Veterinary Research Forum最新文献_第7页

Viability of guinea pig (Cavia porcellus) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C. 用Tris-buffer扩展器在5.00℃下保存的豚鼠（Cavia porcellus）精子的活力。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2023686.4186

Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad

{"title":"Viability of guinea pig (Cavia porcellus) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C.","authors":"Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad","doi":"10.30466/vrf.2024.2023686.4186","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023686.4186","url":null,"abstract":"The cooling procedure markedly diminishes the quality of guinea pig (Cavia porcellus) sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions. This study aimed to assess the viability of guinea pig spermatozoa diluted in Tris buffer-based extenders during storage at 5.00 ˚C. Sperm collection was carried out through castration of the animals. For this study, 10 adult male guinea pigs were utilized, being divided into four groups including phosphate-buffered saline (PBS), human tubal fluid (HTF), Tris-citric-glucose (TCG), and Tris-fructose-yolk (TFY) cultures. Evaluations including sperm motility, morphology, plasma membrane integrity, viability, and total count were conducted at 0, 24, and 48 hr after sampling. The results obtained indicated that at the 24-hr and 48-hr marks of the experiment, both overall and progressive motility percentages, viability, plasma membrane integrity, and morphology of sperms in the PBS and HTF cultures exhibited a significant increase in comparison with the TCG and TFY cultures. Consequently, it can be inferred that PBS and HTF cultures are highly effective in preserving the quality of guinea pig spermatozoa.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"691-696"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. 比较乙酰丙嗪-氯硝柳胺与乙酰丙嗪-氯硝柳胺复方制剂对马的镇静和心血管作用。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2004451.3910

Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei

{"title":"Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses.","authors":"Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2023.2004451.3910","DOIUrl":"10.30466/vrf.2023.2004451.3910","url":null,"abstract":"The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg-1) in combination with acepromazine maleate (0.05 mg kg-1) intravenously (IV). In treatment II (T2), the animals received intra-gastric administration of clonidine (0.002 mg kg-1) followed by acepromazine (0.05 mg kg-1; IV) after 60 min. Head height above the ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 min before and 5, 15, 30, 60, and 90 min after drug administration. In T2, recordings were made 5 min before clonidine, 55 min after clonidine administration, and then 5, 15, 30, 60, and 90 min after acepromazine injection. Analyses of the data showed there were not significant differences regarding HHAG and echocardiographic indices between two treatments. For sedation of healthy horses, it was concluded that intra-gastric administration of clonidine and IV administration of acepromazine showed similar sedative and cardiovascular effects compared to IV acepromazine-xylazine administration.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"21-26"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system. 姜黄素对福尔马林诱导的雄性大鼠肌肉疼痛的影响：局部环氧化酶系统的作用

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2015987.4069

Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad

{"title":"Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system.","authors":"Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2024.2015987.4069","DOIUrl":"https://doi.org/10.30466/vrf.2024.2015987.4069","url":null,"abstract":"Investigating the mechanisms responsible for pain processing of natural and synthetic chemical compounds is necessary to optimize pain management. Curcumin (Cur), the active ingredient of turmeric, exhibits potent analgesic and anti-inflammatory properties by employing multiple mechanisms at the local peripheral, spinal and supra-spinal levels. This study was aimed to investigate the effect of oral administration of Cur on muscle pain induced by intramuscular (IM) injection of formalin. To explore the possible local mechanisms, a cyclooxygenase (COX) inhibitor, diclofenac (Dic) and a COX product, prostaglandin E2 (PGE2), were applied. The IM injection of formalin (25.00 µL, 2.50%) into the gastrocnemius muscle induced two distinct phases of hind leg flinching. A short-lasting (10 min) hind leg lifting was observed following IM injection of PGE2 (2 µg kg-1, 25.00 µL). Oral administration of Cur (25.00 and 100 mg kg-1) and IM injection of 40.00 µg kg-1 Dic attenuated formalin and PGE2 induced nociceptive behaviors. Contra-lateral IM injection of Dic did not change muscle pain induced by ipsilateral IM injection of formalin and PGE2. The second phase of formalin induced flinching as well as PGE2 evoked lifting were more suppressed when 40.00 µg kg-1 Dic and 100 mg kg-1 Cur were used together. Locomotor activity was not changed by the above-mentioned treatments. It was concluded that the reducing effect of muscle pain of Cur might be related to the local inhibition of COX.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"411-416"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of the beta-lactamase Cefotaxime gene in Escherichia coli from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island. 从龙目岛重复繁殖的巴厘岛牛生殖道中分子检测大肠杆菌中的β-内酰胺酶头孢他啶基因。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2014818.4056

Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati

{"title":"Molecular detection of the beta-lactamase Cefotaxime gene in Escherichia coli from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island.","authors":"Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati","doi":"10.30466/vrf.2024.2014818.4056","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014818.4056","url":null,"abstract":"This study aims to detect Escherichia coli which encodes beta-lactamase Cefotaxime (blaCTX-M) gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of E. coli was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of E. coli which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the blaCTX-M gene of E. coli and sequences of the blaCTX-M gene were phylogenetically analyzed. The research results obtained three E. coli isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of E. coli was resistant to penicillin G and oxytetracycline. 66.66% of E. coli was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of E. coli was resistant to tetracycline. Escherichia coli isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of E. coli detection using PCR showed that three E. coli isolates encoded the blaCTX-M gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that E. coli from the reproductive tract of Bali cattle was related to E. coli that encoded blaCTX-M-14 isolated from humans.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"397-402"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of Nigella sativa oil, thymoquinone and dexamethasone on bleomycin-induced lung fibrosis in rats. 黑草油、百里醌和地塞米松对博莱霉素致大鼠肺纤维化的保护作用。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2024154.4196

Farid Saghghaei, Ali Rassouli, Goudarz Sadeghi-Hashjin, Farhang Sasani, Mohammad Kazem Koohi

{"title":"Protective effects of Nigella sativa oil, thymoquinone and dexamethasone on bleomycin-induced lung fibrosis in rats.","authors":"Farid Saghghaei, Ali Rassouli, Goudarz Sadeghi-Hashjin, Farhang Sasani, Mohammad Kazem Koohi","doi":"10.30466/vrf.2024.2024154.4196","DOIUrl":"10.30466/vrf.2024.2024154.4196","url":null,"abstract":"Pulmonary fibrosis (PF) is a chronic interstitial lung disease with a progressive damage to the air sacs and deposition of collagen fibers in the lung tissue. The study aimed to explore the effects of Nigella sativa oil (NSO) or thymoquinone (TQ), alone or in combination with dexamethasone (DEX), on the development of bleomycin (BLM)-induced PF. Forty-two male rats were divided into seven groups: Control (CTRL); BLM, received a single dose of BLM on day 0, intratracheally; all remaining groups received BLM, as well. DEX, received DEX daily, intraperitoneally, 1 day before BLM and continued for 14 days; NSO and TQ groups, received daily NSO and TQ, respectively, 7 days before BLM and continued for 35 days; DEX + TQ, received both DEX and TQ; DEX + NSO, received both DEX and NSO. At the end, lung tissues were used for histopathological and biochemical analyses. BLM significantly increased the severity of fibrosis and inflammation compared to the CTRL. Bleomycin also significantly increased the amount of hydroxyproline, however, decreased most antioxidant enzymes in the lung tissue compared to the other groups. Group TQ + DEX significantly reduced the severity of BLM-induced PF as well as alterations in biochemical parameters, lung weight and O2 saturation. Nigella sativa oil slightly reduced BLM-induced PF, however, it caused non-significant hyperemia in lung tissue. Thymoquinone potentiated the effects of DEX on most biochemical and pathological alterations of BLM-induced lung injury much better than NSO. More studies are needed to support the use of NSO and TQ as potential protective agents against PF.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"613-620"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression. MicroRNA-142-3p 使乳腺癌对多西他赛化疗敏感：诱导细胞凋亡和细胞周期停滞，并抑制迁移。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-11-15 DOI: 10.30466/vrf.2024.2022533.4165

Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran

{"title":"MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression.","authors":"Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran","doi":"10.30466/vrf.2024.2022533.4165","DOIUrl":"10.30466/vrf.2024.2022533.4165","url":null,"abstract":"Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-142-3p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-142-3p was initially assessed in MDA-MB-468 cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-142-3p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-142-3p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX2, Octamer 4, HMGA2, Kruppel-like factor 4, and Bach-1. Additionally, the combination of miRNA-142-3p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G1 phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-142-3p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-142-3p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"629-643"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Brucella spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023. 伊朗工业化奶牛场布鲁氏菌属循环特征：2016 - 2023 年实地研究。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2012972.4028

Saeed Alamian, Karim Amiry, Afshar Etemadi, Maryam Dadar

{"title":"Characterization of Brucella spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023.","authors":"Saeed Alamian, Karim Amiry, Afshar Etemadi, Maryam Dadar","doi":"10.30466/vrf.2024.2012972.4028","DOIUrl":"10.30466/vrf.2024.2012972.4028","url":null,"abstract":"Bovine brucellosis, an infectious disease transmitted by Brucella melitensis and Brucella abortus, presents a significant zoonotic risk for agricultural economics and animal health. The primary objective of this study was to present a comprehensive understanding of the prevalence and features of Brucella strains within the industrial dairy farming sector in Iran. Rose Bengal plate test, standard agglutination test, and indirect enzyme linked immunosorbent assay tests were used to confirm all seropositive animals. A total number of 1,311 bovine samples from seropositive animals including were collected from 224 farms in 21 provinces of different regions of Iran and examined. The discovered Brucella isolates were phenotyped and molecularly characterized. The isolates were all B. abortus or B. melitensis. Bacteria analysis revealed that 70.53% of seropositive farms were tested positive for Brucella strains, predominantly B. melitensis biovar 1 (43.42%) and B. abortus biovar 3 (27.11%). Geographical distribution revealed that B. melitensis biovar 1 was the most common in dairy cow farms (16 provinces), followed by B. abortus biovar 3 (six provinces). Also, the prevalence of B. melitensis biovar 2, B. melitensis biovar 3, B. abortus biovar 1, B. abortus biovar 2 and RB51 vaccine were restricted to certain provinces. AMOS (abortus melitensis ovis suis)-polymerase chain reaction and Bruce-ladder PCR confirmed species identification. These results highlighted the complexity of bovine brucellosis in Iran and illustrated that B. melitensis was spread from small ruminants to cattle. This study provided important epidemiological insights for targeting future brucellosis control programs in the Iranian dairy farms.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"195-202"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generalized megaesophagus associated with Schmidt-like syndrome in a dog. 与施密特样综合征相关的犬全身巨食管症。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2011964.4014

Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei

{"title":"Generalized megaesophagus associated with Schmidt-like syndrome in a dog.","authors":"Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2011964.4014","DOIUrl":"10.30466/vrf.2024.2011964.4014","url":null,"abstract":"A 2-year-old intact male Asian Shepherd dog was referred with a history of chronic regurgitation along with normal appetite and diagnosis of megaesophagus on plain radiography. Clinical examination revealed normothermia, normocardia, normopnea, low body condition score and poor hair coat. The most important laboratory findings include anemia, azotemia, hyperlipidemia, increased thyroid stimulating hormone, decreased thyroxine and hypocortisolemia, as well as a marked increase in acetylcholine receptor antibody concentration. Based on the results, in addition to primary hypothyroidism and primary hypoadrenocorticism, myasthenia gravis was also diagnosed as an underlying cause of megaesophagus. Following nursing care and preferred treatment of each disease, the megaesophagus was resolved in the next visit. This clinical report describes for the first time, to the authors' knowledge, a dog with a rare type of autoimmune polyglandular syndrome (APS) known in human medicine as a Schmidt's syndrome. We want to emphasize the importance of clinicians' awareness regarding the possibility of APS to identify different diseases caused by it in order to achieve successful treatment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"215-218"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Listeria activity of postbiotics of Lactiplantibacillus sakei in beef fillet using aerosolization technique. 利用气溶胶技术在牛肉片中添加清酒乳杆菌后生菌的抗李斯特菌活性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.30466/vrf.2023.2014356.4044

Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani

{"title":"Anti-Listeria activity of postbiotics of Lactiplantibacillus sakei in beef fillet using aerosolization technique.","authors":"Sima Valipour, Hossein Tajik, Mehran Moradi, Rahim Molaei, Mahdi Ghorbani","doi":"10.30466/vrf.2023.2014356.4044","DOIUrl":"10.30466/vrf.2023.2014356.4044","url":null,"abstract":"In recent years, the use of probiotics and their metabolites, known as postbiotics as natural preservatives has received increasing attention in the food industry. This study aimed to prepare and characterize postbiotics of Lactiplantibacillus sakei and to investigate its application as an anti-Listeria solution on beef fillets using an aerosolization technique. The functional groups, including organic acids, polysaccharides and other minor metabolites, were identified by Fourier transform infrared (FTIR) in the postbiotics. The 2, 2'-diphenyl-1-picrylhydrazyl radical scavenging activity of the postbiotics was reported as 0.82 mg mL-1. The antimicrobial test using the agar well diffusion method revealed a zone of inhibition of 27.00 ± 1.20 mm. Application of an aerosolized postbiotics solution resulted in a significant reduction in Listeria monocytogenes counts on beef fillets, reaching 3.30 log10 CFU g-1 over a 15-day storage period at 4.00 ± 1.00 ˚C. The results of this study revealed that the postbiotics of L. sakei was an effective antimicrobial additive for controlling foodborne pathogens in beef fillets and aerosolization is a promising method for developing an antimicrobial coating on meat to enhance meat safety.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"223-229"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital feline tuberculosis: the first case report. 先天性猫结核病：首例报告。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2020553.4141

Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk

{"title":"Congenital feline tuberculosis: the first case report.","authors":"Osman Dağar, Mehmet Burak Ateş, Mustafa Ortatatlı, Fatih Mehmet Öztürk","doi":"10.30466/vrf.2024.2020553.4141","DOIUrl":"10.30466/vrf.2024.2020553.4141","url":null,"abstract":"This study presents the first report of congenital tuberculosis in an 8-month-old male British Shorthair cat. The case was examined using histopathological and immunohistochemical methods. The cat was referred to a private veterinary clinic with general respiratory system problems and subsequent deterioration, leading to death. The cat owner granted permission for the cat necropsy and pathological examinations at Department of Pathology, Faculty of Veterinary Medicine, Selçuk University, Konya, Türkiye. During systemic necropsy, white round foci with diameters ranging from 3.00 to 5.00 mm were observed in the lung and spleen. Tissue samples were collected from the lung, spleen, liver, heart, kidney, mediastinal lymph nodes and brain for histopathological examinations. The tissues were subjected to routine histological tissue processing and sections were stained with Hematoxylin and Eosin and Ziehl-Neelsen. Additionally, Mycobacterium spp. antibodies were used for immunohistochemical staining. Microscopic examination revealed exudative tuberculosis lesions, areas of necrosis without a fibrous capsule and karyorrhectic cells only in the lung and spleen. Acid-resistant bacteria observed by ZN staining in the lesioned areas of the lung and spleen were identified as Mycobacterium spp. using immunohistochemical staining. No positive staining was observed in other organs using ZN and immunohistochemical methods. As a result, congenital tuberculosis was diagnosed in a cat for the first time, especially in relation to lesions in the spleen.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"203-205"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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