Veterinary Research Forum最新文献

{"title":"Clinical and echocardiographic evaluations of sedative and cardiovascular effects of combination of xylazine-acepromazine versus xylazine-pregabalin in horses.","authors":"Siamak Shokrollahi, Rahim Mohammadi, Farshid Sarrafzadeh-Rezaei, Ghader Jalilzadeh-Amin, Seyed Mohammad Hashemi-Asl","doi":"10.30466/vrf.2024.2013359.4034","DOIUrl":"10.30466/vrf.2024.2013359.4034","url":null,"abstract":"<p><p>The aim of this study was to compare the sedative and cardiovascular effects of the combination of xylazine-acepromazine versus xylazine-pregabalin - in horses. Four healthy crossbred horses were included in the study and assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg^-1) in combination with acepromazine maleate (0.05 mg kg^-1) intravenously. In treatment II (T2), the animals received intragastric administration of pregabalin (4.00 mg kg^-1) followed by xylazine hydrochloride (1.00 mg kg^-1) intravenously after 60 min. Head height above ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 minubefore and 5, 15, 30, 60, and 90 minu after drug administration. In T2, recordings were made 5 min before pregabalin, 55 minu after pregabalin administration, and then 5, 15, 30, 60, and 90 min after xylazine hydrochloride acepromazine injection. Analyses of the data showed there were no significant differences regarding HHAG and echocardiographic indices between the two treatments. Intragastric administration of pregabalin prior to xylazine could be considered as an alternative premedication regimen when acepromazine administration is contraindicated or undesirable.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive role of lactate in dogs with acute pancreatitis advanced to systemic inflammatory response syndrome.","authors":"Sei-Myoung Han","doi":"10.30466/vrf.2023.1990137.3795","DOIUrl":"10.30466/vrf.2023.1990137.3795","url":null,"abstract":"<p><p>Acute pancreatitis (AP) can develop into life-threatening conditions such as systemic inflammatory response syndrome (SIRS) or multiple organ dysfunction syndrome. Thirty-nine of 54 client-owned dogs admitted to the Referral Animal Medical Center and diagnosed with AP within 24 hr of onset were retrospectively reviewed to assess early predictors of progression from AP to SIRS. The patients were divided into SIRS (SIRS occurring after AP) and non-SIRS (AP occurring but no SIRS) groups. The population and mean values of laboratory variables within 24 hr of admission were assessed and compared between both groups. There were significantly more dogs with abnormal lactate levels in the SIRS group (80.00%) than non-SIRS group (11.10%). Other parameters did not differ significantly. Mean lactate level values were significantly higher at 3.64 ± 1.75 mmol in the SIRS group compared to 1.68 ± 0.52 mmol in the non-SIRS group. The increased energy required by activated immune cells may lead to metabolic changes characterized by anaerobic glycolysis and increased lactate production. This study's results suggest blood lactate monitoring in the early stages of progression from AP to SIRS in small animal clinical practice. Measuring lactate levels at the early stages of pancreatitis could lead to rapid therapeutic intervention for SIRS and ultimately reduce mortality.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of echocardiographic parameters in healthy and dilated cardiomyopathy-affected dogs.","authors":"Pritish Rath, Biswadeep Jena, Sidhartha Sankar Behera, Srinivas Sathapathy, Prasana Kumar Rath, Swetapadma Sarangi","doi":"10.30466/vrf.2023.2018338.4108","DOIUrl":"https://doi.org/10.30466/vrf.2023.2018338.4108","url":null,"abstract":"<p><p>Echocardiography is a non-invasive and gold standard imaging tool for diagnosing dilated cardiomyopathy (DCM) in dogs. This study aimed to compare the echocardiographic parameters between healthy and DCM-affected dogs. A total of 52 client-owned dogs, comprising 38 males and 14 females, were included. Among these, 24 dogs (46.15%) were classified as healthy controls and 28 dogs (53.85%) were part of DCM group. On breed-wise prevalence, it was reported that Labrador Retriever breeds showed a higher incidence of DCM than the others. The comparative studies of echocardiographic parameters showed that DCM-affected dogs had significantly higher values in left ventricular long axis length at -end diastole (LVLdA4C) and -end systole (LVLsA4C), end diastolic volume (EDV), end systolic volume (ESV), left atrium (LA)/aorta diameter (Ao) ratio, left ventricular internal dimension at systole (LVIDs), and end point septal separation (EPSS), as well as significantly lower values in left ventricular contractibility indices such as fractional shortening (FS) and ejection fraction (EF) compared to healthy dogs. Also, receiver operating characteristic curves were made to determine the optimal cut-off points for each echocardiographic parameter with specificity and sensitivity for diagnosing DCM. Significant areas under the curve were observed for parameters such as LVIDs, EF, FS, LA/Ao, EPSS, LVLdA4C, LVLsA4C, left ventricular EDV, left ventricular ESV, and ESV for DCM-affected dogs. This cut-off value can be used as an early diagnosis of DCM through echocardiography, facilitating timely clinical interventions and management strategies for improved quality of life in dogs.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active surveillance and associated epidemiological risk factors of caprine paratuberculosis in selected district of Odisha, India.","authors":"Pragnya Smita Mishra, Prasana Kumar Rath, Susen Kumar Panda, Bidyut Prava Mishra, Sangram Biswal, Smruti Ranjan Mishra, Jyotiprabha Mishra, Rajeev Ranjan","doi":"10.30466/vrf.2024.2012773.4025","DOIUrl":"https://doi.org/10.30466/vrf.2024.2012773.4025","url":null,"abstract":"<p><p>Caprine paratuberculosis (PTB) is a progressive, debilitating and production-limiting disease that causes significant economic losses and raises public health concerns. The goal was to study active surveillance and associated epidemiological risk factors of caprine PTB in selected district of Odisha, India. The 818 goats of various ages, sexes and breeds were randomly screened in ten different districts for a year based on history, clinical signs and fecal smear examination using the Ziehl-Neelsen stain, yielding an overall prevalence of 38.75%, with clinical and sub-clinical PTB at 8.06 and 30.68%, respectively. A molecular tool, IS900 polymerase chain reaction, was also used to confirm the disease. With <i>Mycobacterium avium</i> subsp<i>. paratuberculosis</i> (MAP) bacilli and endoparasite infections, the majority of affected goats (69.08%) were low shedders. Puri coastal district had the highest prevalence (52.29%) followed by Sambalpur (48.61%), while Khordha had the lowest prevalence (26.41%). Caprine PTB was more common in goats over 2 years old (51.23%), in the Ganjam breed (42.30%), in females (39.17%) and in goats housed on earthen floors (55.83%) according to chi-square analysis. The current study concluded that higher (30.68%) observations of subclinical PTB were cause of real concern due to its insidious spread as well as its zoonotic significance with potential human consequences, which requires immediate attention at all levels. Because of the public health importance of this hidden killer disease, the current findings would be useful in developing a roadmap for implementing prevention and control policies, prompting provision for adequate funding with elaborative research.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, virulence factors, and antimicrobial resistance profiles of Shiga toxin-producing <i>Escherichia coli</i> isolated from broiler chickens in Egypt.","authors":"Ibtisam Faeq Hasona,&nbsp;Salwa Mahmoud Helmy,&nbsp;Adel Mohammad El Gamal","doi":"10.30466/vrf.2021.539418.3233","DOIUrl":"https://doi.org/10.30466/vrf.2021.539418.3233","url":null,"abstract":"<p><p>Pathogenic <i>Escherichia coli</i> is one of the world's most important zoonotic foodborne pathogens and poses a serious threat to public health. We examined the prevalence, virulence genes, and antibiotic resistance profile of Shiga toxin (Stx)-producing <i>E. coli</i> (STEC) isolated from broiler chickens in the Kafr El-Sheikh governorate, Egypt. A total of 410 samples (230 cloacal swabs, 180 internal organs) were collected to isolate <i>E. coli</i>. A total of 29 (7.07%) <i>E. coli</i> isolates were recovered and identified, and 18 of them harbored <i>Stx</i> genes (<i>stx</i>). Out of 18 isolates, five (17.24%) carried the <i>stx</i> <i>1</i> gene, five (17.24%) carried the <i>stx</i> <i>2</i> gene, four (13.79%) carried both <i>stx</i> <i>1</i> and <i>stx</i> <i>2</i> genes, and four (13.79%) carried <i>stx</i> <i>1</i> <i>, stx</i> <i>2</i> <i>,</i> and <i>eae</i> <i>A</i> genes. Overall, complete anti-biotic resistance was observed against amoxicillin, ampicillin, cefpodoxime, and cefoperazone; high resistance was observed against ampicillin/sulbactam, nalidixic acid, cefuroxime, aztreonam, ciprofloxacin, ceftriaxone, chloramphenicol, sulfamethoxazole/trimethoprim, and ceftazidime; moderate resistance against gentamicin; low resistance against cefoxitin; lower resistance was detected against norfloxacin, cefotetan, and amikacin; and the lowest resistance against imipenem. All <i>E. coli</i> isolates demonstrated multidrug resistance against at least four antibiotic classes. Out of 29 <i>E. coli</i> isolates, STEC accounted for 18 isolates, of which the O78, O26:H11, O128:H2, O1:H7, O119:H6, and O91:H21 serogroups were predominant. All <i>E. coli</i> isolates were multidrug resistant and therefore pose a potential public health concern as these virulent, resistant strains may spread to humans. Thus, high levels of hygiene and biosecurity are required by chicken handlers to decrease the danger of infection spreading to humans.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1c/6d/vrf-14-131.PMC10073808.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptotic activity of Newcastle disease virus in comparison with nisin A in MDA-MB-231 cell line.","authors":"Gholam Abbas Ghorbankhani,&nbsp;Ali Mohammadi,&nbsp;Nasrin Kazemipur,&nbsp;Solmaz Morovati,&nbsp;Behrouz Gharesi Fard,&nbsp;Saeed Nazifi Habibabadi,&nbsp;Mahdi Hashempour Sadeghian","doi":"10.30466/vrf.2022.542258.3297","DOIUrl":"https://doi.org/10.30466/vrf.2022.542258.3297","url":null,"abstract":"<p><p>Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment seems essential. In this study, we evaluated the anti-tumor effects of nisin A and Newcastle disease virus (NDV) on triple-negative MDA-MB-231 cell line. The MDA-MB-231 cell line was separately and in combination subjected to the different concentrations of a Vero-adapted NDV (JF820294.1) and nisin A. The oncolytic effects of these treatments were analyzed by different cytotoxic and apoptosis techniques including trypan blue staining, MTT assay, acridine orange (EB/AO) staining, colony assay and flow cytometry over time. Nisin A at doses of more than 20.00 μg mL^-1 could represent the anti-viral effects and interfere with the oncolytic activity of NDV. Moreover, the analyses indicated that the anti-proliferative and cytotoxic features of combination therapy were stronger than those of individual NDV groups. However, the most apoptotic effect was seen in NDV experimental groups. Taken together, the results from cytotoxicity tests, flow cytometry and colony assay showed that either of the oncolytic agents had significant effects at low concentrations 72 hr post-treatment. Thereby, they had the potential to be used as new approaches in cancer treatment.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/9e/vrf-14-29.PMC9906615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of double intravenous furosemide administration at different doses on clinical variables, electrocardiographic indices and serum electrolytes in miniature donkeys.","authors":"Seyed Hesamaddin Banihashemi,&nbsp;Amir Saeed Samimi,&nbsp;Kamyab Samimi,&nbsp;Javad Tajik","doi":"10.30466/vrf.2020.127810.2954","DOIUrl":"https://doi.org/10.30466/vrf.2020.127810.2954","url":null,"abstract":"<p><p>The intravenous (IV) usage of diuretics such as furosemide may cause changes in clinical variables, electrocardiographic (ECG) indices and some serum electrolytes concentrations in miniature donkeys. The purpose of this study was to evaluate the effects of double intravenous (IV) administration of furosemide at different doses on the clinical variables, ECG indices and serum electrolytes levels in miniature donkeys. A total of 21 clinically and para-clinically healthy miniature donkeys with a mean age of 6.00 ± 1.00 years and weight of 95.00 ± 5.00 kg were used. The animals were randomly divided into three groups of control (normal saline) and low- (1.00 mg kg^-1) and high-dose (2.00 mg kg^-1) of furosemide. Two IV injections with 12-hr interval were administered in each animal. The clinical and ECG parameters were measured at 0 (baseline), 2 (T2), 24 (T24) and 48 (T48) hr after drug administration. Serum concentrations of electrolytes were measured at T0, T24 and T48. The results of this study showed no changes in clinical variables (heart rate, rectal temperature and respiratory rate) and ECG indices (amplitude and duration of P, QRS and T waves and duration of PR, QT and RR intervals) as well as no significant changes in serum electrolytes (sodium, potassium, chloride, phosphorus, calcium and magnesium) levels. Although no significant effect on clinical variables, electrolytes levels and ECG indices after double IV administration of furosemide (1.00 and 2.00 mg kg^-1) in miniature donkeys was observed, absence of these effects does not mean that furosemide had no pharmacological effect.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/a7/vrf-14-53.PMC9906614.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights on skewing of sex ratio in rabbits (<i>Oryctolagus cuniculus</i>) supplemented with dietary calcium and magnesium.","authors":"Sharanya Jeevendra Naidu,&nbsp;Arangasamy Arunachalam,&nbsp;Akeem Babatunde Sikiru,&nbsp;Selvaraju Sellappan,&nbsp;Backialakshmi Sekar,&nbsp;Ippala Janardhan Reddy,&nbsp;Raghavendra Bhatta","doi":"10.30466/vrf.2022.551122.3428","DOIUrl":"https://doi.org/10.30466/vrf.2022.551122.3428","url":null,"abstract":"<p><p>The effect of dietary calcium (Ca) and magnesium (Mg) supplementation on serum biochemical parameters, steroid hormones, gene expression, and the sex ratio was investigated in female New Zealand white rabbits. A total of 25 rabbits were allocated into five treatment groups: The control group was fed with regular pellet feed, whereas, treatment groups were supplemented with Ca and Mg: T1 (0.40% and 0.01%), T2 (0.60% and 0.02%), T3 (0.80% and 0.03%) and T4 (1.00% and 0.04%), respectively. The rabbits were subjected to three breeding cycles. The T3 group skewed towards females (65.33%) from all three breeding. There was elevated Ca concentration in T3 (15.26 ± 0.77 mg dL^-1) and T4 (15.61 ± 0.82 mg dL^-1) groups compared to the control. The concentration of estradiol was significantly high in T3 and T4 groups at 0.5 days post-coitus (dpc) and T2, T3 and T4 groups at 21dpc. Testosterone was significantly high in T4 group at 0.50 dpc and T2 and T4 group at 21dpc. The expression of 13 genes was studied in the oviduct. Genes such as <i>OVGP1, CCT4, ANXA2</i> and <i>TLR4</i> were up-regulated and positively correlated with the female sex ratio. The molecular functions and pathways of up-regulated genes were suggestive of their role in fertilization such as sperm selection, sperm storage, immune regulation, implantation and early embryonic development. The variations in the serum electrolytes, steroid hormones and gene expression might have an impact on the skewing process.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/94/d2/vrf-14-405.PMC10475169.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10217299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livestock, pets and humans as carriers of methicillin-resistant <i>Staphylococcus</i><i>aureus</i> and comparative evaluation of two PCR protocols for detection.","authors":"Dharitree Sonowal,&nbsp;Sandeep Ghatak,&nbsp;Acheenta Gohain Barua,&nbsp;Srinivas Kandhan,&nbsp;Razibuddin Ahmed Hazarika,&nbsp;Arnab Sen,&nbsp;Samir Das,&nbsp;Sarat Sonowal,&nbsp;Rajeev Kumar Sharma,&nbsp;Shantanu Tamuly,&nbsp;Chimanjita Phukan,&nbsp;Ajanta Sharma,&nbsp;Poznur Hussain","doi":"10.30466/vrf.2023.1971891.3672","DOIUrl":"https://doi.org/10.30466/vrf.2023.1971891.3672","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> are Gram positive bacteria known to acquire antibiotic resistance rapidly and pose a major challenge to clinicians worldwide. Infections by methicillin resistant <i>Staphylococcus aureus</i> (MRSA) are usually associated with increased mortality and prolonging of treatment. Samples (n = 706) from diverse sources (livestock, pets, animal handlers, human hospital) were collected and screened for the presence of MRSA by phenotypic and genotypic methods. The incidence of <i>Staphylococcus aureus</i> was greater in goats (42.00%; 28.20 - 56.80%, confidence interval [CI] 95.00%) followed by cattle (13.50%; 9.20 - 18.80%, CI 95.00%), humans (12.90%; 9.30 - 17.40%, CI 95.00%) and dogs (12.90%; 8.10 - 19.20%, CI 95.00%). Significantly higher incidence of MRSA was observed in dogs (65.00%; 40.80 - 84.60%, CI 95.00%), compared to other hosts namely cattle (48.00%; 26.50 - 64.30%, CI 95.00%), humans (35.00%; 20.20 - 52.50%, CI 95.00%) and goats (10.00%; 1.20 - 30.40%, CI 95.00%). All the <i>S. aureus</i> isolates were further screened for thermostable nuclease (<i>nuc</i> gene) by polymerase chain reaction (PCR). The incidence of <i>nuc</i> gene in cattle, dog, goat and human were found to be 3.30% (1.30 - 6.60%, CI 95.00%), 5.20% (2.30 - 9.90%, CI 95.00%), 28.00% (16.20 - 42.50%, CI 95.00%) and 9.10% (6.00 - 13.00%, CI 95.00%), respectively. Comparative evaluation of two PCR primers (<i>mecA</i>-162 and <i>mecA</i>-310) indicated the former one as more rational choice for detection of MRSA. Overall, the results of our study indicated possible risk of zoonotic transmission of MRSA from canines.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/04/vrf-14-351.PMC10410111.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progesterone circulation and follicular growth in adapted <i>Bos taurus</i> cows.","authors":"Héctor Javier Narváez,&nbsp;Luis Ernesto Quintero Rodríguez","doi":"10.30466/vrf.2022.554987.3489","DOIUrl":"https://doi.org/10.30466/vrf.2022.554987.3489","url":null,"abstract":"<p><p>The creole breed Blanco Orejinegro (<i>Bos taurus</i> adapted) is an important animal genetic resource in Colombia. However, very little is known about the reproductive physiology of the breed. The objective was to determine the dynamics of progesterone circulation and follicular growth in Blanco Orejinegro cows throughout the estrous cycle. Fifteen cyclic Blanco Orejinegro cows were used and subjected to hormonal protocol for estrous and ovulation synchronization. Once the time of ovulation was identified by monitoring the ovarian dynamics with ultrasonography equipment. For description of the results, the mean was used as a measure of central tendency and the standard error of the mean. Statistical analysis was longitudinal descriptive. Blood samples were collected every 24 hr throughout the estrous cycle for serum quantification of progesterone (P4), monitoring of corpus luteum (CL) growth dynamics and follicular dynamics. It was possible to verify that 76.92% of the Blanco Orejinegro cows presented two follicular waves during the estrous cycle and the highest circulating levels of P4 (> 6.00 ng mL^-1) were observed on the 14^th day of the estrous cycle. It was concluded that the creole cows of the Blanco Orejinegro breed presented two follicular waves per estrous cycle. In addition to, the size of the CL was consistent with the secretion of progesterone presenting higher circulating levels at the end of the luteal phase.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/e2/vrf-14-397.PMC10410107.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}