{"title":"活细菌负载抗菌水凝胶涂层纱布:多重耐药细菌感染的革命性解决方案","authors":"Minghui Xie , Yang Xue , Wantong Lin , Zibing Jiang , Yichun Chen , Yue Pei , Xu Wu , Xiubin Xu","doi":"10.1016/j.mtbio.2025.102337","DOIUrl":null,"url":null,"abstract":"<div><div>Chronic wounds infected by methicillin-resistant <em>Staphylococcus aureus</em> (<em>MRSA</em>) pose critical therapeutic challenges due to the prevalence of multi-drug-resistant (MDR) pathogens. To address this issue, a biocompatible live bacteria-loaded hydrogel-coated gauze (APAG) was developed by integrating an engineering <em>Pseudomonas</em> sp. SC11pLAFR-GFP with an alginate/ε-polylysine-polyacrylamide (Alg/ε-PL-PAAm) double-network hydrogel. The hierarchical hydrogel architecture, achieved through physical entrapment in the Alg/ε-PL core, ensures bacterial viability, while the chemically crosslinked PAAm outer layer regulates metabolite diffusion kinetics, synergistically enhancing therapeutic efficacy. The engineered bacteria hydrogel gauze (SC11@APAG) demonstrated sustained antimicrobial production for more than 48 h through the metabolic activity of the loaded <em>Pseudomonas</em> sp. SC11. This biohybrid not only effectively combats <em>MRSA</em> infection in mice wounds but also enhances wound healing. This “bacterial antagonism” strategy combines the native antimicrobial properties of <em>Pseudomonas</em> sp. with advanced material engineering, establishing a new paradigm for intelligent biomedical textiles in combating antimicrobial resistance.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102337"},"PeriodicalIF":10.2000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Living bacteria-loaded antibacterial hydrogel-coated gauze: A revolutionary solution for multi-drug-resistant bacterial infections\",\"authors\":\"Minghui Xie , Yang Xue , Wantong Lin , Zibing Jiang , Yichun Chen , Yue Pei , Xu Wu , Xiubin Xu\",\"doi\":\"10.1016/j.mtbio.2025.102337\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Chronic wounds infected by methicillin-resistant <em>Staphylococcus aureus</em> (<em>MRSA</em>) pose critical therapeutic challenges due to the prevalence of multi-drug-resistant (MDR) pathogens. To address this issue, a biocompatible live bacteria-loaded hydrogel-coated gauze (APAG) was developed by integrating an engineering <em>Pseudomonas</em> sp. SC11pLAFR-GFP with an alginate/ε-polylysine-polyacrylamide (Alg/ε-PL-PAAm) double-network hydrogel. The hierarchical hydrogel architecture, achieved through physical entrapment in the Alg/ε-PL core, ensures bacterial viability, while the chemically crosslinked PAAm outer layer regulates metabolite diffusion kinetics, synergistically enhancing therapeutic efficacy. The engineered bacteria hydrogel gauze (SC11@APAG) demonstrated sustained antimicrobial production for more than 48 h through the metabolic activity of the loaded <em>Pseudomonas</em> sp. SC11. This biohybrid not only effectively combats <em>MRSA</em> infection in mice wounds but also enhances wound healing. This “bacterial antagonism” strategy combines the native antimicrobial properties of <em>Pseudomonas</em> sp. with advanced material engineering, establishing a new paradigm for intelligent biomedical textiles in combating antimicrobial resistance.</div></div>\",\"PeriodicalId\":18310,\"journal\":{\"name\":\"Materials Today Bio\",\"volume\":\"35 \",\"pages\":\"Article 102337\"},\"PeriodicalIF\":10.2000,\"publicationDate\":\"2025-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materials Today Bio\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590006425009081\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Today Bio","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590006425009081","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Living bacteria-loaded antibacterial hydrogel-coated gauze: A revolutionary solution for multi-drug-resistant bacterial infections
Chronic wounds infected by methicillin-resistant Staphylococcus aureus (MRSA) pose critical therapeutic challenges due to the prevalence of multi-drug-resistant (MDR) pathogens. To address this issue, a biocompatible live bacteria-loaded hydrogel-coated gauze (APAG) was developed by integrating an engineering Pseudomonas sp. SC11pLAFR-GFP with an alginate/ε-polylysine-polyacrylamide (Alg/ε-PL-PAAm) double-network hydrogel. The hierarchical hydrogel architecture, achieved through physical entrapment in the Alg/ε-PL core, ensures bacterial viability, while the chemically crosslinked PAAm outer layer regulates metabolite diffusion kinetics, synergistically enhancing therapeutic efficacy. The engineered bacteria hydrogel gauze (SC11@APAG) demonstrated sustained antimicrobial production for more than 48 h through the metabolic activity of the loaded Pseudomonas sp. SC11. This biohybrid not only effectively combats MRSA infection in mice wounds but also enhances wound healing. This “bacterial antagonism” strategy combines the native antimicrobial properties of Pseudomonas sp. with advanced material engineering, establishing a new paradigm for intelligent biomedical textiles in combating antimicrobial resistance.
期刊介绍:
Materials Today Bio is a multidisciplinary journal that specializes in the intersection between biology and materials science, chemistry, physics, engineering, and medicine. It covers various aspects such as the design and assembly of new structures, their interaction with biological systems, functionalization, bioimaging, therapies, and diagnostics in healthcare. The journal aims to showcase the most significant advancements and discoveries in this field. As part of the Materials Today family, Materials Today Bio provides rigorous peer review, quick decision-making, and high visibility for authors. It is indexed in Scopus, PubMed Central, Emerging Sources, Citation Index (ESCI), and Directory of Open Access Journals (DOAJ).