Veterinary Research Forum最新文献_第6页

Occurrence, characteristics, and antibiotic sensitivity of Staphylococcus aureus isolated from wild birds in the Kasur district of Punjab, Pakistan. 从巴基斯坦旁遮普省卡苏尔地区野鸟体内分离出的金黄色葡萄球菌的发生率、特征和抗生素敏感性。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2024.2009978.3990

Tooba Latif, Shahzad Ali, Arshad Javid, Ali Ahmad Sheikh

{"title":"Occurrence, characteristics, and antibiotic sensitivity of Staphylococcus aureus isolated from wild birds in the Kasur district of Punjab, Pakistan.","authors":"Tooba Latif, Shahzad Ali, Arshad Javid, Ali Ahmad Sheikh","doi":"10.30466/vrf.2024.2009978.3990","DOIUrl":"10.30466/vrf.2024.2009978.3990","url":null,"abstract":"Staphylococcus aureus is gaining worldwide attention because of its substantial impact on public health. The current study aimed to characterize S. aureus strains isolated from wild birds in the Kasur district of Punjab, Pakistan from 2021 to 2022. A total of one hundred samples were collected from five wild bird species. The samples were enriched, inoculated on selective agars and cultured for 24 hr at 37.00 ˚C. All isolates were verified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) after Gram staining. Positive isolates were screened for phenotypic (Kirby-Bauer disk diffusion and minimum inhibitory concentration s), genotypic antibiotic resistance, and virulence genes. These samples yielded 30 (30.00%) S. aureus isolates, confirmed by polymerase chain reaction utilizing the 16S rRNA gene. Staphylococcus aureus was more prevalent in cloacal samples (16.00%) than oral samples (14.00%). Various S. aureus isolates showed varying degrees of resistance to three different antibiotics. Oxacillin (56.66%; n = 17) and tetracycline (33.33%; n = 10) showed the highest resistance rates with the lowest susceptibility (43.33%; n = 13). In contrast, vancomycin, rifampicin, linezolid, and daptomycin were 100% susceptible. Further disc diffusion study revealed resistance to tetracycline (33.33%), erythromycin (16.66%), and gentamicin (10.00%). The tetK gene was found in 33.33% of wild bird samples, while the ermA gene was found in 16.66% of samples. The aacA-D gene was only found in three (10.00%) isolates. None of the isolates tested positive for virulence genes. In conclusion, S. aureus is carried by wild birds in this area, posing a potentail threat to both humans and animals.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"283-290"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of pigeon- origin avian avulavirus-1 reveals unique substitutions in F and HN proteins. 源于鸽子的禽流感病毒-1 的遗传特征揭示了 F 蛋白和 HN 蛋白的独特替代。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI: 10.30466/vrf.2023.2004035.3904

Deepthi Balam, Ratnamma Doddamane, Ramani Pushpa Rn, Shrikrishna Isloor, Veeregowda Bm

{"title":"Genetic characterization of pigeon- origin avian avulavirus-1 reveals unique substitutions in F and HN proteins.","authors":"Deepthi Balam, Ratnamma Doddamane, Ramani Pushpa Rn, Shrikrishna Isloor, Veeregowda Bm","doi":"10.30466/vrf.2023.2004035.3904","DOIUrl":"10.30466/vrf.2023.2004035.3904","url":null,"abstract":"Since decades, Newcastle disease (ND) has become endemic in the poultry population of the Indian subcontinent. ND is a highly contagious disease of poultry and other avian species. However, the genetic nature of ND viruses circulating in the rock pigeons is unraveled. The present investigation is a part of Newcastle disease virus (NDV) surveillance in wild birds. Two velogenic NDV strains could be isolated from apparently healthy rock pigeons, thus establishing the status of carrier/reservoir host. The fusion protein cleavage site in the fusion protein has multiple basic amino acid (RRRKRF) motifs similar to velogenic isolates. Phylogenetic analysis based on complete fusion gene sequences confirmed that the isolates belong to NDV sub genotype XIII 2.2. Further analysis revealed several amino acid substitutions in the hypervariable region, heptad repeat regions and neutralizing epitopes of the fusion protein and heptad repeat regions and antigenic sites of the hemagglutinin-neuraminidase (HN) protein that are critical for fusion. A unique D170A substitution in the neutralizing epitope is identified that is critical for structure and function of the fusion protein. Mutations within the virulence determinants including fusion (F) and HN, elucidate continuous evolution of the viruses among the rock pigeons. Accidental spillover of these mutated viruses into commercial poultry operations may result in disease outbreaks with economic breakdown.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 6","pages":"317-323"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of captopril on paraplegia caused by spinal cord ischemia-reperfusion injury in rats. 卡托普利对大鼠脊髓缺血再灌注损伤所致截瘫的影响

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2019729.4126

Bahareh Hafezi, Hossein Kazemi Mehrjerdi, Amir Moghaddam Jafari

{"title":"Effect of captopril on paraplegia caused by spinal cord ischemia-reperfusion injury in rats.","authors":"Bahareh Hafezi, Hossein Kazemi Mehrjerdi, Amir Moghaddam Jafari","doi":"10.30466/vrf.2024.2019729.4126","DOIUrl":"https://doi.org/10.30466/vrf.2024.2019729.4126","url":null,"abstract":"This study investigated the effect of captopril (Cap) on spinal cord ischemia-reperfusion injury (SCII) in rats. Twenty-four adults male Wistar rats were randomly divided into four groups of six animals each: spinal cord ischemia-reperfusion (SCI-R) with Cap (SCI-R + Cap), SCI-R, sham-operated with Cap (SHAM + Cap), and SHAM. The 24 hr and 90 min before ischemia induction, Cap was administered intragastrically (100 mg kg-1) to the SHAM + Cap and SCI-R + Cap groups. Abdominal aortic clamping was performed in the SCI-R and SCI-R + Cap groups for 40 min. Hindlimb motor function was evaluated using the Tarlov Scale at 4, 6, 12, 24, 48, and 60 hr after SCII. The malondialdehyde (MDA), the ferric-reducing ability of plasma (FRAP) and prooxidant-antioxidant balance (PAB) values were also measured. Throughout the study period, the SCI-R group had significantly lower motor function scores compared to the other groups. The MDA and PAB levels were higher and the FRAP value was lower in the SCI-R group compared to in the SHAM group. The SCI-R + Cap had higher motor function scores compared to the SCI-R group at all time points. There were no significant differences in MDA concentration, FRAP and PAB values between the SCI-R + Cap and SCI-R groups. Captopril may act as a protective agent against SCII in rats based on hind limb motor function assessment.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 7","pages":"379-385"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens. 超滤纯化传染性法氏囊病病毒对特定无病原体鸡免疫反应和细胞因子活化的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI: 10.30466/vrf.2023.2009350.3978

Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili

{"title":"Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens.","authors":"Shahla Shahsavandi, Mohammad Majid Ebrahimi, Ali Nazari, Iraj Khalili","doi":"10.30466/vrf.2023.2009350.3978","DOIUrl":"10.30466/vrf.2023.2009350.3978","url":null,"abstract":"Purification is an important step in the production of viral vaccines that strongly affects product recovery and subsequent immune responses. The present study was carried out with the aim of improving the purification of infectious bursal disease virus (IBDV) by the tangential flow filtration (TFF) method. Then, the effect of the purified virus on the induction of immune responses against IBDV in specific pathogen free (SPF) chickens was investigated. The IBD07IR strain was propagated in embryonated SPF eggs. The virus was purified using a 100 kDa cassette. The quality of the recovered viruses was evaluated by titration. A total number of 60 SPF chickens were randomly divided into three groups (n = 20) and received the concentrated viral antigen, commercial live IBDV vaccine and phosphate-buffered saline at the age of 3 weeks by eye drop method. The bursa of Fabricius was examined histopathologically for possible changes. Sera were collected at 1-week intervals from day 0 until the end of 6 weeks after vaccination. The IBDV-specific antibody levels, induction of cell-mediated immunity and mRNA expression levels of cytokines were evaluated. The results showed that despite a relative raise in virus titer from 7.66 to 8.17 embryo infectious dose (EID)50 mL-1 following purification, both the purified IBDV and commercial vaccine are able to induce strong immune responses against the virus. Within a context of egg-based IBDV vaccine production, a single-step TFF can be applied for the relatively purification. This platform requires a further study in the selection of multiple membranes to optimize the operating conditions and final product.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"49-55"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell proliferation and apoptosis in canine oral papillomatosis. 犬口腔乳头状瘤病的细胞增殖和凋亡。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30466/vrf.2023.1996086.3818

Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz

{"title":"Cell proliferation and apoptosis in canine oral papillomatosis.","authors":"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz","doi":"10.30466/vrf.2023.1996086.3818","DOIUrl":"10.30466/vrf.2023.1996086.3818","url":null,"abstract":"This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 2","pages":"75-82"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tonsillar squamous cell carcinoma in a male Lhasa Apso. 一只雄性拉萨阿普索犬的扁桃体鳞状细胞癌。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-07-15 DOI: 10.30466/vrf.2024.2017462.4096

Alaleh Rakhshanpour, Sayyed Jafar Hasani, Ali-Asghar Tehrani, Mehdi Behfar

引用次数: 0

Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow. 揭示奶牛子宫纵隔肌瘤声像图和组织病理学的综合探索。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2018975.4112

Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar

{"title":"Comprehensive exploration unveiling the sonography and histopathology of uterine leiomyoma in a cow.","authors":"Pururava Sharma, Akshay Sharma, Pravesh Kumar, Ankit Kumar Ahuja, Pankaj Sood, Alisha Dhiman, Rakesh Kumar","doi":"10.30466/vrf.2024.2018975.4112","DOIUrl":"10.30466/vrf.2024.2018975.4112","url":null,"abstract":"Genital tumours are rare among cattle, largely due to their relatively short lifespans. Leio-myoma, a smooth muscle tumour being more prevalent in dogs, appears only at a rate of 1.00 - 2.00% in cattle, affecting reproductive efficiency in cases of complete uterine obstruction. This case report involves an 8-year-old cow with repeated insemination attempts unveiled 5.00 cm intra-luminal uterine mass, obstructing the right uterine horn. Transrectal sonography (TRUS) revealed a highly vascularized mass with normal ovarian function. Confirmation of clinical condition, i.e., uterine leiomyoma, via uterine biopsy concluded the presence of neoplastic smooth muscle cells arranged in interlacing bundles showing mild pleomorphism, and special staining using Masson's trichrome revealed an unappreciable amount of connective tissue; subsequently right flank celiotomy was performed to remove the benign tumour. Forty-five days after celiotomy, TRUS examination confirmed an unobstructed uterine horn, and bilateral oviduct patency was adjudged with 2.50% methylene blue. Following treatment for chronic endometritis, artificial insemination led to conception nearly 90 days post-procedure. The TRUS aids preliminary diagnosis, while definitive identification demands necropsy and surgical methods. This case underscores the diagnostic significance of TRUS, histopathology and celiotomy for identifying and managing uterine leiomyoma in cattle.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"207-210"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam. 氯胺酮、异丙酚和异氟烷对使用美托咪啶和咪达唑仑进行预处理的临床健康犬心电图变量的影响。

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2024.2008055.3954

{"title":"Effects of ketamine, propofol and isoflurane on electrocardiographic variables in clinically healthy dogs premedicated with medetomidine and midazolam.","authors":"","doi":"10.30466/vrf.2024.2008055.3954","DOIUrl":"10.30466/vrf.2024.2008055.3954","url":null,"abstract":"The purpose of this study was to investigate the effects of three anesthetic agents, with premedication of medetomidine and midazolam, on electrocardiographic variables in dogs. Ten adult mixed breed dogs were used in a crossover design study, where they received ketamine, propofol and isoflurane treatments with a one-week washout period between them. In all three groups, medetomidine was administered first followed by midazolam after 15 min. Then, after 20 min, group 1 received ketamine intravenously (IV), group 2 received propofol (IV), and group 3 received isoflurane (inhalation). In all dogs, electrocardiographs were taken before and after premedication's, as well as every 15 min during anesthesia. Medetomidine significantly decreased heart rate and P wave amplitude and increased PR interval, R wave amplitude, QT interval, and T wave amplitude. Midazolam increased the amplitude of the R and T waves. Ketamine increased the heart rate and PR interval. Propofol increased the heart rate for up to 15 min, decreased the PR interval for up to 30 min, and the QT interval for up to 45 min. Isoflurane increased the heart rate and decreased the amplitude of R and T waves. The results showed that the drugs used in this study did not have many side effects on electrocardiographic variables and could be used without serious concern. The most important side effects observed were a severe reduction in heart rate and 1st degree atrioventricular (AV) block and, to a lesser extent, 2nd degree AV block caused by medetomidine and midazolam which were masked by the anesthetics.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"187-194"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test. 根据粪便幼虫数量减少试验评估阿苯达唑和左旋咪唑对羊群肺线虫的抗药性

IF 1 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.30466/vrf.2023.2010062.3991

Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin

{"title":"Evaluation of drug resistance to albendazole and levamisole against lung worms in goat flocks based on fecal larvae count reduction test.","authors":"Sepideh Abdolahzadeh, Mousa Tavassoli, Bijan Esmaeilnejad, Ghader Jalilzadeh-Amin","doi":"10.30466/vrf.2023.2010062.3991","DOIUrl":"10.30466/vrf.2023.2010062.3991","url":null,"abstract":"The over-use of anti-parasitic compounds as a method of control has led to insufficient effectiveness and widespread drug resistance worldwide. The aim of this study was to investigate the efficacy of albendazole and levamisole as anti-parasitic agents in a lung worm control program in goat flocks. During 2021 and 2022, a total of 110 goats (age of four months and above) were randomly selected from 11 herds in the north-western region of Iran including Saanen breed (both sexes of the same age). The results indicated that 3.60, 50.80 and 41.90% were respectively infected with Dictyocaulus filaria, Muellerius capillaris and Proto-strongylus rufescens, and generally all the lung parasites in goats of this region were resistant to albendazole and levamisole. Due to clinical importance of D. filaria in goats, the molecular analysis of two samples was also done. Sequencing results showed that the identified parasites were 100% similar to the reference sequences registered in the GenBank®. The results of this research showed low level of these anthelmintics efficacy against Dictyocaulus and Muellerius. Generally, the lung parasites in goats of this region are resistant to albendazole and levamisole. The P. rufescens showed high resistance to these drugs. Totally, it can be concluded that the level of drug resistance varies in different parts of the world; but, the frequencies of drug resistance in different parts of the world are not the same, requiring more studies.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"181-186"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug resistance and virulence-associated genes screening in Salmonella enterica isolated from Caspian pony, Iran. 伊朗里海小马分离的肠炎沙门氏菌的耐药性和毒力相关基因筛选。

IF 0.8 4区农林科学

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-09-15 DOI: 10.30466/vrf.2024.2014684.4051

Jamaladdin Eydi, Amir Tukmechi

{"title":"Drug resistance and virulence-associated genes screening in Salmonella enterica isolated from Caspian pony, Iran.","authors":"Jamaladdin Eydi, Amir Tukmechi","doi":"10.30466/vrf.2024.2014684.4051","DOIUrl":"10.30466/vrf.2024.2014684.4051","url":null,"abstract":"The most serious problem in public health is salmonellosis, a common disease in horse. The aim of this study was to investigate the shedding of Salmonella serotypes in healthy Caspian pony. We examined 143 pony's fecal samples collected from the north of Iran belonging to different ages and sexes. Samples were cultured, then identification of isolates were performed by common bacteriological methods and polymerase chain reaction (PCR). The PCR was also used to explore the presence of fimA and salmonella secreted effector L (SseL) genes as virulence factors in the isolates and all were assigned to antibiotic susceptibility test via disc diffusion method. Results showed two fecal samples (1.39%) contaminated with Salmonella and further examination demonstrated the isolates belonging to S. enterica serotype typhimurium. Both serotypes were isolated from female and ˂6 years of age group of ponies and we detected fimA and SseL genes in the isolates. Observing multiple drug resistance and virulence genes in isolates is of utmost importance from both clinical and public health perspectives. It is highly likely that we face instances of salmonellosis in animals or humans that lead to severe infections and fail to respond to treatment in future. This study revealed that the occurrence of Salmonella was low in ponies, however, regarding the presence of virulence factors with multidrug resistant trend in this zoonotic bacterium, establishment of good hygienic measurement to prevent the transmission of bacteria between animal and human is necessary.","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 9","pages":"481-486"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0