI-R) with Cap (SCI-R + Cap), SCI-R, sham-operated with Cap (SHAM + Cap), and SHAM. The 24 hr and 90 min before ischemia induction, Cap was administered intragastrically (100 mg kg^-1) to the SHAM + Cap and SCI-R + Cap groups. Abdominal aortic clamping was performed in the SCI-R and SCI-R + Cap groups for 40 min. Hindlimb motor function was evaluated using the Tarlov Scale at 4, 6, 12, 24, 48, and 60 hr after SCII. The malondialdehyde (MDA), the ferric-reducing ability of plasma (FRAP) and prooxidant-antioxidant balance (PAB) values were also measured. Throughout the study period, the SCI-R group had significantly lower motor function scores compared to the other groups. The MDA and PAB levels were higher and the FRAP value was lower in the SCI-R group compared to in the SHAM group. The SCI-R + Cap had higher motor function scores compared to the SCI-R group at all time points. There were no significant differences in MDA concentration, FRAP and PAB values between the SCI-R + Cap and SCI-R groups. Captopril may act as a protective agent against SCII in rats based on hind limb motor function assessment.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"379-385"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}