Veterinary Research Forum最新文献

{"title":"Frequency of <i>Anaplasma platys</i> in dogs from the municipality of Veracruz, Mexico.","authors":"Izcalli Alejandra Jeréz-Sulvarán, David Itzcóatl Martínez-Herrera, Héctor Vivanco-Cid, José Alfredo Villagómez-Cortés, Héctor Alejandro Contreras-López, Lucy Anahi Muñoz-Muñoz, José Luis Hernández Vivanco, Argel Flores Primo","doi":"10.30466/vrf.2024.2021313.4154","DOIUrl":"10.30466/vrf.2024.2021313.4154","url":null,"abstract":"<p><p>Infectious canine cyclic thrombocytopenia or canine anaplasmosis is an infectious disease caused by <i>Anaplasma platys</i>. In Mexico, cases of human infection have been reported. The present cross-sectional study aimed to determine the frequency of <i>A. platys</i> infection in the municipality of Veracruz, Mexico, by nested polymerase chain reaction method. A total of 100 blood samples from dogs living in the municipality were collected and analyzed between March and June 2022. A descriptive analysis of blood samples for hemoparasites frequencies was performed with the free online software VassarStats. The evaluated variables were sex, street access, usage of ticks control methods, and living environment. The free online software WinEpi software was used to calculate odds ratio (OR) and confidence interval (CI: 95.00%). Out of 100 analyzed blood samples, 27 were confirmed positive for <i>A. platys</i>. The only risk factor found was the absence of tick prevention methods (OR = 9.81; 95.00% CI: 23.00 - 44.50). In conclusion, the frequency of <i>A. platys</i> was 27.00% and no risk factors were identified.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"697-700"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of gamma irradiation and disaccharide trehalose to induce immune responses in Syrian hamster model against Iranian SARS-CoV-2 virus isolate.","authors":"Farahnaz Motamedi-Sedeh, Akbar Khorasani, Mohsen Lotfi, Seyed Morteza Moosavi, Arash Arbabi, Seyedeh Maede Hosseini","doi":"10.30466/vrf.2024.2022838.4172","DOIUrl":"10.30466/vrf.2024.2022838.4172","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is the causative agent of the emerging zoonotic respiratory disease. One of the most important prerequisites for combating emerging diseases is the development of vaccines within a short period of time. In this study, antigen-irradiated, inactivated SARS-CoV-2 viruses and the disaccharide trehalose were used to enhance immune responses in the Syrian hamster. The SARS-CoV-2 virus was isolated from tracheal swabs, confirmed by real-time polymerase chain reaction, and propagated on Vero cells. For inactivation, it was irradiated with 14.00 kGy gamma radiation. Evaluation of the antigenic properties of the spike protein subunit S1 showed that the antigens were intact after gamma irradiation. The gamma-irradiated and formalin-treated viruses were used to immunize hamsters in four vaccine formulations. Neutralizing antibodies increased significantly in all vaccinated groups three weeks after the second and third vaccinations. The concentration of secretory immunoglobulin A in the irradiated vaccine plus trehalose increased significantly in nasal lavage and nasopharyngeal-associated lymphoid tissue fluids three weeks after the second and third vaccinations. The lymphocyte proliferation test in the spleen showed a significant increase in all vaccinated hamsters, but the increase was greater in irradiated vaccine plus trehalose and irradiated vaccine plus alum. We can recommend the irradiated inactivated vaccine SARS-CoV-2 plus trehalose (intra-nasal) and another irradiated inactivated vaccine SARS-CoV-2 plus alum (subcutaneous) as safe vaccines against coronavirus disease of 2019 (COVID-19), which can stimulate mucosal, humeral, and cellular immunities. However, the protectivity of the vaccine against COVID-19 in vaccinated hamsters must be investigated in a challenge test to assess the potency and efficiency of vaccine.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"681-689"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of curcumin-loaded polycaprolactone scaffold on Achilles tendon repair in rats.","authors":"Narges Sufian, Mehdi Behfar, Rahim Hobbenaghi, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2029563.4286","DOIUrl":"10.30466/vrf.2024.2029563.4286","url":null,"abstract":"<p><p>Scaffolds play a crucial role in tendon healing by providing structural support, promoting cell infiltration, and guiding tissue regeneration. Polycaprolactone (PCL) has been used as a polymer in biological scaffolds for several tissue engineering studies. This study aimed to investigate the effects of curcumin-loaded PCL scaffold on Achilles tendon using a tenotomy model in rats. Twenty adult male Wistar rats were randomized into two groups. In control group, tenotomy and suture placement were performed. The identical intervention followed by the implantation of curcumin-loaded PCL scaffold around the tendon stumps was performed in the treatment group. The nanofibrous PCL scaffold containing 5.00% curcumin was fabricated by electrospinning. Walking track analysis was performed weekly. Then, after 6 weeks, histopathological examination and tendon mechanical tests were performed. The weekly walking track analysis revealed a significant improvement in Achilles functional index in scaffold-treated rats from week three to six. The rate of functional improvement was remarkably slower in the control group. Histopathological examination revealed aseptic inflammation and enhanced neovascularization in the treatment group. Also, collagen arrangement and density were significantly improved in this group compared to the control samples including less regular orientation and loose organization of collagen fibers. Significant increase in mechanical properties, except for strain, was observed in the treatment group. The present study demonstrated that implantation of curcumin-loaded PCL scaffold resulted in increased fibrillar architecture, as well as improved mechanical properties and Achilles functional index in rats. To reduce the biodegradation-induced inflammation, an anti-inflammatory treatment is recommended.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"621-627"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis of oxidative stress indices and clinical parameters in budgerigars (<i>Melopsittacus undulatus</i>) treated with medetomidine-ketamine and midazolam-ketamine.","authors":"Zahra Amini, Fatemeh Hoseinpour, Ali Ghashghaii, Hadi Cheraghi","doi":"10.30466/vrf.2023.1999287.3836","DOIUrl":"10.30466/vrf.2023.1999287.3836","url":null,"abstract":"<p><p>Various companion birds, including budgerigars, are anesthetized with injectable anesthesia. The current study aimed to evaluate oxidative stress indices including malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) along with clinical parameters such as the time required to induce, maintain and recover from medetomidine-ketamine anesthesia and midazolam-ketamine anesthesia in budgerigars. Among 20 mature and healthy budgerigars, three groups were assigned as follows: Control (n = 4) to determine baseline oxidative stress indices medetomidine + ketamine (n = 8) anesthetized by intramuscular injections of medetomidine (0.04 mg kg^-1) and ketamine (30.00 mg kg^-1) in the pectoral muscles, midazolam + ketamine (n = 8) anesthetized by intramuscular injections of midazolam (1.00 mg kg^-1) and ketamine (50.00 mg kg^-1). Half of birds (n = 4) in the second and third groups were euthanized by cervical dislocation 1 hr after anesthesia induction, blood samples were collected directly from the heart, and sera were extracted. Additionally, the remaining birds were euthanized 24 hr later, and their serum was analyzed for oxidative stress indices. Clinical parameters were recorded during the study. Compared to the medetomidine + ketamine group, the midazolam + ketamine group experienced shorter induction, anesthetic, and recovery times. Administering medetomidine and ketamine elevated TOS levels compared with midazolam + ketamine. No significant difference was found between the test groups for TAC, MDA, or OSI. Therefore, the midazolam + ketamine regimen appears superior to medetomidine + ketamine when performing minor surgeries on budgerigars.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 5","pages":"237-242"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of multi-enzymes supplementation to wheat and soybean meal-based feeds on growth performance, digestibility and carcass characteristics of quails.","authors":"Mehmet Irmak, Muzaffer Denli, Veysi Kayri, İsa Coşkun","doi":"10.30466/vrf.2024.2008986.3971","DOIUrl":"10.30466/vrf.2024.2008986.3971","url":null,"abstract":"<p><p>Japanese quail (<i>Coturnix coturnix japonica</i>) is a popular experimental animal model in scientific research. The present study investigated the effects of dietary multiple enzyme supplementation on growth performance, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails fed diets based on wheat and soya bean meal. A total number of 192 1-day-old quails were assigned to three treatments with 16 replicates in each and four quails <i>per</i> replicate for 38 days. The control group received a basal diet, and the treatment groups received a basal diet with 0.10 or 0.20% multi-enzyme, respectively. Growth performance parameters, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails were evaluated. Dietary supplementation of multi-enzymes to diet significantly increased body weight gain and improved the feed conversion rate. Moreover, quails fed with 0.10 or 0.20% multi-enzymes showed better ash digestibility coefficients and apparent metabolizable energy coefficients than the control quails. Furthermore, quails fed on a diet containing 0.20% multi-enzyme had the highest crude fiber digestibility. The villi length and the villi length/crypt depth ratio of the duodenum were significantly increased and the crypt depth was decreased in quails-fed diets supplemented with both multi-enzyme levels. However, feed consumption, carcass yield, carcass weight, heart weight, gizzard weight, liver weight and total intestine weights were not affected by treatments. In conclusion, our results showed that dietary supplementation of multi-enzymes to a wheat and soybean meal-based diet enhanced the growth performance and nutrient digestibility of quails.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"583-590"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crimean-Congo hemorrhagic fever, transfer patterns, vectors and history in Iran and neighboring countries.","authors":"Mousa Tavassoli, Salar Zarrabi Ahrabi, Gurkan Akyildiz, Aysen Gargili Keles","doi":"10.30466/vrf.2024.2022437.4162","DOIUrl":"10.30466/vrf.2024.2022437.4162","url":null,"abstract":"<p><p>Crimean-Congo hemorrhagic fever (CCHF) is indeed to be considered as one of the most significant vector-borne diseases globally. The virus responsible for CCHF can persist in various animals and lead to severe infections in humans. Ticks of the <i>Ixodidae</i> family are the acknowledged vectors of CCHF virus (CCHFV) transmission to humans. In this review, different tick species from Iran and its neighbors and their roles in the transmission of CCHFV were evaluated. However, the transmission patterns of CCHF to humans primarily involve tick bites, direct contact with the blood, organs, or tissues of contaminated animals, and exposure to CCHF patients are important in virus transmission. The results of this review confirm that the tick vectors in Iran and its neighbors are very common. Even though tick bite is one of the most important risk factors for CCHF, it cannot explain all cases, and there are other important risk factors, such as high-risk occupations and having contact with livestock. Ticks that have been proven to transmit the disease, especially <i>Hyalomma</i> <i>marginatum</i>, are scattered in Iran and neighboring countries. Transporting passengers and livestock and sometimes smuggling livestock are the means of transmitting the virus between neighboring countries. Therefore, there is a need for joint preparedness and response programs to prevent and manage CCHF between Iran and its neighbors.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"575-582"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance patterns, characteristics of virulence and resistance genes and genotypic analysis of <i>Salmonella</i> serotypes recovered from different sources.","authors":"Seyed Mahdi Hosseini, Rahem Khoshbakht, Hami Kaboosi, Fatemeh Peyravii Ghadikolaii","doi":"10.30466/vrf.2024.2019507.4123","DOIUrl":"10.30466/vrf.2024.2019507.4123","url":null,"abstract":"<p><p>The present study evaluated the <i>Salmonella</i> isolates obtained from various origins in Iran. <i>Salmonella</i> strains previously recovered and stored in the veterinary microbiology laboratory were serotyped and subjected to antibiotic susceptibility test, detection of the virulence and resistance genes by polymerase chain reaction (PCR), and genotyping by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). All <i>Salmonella</i> isolates showed resistance to erythromycin and the most resistance rates were detected for trimethoprim (86.66%), ampicillin (75.00%), and sulfamethoxazole-trimethoprim (63.33%), respectively. In total, 86.33% of the isolates were known as multi-drug resistant and none of the isolates showed resistance to cefepime, nalidixic acid, imipenem, ceftriaxone, and polymyxin B. The virulence genes, <i>invA</i>, <i>sdiA</i>, and <i>hilA</i> besides the <i>tetA</i> resistance gene were identified in all 60 <i>Salmonella</i> strains. The most prevalent resistance genes were respectively <i>tetC</i> (70.00%), <i>sul2</i> (58.33%), and <i>ereA</i> (55.00%). Statistical analysis revealed a significant difference between <i>Salmonella</i> serotypes associated with the <i>sul1</i> resistance gene. In ERIC-PCR analysis, 14 distinct clusters were obtained. Statistically, there were significant relationships between the source and ERIC's genomic pattern and between the serotype of <i>Salmonella</i> isolates and genotypic pattern of ERIC. According to the results, <i>Salmonella</i> serotypes from non-human sources had considerable resistance to different antibiotics and carried significant virulence determinants and resistance genes. In addition, ERIC-PCR showed relevant results in discriminating <i>Salmonella</i> serotypes from other sources.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"499-508"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens.","authors":"Hossein Alavizadeh, Zahra Moosavi, Gholamreza Hashemi Tabar","doi":"10.30466/vrf.2024.2023088.4174","DOIUrl":"10.30466/vrf.2024.2023088.4174","url":null,"abstract":"<p><p>Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran. The dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia and necrotizing enteritis were some of the findings reported in previous research. Low macrophage infiltration rate and brain lesions were other discoveries in Hematoxylin and Eosin (H&E) examination. Feulgen reaction and Cluster of Differentiation 68 (CD68) immunohistochemical staining were used for a comprehensive investigation and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggested that HPS might cause brain damage and the susceptibility of the Arian breed to the adenovirus needs further investigation.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"559-564"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia.","authors":"Ozhan Karatas, Gokhan Akcakavak","doi":"10.30466/vrf.2024.2023580.4184","DOIUrl":"10.30466/vrf.2024.2023580.4184","url":null,"abstract":"<p><p>Contagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1β protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immuno-histochemical examination, MC profile and local IL-17 and IL-1β protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1β scores were detected in the peracute-acute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1β expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course. Additionally, MC profile was evaluated for the first time in naturally infected CCPP.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"515-521"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic potential of autochthonous <i>Lactobacillus</i> species from buffalo calves in controlling multidrug resistant <i>Escherichia coli</i>.","authors":"Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu","doi":"10.30466/vrf.2024.2012559.4024","DOIUrl":"10.30466/vrf.2024.2012559.4024","url":null,"abstract":"<p><p>The aim of this study was to investigate the probiotic potential of autochthonous <i>Lactobacillus</i> species isolated from buffalo calves against multidrug-resistant <i>Escherichia</i> <i>coli</i>. A total of 252 rectal swabs were collected from healthy neonatal buffalo calves under 30 days old from six districts of Andhra Pradesh, India in a completely randomized design from August 2019 to August 2021, of which 190 <i>Lactobacillus</i> strains were isolated based on cultural, morphological, biochemical and molecular tests. Out of 190 isolates, 57 showed high levels of auto-aggregation (> 80.00%) and hydrophobicity (> 60.00%) and 51 of the 57 isolates had a zone of inhibition greater than 15.00 mm in diameter against multidrug-resistant <i>E. coli</i> in an Agar well diffusion assay. Among the 51 isolates, 36 were found to be acid and bile tolerant and showed varying levels of sensitivity to antibiotics such as erythromycin, clindamycin, tetracycline, chloramphenicol, and ampicillin. Among the 36 isolates, <i>Limosilactobacillus reuteri</i> <i>178</i>, <i>L. reuteri</i> <i>209</i>, <i>L. fermentum 182</i>, <i>L. fermentum</i> <i>211</i>, and <i>Lactiplanti-bacillus plantarum</i> <i>34</i> were non-hemolytic, and none of the isolates were able to hydrolyse gelatine. Therefore, these five autochthonous <i>Lactobacillus</i> species may be used in probiotic or synbiotic formulations against multidrug resistant <i>E. coli</i> in buffalo calves.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"275-282"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}