Veterinary Research Forum最新文献_第7页

Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report. 使用卡泊芬净成功治疗白色念珠菌诱发的犬真菌性膀胱炎：病例报告。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2018960.4113

Sei-Myoung Han

{"title":"Successful treatment of Candida albicans-induced fungal cystitis in a dog using caspofungin: a case report.","authors":"Sei-Myoung Han","doi":"10.30466/vrf.2024.2018960.4113","DOIUrl":"https://doi.org/10.30466/vrf.2024.2018960.4113","url":null,"abstract":"A 7-year-old castrated male poodle was brought to the referral Animal Medical Center and diagnosed with diabetes and pancreatitis. One month later, the patient presented with cloudy urine, and ultrasonography revealed a large number of spherical substances. The patient was subsequently diagnosed with fungal cystitis with Candida albicans. Initially, 10.00 mg kg-1 itraconazole was prescribed twice daily for six weeks, and the symptoms of prolonged urination improved; however, the fungal balls persisted in the bladder. The six months later, the patient showed recurrent symptoms, such as dysuria and stranguria; therefore, 5.00 mg kg-1 fluconazole was prescribed twice daily; however, it was not effective. Subsequently, 1.00 mg kg-1 caspofungin once daily was administered for three consecutive days. Finally, the fungal balls in the bladder disappeared. The patient was regularly monitored after completion of treatment and, 17 months later, doing well without recurrence. Few reports exist on the use of caspofungin in veterinary medicine. The recommended dose of caspofungin in dogs remains unknown. In the case of azole-resistant Candida, treatment using caspofungin should be considered; although, additional studies on the established dosing and side effects are needed.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"435-438"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial. 在传统牧场系统中对奶牛进行分娩和分娩管理培训的效果：现场试验。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2007348.3945

Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori

{"title":"Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: a field trial.","authors":"Hadi Keshipour, Alireza Bahonar, Mehdi Vodjgani, Ehsan Anassori","doi":"10.30466/vrf.2023.2007348.3945","DOIUrl":"10.30466/vrf.2023.2007348.3945","url":null,"abstract":"The interval between parturition and subsequent pregnancy is called the days open or calving to conception interval and is affected by several factors, especially dystocia. Dystocia is an ab-normal or difficult calving that may require assistance during labor. This study is a field trial in health education and the research team developed a comprehensive training program for farmers to educate them about the normal process of parturition in dairy cows and when and how to assist in parturition or dystocia. A series of classes was held for farmers and the study covered 486 multi-parous dairy cows, with 173 belonging to the group of trained farms (educated farmers) and 313 to the control group (non-educated farmers). Although dystocia was lower in the educated group, there were no significant differences in retained placenta between two groups. However, cows in the educated group had a better conception rate (lower service per conception) in sub-sequent parturitions. Hence, the median number of days open for cows from trained farmers was significantly lower than other farmers (85 days compared to 120 days, respectively). Based on Cox regression analysis, uterine prolapse, retained placenta, and dystocia could significantly impact subsequent pregnancies. Dystocia affects days open, and training on parturition and dystocia management can effectively reduce the numbers of days open in dairy cows.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"139-144"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of biofilm forming fungal species and in vitro evaluation of anti-biofilm activity of disinfectants used in drinking water. 生物膜形成真菌的发生及饮用水消毒剂抗生物膜活性的体外评价。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2016504.4088

Juon Abbass, Muhammad Ashraf, Serpil Kahya Demirbilek, Merve Yıldız, Havva Aner, Ali Raza, Kamil Tayfun Carlı

{"title":"Occurrence of biofilm forming fungal species and in vitro evaluation of anti-biofilm activity of disinfectants used in drinking water.","authors":"Juon Abbass, Muhammad Ashraf, Serpil Kahya Demirbilek, Merve Yıldız, Havva Aner, Ali Raza, Kamil Tayfun Carlı","doi":"10.30466/vrf.2024.2016504.4088","DOIUrl":"10.30466/vrf.2024.2016504.4088","url":null,"abstract":"Fungal contamination in drinking water has garnered considerable attention over the past few decades, especially considering the detrimental consequences of pathogenic fungal species on both human and animal health. The formation of biofilms by certain species is a considerable factor contributing to the emergence of severe fungal infections. This research was designed to isolate and identify fungi, particularly those capable of forming biofilms from 150 samples of drinking water sourced from various locations. The isolated fungal species were tested for them in vitro biofilm formation using a microtitration plate method and the crystal violet assay was applied to quantify the established biofilms. The effectiveness of three disinfectants, namely ozone, chlorine, and hydrogen peroxide, in preventing the formation of biofilms by the most isolated fungal species was monitored. The findings indicated that Aspergillus species were the most prevalent in drinking water, comprising 63.33% (95/150) of the total number of fungal species identified. Aspergillus fumigatus and Aspergillus flavus were identified as the primary contributors to biofilm formation in drinking water distribution systems with prevalence rates of 41.00 and 34.00%, respectively, among all Aspergillus species. The outcomes of the in vitro studies demonstrated that the ozone disinfectant exhibited promising results in inhibiting fungal biofilms compared to chlorine and hydrogen peroxide. In conclusion, these findings provided valuable insights for water distribution authorities to develop effective regimens for controlling biofilm-forming fungal species using suitable antifungal biofilm disinfectants.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"651-656"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of melatonin on the mitogen-activated protein kinase signaling genes in hypoxic Leydig cells. 褪黑素对缺氧间质细胞中丝裂原活化蛋白激酶信号基因的影响。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-12-15 DOI: 10.30466/vrf.2024.2026202.4231

Mona Ghasemi, Adel Saberivand, Mohammadreza Gholami, Vahideh Assadollahi, Masoud Alasvand

{"title":"Effects of melatonin on the mitogen-activated protein kinase signaling genes in hypoxic Leydig cells.","authors":"Mona Ghasemi, Adel Saberivand, Mohammadreza Gholami, Vahideh Assadollahi, Masoud Alasvand","doi":"10.30466/vrf.2024.2026202.4231","DOIUrl":"10.30466/vrf.2024.2026202.4231","url":null,"abstract":"Leydig cells play a crucial role in male reproductive physiology, and their dysfunction is often associated with male infertility. Hypoxia negatively affects the structure and function of Leydig cells. This study aimed to investigate the impact of melatonin on the c-Jun N-terminal kinase (Jnk), P38, and extra-cellular signal-regulated kinases 1 and 2 (Erk1/2) mitogen-activated protein kinase (MAPK) signaling pathways in TM3 mouse Leydig cells under hypoxia induced by cobalt (II) chloride (CoCl2). The TM3 cell line was utilized as a subject of research, and 100 μM CoCl2 was employed to induce hypoxia. Following the addition of 10.00 ng mL-1 melatonin, quantitative reverse transcription-polymerase chain reaction and western blot analyses were conducted to assess the gene expression and protein level of Jnk, p38, and Erk1/2, while enzyme-linked immunosorbent assay was used to measure testosterone secretion. The results showed that melatonin significantly increased testosterone production in the CoCl2 + melatonin group compared to the CoCl2-treated group. Furthermore, melatonin elevated both the protein level and mRNA expression of Erk1/2, Jnk, and p38 genes in the CoCl2 + melatonin group compared to the CoCl2 group. In conclusion, melatonin activated the Jnk, p38, and Erk1/2 MAPK signaling pathways and enhanced testosterone production in the presence of CoCl2 in TM3 cells.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"673-680"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and implementation of a TaqMan^® real-time PCR method for detection and quantification of bovine leukemia virus. 设计并实施用于检测和定量牛白血病病毒的 TaqMan® 实时 PCR 方法。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.30466/vrf.2024.2016741.4084

Hassan Vahidi Emami, Arash Ghalyanchi Langeroudi, Seyed Masoud Hosseini, Hamideh Najafi

{"title":"Design and implementation of a TaqMan® real-time PCR method for detection and quantification of bovine leukemia virus.","authors":"Hassan Vahidi Emami, Arash Ghalyanchi Langeroudi, Seyed Masoud Hosseini, Hamideh Najafi","doi":"10.30466/vrf.2024.2016741.4084","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016741.4084","url":null,"abstract":"The bovine leukemia virus (BLV) is an important infectious agent transmitted from cattle to humans. It is considered one of the oncogenic viruses in breast cancer, so an accurate detection of this virus is important. The study aimed to design a specific and sensitive method based on TaqMan® real-time polymerase chain reaction (RT-PCR) for BLV detection. Probes and primers were designed using bioinformatics software for a 108 pairs region of the BLV tax gene. Criteria employed for determining analytical sensitivity were prepared using in-vitro RNA transcriptions. The National Center for Biotechnology Information (NCBI), basic local alignment search tool (BLAST) databases various viral panels and genomic samples from healthy individuals (Qom Province, Iran in 2023) were used to verify analytical specificity and clinical specificity, respectively. This method can measure a minimum of 10 copies of DNA and RNA mL-1. Moreover, the assay is linear in the range of 100 - 109 copies mL-1. By testing negative specimens, the method specificity was 100%. The reproducibility results of the reaction were examined at the intra- and inter-assay comparison. In fact, 10 technical replicates of each concentration of the control sample were analyzed in each working reaction. Due to the locally made kit, exact sensitivity and specificity, rapid analysis, and relatively low cost, as compared to commercial kits of other countries, the method introduced in the present study could be suitable for accurate detection of the BLV. Also, the TaqMan® real-time PCR method could be detected in cattle and human and before malignant changes of breast cancer which could reduce infection and breast cancer.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"417-423"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal protection by ellagic acid in a rat model of glycerol-induced acute kidney injury. 鞣花酸在甘油诱发急性肾损伤大鼠模型中对肾脏的保护作用

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.2000658.3859

Maryam Khombi Shooshtari, Alireza Sarkaki, Mohammad Rashno, Khojasteh Hoseinynejad

{"title":"Renal protection by ellagic acid in a rat model of glycerol-induced acute kidney injury.","authors":"Maryam Khombi Shooshtari, Alireza Sarkaki, Mohammad Rashno, Khojasteh Hoseinynejad","doi":"10.30466/vrf.2023.2000658.3859","DOIUrl":"10.30466/vrf.2023.2000658.3859","url":null,"abstract":"Studies conducted on animal models have shown that the administration of glycerol can lead to kidney tissue damage and impaired renal function. This is believed to be caused by oxidative stress and inflammation, which in turn can result in elevated levels of blood urea nitrogen (BUN) and creatinine. These metabolites are commonly used as indicators of renal function. The aim of the current experimental research was to investigate the protective efficacy of ellagic acid in a rat model of rhabdomyolysis induced by glycerol. Sixty healthy adult male Wistar rats weighing between 250 - 300 g were divided into five equal groups including control, rhabdomyolysis (administered 8.00 mL kg-1 of glycerol), and three rhabdomyolysis plus various doses of ellagic acid (25.00, 50.00 and 100 mg kg-1 per day; 72 hr after receiving glycerol for 14 days successively) groups. Serum levels of BUN, creatinine, lactate dehydrogenase, alkaline phosphatase, electrolytes and inflammatory cytokines were evaluated in all rats. Histopathological studies were also performed on kidney tissues from all groups. The administration of ellagic acid resulted in a significant increase in renal function biomarkers compared to the rats with acute kidney injury. This increase was consistent with notable reductions in tumor necrosis factor-α levels and increases in interleukin-10 levels observed in blood samples. Furthermore, the improvement in histopathological indices observed in rats received ellagic acid confirmed its nephroprotective role. The results of the current experimental study suggest that ellagic acid can improve kidney damage following glycerol injection, potentially by modulating the inflammatory process.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"105-111"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence spectroscopy and molecular modeling studies on the interaction of aflatoxin B1 and G1 with bovine α-lactalbumin. 黄曲霉毒素 B1 和 G1 与牛α-乳白蛋白相互作用的荧光光谱学和分子模型研究。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI: 10.30466/vrf.2024.2006492.3959

Omid Soltanabadi, Maliheh Sadat Atri, Mohammad Bagheri

{"title":"Fluorescence spectroscopy and molecular modeling studies on the interaction of aflatoxin B1 and G1 with bovine α-lactalbumin.","authors":"Omid Soltanabadi, Maliheh Sadat Atri, Mohammad Bagheri","doi":"10.30466/vrf.2024.2006492.3959","DOIUrl":"10.30466/vrf.2024.2006492.3959","url":null,"abstract":"Aflatoxins are toxic chemicals produced by Aspergillus fungi. Reports exist on the relationship of aflatoxin exposure via contaminated food and feed to hepatotoxicity and liver cancer. Aflatoxin B1 (AFB1) and Aflatoxin G1 (AFG1) are two dangerous types of aflatoxins for human health. Bovine α-lactalbumin (ALA) is the second major whey protein in milk which bear diverse biological functions. In this study, the interaction of AFB1 and AFG1 with the ALA protein was studied using fluorescence spectroscopy, molecular docking and molecular dynamic (MD) simulation. The spectroscopy experiments showed that the interaction with AFB1 and AFG1significantly quenched the intrinsic fluorescence emission of ALA via a static quenching mechanism. The free energy of binding and binding constant (Ka) obtained from the intrinsic fluorescence results were -5.32 kcal per mol and 0.80 × 104 L mol-1 for AFB1 and -5.64 kcal per mol and 1.35 × 104 l mol-1 for AFG1, respectively. Molecular docking studies were conducted before and after the MD simulation to estimate the binding sites, Ka s and binding mode. Results from the molecular docking showed that AFB1 and AFG1 bound to ALA via hydrophobic interaction and hydrogen bond. After MD simulation, the precision of the Ka obtained from the docking results was improved and it was more similar to the experimental results of fluorescence spectroscopy. Other simulation results were aligned well with the molecular docking and fluorescence spectroscopy results. Accordingly, AFB1and AFG1 could form complex with ALA, however, AFG1 showed higher affinity for binding to ALA and more compact complex structure.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"551-558"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Susceptibility of Pasteurella multocida isolated from cattle in Egypt to antibiotics, silver, chitosan and curcumin nanoparticles. 埃及牛群中分离出的多杀性巴氏杆菌对抗生素、银、壳聚糖和姜黄素纳米颗粒的敏感性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2017140.4090

Amany Dieb Bahr, Emil Saad Abdel Massieh

{"title":"Susceptibility of Pasteurella multocida isolated from cattle in Egypt to antibiotics, silver, chitosan and curcumin nanoparticles.","authors":"Amany Dieb Bahr, Emil Saad Abdel Massieh","doi":"10.30466/vrf.2024.2017140.4090","DOIUrl":"10.30466/vrf.2024.2017140.4090","url":null,"abstract":"Pasteurella multocida is a Gram-negative bacterium causing economically significant diseases in cattle. This study aimed to determine P. multocida susceptibility to different antibiotics and antibiotic alternatives. In this study, 246 samples (180 nasal swabs and 66 lung tissue specimens) were collected from cattle showing respiratory manifestations in Egypt. Suspected P. multocida colonies following culture were subjected to polymerase chain reaction (PCR) for molecular confirmation of the isolates. A multiplex PCR was employed to identify P. multocida capsular groups. Susceptibility of the isolated P. multocida to different antibiotics and nanoparticles as antibiotic alternatives including silver (AgNPs), chitosan (CNPs) and curcumin (CurNPs) were tested using broth microdilution method. Thirty-two P. multocida isolates were obtained, kmt1 gene was detected in these isolates, and molecular capsular types classification revealed that all isolates were belonged to the capsular type A. Based on broth microdilution method findings, 20 (62.50%) isolates were considered as multi-drug resistant (MDR); the isolates were most sensitive to danofloxacin and kanamycin, whereas they were most resistant to doxycycline and tilmicosin. Antibiotic alternatives showed high anti-microbial activity against tested isolates with minimum inhibitory concentrations ranging from 1.56 - 6.25 μg mL-1, 156 - 625 μg mL-1, and 128 - 512 μg mL-1 for AgNPs, CNPs and CurNPs, respectively. Our finding demonstrated that MDR P. multocida was evident in cattle in Egypt. Although antibiotic alternatives showed promising in vitro anti-microbial effects against MDR isolates, additional studies are required to be actually applicable in veterinary practices.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"455-462"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran. 筛查伊朗西北部牛、绵羊和山羊中分离出的耐甲氧西林金黄色葡萄球菌的万古霉素抗性相关基因。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI: 10.30466/vrf.2023.2003964.3902

Leila Maleki, Amir Tukmechi

{"title":"Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran.","authors":"Leila Maleki, Amir Tukmechi","doi":"10.30466/vrf.2023.2003964.3902","DOIUrl":"10.30466/vrf.2023.2003964.3902","url":null,"abstract":"Staphylococcus aureus is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of S. aureus. The MecA gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely vanA and vanB, were searched in the isolates. Out of 400 nasal swabs, 69 samples had S. aureus; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 3","pages":"159-164"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages. 不同胚胎和胎儿阶段鸵鸟（Struthio camelus）气管和肺的形态发生。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2023-06-15 DOI: 10.30466/vrf.2023.2009800.3984

Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar

{"title":"Morphogenesis of the ostrich (Struthio camelus) trachea and lung in different embryonic and fetal stages.","authors":"Ahmadreza Raji, Shadi Hashemnia, Ahmad Ali Mohammadpoor, Abolghasem Nabipour, Zohre Saadatfar","doi":"10.30466/vrf.2023.2009800.3984","DOIUrl":"10.30466/vrf.2023.2009800.3984","url":null,"abstract":"The ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock industry. This research was conducted to analyze the development of the ostrich respiratory system during fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms and then incubated at 36.00 - 37.00 ˚C and 25.00 ± 2.00% humidity for 40 days. Sections were taken on days 13, 22, 26, 30, 36, and 42 of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by Hematoxylin and Eosin, Alcian Blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques. It was concluded that the trachea in the 13-day-old embryo and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilage and differentiation of mesenchymal to the loose connective tissue. The bronchial stage of the lung was observed in the 22-day-old embryo, pseudoglandular stage in the 26-day-old embryo, and parabrachial and air capillary stage in the 30-day-old embryo. The information obtained from this study will be useful for diagnosing pathologies affecting this vital system and results in improving industrial breeding management.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"297-301"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0