Veterinary Research Forum最新文献

{"title":"An analysis of oxidative stress indices and clinical parameters in budgerigars (<i>Melopsittacus undulatus</i>) treated with medetomidine-ketamine and midazolam-ketamine.","authors":"Zahra Amini, Fatemeh Hoseinpour, Ali Ghashghaii, Hadi Cheraghi","doi":"10.30466/vrf.2023.1999287.3836","DOIUrl":"10.30466/vrf.2023.1999287.3836","url":null,"abstract":"<p><p>Various companion birds, including budgerigars, are anesthetized with injectable anesthesia. The current study aimed to evaluate oxidative stress indices including malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) along with clinical parameters such as the time required to induce, maintain and recover from medetomidine-ketamine anesthesia and midazolam-ketamine anesthesia in budgerigars. Among 20 mature and healthy budgerigars, three groups were assigned as follows: Control (n = 4) to determine baseline oxidative stress indices medetomidine + ketamine (n = 8) anesthetized by intramuscular injections of medetomidine (0.04 mg kg^-1) and ketamine (30.00 mg kg^-1) in the pectoral muscles, midazolam + ketamine (n = 8) anesthetized by intramuscular injections of midazolam (1.00 mg kg^-1) and ketamine (50.00 mg kg^-1). Half of birds (n = 4) in the second and third groups were euthanized by cervical dislocation 1 hr after anesthesia induction, blood samples were collected directly from the heart, and sera were extracted. Additionally, the remaining birds were euthanized 24 hr later, and their serum was analyzed for oxidative stress indices. Clinical parameters were recorded during the study. Compared to the medetomidine + ketamine group, the midazolam + ketamine group experienced shorter induction, anesthetic, and recovery times. Administering medetomidine and ketamine elevated TOS levels compared with midazolam + ketamine. No significant difference was found between the test groups for TAC, MDA, or OSI. Therefore, the midazolam + ketamine regimen appears superior to medetomidine + ketamine when performing minor surgeries on budgerigars.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"237-242"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of osteogenesis and antibacterial properties of a bioactive glass/gelatin-based scaffold using zoledronic acid and CM11 peptide.","authors":"Soheil Vazifehdoust, Ali Shalizar-Jalali, Mohammad Reza Nourani, Mehrdad Moosazadeh Moghaddam, Mohsen Yazdanian","doi":"10.30466/vrf.2024.2020333.4136","DOIUrl":"10.30466/vrf.2024.2020333.4136","url":null,"abstract":"<p><p>This study aimed to investigate the effects of zoledronic acid (ZA) and antibacterial CM11 peptide on the osteoinduction and antibacterial properties of bioactive glass (BG). The bioactive glass/gelatin (BG/Gel) composite was synthesized using the sol-gel method. The 2-x minimum inhibitory concentration of the peptide and 4.00 mg mL^-1 of ZA were added to the BG/Gel during fabrication. The BG/Gel composite morphological and structural characteristics and anti-bacterial activities were analyzed using Fourier transform infra-red spectroscopy, scanning electron microscopy and disk diffusion test, respectively. The release of the peptide and ZA from BG/Gel was measured using ultra-violet spectroscopy. After 14 days, the effects of the peptide/ ZA-containing BG/Gel (PZ-BG/Gel) on the growth and differentiation of mesenchymal stem cells were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, calcium and alkaline phosphatase assays, immunocytochemical staining for osteocalcin (OCN) and real-time polymerase chain reaction for <i>OCN</i>, <i>type I collagen</i>, <i>bone morphogenetic protein 2</i> and <i>Runt-related transcription factor-2</i> genes. The disk diffusion test showed the anti-microbial activity of the scaffold against multi-drug-resistant isolates of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. Analyses showed a significantly higher level of stem cells differentiation into the osteo-genic cells in PZ-BG/Gel scaffold compared to BG/Gel scaffold alone. Accordingly, osteoblast markers were significantly increased in comparison with the control. In conclusion, the osteo-induction and antibacterial properties of BG-based scaffold can be improved using ZA and CM11.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"487-498"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin alleviates inflammatory effects of ketamine anesthesia in postnatal rats.","authors":"Soroush Afshar Ghahremani, Abbas Raisi, Sohrab Minaei Beirami, Houman Kahroba, Mahnaz Mardani, Omid Dezfoulian, Vahideh Tarhriz","doi":"10.30466/vrf.2024.2018359.4107","DOIUrl":"10.30466/vrf.2024.2018359.4107","url":null,"abstract":"<p><p>Curcumin has been employed in traditional medicine for over a millennium to treat various ailments, and its global use is now widespread. Chinese medicine relies heavily on curcumin as a primary element and uses it to cure infectious diseases, skin disorders, depression, and stress. It has cardioprotective, neuroprotective, and anti-diabetic properties, as well as pharmacological effects on disorders like type II diabetes, atherosclerosis, and human immunodeficiency virus replication. The anti-cancer activity of curcumin has been studied extensively with notable improvements in gastrointestinal, melanoma, urogenital, breast, and lung malignancies. We investigated the anti-inflammatory effects of curcumin on expression of <i>tumor necrosis factor</i> (<i>TNF</i>)<i>-α</i>, <i>c-Fos</i>, and <i>interleukin</i> (<i>IL</i>)<i>-6</i> genes in brain and liver tissue owing to the effects of ketamine anesthesia on postnatal rats. The thalamic and hepatic tissues were collected without anesthesia, immediately after anesthesia, and 4 and 12 hr after anesthesia in control and curcumin treated postnatal rats. The results showed that glucose, triglyceride, high- and low-density lipoprotein levels were lowered with curcumin treatment. We also found that ketamine increased <i>c</i>-<i>Fos</i> and inflammatory cytokines like <i>TNF-α</i> and <i>IL-6</i>, all of which contribute to inflammation. Brain and liver immunohistochemistry studies confirmed the real-time polymerase chain reaction findings. Curcumin injections alone may be effective in decreasing ketamine-induced inflammation in both brain and liver tissues.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"473-480"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin-3-gallate affects the quality of fresh and frozen-thawed semen of Simmental bull by two different cryopreservation methods.","authors":"Abolfazl Parvizi Alan, Esmail Ayen, Amir Khaki, Ali Soleimanzadeh","doi":"10.30466/vrf.2024.2000236.3852","DOIUrl":"https://doi.org/10.30466/vrf.2024.2000236.3852","url":null,"abstract":"<p><p>During the freezing process of semen, due to the generating of significant amounts of free radicals, the quality of sperm changes. Epigallocatechin-3-gallate (EGCG) is a green tea catechin, which in this study was applied to investigate its effect on the quality of bulls' sperm. We collected semen samples with an artificial vagina from 12 Simmental bulls to evaluate the effect of EGCG (10.00 and 20.00 µmol) in two cryopreserving methods on the quality parameters of semen. We designed six groups including two control groups (method one and two) and four treatments (EGCG 10.00 µmol + method one; EGCG 20.00 µmol + method one; EGCG 10.00 µmol + method two; EGCG 20.00 µmol + method two). The 20.00 µmol EGCG and a method two significantly affected the amending oxidative conditions as well as an increase in total antioxidant capacity and a decrease in malondialdehyde. The effect of EGCG in both concentrations was more on method two. The desired impact on sperm motility, viability, inhibition of lipid peroxidation and sperm DNA damage was observed in EGCG groups compared to control groups. Among the two methods, the method two had fewer adverse effects on the plasma membrane, motility parameters, viability and DNA of sperm. The EGCG in the semen extender yielded a favorable impact on thawed sperm. This effect was prompted in combination with the method two.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"369-377"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating potential of H'mong chicken bone marrow-derived mesenchymal stem cells at different ages for primordial germ cells' feeder layer.","authors":"Van Hanh Nguyen, Thi Huong-Giang Tran, Hong Thi Nguyen, Hiep Thi Nguyen, Nhung Thi Nguyen, Tien Phat Do","doi":"10.30466/vrf.2024.2016008.4070","DOIUrl":"https://doi.org/10.30466/vrf.2024.2016008.4070","url":null,"abstract":"<p><p>Primordial germ cells (PGCs) have potential applications in genetic conservation, vaccination, tissue repair therapies, and genetic research. Chicken bone marrow-derived mesenchymal stem cells (cbMSCs) is a good candidate for co-culture with PGCs. However, there is no consensus on the optimal age of donors. In this study, we aimed to compare specific parameters of H'Mong cbMSCs obtained from day 14^th and 19^th embryos, and day 3^rd newborns. Isolated cbMSCs showed characteristics of MSCs. Cells had fibroblast-like morphology, plastic-adherent, expressed specific markers of MSCs and multilineage differentiation potential. The growth rate of cells from day 19^th embryos was higher than from other ages. Moreover, cells expressed markers of pluripotency such as <i>Nanog</i>, <i>PouV</i>, <i>Sox2</i>, <i>CVH</i>, <i>DAZL</i>, and <i>KIT</i>, known for their role in maintaining stem cell self-renewal and pluripotency. As feeder cells, cbMSCs from three different ages promoted proliferation of H'Mong PGCs during co-culture. These results suggested that cbMSCs from different ages can be used for co-culture H'Mong PGCs which were further used for genetic preservation of H'Mong chicken or gene editing research.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"335-342"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and evaluation of controlled released implant containing mesoporous selenium nanoparticles loaded with curcumin in rats with spinal cord injury.","authors":"Ehsan Lajmiri, Moosa Javdani, Pegah Khosravian, Mohammad Hashemnia, Hossein Kazemi Mehrjerdi","doi":"10.30466/vrf.2024.2014162.4040","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014162.4040","url":null,"abstract":"<p><p>In this study, a controlled released delivery drug system designed and synthesized by loading curcumin and selenium nanoparticles (SeNaPs) on chitosan hydrogel, and while evaluating the physicochemical properties of the prepared drug delivery system, the tissue changes caused by the local implant of that system in rats with experimental spinal cord injury (SCI) were investigated. For this purpose, 100 adult female rats were randomly divided into five equal groups which are: Control group without any treatment for SCI, chitosan group that received chitosan hydrogel, curcumin group that received curcumin-loaded hydrogel, SeNaP group that received chitosan loaded with SeNaPs and SeNPCur group that received chitosan loaded with SeNaPs and curcumin. On the 3^rd and 7^th days of the study, severe infiltration of leukocytes, especially lymphocytes, as well as axon swelling and hemorrhagic necrosis at the lesion sites were observed in all groups, especially the control group. On the 7^th day, the severity of these injuries decreased in the SeNPCur group and the highest number of astrocytes was observed in this group. In addition, on the 14^th and 21^st days of the study, the lowest severity of nerve tissue damage and the lowest presence of inflammatory cells along with the highest number of astrocytes were seen in the SeNPCur group. The glial fibrillary acidic protein study also confirmed the presence of more and significant astrocytes in the SeNPCur, curcumin and SeNP groups at different times of the study, respectively. The histopathological results showed the neuroprotective effects of chitosan hydrogel loaded with selenium and curcumin.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"357-367"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of wet feeding on performance, blood plasma indices, intestinal histomorphometry, and economics of broiler chickens under hot climatic conditions.","authors":"Hina Ashraf Waiz, Lokesh Gautam","doi":"10.30466/vrf.2024.2019127.4115","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019127.4115","url":null,"abstract":"<p><p>The study aimed to investigate the impact of wet feeding on broiler chicken performance and intestinal histomorphometry. The research was conducted at the Veterinary College, Udaipur, India, using 144 commercial broiler chicks from day 1 to 6 weeks of age. The chicks were divided randomly into three experimental groups, each with four replicates comprising 48 birds. The control group (G1) was fed dry; while, group 2 (G2) received wet feed supplemented with 1.00 g water <i>per</i> g of feed and group 3 (G3) received wet feed supplemented with 1.20 g water <i>per</i> g of feed. Results revealed that G3 had significantly superior feed conversion ratios, lower feed intake, and increased body weight and weight gain compared to G2 and G1. Among the three treatment groups, there were no variations in blood plasma indicators. In comparison with G1, duodenum and ileum in G2 had higher villus height. The G3 revealed higher net profit margins and 18.89% saving of feed compared to G1, suggesting that it may be a valuable tool for lowering chicken feed costs.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"391-396"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of <i>Anaplasma platys</i> in dogs from the municipality of Veracruz, Mexico.","authors":"Izcalli Alejandra Jeréz-Sulvarán, David Itzcóatl Martínez-Herrera, Héctor Vivanco-Cid, José Alfredo Villagómez-Cortés, Héctor Alejandro Contreras-López, Lucy Anahi Muñoz-Muñoz, José Luis Hernández Vivanco, Argel Flores Primo","doi":"10.30466/vrf.2024.2021313.4154","DOIUrl":"10.30466/vrf.2024.2021313.4154","url":null,"abstract":"<p><p>Infectious canine cyclic thrombocytopenia or canine anaplasmosis is an infectious disease caused by <i>Anaplasma platys</i>. In Mexico, cases of human infection have been reported. The present cross-sectional study aimed to determine the frequency of <i>A. platys</i> infection in the municipality of Veracruz, Mexico, by nested polymerase chain reaction method. A total of 100 blood samples from dogs living in the municipality were collected and analyzed between March and June 2022. A descriptive analysis of blood samples for hemoparasites frequencies was performed with the free online software VassarStats. The evaluated variables were sex, street access, usage of ticks control methods, and living environment. The free online software WinEpi software was used to calculate odds ratio (OR) and confidence interval (CI: 95.00%). Out of 100 analyzed blood samples, 27 were confirmed positive for <i>A. platys</i>. The only risk factor found was the absence of tick prevention methods (OR = 9.81; 95.00% CI: 23.00 - 44.50). In conclusion, the frequency of <i>A. platys</i> was 27.00% and no risk factors were identified.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"697-700"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of gamma irradiation and disaccharide trehalose to induce immune responses in Syrian hamster model against Iranian SARS-CoV-2 virus isolate.","authors":"Farahnaz Motamedi-Sedeh, Akbar Khorasani, Mohsen Lotfi, Seyed Morteza Moosavi, Arash Arbabi, Seyedeh Maede Hosseini","doi":"10.30466/vrf.2024.2022838.4172","DOIUrl":"10.30466/vrf.2024.2022838.4172","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is the causative agent of the emerging zoonotic respiratory disease. One of the most important prerequisites for combating emerging diseases is the development of vaccines within a short period of time. In this study, antigen-irradiated, inactivated SARS-CoV-2 viruses and the disaccharide trehalose were used to enhance immune responses in the Syrian hamster. The SARS-CoV-2 virus was isolated from tracheal swabs, confirmed by real-time polymerase chain reaction, and propagated on Vero cells. For inactivation, it was irradiated with 14.00 kGy gamma radiation. Evaluation of the antigenic properties of the spike protein subunit S1 showed that the antigens were intact after gamma irradiation. The gamma-irradiated and formalin-treated viruses were used to immunize hamsters in four vaccine formulations. Neutralizing antibodies increased significantly in all vaccinated groups three weeks after the second and third vaccinations. The concentration of secretory immunoglobulin A in the irradiated vaccine plus trehalose increased significantly in nasal lavage and nasopharyngeal-associated lymphoid tissue fluids three weeks after the second and third vaccinations. The lymphocyte proliferation test in the spleen showed a significant increase in all vaccinated hamsters, but the increase was greater in irradiated vaccine plus trehalose and irradiated vaccine plus alum. We can recommend the irradiated inactivated vaccine SARS-CoV-2 plus trehalose (intra-nasal) and another irradiated inactivated vaccine SARS-CoV-2 plus alum (subcutaneous) as safe vaccines against coronavirus disease of 2019 (COVID-19), which can stimulate mucosal, humeral, and cellular immunities. However, the protectivity of the vaccine against COVID-19 in vaccinated hamsters must be investigated in a challenge test to assess the potency and efficiency of vaccine.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"681-689"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of curcumin-loaded polycaprolactone scaffold on Achilles tendon repair in rats.","authors":"Narges Sufian, Mehdi Behfar, Rahim Hobbenaghi, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2029563.4286","DOIUrl":"10.30466/vrf.2024.2029563.4286","url":null,"abstract":"<p><p>Scaffolds play a crucial role in tendon healing by providing structural support, promoting cell infiltration, and guiding tissue regeneration. Polycaprolactone (PCL) has been used as a polymer in biological scaffolds for several tissue engineering studies. This study aimed to investigate the effects of curcumin-loaded PCL scaffold on Achilles tendon using a tenotomy model in rats. Twenty adult male Wistar rats were randomized into two groups. In control group, tenotomy and suture placement were performed. The identical intervention followed by the implantation of curcumin-loaded PCL scaffold around the tendon stumps was performed in the treatment group. The nanofibrous PCL scaffold containing 5.00% curcumin was fabricated by electrospinning. Walking track analysis was performed weekly. Then, after 6 weeks, histopathological examination and tendon mechanical tests were performed. The weekly walking track analysis revealed a significant improvement in Achilles functional index in scaffold-treated rats from week three to six. The rate of functional improvement was remarkably slower in the control group. Histopathological examination revealed aseptic inflammation and enhanced neovascularization in the treatment group. Also, collagen arrangement and density were significantly improved in this group compared to the control samples including less regular orientation and loose organization of collagen fibers. Significant increase in mechanical properties, except for strain, was observed in the treatment group. The present study demonstrated that implantation of curcumin-loaded PCL scaffold resulted in increased fibrillar architecture, as well as improved mechanical properties and Achilles functional index in rats. To reduce the biodegradation-induced inflammation, an anti-inflammatory treatment is recommended.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"621-627"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}