Materials Today Bio最新文献

{"title":"CD44-targeted virus-mimicking nanomedicine eliminates cancer stem cells and mitigates chemoresistance in head and neck squamous cell carcinoma","authors":"Yiwen Chen ,&nbsp;Zhen Qin ,&nbsp;Yujia Wang ,&nbsp;Baoxin Gu ,&nbsp;Jing Wang ,&nbsp;Yunfei Zheng ,&nbsp;Yuting Niu ,&nbsp;Lingfei Jia","doi":"10.1016/j.mtbio.2025.101721","DOIUrl":"10.1016/j.mtbio.2025.101721","url":null,"abstract":"<div><div>Cancer stem cells (CSCs) play critical roles in tumor growth, metastasis, and chemoresistance. Although several small-molecule inhibitors designed to inhibit CSCs have been investigated in clinical trials, their inadequate tumor targeting and potential off-target side effects have led to poor outcomes. A CD44-targeted virus-mimicking nanomedicine encapsulating the BMI1 inhibitor PTC209 (PTC209@VNP-HA) was designed to treat head and neck squamous cell carcinoma (HNSCC). We used a dendritic mesoporous silica nanoparticle (MSN) as the core for virus-mimicking nanoparticle (VNP) formation after adding shell particles to the MSN surface. The VNP surface was then modified with hyaluronic acid (HA), and PTC209 was adsorbed by mesopores to form PTC209@VNP-HA. In this system, HA is used to target CD44⁺ CSCs. The rough surface of VNP-HA provided better drug delivery efficiency than smooth nanoparticles modified with HA. VNP-HA enhanced the cancer inhibitory effect of PTC209 12-fold compared to the administration of free PTC209, leading to significantly higher bioavailability of PTC209. Both <em>in vitro</em> and <em>in vivo</em> assays showed that PTC209@VNP-HA inhibited cancer stemness, proliferation, and metastasis in HNSCC. Mechanistically, this inhibitory effect is closely associated with DNA damage/apoptosis signaling. Using a series of preclinical models in murine systems, we confirmed that PTC209@VNP-HA eliminated BMI1⁺ CSCs, and greatly inhibited the proliferation and metastasis of HNSCC when combined with cisplatin.This study investigated PTC209@VNP-HA as a novel and potentially transformative HNSCC treatment option that eliminates CSCs, prevents metastasis, and overcomes cisplatin resistance.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101721"},"PeriodicalIF":8.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo metabolic tagging and modulation of platelets","authors":"Dhyanesh Baskaran ,&nbsp;Yusheng Liu ,&nbsp;Jiadiao Zhou ,&nbsp;Yueji Wang ,&nbsp;Daniel Nguyen ,&nbsp;Hua Wang","doi":"10.1016/j.mtbio.2025.101719","DOIUrl":"10.1016/j.mtbio.2025.101719","url":null,"abstract":"<div><div>Platelets play a critical role in hemostasis at sites of injury and are capable of interacting with various types of cells in the bloodstream. The promise of utilizing platelets for diagnostic and therapeutic applications has motivated the development of facile strategies to functionalize platelets. However, platelets with a small size, lack of nucleus and efficient protein machinery, and low tolerance to chemicals and transfection agents have posed significant challenges for chemical or genetic engineering. Here, for the first time, we report successful metabolic glycan labeling of platelets to introduce chemical tags (e.g., azido groups) onto the membrane of platelets. We demonstrate that azido-sugars can metabolically label platelets in a concentration dependent manner, with cell-surface azido groups detectable at as early as 4 hours. The cell-surface azido groups enable the conjugation of various macromolecular cargos including proteins and polymers onto platelets via efficient click chemistry. Small-molecule drugs such as doxorubicin can also be conjugated onto azido-labeled platelets and become subsequently released to kill surrounding cancer cells, demonstrating the feasibility of utilizing platelets as a drug delivery vehicle. We further show that azido-sugars, upon intraperitoneal injection, can metabolically label platelets with azido groups <em>in vivo</em>, which persist for up to 4 days in mice (nearly the life-span of murine platelets). This <em>in vitro</em> and <em>in vivo</em> platelet labeling and targeting technology opens a new avenue to platelet-based diagnostics and therapeutics.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101719"},"PeriodicalIF":8.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the feasibility of CRISPRa approaches to enhance protein-based biomaterial expression in bacterial systems for more efficient production","authors":"Pablo Rodríguez-Alonso ,&nbsp;Viktoriya Chaskovska ,&nbsp;Desiré Venegas-Bustos ,&nbsp;Alba Herraiz ,&nbsp;Matilde Alonso ,&nbsp;Jose Carlos Rodríguez-Cabello","doi":"10.1016/j.mtbio.2025.101720","DOIUrl":"10.1016/j.mtbio.2025.101720","url":null,"abstract":"<div><div>Recombinant protein production is crucial for biomedical and industrial applications; however, achieving high yields for complex protein-like biomaterials such as elastin-like recombinamers (ELRs) remains challenging. ELRs, protein-based polymers derived from tropoelastin, emulate the mechanical and bioactive properties of natural tissues, making them valuable for numerous uses. Despite their promise, implementing a sophisticated molecular system for ELR production in <em>Escherichia coli</em> involves overcoming multiple hurdles, including metabolic bottlenecks and low yields. In this study, we employed a CRISPR activation (CRISPRa) system to enhance ELR expression in <em>E. coli</em>. Although further optimization is required to reach industrial-scale outputs, our findings establish a proof of concept for taking advantage of CRISPRa to boost recombinamers yields. Such improvements represent a crucial step toward scalable production, facilitating the commercial adoption of ELRs and, in general, recombinamers not only in biomedical applications but also in broader industries that stand to benefit from these versatile biomaterials.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101720"},"PeriodicalIF":8.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalin-functionalized ultrasmall nanoparticles modulate the complement pathway to inhibit microglial synaptic phagocytosis for reducing anesthetic neurotoxicity","authors":"Gang Wang ,&nbsp;Yaobao Han ,&nbsp;Ke Peng ,&nbsp;Zhilin Jiang ,&nbsp;Tingting Wang ,&nbsp;Qing Zheng ,&nbsp;Wenting Li ,&nbsp;Hanbing Xu ,&nbsp;Fuhai Ji ,&nbsp;Zhen Li","doi":"10.1016/j.mtbio.2025.101714","DOIUrl":"10.1016/j.mtbio.2025.101714","url":null,"abstract":"<div><div>Synaptic impairment is identified as a primary pathology in sevoflurane-induced neurotoxicity, contributing to neurobehavioral and neurodevelopmental deficits. Synaptic loss in neurons occurs through microglia-mediated synaptic phagocytosis <em>via</em> the complement pathway. Astragalin, a natural flavonoid compound, exhibits diverse bioactivities, such as anti-tumor, anti-complement, and anti-inflammatory effects. Herein, astragalin-functionalized Cu_<em>2-x</em>Se nanoparticles (CSPA NPs) can effectively inhibit the complement pathway, mitigating microglia-mediated synaptic phagocytosis and promoting synaptic restoration to repair sevoflurane-induced neurotoxicity. They efficiently target and reduce microglial activation and phagocytosis. By downregulating sortilin, CSPA NPs increase progranulin expression, promoting TFEB cytoplasmic translocation to decrease lysosomal activity and microglial phagocytosis. Furthermore, CSPA NPs decrease complement C1q and C3 levels, inhibiting microglial synaptic engulfment and ameliorating cognition dysfunction in sevoflurane-treated mice. This study illustrates that CSPA NPs inhibit microglial synaptic elimination <em>via</em> the complement pathway, alleviating sevoflurane-induced neurotoxicity and providing insights into treating complement pathway-related diseases.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101714"},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk-derived exosomes as functional nanocarriers in wound healing: Mechanisms, applications, and future directions","authors":"Jing Ruan ,&nbsp;Yuping Xia ,&nbsp;Yilei Ma ,&nbsp;Xiyao Xu ,&nbsp;Shihao Luo ,&nbsp;Jia Yi ,&nbsp;Baihui Wu ,&nbsp;Rongbing Chen ,&nbsp;Hanbing Wang ,&nbsp;Honggang Yu ,&nbsp;Qinsi Yang ,&nbsp;Wei Wu ,&nbsp;Da Sun ,&nbsp;Junbo Zhong","doi":"10.1016/j.mtbio.2025.101715","DOIUrl":"10.1016/j.mtbio.2025.101715","url":null,"abstract":"<div><div>Wound healing presents a significant challenge in healthcare, imposing substantial physiological and economic burdens. While traditional treatments and stem cell therapies have shown benefits, milk-derived exosomes (MDEs) offer distinct advantages as a cell-free therapeutic approach. MDEs, isolated from mammalian milk, are characterized by their biocompatibility, ease of acquisition, and high yield, making them a promising tool for enhancing wound repair. This review provides a comprehensive analysis of the composition, sources, and extraction methods of MDEs, with a focus on their therapeutic role in both acute and diabetic chronic wounds. MDEs facilitate wound healing through the delivery of bioactive molecules, modulating key processes such as inflammation, angiogenesis, and collagen synthesis. Their ability to regulate complex wound-healing pathways underscores their potential for widespread clinical application. This review highlights the importance of MDEs in advancing wound management and proposes strategies to optimize their use in regenerative medicine.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101715"},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumbrokinase-containing gelatin nanofibers with multiple bioactivities for effective skin wound healing","authors":"Wen-Ling Wang ,&nbsp;Yi-Hui Lai ,&nbsp;Chiung-Hua Huang ,&nbsp;Jui-Yang Lai ,&nbsp;Chun-Hsu Yao","doi":"10.1016/j.mtbio.2025.101713","DOIUrl":"10.1016/j.mtbio.2025.101713","url":null,"abstract":"<div><div>Wound healing is a highly complex and intricate biological process involving cellular and molecular events. Given that lumbrokinase is a fibrinolytic enzyme derived from earthworms and exhibits notable anti-inflammatory, anti-fibrotic, and pro-angiogenic functions, this study aims to investigate the development of bioactive gelatin nanofibers containing lumbrokinase (GLK) fabricated through electrospinning as a novel nanomedicine strategy for enhancing wound healing. Our results showed that reducing electrospinning time can increase cross-linking degree and decrease degradation rate to maintain an effective concentration of released LK for supporting long-term biological processes. Cells cultured with biocompatible GLK displayed good adhesion and extensive spreading, increased VEGF production, and lowered IL-6 and TNF-α secretion. The GLK with superior and multiple bioactivities was further tested for tissue regeneration potential in a rat model of skin defect. The treatment of animals with GLK shortens wound healing time, reduces damage caused by inflammation, and increases collagen production, angiogenesis, and fibroblast proliferation/epithelialization, demonstrating that the healing effect on the local wounds is comparable to that of Comfeel group. Overall, the findings from preclinical studies suggest high promise of the LK-loaded biopolymer nanofibers as bioactive dressing materials for promoting a regenerative environment and accelerating wound healing, indicating its future translational potential.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101713"},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bundling gold nanorods with RCA-produced DNA tape into an intelligently reconfigurable nanocluster bomb for multimodal precision cancer therapy","authors":"Qian Gao ,&nbsp;Weijun Wang ,&nbsp;Shujuan Sun ,&nbsp;Ya Yang ,&nbsp;Kaili Mao ,&nbsp;Yuxi Yang ,&nbsp;Zai-Sheng Wu","doi":"10.1016/j.mtbio.2025.101718","DOIUrl":"10.1016/j.mtbio.2025.101718","url":null,"abstract":"<div><div>Via proposing an innovative assembly technique, we bundle cell-targeting aptamer-modified gold nanorods (AuNRs) with RCA product (RCA-p) tape into a reconfigurable nanocluster (ARGN) bomb for multimodal precision cancer therapy. Because each ARGN has 10 individual AuNRs, the short time of laser irradiation can make the temperature increase to 75 °C much higher than the lethal temperature of tumor cells, enabling the efficient photothermal therapy (PTT). Moreover, both siRNA-Plk1 (2820 per ARGN) and chemotherapeutic agents (15860 per ARGN) can be loaded into two specifically-designed containers in the internal cavity. Because the glomeroplasmatic structure enhances the resistance to enzymatic degradation, ARGN bomb can protect siRNAs from the digestion and avoid Dox leakage during <em>in vivo</em> circulation. Moreover, the spontaneous structural reorganization allows aptamers in the interior cavity move outward to the exterior surface, which magically offers the compensation of degraded aptamers and impair persistent <em>in vivo</em> cell targeting ability. The external stimuli (laser irradiation) promotes the release of chemotherapeutic agents and initiates the PTT/chemotherapy outcome, while endogenous stimuli (intracellular biomarkers) causes almost 100 % release of siRNA-Plk1 species and induces RNA interference therapy, completely inhibiting tumor growth without detectable off-target toxicity.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101718"},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal-clearable and mitochondria-targeted metal-engineered carbon dot nanozymes for regulating mitochondrial oxidative stress in acute kidney injury","authors":"Jiangpeng Pan ,&nbsp;Juntao Wang ,&nbsp;Wei Wang ,&nbsp;Ziyang Liu ,&nbsp;Shuai Huo ,&nbsp;Lei Yan ,&nbsp;Wei Jiang ,&nbsp;Fengmin Shao ,&nbsp;Yue Gu","doi":"10.1016/j.mtbio.2025.101717","DOIUrl":"10.1016/j.mtbio.2025.101717","url":null,"abstract":"<div><div>Mitochondrial dysfunction-induced oxidative stress is a key pathogenic factor in acute kidney injury (AKI). Despite this, current mitochondrial-targeted antioxidant therapies have shown limited efficacy in clinical settings. In this study, we introduce a novel renal-clearable and mitochondria-targeted antioxidant nanozyme (TPP@RuCDzyme) designed to precisely modulate mitochondrial oxidative stress and mitigate AKI progression. TPP@RuCDzyme was synthesized by integrating ruthenium-doped carbon dots (CDs) with triphenylphosphine (TPP), a mitochondria-targeting moiety. This nanozyme system exhibits cascade enzyme-like activities, mimicking superoxide dismutase (SOD) and catalase (CAT), to efficiently convert cytotoxic superoxide (O₂•^-) and hydrogen peroxide (H₂O₂) into non-toxic water (H₂O) and oxygen (O₂). This dual-enzyme mimicry effectively alleviates mitochondrial oxidative damage, restores mitochondrial function, and inhibits apoptosis. Compared to RuCDzyme alone, TPP@RuCDzyme demonstrated significantly enhanced efficacy in alleviating glycerol-induced AKI by inhibiting oxidative stress. By leveraging the catalytic activity derived from the integration of CDs and a metallic element, this study presents a promising therapeutic strategy for AKI and other renal diseases associated with mitochondrial dysfunction.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101717"},"PeriodicalIF":8.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constructing J-aggregates of cyanine dye for NIR-II in vivo dynamic vascular imaging and long-term targeting of tumors","authors":"Jiaqi Zhou ,&nbsp;Hao Li ,&nbsp;Hui Li ,&nbsp;Jiayi Ding ,&nbsp;Zhong Du ,&nbsp;Jiabao Xiong ,&nbsp;Hongyang Yao ,&nbsp;Xueliang Zhang ,&nbsp;Nuernisha Alifu ,&nbsp;Biao Dong","doi":"10.1016/j.mtbio.2025.101693","DOIUrl":"10.1016/j.mtbio.2025.101693","url":null,"abstract":"<div><div>Cyanine molecules with the second near-infrared (NIR-II) emission hold great potential for bioimaging owing to their great biocompatibility, but the scissor-like structure of these molecules poses a major bottleneck in obtaining efficient NIR-II fluorescence probes. Constructing J-aggregates represents a promising strategy for obtaining biomedical NIR-II emissive materials. However, achieving J-aggregates in cyanine dyes with large torsion angles between the heterocyclic rings poses a challenge. In this study, we introduced the guanidine of tumor molecular targeted peptide 1 (TMTP1) to increase steric hindrance of IR-783 and reduce the angle of IR-783 scissors. The near-coplanar structure of IR-783@peptide TMTP1 composite facilitates the formation of a novel J-aggregates (IR-783-LP-TMTP1) with super-stable effect for NIR-II in vivo dynamic vascular imaging and remarkable tumor targeting capability. The stable emission wavelength and high spatial resolution of J-aggregates was demonstrated for brain and ear vasculature bioimaging under 808 nm laser excitation. Additionally, J-aggregates exhibits robust tumor-targeting capability towards cervical tumors, indicating their potential in cervical cancer diagnosis. This work develops a molecular design strategy to construct bright NIR-II J-aggregates with super-stable and robust tumor-targeting properties and paving the way for improving bioimaging performance of similar molecules.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101693"},"PeriodicalIF":8.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies","authors":"Théo Ziegelmeyer ,&nbsp;Karolinne Martins de Sousa ,&nbsp;Tzu-Ying Liao ,&nbsp;Rodolphe Lartizien ,&nbsp;Alexandra Delay ,&nbsp;Julien Vollaire ,&nbsp;Véronique Josserand ,&nbsp;Denver Linklater ,&nbsp;Phuc H. Le ,&nbsp;Jean-Luc Coll ,&nbsp;Georges Bettega ,&nbsp;Elena P. Ivanova ,&nbsp;Véronique Martel-Frachet","doi":"10.1016/j.mtbio.2025.101710","DOIUrl":"10.1016/j.mtbio.2025.101710","url":null,"abstract":"<div><div>Titanium (Ti) is widely used as an implantable material for bone repair in orthopedics and dentistry. However, Ti implants are vulnerable to bacterial infections, which can compromise patient recovery and lead to implant failure. While a controlled inflammatory response promotes bone regeneration, chronic inflammation caused by infections can lead to implant failure. Bone repair is a complex process in which inflammation, angiogenesis and osteogenesis are tightly interconnected, requiring cooperation between mesenchymal stem cells (MSC), macrophages and endothelial cells. Here, we fabricated bio-inspired Ti implants with either microstructured (Micro Ti) or nanostructured (Nano Ti) surface textures that exhibit robust mechano-bactericidal properties. <em>In vitro</em>, both textured surfaces improved blood coagulation and osteogenic marker expression compared to smooth Ti surfaces. Additionally, Nano Ti promoted macrophage polarization towards the M2 phenotype and enhanced the paracrine effects of MSCs on angiogenesis, key processes in tissue regeneration. <em>In vivo</em> kinetic analysis of bone reconstruction in a rat calvarial model showed that Nano Ti improved osseointegration, as evidenced by increased bone volume, mineral density, and bone-implant contact. Notably, the Micro Ti surface showed no significant differences from the control implants. These findings highlight the potential of mechano-bactericidal surface nanopatterns to simultaneously prevent infections and enhance osseointegration by modulating protein adsorption, inflammation, angiogenesis and osteogenesis. This study provides new insights into the development of bifunctional Ti implants, offering new perspectives for the next generation of implantable bone-related biomaterials.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101710"},"PeriodicalIF":8.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}