Materials Today Bio最新文献

{"title":"Double-targeted liposomes coated with matrix metallopeptidase-2-responsive polypeptide nanogel for chemotherapy and enhanced immunotherapy against cervical cancer","authors":"Zhimin Bai,&nbsp;Yibo Yang,&nbsp;Zutong Cui,&nbsp;Wenming Liang,&nbsp;Xin Zhang,&nbsp;Zihan Zhang,&nbsp;Jianming Sun,&nbsp;Zhiwei Liu,&nbsp;Kun Li,&nbsp;Ming Shi,&nbsp;Jian Li","doi":"10.1016/j.mtbio.2024.101412","DOIUrl":"10.1016/j.mtbio.2024.101412","url":null,"abstract":"<div><div>Immunotherapy is a cornerstone in cancer treatment, celebrated for its precision, ability to eliminate residual cancer cells, and potential to avert tumor recurrence. Nonetheless, its effectiveness is frequently undermined by the immunosuppressive milieu created by tumors. This study presents a novel nanogel-based drug delivery system, DOX-4PI@CpG@Lipo@Gel (DPCLG), engineered to respond to Matrix Metallopeptidase-2 (MMP-2)—a protease abundant in the tumor microenvironment (TME). This system enables the controlled release of two distinct types of liposomes within the TME. The first, DOX-4PI@Liposome (DPL), carries doxorubicin (DOX) and 4-phenylimidazole (4PI), targeting cancer cells to provide chemotherapeutic effects while diminishing the immunosuppressive environment. The second, a mannosyl-modified cationic liposome (CL), is loaded with Cytosine phosphate guanine (CpG) oligodeoxynucleotides to specifically target M2 phenotype macrophages, reversing their tumor-associated phenotype (TAM) and activating immune cytokines to promote tumor destruction. Our findings indicate that DPCLG significantly curtails tumor growth, both in vitro and in vivo, mitigates the immunosuppressive TME, and triggers a potent systemic immune response. This study underscores the potential of DPCLG as an advanced, dual-targeting drug delivery system for comprehensive cancer therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101412"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immunoglobulin of yolk and cerium oxide-based fibrous poly(L-lactide-co-glycolide)/gelatin dressings enable skin regeneration in an infectious wound model","authors":"Xinyuan Zhao ,&nbsp;Changwen Weng ,&nbsp;Hao Feng ,&nbsp;Muhammad Shafiq ,&nbsp;Xinyi Wang ,&nbsp;Lei Liu ,&nbsp;Lu Han ,&nbsp;Mohamed EL-Newehy ,&nbsp;Meera Moydeen Abdulhameed ,&nbsp;Zhengchao Yuan ,&nbsp;Xiumei Mo ,&nbsp;Yanbiao Wang","doi":"10.1016/j.mtbio.2024.101408","DOIUrl":"10.1016/j.mtbio.2024.101408","url":null,"abstract":"<div><div>The bacterial infection and oxidative wound microenvironment delay skin repair and necessitate intelligent wound dressings to enable scarless wound healing. The immunoglobulin of yolk (IgY) exhibits immunotherapeutic potential for the potential treatment of antimicrobial-resistant pathogens, while cerium oxide nanoparticles (CeO₂ NPs) could scavenge superoxide dismutase (SOD) and inflammation. The overarching objective of this study was to incorporate IgY and CeO₂ NPs into poly(L-lactide-co-glycolide)/gelatin (PLGA/Gel)-based dressings (P/G@IYCe) for infected skin repair. The P/G@IYCe manifested good biocompatibility as well as showed significant antibacterial effect against Staphylococcus aureus (<em>S. aureus</em>) and Escherichia coli (<em>E.coil</em>) <em>in vitro</em>. Subcutaneous implantation of membranes in rats exhibited cytocompatibility. Transplantation of membranes in <em>S. aureus</em>-infected full-thickness excisional defects manifested significant beneficial effect of P/G@IYCe dressings than that of the other groups in terms of the scar tissue formation, inflammation resolution, and scavenging of reactive oxygen species (ROS) at 2 weeks post-transplantation. Taken together, the dual delivery of IgY and CeO₂ may enable intelligent wound dressings.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101408"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GSH/pH-responsive copper-based cascade nanocomplexes inducing immunogenic cell death through cuproptosis/ferroptosis/necroptosis in oral squamous cell carcinoma","authors":"Yi Sun ,&nbsp;Enze Li ,&nbsp;Wenzhao Zhong ,&nbsp;Zhaoming Deng ,&nbsp;Ziyao Zhou ,&nbsp;Ka Hong Wong ,&nbsp;Xiangwei Li","doi":"10.1016/j.mtbio.2024.101434","DOIUrl":"10.1016/j.mtbio.2024.101434","url":null,"abstract":"<div><div>Oral squamous cell carcinoma (OSCC) remains a formidable challenge due to high recurrence rates and limited efficacy of conventional treatments. Immunotherapy holds potential, but its effectiveness is often restricted by low patient responsiveness. This study presents a novel therapeutic strategy using GSH/pH-responsive copper-based cascade nanocomplexes to induce immunogenic cell death (ICD) in OSCC. The fabricated nanocomplex, PC@B-H, leverages the acidic and reducing tumor microenvironment to release copper ions and plumbagin, triggering a cascade of cell death mechanisms including cuproptosis, ferroptosis, and necroptosis. This multifunctional system not only enhances oxidative stress but also depletes glutathione, promotes lipid peroxidation, and disrupts mitochondrial function, leading to robust tumor inhibition. Additionally, the induction of ICD facilitates dendritic cell maturation and cytotoxic T lymphocyte infiltration, providing durable anti-tumor immunity. The study demonstrates that PC@B-H achieves a 92.3 % tumor growth inhibition rate with minimal systemic toxicity, offering a promising avenue for enhancing the efficacy of OSCC treatment through combined cell death pathways and immune activation.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101434"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell-derived exosome delivery systems for treating atherosclerosis: The new frontier of stem cell therapy","authors":"Hassan Tariq ,&nbsp;Syeda Zunaira Bukhari ,&nbsp;Ruibing An ,&nbsp;Jian Dong ,&nbsp;Ayesha Ihsan ,&nbsp;Muhammad Rizwan Younis","doi":"10.1016/j.mtbio.2024.101440","DOIUrl":"10.1016/j.mtbio.2024.101440","url":null,"abstract":"<div><div>Cardiovascular diseases (CVDs) are a leading cause of mortality worldwide. As a chronic inflammatory disease with a complicated pathophysiology marked by abnormal lipid metabolism and arterial plaque formation, atherosclerosis is a major contributor to CVDs and can induce abrupt cardiac events. The discovery of exosomes' role in intercellular communication has sparked a great deal of interest in them recently. Exosomes are involved in strategic phases of the onset and development of atherosclerosis because they have been identified to control pathophysiologic pathways including inflammation, angiogenesis, or senescence. This review investigates the potential role of stem cell-derived exosomes in atherosclerosis management. We briefly introduced atherosclerosis and stem cell therapy including stem cell-derived exosomes. The biogenesis of exosomes along with their secretion and isolation have been elaborated. The design engineering of exosomes has been summarized to present how drug loading and surface modification with targeting ligands can improve the therapeutic and targeting capacity of exosomes, demonstrating atheroprotective action. Moreover, the mechanism of action (endothelial dysfunction, reduction of dyslipidemia, macrophage polarization, vascular calcification, and angiogenesis) of drug-loaded exosomes to treat atherosclerosis has been discussed in detail. In the end, a comparative and balanced viewpoint has been given regarding the current challenges and potential solutions to advance exosome engineering for cardiovascular therapeutic applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101440"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial-assisted organoid technology for disease modeling and drug screening","authors":"Yunyuan Shao ,&nbsp;Juncheng Wang ,&nbsp;Anqi Jin ,&nbsp;Shicui Jiang ,&nbsp;Lanjie Lei ,&nbsp;Liangle Liu","doi":"10.1016/j.mtbio.2024.101438","DOIUrl":"10.1016/j.mtbio.2024.101438","url":null,"abstract":"<div><div>Developing disease models and screening for effective drugs are key areas of modern medical research. Traditional methodologies frequently fall short in precisely replicating the intricate architecture of bodily tissues and organs. Nevertheless, recent advancements in biomaterial-assisted organoid technology have ushered in a paradigm shift in biomedical research. This innovative approach enables the cultivation of three-dimensional cellular structures <em>in vitro</em> that closely emulate the structural and functional attributes of organs, offering physiologically superior models compared to conventional techniques. The evolution of biomaterials plays a pivotal role in supporting the culture and development of organ tissues, thereby facilitating more accurate disease state modeling and the rigorous evaluation of drug efficacy and safety profiles. In this review, we will explore the roles that various biomaterials play in organoid development, examine the fundamental principles and advantages of utilizing these technologies in constructing disease models, and highlight recent advances and practical applications in drug screening using disease-specific organoid models. Additionally, the challenges and future directions of organoid technology are discussed. Through continued research and innovation, we aim to make remarkable strides in disease treatment and drug development, ultimately enhancing patient quality of life.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101438"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antitumor efficacy of STING agonist MSA-2 by lipid nanoparticles delivering circular IL-23 mRNA and platinum-modified MSA-2 combination","authors":"Tian He ,&nbsp;Yating Li ,&nbsp;Weiqi Li ,&nbsp;Muqing Zhang ,&nbsp;Guishuan Wang ,&nbsp;Peng Zhou ,&nbsp;Guoqi Song ,&nbsp;Wenqing Li","doi":"10.1016/j.mtbio.2025.101446","DOIUrl":"10.1016/j.mtbio.2025.101446","url":null,"abstract":"<div><div>A next-generation STING agonist MSA-2 is a promising tumor immunotherapy strategy. However, the methods for improving the anti-tumor efficacy of MSA-2 are a lot of effort. We have demonstrated antitumor effect of platinum-modified MSA-2 (MSA-2-Pt) was better than MSA-2. Here, we combined lipid nanoparticles delivering circular IL-23 mRNA (LNP@cIL-23) and MSA-2-Pt strategy, which showed good antitumor efficacy. Firstly, we synthesized a new series of ionizable phospholipids and formulated and optimized an LNP36 for delivering circular IL-23 mRNA. Then, the combination of LNP36@cIL-23 mRNA and MSA-2-Pt induced tumor cell death and immune activation in the tumor with a single i.t. injection. Finally, the combination of LNP36@cIL-23 mRNA and MSA-2-Pt significantly decreased the melanoma B16F10 tumor and prolonged the survival, demonstrating significant anti-tumor effects. This finding provides promising new avenues for STING activation strategies in tumor immunotherapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101446"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent development of micro-nano carriers for oral antineoplastic drug delivery","authors":"Hongzheng Li ,&nbsp;Xiang Chen ,&nbsp;Shangrui Rao ,&nbsp;Minyu Zhou ,&nbsp;Jianhua Lu ,&nbsp;Danna Liang ,&nbsp;Bingzi Zhu ,&nbsp;Letian Meng ,&nbsp;Ji Lin ,&nbsp;Xiaoya Ding ,&nbsp;Qingfei Zhang ,&nbsp;Danhong Hu","doi":"10.1016/j.mtbio.2025.101445","DOIUrl":"10.1016/j.mtbio.2025.101445","url":null,"abstract":"<div><div>Chemotherapy is widely recognized as a highly efficacious modality for cancer treatment, involving the administration of chemotherapeutic agents to target and eradicate tumor cells. Currently, oral administration stands as the prevailing and widely utilized method of delivering chemotherapy drugs. However, the majority of anti-tumor medications exhibit limited solubility and permeability, and poor stability in harsh gastrointestinal environments, thereby impeding their therapeutic efficacy for chemotherapy. Therefore, more and more micro-nano drug delivery carriers have been developed and used to effectively deliver anti-cancer drugs, which can overcome physiological barriers, facilitate oral administration, and ultimately improve drug efficacy. In this paper, we first discuss the effects of various biological barriers on micro-nano drug carriers and oral administration approach. Then, the development of micro-nano drug carriers based on various biomedical components, such as micelles, dendrimers, hydrogels, liposomes, inorganic nanoparticles, etc. were introduced. Finally, the current dilemma and the potential of oral drug delivery for clinical treatment were discussed. The primary objective of this review is to introduce various oral delivery methods and serve as a point of reference for the advancement of novel oral delivery carriers, with the ultimate goal of informing the development of future clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101445"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A screening setup to streamline in vitro engineered living material cultures with the host","authors":"Krupansh Desai ,&nbsp;Shrikrishnan Sankaran ,&nbsp;Aránzazu del Campo ,&nbsp;Sara Trujillo","doi":"10.1016/j.mtbio.2024.101437","DOIUrl":"10.1016/j.mtbio.2024.101437","url":null,"abstract":"<div><div>Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. Determining the conditions that sustain ELM performance while ensuring compatibility with ELM hosts is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through <em>in vitro</em> investigations. Currently, there are no standards that ensure ELM compatibility with host tissues. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential <em>in vitro</em>. We showed proliferation of three bacterial species encapsulated in hydrogels over time and screened six different cell culture media. We fabricated ELMs in bilayer and monolayer formats and tracked bacterial leakage as a measure of ELM biocontainment. After screening, an appropriate medium was selected that sustained growth of an ELM, and it was used to study cytocompatibility <em>in vitro</em>. ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. Our work illustrates a simple setup to streamline the screening of compatible environmental conditions of ELMs with the host.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101437"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy","authors":"Hongyan Li ,&nbsp;Rong Gan ,&nbsp;Jiadi Liu ,&nbsp;Duling Xu ,&nbsp;Qiyue Zhang ,&nbsp;Haidong Tian ,&nbsp;Huijun Guo ,&nbsp;Haijun Wang ,&nbsp;Zhimin Wang ,&nbsp;Xianwu Zeng","doi":"10.1016/j.mtbio.2025.101455","DOIUrl":"10.1016/j.mtbio.2025.101455","url":null,"abstract":"<div><div>Liposomal nanocarriers are able to carry peptides for efficient and selective delivery of radioactive tracer and drugs into the tumors. Angiopoietin 2 (ANGPT2) is an excellent biomarker for precise diagnosis and therapy of glioma. The present study aimed to design ANGPT2-specific peptides to modify the surface of nanoliposomes containing doxorubicin (Dox) for integrative imaging and targeting therapy of glioma. The targeted ANGPT2 peptides were designed using the molecular operating environment. Peptide-conjugated PEGlated liposomes containing Dox (peptide-Lipo@Dox) were prepared for radionuclide and drug delivery. Glioma cell functions were determined based on cell cycle and viability, apoptosis, cell invasion and migration, and colony-formation assays. The anti-tumor effect of peptide-Lipo@Dox was validated in intracranial U87-MG cell glioma-bearing mice <em>in vivo</em>. The peptides GSFIHSVPRH (GSF) and HSVPRHEV (HSV) showed specific affinity for ANGPT2 and a better cellular uptake in U87-MG cells. Micro-positron emission tomography (PET)/computed tomography (CT) imaging was used to visualize the orthotopic transplantation of glioma in the brain 1 h after injection of radionuclide ⁶⁸Ga-labeled peptide-Lipo@Dox. Lipo@Dox with peptide modification demonstrated stable Dox loading, small sizes (&lt;40 nm), and enrichment in the tumor region of the mouse brain. Peptide-Lipo@Dox treatment inhibited the Tie-2/Akt/Foxo-1 pathway, thereby inhibiting cell invasion and migration, cell viability, and colony-forming ability of U87-MG cells. Lipo@Dox peptide modification showed a better suppression of glioma development than Lipo@Dox. Thus, the ANGPT2-specific peptides were successfully designed, and the PEGylated liposome modified with ANGPT2-specific peptide served as part of a potent delivery method for integrative glioma-targeted imaging and therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101455"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy","authors":"Mingzhi Liu ,&nbsp;Zhiyong Cui ,&nbsp;Derong Xu ,&nbsp;Chenguang Liu ,&nbsp;Chuanli Zhou","doi":"10.1016/j.mtbio.2025.101537","DOIUrl":"10.1016/j.mtbio.2025.101537","url":null,"abstract":"<div><div>Discectomy is a widely utilized approach for alleviating disc herniation; however, effective repair of postoperative annulus fibrosus (AF) defects remains a significant challenge. This study introduces a hydrogel patch with enhanced mechanical properties for AF repair fabricated using chitin (Ch), collagen (Col), and chitin nanocrystals (ChNCs) through a freeze-thaw cycling technique. The Ch and Col components constitute the matrix of the hydrogel patch, while uniformly dispersed ChNCs act as a nanofiller, markedly improving the mechanical performance (compression strain: 95 %; compression modulus: 0.27 MPa) of the resulting Ch/Col@ChNCs hydrogel patch. The patch demonstrates advantageous properties, including high porosity, superior water absorption, thermal stability, and biodegradability in simulated body fluid. In vitro assessments reveal excellent biocompatibility with AF cells and enhanced collagen deposition. Furthermore, in vivo studies confirm that the patch effectively repairs postoperative disc defects, exhibiting strong integration with surrounding tissues and facilitating the orderly regeneration of fibrous tissue. This innovative hydrogel patch, combining exceptional properties with a straightforward fabrication process, presents a viable strategy for advancing clinical biomaterials for postoperative AF repair.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"31 ","pages":"Article 101537"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}