Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-05-15DOI: 10.30466/vrf.2023.2014356.4044
Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani
{"title":"Anti-<i>Listeria</i> activity of postbiotics of <i>Lactiplantibacillus</i> <i>sakei</i> in beef fillet using aerosolization technique.","authors":"Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani","doi":"10.30466/vrf.2023.2014356.4044","DOIUrl":"10.30466/vrf.2023.2014356.4044","url":null,"abstract":"<p><p>In recent years, the use of probiotics and their metabolites, known as postbiotics as natural preservatives has received increasing attention in the food industry. This study aimed to prepare and characterize postbiotics of <i>Lactiplantibacillus sakei</i> and to investigate its application as an anti-<i>Listeria</i> solution on beef fillets using an aerosolization technique. The functional groups, including organic acids, polysaccharides and other minor metabolites, were identified by Fourier transform infrared (FTIR) in the postbiotics. The 2, 2'-diphenyl-1-picrylhydrazyl radical scavenging activity of the postbiotics was reported as 0.82 mg mL<sup>-1</sup>. The antimicrobial test using the agar well diffusion method revealed a zone of inhibition of 27.00 ± 1.20 mm. Application of an aerosolized postbiotics solution resulted in a significant reduction in <i>Listeria monocytogenes</i> counts on beef fillets, reaching 3.30 log<sub>10</sub> CFU g<sup>-1</sup> over a 15-day storage period at 4.00 ± 1.00 ˚C. The results of this study revealed that the postbiotics of <i>L. sakei</i> was an effective antimicrobial additive for controlling foodborne pathogens in beef fillets and aerosolization is a promising method for developing an antimicrobial coating on meat to enhance meat safety.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"223-229"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-04-15DOI: 10.30466/vrf.2024.2020553.4141
Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk
{"title":"Congenital feline tuberculosis: the first case report.","authors":"Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk","doi":"10.30466/vrf.2024.2020553.4141","DOIUrl":"10.30466/vrf.2024.2020553.4141","url":null,"abstract":"<p><p>This study presents the first report of congenital tuberculosis in an 8-month-old male British Shorthair cat. The case was examined using histopathological and immunohistochemical methods. The cat was referred to a private veterinary clinic with general respiratory system problems and subsequent deterioration, leading to death. The cat owner granted permission for the cat necropsy and pathological examinations at Department of Pathology, Faculty of Veterinary Medicine, Selçuk University, Konya, Türkiye. During systemic necropsy, white round foci with diameters ranging from 3.00 to 5.00 mm were observed in the lung and spleen. Tissue samples were collected from the lung, spleen, liver, heart, kidney, mediastinal lymph nodes and brain for histopathological examinations. The tissues were subjected to routine histological tissue processing and sections were stained with Hematoxylin and Eosin and Ziehl-Neelsen. Additionally, <i>Mycobacterium spp</i>. antibodies were used for immunohistochemical staining. Microscopic examination revealed exudative tuberculosis lesions, areas of necrosis without a fibrous capsule and karyorrhectic cells only in the lung and spleen. Acid-resistant bacteria observed by ZN staining in the lesioned areas of the lung and spleen were identified as <i>Mycobacterium spp</i>. using immunohistochemical staining. No positive staining was observed in other organs using ZN and immunohistochemical methods. As a result, congenital tuberculosis was diagnosed in a cat for the first time, especially in relation to lesions in the spleen.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"203-205"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of thymoquinone on acetic acid-induced visceral nociception in rats: role of central cannabinoid and α<sub>2</sub>-adrenergic receptors.","authors":"Somayyeh Naderi, Esmaeal Tamaddonfard, Saeid Nafisi, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2023.2005364.3922","DOIUrl":"10.30466/vrf.2023.2005364.3922","url":null,"abstract":"<p><p>Thymoquinone (TQ) is the main biologically active substance of <i>Nigella sativa</i> (black seeds). It has anti-cancer, anti-inflammatory, anti-diabetic, anti-oxidative and anti-nociceptive properties. This study was aimed to explore the effect of TQ on acetic acid-induced visceral nociception. The central mechanisms of the effect of TQ were investigated using cannabinergic (AM251) and α<sub>2</sub>-adrenergic (yohimbine [Yoh]) antagonists. The lateral ventricle of the brain was cannulated for intracerebroventricular (ICV) injections. Visceral nociception was induced by intra-peritoneal (IP) injection of acetic acid (1.00% in a volume of 1.00 mL). Measuring the latency time to the first writhing appearance and counting the number of writhing in 5-min intervals for a period of 60 min were performed. Locomotor activity was determined using an open-field test. Oral administration (PO) of 2.50 and 10.00 mg kg<sup>-1</sup> TQ increased the latency time to the first writhing appearance and decreased the number of writhing. The AM251 (5.00 µg <i>per</i> rat; ICV) and Yoh (5.00 µg <i>per</i> rat; ICV) partially prevented TQ (10.00 mg kg<sup>-1</sup>; PO)-induced anti-nociception. Locomotor activity was not altered by these treatments. The results of the present study showed that TQ had the ability to reduce visceral nociception caused by IP injection of acetic acid. The central mechanisms of this action of TQ might be partially mediated by cannabinergic and α<sub>2</sub>-adrenegic receptors.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"131-138"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic characterization of virulence and extended spectrum β-lactamase producing genes of <i>Klebsiella pneumoniae</i> isolated from bovine milk.","authors":"Bhavinkumar Pankajbhai Katira, Bhaveshkumar Ishwarbhai Prajapati, Ratn Deep Singh, Sandipkumar Sureshbhai Patel, Kirankumar Motiji Solanki","doi":"10.30466/vrf.2023.2006974.3947","DOIUrl":"10.30466/vrf.2023.2006974.3947","url":null,"abstract":"<p><p>Mastitis associated <i>Klebsiella pneumoniae</i> species were isolated from bovine milk to characterize virulence genes (<i>wabG</i> and <i>kfuBC</i>) and extended spectrum β-lactamase (ESBL) genes (<i>bla</i><sub>CTX-M-1</sub>, <i>bla</i><sub>CTX-M-2</sub>, <i>bla</i><sub>CTX-M-9</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>SHV</sub> and <i>bla</i><sub>OXA</sub>). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 <i>K. pneumoniae</i> isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of <i>wabG</i> (25/27; 92.59%) was higher than <i>kfuBC</i> (5/27; 18.51%). Beta-lactamase genes <i>viz., bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>CTX-M-1</sub> were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for <i>bla</i><sub>CTX-M-9</sub> and <i>bla</i><sub>OXA-1</sub> genes. Outcome of the study provided an insight into virulence genes and ESBL producing <i>K. pneumoniae</i> isolated from bovine milk samples in India.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"57-64"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-02-15DOI: 10.30466/vrf.2023.2000526.3855
Ahmad Enferadi, Abdolghaffar Ownagh, Mousa Tavassoli
{"title":"Molecular detection and phylogenetic analysis of <i>Borrelia</i> spp. from sheep and goats blood samples in West Azerbaijan province, Iran.","authors":"Ahmad Enferadi, Abdolghaffar Ownagh, Mousa Tavassoli","doi":"10.30466/vrf.2023.2000526.3855","DOIUrl":"10.30466/vrf.2023.2000526.3855","url":null,"abstract":"<p><p><i>Borrelia</i> species are spirochetes transmitted by ticks that are important in human and animals. In most countries, there is still no molecular epidemiology of borreliosis in ruminants. This study was aimed to evaluate the existence of <i>Borrelia</i> spp. DNA in the blood samples of small ruminants using polymerase chain reaction (PCR) method in West Azerbaijan Province, Iran. To detect <i>Borrelia</i> spp. DNA, about 1,018 ruminants (456 goats and 562 sheep) blood samples were examined from different bioclimatic regions in West Azerbaijan province, Iran. The DNA extracting and PCR were conducted. In sheep, the following prevalence rates were respectively obtained for the <i>16S rRNA, 5S - 23S rRNA</i> and <i>ospA</i> genes: 3.55% (20/562), 2.13% (12/562) and 0.88% (5/562). And so, the prevalence rates of the genes in goats were 0.87% (4/456) for <i>5S - 23S rRNA</i> gene, 1.75% (8/456) for <i>16S rRNA</i> gene and 0.65% (3/456) for <i>ospA</i> gene. The prevalence of <i>Borrelia</i> spp. was significantly different in small ruminants based on the farms and localities. The sheep and goats in humid areas (north of West Azerbaijan) were infected statistically more than those in sub-humid areas (south of West Azerbaijan). It is demonstrated that host species like sheep and goats may have a key role in natural Lyme disease cycles and other borreliosis diseases in Iran.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"89-95"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Temporal alteration in ovarian follicle fate with hormonal and hemodynamic perspectives during receptive phase of estrous cycle in endometritic cattle.","authors":"Akshay Sharma, Pravesh Kumar, Pankaj Sood, Vijender Negi, Pururava Sharma","doi":"10.30466/vrf.2023.2013227.4033","DOIUrl":"10.30466/vrf.2023.2013227.4033","url":null,"abstract":"<p><p>The present research was carried out to assess the serum progesterone (P<sub>4</sub>) concentration and uterine hemodynamics at estrus till ovulation in cyclic cows (N = 130) with healthy or diseased uterus. At estrus, 85 cows were diagnosed with clinical endometritis (CE; n = 44) and sub-clinical endometritis (SCE; n = 41); whereas, 45 cows being served as control namely no endometritis (NE; n = 45) were included in the study. Serum progesterone estimation at 12 - 14 and 40 hr after the onset of estrus and Doppler sonography of both middle uterine arteries were done to envisage the uterine hemodynamics and ovulation. The serum progesterone concentration was significantly higher at 12 - 14 hr after onset of estrus in CE and SCE cows. At 12 - 14 hr after onset of estrus, a cut-off value of ≥ 0.48 ng mL<sup>-1</sup> P<sub>4</sub> was obtained, above which 22.72% CE, 26.82% SCE and only 8.88% NE cows failed to ovulate within 36 - 40 hr of estrus onset. Among the Doppler indices, pulsatility and resistance indices were significantly higher; whereas, volume and velocity indices were significantly lower in NE cows. In cows diagnosed with CE and SCE, a higher supra-basal P<sub>4</sub> concentration, and velocity and volume of blood flow to uterus at estrus negatively affected the duration to ovulation.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"83-88"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2023.2000166.3851
Sina Tamaddonfard, Amir Erfanparast, Esmaeal Tamaddonfard, Farhad Soltanalinejad
{"title":"The CB1 cannabinoid receptors involvement in anti-epileptic effect of safranal on penicillin-induced epileptiform activity in rats.","authors":"Sina Tamaddonfard, Amir Erfanparast, Esmaeal Tamaddonfard, Farhad Soltanalinejad","doi":"10.30466/vrf.2023.2000166.3851","DOIUrl":"10.30466/vrf.2023.2000166.3851","url":null,"abstract":"<p><p>Neuroprotective effects for natural products are supported by several studies. In this regard, safranal, a constitute of saffron, has the potential to exert beneficial effects in neuro-logical disorders such as Parkinson's disease, epilepsy, stroke, multiple sclerosis and Alzheimer's disease. Here, we investigated the effect of safranal on penicillin-induced epileptiform activity. Also, the effects of intracerebroventricular (ICV) microinjection of AM251 as a CB1-cannabinoid receptors antagonist to clarify the possible mechanism of safranal were evaluated. Epileptiform activity was induced by intra-cortical administration of penicillin (300 IU, 1.50 μL) in urethane-anesthetized rats. Electrocorticographic recordings were used to analyze the frequency and amplitude of spike waves. Intraperitoneal injections of safranal at doses of 1.00 and 4.00 mg kg<sup>-1</sup> significantly reduced both the number and amplitude of spike waves. The ICV microinjection of AM251 (0.50 μg 2.00 μL<sup>-1</sup>) significantly increased the frequency and amplitude of spike waves. In addition, the anti-epileptic effect induced by administration of safranal at a dose of 4.00 mg kg<sup>-1</sup> was partially prevented by ICV microinjection of 0.50 μg 2.00 μL<sup>-1</sup> of AM251. The results showed anti-epileptiform activities for safranal. Central CB1 cannabinergic receptors might be involved in the anti-epileptiform activity of safranal.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"35-41"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2022.554037.3474
Lucai Wang, Lijia Liu, Huanrong Zhang
{"title":"Development and validation of an insulated isothermal polymerase chain reaction assay for the rapid detection of <i>Mycoplasma synoviae</i>.","authors":"Lucai Wang, Lijia Liu, Huanrong Zhang","doi":"10.30466/vrf.2022.554037.3474","DOIUrl":"10.30466/vrf.2022.554037.3474","url":null,"abstract":"<p><p><i>Mycoplasma synoviae</i>, which causes the disease known as chicken synovitis, causes serious immunosuppression. We developed a rapid insulated isothermal polymerase chain reaction (iiPCR) assay for on-site detection of <i>M. synoviae</i> using a primer and probe set targeting the <i>variable lipoprotein and haemagglutinin</i> (<i>vlh</i><i>A</i>) gene. In addition, the specificity, sensitivity, repeatability, and clinical detection of this method were evaluated. Our iiPCR assay detected <i>M. synoviae</i> clinical isolates and samples successfully and produced negative results on <i>Mycoplasma galliscepticum</i><i>,</i> avian viral arthritis, <i>Escherichia coli</i>, <i>Salmonella</i>, <i>Staphylococcus aureus</i> and <i>Corynebacterium</i>, indicating that the PCR reactions were specific. Additionally, our iiPCR assay detected the prepared positive standard plasmid diluted 10 times (1.00 × 10<sup>-1</sup> - 1.00 × 10<sup>-10</sup>) as a template. The undiluted positive plasmid was positive and double distilled water was negative indicating that the PCR reactions were sensitive, respectively. Finally, the <i>vlhA</i> positive standard plasmid with dilution multiple of 1.00 × 10<sup>-4</sup> - 1.00 × 10<sup>-6</sup> was repeatedly detected three times to evaluate the repeatability of the iiPCR method established in this experiment showing that the iiPCR of <i>M. synoviae</i> is repeatable. The established iiPCR was also used to detect 50 chicken joint enlargement samples. The thermostatic detection PCR established in this experiment was comparable to a reference real-time PCR (qPCR).</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"7-12"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-05-15DOI: 10.30466/vrf.2023.1999287.3836
Zahra Amini, Fatemeh Hoseinpour, Ali Ghashghaii, Hadi Cheraghi
{"title":"An analysis of oxidative stress indices and clinical parameters in budgerigars (<i>Melopsittacus undulatus</i>) treated with medetomidine-ketamine and midazolam-ketamine.","authors":"Zahra Amini, Fatemeh Hoseinpour, Ali Ghashghaii, Hadi Cheraghi","doi":"10.30466/vrf.2023.1999287.3836","DOIUrl":"10.30466/vrf.2023.1999287.3836","url":null,"abstract":"<p><p>Various companion birds, including budgerigars, are anesthetized with injectable anesthesia. The current study aimed to evaluate oxidative stress indices including malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) along with clinical parameters such as the time required to induce, maintain and recover from medetomidine-ketamine anesthesia and midazolam-ketamine anesthesia in budgerigars. Among 20 mature and healthy budgerigars, three groups were assigned as follows: Control (n = 4) to determine baseline oxidative stress indices medetomidine + ketamine (n = 8) anesthetized by intramuscular injections of medetomidine (0.04 mg kg<sup>-1</sup>) and ketamine (30.00 mg kg<sup>-1</sup>) in the pectoral muscles, midazolam + ketamine (n = 8) anesthetized by intramuscular injections of midazolam (1.00 mg kg<sup>-1</sup>) and ketamine (50.00 mg kg<sup>-1</sup>). Half of birds (n = 4) in the second and third groups were euthanized by cervical dislocation 1 hr after anesthesia induction, blood samples were collected directly from the heart, and sera were extracted. Additionally, the remaining birds were euthanized 24 hr later, and their serum was analyzed for oxidative stress indices. Clinical parameters were recorded during the study. Compared to the medetomidine + ketamine group, the midazolam + ketamine group experienced shorter induction, anesthetic, and recovery times. Administering medetomidine and ketamine elevated TOS levels compared with midazolam + ketamine. No significant difference was found between the test groups for TAC, MDA, or OSI. Therefore, the midazolam + ketamine regimen appears superior to medetomidine + ketamine when performing minor surgeries on budgerigars.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"237-242"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-09-15DOI: 10.30466/vrf.2024.2020333.4136
Soheil Vazifehdoust, Ali Shalizar-Jalali, Mohammad Reza Nourani, Mehrdad Moosazadeh Moghaddam, Mohsen Yazdanian
{"title":"Improvement of osteogenesis and antibacterial properties of a bioactive glass/gelatin-based scaffold using zoledronic acid and CM11 peptide.","authors":"Soheil Vazifehdoust, Ali Shalizar-Jalali, Mohammad Reza Nourani, Mehrdad Moosazadeh Moghaddam, Mohsen Yazdanian","doi":"10.30466/vrf.2024.2020333.4136","DOIUrl":"10.30466/vrf.2024.2020333.4136","url":null,"abstract":"<p><p>This study aimed to investigate the effects of zoledronic acid (ZA) and antibacterial CM11 peptide on the osteoinduction and antibacterial properties of bioactive glass (BG). The bioactive glass/gelatin (BG/Gel) composite was synthesized using the sol-gel method. The 2-x minimum inhibitory concentration of the peptide and 4.00 mg mL<sup>-1</sup> of ZA were added to the BG/Gel during fabrication. The BG/Gel composite morphological and structural characteristics and anti-bacterial activities were analyzed using Fourier transform infra-red spectroscopy, scanning electron microscopy and disk diffusion test, respectively. The release of the peptide and ZA from BG/Gel was measured using ultra-violet spectroscopy. After 14 days, the effects of the peptide/ ZA-containing BG/Gel (PZ-BG/Gel) on the growth and differentiation of mesenchymal stem cells were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, calcium and alkaline phosphatase assays, immunocytochemical staining for osteocalcin (OCN) and real-time polymerase chain reaction for <i>OCN</i>, <i>type I collagen</i>, <i>bone morphogenetic protein 2</i> and <i>Runt-related transcription factor-2</i> genes. The disk diffusion test showed the anti-microbial activity of the scaffold against multi-drug-resistant isolates of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. Analyses showed a significantly higher level of stem cells differentiation into the osteo-genic cells in PZ-BG/Gel scaffold compared to BG/Gel scaffold alone. Accordingly, osteoblast markers were significantly increased in comparison with the control. In conclusion, the osteo-induction and antibacterial properties of BG-based scaffold can be improved using ZA and CM11.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"487-498"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}