Materials Today Bio最新文献

{"title":"Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration","authors":"Xiaoming Li ,&nbsp;Shuang Yang ,&nbsp;Shidan Li ,&nbsp;Pengfei Wu ,&nbsp;Wenhui Hu ,&nbsp;Wei Dai ,&nbsp;Jingru Xie ,&nbsp;Jinlong Qiu ,&nbsp;Liang Zhang ,&nbsp;Hui Zhao ,&nbsp;Shiwu Dong","doi":"10.1016/j.mtbio.2025.101967","DOIUrl":"10.1016/j.mtbio.2025.101967","url":null,"abstract":"<div><div>The periosteum is critical for bone reconstruction. Despite serving as a clinical “induced periosteum” treatment for bone defects, the induced membrane technique is associated with significant psychological distress and economic burden due to the need for secondary surgery. Inspired by the ability of induced membranes to function like the periosteum, we propose a tissue-engineered periosteum to replace induced membranes for bone regeneration. This study confirmed that the induced membrane and periosteum share similar architectural and biological properties, including a loose inner layer, a dense outer layer, and a protein expression pattern. An asymmetric nanofibrous membrane was fabricated by electrospinning using gelatin and chitosan, with bone morphogenetic protein (BMP-2) and functionalized hydroxyapatite (Func-HA) incorporated to construct a biomimetic periosteum featuring a reverse biogradient for bone reconstruction and regeneration. The reverse biogradient biomimetic periosteum could significantly enhance osteogenesis and angiogenesis. Interestingly, the biomimetic periosteum also provided a periosteum-mimetic microenvironment by enhancing periosteal stem cells (PSCs) recruitment to the bone defect region and upregulating periostin expression. Our findings suggest that biomimetic membranes with a reverse biogradient could be promising alternatives to induced membranes.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101967"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing cancer therapy: Nanomaterial-based encapsulation strategies for enhanced delivery and efficacy of curcumin","authors":"Yuxing Yan ,&nbsp;Kulsoom ,&nbsp;Yanbo Sun ,&nbsp;Yingjie Li ,&nbsp;Zhenlong Wang ,&nbsp;Li Xue ,&nbsp;Fu Wang","doi":"10.1016/j.mtbio.2025.101963","DOIUrl":"10.1016/j.mtbio.2025.101963","url":null,"abstract":"<div><div>Curcumin, a polyphenolic compound derived from <em>Curcuma longa</em>, has gained significant attention as a potential anticancer agent due to its anti-inflammatory, antioxidant, and antitumor properties. Despite its therapeutic potential, the clinical application of curcumin is limited by its poor aqueous solubility, rapid metabolism, and limited bioavailability. To address these limitations, various nanomaterial-based encapsulation strategies have been developed, including polymeric nanoparticles, liposomes, solid lipid nanoparticles, micelles, dendrimers, and hybrid nanomaterials. These formulations aim to improve curcumin's solubility, stability, cellular uptake, and controlled release, thereby enhancing its targeted delivery to tumor sites. Such approaches not only reduce systemic toxicity but also improve therapeutic efficacy. Recent studies demonstrate that curcumin-loaded nanocarriers exhibit enhanced antitumor effects, selective cytotoxicity toward cancer cells, and minimized side effects. However, challenges such as achieving tissue specificity, evaluating potential toxicity, and the need for thorough clinical validation persist. Future research should prioritize the development of tissue-specific delivery systems, assess safety profiles, and ensure biocompatibility to optimize curcumin's clinical efficacy. This review provides an overview of the latest advancements in curcumin nanocapsules, critically comparing their advantages and limitations in cancer therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101963"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DMAHDM@MPC nanoparticles in orthodontic adhesive inhibit cariogenic bacteria and sugar metabolism to prevent enamel demineralization","authors":"Chengjun Su ,&nbsp;Mengyao Zhu ,&nbsp;Yiman Guo ,&nbsp;Jiachen Sun ,&nbsp;Miao Liu ,&nbsp;Yansong Ma ,&nbsp;Yan Xu ,&nbsp;Yuxing Bai ,&nbsp;Xiaoxia Che ,&nbsp;Ning Zhang","doi":"10.1016/j.mtbio.2025.101969","DOIUrl":"10.1016/j.mtbio.2025.101969","url":null,"abstract":"<div><div>During orthodontic treatment, poor oral hygiene often facilitates the proliferation of cariogenic bacteria, particularly <em>Streptococcus mutans</em>, leading to lactic acid accumulation and subsequent enamel demineralization. To mitigate this issue, Dimethylaminohexadecyl methacrylate (DMAHDM) was incorporated onto the protein-repellent surface of 2-Methacryloyloxyethyl phosphorylcholine (MPC), resulting in the formation of a DMAHDM@MPC composite. This composite was then integrated into resin-modified glass ionomer cement (RMGIC) to develop an antimicrobial orthodontic adhesive, termed RMGIC + MPC + DMAHDM (RMD). This study demonstrated that DMAHDM@MPC nanoparticles self-assembled into a core-shell structure, thereby enhancing the antimicrobial activity. A six-month randomized controlled trial (RCT) involving 29 orthodontic patients, along with metagenomic and metabolomic analyses, revealed that RMD significantly reduced plaque accumulation by selectively inhibiting pathogenic bacteria while preserving beneficial microbiota. Additionally, MPC was shown to competitively bind to sucrose-6-phosphatase (SPP) in pathogenic bacteria, inhibiting sucrose synthesis and carbohydrate metabolism, thus reducing the production of organic acids. In conclusion, RMD effectively prevents enamel demineralization by selectively targeting cariogenic bacteria and their associated sugar metabolism pathways during orthodontic treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101969"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk fibroin hydrogel with recombinant silk fibroin/NT3 protein enhances wound healing by promoting type III collagen synthesis and hair follicle regeneration in skin injury","authors":"Yingying Yan ,&nbsp;Mingxuan Li ,&nbsp;Longyu Guo ,&nbsp;Wenxue Zhang ,&nbsp;Ronghua Wu ,&nbsp;Tuchen Guan ,&nbsp;Jue Ling ,&nbsp;Yumin Yang ,&nbsp;Mei Liu ,&nbsp;Xiaosong Gu ,&nbsp;Yan Liu","doi":"10.1016/j.mtbio.2025.101957","DOIUrl":"10.1016/j.mtbio.2025.101957","url":null,"abstract":"<div><div>Scar formation on skin wounds remains a considerable challenge in regenerative medicine. Various wound dressings, composed of biomaterials alone or in combination with bioactive factors, have been developed to improve healing outcomes. In this study, we designed a recombinant neurotrophin-3 (NT3) containing a silk fibroin light chain (SFL) and developed a silk fibroin (SF) hydrogel with NT3 activity. The SFL-NT3 protein bound to the heavy-light chains of SF and was efficiently integrated into the SF hydrogel. We evaluated the effect of the recombinant NT3-SFL hydrogel on wound healing in a mouse skin injury model. This hydrogel enhanced wound healing. Remarkably, SFL-NT3 increased the levels of type III collagen (Col3) during the healing process and induced hair follicle formation, which is a characteristic of scar-less healing. Further investigation revealed that SFL-NT3 upregulated Col3 expression in skin fibroblasts expressing the NT3 receptor, TrkC. NT3 activation of TrkC leads to Akt phosphorylation, resulting in elevated Sox2 levels, which in turn enhances Col3 transcription. Notably, TrkC inhibition abrogated the beneficial effects of SF + SFL-NT3 on wound healing, confirming its involvement in this signaling pathway. In conclusion, the SF hydrogel loaded with SFL-NT3 facilitated rapid and reduced scarring during wound healing, providing a promising approach for the clinical treatment of SF-based biomaterials that incorporate bioactive factors.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101957"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual testing methodology to predict the mechanical behavior of collagen hydrogels from nanoarchitecture","authors":"Elías Núñez-Ortega ,&nbsp;Pablo Blázquez-Carmona ,&nbsp;Raquel Ruiz-Mateos ,&nbsp;José E. Martín-Alfonso ,&nbsp;José A. Sanz-Herrera ,&nbsp;Esther Reina-Romo","doi":"10.1016/j.mtbio.2025.101962","DOIUrl":"10.1016/j.mtbio.2025.101962","url":null,"abstract":"<div><div>Collagen-based hydrogels are three-dimensional, cross-linked structures capable of mimicking the extracellular fibered matrix of biological tissues, making them particularly well-suited for biomedical applications. These hydrogels typically exhibit highly non-linear mechanical behavior, which strongly depends on their internal nanostructural characteristics - an interconnection that remains poorly understood. The aim of this work is to combine high resolution imaging with a multiscale <em>in silico</em> structural model to virtually reproduce the mechanical behavior of a widely used collagen-based hydrogel, using solely its nanoarchitecture as input. The real fiber structure of the hydrogel was originally quantified at the nanometer scale using state-of-the-art microscopy, specifically, focused ion beam-scanning electron microscopy (FIB-SEM). <em>In silico</em> shear tests were then performed on the reconstructed collagen matrix to compute, through a multiscale approach, its homogenized mechanical response, including the energies and stresses developed by the fibers during the tests. Different samples of the hydrogel were also mechanically characterized by means of rheological tests to fit the model and show the feasibility of the methodology. The <em>in silico</em> simulations successfully captured the detailed mechanical interactions between fibers as well as the experimental non-linear mechanical behavior of the hydrogels. Results also highlight the relevant role of the bending energy throughout the entire range of deformation analyzed. This methodology provides a framework to elucidate the structure-mechanical behavior relationship of fiber network topologies, and can be applied to predict mechanical response of both native tissues and biomaterials based exclusively on their fibered nanostructures.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101962"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural killer cell membrane manipulation for augmented immune synapse and anticancer efficacy","authors":"Minseon Ryu ,&nbsp;Eunha Kim ,&nbsp;Sungjun Kim,&nbsp;Kyobum Kim","doi":"10.1016/j.mtbio.2025.101965","DOIUrl":"10.1016/j.mtbio.2025.101965","url":null,"abstract":"<div><div>Natural killer (NK) cells are proving a powerful platform in cancer immunotherapy due to their innate cytotoxicity and ability to recognize tumor cells independently of antigen presentation. In preclinical and clinical studies, engineered NK cells expressing chimeric antigen receptors (CARs) have demonstrated strong antitumor efficacy, showcasing the potential of genetic reprogramming to enhance specificity and activation. In parallel, biomaterial-assisted surface engineering has gained momentum as a complementary strategy, offering a genome-independent and modular means of customizing NK cell functionality. Recent advances in covalent conjugation, metabolic glycoengineering, bio-orthogonal click chemistry, and hydrophobic insertion using biomaterials have facilitated the precise presentation of targeting ligands and immunomodulatory molecules directly onto the NK cell membrane. These strategies support programmable cell–tumor interactions, while maintaining the native cytotoxicity of NK cells. Although several challenges remain, including <em>in vivo</em> persistence and control of effector responses, surface engineering approaches offer practical advantages in flexibility, reversibility, and manufacturing. This review highlights key advances in NK cell-based cancer immunotherapy, with particular focus on: (1) the therapeutic potential and clinical application of native NK cells, (2) the development of CAR−NK cell platforms, and (3) emerging biomaterial-assisted surface engineering strategies to enhance immune synapse. Together, these developments expand the toolkit for NK cell-based therapies and suggest that material-guided engineering may play a valuable role alongside genetic strategies in shaping the next generation of cancer immunotherapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101965"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of nano mume Fructus charcoal by reshaping inflammatory microenvironment as biofunctional wound nanomedicine","authors":"Pan Liang ,&nbsp;Yining Ma ,&nbsp;Menglin Song ,&nbsp;Hong Wang ,&nbsp;Xin Peng ,&nbsp;Qin Sun ,&nbsp;Hongping Shen ,&nbsp;Zengjin Liu ,&nbsp;Pei Luo ,&nbsp;Wei Ren","doi":"10.1016/j.mtbio.2025.101971","DOIUrl":"10.1016/j.mtbio.2025.101971","url":null,"abstract":"<div><div>Hemorrhagic wound management in severe trauma patients remains a vital clinical problem because of uncontrolled bleeding and inflammatory responses. Charcoal drugs have shown great potential for facilitating hemostasis and wound repair for more than two thousand years. Nevertheless, studies on the inherent biological activities of charcoal drugs and their corresponding pharmacological effects are far from satisfactory. Herein, a novel carbon dot prepared from charred <em>Mume Fructus</em> (CMF-CDs) for enhancing hemorrhagic wound healing was reported for the first time. Surprisingly, the as-prepared CMF-CDs showed good biocompatibility, reactive oxygen species scavenging ability and protection of human cells from oxidative damage. Inspired by this, we confirmed that CMF-CDs enhanced hemorrhagic wound healing by facilitating hemostasis, M2-type macrophage polarization, angiogenesis, collagen deposition and tissue regeneration. Proteomic results further revealed that the CMF-CDs reshaped the inflammatory microenvironment of the wound by reducing excessive ROS produced by tissue oxidative phosphorylation and the tricarboxylic acid cycle metabolic pathway. Ultimately, the discovery of CMF-CDs holds enormous promise for managing severe traumatic wounds and provides theoretical and material insights into charcoal drug-mediated hemostasis applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101971"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-stretchable, adhesive and photothermal multifunctional hydrogel for renal denervation to treat hypertension","authors":"Heng Lin ,&nbsp;Haosong Xi ,&nbsp;Hengyang Liu ,&nbsp;Ze Zhao ,&nbsp;DangWei Li ,&nbsp;Shixin Wang ,&nbsp;Di Yang ,&nbsp;Jiaxing Huang ,&nbsp;Rui Li ,&nbsp;Jiaming Qiao ,&nbsp;Hong Jiang ,&nbsp;Lilei Yu ,&nbsp;Fan Cheng ,&nbsp;Hongbing Deng","doi":"10.1016/j.mtbio.2025.101966","DOIUrl":"10.1016/j.mtbio.2025.101966","url":null,"abstract":"<div><div>Hypertension is a major risk factors for stroke, cardiovascular diseases, and chronic kidney diseases. Although taking antihypertension drugs improves the prognosis of hypertensive patients to a certain extent, there are still lots of refractory hypertension and non-adherent patients. Therefore, it is imperative to explore more effective and targeted treatment approaches. Herein, we construct a multifunctional photothermal hydrogel to treat hypertension and cardiac remodeling through renal denervation (RDN) under 808 nm near-infrared (NIR) laser irradiation. The prepared multifunctional hydrogel, which is customized based on renal perivascular microenvironment, exhibits excellent stretchability, mechanical properties, flexibility and adhesion, ensuring it with remarkable conformability to the dynamic movements of the renal arteries throughout RDN procedures. Photothermal RDN achieved significant blood pressure reduction, with sustained decreases of 13.3 ± 2.5 mmHg in systolic blood pressure and 10.6 ± 1.2 mmHg in diastolic blood pressure, persisting for 8 weeks post-treatment. Additionally, a reduction in myocardial fibrosis and cardiomyocyte hypertrophy is observed, which indicative of the alleviation of cardiac remodeling. The antihypertensive effect of photothermal RDN is mediated through inhibition of the renin-angiotensin-aldosterone system (RAAS) pathway and activation of the cGMP-PKG signaling cascade. The photothermal hydrogel-mediated RDN treatment provides a promising and innovative strategy for treating hypertension and cardiac remodeling.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101966"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term SiNPs exposure induced genetic instability and malignant transformation via SQSTM1/p62-mediated autophagy dysfunction in lungs","authors":"Kanglin Yan ,&nbsp;Yuexiao Ma ,&nbsp;Xuemin Shi ,&nbsp;Chen Liang ,&nbsp;Ruiyang Ding ,&nbsp;Zhiwei Sun ,&nbsp;Junchao Duan","doi":"10.1016/j.mtbio.2025.101972","DOIUrl":"10.1016/j.mtbio.2025.101972","url":null,"abstract":"<div><div>As one of the most widely used nanomaterials, silica nanoparticles (SiNPs) have raised significant concerns regarding their toxicity, while their potential carcinogenicity remains poorly understood. During occupational exposure, SiNPs primarily enter the human body through the respiratory tract, thus we aimed to investigate the SiNPs-induced malignant transformation and correlated mechanisms in lungs. Fischer 344 rats underwent weekly intratracheal instillation of SiNPs for six months, followed by an additional six-month observation period to evaluate long-term effects. Results demonstrated the development of precancerous lesions in lungs of rats, which were associated with increased pulmonary glucose metabolism, chronic inflammation, squamous metaplasia, and epithelial-mesenchymal transition (EMT). Similarly, BEAS-2B cells exposed to SiNPs over 40 passages exhibited enhanced abilities in proliferation, migration, invasion, and anchorage-independent colony formation. In addition, genotoxicity was observed in BEAS-2B cells, including increased micronucleus formation, aberrant cell division, and elevated chromosomal aberration frequency. Mechanistically, SiNPs activated SQSTM1/p62-mediated autophagy dysfunction, which in turn induced mitotic catastrophe by interfering with the MDM2/p53/Aurora B signaling pathway. Concurrently, SQSTM1/p62 accumulation suppressed DNA damage repair by enhancing its interaction with RNF168. Molecular docking simulation further predicted that SiNPs directly bind to SQSTM1/p62 through electrostatic interactions, inducing conformational changes in SQSTM1/p62. Notably, SQSTM1/p62-knockout significantly attenuated SiNPs-induced DNA damage and malignant transformation , and modulated the expression of Aurora B and RNF168 signaling pathways. These findings demonstrated the critical role of SQSTM1/p62-mediated autophagy dysfunction in SiNPs-induced genotoxicity and malignant transformation in lungs, offering novel insights into SiNPs-related carcinogenicity.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101972"},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization of engineered bacteria enhanced lipid nanomedicine accumulation in tumors for sonodynamic immunotherapy","authors":"Ying Xu ,&nbsp;Xiaojun Cai ,&nbsp;Jianrong Wu ,&nbsp;Yuanyi Zheng ,&nbsp;Yaotai Wang","doi":"10.1016/j.mtbio.2025.101943","DOIUrl":"10.1016/j.mtbio.2025.101943","url":null,"abstract":"<div><div>Lipid nanoparticles (LNPs) demonstrate liver-accumalating properties, enhancing drug delivery to hepatic tissues. This localized enrichment reduces systemic toxicity and mitigates drug resistance. However, critical limitations such as off-target effects, rapid clearance, and poor specificity hinder their clinical translation. Inspired by the tumor-targeting ability of bacteria, we constructed a multifunctional engineered bacteria system combining LNPs with probiotics by electrostatic adsorption. Specifically, the surface of <em>Bifidobacterium longum</em> (BL) was modified with sorafenib-loaded LNPs (designated PSI LNPs), enabling precise delivery of lipid nanomedicines to tumor sites. Meanwhile, ultrasound irradiation facilitated drug release and improved efficacy. On the one hand, we used fluorescence imaging to observe and monitor live-engineered bacteria. Compared to the single PSI LNPs group, the tumor accumulation of lipid nanomedicines loaded with engineered bacteria exhibited a 66 % increase. The results demonstrated that the engineered bacteria not only augmented the accumulation of nanomedicines but also prolonged the retention time for a sustained effect on the tumor. On the other hand, we further explored synergistic immune system activation by <em>Bifidobacterium</em> and sonodynamic therapy, which stimulated dendritic cell maturation, T cell infiltration, and CD8⁺ T cell activation. In conclusion, this delivery strategy of engineered bacteria targeting the tumor microenvironment is expected to overcome the limitations of conventional LNPs and provide a promising approach for treating hepatocellular carcinoma.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101943"},"PeriodicalIF":8.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}