Materials Today Bio最新文献

{"title":"A novel nanomedicine integrating ferroptosis and photothermal therapy, well-suitable for PD-L1-mediated immune checkpoint blockade","authors":"Yujun Bao ,&nbsp;Guanghao Li ,&nbsp;Siqi Li ,&nbsp;Haishui Zhou ,&nbsp;Ziqing Yang ,&nbsp;Zhiqiang Wang ,&nbsp;Rui Yan ,&nbsp;Changhong Guo ,&nbsp;Yingxue Jin","doi":"10.1016/j.mtbio.2024.101346","DOIUrl":"10.1016/j.mtbio.2024.101346","url":null,"abstract":"<div><div>Immunotherapy based on immune checkpoint blockade has emerged as a promising treatment strategy; however, the therapeutic efficacy is limited by the immunosuppressive microenvironment. Here, we developed a novel immune-activated nanoparticle (Fc-SS-Fe/Cu) to address the issue of insufficient immune infiltration. Specifically, the structure of Fc-SS-Fe/Cu collapsed in response to the tumor microenvironment, the ferrocene and disulfide bonds and the released Fe/Cu ions can effectively generate ·OH and deplete GSH to increase oxidative stress, thereby inducing ferroptosis. Withal, the positive feedback mechanisms of \"laser-triggered mild-temperature photothermal therapy (PTT), PTT accelerated ferroptosis and LPO accumulation, LPO mediated HSPs down-regulated to promote PTT,\" effectively triggers immunogenic cell death (ICD) in tumor cells, significantly enhancing their immunogenicity. Moreover, the O₂-generating ability induced by Fc-SS-Fe/Cu could reverse the hypoxic tumor microenvironment, and importantly, the expression of PD-L1 on tumor cell surfaces could be effectively downregulated by inhibiting the HIF-1<em>α</em> pathways, thereby augmenting the effect of anti-PD-L1 (<em>α</em>PD-L1) therapy. Therefore, this study provides valuable strategies into enhancing PD-L1-mediated ICB therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101346"},"PeriodicalIF":8.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nickel–titanium alloy porous scaffolds based on a dominant cellular structure manufactured by laser powder bed fusion have satisfactory osteogenic efficacy","authors":"Jiaming Lin ,&nbsp;An Yan ,&nbsp;Anfei Huang ,&nbsp;Qinglian Tang ,&nbsp;Jinchang Lu ,&nbsp;Huaiyuan Xu ,&nbsp;Yufeng Huang ,&nbsp;Tianqi Luo ,&nbsp;Zhihao Chen ,&nbsp;Anyu Zeng ,&nbsp;Xiaojun Zhu ,&nbsp;Chao Yang ,&nbsp;Jin Wang","doi":"10.1016/j.mtbio.2024.101344","DOIUrl":"10.1016/j.mtbio.2024.101344","url":null,"abstract":"<div><div>Nickel–titanium (NiTi) alloy is a widely utilized medical shape memory alloy (SMA) known for its excellent shape memory effect and superelasticity. Here, laser powder bed fusion (LPBF) technology was employed to fabricate a porous NiTi alloy scaffold featuring a topologically optimized dominant cellular structure that demonstrates favorable physical and superior biological properties. Utilizing a porous structure topology optimization method informed by the stress state of human bones, two types of cellular structures—compression and torsion—were designed, and porous scaffolds were produced via LPBF. The physical properties of the porous NiTi alloy scaffolds were evaluated to confirm their biocompatibility, while their osteogenic efficacy was investigated through both in vivo and in vitro experiments, with comparisons made against a traditional octahedral unit cell structure. NiTi alloy porous scaffolds can be nearly net-shaped via LPBF and exhibit favorable physical properties, including a low elastic modulus, high hydrophilicity, a specific linear expansion rate, as well as superelastic and shape memory effects. These scaffolds demonstrate excellent biocompatibility, support in vitro osteogenesis, and possess significant in vivo bone ingrowth capabilities. When compared to titanium alloys, NiTi alloys show comparable osteogenic properties in vitro but superior bone ingrowth properties in vivo. Additionally, among octahedral-type, torsion-type, and topologically optimized compression-type porous scaffolds, the latter demonstrates enhanced bone ingrowth properties. LPBF technology is effective for manufacturing porous NiTi alloy scaffolds with fine pore structures and excellent mechanical properties. The scaffolds based on topologically optimized dominant cellular structures facilitate satisfactory and efficient bone formation.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101344"},"PeriodicalIF":8.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative medicine: Hydrogels and mesoporous silica nanoparticles","authors":"Jesús L. Pablos ,&nbsp;Daniel Lozano ,&nbsp;Miguel Manzano ,&nbsp;María Vallet-Regí","doi":"10.1016/j.mtbio.2024.101342","DOIUrl":"10.1016/j.mtbio.2024.101342","url":null,"abstract":"<div><div>Hydrogels, that are crosslinked polymer networks, can absorb huge quantities of water and/or biological fluids. Their physical properties, such as elasticity and soft tissue, together with their biocompatibility and biodegradability, closely resemble living tissues. The versatility of hydrogels has fuelled their application in various fields, such as agriculture, biomaterials, the food industry, drug delivery, tissue engineering, and regenerative medicine. Their combination with nanoparticles, specifically with Mesoporous Silica Nanoparticles (MSNs), have elevated these composites to the next level, since MSNs could improve the hydrogel mechanical properties, their ability to encapsulate and controlled release great amounts of different therapeutic agents, and their responsiveness to a variety of external and internal stimuli. In this review, the main features of both MSNs and hydrogels are introduced, followed by the discussion of different hydrogels-MSNs structures and an overview of their use in different applications, such as drug delivery technologies and tissue engineering.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101342"},"PeriodicalIF":8.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercially available bioinks and state-of-the-art lab-made formulations for bone tissue engineering: A comprehensive review","authors":"Elena Alina Chiticaru ,&nbsp;Mariana Ioniță","doi":"10.1016/j.mtbio.2024.101341","DOIUrl":"10.1016/j.mtbio.2024.101341","url":null,"abstract":"<div><div>Bioprinting and bioinks are two of the game changers in bone tissue engineering. This review presents different bioprinting technologies including extrusion-based, inkjet-based, laser-assisted, light-based, and hybrid technologies with their own strengths and weaknesses. This review will aid researchers in the selection and assessment of the bioink; the discussion ranges from commercially available bioinks to custom lab-made formulations mainly based on natural polymers, such as agarose, alginate, gelatin, collagen, and chitosan, designed for bone tissue engineering. The review is centered on technological advancements and increasing clinical demand within the rapidly growing bioprinting market. From this point of view, 4D, 5D, and 6D printing technologies promise a future where unprecedented levels of innovation will be involved in fabrication processes leading to more dynamic multifunctionalities of bioprinted constructs. Further advances in bioprinting technology, such as hybrid bioprinting methods are covered, with the promise to meet personalized medicine goals while advancing patient outcomes for bone tissues engineering applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101341"},"PeriodicalIF":8.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable microgels containing genetically engineered bacteria for colon cancer therapy through programmed Chemokine expression","authors":"Yazhou Chen ,&nbsp;Kehan Cai ,&nbsp;Hui Zhao ,&nbsp;Wenshuai Li ,&nbsp;Xiaofang Gao ,&nbsp;Yinzheng Fu ,&nbsp;Kyubae Lee ,&nbsp;SiTian Li ,&nbsp;Shengjie Yao ,&nbsp;Tao Chen","doi":"10.1016/j.mtbio.2024.101337","DOIUrl":"10.1016/j.mtbio.2024.101337","url":null,"abstract":"<div><div>Chemokines are emerging as important targets for cancer immunotherapy due to their role in regulating immune cell migration and activation within the tumor microenvironment. Effective delivery and sustained presence of chemokines at the tumor site is essential for recruiting and activating immune cells to exert anti-tumor effects. In this study, we report a genetically engineered bacterial cell factory designed for the continuous production of chemokine CCL21 in a controlled manner. To decrease the formation of infusion bodies (IBs) in bacteria, we used thioredoxin (Trx) as the fusion partner and cloned at N-terminal of the target protein. The commonly used promoters, pT7-LacO, pBV220, and pDawn, were employed to explore the influence of various inducers on the expression of CCL21 in bacteria. The engineered bacteria were finally encapsulated within spherical gelatin methacryloyl (GelMA) microgels, which not only maintained bacterial viability but also prolonged their retention in the intestines of mice. As a result, the sustained presence and localized production of CCL21 led to effective suppression of tumor growth.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101337"},"PeriodicalIF":8.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-water retention, self-healing hydrogel thyroid model for surgical training","authors":"Liang Ma ,&nbsp;Zhihao Zhu ,&nbsp;Shijie Yu ,&nbsp;Sidney Moses Amadi ,&nbsp;Fei Zhao ,&nbsp;Jing Zhang ,&nbsp;Zhifei Wang","doi":"10.1016/j.mtbio.2024.101334","DOIUrl":"10.1016/j.mtbio.2024.101334","url":null,"abstract":"<div><div>The evaluation of thyroid lesions through Fine-Needle Aspiration Biopsy (FNAB) is a common procedure that requires advanced hand manipulation skills. Conventional training models for this procedure lack essential features such as tactile sensation and the ability to repeat punctures similar to those of real organs. To improve the quality of training, we have developed a hydrogel thyroid model that possesses features such as high-water retention and self-healing properties. This model consists of polyvinyl alcohol (PVA), polyacrylic acid (PAA), and trehalose that enhance water retention. By utilizing indirect printing technology, this hydrogel-based thyroid model closely resembles those of porcine thyroid tissue in terms of compression modulus and friction coefficient, exhibiting exceptional conformability, flexibility, and a water retention rate of 94.7 % at 6 h. It also displays a thrust force range of 0–0.98 N during simulated puncture, closely approximating real FNAB operations. This model shows evidence that it effectively simulates thyroid tissue and can be utilized for repetitive FNAB training to enhance the proficiency of medical personnel. Our study focuses on introducing new possibilities for developing advanced materials training models to be utilized in the medical field.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101334"},"PeriodicalIF":8.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional hydrogels loaded with tellurium nanozyme for spinal cord injury repair","authors":"Jian Meng ,&nbsp;Jingjing Sun ,&nbsp;Jiali Kang ,&nbsp;Shilei Ren ,&nbsp;Miaomiao Xu ,&nbsp;Runzhi Li ,&nbsp;Xuhui Zhao ,&nbsp;Yitong Yuan ,&nbsp;Lei Xin ,&nbsp;Ruiping Zhang","doi":"10.1016/j.mtbio.2024.101339","DOIUrl":"10.1016/j.mtbio.2024.101339","url":null,"abstract":"<div><div>Spinal cord injury (SCI) results in severe neurological deficits due to disrupted neural pathways. While the spinal cord possesses limited self-repair capabilities, recent advancements in hydrogel-based therapies have shown promise. Polyphenol-based hydrogels, known for their neuroprotective properties, offer a suitable microenvironment for neural regeneration. In this study, a novel poly(lipoic acid)/poly(dopamine) adhesive hydrogel was developed as a versatile platform for delivering therapeutic agents. This hydrogel was loaded with methylcobalamin, a neurotrophic factor, and tellurium nanoenzymes, potent antioxidants. The nanoenzymes effectively mitigated oxidative stress and inflammation, while methylcobalamin promoted nerve regeneration. The combined therapeutic effects of the nanoenzymatic hydrogel demonstrated significant efficacy in repairing spinal cord injuries, highlighting its potential as a promising strategy for treating this debilitating condition.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101339"},"PeriodicalIF":8.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing hydrogen sulfide in injectable hydrogels that guide the immune response and osteoclastogenesis balance for osteoporosis treatment","authors":"Lianghua Jiang ,&nbsp;Yubin Wu ,&nbsp;Zonghan Xu ,&nbsp;Mingzhuang Hou ,&nbsp;Shayang Chen ,&nbsp;Chao Cheng ,&nbsp;Dan Hu ,&nbsp;Daming Lu ,&nbsp;Xuesong Zhu ,&nbsp;Chong Li","doi":"10.1016/j.mtbio.2024.101338","DOIUrl":"10.1016/j.mtbio.2024.101338","url":null,"abstract":"<div><div>Elevated levels of oxidative stress, inflammation, and a dysregulated osteoclastogenesis balance frequently characterize the microenvironment of osteoporosis, which impedes the processes of healing and repair. Existing treatment approaches are limited in scope and rely primarily on factors and drugs. An injectable hydrogel designed for the ROS-responsive release of H₂S gas is presented in this study. The first network of the hydrogel comprises sodium alginate (SA-SATO) chelated with S-aroylthiooxime (SATO) and an H₂S-generating group, while the second network is composed of photocrosslinkable PEGDA. Through the integration of Cys-releasing microspheres that are reactive with ROS, a composite hydrogel was developed that exhibited advantageous mechanical characteristics and biosafety. The composite hydrogel effectively promoted osteogenic differentiation of mesenchymal stem cells, modulated macrophage polarization, decreased inflammatory responses, and halted cell apoptosis, as evidenced by in vitro experiments. Additionally, it released H₂S gas and mitigated excess ROS in cells. The efficacy of the composite hydrogel in promoting bone defect repair and regeneration in an osteoporotic model was further validated by in vivo findings. In summary, the composite hydrogel exhibits potential as a viable approach to address osteoporotic bone defects by harmonizing osteogenesis and osteoclast activity, modulating the microenvironment of bone injuries, and reducing inflammation. Consequently, it presents a viable strategy for the efficient repair of bone defects.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101338"},"PeriodicalIF":8.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive hydrogel synergizes neuroprotection, macrophage polarization, and angiogenesis to improve repair of traumatic brain injury","authors":"Yifan Hao ,&nbsp;Longbao Feng ,&nbsp;Huiling Liu ,&nbsp;Liming Zhou ,&nbsp;Xiang Yu ,&nbsp;Xinyue He ,&nbsp;Huan Cheng ,&nbsp;Long Jin ,&nbsp;Changyong Wang ,&nbsp;Rui Guo","doi":"10.1016/j.mtbio.2024.101335","DOIUrl":"10.1016/j.mtbio.2024.101335","url":null,"abstract":"<div><div>Traumatic brain injury (TBI) can lead to severe neurotrauma, leading to long-term cognitive decline and even death. Massive neuronal loss and excessive neuroinflammation are critical issues in the treatment of secondary TBI. To tackle these challenges, we developed a GelMA and CSMA hydrogel loaded with Erythropoietin (EPO) and Interleukin-4 (IL-4), named GC/I/E. By directly loading the hydrogel with EPO, rapid neuroprotection and angiogenesis were achieved. Meanwhile, by loading Mesoporous silica nanoparticles (MSNs) with IL-4 (MSN@IL-4), sustained inflammation modulation during inflammation was attained. <em>In vitro</em> experiments demonstrated that GC/I/E hydrogel were biocompatible and could provide neuroprotection for HT22 cells in H₂O₂ environment, regulate RAW264.7 polarization from M1 to M2 phenotype and promote HUVEC angiogenesis. <em>In vivo</em> experiments demonstrated that GC/I/E hydrogel reduced brain edema and Nissl body damage, inhibited inflammatory expression of G3-FFAP and neural scarring, improved microvascular and vascular function reconstruction, and facilitated neuronal and synaptogenesis, ultimately improving neurofunctional recovery in TBI. RNA sequencing results demonstrated that GC/I/E hydrogel treatment significantly correlated with the regulation of genes such as apoptosis, inflammation regulation, and neural regeneration. This bioactive hydrogel with neuroprotection, inflammation modulation and promotion of angiogenesis has great potential for TBI treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101335"},"PeriodicalIF":8.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of vascular grafts based on tissue-engineered scaffolds","authors":"Zhongliang Lang ,&nbsp;Tianao Chen ,&nbsp;Shilu Zhu ,&nbsp;Xizhi Wu ,&nbsp;Yongqi Wu ,&nbsp;Xiaoping Miao ,&nbsp;Qiang Wang ,&nbsp;Liping Zhao ,&nbsp;Zhiqiang Zhu ,&nbsp;Ronald X. Xu","doi":"10.1016/j.mtbio.2024.101336","DOIUrl":"10.1016/j.mtbio.2024.101336","url":null,"abstract":"<div><div>Cardiovascular disease (CVD) ranks among the leading causes of morbidity and mortality globally, primarily due to arterial occlusive disease. Vascular bypass remains the cornerstone of treatment; however, many patients lack suitable autologous vessels (e.g., saphenous vein) for grafting. Tissue-engineered vascular grafts (TEVGs) provide a viable alternative capable of integrating, remodeling, and repairing host vessels, responding to mechanical and biochemical stimuli. Currently, preparation methods for TEVGs are mainly categorized into scaffold-free and scaffold-based approaches. Scaffold-free methods exhibit comparatively weaker mechanical properties and limited research progress, whereas scaffold-based approaches show more promising applications due to their superior mechanical properties and biocompatibility. This review examines current research progress in materials, fabrication processes, functionalized modifications, cell implantation, and animal and clinical experiments for scaffold-based preparation of TEVGs. By exploring current challenges and future perspectives in this field, we expect to provide new insights into TEVGs development and expedite their clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101336"},"PeriodicalIF":8.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}