Materials Today Bio最新文献_第10页

Polymeric PD1/PDL1 bispecific antibody enhances immune checkpoint blockade therapy 聚合 PD1/PDL1 双特异性抗体能增强免疫检查点阻断疗法的效果

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-14 DOI: 10.1016/j.mtbio.2024.101239

Fuxin Xue , Xitong Ren , Chaoying Kong , Jianfeng Wang , Linlin Liu , Junli Hu , Na Shen , Zhaohui Tang

{"title":"Polymeric PD1/PDL1 bispecific antibody enhances immune checkpoint blockade therapy","authors":"Fuxin Xue , Xitong Ren , Chaoying Kong , Jianfeng Wang , Linlin Liu , Junli Hu , Na Shen , Zhaohui Tang","doi":"10.1016/j.mtbio.2024.101239","DOIUrl":"10.1016/j.mtbio.2024.101239","url":null,"abstract":"<div><p>Immune checkpoint blockade (ICB) therapy, particularly PD1/PDL1 inhibition, has demonstrated success in bolstering durable responses in patients. However, the response rate remains below 30 %. In this study, we developed a polymeric bispecific antibody (BsAb) targeting PD1/PDL1 to enhance ICB therapy. Specifically, poly(<sub>L</sub>-glutamic acid) (PGLU) was conjugated with a double cyclic Fc binding peptide, Fc-III-4C, through condensation reactions between the -COOH group of PGLU and the -NH<sub>2</sub> group of Fc-III-4C. This conjugate was then mixed with αPD1 and αPDL1 monoclonal antibodies (mAbs) in an aqueous solution. Mechanistically, the PD1/PDL1 BsAb (BsAb<sub>αPD1+αPDL1</sub>) acts as a bridge between tumor cells and CD8<sup>+</sup> T cells, continuously activating CD8<sup>+</sup> T cells to a greater extent. This leads to significantly suppressed tumor growth and prolonged survival in a mouse model of colon cancer compared to treatment with either a single mAb or a mixture of free mAbs. The tumor suppression rate achieved by the BsAb<sub>αPD1+αPDL1</sub> was 90.1 %, with a corresponding survival rate of 83.3 % after 48 days. Thus, this study underscores the effectiveness of the BsAb<sub>αPD1+αPDL1</sub> as a synchronizing T cell engager and dual ICBs, offering theoretical guidance for clinical ICB therapy.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101239"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003004/pdfft?md5=9b006b2ebddadc48b0fc7609aa138200&pid=1-s2.0-S2590006424003004-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional vitamin D-incorporated PLGA scaffold with BMP/VEGF-overexpressed tonsil-derived MSC via CRISPR/Cas9 for bone tissue regeneration 通过 CRISPR/Cas9 表达扁桃体来源间充质干细胞的多功能维生素 D 嵌入 PLGA 支架用于骨组织再生

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-14 DOI: 10.1016/j.mtbio.2024.101254

So-Yeon Park , Jun-Kyu Lee , Sang-Hyeok Lee , Da-Seul Kim , Ji-Won Jung , Jun Hyuk Kim , Seung-Woon Baek , Seungkwon You , Dong-Youn Hwang , Dong Keun Han

{"title":"Multifunctional vitamin D-incorporated PLGA scaffold with BMP/VEGF-overexpressed tonsil-derived MSC via CRISPR/Cas9 for bone tissue regeneration","authors":"So-Yeon Park , Jun-Kyu Lee , Sang-Hyeok Lee , Da-Seul Kim , Ji-Won Jung , Jun Hyuk Kim , Seung-Woon Baek , Seungkwon You , Dong-Youn Hwang , Dong Keun Han","doi":"10.1016/j.mtbio.2024.101254","DOIUrl":"10.1016/j.mtbio.2024.101254","url":null,"abstract":"<div><p>Guiding endogenous regeneration of bone defects using biomaterials and regenerative medicine is considered an optimal strategy. One of the effective therapeutic approaches involves using transgene-expressed stem cells to treat tissue destruction and replace damaged parts. Among the various gene editing techniques for cells, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is considered as a promising method owing to the increasing therapeutic potential of cells by targeting specific sites. Herein, a vitamin D-incorporated poly(lactic-co-glycolic acid) (PLGA) scaffold with bone morphogenetic protein 2 (BMP2)/vascular endothelial growth factor (VEGF)-overexpressed tonsil-derived MSCs (ToMSCs) via CRISPR/Cas9 was introduced for bone tissue regeneration. The optimized seeding ratio of engineered ToMSCs on the scaffold demonstrated favorable immunomodulatory function, angiogenesis, and osteogenic activity <em>in vitro</em>. The multifunctional scaffold could potentially support stem cell <em>in vivo</em> and induce the transition from M1 to M2 macrophage with magnesium hydroxide and vitamin D. This study highlights the improved synergistic effect of a vitamin D-incorporated PLGA scaffold and a gene-edited ToMSCs for bone tissue engineering and regenerative medicine.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101254"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003156/pdfft?md5=bf243ccb3a040b857f200eee3764c7ba&pid=1-s2.0-S2590006424003156-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional gas-foamed scaffolds decorated with metal phenolic networks for cartilage regeneration 装饰有金属酚醛网络的三维气泡支架用于软骨再生

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-14 DOI: 10.1016/j.mtbio.2024.101249

Yujie Chen , Wei Xu , Zhen Pan , Bohui Li , Xiumei Mo , Yucai Li , Jielin Wang , Yuan Wang , Zhenyuan Wei , Yicheng Chen , Zhaopu Han , Chen Lin , Yu Liu , Xiaojian Ye , Jiangming Yu

{"title":"Three-dimensional gas-foamed scaffolds decorated with metal phenolic networks for cartilage regeneration","authors":"Yujie Chen , Wei Xu , Zhen Pan , Bohui Li , Xiumei Mo , Yucai Li , Jielin Wang , Yuan Wang , Zhenyuan Wei , Yicheng Chen , Zhaopu Han , Chen Lin , Yu Liu , Xiaojian Ye , Jiangming Yu","doi":"10.1016/j.mtbio.2024.101249","DOIUrl":"10.1016/j.mtbio.2024.101249","url":null,"abstract":"<div><p>Inflammation is a major impediment to the healing of cartilage injuries, yet bioactive scaffolds suitable for cartilage repair in inflammatory environments are extremely rare. Herein, we utilized electrospinning to fabricate a two-dimensional nanofiber scaffold (2DS), which was then subjected to gas foaming to obtain a three-dimensional scaffold (3DS). 3DS was modified with metal phenolic networks (MPNs) composed of epigallocatechin gallate (EGCG) and strontium ions (Sr<sup>2+</sup>) to afford a MPNs-modified 3D scaffold (3DS-E). Gas-foamed scaffold exhibited multilayered structure conducive to cellular infiltration and proliferation. Compared to other groups, 3DS-E better preserved chondrocytes under interleukin (IL)-1β induced inflammatory environment, showing less apoptosis of chondrocytes and higher expression of cartilage matrix. Additionally, 3DS-E facilitated the regeneration of more mature cartilage <em>in vivo</em>, reduced cell apoptosis, and decreased the expression of pro-inflammatory cytokines.</p><p>Taken together, 3DS-E may offer an ideal candidate for cartilage regeneration.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101249"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003107/pdfft?md5=ab4c760f50b36efebb811bc7f1572d90&pid=1-s2.0-S2590006424003107-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application trends and strategies of hydrogel delivery systems in intervertebral disc degeneration: A bibliometric review 水凝胶给药系统在椎间盘退变中的应用趋势和策略：文献综述

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-14 DOI: 10.1016/j.mtbio.2024.101251

Junwu Wang , Yu Zhang , Yilong Huang , Zhuowen Hao , Guang Shi , Lanhong Guo , Chunyu Chang , Jingfeng Li

{"title":"Application trends and strategies of hydrogel delivery systems in intervertebral disc degeneration: A bibliometric review","authors":"Junwu Wang , Yu Zhang , Yilong Huang , Zhuowen Hao , Guang Shi , Lanhong Guo , Chunyu Chang , Jingfeng Li","doi":"10.1016/j.mtbio.2024.101251","DOIUrl":"10.1016/j.mtbio.2024.101251","url":null,"abstract":"<div><p>Hydrogels are widely used to explore emerging minimally invasive strategies for intervertebral disc degeneration (IVDD) due to their suitability as drug and cell delivery vehicles. There has been no review of the latest research trends and strategies of hydrogel delivery systems in IVDD for the last decade. In this study, we identify the application trends and strategies in this field through bibliometric analysis, including aspects such as publication years, countries and institutions, authors and publications, and co-occurrence of keywords. The results reveal that the literature in this field has been receiving increasing attention with a trend of growth annually. Subsequently, the hotspots of hydrogels in this field were described and discussed in detail, and we proposed the “four core factors”, hydrogels, cells, cell stimulators, and microenvironmental regulation, required for a multifunctional hydrogel for IVDD. Finally, we discuss the popular and emerging mechanistic strategies of hydrogel therapy for IVDD in terms of five aspects: fundamental pathologic changes in IVDD, counteracting cellular senescence, counteracting cell death, improving organelle function, and replenishing exogenous cells. This study provides a reference and a new perspective for future research in this urgently needed field.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101251"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003120/pdfft?md5=7395ca53b698dbf60b5359e9757ac6ec&pid=1-s2.0-S2590006424003120-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging 利用时间门控生物成像技术对室温磷光进行原位累积光谱信息分析

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-14 DOI: 10.1016/j.mtbio.2024.101238

Yong Ho Cho , Seokho Kim , Tae Kyung Won , Sunki Cho , Dong June Ahn

{"title":"Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging","authors":"Yong Ho Cho , Seokho Kim , Tae Kyung Won , Sunki Cho , Dong June Ahn","doi":"10.1016/j.mtbio.2024.101238","DOIUrl":"10.1016/j.mtbio.2024.101238","url":null,"abstract":"<div><p>This study introduces the time-gated analysis of room-temperature phosphorescence (RTP) for the in-situ analysis of the visible and spectral information of photons. Time-gated analysis is performed using a microscopic system consisting of a spectrometer, which is advantageous for in-situ analysis since it facilitates the real-time measurement of luminescence signal changes. An RTP material hybridized with a DNA aptamer that targets a specific protein enhances the intensity and lifetime of phosphorescence after selective recognition with the target protein. In addition, time-gated analysis allows for the millisecond-scale imaging of phosphorescence signals, excluding autofluorescence, and improves the signal-to-background ratio (SBR) through the accumulation of signals. While collecting the time-gated images and spectra of RTP and autofluorescent materials simultaneously, we develop a method for obtaining phosphorescence signals by means of selective exclusion of autofluorescence signals in simulated or real cell conditions. It is confirmed that the accumulated time-gated analysis can provide ample information about luminescence signals for bioimaging and biosensing applications.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101238"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424002990/pdfft?md5=d4a8408d3840fab5ae74eaeefdb58eb1&pid=1-s2.0-S2590006424002990-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A wireless, battery-free device for electrical neuromodulation of bladder contractions 无需电池的无线膀胱收缩神经调节装置

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-13 DOI: 10.1016/j.mtbio.2024.101233

Jun Li , Guoxian Deng , Xianping Li , Lingxuan Yin , Chunhui Yuan , Wei Shao , Xiaowen Xia , Junan Yan , Jiwei Yao

{"title":"A wireless, battery-free device for electrical neuromodulation of bladder contractions","authors":"Jun Li , Guoxian Deng , Xianping Li , Lingxuan Yin , Chunhui Yuan , Wei Shao , Xiaowen Xia , Junan Yan , Jiwei Yao","doi":"10.1016/j.mtbio.2024.101233","DOIUrl":"10.1016/j.mtbio.2024.101233","url":null,"abstract":"<div><p>Lower urinary tract dysfunction (LUTD) is a prevalent condition characterized by symptoms such as urinary frequency, urgency, incontinence, and difficulty in urination, which can significantly impair patient's quality of life and lead to severe physiological complications. Despite the availability of diverse treatment options, including pharmaceutical and behavioral therapies, these approaches are not without challenges. The objective of this study was to enhance treatment options for LUTD by developing a wireless, battery-free device for managing bladder contractions. We designed and validated a compact, fully implantable, battery-free pulse generator using the magnetic induction coupling mechanism of wireless power transmission. Weighing less than 0.2 g and with a volume of less than 0.1 cubic centimeters, this device enables precise stimulation of muscles or neurons at voltages ranging from 0 to 10 V. Wireless technology allows real-time adjustment of key stimulation parameters such as voltage, duration, frequency, pulse width, and pulse interval. Our findings demonstrate that the device effectively controlled bladder contractions in mice when used to stimulate the Major Pelvic Ganglion (MPG). Additionally, the device successfully managed micturition in mice with bilateral transection of the pudendal nerve. In conclusion, the development of this innovative wireless pulse generator provides a safer and more cost-effective alternative to conventional battery-powered neurostimulators for bladder control, addressing the limitations of such devices. We anticipate that this novel technology will play a pivotal role in the future of electrical stimulation therapies for voiding dysfunctions.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101233"},"PeriodicalIF":8.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424002941/pdfft?md5=17547b73f6791b80aff2b083d1120774&pid=1-s2.0-S2590006424002941-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction 用于板层角膜移植术和组织工程角膜构建的高透明度角膜细胞外基质的制备、物理生化特性分析和蛋白质组学分析

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-13 DOI: 10.1016/j.mtbio.2024.101241

Yoshihide Hashimoto , Jun Negishi , Seiichi Funamoto , Tsuyoshi Kimura , Hisatoshi Kobayashi , Tetsuro Oshika , Akio Kishida

{"title":"Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction","authors":"Yoshihide Hashimoto , Jun Negishi , Seiichi Funamoto , Tsuyoshi Kimura , Hisatoshi Kobayashi , Tetsuro Oshika , Akio Kishida","doi":"10.1016/j.mtbio.2024.101241","DOIUrl":"10.1016/j.mtbio.2024.101241","url":null,"abstract":"<div><p>Corneal opacity and deformation, which often require corneal transplantation for treatment, are among the leading causes of monocular blindness. To restore corneal clarity and integrity, there is a need for an artificial stroma that not only matches the transparency of donated human cornea but also effectively integrates to the corneal tissue. In this study, a transparent decellularized cornea was successfully developed using the high hydrostatic pressure method with processing conditions optimized for corneal decellularization. Biochemical analyses demonstrated the effective removal of cellular components from the transparent decellularized corneas, while preserving collagen and glycosaminoglycans. Proteome analysis also revealed that core matrisome and matrisome-associated proteins remained following decellularization, similar to the composition observed in untreated corneas. The light transmittance of the transparent decellularized corneas was 86.4 ± 1.5 % in the visible region, comparable to that of donated human corneas. No complications, such as angiogenesis, were observed following interlamellar corneal transplantation in rabbits. The grafts were almost imperceptible immediately following surgery and achieved complete transparency within a few days, becoming indistinguishable even under a microscope. The transparent decellularized cornea presented here has promising potential as a material for application in lamellar keratoplasty.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101241"},"PeriodicalIF":8.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003028/pdfft?md5=d265a3c226bcf533ac4f851c0091fdd3&pid=1-s2.0-S2590006424003028-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D radially aligned nanofiber scaffold co-loaded with LL37 mimetic peptide and PDGF-BB for the management of infected chronic wounds 共同载入 LL37 拟态肽和 PDGF-BB 的三维径向排列纳米纤维支架用于治疗感染性慢性伤口

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-12 DOI: 10.1016/j.mtbio.2024.101237

Fei Li , Chuwei Zhang , Xiaoping Zhong , Bo Li , Mengnan Zhang , Wanqian Li , Lifei Zheng , Xinghua Zhu , Shixuan Chen , Yi Zhang

{"title":"A 3D radially aligned nanofiber scaffold co-loaded with LL37 mimetic peptide and PDGF-BB for the management of infected chronic wounds","authors":"Fei Li , Chuwei Zhang , Xiaoping Zhong , Bo Li , Mengnan Zhang , Wanqian Li , Lifei Zheng , Xinghua Zhu , Shixuan Chen , Yi Zhang","doi":"10.1016/j.mtbio.2024.101237","DOIUrl":"10.1016/j.mtbio.2024.101237","url":null,"abstract":"<div><p>Diabetic foot ulcers, pressure ulcers, and bedsores can easily develop into chronic wounds with bacterial infections, complicating wound healing. This work reports a two-step strategy for treating infected chronic wounds. Firstly, LL37 mimetic peptide-W379 peptides were rapidly released to eliminate the bacterial biofilm on the wound. Then, 3D radially aligned nanofiber scaffolds loaded with W379 antimicrobial peptide and PDGF-BB were used to treat the wound to prevent bacterial infection recurrence and promote angiogenesis and granulation tissue regeneration, thereby accelerating wound healing. In the presented study, we found that the combined use of burst and controlled release of W379 antimicrobial peptide effectively clears the bacterial biofilm and prevents the recurrence of bacterial infection. Additionally, we found that the removal of the bacterial biofilm contributed to modulating the local inflammatory response from a pro-inflammatory type to a pro-regenerative type. Furthermore, the use of PDGF-BB significantly promotes neovascularization and granulation tissue regeneration in the wound bed, resulting in accelerating re-epithelialization and wound closure. Our study provides a promising treatment method for the repair of infected chronic wounds.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101237"},"PeriodicalIF":8.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424002989/pdfft?md5=9db1fccfdc71ff755d256982b1bd59fa&pid=1-s2.0-S2590006424002989-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers 与深层脂肪组织相比，来自腹部浅层脂肪组织的脱细胞脂肪源基质具有更强的脂肪生成能力

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-12 DOI: 10.1016/j.mtbio.2024.101235

Xiaomu Ma , Qiang Yue , Su Fu, Chunjun Liu , Jie Luan

{"title":"Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers","authors":"Xiaomu Ma , Qiang Yue , Su Fu, Chunjun Liu , Jie Luan","doi":"10.1016/j.mtbio.2024.101235","DOIUrl":"10.1016/j.mtbio.2024.101235","url":null,"abstract":"<div><p>The adipogenic property of decellularized adipose-derived matrix (DAM) varies widely across reports, making it difficult to make a horizontal comparison between reports and posing challenges for the stable clinical translation of DAM. It is possibly due to differences in donor characteristics, but the exact relationship remains unclear. Despite extensive research on the differences between superficial and deep layers of abdominal subcutaneous fat, a main donor of DAM, little is known about their extracellular matrix (ECM) which is promising in regenerative medicine. In this study, we first confirmed the distinct compositional profiles and adipogenic potential between superficial and deep DAM (S-DAM and D-DAM). Both in vitro and in vivo assays confirmed superior adipogenic induction potential in S-DAM over D-DAM. Total amounts of ECM proteins like collagen and laminin were similar, however, the predominant types differed, with collagen I dominating S-DAM and collagen XIV prevailing in D-DAM. S-DAM was enriched with mitochondrial and immunological proteins, whereas D-DAM featured more neuronal, vascular, muscular, and endocrine-related proteins. More proteins involved in mRNA processing were found in D-DAM, with Protein-Protein Interaction (PPI) analysis revealing HNRNPA2B1, HNRNPA1, and HNRNPC as the most tightly interacting members. These findings not only deepen our comprehension of the structural and functional heterogeneity of adipose tissues but also become one of the reason for the large variability between batches of DAM products, providing guidance for constructing more efficient and stable bio-scaffolds.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101235"},"PeriodicalIF":8.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424002965/pdfft?md5=a87afc1211964ece3544b154f2dbd0bf&pid=1-s2.0-S2590006424002965-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo targeted-imaging of mitochondrial acidification in an aristolochic acid I-induced nephrotoxicity mouse model by a fluorescent/photoacoustic bimodal probe 利用荧光/光声双模探针对马兜铃酸 I 诱导的肾毒性小鼠模型中的线粒体酸化进行体内靶向成像

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-09-11 DOI: 10.1016/j.mtbio.2024.101240

Li Xu , Li Chen , Hongwen Liu , Xingwang Chen , Shenghang Zhang

{"title":"In vivo targeted-imaging of mitochondrial acidification in an aristolochic acid I-induced nephrotoxicity mouse model by a fluorescent/photoacoustic bimodal probe","authors":"Li Xu , Li Chen , Hongwen Liu , Xingwang Chen , Shenghang Zhang","doi":"10.1016/j.mtbio.2024.101240","DOIUrl":"10.1016/j.mtbio.2024.101240","url":null,"abstract":"<div><p>Aristolochic acid I (AAI), a natural compound in aristolochia type Chinese medicinal herb, is generally acknowledged to have nephrotoxicity, which may be associated with mitophagy. Mitophagy is a cellular process with important functions that drive AAI-induced renal injury. Mitochondrial pH is currently measured by fluorescent probes in cell culture, but existing probes do not allow for in situ imaging of AAI-induced mitophagy in vivo. We developed a ratiometric fluorescent/PA dual-modal probe with a silicon rhodamine fluorophore and a pH-sensitive hemicyanine dye covalently linked via a short chain to obtain a FRET type probe. The probe was used to measure AAI-mediated mitochondrial acidification in live cells and in vivo. The Förster resonance energy transfer (FRET)-mediated ratiometric and bimodal method can efficiently eliminate signal variability associated with the commonly used one-emission and single detection mode by ratiometric two channels of the donor and acceptor. The probe has good water-solubility and low molecular weight with two positively charged, facilitating its precise targeting into renal mitochondria, where the fluorescent/PA changes in response to mitochondrial acidification, enabling dynamic and semi-quantitative mapping of subtle changes in mitochondrial pH in AAI-induced nephrotoxicity mouse model for the first time. Also, the joint use of L-carnitine could mitigate the mitophagy in AAI-induced nephrotoxicity.</p></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"28 ","pages":"Article 101240"},"PeriodicalIF":8.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590006424003016/pdfft?md5=06839d4d87b8973e74ad4bf8992f6a8a&pid=1-s2.0-S2590006424003016-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0