Materials Today Bio最新文献

{"title":"A pathogen peptidoglycan scaffold coated with artificial biomembrane promotes broad resistance to bacterial infections by dynamically reprogramming macrophage metabolism","authors":"Junjie Guo ,&nbsp;Shuo Jia ,&nbsp;Zibo Mai ,&nbsp;Chaonan Wang ,&nbsp;Zheng Jia ,&nbsp;Jiaqing Wang ,&nbsp;Xinran Yao ,&nbsp;Jiaqi Liu ,&nbsp;Fang Wang ,&nbsp;Junwei Ge","doi":"10.1016/j.mtbio.2025.102266","DOIUrl":"10.1016/j.mtbio.2025.102266","url":null,"abstract":"<div><div>The increasing severity of multidrug-resistant (MDR) bacteria and the shortage of effective treatment strategies urgently require the development of new immunotherapies to combat superbug infections. Trained immunity may offer a novel and effective mechanism to combat resistant superbugs. However, there are currently few materials capable of effectively activating trained immunity, highlighting the need for new agents that provide more durable protection. In this study, we developed a bacterium-like particle (BLP) based on protein-free artificial biomembrane coating immune activator, named LM@pBLP, which features a simple and rapid preparation process, excellent biocompatibility, long-term stability, and a cost-effective advantage. LM@pBLP trains the immune system to target a broad range of pathogens, offering rapid, broad-spectrum, and long-lasting protection against MDR infections. After stimulation with LM@pBLP, it activates glutathione metabolism and amino acid metabolism, induces macrophage metabolic and epigenetic reprogramming changes, and regulates phagocytosis and inflammatory responses to infection. Additionally, LM@pBLP regulates reactive oxygen species (ROS), thereby maintaining oxidative stress homeostasis. Our study demonstrates that LM@pBLP primarily provides rapid, broad-spectrum, and long-lasting protection for experimental animals by activating trained immunity, which opens a new avenue for addressing MDR infections.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102266"},"PeriodicalIF":10.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels loaded with aqueous supramolecular fullerene complex for the promotion of diabetic chronic wound healing","authors":"Yue Wang ,&nbsp;Hui Fang ,&nbsp;Guofeng Zhou ,&nbsp;Guanzhao Zhang ,&nbsp;Xiao Wang ,&nbsp;Zhiyong Zhang ,&nbsp;Yingjie Zhao ,&nbsp;Bo Li","doi":"10.1016/j.mtbio.2025.102250","DOIUrl":"10.1016/j.mtbio.2025.102250","url":null,"abstract":"<div><div>Impaired chronic wound healing in diabetes remains a significant clinical problem, with excessive oxidative stress being a major cause of the delay. In this study, we developed a water-soluble 6HPB@C₆₀ complex by encapsulating fullerene C₆₀ molecules within a cubic supramolecular cage 6HPB. Subsequently, 6HPB@C₆₀ was loaded in the sodium alginate hydrogel to create an antioxidant system (6HPB@C₆₀ gel) for diabetic wound healing. 6HPB@C₆₀ gel exhibits a homogeneous and interconnected porous microstructure, demonstrating excellent tissue adhesion, low cytotoxicity, and excellent antioxidant capacity. Diabetic chronic wounds heal faster with 6HPB@C₆₀ gel, which eliminates reactive oxygen species continuously. By regulating macrophage polarization, pro-inflammatory states are reduced, while at the same time cell proliferation, epithelialization, and collagen deposition and alignment are also facilitated. In conclusion, a possible solution for chronic wound management can be found in the 6HPB@C₆₀ gel, which works significantly to accelerate diabetic wound closure and improve wound quality.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102250"},"PeriodicalIF":10.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of purple sweet potato-derived selenium nanoparticles accelerates wound healing through pyroptosis regulation","authors":"Chen Chen ,&nbsp;Fructueux Modeste Amona ,&nbsp;Ziqi Sha ,&nbsp;Jiamin Li ,&nbsp;Yongding Ke ,&nbsp;Yuxin You ,&nbsp;Luyuan Yang ,&nbsp;Guangfu Liao ,&nbsp;Xi Chen ,&nbsp;Yipeng Pang ,&nbsp;Yi Liu","doi":"10.1016/j.mtbio.2025.102269","DOIUrl":"10.1016/j.mtbio.2025.102269","url":null,"abstract":"<div><div>Although advances in nanomedicine using nanoparticles (NPs) derived from natural compounds have provided us with much insight into how to recover from wound infections, exploring the wound healing pathway remains a new perspective that has attracted significant interest in addressing wound complications challenges. Here, we harnessed the therapeutic potential of novel selenium nanoparticles (SeNPs) derived from Purple <em>sweet potato</em> (PSp) extracts to promote wound healing through the regulation of pyroptosis-related pathways. PSp-SeNPs, with an average particle size of 80–100 nm, demonstrated significant antibacterial activity against <em>S. aureus</em> and MRSA clinical pathogens. The mechanism involves impairment of bacterial growth, biofilm formation, and metabolic processes through ATP depletion. Moreover, PSp-SeNPs impairs NLRP3-mediated pyroptosis including p-IκBα, p-NF-κB, IL-18, and IL-1β. This regulatory effect decreases inflammatory cytokines IL-6 and TNF-α while promoting angiogenesis and collagen formation through increased expression levels of TGF-β, VEGFA, and CD31, thus accelerating wound healing. <em>In vivo</em> assessments confirmed that PSp-SeNPs significantly enhanced wound healing without adverse effects, indicating their high biocompatibility and bioavailability. This groundbreaking study elucidates the therapeutic potential of PSp-based selenium nanoparticles, facilitating the development of precise and efficient treatment strategies for wound healing and diverse medical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102269"},"PeriodicalIF":10.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-homing exosomes enable targeted delivery of siRNA and isoimperatorin for overcoming BTK inhibitor resistance in DLBCL","authors":"Ruowen Sun ,&nbsp;Yanchao Yang ,&nbsp;Bin Zhang ,&nbsp;Jiayuan Chen ,&nbsp;Ye Wang ,&nbsp;Zehui Jiang ,&nbsp;Linlin Zhang ,&nbsp;Milad Ashrafizadeh ,&nbsp;Gautam Sethi ,&nbsp;Jing Shen ,&nbsp;Zuofei Chi","doi":"10.1016/j.mtbio.2025.102267","DOIUrl":"10.1016/j.mtbio.2025.102267","url":null,"abstract":"<div><div>Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma, but over one-third of patients relapse or develop refractory disease after first-line therapy. Novel therapeutic strategies are required to address persistent unmet clinical needs for DLBCL. This study aimed to develop an exosome-based drug delivery system for the targeted combination therapy of siRNA against Bruton's tyrosine kinase (BTK, an established therapeutic target in B cell lymphomas) and isoimperatorin (ISOIM, an active natural furanocoumarin showing anti-tumor effects) in DLBCL. Tumor exosomes were isolated as the delivery carrier. ISOIM/siBTK@Exosome was prepared by encapsulating ISOIM and si-BTK into exosome using electroporation. Cellular uptake, immune escape, targeted delivery efficiency, anti-lymphoma activity and biosafety of ISOIM/siBTK@Exosome were evaluated in two DLBCL cell lines and in tumor-bearing mice. ISOIM/siBTK@Exosome displayed significant anti-lymphoma activity compared to ISOIM@Exosome or siBTK@Exosome alone, demonstrating synergistic therapeutic role of ISOIM and si-BTK. Besides, ISOIM/siBTK@Exosome can accelerate T cells activation and prevent macrophage M2 polarization in vitro. Administration of ISOIM/siBTK@Exosome to tumor-bearing mice significantly inhibited tumor growth and prolonged survival. The ISOIM/siBTK@Exosome was biocompatible and biosafe in vivo without damage on the major organs in H&amp;E staining. The prepared ISOIM/siBTK@Exosome may provide novel targeted therapeutic strategy to be applied in the clinical management of patients with DLBCL.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102267"},"PeriodicalIF":10.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrier-free nanoparticles based on natural products trigger dual \"synergy and attenuation\" for enhanced phototherapy of liver cancer","authors":"Xiongqin Wang ,&nbsp;Kunhui Sun ,&nbsp;Junlin Dong ,&nbsp;Yanli Ge ,&nbsp;Haifan Liu ,&nbsp;Xingyue Jin ,&nbsp;Xiao Pei ,&nbsp;Ping Wang ,&nbsp;Jianbing Jiang ,&nbsp;Tiejie Wang ,&nbsp;Xuelei Hu ,&nbsp;Shuguang Yuan ,&nbsp;Bing Wang ,&nbsp;Xie-an Yu","doi":"10.1016/j.mtbio.2025.102278","DOIUrl":"10.1016/j.mtbio.2025.102278","url":null,"abstract":"<div><div>Carrier-free nanoparticles have attracted significant interest owing to their exceptional drug-loading efficiency, simple preparation, and good biosafety. Inspired by the synergism and attenuation strategy of natural products, carrier-free nanoparticles were constructed from a compatibility perspective, aiming to carry natural products to tumor sites as nanoparticles to achieve precise anti-cancer effects. Herein, the carrier-free nanoparticles based on gambogic acid and glycyrrhizic acid (GG NPs) were successfully formed via self-assembly. The internally generated \"synergism and attenuation\" GG NPs could enhance the anti-tumor effect, reduce toxicity to normal cells, and exhibit tumor-targeting capabilities. Further, to explore the role of GG NPs in enhancing phototherapy, the photosensitizer ZnPc 2 was self-assembled with GG NPs to form GGZ NPs. <em>In vitro</em> studies demonstrated that the GGZ NPs significantly improved phototherapecutic efficacy. Compared with ZnPc 2, the photothermal conversion efficiency was increased to 80.8%, along with a 1.8-fold increase in tumor growth inhibition, and the singlet oxygen yield of GGZ NPs increased by 2-fold. <em>In vivo</em> experiments demonstrated that GGZ NPs exhibit significant liver-targeting ability and could reduce the liver toxicity induced by gambogic acid, as well as decrease the blood biochemical levels of AST and ALT, achieving externally assisted \"synergism and attenuation\". Hence, carrier-free nanoparticles based on natural products would bring a new treatment modality to the field of precision liver tumor therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102278"},"PeriodicalIF":10.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced bifunctional nanotherapeutics display multi-tiered defense against Magnaporthe oryzae through targeted fungal inhibition and rice immunity enhancement","authors":"Mohammad Shafiqul Islam ,&nbsp;Muhammad Noman ,&nbsp;Zhen Zhang ,&nbsp;Temoor Ahmed ,&nbsp;Yingying Cai ,&nbsp;Jing Wang ,&nbsp;Md. Arshad Ali ,&nbsp;Rahila Hafeez ,&nbsp;Haiping Qiu ,&nbsp;Zhongna Hao ,&nbsp;Rongyao Chai ,&nbsp;Munazza Ijaz ,&nbsp;Yanli Wang ,&nbsp;Bin Li ,&nbsp;Jiaoyu Wang","doi":"10.1016/j.mtbio.2025.102275","DOIUrl":"10.1016/j.mtbio.2025.102275","url":null,"abstract":"<div><div>Rice blast disease, caused by <em>Magnaporthe oryzae</em> (<em>Mo</em>), severely threatens global rice production. In this study, biogenic copper nanoparticles (bio-CuNPs) were synthesized extracellularly using <em>Bacillus amyloliquefaciens</em> Q1 and characterized for antifungal activity and plant defense induction. Bio-CuNPs (16–62 nm, average 37 nm) exhibited potent antifungal effects by significantly inhibiting <em>Mo</em> mycelial growth, conidial germination, and appressorium formation in a dose-dependent manner. Microscopic observations revealed that bio-CuNPs disrupted <em>Mo</em> hyphal integrity, caused intracellular leakage, and induced DNA damage. Transcriptomic profiling identified key regulatory genes in <em>Mo</em> upon CuNP exposure, with key disruptions in cell wall biosynthesis (<em>MoCHS-A</em>, <em>MoCHS-B</em>, <em>MoCHS-C</em>, and <em>MoCHS-D</em>) and membrane transport pathways (<em>MoMFS-1 MoMFS-2</em>, and <em>MoMSC-2</em>). Deletion mutants for these genes demonstrated heightened sensitivity to bio-CuNPs, indicating that these genes are critical for helping <em>Mo</em> to withstand the antifungal effects of bio-CuNPs. However, bio-CuNPs disrupted their functions in <em>Mo</em>, confirming these genes as one of the molecular targets to suppress <em>Mo</em> growth and virulence. <em>In planta</em> assays revealed that bio-CuNP foliar application reduced disease severity, improved plant growth, and activated antioxidant enzymes, while suppressing oxidative stressors (<em>i.e.</em>, super oxide radicle and hygrogen peroxide). Metabolomic analysis revealed significant alterations in defense-related pathways, including phenylpropanoid and amino acid metabolism. Additionally, CuNPs enhanced salicylic acid and methyl jasmonate levels, which subsequently upregulated defense gene expression. Cytotoxicity assays revealed that bio-CuNPs were non-toxic to AML12 cells at effective concentrations, highlighting their potential as an eco-friendly strategy for sustainable rice blast management.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102275"},"PeriodicalIF":10.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive recombinant human elastin gel for reconstructing elastic fibers and rejuvenating endogenously aged skin","authors":"Yi Yang ,&nbsp;Wenjie Huang ,&nbsp;Jingjing Shi ,&nbsp;Bei Tian ,&nbsp;Caihong Fu ,&nbsp;Jianxi Xiao","doi":"10.1016/j.mtbio.2025.102270","DOIUrl":"10.1016/j.mtbio.2025.102270","url":null,"abstract":"<div><div>Endogenous skin aging results in oxidative stress and degradation of elastic fibers, leading to reduced skin elasticity and dermal laxity. Current treatments rarely target elastic fiber regeneration. Here, we introduce a bioactive gel containing recombinant humanized elastin (RHE) that effectively restores skin elasticity and promotes extracellular matrix remodeling. RHE gel demonstrated superior efficacy in reversing UV-induced damage and oxidative stress in zebrafish models, and significantly improved skin function in aged mice, outperforming commercial vitamin E and recombinant humanized collagen (RHC) gels. Molecular analyses revealed enhanced expression of key elastic fiber and collagen components alongside reduced matrix-degrading enzymes. This study highlights RHE gel's potential as a novel therapeutic for regenerative dermatology and skin aging.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102270"},"PeriodicalIF":10.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-incorporated hydrogels loaded with curcumin microspheres for the repair of bone defects","authors":"Wencan Lu ,&nbsp;Weida Zhuang ,&nbsp;Wenhua Li ,&nbsp;Hongxun Sang","doi":"10.1016/j.mtbio.2025.102264","DOIUrl":"10.1016/j.mtbio.2025.102264","url":null,"abstract":"<div><div>Addressing bone defects continues to be an exceedingly difficult challenge owing to local immune dysregulation, vascular injury, and disorders of osteogenesis. No entirely satisfactory resolution has been achieved. In this work, through loading curcumin/poly (lactic-co-glycolic acid) microspheres constructed via a double emulsion technique, a copper-incorporated carboxymethyl chitosan/sodium alginate hydrogel (CA-Cur@Cu hydrogel) was developed. The CA-Cur@Cu hydrogels can sustain the release of curcumin for a long time. This sustained release demonstrates effective antioxidant and anti-inflammatory properties, inducing M2 polarization of macrophages and enhancing the bone immune microenvironment. Furthermore, Cu²⁺ released from the CA-Cur@Cu hydrogels induces angiogenesis and osteogenesis, which synergize with anti-inflammatory properties of curcumin to accelerate the repair of bone defects. Additionally, the CA-Cur@Cu hydrogels exhibit remarkable antibacterial activity, effectively inhibiting clinical bacterial infections. Overall, this study successfully constructed a multifunctional hydrogel integrating antioxidant, anti-inflammatory, pro-angiogenic, and osteogenic properties, providing a new perspective and potential solution for the treatment of bone defects.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102264"},"PeriodicalIF":10.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-adsorption of serum albumin on biomaterial surfaces modulates bacteria-surface interactions and alters bacterial physiological responses","authors":"Hung Le ,&nbsp;Marie Droniou ,&nbsp;Lisa Wallart ,&nbsp;Laurent Coquet ,&nbsp;Pascal Thebault ,&nbsp;Clément Guillou ,&nbsp;Pascal Cosette","doi":"10.1016/j.mtbio.2025.102254","DOIUrl":"10.1016/j.mtbio.2025.102254","url":null,"abstract":"<div><div>When a biomedical device is implanted into the body, its surface initially encounters biological fluids, resulting in the natural adsorption of various host proteins. This protein-adsorbed layer alters the inherent properties of the biomaterial surface and plays a crucial role in interactions between the implant and bacteria. Here, we investigated the influence of an adsorbed layer of albumin, the most abundant blood protein, on the adhesion and biofilm formation of three different bacterial strains: <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, and <em>Pseudomonas aeruginosa</em>. We found that the effect of a serum albumin layer on bacterial adhesion was strain-dependent. Albumin pre-adsorption reduced the initial attachment of <em>S. aureus</em> and <em>S. epidermidis</em> to PDMS surfaces but had no impact on <em>P. aeruginosa</em>. However, with prolonged incubation, albumin-coated surfaces significantly promoted <em>P. aeruginosa</em> attachment and biofilm formation. Additionally, this biofilm alteration was associated with reduced macrophage-mediated bacterial clearance. Proteomic analysis further revealed significant physiological changes in <em>P. aeruginosa</em> upon exposure to albumin-coated surfaces compared to uncoated controls. These alterations were particularly associated with molecular pathways involved in surface colonization, including <em>quorum sensing</em>, motility, adhesion, and biofilm formation. These findings suggest that serum albumin adsorption not only affects the initial adhesion of bacteria but also modifies their adaptive responses upon contact with the biomaterial surface. This study provides a deeper understanding of the complex bacteria-surface interactions, contributing to the development of future strategies for preventing implant-associated infections.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102254"},"PeriodicalIF":10.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage-related immune responses to polyetherketoneketone bone implants: Single-cell transcriptome analysis","authors":"Jiannan Zhou ,&nbsp;Huibin Liang ,&nbsp;Jiahao Chen ,&nbsp;An Li ,&nbsp;Jingtao Dai ,&nbsp;Ping Li","doi":"10.1016/j.mtbio.2025.102257","DOIUrl":"10.1016/j.mtbio.2025.102257","url":null,"abstract":"<div><div>Polyetherketoneketone (PEKK) has emerged as a potential alternative to titanium (Ti) for bone implants. Nevertheless, its osseointegration performance is inferior to that of Ti, primarily due to the limited understanding of its early immune reactions. To address this limitation, this study utilized single-cell RNA sequencing to investigate the distinct early macrophage responses triggered by Ti-based and PEKK-based implants. This approach enabled the characterization of macrophage-polarization dynamics and intercellular interactions within the bone-marrow microenvironment post-implantation. The findings revealed a material-dependent dichotomy in macrophage phenotype: Ti implants preferentially recruited <em>Cd99</em>⁺ macrophages, establishing an anti-inflammatory microenvironment that promotes osseointegration. Conversely, PEKK implants recruited <em>Icam1</em>⁺ macrophages, leading to persistent inflammation and hematopoietic stem cells (HSCs) stress. Additionally, Ti surfaces facilitated CD99-dependent crosstalk between macrophages and T cells, enhancing Th2 responses, which are indicative of an anti-inflammatory effect. In contrast, PEKK-associated macrophages triggered ICAM1-driven necroptosis in HSCs, disrupting hematopoietic homeostasis. These results indicate the early macrophage-related responses as key determinants of the clinical-outcome differences between Ti and PEKK implants.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102257"},"PeriodicalIF":10.2,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}