Materials Today Bio最新文献

{"title":"All-in-one nerve guidance: transplanting a gradient scaffold with immobilized Schwann cells for peripheral nerve regeneration.","authors":"Shengwen Zhu, Rui Cui, Shuai Qiu, Xi Zhang, Jingxin Ma, Wan Duan, Peiyao Li, Daping Quan, Zehong Yang, Sien Zhang, Zilong Rao, Ying Bai","doi":"10.1016/j.mtbio.2026.103294","DOIUrl":"10.1016/j.mtbio.2026.103294","url":null,"abstract":"<p><p>Peripheral nerve injury (PNI), especially those with long-distance transected defects, remains a major clinical challenge due to limited regenerative capacity associated with inadequate endogenous Schwann cell (SC) support. Here, we developed a biomimetic nerve graft with a defined spatial gradient of immobilized SCs to facilitate effective transplantation and axonal guidance, thus enhancing peripheral nerve regeneration. SCs were initially encapsulated within decellularized nerve matrix (DNM) microgels using a customized flow-focusing microfluidic device. The DNM microgels supported good viability, facilitated cellular proliferation, and preserved the repair phenotype of the encapsulated SCs, while maintaining their advantageous paracrine activity conducive to axon extension. Furthermore, these microgels were incorporated into the density-gradient pores of a pre-designed scaffold, resulting in a gradient SC-laden scaffold, designated as MGs@G-scaffold. Notably, the spatially graded SCs within the MGs@G-scaffold created a stable environmental gradient of neurotrophic factors, thereby providing sustained biochemical cues to guide axonal elongation. Finally, when transplanted into a 15-mm rat sciatic nerve defect model, the SC-laden scaffolds exhibited significantly improved axonal regeneration, remyelination, and functional recovery compared with the SC-free scaffolds. Additionally, MGs@G-scaffolds outperformed scaffolds with uniform SC distribution (MGs@H-scaffolds), achieving therapeutic outcomes comparable to autografts. Overall, this study demonstrates that transplanting spatially graded SCs embedded in a tissue-engineered bioactive scaffold offers sustained ongoing support for cells, gradient biochemical signals, and a pro-regenerative microenvironment for nerve regeneration and functional recovery, which holds great promise for long-distance PNI repair.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103294"},"PeriodicalIF":11.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS-responsive nanocarriers for the delivery of atorvastatin in ischemic MCAO rats promoting neuroprotection.","authors":"Raveena Nagareddy, Aravindkumar Sundaram, Ja-Hae Kim, Ji Hye Kim, Shyam Vasvani, Seho Kweon, In-Kyu Park, Yong Yeon Jeong, Kang-Ho Choi","doi":"10.1016/j.mtbio.2026.103296","DOIUrl":"10.1016/j.mtbio.2026.103296","url":null,"abstract":"<p><p>Ischemic stroke remains a major global public health burden, ranking among the leading causes of long-term neurological disability and mortality. Despite advances in acute care, drug delivery to injured brain tissue is hindered by the poor permeability of the blood-brain barrier (BBB), a challenge exacerbated by excess reactive oxygen species (ROS), particularly hydrogen peroxide (H₂O₂), produced during ischemia-reperfusion. This oxidative surge also offers an opportunity to design ROS-responsive, site-specific therapeutic delivery systems. Therefore, this study aims to develop PEGylated thioketal-based nanocarriers (PTC) loaded with atorvastatin, a statin with both neuroprotective and anti-inflammatory properties. The thioketal linker in PTC undergoes selective cleavage by H₂O₂, enabling the nanocarrier to function as both a smart drug delivery vehicle and an ROS scavenger. <i>In vitro</i> experiments using oxygen-glucose deprivation-treated BV-2 microglial cells demonstrated that PTC-statin significantly lowered intracellular ROS, inhibited lipid peroxidation, and reduced inflammatory cytokine release. <i>In vivo</i>, intravenous PTC-statin administration in a rat middle cerebral artery occlusion model significantly reduced infarct size, improved motor coordination (<i>p</i> = 0.032), and enhanced neurological outcomes (<i>p</i> = 0.002), with MRI confirming infarct reduction as early as day 5 (<i>p</i> = 0.02). Molecular and histological analyses further revealed restored BBB integrity, reduced neuronal apoptosis, and promoted neurogenesis. Overall, these findings highlight PTC-statin as a precision nanomedicine that enables site-specific, ROS-triggered drug release and mitigates oxidative damage, offering a targeted therapeutic strategy for ischemic stroke recovery.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103296"},"PeriodicalIF":11.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13242022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrahedral framework nucleic acids carrying tranexamic acid to alleviate ultraviolet B-induced skin pigmentation.","authors":"Tao Cai, Junyang Huang, Li Chen, Yi Lin, Kaiqun Liu, Yuanyuan Deng, Jiajin Feng, You Wang, Xiaoyan Ding","doi":"10.1016/j.mtbio.2026.103287","DOIUrl":"10.1016/j.mtbio.2026.103287","url":null,"abstract":"<p><p>Hyperpigmentation disorders such as melasma require topical therapies that are both effective and well tolerated, yet many current depigmenting agents are limited by irritation or inconsistent efficacy. Tranexamic acid (TXA) is a clinically used small molecule with emerging anti-pigmentation utility, but its topical performance is often constrained by insufficient penetration and limited cellular access. In this work, we engineered a tetrahedral framework nucleic acid (tFNA)-enabled TXA formulation (tFNAs-TXA) to enhance topical delivery and anti-melanogenic activity. tFNAs-TXA was successfully constructed with preserved nanostructure, physicochemical stability, and efficient cellular internalization. Functionally, tFNAs-TXA significantly attenuated α-MSH-induced melanogenesis in B16 cells without overt cytotoxicity and downregulated the cAMP-p-CREB-MITF-TYR signaling cascade at both protein and mRNA levels. In a UVB-induced mouse model, topical tFNAs-TXA markedly reduced dorsal pigmentation and mitigated UVB-associated histopathological changes, including melanin deposition, collagen disorganization, and epidermal hyperplasia, achieving depigmenting efficacy with better gross tolerability. Together, these findings establish tFNAs-TXA as a concise, non-invasive delivery strategy that potentiates TXA for safer and more effective management of UV-related hyperpigmentation.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103287"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13254978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual activation of cuproptosis and excessive autophagy by copper-bismuth metal-organic frameworks-loaded detachable microneedles in oral leukoplakia.","authors":"Keyi Zhou, Meihua Gao, Yuqi Wan, Beibei Cong, Tingting Zhu, Zhou Ye, Tao Zhang, Wanchun Wang","doi":"10.1016/j.mtbio.2026.103283","DOIUrl":"10.1016/j.mtbio.2026.103283","url":null,"abstract":"<p><p>Oral leukoplakia (OLK), a prevalent precancerous lesion with considerable malignant transformation potential, remains a major clinical challenge due to the lack of effective and minimally invasive therapies. Here, we developed novel copper-bismuth metal-organic frameworks (CBM) and incorporated them into a bilayer detachable microneedle system (CBMMN) to achieve localized and sustained delivery. CBM markedly downregulated pyruvate dehydrogenase kinase 3 (PDK3), thereby orchestrating the cooperative activation of cuproptosis and excessive autophagy, which synergistically suppressed the viability of dysplastic oral keratinocytes. In vivo studies further demonstrated that CBMMNs effectively eliminated OLK with minimal systemic toxicity, underscoring their therapeutic efficacy and biosafety. By combining cuproptosis and excessive autophagy, CBMMNs establish a mechanistic foundation distinct from conventional approaches and offer a novel therapeutic approach for OLK management.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103283"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13267564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L.","authors":"Hao Yin, Teng-Fei Wan, Shan-Shan Rao, Ze-Hui He, Zheng-Zhao Liu, Zhen-Xing Wang, Zhi-Rou Lin, Yi-Yi Wang, Ling Jin, Yi-Wei Liu, Guo-Qiang Zhu, Jiang-Shan Gong, Jia Cao, Zun Wang, Yi-Juan Tan, Yu-Xuan Qian, Xin Wang, Qin-Lu Lin, Ying Liang, Chun-Yuan Chen, Hui Xie","doi":"10.1016/j.mtbio.2026.103289","DOIUrl":"10.1016/j.mtbio.2026.103289","url":null,"abstract":"<p><p>Osteoporosis is frequently accompanied by a shift in the fate of bone marrow mesenchymal stromal cells (BMSCs) from osteogenesis toward adipogenesis, contributing to bone-fat imbalance and fragility fractures. Here, we evaluated a naturally occurring eight-amino acid rice bran-derived peptide, RBAP (KHNRGDEF), together with an aptamer-RBAP conjugate designed to improve bone accumulation via a previously identified BMSC-targeting nucleic acid aptamer. In ovariectomized mice, RBAP administered either preventively (early after ovariectomy) or therapeutically (after osteoporotic phenotypes had developed) preserved trabecular microarchitecture, improved mechanical properties, reduced marrow adiposity, and accelerated osteoporotic fracture healing. RBAP also conferred bone-protective effects in models of natural aging and disuse osteoporosis. <i>In vitro</i>, RBAP promoted osteogenic differentiation and suppressed adipogenic differentiation of primary BMSCs without detectably affecting osteoclastogenesis. Proteomic pull-down and functional screening identified eukaryotic translation initiation factor 3 subunit L (eIF3L) as a key RBAP-interacting protein, and genetic suppression of <i>eIF3l</i> attenuated RBAP-induced osteogenesis and blunted its bone protective capacity <i>in vivo</i>. Conjugation of RBAP to the BMSC-targeting aptamer increased BMSC uptake and bone tissue accumulation, thereby enhancing its pro-osteogenic and anti-osteoporotic effects at the same administered dose. Our study identifies RBAP as a bioactive peptide candidate for correcting bone-fat imbalance in osteoporosis and support aptamer conjugation as a modular strategy to improve bone delivery.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103289"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13255075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicle-embedded alginate hydrogel patch for accelerated wound healing.","authors":"Won Ho Jang, Van Dat Bui, Van Hieu Duong, Sol Shin, Jungmi Lee, Torsha Ghosh, Chang Hyun Lee, Soyoung Son, Jiyeon Kim, Eun-Cheol Lee, Suk Ho Bhang, Jae Hyung Park","doi":"10.1016/j.mtbio.2026.103288","DOIUrl":"10.1016/j.mtbio.2026.103288","url":null,"abstract":"<p><p>The process of skin wound healing is markedly impaired by elevated levels of reactive oxygen species (ROS) and the persistent presence of pro-inflammatory macrophages in the injured tissue. To address these pathological challenges, we herein developed an alginate-based hydrogel patch (EGEV-Gel) engineered for the sustained release of human adipose stem cell-derived extracellular vesicles (hASC-EVs) and epigallocatechin gallate (EGCG). hASC-EVs were incorporated to promote tissue regeneration, while EGCG served as a potent ROS scavenger. In an <i>in vitro</i> wound healing model using the transwell insert system, EGEV-Gel demonstrated remarkable efficacy in suppressing M1 macrophage polarization, scavenging excessive ROS, and restoring the functional capacity of dermal fibroblasts and endothelial cells compromised by oxidative stress. When applied to the acute wound-induced mouse, EGEV-Gel significantly reduced the M1 macrophage population and the ROS level at 3 days post-treatment. Histological analysis of the skin layers further confirmed restoration of skin architecture, as demonstrated by the normalized thickness of key structural components, including the epidermis, collagen layer, and granulation tissue. These findings highlight the therapeutic potential of the hASC-EVs/EGCG-loaded hydrogel patch as a promising platform for promoting wound repair and modulating the inflammatory microenvironment in cutaneous injuries.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103288"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DPEP1-Binding and mitochondria-targeted nanocomposite relieves acute respiratory distress syndrome by inhibiting Drp1-mediated mitochondria fission.","authors":"Ming He, Peng Zhang, Suya Bao, Rui Luo, Li Chen, Wenqi Huang, Weixi Jiang, An Zhang","doi":"10.1016/j.mtbio.2026.103291","DOIUrl":"10.1016/j.mtbio.2026.103291","url":null,"abstract":"<p><p>Acute respiratory distress syndrome (ARDS), a severe condition associated with high mortality, is characterized by uncontrollable inflammation and oxidative stress linked to mitochondrial dysfunction. Dynamin-related protein 1 (Drp1) drives pathological mitochondrial fission in patients with ARDS, leading to a sustained inflammatory response and excessive mitochondrial reactive oxygen species (mtROS) production. However, the specific inhibition of Drp1 in lung mitochondria remains challenging. Here, a multifunctional nanocomposite (DTP-LSA@MTC NPs) was developed by integrating the Drp1 inhibitor Mdivi-1 with a mitochondria-targeting tannic acid-cerium (TA-Ce) nanozyme network. Additionally, surface functionalization with an LSA peptide enabled specific binding to DPEP1 on the inflamed pulmonary endothelium, enhancing site-specific accumulation and competitively inhibiting neutrophil recruitment. Following intravenous administration, these nanoparticles efficiently targeted both pulmonary microvascular endothelial cells and mitochondria, suppressed the activity of the Drp1-NLRP3 inflammasome axis, and scavenged ROS, ultimately preventing the development of cytokine storms in preclinical models of ARDS. This targeted nanotherapeutic strategy offers a potent and translatable approach for treating ARDS and related inflammatory disorders.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103291"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design of a V-shaped DNA-targeted photosensitizer enables endogenous DNA damage-driven cGAS-STING activation and systemic antitumor immunity.","authors":"Yi Cai, Cai-Yun Wang, Min Dong, Zhang Lin, Cai-Qiang Liao, Ze-Jun Li, Tian-Yi Qin, Zhao-Hui Wang, Ya-Long Wang, Ming-Qiang Zhu","doi":"10.1016/j.mtbio.2026.103290","DOIUrl":"10.1016/j.mtbio.2026.103290","url":null,"abstract":"<p><p>The cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway is a central driver of antitumor immunity, yet its safe and efficient activation in solid tumors remains challenging. Here we report a V-shaped photosensitizer, 2C6, that functions as an in situ DNA-fragmentation agent by directly targeting double-stranded DNA (dsDNA). Owing to its A-π-D-π-A molecular framework, 2C6 exhibits efficient reactive oxygen species (ROS) generation under low-power white-light irradiation. Photoactivation of 2C6 induces concurrent mitochondrial and nuclear DNA damage, leading to the accumulation of oxidized dsDNA fragments in the cytosol, which is closely associated with activation of the cGAS-STING signaling pathway. In parallel, 2C6-PDT induces pyroptosis and immunogenic cell death (ICD), as evidenced by elevated levels of pyroptosis-associated markers (including NLRP3, cleaved caspase-1, and GSDMD), along with features of ICD such as CRT exposure, ATP secretion, and HMGB1 release. In a murine 4T1 triple-negative breast cancer (TNBC) postsurgical residual model, a single intraoperative 2C6-PDT prevented local tumor recurrence, established immune memory within the observation period, and suppressed distant tumor growth and lung metastasis without immunoadjuvants. Collectively, 2C6 couples photoinduced DNA damage to cytosolic dsDNA sensing, type I interferon signaling, and adaptive immune activation through a defined cascade encompassing DNA damage, dsDNA accumulation, cGAS-STING engagement, inflammatory signaling, and immune-cell infiltration. Together, these findings demonstrate that rationally engineered DNA-targeted photosensitizers can convert localized photodynamic tumor ablation into a systemic photoimmunotherapeutic response, providing a strategy to transform immunologically \"cold\" tumors into immune-responsive states.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103290"},"PeriodicalIF":11.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13242031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apical-out airway organoids as a pathophysiological model to study macrophage-mediated epithelial responses to microplastics.","authors":"Seon Young Choi, Chae Hwa Kim, Soyoung Hwang, Hyun Joo Kim, Seunghyun Bang, Jin Woo Lee, Da Hae Lee, Seungwon Ryu, Jooyeon Jung, Tae Hee Kim, Kuk Hui Son","doi":"10.1016/j.mtbio.2026.103278","DOIUrl":"10.1016/j.mtbio.2026.103278","url":null,"abstract":"<p><p>Respiratory exposure to airborne microplastics is increasingly implicated in airway inflammation, but human-relevant <i>in vitro</i> systems that separate direct epithelial particle contact from immune-cell-mediated paracrine effects remain limited. Here, we generated human airway basal stem cells (hABSCs)-derived apical-out airway organoids (AOAOs) with outward-facing ciliated surfaces and used them to evaluate operationally size-fractionated, polydisperse polystyrene microplastic (PS-MP) fractions. PS-MPs were characterized by laser diffraction, SEM, FT-IR, Nile red labeling, endotoxin testing, and culture-media stability/protein-adsorption assays. AOAOs were exposed under two submerged configurations: direct application of PS-MPs to the organoid surface or indirect exposure to conditioned media from PS-MP-treated THP-1-derived macrophages. CCK-8, MTT, and LDH range-finding assays identified 1 and 100 μg/mL as non-cytotoxic nominal submerged doses for macrophage-organoid experiments. Direct exposure of AOAOs to small, medium, or large PS-MP fractions did not induce marked TNF-α expression, mucin secretion, epithelial barrier disruption, or goblet cell hyperplasia. In contrast, the small PS-MP fraction showed the strongest macrophage association and induced extracellular IL-1β release; conditioned media from small PS-MP-exposed macrophages increased TNF-α expression and Muc5ac-positive goblet cell populations in AOAOs. Selective caspase-1 activation in the small 100 μg/mL condition, together with cleaved GSDMD immunoreactivity and LDC7559-sensitive IL-1β release, supported a caspase-1-associated, GSDMD-sensitive component of IL-1β secretion, but did not establish definitive canonical inflammasome activation. This AOAO platform provides a submerged mechanistic proof-of-concept for dissecting macrophage-conditioned airway epithelial remodeling by PS-MPs, while not representing aerosol deposition or inhalation-equivalent dosimetry.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103278"},"PeriodicalIF":11.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced hemostasis and bone regeneration achieved by thrombin-simvastatin-polydopamine loaded spray-dried β-TCP/ gelatin/ soy protein isolate biocomposites in critical rat calvarial defects.","authors":"Nusrat Jahan, Prayas Chakma Shanto, Myeongki Park, Md Abdullah Al Fahad, Hai-Doo Kim, Byong-Taek Lee","doi":"10.1016/j.mtbio.2026.103275","DOIUrl":"10.1016/j.mtbio.2026.103275","url":null,"abstract":"<p><p>Effective bone tissue engineering strategies require scaffolds with tailored properties to address specific bone abnormalities and fractures. Herein, porous biocomposite scaffolds were fabricated via repeated freeze-casting and three-layer coating. The prepared system consisted of spray-dried β-TCP granules and bio-macromolecule gelatin-based soya protein isolate (S) combined with a layer of the biomimetic adherent poly-dopamine (PDA) for simvastatin (SIM) coating via dopamine self-polymerization through the dip-coating process. Thrombin (Th) was then loaded to make the final hemostatic hybrid bone graft (TGS/SIM/Th). The PDA adhered layer improved the mechanical qualities as expected (13.93 MPa). Additionally, MC3T3-E1 cell migration (>90%) and osteogenic differentiation (∗∗∗p < 0.001) were superior in vitro. The sustained release of SIM (∼78%) can help form new capillary networks, and the burst release of Th (∼97.2%) supports the creation of stable blood coagulation. The hemostatic time of the TGS/SIM/Th significantly decreased (∗∗∗∗p < 0.0001) in the critical-sized rat calvarial defect model and tail amputation model, which would effectively enhance the bone repair. This study shows that the fabricated biocomposite could represent a new approach for treating bone defects while absorbing a large amount of blood from onsite bleeding.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103275"},"PeriodicalIF":11.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13253149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}