Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-10-15DOI: 10.30466/vrf.2024.2010315.3998
Shaaban Tayar Sadiq, Alaa Hussain Ali Al-Hamdani, Zanan Mohamed-Ameen Taha
{"title":"Clonal dispersion and pathogenic potential of multidrug-resistant <i>Aeromonas</i> spp. isolated from <i>Oncorhynchus mykiss</i> with hemorrhagic septicemia.","authors":"Shaaban Tayar Sadiq, Alaa Hussain Ali Al-Hamdani, Zanan Mohamed-Ameen Taha","doi":"10.30466/vrf.2024.2010315.3998","DOIUrl":"10.30466/vrf.2024.2010315.3998","url":null,"abstract":"<p><p>This study was important to improve proper biosecurity measures and controlling the spread of <i>Aeromonas</i> to prevent future outbreaks. This research sought to determine whether virulent <i>Aeromans</i> species were present in morbid rainbow trout, their resistance and their genetic relatedness. A total number of 542 tissue lesion specimens were collected from gill, liver, heart and kidneys in morbid domesticated fish in Duhok province, Iraq. The <i>gyrB</i> DNA sequence analysis was used to determine the species classification. Drug susceptibility testing was conducted for all isolated strains using disc diffusion technique. The genotyping analysis was carried out using enterobacterial repetitive intergenic consensus-polymerase chain reaction. Thirty-four isolates were found and they were classified into three species (<i>Aeromonas veronii, Aeromonas sorbia,</i> and <i>Aeromonas allosaccharophila)</i>, where <i>A. veronii</i> stand as one of the most prevalent species. The most frequently affected organ by <i>Aeromonas</i> was the gills among four different organs. The detection frequencies of the virulence genes <i>aerolysin,</i> <i>outer membrane protein, glycerophospholipid-cholesterol acyltransferase, elastase, flagella,</i> <i>serine protease, cytotonic heat-</i>labile<i>,</i> and <i>hemolysin</i> were 100%, 100%, 79.41%, 64.70%, 76.47%, 67.64%, 70.58%, and 41.17, respectively. None of the strains possessed all of the virulence markers. All isolates were completely resistant to ceftazidime, amoxicillin and doxycycline. All isolates were found to be multi-drug-resistant. Regardless of the nearest geographic source area of samples and the same <i>Aeromonas</i> species, there was a high genetic diversity. The results of this study could help farmers and researchers make informed decisions about measures of biosecurity and proper therapeutic drugs to apply to prevent current outbreaks and prevent them from recurring again.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 10","pages":"529-536"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-12-15DOI: 10.30466/vrf.2024.2024815.4202
Zohreh Mostahsan, Saeed Azizi, Ali Soleimanzadeh, Ali Shalizar-Jalali
{"title":"Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.","authors":"Zohreh Mostahsan, Saeed Azizi, Ali Soleimanzadeh, Ali Shalizar-Jalali","doi":"10.30466/vrf.2024.2024815.4202","DOIUrl":"https://doi.org/10.30466/vrf.2024.2024815.4202","url":null,"abstract":"<p><p>Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of unscavenged reactive oxygen species. This study aimed to investigate the effects of intra-peritoneal administration of Mito-TEMPO (MT) on I/R injury in testicular torsion/detorsion (T/D) in mice. Forty-two male mice were divided into seven groups including 1 control and 6 treatment groups (360° T/D, 720° T/D, 360° T/D + 0.70 mg kg<sup>-1</sup> MT, 360° T/D + 1.00 mg kg<sup>-1</sup> MT, 720° T/D + 0.70 mg kg<sup>-1</sup> MT, and 720° T/D + 1.00 mg kg<sup>-1</sup> MT). After inducing 360° and 720° clockwise testicular torsions for 2 hr, sperm parameters, apoptosis-related genes expression, and <i>in vivo</i> fertility index were evaluated. The results showed that 720° T/D can lead to increased abnormal sperm morphology, sperm DNA damage, and <i>Bax</i> expression, while the <i>Bcl-2</i> expression was reduced compared to the other groups. In addition, it also had negative effects on sperm total and progressive motilities as well as viability and plasma membrane functionality (PMF). The results also showed that administration of MT to T/D mice can result in a reduction in abnormal sperm morphology, DNA damage, and <i>Bax</i> expression. It could also increase sperm total and progressive motilities, viability and PMF, <i>Bcl-2</i> expression, and <i>in vivo</i> fertility index. Based on our results, it is concluded that MT, when administered after spermatic cord torsion in mice, provides significant protection against acute testicular T/D injury.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"665-672"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-12-15DOI: 10.30466/vrf.2024.2023686.4186
Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad
{"title":"Viability of guinea pig (<i>Cavia porcellus</i>) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C.","authors":"Mozhdeh Heydari, Alaleh Rakhshanpour, Ramin Mazaheri Khameneh, Ali Soleimanzadeh Azad","doi":"10.30466/vrf.2024.2023686.4186","DOIUrl":"https://doi.org/10.30466/vrf.2024.2023686.4186","url":null,"abstract":"<p><p>The cooling procedure markedly diminishes the quality of guinea pig (<i>Cavia porcellus</i>) sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions. This study aimed to assess the viability of guinea pig spermatozoa diluted in Tris buffer-based extenders during storage at 5.00 ˚C. Sperm collection was carried out through castration of the animals. For this study, 10 adult male guinea pigs were utilized, being divided into four groups including phosphate-buffered saline (PBS), human tubal fluid (HTF), Tris-citric-glucose (TCG), and Tris-fructose-yolk (TFY) cultures. Evaluations including sperm motility, morphology, plasma membrane integrity, viability, and total count were conducted at 0, 24, and 48 hr after sampling. The results obtained indicated that at the 24-hr and 48-hr marks of the experiment, both overall and progressive motility percentages, viability, plasma membrane integrity, and morphology of sperms in the PBS and HTF cultures exhibited a significant increase in comparison with the TCG and TFY cultures. Consequently, it can be inferred that PBS and HTF cultures are highly effective in preserving the quality of guinea pig spermatozoa.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 12","pages":"691-696"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-01-15DOI: 10.30466/vrf.2023.2004451.3910
Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei
{"title":"Comparison of the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses.","authors":"Pegah Parhizkar, Rahim Mohammadi, Seyed Mohammad Hashemi-Asl, Ghader Jalilzadeh-Amin, Farshid Sarrafzadeh-Rezaei","doi":"10.30466/vrf.2023.2004451.3910","DOIUrl":"10.30466/vrf.2023.2004451.3910","url":null,"abstract":"<p><p>The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg<sup>-1</sup>) in combination with acepromazine maleate (0.05 mg kg<sup>-1</sup>) intravenously (IV). In treatment II (T2), the animals received intra-gastric administration of clonidine (0.002 mg kg<sup>-1</sup>) followed by acepromazine (0.05 mg kg<sup>-1</sup>; IV) after 60 min. Head height above the ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 min before and 5, 15, 30, 60, and 90 min after drug administration. In T2, recordings were made 5 min before clonidine, 55 min after clonidine administration, and then 5, 15, 30, 60, and 90 min after acepromazine injection. Analyses of the data showed there were not significant differences regarding HHAG and echocardiographic indices between two treatments. For sedation of healthy horses, it was concluded that intra-gastric administration of clonidine and IV administration of acepromazine showed similar sedative and cardiovascular effects compared to IV acepromazine-xylazine administration.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 1","pages":"21-26"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10921139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-08-15DOI: 10.30466/vrf.2024.2015987.4069
Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad
{"title":"Effect of curcumin on formalin-induced muscle pain in male rats: role of local cyclooxygenase system.","authors":"Nabat Naqshbandi, Esmaeal Tamaddonfard, Amir Erfanparast, Farhad Soltanalinejad-Taghiabad","doi":"10.30466/vrf.2024.2015987.4069","DOIUrl":"https://doi.org/10.30466/vrf.2024.2015987.4069","url":null,"abstract":"<p><p>Investigating the mechanisms responsible for pain processing of natural and synthetic chemical compounds is necessary to optimize pain management. Curcumin (Cur), the active ingredient of turmeric, exhibits potent analgesic and anti-inflammatory properties by employing multiple mechanisms at the local peripheral, spinal and supra-spinal levels. This study was aimed to investigate the effect of oral administration of Cur on muscle pain induced by intramuscular (IM) injection of formalin. To explore the possible local mechanisms, a cyclooxygenase (COX) inhibitor, diclofenac (Dic) and a COX product, prostaglandin E<sub>2</sub> (PGE<sub>2</sub>), were applied. The IM injection of formalin (25.00 µL, 2.50%) into the gastrocnemius muscle induced two distinct phases of hind leg flinching. A short-lasting (10 min) hind leg lifting was observed following IM injection of PGE<sub>2</sub> (2 µg kg<sup>-1</sup>, 25.00 µL). Oral administration of Cur (25.00 and 100 mg kg<sup>-1</sup>) and IM injection of 40.00 µg kg<sup>-1</sup> Dic attenuated formalin and PGE2 induced nociceptive behaviors. Contra-lateral IM injection of Dic did not change muscle pain induced by ipsilateral IM injection of formalin and PGE<sub>2</sub>. The second phase of formalin induced flinching as well as PGE<sub>2</sub> evoked lifting were more suppressed when 40.00 µg kg<sup>-1</sup> Dic and 100 mg kg<sup>-1</sup> Cur were used together. Locomotor activity was not changed by the above-mentioned treatments. It was concluded that the reducing effect of muscle pain of Cur might be related to the local inhibition of COX.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"411-416"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-08-15DOI: 10.30466/vrf.2024.2014818.4056
Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati
{"title":"Molecular detection of the <i>beta-lactamase Cefotaxime</i> gene in <i>Escherichia coli</i> from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island.","authors":"Kholik Kholik, Pudji Srianto, Aulanni'am Aulanni'am, Sri Pantja Madyawati","doi":"10.30466/vrf.2024.2014818.4056","DOIUrl":"https://doi.org/10.30466/vrf.2024.2014818.4056","url":null,"abstract":"<p><p>This study aims to detect <i>Escherichia coli</i> which encodes <i>beta-lactamase Cefotaxime</i> <i>(blaCTX-M)</i> gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of <i>E. coli</i> was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of <i>E. coli</i> which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the <i>blaCTX-M</i> gene of <i>E. coli</i> and sequences of the <i>blaCTX-M</i> gene were phylogenetically analyzed. The research results obtained three <i>E. coli</i> isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of <i>E. coli</i> was resistant to penicillin G and oxytetracycline. 66.66% of <i>E. coli</i> was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of <i>E. coli</i> was resistant to tetracycline. <i>Escherichia coli</i> isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of <i>E. coli</i> detection using PCR showed that three <i>E. coli</i> isolates encoded the <i>blaCTX-M</i> gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that <i>E. coli</i> from the reproductive tract of Bali cattle was related to <i>E. coli</i> that encoded <i>blaCTX-M-</i>14 isolated from humans.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 8","pages":"397-402"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-11-15DOI: 10.30466/vrf.2024.2024154.4196
Farid Saghghaei, Ali Rassouli, Goudarz Sadeghi-Hashjin, Farhang Sasani, Mohammad Kazem Koohi
{"title":"Protective effects of <i>Nigella sativa</i> oil, thymoquinone and dexamethasone on bleomycin-induced lung fibrosis in rats.","authors":"Farid Saghghaei, Ali Rassouli, Goudarz Sadeghi-Hashjin, Farhang Sasani, Mohammad Kazem Koohi","doi":"10.30466/vrf.2024.2024154.4196","DOIUrl":"10.30466/vrf.2024.2024154.4196","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a chronic interstitial lung disease with a progressive damage to the air sacs and deposition of collagen fibers in the lung tissue. The study aimed to explore the effects of <i>Nigella sativa</i> oil (NSO) or thymoquinone (TQ), alone or in combination with dexamethasone (DEX), on the development of bleomycin (BLM)-induced PF. Forty-two male rats were divided into seven groups: Control (CTRL); BLM, received a single dose of BLM on day 0, intratracheally; all remaining groups received BLM, as well. DEX, received DEX daily, intraperitoneally, 1 day before BLM and continued for 14 days; NSO and TQ groups, received daily NSO and TQ, respectively, 7 days before BLM and continued for 35 days; DEX + TQ, received both DEX and TQ; DEX + NSO, received both DEX and NSO. At the end, lung tissues were used for histopathological and biochemical analyses. BLM significantly increased the severity of fibrosis and inflammation compared to the CTRL. Bleomycin also significantly increased the amount of hydroxyproline, however, decreased most antioxidant enzymes in the lung tissue compared to the other groups. Group TQ + DEX significantly reduced the severity of BLM-induced PF as well as alterations in biochemical parameters, lung weight and O2 saturation. <i>Nigella sativa</i> oil slightly reduced BLM-induced PF, however, it caused non-significant hyperemia in lung tissue. Thymoquinone potentiated the effects of DEX on most biochemical and pathological alterations of BLM-induced lung injury much better than NSO. More studies are needed to support the use of NSO and TQ as potential protective agents against PF.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"613-620"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MicroRNA-142-3p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression.","authors":"Masoumeh Moradi Ozarlou, Razeieh Dehghan, Behzad Mansoori, Behzad Baradaran","doi":"10.30466/vrf.2024.2022533.4165","DOIUrl":"10.30466/vrf.2024.2022533.4165","url":null,"abstract":"<p><p>Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-142-3p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-142-3p was initially assessed in MDA-MB-468 cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-142-3p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-142-3p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX2, Octamer 4, HMGA2, Kruppel-like factor 4, and Bach-1. Additionally, the combination of miRNA-142-3p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G<sup>1</sup> phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-142-3p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-142-3p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 11","pages":"629-643"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of <i>Brucella</i> spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023.","authors":"Saeed Alamian, Karim Amiry, Afshar Etemadi, Maryam Dadar","doi":"10.30466/vrf.2024.2012972.4028","DOIUrl":"10.30466/vrf.2024.2012972.4028","url":null,"abstract":"<p><p>Bovine brucellosis, an infectious disease transmitted by <i>Brucella melitensis</i> and <i>Brucella abortus</i>, presents a significant zoonotic risk for agricultural economics and animal health. The primary objective of this study was to present a comprehensive understanding of the prevalence and features of <i>Brucella</i> strains within the industrial dairy farming sector in Iran. Rose Bengal plate test, standard agglutination test, and indirect enzyme linked immunosorbent assay tests were used to confirm all seropositive animals. A total number of 1,311 bovine samples from seropositive animals including were collected from 224 farms in 21 provinces of different regions of Iran and examined. The discovered <i>Brucella</i> isolates were phenotyped and molecularly characterized. The isolates were all <i>B. abortus</i> or <i>B. melitensis</i>. Bacteria analysis revealed that 70.53% of seropositive farms were tested positive for <i>Brucella</i> strains, predominantly <i>B. melitensis</i> biovar 1 (43.42%) and <i>B. abortus</i> biovar 3 (27.11%). Geographical distribution revealed that <i>B. melitensis</i> biovar 1 was the most common in dairy cow farms (16 provinces), followed by <i>B. abortus</i> biovar 3 (six provinces). Also, the prevalence of <i>B. melitensis</i> biovar 2, <i>B. melitensis</i> biovar 3, <i>B. abortus</i> biovar 1, <i>B. abortus</i> biovar 2 and RB51 vaccine were restricted to certain provinces. AMOS (<i>abortus melitensis ovis suis</i>)-polymerase chain reaction and Bruce-ladder PCR confirmed species identification. These results highlighted the complexity of bovine brucellosis in Iran and illustrated that <i>B. melitensis</i> was spread from small ruminants to cattle. This study provided important epidemiological insights for targeting future brucellosis control programs in the Iranian dairy farms.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"195-202"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinary Research ForumPub Date : 2024-01-01Epub Date: 2024-04-15DOI: 10.30466/vrf.2024.2011964.4014
Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei
{"title":"Generalized megaesophagus associated with Schmidt-like syndrome in a dog.","authors":"Reza Azargoun, Seyed Mohammad Hashemi-Asl, Siamak Asri-Rezaei","doi":"10.30466/vrf.2024.2011964.4014","DOIUrl":"10.30466/vrf.2024.2011964.4014","url":null,"abstract":"<p><p>A 2-year-old intact male Asian Shepherd dog was referred with a history of chronic regurgitation along with normal appetite and diagnosis of megaesophagus on plain radiography. Clinical examination revealed normothermia, normocardia, normopnea, low body condition score and poor hair coat. The most important laboratory findings include anemia, azotemia, hyperlipidemia, increased thyroid stimulating hormone, decreased thyroxine and hypocortisolemia, as well as a marked increase in acetylcholine receptor antibody concentration. Based on the results, in addition to primary hypothyroidism and primary hypoadrenocorticism, myasthenia gravis was also diagnosed as an underlying cause of megaesophagus. Following nursing care and preferred treatment of each disease, the megaesophagus was resolved in the next visit. This clinical report describes for the first time, to the authors' knowledge, a dog with a rare type of autoimmune polyglandular syndrome (APS) known in human medicine as a Schmidt's syndrome. We want to emphasize the importance of clinicians' awareness regarding the possibility of APS to identify different diseases caused by it in order to achieve successful treatment.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":"15 4","pages":"215-218"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}