Veterinary Research Forum最新文献

{"title":"Immuno-chromatography based rapid detection of canine distemper in dogs and its comparison with RT-PCR.","authors":"Jharna Koul, Divya Agnihotri, Ricky Jhambh, Tarun Kumar, Gaurav Charaya","doi":"10.30466/vrf.2024.2030571.4301","DOIUrl":"10.30466/vrf.2024.2030571.4301","url":null,"abstract":"<p><p>The present study was planned to confirm the clinical diagnosis of canine distemper in dogs with reverse transcriptase polymerase chain reaction (RT-PCR) and its comparison with lateral flow assay based immuno-chromatographic (IC) technique. Fifty clients owned dogs having clinical signs suggestive of respiratory, skin or nervous form of canine distemper were included in the study. An immuno-chromatography-based test was applied using serum to screen each of the suspected dog. In addition to serum, nasal discharges of 15 cases and ocular discharges of 10 samples were used to diagnose canine distemper. Screening with IC kit revealed 72.00% serum samples positive, 66.66% ocular and 50.00% nasal samples were found positive for antigen. The RT-PCR targeting N gene of canine distemper virus was used for the molecular diagnosis of canine distemper. Out of 50 blood samples tested with RT-PCR assay, 38 (76.00%) samples were positive showing characteristics band of 287bp. Statistical comparison of IC Kit (serum samples) results with RT-PCR results comparison showed that IC kit was 52.00% accurate with 36.84% sensitivity, 100% specificity, positive predictive value of 93.33% and negative predictive value of 31.43%. In the current study it was observed that the IC test was rapid, quick and specific but was found to be less sensitive compared to RT-PCR.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 5","pages":"261-265"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A reverse transcription recombinase-aided amplification assay for the rapid detection of goose astrovirus.","authors":"Linxiang Zheng, Huanrong Zhang","doi":"10.30466/vrf.2024.2034344.4346","DOIUrl":"10.30466/vrf.2024.2034344.4346","url":null,"abstract":"<p><p>China's burgeoning animal husbandry sector has witnessed a notable expansion in goose farming. Among the various health challenges, a novel goose astrovirus (GoAstV) has emerged as a significant threat to the industry, necessitating prompt detection strategies to mitigate its economic impact. This research introduces a novel detection approach using real-time fluorescence-based reverse transcription recombinase-aided amplification (RT-RAA), offering a rapid and reliable method for GoAstV identification. We meticulously designed specific primers and probes, and optimized the RT-RAA reaction conditions. The assay's specificity, sensitivity, repeatability, and clinical efficacy were rigorously assessed. Our method achieves detection within a swift 26-min window at a constant temperature of 39.00 ˚C, boasting a detection threshold as low as 1.19 × 10² copies <i>per</i> μL. Notably, the assay exhibited no cross-reactivity with closely related viruses, including Newcastle disease virus, avian influenza virus H9 subtype, goose circovirus, goose parvovirus, duck Tembusu virus, and avian adenovirus type 4. Validation through testing of 40 clinical samples confirmed a 100% agreement with pre-existing data. The study's outcomes underscore the high specificity, sensitivity, and operational simplicity of the developed RT-RAA assay, positioning it as an ideal candidate for the rapid and on-site detection of GoAstV.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"309-316"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and molecular characterization of resistant <i>Staphylococcus aureus</i> strains in bulk milk tanks of dairy cattle in Northern Egypt.","authors":"Walaa Abdallah Gad, Salama Ahmed Osman, Khaled Abd El-Hamid Abd El-Razik, Ashraf Hassan Soror, Yousef Adel Soliman, Ehab Ali Fouad","doi":"10.30466/vrf.2024.2038109.4398","DOIUrl":"10.30466/vrf.2024.2038109.4398","url":null,"abstract":"<p><p>Bovine subclinical mastitis represents a major cause of severe economic losses in dairy farms. This research aimed to detect the antimicrobial resistance trends of <i>Staphylococcus aureus</i> and to determine the presence of <i>mecA,</i> <i>mphC, lnuA, tetK</i> and <i>tetL</i> antimicrobial resistance genes in raw bulk milk in the period between December 2023 and February 2024. One hundred raw bulk cow milk samples were gathered from different dairy farms in Egypt. The prevalence of subclinical bovine mastitis was 65.00% using California mastitis test. The prevalence of isolated <i>S. aureus</i> was 46.15% via bacterial culturing and all isolates (n = 30) were confirmed via hemolytic activity, catalase and coagulase test, and gram staining followed by polymerase chain reaction targeting <i>nuc1</i> gene. Antimicrobial sensitivity test was applied on all confirmed <i>S. aureus</i> isolates utilizing the disk diffusion method on Mueller-Hinton agar. The highest resistance was verified for tetracycline at 100% followed by erythromycin and clindamycin at 56.66 and 16.66%, respectively. The highest sensitivity at 100% was verified for amikacin, ampicillin, amoxicillin plus clavulanic acid, ampicillin plus sulbactam, ciprofloxacin, colistin, gentamicin, imipenem, tobramycin, doxycycline and vancomycin. Multidrug resistance was found in 20.00% of the total isolates. Methicillin resistant <i>S. aureus</i> represented by <i>mecA</i> gene was identified in 83.33% of isolates. Macrolides resistant <i>S. aureus</i> represented by <i>mphC</i> gene was identified in 16.66% of isolates. Lincosamide resistant <i>S. aureus</i> represented by <i>inuA</i> gene was identified in 66.66% of isolates. Tetracycline resistant <i>S. aureus</i> represented by <i>tetK</i> and <i>tetL</i> genes was detected in 23.33 and 53.33% of isolates, respectively. This study provided antibiotic-resistant <i>S. aureus</i> profiles to dairy farms to avoid treatment failure, adverse effects on animal health and economic impact for the owner of the animal.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"317-323"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the effect of <i>Tarantula cubensis</i> alcoholic extract on cadmium embryotoxicity.","authors":"Rahmi Canbar, Gokhan Akcakavak, Muhittin Uslu, Mustafa Sedat Arslan, Harun Kizilay","doi":"10.30466/vrf.2024.2031444.4313","DOIUrl":"10.30466/vrf.2024.2031444.4313","url":null,"abstract":"<p><p><i>Tarantula cubensis</i> alcoholic extract (TCAE) is a homeopathic product used in the veterinary field. This study aimed to determine the effects of TCAE on cadmium (Cd) toxicity in the embryo. The study used 220 fertile, incubated chicken eggs divided into 11 equal groups on the 7^th day of incubation. The groups comprised untreated and physiological saline control groups, a group with TCAE alone, four groups with varying doses of Cd alone and four groups with the same doses of Cd plus TCAE. At the end of the incubation period, the eggs were opened, kidney and liver tissue samples were taken for histopathology and the number of dead and living embryos were recorded. In the prsent study, the median lethal dose of Cd was determined to be 0.029 mg <i>per</i> egg and the median lethal dose of Cd plus TCAE was determined to be 0.020 mg <i>per</i> egg. The histopathological examinations determined that kidney and liver damage were increased when TCAE and Cd were administered together, that was higher than when Cd was given alone. Thus, TCAE, which had no toxic effect on the embryo when used alone, might increase the embryotoxic activity of Cd. However, more detailed studies are needed.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"203-208"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Gundelia tournefortii</i> extract on diabetic gastropathy: involvement of inflammation, apoptosis, oxidative stress, and histopathology.","authors":"Muhammet Bahaeddin Dörtbudak, Uğur Şeker, Muhammet Demircioğlu, Ismail Demircioğlu","doi":"10.30466/vrf.2024.2027690.4249","DOIUrl":"10.30466/vrf.2024.2027690.4249","url":null,"abstract":"<p><p>In this study, the effect of <i>Gundelia tournefortii</i> (GT) extract against diabetic gastropathy was investigated by pathological methods. The animal groups were designed as the control, diabetes, diabetes + GT50, diabetes + GT100, and diabetes + GT200 groups. No treatment was applied to the control group. The other groups received 45.00 mg kg^-1 streptozotocin intraperitoneally on the experimental day. The treatment groups were also given 50.00, 100, and 200 mg kg^-1 of GT extract daily by gavage for 21 days. Tissues were stained with Hematoxylin and Eosin for histopathological examination. Immunohistochemical staining was performed to reveal the presence of inflammation (tumor necrosis factor alpha), apoptosis (cysteine aspartate specific proteases-3), and oxidative stress (heat shock protein-27). Histopathological examination revealed no pathological lesion in the control group. In the diabetes group, mucosal tissue damage, and vascular and inflammatory changes were observed. In the treatment groups, GT decreased histopathological findings in parallel with the dose increase. Immunohistochemical examination revealed no immunopositivity in the control group, while severe immunopositivity was observed in the diabetes groups in terms of inflammation, apoptosis, and oxidative stress. In the treatment groups, there was a decrease in the severity of immunopositivity's depending on the dose increase. As a result of this study, which has not been done before, GT was found to have a protective effect against gastropathy, being an important complication of diabetes, and this study is thus an important reference point for future research and promises new hope for the patients.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 3","pages":"133-139"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent occurrence of metastatic cutaneous melanoma and early feline diffuse iris melanoma in a cat.","authors":"Yesari Eroksuz, Sumeyye Babacan, Eren Polat, Mustafa Issi, Necati Timurkaan, Burak Karabulut, Elif Ekinci","doi":"10.30466/vrf.2024.2037225.4385","DOIUrl":"10.30466/vrf.2024.2037225.4385","url":null,"abstract":"<p><p>This report presented a case of the concurrent diagnosis of early feline diffuse iris melanoma (FDIM) and metastatic cutaneous melanoma in a tricolored mixed stray cat. Histopathological examination of biopsy samples identified melanoma on the nasal planum, lips along with metastasis to the submandibular lymph nodes. The initial treatment included surgical resection of the primary nasal planum neoplasm and the metastatic lesions in the submandibular lymph nodes. The necropsy, performed 40 days after the surgery, revealed the metastatic nodules in multiple organs including the liver, lungs, thyroids, pericardium, pleura, peritoneum, kidneys, and numerous lymph nodes. Histopathological examination revealed a biphasic neoplastic tissue composed of a mixture of epithelioid and spindle cells in primary and metastatic tumors. Additionally, the anterior surface of the iris contained 4 - 5 rows of melanocytic interstitial infiltration indicative of early FDIM. Immunohistochemical analysis revealed that the neoplastic cells showed positive immunoreactivity for Melan-A. To the authors' knowledge, this case represented the first reported case of concurrent FDIM and cutaneous melanoma in a feline patient.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 8","pages":"481-484"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of cage density and meat storage period on some meat quality parameters in brown and white spent hens.","authors":"Uğur Özentürk, Ayşe Uysal, Ekrem Laçin, Soner Uysal, Sevda Urçar Gelen, Hayrunnisa Özlü","doi":"10.30466/vrf.2024.2025425.4210","DOIUrl":"10.30466/vrf.2024.2025425.4210","url":null,"abstract":"<p><p>This study was conducted to determine the effect of different cage densities and meat storage periods on some meat quality parameters in drumstick and breast meat of brown and white spent hens. The study used three different cage densities as low (5 hens <i>per</i> cage), normal (seven hens <i>per</i> cage) and high (10 hens <i>per</i> cage) including 396 hens as 198 Hy-Line Brown (HB) and 198 Isa Tinted (IT). The feeding of chickens was <i>ad libitum</i> (20 - 60 weeks). At the end of the study, a total number of 54 chickens (27 HB and 27 IT) were slaughtered. Some chemical and microbiological analyses were carried out by separating the drumstick and breast area of the slaughtered chickens. According to the results of the research, the redness and yellowness values of the breast area were higher in the ITs. The effect of cage density was significant only for yellowness in the drumstick area. The effects of storage time, lightness and yellowness in the drumstick area and lightness value in the breast area were found to be significant. Bacterial density and thiobarbituric acid reactive substances values of both breast and drumstick regions of spent hens were higher in HBs, while pH values were higher in ITs. The bacterial density in the meat was increased during the storage period. In conclusion, cage density, genotype and storage time affected the microbiological and chemical quality of spent chicken meat. With this result, it can be said that meat quality studies, which mostly focus on broilers, are also important in spent hens.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 2","pages":"71-79"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of erythropoietin on cardiac morphometry in exercised male and female adolescent rats.","authors":"Mehmet Burak Ateş, Gokhan Akcakavak, Ozgur Ozdemir, Mehmet Ozdemir, Ibrahim Bozkurt","doi":"10.30466/vrf.2024.2035267.4360","DOIUrl":"10.30466/vrf.2024.2035267.4360","url":null,"abstract":"<p><p>Erythropoietin (EPO) is a glycoprotein hormone predominantly produced in the kidneys, primarily stimulating erythroid cell proliferation in the bone marrow. The present study investigated the impact of EPO combined with swimming exercise on cardiac morphometry in adolescent male and female rats. The 4-week study involved 48 rats (24 males and 24 females), which were divided into four main groups of six males and six females each. The control group was administered intraperitoneal saline four times a week. The swimming exercise group also received intraperitoneal saline, followed by 30 min of swimming exercise, four times a week. The drug control group was given 50.00 IU kg^-1 epoetin alfa intraperitoneally, four times a week. Lastly, the Swimming + Drug group received 50.00 IU kg^-1 epoetin alfa intraperitoneally, four times a week, followed by 30 min of swimming exercise. The post-study measurements demonstrated that EPO administration did not result in notable alterations in crucial parameters, including the left ventricular mass index, left ventricular mass, and left ventricular posterior wall in the context of left ventricular hypertrophy in both genders. However, in female rats, EPO-only group and the combined EPO and exercise group showed significant thinning of the right ventricular wall and interventricular septum indicating potential cardiac dilatation. The results highlight the necessity of considering gender-specific responses when evaluating EPO's cardiovascular effects, particularly concerning the right ventricle, and suggest further investigation into the long-term consequences of these observed changes.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"189-194"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of effects of vancomycin/polycaprolactone nanocomposite in comparison with curcumin/polycaprolactone on the healing of experimental osteomyelitis in rabbit tibia.","authors":"Amirreza Hajati Ziabari, Alireza Jahandideh, Abolfazl Akbarzadeh, Pejman Mortazavi","doi":"10.30466/vrf.2024.2028501.4268","DOIUrl":"10.30466/vrf.2024.2028501.4268","url":null,"abstract":"<p><p>Osteomyelitis is caused by the local spread of an infected source adjacent to the infection following trauma, bone surgery or joint replacement. The present study aimed to evaluate the effect of vancomycin (Van)/polycaprolactone (PCL) nanocomposite in comparison with curcumin (Cur)/PCL on the healing of experimental osteomyelitis in tibia in rabbits. After induction of osteomyelitis forty adult male New Zealand white rabbits were randomly divided into four groups. Control group: The animals were considered as controls and no scaffolds were used. In PCL/Van group, the created bone defects were filled with the combination of PCL and Van. In PCL/Cur, the created bone defects were filled with the combination of PCL and Cur. Polycaprolactone/Cur/Van group: The created bone defects were filled with the combination of PCL, Cur and Van. Bone samples were taken for histopathological evaluation on the 30^th and 60^th days. For radiological evaluations of the osteomyelitis, radiographs were prepared at time intervals zero (day of surgery), 15, 30, 45, and 60 days after surgery. In order to evaluate hematology, blood was taken on days 0 (day of surgery), 15, 30, 45, and 60. The results of the present study showed that Cur nanocomposite significantly improved the healing process of the rabbit tibia experimental osteomyelitis model compared to the control group. Also, the PCL/Cur/Van group showed the best healing results. In conclusion, PCL/Cur/Van nanocomposite scaffold showed positive effects on the healing process.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 4","pages":"235-243"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper and zinc dynamics in ovine pneumonia: a comparative analysis of treatment regimens.","authors":"Amir Ganjkhanloo, Bahram Dalir-Naghadeh, Ghader Jalilzadeh-Amin, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2043423.4496","DOIUrl":"10.30466/vrf.2024.2043423.4496","url":null,"abstract":"<p><p>Pneumonia remains a significant economic burden on the small ruminant industry. Excessive inflammation, oxidative stress, and alterations in copper and zinc can accompany pneumonia. As these micro-nutrients play crucial roles in immune function and anti-oxidant defence, modulating their levels may influence the disease progression. This study aimed to investigate the effects of different therapeutic regimens on copper and zinc status in lambs with pneumonia. Twenty lambs with pneumonia were randomly assigned to four treatment groups: oxytetracycline and tylosin (OT), OT plus vitamin B1 (OTVB1), OT plus vitamin C (OTVC), and OT plus vitamin B1 and vitamin C (OTVB1C). A control group received only distilled water. Blood samples were collected on days 1, 3, 6, and 14 for subsequent assessment of plasma copper and zinc concentrations. While the control group maintained stable levels, the pneumonic groups exhibited varying degrees of changes. Plasma copper concentrations increased significantly in all pneumonic groups compared to the control group throughout the study. The OT and OTVB1C groups had the highest number of lambs with increased copper level. Plasma zinc concentrations decreased significantly in the OT and OTVB1 groups, with the lowest levels in the OTVB1 group on day 3. The OTVC group mirrored the control group with stable levels. The OTVB1C group, compared to the other groups, showed a more persistent reduction. These findings suggest that the effects of the various treatment regimens on plasma copper and zinc levels may be complex and time-dependent.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"16 6","pages":"331-338"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12295534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}