Veterinary Research Forum最新文献_第8页

Effect of crocin on experimental gastrocnemius muscle ischemia/reperfusion injury in rat. 藏红花素对实验性大鼠腓肠肌缺血再灌注损伤的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.548566.3383

Betul Apaydin Yildirim, Selcuk Albayrak

{"title":"Effect of crocin on experimental gastrocnemius muscle ischemia/reperfusion injury in rat.","authors":"Betul Apaydin Yildirim, Selcuk Albayrak","doi":"10.30466/vrf.2022.548566.3383","DOIUrl":"https://doi.org/10.30466/vrf.2022.548566.3383","url":null,"abstract":"Ischemia-reperfusion (IR) injury to the lower extremities causes damage to various tissues, notably the limbs. Because research in recent years have demonstrated that saffron and its components are useful in ischemic strokes, the goal of this study was to see whether Crocin (Cr), one of the active constituents in saffron, could protect the gastrocnemius muscle from IR injury. A total number of 32 Sprague-Dawley rats were randomized into four groups randomly: control, Cr, IR, and IR + Cr. Xylazine and ketamine were used to anesthetize all of the rats. The left lower limbs of the other two groups were subjected to 2 hr of ischemia and 2 hr of reperfusion with tourniquet, with the exception of the control and Cr groups. Tumor necrosis factor alfa (TNF-α), interleukin 6 (IL-6), IL-1β, total antioxidant status (TAS) and total oxidant status (TOS) levels were assessed in the blood as well as muscle IL-6, IL1β, SOD1-2, catalase (CAT) and glutathione peroxidase (GPx) expression. According to the IR group, increases in TAS levels and decreases in TNF-α, IL-6, and IL-1β levels were substantial in the Cr therapy group. Cr significantly reduced IL-6 and IL-1β mRNA expression levels in the muscle of the IR group and increased superoxide dismutases 1 (SOD1), SOD2, catalase (CAT), and GPx. Our data showed that Cr protected the gastrocnemius muscle from IR injury in rats and reduced inflammatory markers significantly. These effects of Cr might have been mediated by improved antioxidant enzyme activity, suppression of free radical generation and reduction of oxidative stress.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/67/vrf-14-201.PMC10170470.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9473970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ovariohysterectomy on queen myocardial function: echocardiographic evidence. 卵巢子宫切除术对王后心肌功能的影响：超声心动图证据。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-09-15 DOI: 10.30466/vrf.2022.552942.3456

Boshra Elyasi, Mohammad Nasarolahzade Masouleh, Alireza Jahandideh

{"title":"Effect of ovariohysterectomy on queen myocardial function: echocardiographic evidence.","authors":"Boshra Elyasi, Mohammad Nasarolahzade Masouleh, Alireza Jahandideh","doi":"10.30466/vrf.2022.552942.3456","DOIUrl":"10.30466/vrf.2022.552942.3456","url":null,"abstract":"Ovariohysterectomy (OHE) is one of the most common surgeries in veterinary medicine. Although this surgical method has several advantages, it can alter the function of various organs such as heart. The present study investigated the effect of OHE on cardiac functions using M-mode echocardiography. A total of 10 healthy adult domestic short-haired cats were enrolled in the current study. Fractional shortening (FS) and ejection fraction (EF) percentages along with cardiac output (CO) were measured through the right parasternal approach in papillary muscle level view. Moreover, the levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by enzyme-linked immunosorbent assay before OHE (D0) as well as 10 (D10), 20 (D20) and 30 (D30) days after OHE. The results of this study showed that the mean FS and EF decreased on all days of the study. The FS reduction was significant between D10 and D30 and EF changes were significant between D10 and D20. The means of CO increased significantly on D0 compared to the D10. After D20, CO reduced until the end of the study. Mean concentrations of LH and FSH increased on all research days; but, the changes were significant until D20. Despite the negative effects of OHE on myocardial function, there was no significant correlation between hormonal levels and echocardiographic findings after OHE in this study.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/42/vrf-14-495.PMC10560328.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular evaluation of sheep and goats isolates of Pasteurella multocida and their antibiotic resistance. 绵羊和山羊多杀性巴氏杆菌分离株及其耐药性的分子评价。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-09-15 DOI: 10.30466/vrf.2022.556438.3524

Mohammad Tabatabaei, Fatemeh Abdolahi

{"title":"Molecular evaluation of sheep and goats isolates of Pasteurella multocida and their antibiotic resistance.","authors":"Mohammad Tabatabaei, Fatemeh Abdolahi","doi":"10.30466/vrf.2022.556438.3524","DOIUrl":"10.30466/vrf.2022.556438.3524","url":null,"abstract":"Pasteurella multocida exists as a commensal in the upper respiratory tracts of livestock, and poultry, and causes a wide variety of diseases in humans and animals. This study aimed to investigate the incidence of P. multocida by bacteriological and molecular characterization in sheep and goats and screening the existence of capsule-specific genes and their antibiotic resistance pattern. Totally, 1650 nasopharyngeal swabs were collected from apparently healthy sheep and goats and 460 lung tissues were collected from slaughtered animals in Fars province, Iran. All samples were cultured and suspected colonies were examined by biochemical tests, antimicrobial assay and polymerase chain reaction (PCR). Among 165 P. multocida (104 sheep and 61 goats) isolates, the capA, capD, and capB genes were amplified in 98, 48, and 12 isolates, respectively. The occurrence of four virulence-associated genes of P. multocida isolates were determined by PCR. Most isolates harbored the toxA (79.40%) and hgbB genes (70.90%) and 59.40% of isolates had the pfhA gene. Almost half of the isolates (46.10%) contained the tbpA gene. According to the current study, P. multocida capsular type A had the most frequency followed by type D. In addition, the high frequency of tbpA, pfhA, toxA, and hgbB genes revealed that these genes are possibly important in the pathogenesis of P. multocida. Oxytetracycline, enrofloxacin, florfenicol, and tilmicosin were the most effective drugs.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/80/vrf-14-481.PMC10560327.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of clinical and diagnostic imaging findings of bilateral superficial digital flexor tendon luxation in the tarsus of a gelding. 评估双侧指浅屈肌腱脱位的临床和诊断影像学表现。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 Epub Date: 2023-10-15 DOI: 10.30466/vrf.2023.1989381.3780

Amin Anoushepour, Saeideh Eftekhari, Majid Masoudifard

{"title":"Evaluation of clinical and diagnostic imaging findings of bilateral superficial digital flexor tendon luxation in the tarsus of a gelding.","authors":"Amin Anoushepour, Saeideh Eftekhari, Majid Masoudifard","doi":"10.30466/vrf.2023.1989381.3780","DOIUrl":"https://doi.org/10.30466/vrf.2023.1989381.3780","url":null,"abstract":"Superficial digital flexor tendon (SDFT) plays an important role in the locomotion of the horses with flexion of the limbs. In the hind limb, it passes over the calcaneal bone and attaches to medial and lateral surfaces of calcaneous by two bands of connective tissue; while, the medial band is weaker than the lateral one. Occasionally, severe trauma, over-extension or fracture of the calcaneous may cause rupture of the supportive bands leading to tendon luxation. An 11-year-old Thoroughbred jumping gelding with symptoms of lameness in both legs, after examination by an internal specialist veterinarian in equine practice field was referred to the Teaching and Research Hospital of the Faculty of Veterinary Medicine of the University of Tehran, Tehran, Iran. Clinical examination showed lateral luxation of SDFT in the right and left tarsal joints. Radiography revealed no sign of calcaneal involvement, except soft tissue swelling. In the radiography of the tarsal joints, the calcaneus bones did not show significant changes, but among the bones of the central and 3rd tarsal bone (distal intertarsal joint) of the tarsus, osteophyte formation was observed to a mild extent, indicating mild bone spavin in both tarsal joints. In ultrasonography, SDFT thickenings accompanied by hypoechoic regions in tendons were seen, confirming the signs of tendonitis. Moreover, fibrous tissue formation was diagnosed. In the left leg, anechoic fluid in the calcaneous bursa was seen. Evaluation of diagnostic imaging findings enables veterinarians to diagnose the site and extent of the lesions, choosing the appropriate therapeutic approach in addition to clinical symptoms.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71414298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review and meta-analysis of the use of plant essential oils and extracts in the development of antimicrobial edible films for dairy application. 植物精油和提取物在乳品用抗菌食用膜开发中的应用的系统综述和荟萃分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.1986122.3730

Houshmand Sharafi, Mehran Moradi, Kiomars Sharafi

{"title":"A systematic review and meta-analysis of the use of plant essential oils and extracts in the development of antimicrobial edible films for dairy application.","authors":"Houshmand Sharafi, Mehran Moradi, Kiomars Sharafi","doi":"10.30466/vrf.2022.1986122.3730","DOIUrl":"https://doi.org/10.30466/vrf.2022.1986122.3730","url":null,"abstract":"The purpose of this review was to assess the use of plant essential oils and extracts (PEOE) in the development of antimicrobial edible films for dairy application through a systematic review and meta-analysis. All studies published in multiple databases were explored via PRISMA protocol on November 1, 2022. According to the results, the interquartile range of pathogen reduction potential of essential oil (EO) in dairy products, irrespective of EO, film and product type, was 0.10 - 4.70 log CFU g-1 per % concentration. The findings from 38 articles indicate that among all EOs or their compounds, Zataria multiflora Boiss in protein film, thyme in protein film, Z. multiflora Boiss EO in protein film, Trans-cinnamaldehyde in carbohydrate film and lemongrass EO in protein film had extraordinary pathogen reduction potential on important foodborne pathogens. In the case of plant extract, fish gelatin film with Lepidium sativum extract, whey protein isolate film loaded with oregano EO and carboxymethyl cellulose film with clove EOs had the highest antimicrobial effect on mesophilic bacteria (9.50 log CFU g-1 per % concentration), yeast-mold (2.63 log CFU g-1 per % concentration) and mesophilic/ psychrophilic counts (> 9.06 log CFU g-1 per % concentration), respectively. Listeria monocytogenes is the primary species of interest; whereas, mesophiles and mold-yeast populations were the most investigated microbiota/mycobiota in cheese with PEOE-incorporated film. In light of these findings, the choice of PEOE at appropriate concentrations with the selection of appropriate edible film may improve the safety, sensory, and shelf life of dairy products.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/e1/vrf-14-179.PMC10170464.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9468686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen. 添加蒺藜水提物对犬精液冷藏过程中精子特性及抗氧化状态的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.546509.3347

Mohammad Moein Sabzeie, Esmail Ayen, Ali Soleimanzadeh, Mustafa Numan Bucak

{"title":"Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen.","authors":"Mohammad Moein Sabzeie, Esmail Ayen, Ali Soleimanzadeh, Mustafa Numan Bucak","doi":"10.30466/vrf.2022.546509.3347","DOIUrl":"https://doi.org/10.30466/vrf.2022.546509.3347","url":null,"abstract":"The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The current study looks at the possible advantages of chilling canine sperm to 4.00 ˚C for three days using Tribulus terrestris aqueous extract (TTAE). Three mixed-breed dogs were utilized to extract 24 ejaculates, which were then diluted in a Tris-based extender. The ejaculates were then divided into five groups including 20.00, 40.00 and 50.00 µg mL-1 of TTAE, sham (distilled water devoid of TTAE) and control (without TTAE) groups. During the three days of experiment, several parameters were measured every 24 hr. It was noticed that after 48 and 72 hr of liquid storage, total and progressive motilities were greater in the group with the 40.00 µg mL-1 TTAE concentration than the control group. Compared to the control group, the group with the 40.00 µg mL-1 TTAE concentration exhibited superior motility and viability. The percentages obtained from the hypo-osmotic swelling test were much greater. In contrast to the control group, DNA integrity was poorer in the 40.00 µg mL-1 TTAE concentration. After 72 hr of storage, the group with 40.00 µg mL-1 TTAE concentration had lower malondialdehyde levels but considerably greater total anti-oxidant capacity, superoxide dismutase, glutathione peroxidase and catalase levels than the control groups. The current study found that supplementing the semen extender with 40.00 µg mL-1 TTAE improves semen parameters after 72 hr of storage at 4.00 ˚C, and therefore can improve fertilization efficiency.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/8a/vrf-14-71.PMC10003598.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9470069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of intracerebroventricular injection of apelin-13 on food intake in broiler chicks. 脑室注射apelin-13对肉鸡采食量的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.545646.3335

Razieh Amini Zadeh, Hossein Jonaidi, Saeed Esmaeili Mahani, Mahsa Salehi, Mojtaba Emam Bakhsh

{"title":"Effects of intracerebroventricular injection of apelin-13 on food intake in broiler chicks.","authors":"Razieh Amini Zadeh, Hossein Jonaidi, Saeed Esmaeili Mahani, Mahsa Salehi, Mojtaba Emam Bakhsh","doi":"10.30466/vrf.2022.545646.3335","DOIUrl":"https://doi.org/10.30466/vrf.2022.545646.3335","url":null,"abstract":"Apelin is an endogenous peptide ligand for G protein coupled apelin receptors (APJ orphan receptors) which are very similar to angiotensin II receptors. Apelin is expressed in most tissues of the body including hypothalamus that is responsible for regulating water and food intake, the gastrointestinal tract, the circulatory system, adipose and muscle tissues, and the immune system. The physiological actions of apelin, including food intake, has not yet been reported in birds. In this study, the effect of intracerebroventricular injection of different doses of apelin-13 was investigated on food intake in neonatal broilers at the age of five and seven days. The chicks had access to food immediately after injection and cumulative food intake was measured at half, 1, 2, 3, 4, 8 and 21 hr after injection. The 2-way ANOVA analyzed data showed that apelin-13 at dose of 1.00 μg significantly reduced food intake at 21 hr after injection in five-day old chicks. In addition, in dose of 1.50 μg, it could significantly reduce food intake at 2, 3, 4, 8 and 21 hr after injection. In seven-day-old chicks, the doses of 1.00 and 4.00 μg of apelin-13 had no effect on food intake compared to the control group. Apelin-13 at dose of 2.00 μg significantly reduced food intake at 8 and 21 hr after injection. The results of this study showed that apelin-13 had a reducing effect on food consumption in neonatal broiler chicks.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/b8/vrf-14-105.PMC10003593.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9100410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Configuration of sacral plexuses in Hasmer and Hasak sheep breeds: a comparative macro-anatomical approach. Hasmer和Hasak羊品种的骶神经丛结构:比较宏观解剖学方法。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2021.539003.3229

Hulya Balkaya, Zekeriya Ozudogru, Dervis Dervis, Bumin Emre Teke, Hulya Kara

{"title":"Configuration of sacral plexuses in Hasmer and Hasak sheep breeds: a comparative macro-anatomical approach.","authors":"Hulya Balkaya, Zekeriya Ozudogru, Dervis Dervis, Bumin Emre Teke, Hulya Kara","doi":"10.30466/vrf.2021.539003.3229","DOIUrl":"https://doi.org/10.30466/vrf.2021.539003.3229","url":null,"abstract":"The aim of this study was to examine and compare the macro-anatomical structures and configurations of plexus sacralis in Hasmer and Hasak sheep breeds. For this purpose, 18 sheep (eight female Hasmer sheep, three male Hasak sheep and seven female Hasak sheep) were assessed. After induction of anesthesia, the body cavities were opened. The sheep were fixed with formaldehyde after draining of the blood. The nerves of the plexus sacralis were dissected separately and photographed. Both in Hasmer and Hasak sheep breeds, the plexus sacralis was a large anastomotic complex of nerves which gave rise to nerves distributed to pelvic cavity and pelvic limb. It was derived from the ventral branches of the six spinal segments. It was observed that this plexus was formed by the ventral rami of four (first to fourth) sacral spinal nerves as well as ventral ramus of the sixth and fifth lumbar nerves. The results of this study are believed to be improved further with the future morphometric studies and to make a contribution to future anatomical and surgical studies in this area.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/93/vrf-14-65.PMC10003599.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9100416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational design of a chimeric toxin against Claudin-4-expressing cancer cells: molecular modeling, docking and molecular dynamics simulation analysis. 针对表达claudin -4的癌细胞嵌合毒素的计算设计:分子建模、对接和分子动力学模拟分析。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.548415.3378

Sepehr Safaei, Mehdi Imani

{"title":"Computational design of a chimeric toxin against Claudin-4-expressing cancer cells: molecular modeling, docking and molecular dynamics simulation analysis.","authors":"Sepehr Safaei, Mehdi Imani","doi":"10.30466/vrf.2022.548415.3378","DOIUrl":"https://doi.org/10.30466/vrf.2022.548415.3378","url":null,"abstract":"Cancer is one of the main reasons of mortality all over the world. Over the time, the major ways for cancer-therapy were based on radiotherapy, chemotherapy and surgery. These methods are not specific enough for that purpose, therefore, new ideas for design of new drugs with higher specificity are considered. Chimeric protein toxins are hybrid proteins consisting of a targeting portion and a toxic one which specifically bind and kill the target cancer cells. The main purpose of this study was designing a recombinant chimeric toxin with biding capability to one of the most key receptors namely claudin-4 which is over-expressed in almost all cancer cells. To design it, we utilized the last 30 C-terminal amino acids of Clostridium perfringens enterotoxin (CPE) as a binding module for claudin-4 and the toxic module which is the A-domain of Shiga toxin from Shigella dysenteriae. Using molecular modeling and docking methods, appropriate binding affinity of the recombinant chimeric toxin to its specific receptor was demonstrated. In the next step, the stability of this interaction was investigated by molecular dynamics simulation. Although partial instability was detected at some time points, however, sufficient stable situation of hydrogens bonds and high binding affinity between the chimeric toxin and receptor were observed in the in silico studies which in turn suggested that this complex could be formed successfully.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/1e/vrf-14-259.PMC10278906.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10067558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echocardiographic assessment of right ventricular blood flow and tricuspid annular plane systolic excursion in apparently healthy domestic short-haired cats. 超声心动图评价健康家养短毛猫右心室血流和三尖瓣环面收缩偏移。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2023-01-01 DOI: 10.30466/vrf.2022.548794.3387

Zahra Darabizadeh, Keivan Keramati, Daruish Shirani, Mohammad Molazem, Saeed Heydaryan, Yasamin Vali

{"title":"Echocardiographic assessment of right ventricular blood flow and tricuspid annular plane systolic excursion in apparently healthy domestic short-haired cats.","authors":"Zahra Darabizadeh, Keivan Keramati, Daruish Shirani, Mohammad Molazem, Saeed Heydaryan, Yasamin Vali","doi":"10.30466/vrf.2022.548794.3387","DOIUrl":"https://doi.org/10.30466/vrf.2022.548794.3387","url":null,"abstract":"Right ventricular parameters change in many cardiovascular diseases; so, the presence of normal right ventricular parameters is necessary to diagnose these diseases. Ten clinically healthy adult domestic short-haired cats including six males and four females in the range of 2.70 to 4.80 kg were studied using echocardiography without sedation. The speed and pressure of blood flow through the tricuspid and pulmonary valves, speed of the movement of tricuspid valve and tricuspid annular plane systolic excursion (TAPSE) were recorded using conventional pulsed-wave (PW) Doppler, tissue Doppler imaging (TDI) and M-mode, respectively. No significant statistical differences were observed between the measured values and sex, heart rate and body weight. A positive correlation was observed between the maximum velocity of the right ventricular outflow tract and heart rate and also between TAPSE slope and body weight. With determining the normal values of PW-TDI of the right ventricle in apparently healthy domestic short-haired cats, it is expected that with providing normal reference values, the diagnosis of heart diseases especially asymptomatic heart disease be achieved as soon as possible to provide the best appropriate therapeutic management and monitoring decision about them.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/fb/vrf-14-275.PMC10278901.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9701023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0