Materials Today Bio最新文献

{"title":"Stealth missiles with precision guidance: A novel multifunctional nano-drug delivery system based on biomimetic cell membrane coating technology","authors":"Yuyan Zhou ,&nbsp;Xinyue Wang ,&nbsp;Xiaorong Tian ,&nbsp;Deyu Zhang ,&nbsp;Hanxiao Cui ,&nbsp;Wei Du ,&nbsp;Zhenghui Yang ,&nbsp;Jiayu Li ,&nbsp;Wanshun Li ,&nbsp;Jiaheng Xu ,&nbsp;Ying Duanmu ,&nbsp;Ting Yu ,&nbsp;Fengping Cai ,&nbsp;Wenhao Li ,&nbsp;Zhendong Jin ,&nbsp;Wencheng Wu ,&nbsp;Haojie Huang","doi":"10.1016/j.mtbio.2025.101922","DOIUrl":"10.1016/j.mtbio.2025.101922","url":null,"abstract":"<div><div>Nanodrug delivery systems (NDDSs) have demonstrated broad application prospects in disease treatment, prevention, and diagnosis due to their nanoscale size advantages and high drug-loading capacity. However, their clinical translation still faces multiple challenges, including rapid clearance by the reticuloendothelial system (RES), nonspecific targeting, and insufficient efficiency in crossing biological barriers. Cell membrane-coated biomimetic delivery systems (CMC-BDS), which integrates natural cell membranes onto nanoparticle (NPs) surfaces, provides nanodrugs with a versatile \"biomimetic cloak,\" representing a highly promising surface engineering strategy. This approach enables nanocarriers to inherit the intrinsic biological properties of different cell sources, endowing them with immune evasion, prolonged circulation, dynamic targeting, biocompatibility, and biodegradability, while supporting the integration of diverse biomedical functions. Furthermore, surface functionalization modifications can enhance their programmability, multifunctionality, and biointerface adaptability, thereby optimizing targeted delivery efficiency and extending in vivo circulation time. This review first outlines the development and key preparation steps of cell membrane coating technology. It then discusses the selection strategies for various cell membrane types—including leukocyte, erythrocyte, platelet, dendritic cell, tumor cell, and bacterial membranes—while comparing their respective advantages and limitations. Finally, the review highlights recent advances in applying cell membrane-coated nanoparticles (CMC-NPs) for treating tumors, ischemic stroke, and inflammatory diseases.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101922"},"PeriodicalIF":8.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR-responsive tissue-adaptive hydrogel for accelerating healing of seawater-immersed wounds.","authors":"Rui Ma, Li Xu, Ze Li, Sicheng Li, Ye Liu, Guiwen Qu, Kang Chen, Canwen Chen, Luqiao Huang, Yitian Teng, Xinxin Huang, Shuanghong Yang, Qingchuan Li, Jinjian Huang, Jianan Ren, Xiuwen Wu","doi":"10.1016/j.mtbio.2025.101915","DOIUrl":"10.1016/j.mtbio.2025.101915","url":null,"abstract":"<p><p>Seawater-immersed wounds pose a significant health risk owing to the high-salt and hypertonic environment of seawater, and the presence of various bacterial species, notably <i>Vibrio vulnificus (V. vulnificus)</i>. Although a number of dynamically adhesive hydrogels have developed recently, these gels are often composed of dynamic networks, which lead to insufficient mechanical strength and short-term protection for wound surfaces. In this study, a GPS hydrogel with a covalent network based on gelatin-methacryloyl (GelMA), poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) (SBMA) has been developed. The GPS hydrogel demonstrated significantly enhanced mechanical properties compared with GelMA hydrogel, exhibiting 18-fold (501 % strain) and 299-fold (613 kJ/m³) improvements in tensile strain and toughness, respectively, along with a Young's modulus of 50.1 kPa and rapid 160 s gelation capability. Furthermore, the GPS hydrogel achieved exceptional photothermal conversion under 808 nm NIR irradiation, attaining 57 °C within 100 s to enable near-complete bacterial eradication (100 %). This study presented the first transcriptomic profiling of <i>V. vulnificus</i> following photothermal treatment (PTT). Our analysis revealed significant membrane disruption, attenuation of virulence determinants, and global metabolic reprogramming in PTT-treated bacterial cells. <i>In vitro</i> assessments demonstrated the hydrogel's biocompatibility, while <i>in vivo</i> evaluations revealed that GPS hydrogel significantly enhanced seawater immersed wound healing rates in rat models. Altogether, this study offered a promising solution for the long-term management of seawater-immersed wounds with a covalently-crosslinked photothermal antibacterial hydrogel dressing.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"101915"},"PeriodicalIF":8.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microneedle-based sustained release delivery of TNF-α/IL-6R dual-specific fenobody alleviates inflammation and promotes bone regeneration in rheumatoid arthritis rat model","authors":"Xiqian Zhang ,&nbsp;Jian Chen ,&nbsp;Na Huang ,&nbsp;Qi Chen ,&nbsp;Muhammad Asad Farooq ,&nbsp;Ping Ouyang ,&nbsp;Kaisong Huang ,&nbsp;Kangsheng Liao ,&nbsp;Wanjun He ,&nbsp;Kai Cui ,&nbsp;Dan Jiang ,&nbsp;Guangxian Xu","doi":"10.1016/j.mtbio.2025.101905","DOIUrl":"10.1016/j.mtbio.2025.101905","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease characterized by inflammatory imbalance. The cytokine-targeted therapy is a non-traditional form of RA treatment that is mighty effective but poses the challenge of pharmaceutical storage conditions, professional injection, frequent injection administrations and poor patient adherence, and due to individual differences in sensitivity to TNF-α and IL-6R, monotherapy with a single antibody is effective in fewer than 30 % of cases. Hence, to address these issues, we have developed a macromolecule polymer GelMA microneedle sustained-release platform for transdermal delivery of a dual-specific fenobody targeting TNF-α and IL-6R in an arthritic rat model. The results demonstrated that the IL-6R-TNF-α-fenobody exhibited enhanced specificity and affinity and an extended half-life. IL-6R-TNF-α-fenobody effectively neutralizes rhTNF-α-induced cytotoxicity in L929 cells, while inhibiting the phosphorylation of IκBα and p65 in the classical NF-κB signaling pathway, and significantly blocks JAK-dependent phosphorylation of STAT3, thereby exerting its anti-inflammatory effects via multiple signaling pathways. The GelMA hydrogel microneedle sustained-release platform facilitates controlled cargo delivery to alleviate the inflammatory environment and reduce over-activated synoviocyte activity, thus promoting bone and joint regeneration. In collagen-induced arthritis (CIA) rats, cross-linked gelatin microneedles demonstrated high mechanical strength, facilitating the effective delivery of fenobodies to the dermis for optimal microcirculation, reduced levels of inflammatory factors, this strategy significantly inhibited the progression of RA. Overall, the IL-6R-TNF-α-Fenobody therapy promoted bone and joint regeneration by synergistic inflammation-resolving, osteogenesis. Excitingly, the microneedle group showed comparable treatment outcomes to the injection group, providing a portable, painless, sustained-release alternative that could enhance patient compliance by reducing the need for frequent professional injections.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101905"},"PeriodicalIF":8.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative progress and clinical utilization of platinum-derived nanotherapeutics for chronic wound healing.","authors":"Jie Yang, Qingyu Han, Liping Li, Ruiping Zhang","doi":"10.1016/j.mtbio.2025.101929","DOIUrl":"10.1016/j.mtbio.2025.101929","url":null,"abstract":"<p><p>Chronic wounds are a major public health concern worldwide because their complex pathophysiological processes and multifactorial etiology substantially complicate therapeutic interventions, highlighting the urgency of developing novel intervention approaches. Platinum-derived (Pt-derived) nanomaterials, owing to their distinctive biophysical characteristics and catalytic functionalities, have demonstrated promising therapeutic capabilities in chronic wound management. Nevertheless, critical gaps remain in understanding their clinical translation and effectiveness. In this context, the present review systematically investigated the structure-bioactivity relationships of Pt-derived nanocomposites and hybrid systems while evaluating their biomedical multivalency and combinatory approaches in therapeutic applications. In addition, the limitations currently impeding the practical implementation of Pt-derived nanotherapeutics and prospects for future advancements in material optimisation and biological integration strategies are addressed.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"101929"},"PeriodicalIF":8.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermosensitive hydrogel composite with si-Cx43 nanoparticles and anti-VEGF agent for synergistic treatment of diabetic retinopathy","authors":"Lihui Wen ,&nbsp;Mengxin Gan ,&nbsp;Siying Xiong ,&nbsp;Li Dai ,&nbsp;Wen Chen ,&nbsp;Wen Shi","doi":"10.1016/j.mtbio.2025.101917","DOIUrl":"10.1016/j.mtbio.2025.101917","url":null,"abstract":"<div><div>Diabetic retinopathy (DR) is characterized by pathological angiogenesis, inflammation, and retinal neurodegeneration, leading to vision loss. Current therapies, such as anti-VEGF agents, face challenges of low bioavailability and frequent invasive injections. Connexin43 (Cx43), a gap junction protein, plays a key role in DR progression through its modulation of inflammation and vascular dysfunction. A thermosensitive hydrogel composite was developed to encapsulate siRNA targeting Cx43 (si-Cx43) nanoparticles (NPs) and anti-VEGF (Avastin). The hydrogel was characterized for gelation, injectability, and degradation. <em>In vitro</em> studies evaluated the cytotoxicity, anti-angiogenic effects, and permeability regulation in hyperglycemic retinal cells under hyperglycemic conditions. <em>In vivo</em> therapeutic efficacy was assessed in a diabetic retinopathy rat model. si-Cx43-NPs demonstrated high siRNA encapsulation efficiency and stability, effectively silencing Cx43 expression in retinal endothelial cells. The hydrogel exhibited excellent injectability, temperature-sensitive gelation, and controlled degradation. In vitro, si-Cx43-NPs@Avastin-hydrogel significantly suppressed VEGF expression, reduced angiogenesis, and restored cell permeability under hyperglycemic conditions. <em>In vivo</em>, the hydrogel composite reduced neovascularization, inflammation, and apoptosis, restoring retinal structure and function more effectively than either single-agent treatment alone. Biocompatibility studies confirmed minimal toxicity and favorable degradation. The si-Cx43-NPs@Avastin-hydrogel provides a synergistic and minimally invasive therapeutic strategy for DR by targeting angiogenesis, inflammation, and neuroprotection with sustained drug delivery.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101917"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A glutathione-responsive ferroptotic inducer with elevated labile iron pool and self-supplied peroxide for chemodynamic therapy.","authors":"Penghui Wei, Xuegang Niu, Dengliang Wang, Chengzhong Du, Mingtao Zhu, Hongjia Zheng, Yongrui Hu, Yu Tian, Wei Huang, Chengyu Ding, Yuanxiang Lin, Yang Zhu, Dezhi Kang","doi":"10.1016/j.mtbio.2025.101913","DOIUrl":"10.1016/j.mtbio.2025.101913","url":null,"abstract":"<p><p>Chemodynamic therapy (CDT) is a novel approach in the treatment of tumors in which ferrous iron (Fe²⁺) is the primary catalyst of the Fenton reaction. However, Fe²⁺ is typically stored in an oxidized mineral form as ferric iron (Fe³⁺) in ferritin, significantly limiting the efficacy of CDT. This work describes the preparation of redox-responsive nanoparticles (MO@DSSP NPs) embedded with OSMI-1 and methyl linoleate hydroperoxide (MLH) to synergistically enhance CDT efficacy, optimize peroxide supply and deplete glutathione (GSH). The redox-responsive MO@DSSP NPs undergo disintegration after being internalized by tumor cells due to the reductive tumor microenvironment, consuming GSH while releasing OSMI-1 and MLH. This process increases the intracellular labile iron pool (LIP) and oxidative stress at the tumor site by inhibiting O-GlcNAcylation of ferritin heavy chain (FTH). Furthermore, obstructing O-GlcNAc modification triggers mitochondrial fragmentation alongside autophagy, thus contributing an extra source of reactive iron. The increased LIP significantly promotes the generation of hydroxyl radical (·OH) that causes lipid peroxidation, consequent damage of the cell membrane and ferroptosis. Therefore, this study describes an attractive CDT nanoagent that effectively inhibits the O-GlcNAcylation of FTH to mobilize endogenous Fenton-type metals, as well as offers a basis to the exploration of LIP-activatable MLH with high CDT efficacy, demonstrating significant potential for clinical applications.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"101913"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging femtosecond laser machining for the fabrication of tubular-based Organ-on-Chip systems: modeling cancer metastasis from invasion to intravasation","authors":"Mohammad Jouybar ,&nbsp;Oscar Stassen ,&nbsp;Hamed Moradi ,&nbsp;Pan Zuo ,&nbsp;Jaap M.J. den Toonder","doi":"10.1016/j.mtbio.2025.101926","DOIUrl":"10.1016/j.mtbio.2025.101926","url":null,"abstract":"<div><div>Organ-on-Chip (OoC) models often include microchannel-based vessels and ducts with rectangular cross-sections, and therefore these lack the geometry and morphology found in tubular structures in vivo. Channels with round cross-sections can better mimic the physiology and cellular behavior of tubular structures, such as (micro)vessels and breast ducts, by providing a more <em>in vivo</em>-like geometry. Here, we utilize femtosecond laser machining to integrate tubular channels in an Organ-on-Chip device; our \"Lumina-Chip\" contains two tubular channels, both connected to a central channel along their entire length. This versatile fabrication technique, combined with replica molding, enables us to obtain a medium-throughput version of the device, including nine Lumina-Chips. In this study, we showcase the Lumina-Chip's capability by modeling breast cancer invasion, migration, and intravasation, all within a single device as a representative application. We use the device to observe the progression of breast cancer cells from a breast duct (formed in the first lumen, lined with normal epithelial cells), through an extracellular matrix (comprised of collagen I in the central channel), and ultimately into a vessel (formed in the second lumen, lined with endothelial cells). A permeability analysis confirms that the vessel wall maintains strong barrier functionality in the absence of cancer cells. Two types of breast cancer tumoroids (invasive and non-invasive) introduced into the breast duct exhibit distinctly different invasive behaviors.</div><div>While we present breast cancer metastasis as a showcase application, the Lumina-Chip also holds potential for other biological applications where epithelial ducts and vessels with tubular structures are critical components.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101926"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strontium-loaded multifunctional gelatin methacryloyl hydrogels for type-H vascularized bone regeneration under osteoporotic conditions.","authors":"Yuwei He, Fanchun Zeng, Hongyu Quan, Lanyi Liu, Jingjin Dai, Hong Jiang, Shiwu Dong","doi":"10.1016/j.mtbio.2025.101909","DOIUrl":"10.1016/j.mtbio.2025.101909","url":null,"abstract":"<p><p>Osteoporosis (OP) is a clinically prevalent bone disease, under which excessive activation of osteoclasts (OCs) significantly delays bone regeneration. In this context, bone regeneration necessitates not merely the facilitation of osteogenesis and the suppression of resorption, but also more stringent requirements pertaining to vascularization, particularly type-H blood vessels. Recently, strontium (Sr) has emerged as a \"dual-action bone agent\", enhancing bone formation while suppressing bone resorption, yet still receives inadequate attention. Given that PDGF-BB secreted by osteoclast progenitors (pOCs) could induce type-H-related vascularization during coupling with osteogenesis, the regulatory effect of Sr²⁺ on osteoclastogenesis need to be further studied and utilized in vascularized bone regeneration. Here, we synthesized Sr-substituted layered double hydroxide (Sr-LDH) by partially substituting divalent metal ions in LDH with Sr²⁺, and encapsulated the surface-modified Sr-LDH into QK peptide-incorporated gelatin methacryloyl (GelMA) to form a composite hydrogel GelMA-QK/Sr-LDH@PDA (GLQ). This multifunctional hydrogel integrated the osteogenic and antiresorptive properties of Sr-LDH, exhibited significant bone regeneration efficacy under osteoporotic conditions. Furthermore, our study found that GLQ could stimulate bone marrow derived macrophages (BMMs) proliferation and preserve pOCs while suppress OC maturation, which further facilitated the expression of PDGF-BB and promoted type-H vessels development in the bone defect area. Taken together, the multifunctional composite hydrogel holds significant clinical implications for future treatment of osteoporotic bone defects.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"101909"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phase-transition engineered semi-metallic Cu₃PdN for photothermal-enhanced cuproptosis-induced cancer therapy.","authors":"Yao Gao, Guangru Li, Shuoxun Chen, Weijie Yu, Shuanglong Yi, Yu Chen, Luodan Yu","doi":"10.1016/j.mtbio.2025.101927","DOIUrl":"10.1016/j.mtbio.2025.101927","url":null,"abstract":"<p><p>In-situ activation of cuproptosis shows considerable promise in cancer therapy. However, its efficacy is often hindered by the accumulation of copper ions and limitations of the activation strategy. Herein, a novel copper-palladium nitride (Cu₃PdN)-modified injectable hydrogel with enhanced photothermal conversion efficiency was designed to improve copper accumulation at tumor sites and achieve photothermia-enhanced cuproptosis. A phase transition from Cu₃N to Pd-doped Cu₃PdN was achieved, transforming the material from a semiconductor to a semi-metal with a reduced band gap. This modification endowed Cu₃PdN with full-spectrum absorption and enhanced photothermal conversion efficiency. Coupled with the abundant copper content, Cu₃PdN nanoparticles hold great potential for photothermal-enhanced, in-situ cuproptosis-based cancer therapy. The Cu₃PdN system demonstrates stimulus-responsive Cu(I) ions release and Fenton-like activity, promoting the generation of reactive oxygen species, mitochondrial dysfunction, and the oligomerization of dihydrolipoamide S-acetyltransferase, which together trigger cuproptosis. Overall, this study provides a promising approach for utilizing metal nitrides to induce photothermal-enhanced cuproptosis in tumor therapy.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"101927"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface bio-engineering of melt electrowritten tubular scaffolds via plasma immersion ion implantation (PIII)","authors":"Anyu Zhang ,&nbsp;Anne Metje van Genderen ,&nbsp;Bingyan Liu ,&nbsp;Junyi Qian ,&nbsp;Jirawat Iamsamang ,&nbsp;Ziyu Wang ,&nbsp;Miguel Castilho ,&nbsp;Behnam Akhavan","doi":"10.1016/j.mtbio.2025.101923","DOIUrl":"10.1016/j.mtbio.2025.101923","url":null,"abstract":"<div><div>Melt electrowriting (MEW) enables the fabrication of highly controlled, open-pore tubular constructs for replicating the complex architectures of vascular, renal, and other tissues. However, a key challenge is to functionalize their surfaces so that they not only support but also instruct key biological interactions, particularly in promoting vascularization. Here, we propose plasma immersion ion implantation (PIII) as a biofunctionalization strategy for open-pore tubular constructs fabricated by MEW. Surface chemistry analysis confirmed homogeneous treatment across PIII-treated MEW 3D structures, while uniaxial tensile tests demonstrated no significant changes in mechanical properties following the treatment. Electron paramagnetic resonance (EPR) data provided evidence of the formation of a stable, radical-rich surface, which was further validated by fluorescence imaging with a model molecule, confirming the radicals’ role in enabling uniform covalent biomolecule attachment. The PIII-treated MEW constructs were covalently functionalized with vascular endothelial growth factor (VEGF), thereby modulating the behavior of seeded cells. Endothelialization studies using conditionally immortalized glomerular endothelial cells (ciGEnC) demonstrated that VEGF-immobilized MEW tubes effectively support monolayer formation, achieving outcomes comparable to those observed with VEGF supplementation in culture media. Remarkably, the immobilized VEGF sustained endothelialization with a similar effectiveness to traditional VEGF suspension methods over prolonged culture conditions (21 days), but without the need for continuous VEGF supplementation. These findings establish a novel biofunctionalization strategy for vascularized tissue engineering scaffolds and pave the way for plasma-modified MEW tubes as platforms for preclinical models and regenerative medicine applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101923"},"PeriodicalIF":8.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}