Materials Today Bio最新文献

{"title":"A 3R (remove-remodel-repair)-integrated self-assembled Chlorella-gelatin-PEG hydrogel for diabetic wound healing","authors":"Yulin Li ,&nbsp;Ruiying He ,&nbsp;Yu Huang ,&nbsp;Tinglin Zhang ,&nbsp;Lan Xiao ,&nbsp;Yin Xiao ,&nbsp;Haifeng Liu ,&nbsp;He Bai ,&nbsp;Shiyong Wu ,&nbsp;Minghao Xue ,&nbsp;Huifen Qiang ,&nbsp;Yan Wu ,&nbsp;Meng Li ,&nbsp;Chuan Yin ,&nbsp;Jie Gao","doi":"10.1016/j.mtbio.2025.101935","DOIUrl":"10.1016/j.mtbio.2025.101935","url":null,"abstract":"<div><div>Diabetic wound healing remains a critical clinical challenge due to persistent bacterial infections, oxidative stress, and chronic inflammation. Inspired by the principles of traditional Chinese medicine, we developed a multifunctional hydrogel (CPGel) via solvent-driven self-assembly of bioactive Chlorella extracts, gelatin, and polyethylene glycol (PEG). The CPGel synergizes the 3R strategy: (1) Remove—complete eradication of <em>E. coli</em> and <em>S. aureus</em> via intrinsic antibacterial activity; (2) Remodel—65 % ROS scavenging and immunomodulation by polarizing macrophages from proinflammatory M1 to anti-inflammatory M2 phenotypes to resolve chronic inflammation; and (3) Repair—accelerated tissue regeneration via 9-fold angiogenesis (α-SMA) and 2-fold collagen I deposition. Mechanically robust (compressive strength: 4.16 MPa; tensile strength: 627 kPa) and antifreezing (−100 °C), CPGel demonstrated exceptional transparency and adhesion, ensuring seamless integration with wound sites. In diabetic mice, CPGel achieved 100 % wound closure within 21 days, surpassing controls (73 %), while promoting neovascularization and hair follicle regeneration. This study bridges traditional herbal medicine with advanced biomaterial engineering, offering a scalable, toxin-free platform that addresses the triad of diabetic wound pathogenesis. By harmonizing the 3R strategy-Remove, Remodel, and Repair-CPGel represents a paradigm shift in chronic wound management, with high potential for clinical translation and sustainable therapeutic design.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101935"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Norcantharidin/Cu2+ dual-depleting GSH nanocatalyst with pH-responsive for CT/CDT synergistic cancer therapy","authors":"Xiaohuan Guo ,&nbsp;Bingbing Cai ,&nbsp;Qi Fang,&nbsp;Yanyan Chen,&nbsp;Yuzhu Zhou,&nbsp;Zhixing Liang,&nbsp;Changchun Wen,&nbsp;Yan-Cheng Liu,&nbsp;Hong Liang","doi":"10.1016/j.mtbio.2025.101959","DOIUrl":"10.1016/j.mtbio.2025.101959","url":null,"abstract":"<div><div>The high level of glutathione (GSH) in tumor cells can consume reactive oxygen species (ROS), seriously affecting the efficacy of chemodynamic therapy (CDT). Although it took a great deal of effort, developing a tumor-specific CDT that efficiently depletes GSH remains a formidable challenge. Herein, we propose a pH-responsive nanocatalyst containing the active molecule norcantharidin (NCTD) and Cu²⁺ for dual GSH depletion, achieving efficient GSH depletion. Due to the weakly acidic tumor microenvironment (TME), the catalyst releases NCTD and Cu²⁺ in a pH-responsive manner for the synergistic therapy of chemotherapy (CT) and CDT. Both components consume GSH and subsequently produce ROS, reducing the antioxidant capacity of cancer cells while increasing oxidative stress. This disrupts cellular redox homeostasis, leading to mitochondrial dysfunction and inducing tumor cell apoptosis. This work not only develops nanomaterials with dual GSH depletion capabilities for high-efficiency CDT but also achieves synergistic CT and CDT tumor therapy with the addition of NCTD, an active ingredient of traditional Chinese medicine.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101959"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-driven secondary structural remodeling in biomimetic nanosystem to enhance tumor chemo-phototherapy","authors":"Weijie Wang ,&nbsp;Chenguang Sun ,&nbsp;Linhao Jing ,&nbsp;Yaning Xia ,&nbsp;Shuijun Zhang ,&nbsp;Yupeng Shi","doi":"10.1016/j.mtbio.2025.101955","DOIUrl":"10.1016/j.mtbio.2025.101955","url":null,"abstract":"<div><div>The integration of chemotherapy and phototherapy for treating advanced liver cancer has gained considerable attention. However, challenges such as short drug retention times significantly impact patient prognosis. We introduce a light-triggered nanosystem that employs molecular aggregation control for PTT and sustained chemotherapy. This nanosystem, known as Reg/IR783@CM nanoparticles (RIMNPs), consists of a core-shell carrier-free nanodrug self-assembled from the chemotherapy drug regorafenib (Reg) and the photothermal agent IR783, coated with a homologous liver cancer cell membrane. The developed core-shell nanocarrier exhibits excellent water dispersibility, high drug load, extended blood circulation, and tumor site enrichment. Upon light exposure, the nanosystem provides outstanding near-infrared imaging and robust photothermal effects. Concurrently, light exposure accelerates the degradation of the outer IR783 layer, resulting in regorafenib exposure and triggering secondary assembly, which significantly enhances drug retention at the tumor site. Our findings indicate that the nanosystem effectively suppresses tumor growth by combining photothermal therapy with the inhibition of tumor cell proliferation and angiogenesis, and by modulating tumor-associated macrophages. Notably, this nanosystem also demonstrates low cytotoxicity and high biocompatibility. This study presents a novel light-driven in-situ assembly strategy, offering a simplified and effective approach for constructing tumor imaging and treatment systems.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101955"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgal-enhanced cerium oxide nanotherapeutics for alleviating inflammatory bowel disease via scavenging reactive oxygen species and modulating gut microbiota in colitis","authors":"Xiaoyue Lei ,&nbsp;Qikun Cheng ,&nbsp;Zhenyu Yang ,&nbsp;Huiqun Hu ,&nbsp;Hang Yang ,&nbsp;Wenting Zhang ,&nbsp;Shuyang Zhang ,&nbsp;Ao Sun ,&nbsp;Zengwen Zhang ,&nbsp;Shuli Deng","doi":"10.1016/j.mtbio.2025.101945","DOIUrl":"10.1016/j.mtbio.2025.101945","url":null,"abstract":"<div><div>Inflammatory bowel disease (IBD) poses significant therapeutic challenges due to its complex pathophysiology, which involves oxidative stress and dysbiosis of the intestinal microbiota. Antioxidant nanozymes offer promising intervention options because of their potent reactive oxygen species (ROS)-scavenging abilities; however, their instability and leakage in the upper gastrointestinal tract remain major challenges. This study introduced a novel oral drug delivery system, SP@CSC, which combines Spirulina platensis (SP), a natural microcarrier, with chitosan-functionalized cerium oxide (CSC) nanogels. The CSC nanogels demonstrated superior antioxidant capabilities compared to CeO₂ nanoparticles. The acid-resistant and intestinal retention properties of SP improved the distribution and prolonged the residence time of CSC nanogels in the gut, thereby facilitating targeted antioxidant actions. SP@CSC effectively protected epithelial cells from oxidative stress-induced damage, restored mitochondrial function, and inhibited apoptosis. Additionally, SP@CSC exhibited immunomodulatory effects by suppressing macrophage infiltration and M1 polarization in IBD-associated microenvironments. In the dextran sulfate sodium–induced mouse colitis models, oral administration of SP@CSC alleviated IBD symptoms through restoring intestinal barrier integrity, modulating the immune-microenvironment, and enhancing the abundance and diversity of gut microbiota. These findings highlighted the potential of SP@CSC as a promising candidate for IBD treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101945"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A microsphere loaded with chondrocyte-targeting exosomes continuously deliver miR-148a for osteoarthritis therapy","authors":"Tao Yang ,&nbsp;Haiwei Ma ,&nbsp;Kechi Li ,&nbsp;Jie Xiang ,&nbsp;Jianxin Qiu ,&nbsp;Yiming Ding ,&nbsp;Chengdi Wang ,&nbsp;Hehuan Lai ,&nbsp;Xingyu Hu ,&nbsp;Shijie Liu ,&nbsp;Chao Lou ,&nbsp;Zhenhua Hong ,&nbsp;Zhenzhong Chen ,&nbsp;Dengwei He","doi":"10.1016/j.mtbio.2025.101944","DOIUrl":"10.1016/j.mtbio.2025.101944","url":null,"abstract":"<div><div>Due to the complex pathogenesis and limited treatment options, the clinical diagnosis and treatment of osteoarthritis (OA) remains extremely challenging. Umbilical cord derived mesenchymal stem cell-derived exosomes have low immunogenicity, high biocompatibility and superior transbiofilm properties, showing great research value in OA treatment. Healthy chondrocytic extracellular matrix anabolism is essential for cartilage homeostasis. The abnormal reduction of miR-148a in chondrocytes impairs extracellular matrix anabolism, accelerating the progression of OA. Therefore, increasing miR-148a in chondrocytes is a potential OA treatment strategy. However, the dense extracellular matrix in articular cartilage hinders the delivery of exosomes and microRNAs, in addition to their short retention time <em>in vivo</em> greatly limits their clinical application. Herein, a biodegradable microsphere loaded with chondrocyte targeting exosomes (CAP-EXOs/miR-148a@GAM) was developed for sustained delivery of miR-148a. Firstly, we synthesized microspheres (GAM) with methacrylated gelatin and streptavidin by microfluidic system, and then loaded miR-148a into exosomes modified with chondro-targeting peptide CAP (CAP-EXOs) and encapsulated it into GAM to obtain CAP-EXOs/miR148a@GAM. The <em>in-vitro</em> results show that this microsphere promotes the anabolism of chondrocytes and exhibits satisfactory performance in maintaining cartilage homeostasis. The <em>in-vivo</em> experiments show that the microspheres can effectively delay the degeneration of osteoarthritic cartilage. Collectively, this study presents a multifunctional paradigm of nucleic acid drug delivery for the treatment of OA.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101944"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-entropy nanozyme-driven \"Chinese acupuncture\" hydrogel microneedles for combined therapy of infected scalds","authors":"Hui Li ,&nbsp;Yuwei Ren ,&nbsp;Ying Zhan ,&nbsp;Xiaoyu Yu ,&nbsp;Xin Zhang ,&nbsp;Hongzhou Chen ,&nbsp;Qi Li ,&nbsp;Jianghua Cheng ,&nbsp;Yingwang Ye","doi":"10.1016/j.mtbio.2025.101953","DOIUrl":"10.1016/j.mtbio.2025.101953","url":null,"abstract":"<div><div>Bacterial infections, especially those caused by antibiotic-resistant <em>Listeria monocytogenes</em> (<em>L. monocytogenes</em>), hinder the healing of scalds in food processing environments. This study developed a high-entropy nanozyme (HEzyme) hydrogel system that exhibited efficient reactive oxygen species (ROS) and photothermal antibacterial effects. The HEzyme was synthesized using a carbon-oxygen-silicon framework-assisted method, incorporating multiple metal species (CoNiCuZnCe). It demonstrated superior catalytic efficiency, generating hydroxyl radicals (•OH) from hydrogen peroxide (H₂O₂). Exposure to 808 nm irradiation caused a rapid temperature increase, enabling photothermal effect. HEzyme was integrated into a chitosan-based hydrogel microneedle system, which generated ROS and heat when exposed to H₂O₂ and irradiation. The microneedles, designed for transdermal use, enhanced wound healing and minimized inflammation. In vitro and in vivo tests confirmed that the microneedles effectively eradicated <em>L. monocytogenes</em> and accelerated tissue healing. This approach offers a promising solution for treating antibiotic-resistant infections and promoting healing in scald wounds.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101953"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alchemizing earth's legacy: Bismuth-engineered humic nanoparticles for IBD theranostics through mitochondrial anti-inflammation and sustained intestinal delivery","authors":"Ganglin Wang ,&nbsp;Ziwei Wang ,&nbsp;Lin Liu ,&nbsp;Yejing Zhu ,&nbsp;Jiali Zhong ,&nbsp;Jiayi Zhang ,&nbsp;Lingling Wang ,&nbsp;Chenguo Zheng ,&nbsp;Wei Li","doi":"10.1016/j.mtbio.2025.101948","DOIUrl":"10.1016/j.mtbio.2025.101948","url":null,"abstract":"<div><div>Humic acid (HA), a complex organic substance naturally present in soil, peat, and coal, is traditionally referred to as the extract of <em>\"Wujinshi\"</em> (乌金石) in Chinese medicine. It is renowned for its potent anti-inflammatory, analgesic, and blood circulation-promoting properties. However, its mechanisms and delivery methods require further exploration. We developed BiHANs, a novel nanoparticle combining bismuth and HA, which exhibits excellent biocompatibility and anti-inflammatory effects <em>in vitro</em>. Orally administered BiHANs accumulate at inflammatory sites in the colon of DSS-induced IBD mice, as confirmed by CT imaging, and prolong intestinal retention. They significantly alleviate acute and chronic intestinal damage by suppressing inflammatory responses, modulating gut microbiota, and targeting macrophage mitochondria to inhibit NF-κB-driven cytokine production. BiHANs also maintain mitochondrial homeostasis and exert antioxidative effects. Furthermore, their performance in CT imaging highlights potential as a theranostic agent. This study demonstrates BiHANs as a promising platform for targeted IBD therapy and diagnosis, combining anti-inflammatory efficacy with imaging capabilities.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101948"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix-degradable polymer nanostimulants elicit potent immune responses in orthotopic pancreatic cancer via sono-activatable dual-drug synergism","authors":"Meng Li ,&nbsp;Danling Cheng ,&nbsp;Yue Wang ,&nbsp;Chongwen Xuan ,&nbsp;Viktar Abashkin ,&nbsp;Jindong Xia ,&nbsp;Ling Ding ,&nbsp;Jingchao Li","doi":"10.1016/j.mtbio.2025.101954","DOIUrl":"10.1016/j.mtbio.2025.101954","url":null,"abstract":"<div><div>Pancreatic cancer is a highly aggressive malignancy with a poor prognosis due to its complex tumor microenvironment (TME), which includes a dense extracellular matrix (ECM) and immunosuppressive pathways. Nanomedicine capable of achieving profound tumor penetration and modulating the tumor immune microenvironment is urgently needed to enhance the efficacy of cancer therapy. Herein, we introduce ECM-degradable semiconducting polymer nanostimulants (SPNs) as a novel nanostimulant for deep tumor penetration and multifaceted remodeling of the tumor microenvironment. The SPNs were constructed by loading two immune drugs: toll-like receptor 7/8 agonist (R848) and indoleamine 2,3-dioxygenase inhibitor (NLG919), onto singlet oxygen (¹O₂)-responsive SPNs, and modifying their surface with hyaluronidase (HAase). Upon accumulation at orthotopic pancreatic tumor sites, HAase-mediated degradation of the ECM significantly enhances the penetration of nanomedicine into the tumor and facilitates the infiltration of immune cells. Upon sono-activation, the SPNs produce ¹O₂, which is not only used for sonodynamic therapy of deep-seated pancreatic tumors and, but also induces immunogenic cell death (ICD) in tumor cells. Simultaneously, the generated ¹O₂ can be cleaved by ¹O₂-responsive fragments, disrupting the nanoparticle structure and enabling controlled and precise release of the two immune drugs at the tumor site, thus minimizing off-target effects. Through such a multifaceted remodeling mechanism, SPN-based treatment triggers a potent antitumor immunological response. Consequently, the growth of orthotopic pancreatic tumors in mouse models is nearly inhibited, and tumor metastases are effectively suppressed. This study presents an ECM-degradable semiconducting polymer nanostimulant for multifaceted remodeling of the tumor microenvironment, enabling effective and precise immunotherapy of deep-seated orthotopic tumors.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101954"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR light-propelled Janus Polydopamine@Mesoporous silica gene delivery nanomotor for the enhanced gene treatment of critical limb ischemia","authors":"Liang Gui ,&nbsp;Suyu Wang ,&nbsp;Suyu Miao ,&nbsp;Minghang Qiao ,&nbsp;Yuanyong Jiao ,&nbsp;Haibin Chen ,&nbsp;WanYang Li ,&nbsp;Junjie Zou ,&nbsp;Xin Du","doi":"10.1016/j.mtbio.2025.101950","DOIUrl":"10.1016/j.mtbio.2025.101950","url":null,"abstract":"<div><div>Among multiple non-invasive therapeutic approaches for critical limb ischemia (CLI), gene therapy has been widely researched because of its capacity to ensure continuous expression and growth factors release. Hypoxia-inducible factor-1α (HIF-1α), a gene that can promote stable angiogenesis and tissue restoration, is a potential candidate for facilitating cellular adaptation to hypoxia during vascular injury recovery. Herein, we designed a near-infrared (NIR) light-propelled pEX-1/HIF-1α plasmid DNA (pDNA) loaded Janus polydopamine@mesoporous silica (PDA@MS) nanomotors (PDA@MS-NH₂@HIF-1α) with asymmetric yolk@shell structure and rough particle surface containing large pores. The PDA@MS nanomotors have a rough and porous particle surface that is modified with positively charged aminopropyl. This modification enables efficient electrostatic absorption of negatively charged pDNA, resulting in a high loading capacity. The distribution of yolk in the asymmetric PDA enables the creation of a localized thermal gradient field around the PDA@MS when exposed to irradiation with a low-energy-intensity NIR laser. This allows the PDA@MS to move via a self-thermophoretic mechanism. The NIR light propulsion promotes the efficient delivery of HIF-1α-pDNA by PDA@MS-NH₂@HIF-1α nanomotor. Due to the good antioxidant activity of PDA, PDA@MS-NH₂@HIF-1α nanomotors exhibit exceptional biocompatibility and significantly enhance the ischemic microenvironment. In vitro and in vivo outcomes verify that PDA@MS-NH₂@HIF-1α nanomotors have enhanced pro-angiogenic capacity and improved gene transfection efficiency. Furthermore, the therapeutic efficacy of PDA@MS-NH₂@HIF-1α is assessed using a murine hindlimb ischemia model. The results show that the intramuscular injection of PDA@MS-NH₂@HIF-1α combined with NIR light irradiation leads to a significant improvement in blood flow restoration and muscle repair. When considering these findings collectively, this kind of gene-delivery nanomotor has the potential to be a promising paradigm for future CLI treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101950"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow-induced mechano-modulation of intestinal permeability on chip","authors":"Raffaele Mennella ,&nbsp;Sara Sibilio ,&nbsp;Francesco Urciuolo ,&nbsp;Giorgia Imparato ,&nbsp;Paolo A. Netti","doi":"10.1016/j.mtbio.2025.101951","DOIUrl":"10.1016/j.mtbio.2025.101951","url":null,"abstract":"<div><div>The comprehension of the working principles behind the intestinal transepithelial transport is critical in nutrient and drug development research. Within this framework, microfluidic microphysiological platforms are on the verge of overshadowing traditional <em>in vitro</em> systems due to their accuracy in replicating key physiological features of the native tissue. Nevertheless, the effects of fluid mechanical stimuli on the selective permeation characteristics of the gut barrier are still unexplored. This is an indispensable feature for designing more biorelevant organ-on-chip models. Here, an intestine-on-chip platform is conceived to mechanically stimulate cells with three different fluid shear stresses and investigate the relative flow-induced changes of molecule transport alongside the resulting epithelial architecture and barrier functionality. Our results reveal that epithelia grown at lower shears exhibit a ∼1.5 higher and faster paracellular permeability while showing a ∼3 times lower and delayed transcellular uptake compared to layers exposed to higher shear stress. This is corroborated by impedance spectroscopy measurements that display altered tight junctional and bilayer resistance, as well as an increased capacitance of the epithelium in response to variations in mechanical stress within the culture. Taken together, these findings advocate that fluid shear stress can serve as mechano-modulator not only for intestinal transport but also for other epithelial cell lines under physiological circumstances.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101951"},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}