电针华佗夹脊穴配合导电水凝胶可协同改善脊髓损伤后膀胱及运动功能

IF 10.2 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Qiang Zhang , Feng Wei , Lulu Zhang , Can Liang , Yan Zhuang , Ning Jiang , Wenjie Weng , Xusheng Qiu , Huiru Wang , Yixin Chen , Jianwu Dai , He Shen
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引用次数: 0

摘要

脊髓损伤损害了组织的电生理特性,破坏了电传递途径,最终导致运动功能障碍和神经源性膀胱损伤。因此,恢复损伤脊髓组织的正常电生理功能对脊髓损伤康复至关重要。电刺激(ES)通过神经元调节显示出治疗神经系统疾病的潜力,并与传统中医疗法(如靶向针灸)相结合,显示出更高的疗效。具体而言,针刺位于脊柱两侧的花托-夹脊穴,可发挥神经调节作用。在本研究中,我们开发了一种双模式的治疗方法,结合植入式仿生导电水凝胶治疗内源性ES和电针华脱夹脊穴治疗外源性ES,重建神经电微环境,协同促进脊髓损伤后电生理恢复。将碳纳米管掺入光交联明胶基质中制备的工程导电水凝胶具有促进内源电信号传播和增强外源ES效率的双重功能。这种联合治疗显著减轻炎症反应和神经胶质瘢痕形成,同时促进轴突再生和减轻脱髓鞘。功能评估显示,完全脊髓横断模型大鼠在运动恢复和神经源性膀胱控制方面均有显著改善。机制研究表明,Janus激酶信号转导和转录信号通路激活因子的下调是联合治疗抗炎作用的关键调节因子。总的来说,我们创新的内外ES整合策略通过同时增强神经再生和促进功能恢复,为脊髓损伤修复提供了一种有前景的转化方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Electroacupuncture at Huatuo-Jiaji acupoints combined with conductive hydrogel synergistically improves bladder and motor functions after spinal cord injury

Electroacupuncture at Huatuo-Jiaji acupoints combined with conductive hydrogel synergistically improves bladder and motor functions after spinal cord injury
Spinal cord injury (SCI) compromises the electrophysiological properties of tissues and disrupts the electrical transmission pathways, ultimately resulting in motor dysfunction and neurogenic bladder impairment. Therefore, restoration of normal electrophysiological functions in injured spinal cord tissues is crucial for SCI rehabilitation. Electrical stimulation (ES) exhibits therapeutic potential for neurological disorders via neuronal modulation, showing enhanced efficacy in combination with traditional Chinese medicine practices, such as targeted acupuncture. Specifically, acupuncture at Huatuo-Jiaji acupoints, located bilaterally along the vertebral column, exerts neurological regulatory effects. In this study, we developed a dual-modality therapeutic approach combining implantable biomimetic conductive hydrogels for internal ES with electroacupuncture at Huatuo-Jiaji acupoints for external ES to reconstruct the neural electrical microenvironment and synergistically enhance the electrophysiological recovery post-SCI. The engineered conductive hydrogels, fabricated by incorporating carbon nanotubes into photocrosslinkable gelatin matrices, showed dual functionality by facilitating endogenous electrical signal propagation and enhancing the exogenous ES efficacy. This combination therapy significantly attenuated the inflammatory responses and glial scar formation, concurrently promoting axonal regeneration and mitigating demyelination. Functional assessments revealed substantial improvements in both locomotor recovery and neurogenic bladder control in complete spinal cord transection model rats. Mechanistic investigations revealed the downregulation of the Janus kinase–signal transducer and activator of transcription signaling pathway as a key regulator of anti-inflammatory effects of the combination treatment. Overall, our innovative internal–external ES integration strategy offers a promising translational approach for SCI repair by simultaneously enhancing neural regeneration and facilitating functional restoration.
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来源期刊
CiteScore
8.30
自引率
4.90%
发文量
303
审稿时长
30 days
期刊介绍: Materials Today Bio is a multidisciplinary journal that specializes in the intersection between biology and materials science, chemistry, physics, engineering, and medicine. It covers various aspects such as the design and assembly of new structures, their interaction with biological systems, functionalization, bioimaging, therapies, and diagnostics in healthcare. The journal aims to showcase the most significant advancements and discoveries in this field. As part of the Materials Today family, Materials Today Bio provides rigorous peer review, quick decision-making, and high visibility for authors. It is indexed in Scopus, PubMed Central, Emerging Sources, Citation Index (ESCI), and Directory of Open Access Journals (DOAJ).
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