Materials Today Bio最新文献_第9页

Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization. 贻贝诱导的同轴静电纺纳米纤维膜通过增强巨噬细胞M2极化促进口腔粘膜无疤痕修复。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-30 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103272

Junpeng Chen, Jinpeng Jiang, Wenyi Shen, Bingjie Xu, Haina Miao, Yangxi Cheng, Yi Zheng, Huiyong Zhu, Dan Yu

{"title":"Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization.","authors":"Junpeng Chen, Jinpeng Jiang, Wenyi Shen, Bingjie Xu, Haina Miao, Yangxi Cheng, Yi Zheng, Huiyong Zhu, Dan Yu","doi":"10.1016/j.mtbio.2026.103272","DOIUrl":"10.1016/j.mtbio.2026.103272","url":null,"abstract":"The repair of oral mucosal defects is often hindered by the failure of traditional materials to maintain adhesion in humid, dynamic environments. Inspired by the wet-adhesion properties of mussels, we developed a biomimetic self-adhesive nanofibrous membrane via coaxial electrospinning. This membrane utilizes a polycaprolactone (PCL) core for mechanical strength and a dopamine (DA)-modified gelatin methacrylate (GelMA) shell for biocompatibility and adhesion. Experimental results demonstrate that the membrane possesses excellent mechanical properties, with a tensile strength of 2.52 MPa and a swelling degree of 554.6%. The incorporation of 2% DA significantly enhanced wet-adhesive strength to 30.53 kPa, allowing for stable adhesion to oral tissues for over 24 h. In vitro experiments demonstrated good cytocompatibility and promoted fibroblast adhesion and proliferation. In vivo studies using murine skin and rat oral mucosal defect models showed accelerated wound closure, reduced scar formation, and improved tissue regeneration. Immunological analyses revealed that the DA-modified nanofiber membrane modulated the local immune microenvironment by promoting macrophage polarization toward the anti-inflammatory M2 phenotype. Transcriptomic analysis and subsequent molecular validation identified activation of the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway as a key mechanism underlying this immunoregulatory effect. These results demonstrate that the mussel-inspired coaxial nanofiber membrane provides stable wet adhesion and actively regulates immune responses to promote scarless healing of oral mucosal defects, highlighting its potential as a functional biomaterial for soft tissue repair.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103272"},"PeriodicalIF":11.0,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-targeted cuproptosis-driven nanoplatform for synergistic photothermal/chemodynamic therapy and systemic antitumor immunotherapy. 线粒体靶向铜胞嘧啶驱动的纳米平台，用于协同光热/化学动力学治疗和全身抗肿瘤免疫治疗。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-30 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103303

Hui Zhang, Yuting Lu, Jingchun Wang, Yikai Ma, Yuanjin Sun, Shengzhong Rong, Xu Zhu, Yingxue Jin

{"title":"Mitochondria-targeted cuproptosis-driven nanoplatform for synergistic photothermal/chemodynamic therapy and systemic antitumor immunotherapy.","authors":"Hui Zhang, Yuting Lu, Jingchun Wang, Yikai Ma, Yuanjin Sun, Shengzhong Rong, Xu Zhu, Yingxue Jin","doi":"10.1016/j.mtbio.2026.103303","DOIUrl":"10.1016/j.mtbio.2026.103303","url":null,"abstract":"Cuproptosis, a recently identified copper-dependent regulated cell death pathway driven by mitochondrial lipoylated protein aggregation, holds considerable promise for cancer therapy. Its clinical translation is constrained by off-target toxicity of free copper ions, inadequate mitochondria-targeted delivery and the immunosuppressive tumor microenvironment. We developed a hyaluronic acid-camouflaged, pH-responsive nanoplatform HACCR for mitochondria-targeted, cuproptosis-driven synergistic therapy and systemic antitumor immunity. The nanoplatform core relies on self-assembled architecture via π-π stacking between copper-metformin carbon dots and immune adjuvant R837, achieving high payload efficiencies 43.6% for CuMCDs and 35.2% for R837. CuMCDs synthesized via one-pot hydrothermal synthesis exhibit intrinsic mitochondria-targeting properties, facilitating precise copper delivery to cuproptosis initiation sites and amplifying pathway activation. They display efficient photothermal conversion under 808 nm laser irradiation, directly inducing tumor cell death while enhancing cuproptosis, chemodynamic therapy and immunogenic cell death. The hyaluronic acid-cinnamaldehyde micelle shell enables CD44-mediated tumor targeting and acidic tumor microenvironment-responsive payload release to mitigate off-target effects. In vitro and in vivo evaluations confirm effective cuproptosis induction, immunosuppressive tumor microenvironment reversal and enhanced dendritic cell maturation and T cell infiltration. Combined with anti-PD-L1 checkpoint blockade, HACCR achieves robust primary tumor eradication and distant metastasis inhibition with favorable systemic biosafety. This work establishes a versatile nanoplatform for cuproptosis-based synergistic cancer immunotherapy, offering insights for clinical translation of copper-dependent cell death strategies.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103303"},"PeriodicalIF":11.0,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13253154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sprayable asymmetric hydrogel prevents postoperative pleural adhesions via physical and biological dual therapy. 非对称水凝胶喷雾通过物理和生物双重治疗预防术后胸膜粘连。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-30 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103297

Ni Li, Zherui Shao, Nuo Cheng, Yongbin Wang, Bin Wu, Bingchuan Hu, Qinning Wang, Yanggang Dan, Yuwei Zhou, Kanghao Zhu, Renyuan Xiao, Caoyun Ju, Cong Wang, Dawei Zheng

{"title":"Sprayable asymmetric hydrogel prevents postoperative pleural adhesions via physical and biological dual therapy.","authors":"Ni Li, Zherui Shao, Nuo Cheng, Yongbin Wang, Bin Wu, Bingchuan Hu, Qinning Wang, Yanggang Dan, Yuwei Zhou, Kanghao Zhu, Renyuan Xiao, Caoyun Ju, Cong Wang, Dawei Zheng","doi":"10.1016/j.mtbio.2026.103297","DOIUrl":"10.1016/j.mtbio.2026.103297","url":null,"abstract":"Postoperative pleural adhesions represent a prevalent complication that causes chronic pain, impaired pulmonary function, and escalated reoperation risks. Current preventions strategies remain limited by inadequate multifunctionality and an underestimated fibro-inflammatory microenvironment. Here, we report a powder-based asymmetric adhesive hydrogel integrated with dual-drug liposomes for adhesion prophylaxis. Upon contact with body fluids, the powder rapidly forms an asymmetric adhesive hydrogel within 20 s, exhibiting strong wet-tissue adhesion (∼26 kPa), effective hemostasis, sealing, antibacterial and ROS scavenging capabilities, which display superiority over the commercial absorbable adhesion barrier (INTERCEED®). Embedded liposomes co-loaded with miR-124-3p (an identified critical miRNA from adhesive tissues) and simvastatin enable sustained release, synergistically disrupting the fibro-inflammatory cascade-miR-124-3p attenuates fibrosis via targeting TGFBR1 while simvastatin suppresses pro-inflammatory signaling. In pleural injury rats, the system markedly reduces adhesion, inflammation and fibrosis, while promoting alveolar repair. This study combines an easy-to-apply hydrogel with rationally designed combination therapy, offering a promising strategy for preventing postoperative adhesions with broad potential for translation to other fibrotic disorders.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103297"},"PeriodicalIF":11.0,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13255078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable acoustic rotation for deep biophysical phenotyping of preclinical Alzheimer's disease. 可调声学旋转用于临床前阿尔茨海默病的深层生物物理表型。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-30 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103306

Yuxin Wang, Yue Quan, Shizheng Zhou, Yinning Zhou

{"title":"Tunable acoustic rotation for deep biophysical phenotyping of preclinical Alzheimer's disease.","authors":"Yuxin Wang, Yue Quan, Shizheng Zhou, Yinning Zhou","doi":"10.1016/j.mtbio.2026.103306","DOIUrl":"10.1016/j.mtbio.2026.103306","url":null,"abstract":"Despite advances in imaging and biomarker-based approaches, early diagnosis of Alzheimer's disease (AD) at single-cell resolution remains challenging. Biophysical signals at single-cell scale such as calcium dysregulation and reduced membrane fluidity may serve as early markers of AD, yet these weak signals are difficult to capture. Here, we characterized both phenomena using sensitive optical readouts that were enhanced by acoustic-induced cell rotation. The photobleaching half-life of the calcium indicator fluorescence signal and the half-recovery time of the readout used to characterize membrane fluidity both varied with the acoustic frequency used to induce cell rotation. Signal enhancement peaked within an intermediate acoustic-frequency window and decreased at both lower and higher frequencies, producing a bandpass-like response. This frequency-dependent behavior guided the selection of optimal operating frequencies for subsequent measurements. We then established an Amyloid-β (Aβ)-induced in vitro cellular model and used multidimensional spatial, temporal, and frequency-domain features to evaluate Aβ-induced cellular stress states. The acoustic-rotation approach distinguished early Aβ-induced cellular stress from untreated controls with 99.0% accuracy and classified five cellular states defined by Aβ exposure duration with 94.8% accuracy. By actively enhancing weak single-cell functional signals, this frequency-dependent acoustic strategy provides a generalizable approach for live-cell optical phenotyping and may support future preclinical studies of AD-related cellular stress.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103306"},"PeriodicalIF":11.0,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148198992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic hydrogel-mediated periodic stimulation enhances mesenchymal stem cell small extracellular vesicle release. 磁水凝胶介导的周期性刺激促进间充质干细胞细胞外小泡释放。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103285

Chen Wang, Ye Chen, Tianhao Yan, Shuxin Zhang, Xiaoru Li, Shangquan Wu, Qingchuan Zhang

{"title":"Magnetic hydrogel-mediated periodic stimulation enhances mesenchymal stem cell small extracellular vesicle release.","authors":"Chen Wang, Ye Chen, Tianhao Yan, Shuxin Zhang, Xiaoru Li, Shangquan Wu, Qingchuan Zhang","doi":"10.1016/j.mtbio.2026.103285","DOIUrl":"10.1016/j.mtbio.2026.103285","url":null,"abstract":"Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) are regarded as important \"cell-free\" therapeutic carriers due to their potential for immune regulation and tissue repair. However, their large-scale application is constrained by bottlenecks such as low secretion yields under conventional culture conditions. This study established a magnetically controlled dynamic mechanical stimulation platform to enhance MSC-sEVs production. Superparamagnetic Fe3O4 nanoparticles were embedded into polyacrylamide to form magnetic composite hydrogels. An variable magnetic field induced reversible deformation of the hydrogel, thereby applying periodic mechanical loading to adherent cells. Results demonstrate that, compared to conventional plastic substrates, dynamic loading on MSCs significantly increases sEVs secretion (up to approximately 5-fold) without altering typical vesicle morphology or size distribution. Mechanistic studies indicate that this stimulus induces upregulation of the mechanosensitive channel Piezo1 and the nuclear YAP signaling pathway, thereby promoting sEVs release. Inhibition of Piezo1 or YAP attenuated the enhanced production effect, supporting a dual-pathway synergistic model where \"Piezo1-Ca2+ promotes release, while YAP promotes multivesicular bodies (MVB) generation.\" This platform offers an economical, simple, and promising solution for efficient and controllable sEVs production.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103285"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intelligent multimodal-energy-driven piezoelectric antibacterial platforms: From structural control to system-level diagnosis. 智能多模态能量驱动压电抗菌平台：从结构控制到系统级诊断。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103299

Jiaqi Liu, Jun Li, Huiyan Sun, Jungmok Seo, Yanmei Zhang, Yunlong Yu

{"title":"Intelligent multimodal-energy-driven piezoelectric antibacterial platforms: From structural control to system-level diagnosis.","authors":"Jiaqi Liu, Jun Li, Huiyan Sun, Jungmok Seo, Yanmei Zhang, Yunlong Yu","doi":"10.1016/j.mtbio.2026.103299","DOIUrl":"10.1016/j.mtbio.2026.103299","url":null,"abstract":"Piezocatalysis offers a non-antibiotic, physical-chemical approach to address the challenge of bacterial infection resistance, but its reliance on single energy sources and empirical design limits its potential. This review focuses on intelligent piezoelectric antibacterial platforms powered by multimodal energy and enhanced by artificial intelligence, systematically summarizing the latest research progress in the field. First, based on an in-depth analysis of the limitations of traditional antibacterial strategies, the evolutionary trajectory of piezoelectric technology from single-energy to multimodal synergistic driving is clarified. Second, we systematically explain the piezocatalytic antibacterial mechanism and reveal its synergistic enhancement with other antibacterial components under multiphysical fields, highlighting their comprehensive advantages in improving antibacterial efficacy and achieving precise spatiotemporal control. Furthermore, the synergistic regulatory effects of key structural parameters on the piezoelectric properties and antibacterial activity of materials are thoroughly analyzed. Additionally, typical application cases of such intelligent platforms in cutting-edge scenarios, such as smart wound management, functional anti-infection implant coatings, and precise intervention for localized infections in the lungs and oral cavity, are systematically reviewed. Finally, critical challenges related to material stability, biosafety, and scalable production are discussed, and future research directions empowered by AI are prospected. This review establishes an interdisciplinary theoretical framework and extensible technical pathway for constructing adaptive piezoelectric antibacterial platforms.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103299"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13253188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic methacrylated hyaluronic acid microspheres incorporating MnO₂ and exosomes for antioxidant defense and inflammation regulation in osteoarthritis. 含MnO2和外泌体的微流体甲基丙烯酸透明质酸微球对骨关节炎的抗氧化防御和炎症调节。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103300

Jianxiang Teng, Jiazhao Pan, Pengcheng Yan, Tianqi Zhou, Zihao Zou, Xiaolin Shu, Hao Liu, Zhuoran Zhang, Sheng Zhou, Qiuhan Chen, Dandan Shen, Xing Zhao, Xiaobin Tian, Long Yang

{"title":"Microfluidic methacrylated hyaluronic acid microspheres incorporating MnO2 and exosomes for antioxidant defense and inflammation regulation in osteoarthritis.","authors":"Jianxiang Teng, Jiazhao Pan, Pengcheng Yan, Tianqi Zhou, Zihao Zou, Xiaolin Shu, Hao Liu, Zhuoran Zhang, Sheng Zhou, Qiuhan Chen, Dandan Shen, Xing Zhao, Xiaobin Tian, Long Yang","doi":"10.1016/j.mtbio.2026.103300","DOIUrl":"10.1016/j.mtbio.2026.103300","url":null,"abstract":"Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degeneration, chronic inflammation, and oxidative stress-induced mitochondrial dysfunction. Herein, we engineered an integrated therapeutic platform by encapsulating mesenchymal stem cell-derived exosomes (Exo) and manganese dioxide (MnO2) nanozymes into methacrylated hyaluronic acid (HAMA) hydrogel microspheres (HAMA-Exo-MnO2) via microfluidic fabrication. Systematic in vitro and in vivo evaluations assessed the microspheres' anti-inflammatory, antioxidant, and chondrogenic regenerative properties. In vitro experiments demonstrated that HAMA-Exo-MnO2 microspheres effectively attenuated OA-like pathological changes in chondrocytes, remodeled the inflammatory microenvironment, and scavenged reactive oxygen species (ROS). In a rat OA model induced by destabilization of the medial meniscus (DMM), intra-articular injection of HAMA-Exo-MnO2 microspheres ameliorated disease progression, enhanced articular cartilage regeneration, and restored joint function. Transcriptomic sequencing and subsequent validation in cocultured OA chondrocytes revealed significant upregulation of ALDH3A1, which mitigated oxidative stress, activated the NRF2 signaling axis, promoted downstream antioxidant gene expression, and restored mitochondrial electron transport chain activity. In summary, this study highlights that the engineered HAMA hydrogel microspheres provide a multifunctional therapeutic strategy for OA treatment, integrating immune microenvironment modulation, cartilage regeneration, and antioxidant protection.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103300"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Notch signaling-targeted biomimetic hydrogel constructs an anti-inflammatory "flood-control" system to alleviate pyroptosis for intervertebral disc degeneration treatment. Notch信号靶向仿生水凝胶构建抗炎“洪水控制”系统，减轻椎间盘退变治疗中的焦下垂。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103298

Rui Zhang, Xiaoyu Zhang, Jiangbo Guo, Yilin Yang, Junjie Niu, Hao Shen, Yi Zheng, Jiaqi Wang, Suhao Wang, Li Ni, Huilin Yang, Qin Shi

{"title":"Notch signaling-targeted biomimetic hydrogel constructs an anti-inflammatory \"flood-control\" system to alleviate pyroptosis for intervertebral disc degeneration treatment.","authors":"Rui Zhang, Xiaoyu Zhang, Jiangbo Guo, Yilin Yang, Junjie Niu, Hao Shen, Yi Zheng, Jiaqi Wang, Suhao Wang, Li Ni, Huilin Yang, Qin Shi","doi":"10.1016/j.mtbio.2026.103298","DOIUrl":"10.1016/j.mtbio.2026.103298","url":null,"abstract":"Intervertebral disc degeneration (IVDD) is the major pathological cause of low back pain and global disability. Its chronic and persistent inflammatory microenvironment, resembling a flowing flood to erode the ecosystems relentlessly, disrupts the intervertebral disc homeostasis. In the present study, we first verified that Notch signaling is negatively correlated with inflammation in IVDD samples. Meanwhile, we identified a natural flavonoid hesperetin (Hes) as a potential agonist of Notch1 by molecular docking predicting. Herein, we developed an EV-inspired bioactive reservoir system, in which the natural extracts hes was encapsulated into liposomes (Hes@lipo) and embedded within a gelatin methacryloyl (GM) hydrogel (GM/Hes@lipo)to achieve sustained release. By mimicking the extracellular matrix (ECM)-like microenvironment of the intervertebral disc (IVD), GM/Hes@lipo provided physiological environment for nucleus pulposus cells (NPCs) adhesion and growth maintenance. Furthermore, by activating Notch signaling, GM/Hes@lipo effectively restored the dynamic balance between pro- and anti-inflammatory cytokines and maintained ECM metabolic homeostasis, attenuating NOD-like receptor signaling, thereby inhibiting NPC pyroptosis. Based on these aspects, GM/Hes@lipo relieved the IVDD process, maintained annulus fibrosus integrity, and effectively alleviated discogenic pain after the platform was administrated into the intervertebral disc of the rats. Collectively, as an anti-inflammatory \"flood-control\" system, the Notch signaling-targeted biomimetic hydrogel presents a biomimetic-natural compound combinatorial strategy offering promising therapy for mitigating IVDD progression.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103298"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13254983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hesperetin-black phosphorus nanosheets targeting TAMs induce ferroptosis and reverse protumoral polarization to remodel the immune microenvironment. 以tam为靶点的橙皮苷黑磷纳米片诱导铁凋亡，逆转肿瘤极化，重塑免疫微环境。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103304

Liang Feng, Huan Wang, Zuodi Liang, Ying Tian, Yang Guo, Zhuo Li

{"title":"Hesperetin-black phosphorus nanosheets targeting TAMs induce ferroptosis and reverse protumoral polarization to remodel the immune microenvironment.","authors":"Liang Feng, Huan Wang, Zuodi Liang, Ying Tian, Yang Guo, Zhuo Li","doi":"10.1016/j.mtbio.2026.103304","DOIUrl":"10.1016/j.mtbio.2026.103304","url":null,"abstract":"This study focused on developing a novel nanodelivery system, BP-PEG-S2P@H, with hesperetin-loaded black phosphorus nanosheets to target tumor-associated macrophages (TAMs) in triple-negative breast cancer (TNBC). Through RNA sequencing and proteomic analyses, CEBPG upregulation was observed in TAMs, potentially controlling the SLC7A11-mediated ferroptosis pathway. In vitro and in vivo experiments, including FerroOrange and malondialdehyde assays, demonstrated the induction of ferroptosis by BP-PEG-S2P@H. The nanoplatform facilitated the transition of TAMs from the M2 to the M1 subtype, enhancing IL-12 secretion while reducing IL-10 levels. Functional analyses confirmed the reversal of the pro-migratory TAM phenotype and inhibition of tumor cell invasion. In vivo imaging validated efficient tumor targeting, with immunohistochemical staining revealing decreased CEBPG/SLC7A11 expression in tumors, reduced macrophage infiltration, and enhanced CD8+ T cell activation, promoting the efficacy of PD-L1 immunotherapy. Biosafety assessments indicated no apparent toxicity, supporting the potential of the BP-PEG-S2P@H nanoplatform for TAM-targeted nano-immunotherapy in TNBC.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103304"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D HucMSCs derived extracellular vesicles enhanced therapeutic efficacy in treating intrauterine adhesions via BECN1 delivery. 三维HucMSCs来源的细胞外囊泡增强了BECN1输注治疗宫内粘连的疗效。

IF 11 1区医学

Materials Today Bio Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mtbio.2026.103276

Juan Peng, Juan Zhang, Qingzhao Li, Weijun Liu, Wenda Zou, Liyu Zhu, Qianyin Zhou, Dan Liu, Man Jia, Hui Li

{"title":"3D HucMSCs derived extracellular vesicles enhanced therapeutic efficacy in treating intrauterine adhesions via BECN1 delivery.","authors":"Juan Peng, Juan Zhang, Qingzhao Li, Weijun Liu, Wenda Zou, Liyu Zhu, Qianyin Zhou, Dan Liu, Man Jia, Hui Li","doi":"10.1016/j.mtbio.2026.103276","DOIUrl":"10.1016/j.mtbio.2026.103276","url":null,"abstract":"Intrauterine adhesion (IUA) is a significant cause of female infertility and a critical public health concern among women of childbearing age. Emerging evidence has highlighted the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (EVs) in IUA treatment. However, conventional two-dimensional (2D) culture systems impose constraints on the yield and biological activity of EVs. In this study, we employed a three-dimensional (3D) culture platform based on gelatin methacrylate (GelMA) microspheres to culture human umbilical cord mesenchymal stem cells and isolate MSC-EVs. Comparative analysis demonstrated that 3D-EVs exhibited significantly superior therapeutic effects on restoring the proliferation, migration, viability, and alleviating fibrosis of mifepristone-injured human endometrial stromal cells (hESCs) compared with 2D-EVs in vitro. In IUA animal models, in situ treatment with GelMA-encapsulated 3D-EVs exhibited remarkable regenerative capabilities. The treatment effectively rejuvenated endometrial structure including mucosal thickness and glandular density, ameliorated endometrial fibrosis, and ultimately improved reproductive outcomes. Results from proteomic profiling and functional verification assays revealed that elevated expression of BECN1 in 3D-EVs contributes to the enhanced therapeutic effect of 3D-EVs on injured hESCs. This study establishes 3D culture-derived EVs as an innovative cell-free therapeutic approach for endometrial regeneration, addressing both the limitations of traditional EVs production methods and the clinical need for effective IUA treatments. Our findings provide a foundation for advanced regenerative strategies targeting endometrial pathologies.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"38 ","pages":"103276"},"PeriodicalIF":11.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0