Materials Today Bio最新文献

{"title":"Oral delivery of teriparatide utilizing biocompatible transferrin-engineered MOF nanoparticles for osteoporosis therapy","authors":"Renxiong Wei ,&nbsp;Sang Hu ,&nbsp;Jiazhi Wang ,&nbsp;Qingjian Lei ,&nbsp;Zhiyu Jiang ,&nbsp;Bo Wang ,&nbsp;Haixia Yang ,&nbsp;Feifei Yan ,&nbsp;Lin Cai ,&nbsp;Jian Tian","doi":"10.1016/j.mtbio.2025.102318","DOIUrl":"10.1016/j.mtbio.2025.102318","url":null,"abstract":"<div><div>Osteoporosis, a systemic skeletal disorder characterized by reduced bone density and increased fracture risk, poses a significant global health challenge. While teriparatide (TRP), a first-line anabolic peptide drug, demonstrates substantial therapeutic benefits in osteoporosis management, its clinical use is restricted by the necessity for daily subcutaneous administration, leading to suboptimal patient compliance. To overcome this limitation, we developed an orally deliverable TRP formulation using biocompatible metal-organic framework nanoparticles (MOF-808 NPs) co-loaded with TRP and functionalized with transferrin targeting ligands (M@P@T NPs). The rationally designed nanoporous architecture coupled with transferrin surface modification synergistically protects TRP from acidic and enzymatic degradation in harsh gastrointestinal environments, while realizing controlled release of TRP in the phosphate-rich bloodstream. Leveraging the overexpression of transferrin receptors (TfR) on intestinal epithelial cells, the nanosystem facilitates receptor-mediated transcellular transport, enabling efficient systemic delivery of TRP with high oral bioavailability. After the one-month oral administration of low-dose M@P@T (200 μg kg⁻¹·day⁻¹) to osteoporosis model mice, therapeutic outcomes comparable to those achieved with subcutaneous TRP injections were observed, including increased bone mineral density, improved trabecular structure, and significant alleviation of osteoporosis symptoms. These findings suggest that this MOF-based oral TRP strategy has great potential for simplifying and improving the treatment of osteoporosis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102318"},"PeriodicalIF":10.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-responsive 2D niobium carbide nanosheets for targeted circPUM1 siRNA delivery in ovarian cancer therapy","authors":"Xue Guan ,&nbsp;Li-li Wang ,&nbsp;Zhuo Yang ,&nbsp;Yang Wang ,&nbsp;Yang Zhao ,&nbsp;Danbo Wang","doi":"10.1016/j.mtbio.2025.102314","DOIUrl":"10.1016/j.mtbio.2025.102314","url":null,"abstract":"<div><div>Ovarian cancer progression is closely associated with tumor microenvironment (TME) dysregulation, particularly pathological angiogenesis driven by exosome-mediated crosstalk. Here, we elucidate that ovarian cancer-derived circPUM1 promotes angiogenesis by transferring to vascular endothelial cells via exosomes. Mechanistically, circPUM1 upregulates the expression of RAB27B and VEGFA by sponging miR-607, thus boosting release of exosome facilitated by RAB27B and directly activating VEGF signaling in endothelial cells to foster angiogenesis. To disrupt this circPUM1-driven TME modulation, we engineered an innovative pH-responsive 2D niobium carbide nanosheets loaded with circPUM1 siRNA. Through excessive PEI grafting, we functionalized the nanosheets with cationic property, achieving efficient negative-charged siRNA loading. Further surface PEGylation shielded the nanosheets’ positive charge, reducing off-target effect and systemic toxicity, while acidic TME triggered PEG exfoliation for tumor-specific circPUM1 siRNA delivery. Functional cellular assays and an intraperitoneal tumor-bearing mouse model validated that the nanosheet-delivered circPUM1 siRNA effectively inhibited angiogenesis and peritoneal dissemination by knocking down circPUM1 expression and subsequently downregulating its downstream targets. This study uncovers a novel exosome-mediated angiogenesis mechanism and develops innovative MXene nanosheets for pH-responsive siRNA delivery providing a promising strategy for ovarian cancer precision therapy with significant clinical translational value and application potential.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102314"},"PeriodicalIF":10.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple and high-containment lung-on-chip model for studying respiratory viral infections using human primary lung cells","authors":"David Barata ,&nbsp;Sem Koornneef ,&nbsp;Francesca Giacomini ,&nbsp;Zeinab Niloofar Tahmasebi Birgani ,&nbsp;Jiangrong Zhou ,&nbsp;Pengfei Li ,&nbsp;Robbert J. Rottier ,&nbsp;Roman K. Truckenmüller","doi":"10.1016/j.mtbio.2025.102316","DOIUrl":"10.1016/j.mtbio.2025.102316","url":null,"abstract":"<div><div>Airborne respiratory viruses, such as coronaviruses and influenza, pose major threats to public health and the economy, as highlighted by the COVID-19 pandemic. Preclinical research is hindered by models that poorly mimic human tissue structure and function, often relying on immortalized cell lines and low-throughput animal studies. This limits accurate prediction of disease mechanisms, drug effects, and target suitability. Here, we report a custom-engineered, passive-flow, high-containment chip for culturing human primary bronchial epithelial cells (hPBECs) at air-liquid interface (ALI) on a large-area membrane. The dual-chamber microfluidic chip, separated by a horizontal support membrane, is enclosed in a 35 mm sealed Petri dish, enabling safe use in standard incubators without leakage or biosafety concerns. The platform supports high-resolution in-situ imaging, apical viral infection, and retrieval of cells and secretions (e.g., mucus, viral lysate) for molecular analysis. We demonstrate robust infection and replication of human coronavirus NL63 (HCoV-NL63) in differentiated hPBECs cultured up to 4 weeks at ALI. Epithelial differentiation was confirmed by immunofluorescence (e.g., ciliated cells), and infection kinetics were monitored by RT-qPCR over 7 days. The interferon-based immune response showed increased activity, with upregulation of viral response pathways (e.g., replication, inflammation, immunoregulation), and consistent activation across donors (e.g., ISG15, IFIT1). Collectively, we present a reproducible, small-scale chip model that enables high-containment <em>in vitro</em> studies of respiratory viruses and their effects on human airway epithelia.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102316"},"PeriodicalIF":10.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel folate receptor-targeted curcumin nano-delivery system: A dual therapeutic strategy for precise inhibition of MRCKβ to suppress ovarian cancer proliferation and immune escape","authors":"Mengna Shi ,&nbsp;Lulu Chen ,&nbsp;Yang Yu ,&nbsp;Hong Wang ,&nbsp;Min-Jie Zhang","doi":"10.1016/j.mtbio.2025.102307","DOIUrl":"10.1016/j.mtbio.2025.102307","url":null,"abstract":"<div><div>Curcumin is a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, exhibiting diverse biological activities, including anti-inflammatory, antioxidant, antitumor, antibacterial, antiviral, and neuroprotective effects. However, its clinical application is severely limited by poor oral absorption and low bioavailability. To address these challenges, this study synthesized a novel folate-targeted curcumin-loaded material—CU-FRα/PEG-2-CHO. This delivery system significantly enhances tumor cell uptake efficiency through folate receptor (FRα)-mediated active targeting (FRα denotes the folate receptor alpha subtype). Additionally, polyethylene glycol (PEG) modification and aldehyde (-CHO) functionalization further improve its water solubility and stability. Experimental results demonstrate that this material not only substantially increases curcumin's absorption rate and bioavailability but also enhances its antitumor activity via targeted delivery mechanisms. This study developed a new type (significantly improving various performances) folate receptor-targeted curcumin nano-delivery system (CU-FRα/PEG-2-CHO) and systematically compared the antitumor effects of two curcumin nanomaterials (CU-FRα/PEG-1-CHO and CU-FRα/PEG-2-CHO), demonstrating that CU-FRα/PEG-2-CHO significantly inhibited the growth of A2780 and HO8910PM ovarian cancer cell lines. After targeting tumor cells, CU-FRα/PEG-2-CHO released curcumin, which was mechanistically shown to specifically target Cdc42-binding kinase β (MRCKβ, IC₅₀ = 2.12 μM), with its binding characteristics confirmed by surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and cellular thermal shift assay (CESTA). Molecular docking analysis revealed that MRCKβ precisely binds to the ATP-binding pocket of curcumin. Further transcriptomic analysis demonstrated that MRCKβ silencing significantly affected the TGF-β-SMAD4-LAG-3 signaling pathway, and CU-FRα/PEG-2-CHO exerted its therapeutic effects by regulating the MRCKβ-APC-SMAD4-CTLA-4 signaling axis to suppress tumor cell proliferation and immune evasion. Animal experiments confirmed that CU-FRα/PEG-2-CHO administration at a dose of 1 mg/kg every two days significantly inhibited tumor growth with a dose-dependent effect. Notably, although cisplatin (5 mg/kg every two days) and αPD-L1 (5 mg/kg every two days) also showed certain therapeutic effects, their tumor suppression efficacy was significantly lower than that of CU-FRα/PEG-2-CHO. These findings not only elucidate the mechanism of a novel folate receptor-targeted curcumin nanoliposome but also provide important evidence for developing MRCKβ-targeted therapeutic strategies against ovarian cancer.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102307"},"PeriodicalIF":10.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-Reinforced Dual-Crosslinked Pueraria Hydrogel Coating for Synergistic Atherosclerosis Intervention","authors":"Jingyue Wang ,&nbsp;Ge Yin ,&nbsp;Leila Mamizadeh Janghour ,&nbsp;Sheng Dai ,&nbsp;Behnam.Akhavan ,&nbsp;Maolin Sun ,&nbsp;Ansha Zhao","doi":"10.1016/j.mtbio.2025.102313","DOIUrl":"10.1016/j.mtbio.2025.102313","url":null,"abstract":"<div><div>To address the clinical challenges of stent thrombosis and restenosis in atherosclerosis intervention, this study developed a dual-crosslinked hydrogel coating (PSMASH—integrating methacrylated Pueraria polysaccharide (PSMA) and thiolated derivative (PSSH) based on Pueraria lobata polysaccharide). We used an ion-assisted plasma polymerization (IAPP) technique to construct micro-nano structures and thiol-ene click reaction sites on the surface of 316L stainless steel stents. This approach enabled robust covalent integration between the coating and the substrate. Simultaneously, the dual-network architecture of the hydrogel coating (synergistic covalent and dynamic crosslinking) conferred enhanced mechanical stability and controlled degradation properties. PSMASH promoted endothelial cell recovery and modulated the pathological phenotype of smooth muscle cells under oxidative stress. This effect was mediated by the controlled release of Pueraria-derived flavonoids. This \"herb-to-device\" (HTD) strategy integrates traditional Chinese medicinal components with advanced interface engineering. It establishes a novel therapeutic platform for atherosclerosis intervention that offers superior mechanical compatibility and multi-target synergistic efficacy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102313"},"PeriodicalIF":10.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed nHA/PA66 porous scaffold: Regulating immune balance and vascularization synergistically promotes bone regeneration","authors":"Caiping Yan ,&nbsp;Fukang Zhu ,&nbsp;Hao Liang ,&nbsp;Changxing Liu ,&nbsp;Bin He ,&nbsp;Taiyou Wang ,&nbsp;Heling Tan ,&nbsp;Hong Li ,&nbsp;Dianming Jiang ,&nbsp;Bo Qiao","doi":"10.1016/j.mtbio.2025.102315","DOIUrl":"10.1016/j.mtbio.2025.102315","url":null,"abstract":"<div><div>3D-printed porous scaffolds have emerged as a potential solution for treating critical-sized bone defects, yet localized inflammatory imbalance leads to suboptimal therapeutic outcomes. This study utilized nHA/PA66 composite material as the foundation, employing APF additive manufacturing technology to fabricate a three-dimensional porous scaffold (HP) suitable for local bone filling, and functionalized the porous scaffold through modification with polydopamine and magnesium ions (Mg²⁺) (HPDM). First, the physicochemical properties and biosafety of the HPDM scaffold were verified, followed by a comparative analysis of its effects on macrophage phenotype transformation, vascular endothelial cell differentiation, and pre-osteoblast differentiation differences. The HPDM scaffold achieves coordinated balance in the transformation between M1 and M2 macrophages through sustained release of polydopamine and Mg²⁺. Mg²⁺ plays a crucial role in inflammatory regulation by downregulating the NF-κB signaling pathway. Furthermore, several experiments demonstrated that the HPDM scaffold regulates orderly inflammatory responses to promote intercellular interactions, stimulating angiogenesis and osteogenic regeneration. In New Zealand rabbits' femoral condyle bone defect model, the HPDM porous scaffold achieved significant vascularized bone regeneration. This study confirms that the functionalized HPDM porous scaffold prepared using nHA/PA66 as the base material has significant potential in regulating immune responses and enhancing vascularized bone regeneration.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102315"},"PeriodicalIF":10.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myosin-actin pattern links matrix stiffness to GFAT2-hyaluronan metabolism","authors":"Yuwei Zhou ,&nbsp;Yifei Zheng ,&nbsp;Biao Sheng ,&nbsp;Jian Wang ,&nbsp;Kefeng Ding ,&nbsp;Baohua Ji ,&nbsp;Yu Wu","doi":"10.1016/j.mtbio.2025.102305","DOIUrl":"10.1016/j.mtbio.2025.102305","url":null,"abstract":"<div><div>While dysregulated extracellular matrix deposition and stiffening are known to drive tumor progression, breast cancer cells can persist, relapse, and metastasize to soft microenvironments. The distinct strategies that tumor cells adapted to soft matrices remain to be further explored. Here, we report that breast tumor cells exploit soft matrices to activate GFAT2-mediated hyaluronan metabolism that can modulate macrophages. This process is driven by the upregulation of GFAT2 expression through enhanced nuclear translocation of NF-κB and XBP1s, coupled with elevated GFAT activity and subsequent hyaluronan production via suppressed AMPKα phosphorylation. Mechanistically, the expression and activity of GFAT2 are jointly modulated by the total cellular levels of myosin and F-actin. More specifically, the ROCK-Rac1 balance, which can regulate both the cortical-to-cytoplasmic ratio of active myosin and the circumferential arrangement of cortical F-actin, mediates the NF-κB-XBP1s-GFAT2 signaling axis. Furthermore, our <em>in silico</em> modeling validates that the spatial pattern of myosin directly regulates the orientation of cortical F-actin arrangement. These findings establish a novel mechano-metabolic link between matrix compliance and detrimental mediators—NF-κB/XBP1s, GFAT, and hyaluronan—uncovering a potential microenvironmental reprogramming strategy adopted by tumor cells in soft niches.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102305"},"PeriodicalIF":10.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Realization of differential release of minocycline hydrochloride from electrosprayed polymeric or biomacromolecular microparticles for the repair of traumatic brain injury","authors":"Qingxia Guo ,&nbsp;Yue Wang ,&nbsp;Manfei Fu ,&nbsp;Ziyi Zhou ,&nbsp;Xiaopei Zhang ,&nbsp;Siyu Chen ,&nbsp;Cong Xu ,&nbsp;Yuanfei Wang ,&nbsp;Tong Wu","doi":"10.1016/j.mtbio.2025.102309","DOIUrl":"10.1016/j.mtbio.2025.102309","url":null,"abstract":"<div><div>Traumatic brain injury (TBI) occurs when an external force impacts the brain and can result in various serious consequences. Currently, there are no clinical therapies to satisfy the different pathophysiological stages of TBI, realizing a combination of anti-inflammatories in the acute stage and polarization of M2 phenotype microglia. Herein, poly(lactic-co-glycolic acid) (PLGA) and silk fibroin (SF) were selected as shell layers, respectively, to fabricate minocycline hydrochloride (MH)-loaded core-shell microparticles through coaxial electrospray. MH@SF and MH@PLGA microparticles exhibited a differential release profile to promote nerve repair and regeneration after TBI. MH@SF achieved a fast release in 7 days to suppress neuroinflammatory response, while the sustained release of MH@PLGA up to 25 days allowed for modulating polarization of M2 phenotype microglia. The obtained microparticles promoted cell viability and neurite growth of primary neurons and SH-SY5Y cells by establishing neurite transection (NT) and oxygen-glucose deprivation (OGD) models to simulate primary and secondary injury after TBI. <em>In vivo</em> experiments further proved that MH@SF and MH@PLGA microparticles alleviate the neuroinflammation microenvironment and enhance motor, learning, and memory functions of mice after TBI. In summary, this work proposed a promising strategy to regulate the neuroimmune microenvironment through matching different pathophysiological stages of TBI, demonstrating potential for clinical translation to treat TBI.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102309"},"PeriodicalIF":10.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ceria nanoparticles guard against glucocorticoid induced bone homeostasis imbalance through the promotion of osteogenesis and inhibition of osteoclastogenesis","authors":"Anwei Zhang ,&nbsp;Zhiwei Liu ,&nbsp;Ling Zou ,&nbsp;Lu Yang ,&nbsp;Yonghui Wu ,&nbsp;Xinxing Wang ,&nbsp;Daigui Cao ,&nbsp;Junli Liu ,&nbsp;Xiaochao Yang ,&nbsp;Shengli Zhang","doi":"10.1016/j.mtbio.2025.102308","DOIUrl":"10.1016/j.mtbio.2025.102308","url":null,"abstract":"<div><div>Osteoporosis induced by the long-term administration of glucocorticoids is a common clinical problem. Current anti-osteoporosis therapies mainly focus on bone resorption inhibition or bone formation promotion unilaterally. However, the imbalance between bone formation and resorption often leads to suboptimal therapeutic outcomes. This study confirms the pathways through which dexamethasone (DEX), a typical glucocorticoid, triggers GIO, and explores both the therapeutic potential and underlying mechanisms of ceria nanoparticles (CNPs) in mitigating GIO. Our results demonstrate that DEX-induced bone homeostasis imbalance is the primary mechanism underlying GIO development in mice. Specifically, DEX inhibits pre-osteoblast proliferation and differentiation by inducing apoptosis and ferroptosis. Concurrently, DEX promotes osteoclast precursor proliferation and differentiation, significantly accelerating bone resorption. Administration of CNPs significantly mitigates DEX-induced disruption of bone homeostasis by ameliorating intracellular reactive oxygen species (ROS) accumulation via regulation of the Keap1/Nrf2 signaling pathway. Regarding osteogenesis, CNPs are capable of alleviating DEX-induced pre-osteoblast apoptosis and ferroptosis through the regulation of GPX4/ACSL4 signaling pathway. In terms of bone resorption, CNPs inhibit the RANKL-dependent osteoclast formation pathway. These findings validate the potential application of inorganic nanoparticles in GIO prevention.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102308"},"PeriodicalIF":10.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nanoparticles alleviate cobalt toxicity in artificial joint metal prostheses by inhibiting ferroptosis through activation of the PRDX6/GPX4 pathway","authors":"Pengcheng Xu ,&nbsp;Fan Liu ,&nbsp;Su Jiang ,&nbsp;Baisheng Cai ,&nbsp;Cong Ye ,&nbsp;Yiming Sun ,&nbsp;Yaping Wang ,&nbsp;Jining Shen ,&nbsp;Huan Zhou ,&nbsp;Yake Liu","doi":"10.1016/j.mtbio.2025.102306","DOIUrl":"10.1016/j.mtbio.2025.102306","url":null,"abstract":"<div><div>The long-term implantation of metallic joint prostheses results in the release of cobalt nanoparticles (CoNPs), leading to local and even systemic toxic reactions that pose risks to patient health. Previous studies have suggested that CoNPs-induced cytotoxicity may be associated with excessive oxidative stress and ferroptosis. Selenium nanoparticles (SeNPs), known for their anti-ferroptotic properties, have potential as surface coatings for metal implants. This study aims to investigate the role and mechanisms of SeNPs in inhibiting ferroptosis and mitigating cobalt-induced toxicity, thereby offering a mechanistic rationale for improving the material properties of metal prostheses. We first conducted molecular analyses on tissue samples from patients undergoing hip joint revision surgery, which revealed activation of ferroptosis-related signaling pathways. We then synthesized SeNPs capable of effective internalization by bone marrow-derived stromal cells (BMSCs). In vitro, 400 μM CoNPs induced hallmark features of ferroptosis in BMSCs by suppressing the SLC7A11/GPX4 axis and activating the HIF-1α/HO-1 signaling pathway. In contrast, treatment with 40 μM SeNPs upregulated PRDX6 and GPX4, thereby attenuating ferroptosis and preserving cell viability. Finally, intra-articular injection of SeNPs into mouse knee joints significantly alleviated CoNPs-induced local toxic responses, including synovial hyperplasia and cartilage destruction. Overall, this study provides novel insights into the ferroptosis-dependent mechanisms underlying CoNPs-induced toxicity and highlights the therapeutic potential of SeNPs as a detoxifying agent. These findings offer a mechanistic foundation for targeted detoxification strategies and inform the development of improved metal prosthetic materials.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102306"},"PeriodicalIF":10.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}