Materials Today Bio最新文献

{"title":"Perspectives on the use of decellularized/devitalized and lyophilized human perinatal tissues for bone repair: Advantages and remaining challenges","authors":"Lauriana Solecki ,&nbsp;Mathilde Fenelon ,&nbsp;Halima Kerdjoudj ,&nbsp;Roberta Di Pietro ,&nbsp;Gianmarco Stati ,&nbsp;Camille Gaudet ,&nbsp;Eugenie Bertin ,&nbsp;Jeremie Nallet ,&nbsp;Aurélien Louvrier ,&nbsp;Thomas Gualdi ,&nbsp;Jessica Schiavi-Tritz ,&nbsp;Florelle Gindraux","doi":"10.1016/j.mtbio.2024.101364","DOIUrl":"10.1016/j.mtbio.2024.101364","url":null,"abstract":"<div><div>Human amniotic membrane (hAM) has been extensively used for several decades as a bioactive scaffold for regenerative medicine. In its cryopreserved form—one of the main storage formats—the presence of viable cells has often been questioned. Furthermore, there is little published evidence of the role of endogenous amniotic cells from cryopreserved hAM in tissue repair.</div><div>Some technologies, often patented and combined, have facilitated the use of hAM. Decellularization and devitalization processes have been developed to ensure its safety and prevent immune rejection. Lyophilization and dehydration methods have had a significant impact on clinical practices by enabling storage at room temperature in the operating room and making handling and cutting easier. Consequently, the commercialization of hAM has expanded, initially in the USA, and now in Europe.</div><div>In the last decade, there has been growing interest in new perinatal tissues in clinical medicine. Similar processes have been adapted for these tissues to prevent immune or inflammatory reactions, and to improve storage and make them easier to use. For example, in the USA, many products marketed for wound healing undergo lyophilization, sometimes in combination with decellularization.</div><div>Given our expertise, we wanted to highlight the potential of decellularized/devitalized and lyophilized perinatal tissues in regenerative medicine, particularly for bone repair. In this opinion paper, we discuss why these tissues represent the future of regenerative medicine, their potential drawbacks and strategies to overcome these challenges.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101364"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced hydrogel loading of quercetin-loaded hollow mesoporous cerium dioxide nanoparticles for skin flap survival","authors":"Xiangjun Liu ,&nbsp;Yikun Ju ,&nbsp;Pu Yang ,&nbsp;Naisi Shen ,&nbsp;Yunyuan Shao ,&nbsp;Anqi Yang ,&nbsp;Rui Wu ,&nbsp;Lanjie Lei ,&nbsp;Bairong Fang","doi":"10.1016/j.mtbio.2024.101432","DOIUrl":"10.1016/j.mtbio.2024.101432","url":null,"abstract":"<div><div>Flap techniques are indispensable in modern surgery because of their role in repairing tissue defects and restoring function. Ischemia-reperfusion and oxidative stress-induced injuries are the main causes of flap failure. Oxidative stress exacerbates cell damage through the accumulation of reactive oxygen species (ROS), thereby affecting flap function and survival. Effective management of these factors is essential for improving flap survival and post-operative recovery. In this study, we utilized hollow mesoporous cerium dioxide nanoparticles loaded with quercetin, which were later loaded into a light-cured double cross-linked hydrogel (HQu@BC) and injected into the flap site to activate macrophage reprogramming to maintain local ROS homeostasis and reduce inflammation. Quercetin scavenges ROS and reduces mitochondrial oxidative stress due to its intrinsic reducing structures such as catechols, carbon-carbon double bonds, and hydroxyl synergistic mesoporous cerium dioxide nanoparticles, and inhibits inflammation by suppressing M1 macrophage polarization. This system continuously regulates ROS levels, kills bacteria and ultimately reduces inflammation, thereby creating a favorable microenvironment for flap survival. This innovative injectable composite nanoparticle hydrogel material has anti-inflammatory, antioxidant, antimicrobial, and healing-promoting properties, providing a new approach to improving the success of flap surgery.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101432"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal-organic framework (MOF)-bioactive glass (BG) systems for biomedical applications - A review","authors":"Andrada-Ioana Damian-Buda ,&nbsp;Nariman Alipanah ,&nbsp;Faina Bider ,&nbsp;Orhan Sisman ,&nbsp;Zuzana Neščáková ,&nbsp;Aldo R. Boccaccini","doi":"10.1016/j.mtbio.2024.101413","DOIUrl":"10.1016/j.mtbio.2024.101413","url":null,"abstract":"<div><div>In recent years, metal-organic frameworks (MOFs) have emerged as promising materials for biomedical applications, owing to their superior chemical versatility, unique textural properties and enhanced mechanical properties. However, their fast and uncontrolled degradation, together with the reduced bioactivity have restricted their clinical potential. To overcome these limitations, MOFs can be synergistically combined with other materials, such as bioactive glasses (BGs), known for their bioactivity and therapeutic ion releasing capabilities. Besides comparing MOFs and BGs, this review aims to present the latest achievements of different MOFs/BGs materials, with a particular focus on their complementary and synergistic properties. Key findings show that combining MOFs and BGs enables the development of composite materials with superior physicochemical and biological properties. Moreover, by choosing appropriate processing techniques, BGs and MOFs can be fabricated as scaffolds or coatings with fast mineralization ability and high corrosion resistance. In addition, incorporation of MOFs/BGs in hydrogels improves mechanical stability, bioactivity and antibacterial properties, while maintaining biocompatibility. The mechanisms behind the antibacterial properties, likely coming from the release of metal ions and organic ligands, are also discussed. Overall, this review highlights the current research directions and emerging trends in the synergistic use of MOFs and BGs for biomedical applications, which represents a novel strategy for developing a new family of advanced therapeutic materials.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101413"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional biosynthesized magnetosome for multimodal imaging and combined therapy of tumor","authors":"Xiaoqing Han ,&nbsp;Xingbo Wang ,&nbsp;Jiao Yan ,&nbsp;Panpan Song ,&nbsp;Yanjing Wang ,&nbsp;Yaqing Kang ,&nbsp;Abdur Rauf ,&nbsp;Haiyuan Zhang","doi":"10.1016/j.mtbio.2024.101429","DOIUrl":"10.1016/j.mtbio.2024.101429","url":null,"abstract":"<div><div>The large recruitment of tumor-associated macrophages and low exposure of tumor-associated antigens in tumor microenvironment have severely suppress the efficacy of anti-tumor immunotherapy. Herein, biosynthesized magnetosome (Mag) from bacteria was loaded with photothermal/photodynamic agent/near infrared (NIR) fluorescence dye (IR780) and further modified with lipid-PEG-c(RGDyK) through biomembrane, forming _IMag^RGD for fluorescence imaging, magnetic resonance imaging, immunotherapy and photodynamic/photothermal therapy. After intravenous injection into B16F10 tumor-bearing mice, _IMag^RGD could efficiently accumulate in tumor tissues based on near infrared (NIR) fluorescence and magnetic resonance dual-modality imaging, and repolarize tumor-associated macrophages (TAMs) from M2 phenotype to M1 phenotype, significantly improving the effect of tumor immunotherapy. Moreover, photothermal and photodynamic effect of IR780 could kill tumor cells and elicit immunogenic cell death to mediate anti-tumor immunity, promoting dendritic cells (DCs) maturation and then activating specific effector T cells to further eliminate tumor cells. This study provides a new approach for reversing the activity of tumor immunosuppressive microenvironment and strengthening the efficiency of tumor photoimmunotherapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101429"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide platform for 3D-printed Ti implants with synergistic antibacterial and osteogenic functions to enhance osseointegration","authors":"Chenying Cui ,&nbsp;Yifan Zhao ,&nbsp;Jingyu Yan ,&nbsp;Ziyang Bai ,&nbsp;Guning Wang ,&nbsp;Yingyu Liu ,&nbsp;Yurong Xu ,&nbsp;Lihong Zhou ,&nbsp;Kaifang Zhang ,&nbsp;Yanling Mi ,&nbsp;Binbin Zhang ,&nbsp;Xiuping Wu ,&nbsp;Bing Li","doi":"10.1016/j.mtbio.2024.101430","DOIUrl":"10.1016/j.mtbio.2024.101430","url":null,"abstract":"<div><div>Bone defects caused by trauma, infection, or tumors present a major clinical challenge. Titanium (Ti) implants are widely used due to their excellent mechanical properties and biocompatibility; however, their high elastic modulus, low surface bioactivity, and susceptibility to infection hinder osseointegration and increase failure rates. There is an increasing demand for implants that can resist bacterial infection while promoting osseointegration. In this study, we developed a peptide platform to engineer a multifunctional 3D-printed Ti implant (3DTi) modified with a fusion peptide composed of minTBP-1 (targeting peptide), KR-12 (antibacterial peptide), and GFOGER (adhesion peptide), termed 3DTi-NFP. This design enables specific targeting, localized delivery, prevention of peptide release into circulation, and functional integrity through linker retention. In both in vitro and in vivo infected bone defect models, 3DTi-NFP implants demonstrated excellent biocompatibility and achieved over 90 % bactericidal efficiency against <em>S. aureus</em> and <em>E</em>. <em>coli</em>. The implants reduced bacterial colonization while enhancing adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells (BMSCs), significantly upregulating osteogenic genes and protein expression. Transcriptome sequencing further explored the molecular mechanisms underlying the synergistic effects of 3DTi-NFP, revealing activation of the focal adhesion and PI3K-Akt signaling pathways-key contributors to cell adhesion, matrix formation, and new bone formation. Overall, this study provides a promising strategy to improve the long-term success of Ti-based implants, with significant potential for tissue regeneration and clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101430"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water triggered injectable polylactic acid hydrogel based on zwitterionic sulfobetaine modification for incompressible bleeding and tissue anti-adhesion","authors":"Xinran Yang ,&nbsp;Xiudan Wang ,&nbsp;Lizong Tang ,&nbsp;Zhiguang Sun ,&nbsp;Xing Gao ,&nbsp;Yanmei Zhao ,&nbsp;Shike Hou ,&nbsp;Jie Shi ,&nbsp;Qi Lv","doi":"10.1016/j.mtbio.2024.101431","DOIUrl":"10.1016/j.mtbio.2024.101431","url":null,"abstract":"<div><div>Massive blood loss is the main cause of prehospital trauma-related death, the development of rapid and effective hemostatic materials is imminent. Injectable hydrogels have the advantages of covering irregular bleeding sites and quickly closing the wound. However, its inherent viscosity can easily precipitate tissue adhesion <em>in vivo</em> and other complications. Based on the anti-protein properties of zwitterion and our previous work about <em>in situ</em> hemostatic/anti-adhesion hydrogel material, we have synthesized a series of injectable hydrogel composed of sulfobetaine-modified polylactic acid (PLA) and gelatin (Gel). These hydrogels could form a smooth film structure by simple water triggering, thereby conferring anti-adhesive properties. We visualized the changes in surface hydrophobicity using fluorescent probes and demonstrated tissue adhesion, rapid hydrophobic interface response, as well as rapid hemostasis for incompressible wounds through <em>in vivo</em> and <em>in vitro</em> experiments. Additionally, we explored the application of hydrogel materials in the scenario of postoperative bleeding, which can effectively prevent unnecessary adhesion through rapid film formation and the anti-protein property of sulfobetaine. We believe that this multifunctional hemostatic hydrogel has the potential to serve as a prehospital emergency treatment of incompressible bleeding and benefit to the postoperative recovery of patients.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101431"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain-targeting drug delivery systems: The state of the art in treatment of glioblastoma","authors":"Bo Sun ,&nbsp;Rong Li ,&nbsp;Ning Ji ,&nbsp;Han Liu ,&nbsp;Hongxiang Wang ,&nbsp;Chao Chen ,&nbsp;Long Bai ,&nbsp;Jiacan Su ,&nbsp;Juxiang Chen","doi":"10.1016/j.mtbio.2025.101443","DOIUrl":"10.1016/j.mtbio.2025.101443","url":null,"abstract":"<div><div>Glioblastoma (GBM) is the most prevalent primary malignant brain tumor, characterized by a high mortality rate and a poor prognosis. The blood-brain barrier (BBB) and the blood-tumor barrier (BTB) present significant obstacles to the efficacy of tumor-targeted pharmacotherapy, thereby impeding the therapeutic potential of numerous candidate drugs. Targeting delivery of adequate doses of drug across the BBB to treat GBM has become a prominent research area in recent years. This emphasis has driven the exploration and evaluation of diverse technologies for GBM pharmacotherapy, with some already undergoing clinical trials. This review provides a thorough overview of recent advancements and challenges in targeted drug delivery for GBM treatment. It specifically emphasizes systemic drug administration strategies to assess their potential and limitations in GBM treatment. Furthermore, this review highlights promising future research directions in the development of intelligent drug delivery systems aimed at overcoming current challenges and enhancing therapeutic efficacy against GBM. These advancements not only support foundational research on targeted drug delivery systems for GBM but also offer methodological approaches for future clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101443"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization","authors":"Junwei Su ,&nbsp;Yifan Wu ,&nbsp;Zheng Wang ,&nbsp;Dong Zhang ,&nbsp;Xianquan Yang ,&nbsp;Yong Zhao ,&nbsp;Aixi Yu","doi":"10.1016/j.mtbio.2025.101444","DOIUrl":"10.1016/j.mtbio.2025.101444","url":null,"abstract":"<div><div>Osteomyelitis has gradually become a catastrophic complication in orthopedic surgery due to the formation of bacterial biofilms on the implant surface and surrounding tissue. The therapeutic challenges of antibiotic resistance and poor postoperative osseointegration provide inspiration for the development of bioactive implants. We have strategically designed bioceramic scaffolds modified with <em>Lactobacillus reuteri</em> (LR) and bacteriophages (phages) to achieve both antibacterial and osteogenic effects. Leveraging the tendency of bacteria to adhere to the surface of implants, bioceramics have been modified with LR biofilm to promote bone repair. The LR biofilm, sterilized by pasteurization, prevents sepsis caused by live bacteria and is biocompatible with phages. Phages, being natural enemies of bacteria, not only effectively kill bacteria and inhibit biofilm formation but also readily adsorb onto the surface of bioceramics. Hence, this scaffold, loaded with a phage cocktail, lysates specific bacterial populations, namely <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Staphylococcus aureus</em> (<em>S. aureus</em>). More importantly, the inactivated LR biofilm stimulates macrophages RAW264.7 to polarize towards an anti-inflammatory M2 phenotype, creating an immune microenvironment favorable for inducing osteogenic differentiation of rat mesenchymal stem cells <em>in vitro</em>. In a rat model of infectious cranial defects, the scaffold not only effectively eliminated <em>S. aureus</em> and alleviated associated inflammation but also mediated macrophage-mediated immunoregulation, thus resulting in effective osteogenesis. Collectively, these multifunctional modified scaffolds offer an integrated approach to both bacterium elimination and bone repair, presenting a new strategy for bioactive implants in the clinical management of osteomyelitis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101444"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal-enhanced silver nanocluster bioactive glass hydrogels for synergistic antimicrobial and promote wound healing","authors":"Fuqiang Song ,&nbsp;Anqi Ye ,&nbsp;Linyuan Jiang ,&nbsp;Yang Lu ,&nbsp;Yanzhen Feng ,&nbsp;Rong Huang ,&nbsp;Siting Du ,&nbsp;Xiaoyu Dong ,&nbsp;Ting Huang ,&nbsp;Ping Li ,&nbsp;Liangliang Yang ,&nbsp;Jinjing Zhang ,&nbsp;Mengjia Xu ,&nbsp;Li Cheng ,&nbsp;Jian Xiao","doi":"10.1016/j.mtbio.2024.101439","DOIUrl":"10.1016/j.mtbio.2024.101439","url":null,"abstract":"<div><div>Antibacterial hydrogels are promising for combating infections and promoting wound healing. Nevertheless, excessive antibiotics induce resistance, and high metal ion levels cause cytotoxicity, complicating healing. Here, we introduce a hydrogel incorporating polydopamine-coated bioactive glass (BGs@PDA) on reduced graphene oxide (rGO) with photothermal therapy (PTT) and silver nanoclusters (AgNCs) for synergistic antibacterial treatment. This design enables rapid bacterial eradication and controlled release. Near-infrared-assisted heating provides noninvasive, targeted hyperthermia, killing bacteria quickly. Post-PTT addition of low-dose AgNCs reduces toxicity while enhancing antimicrobial efficacy and biocompatibility. BGs@PDA-loaded rGO prevents sedimentation, improves photothermal conversion and conductivity, and stabilizes the hydrogel structure. Constructed from chitosan and hydroxyethyl cellulose, the hydrogel is cross-linked by PDA and rGO, enhancing mechanical strength, adhesion, self-healing, free radical scavenging, and continuous wound exudate absorption. PDA encapsulation facilitates BGs degradation, improving the wound microenvironment. In vivo studies confirm accelerated healing and potent synergistic antibacterial effects, indicating its potential as a low-dose, antibiotic-free alternative for clinical wound infection management.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101439"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration","authors":"You Wu ,&nbsp;Xiaokun Yue ,&nbsp;Ying Zhang ,&nbsp;Ning Yu ,&nbsp;Chengyan Ge ,&nbsp;Rui Liu ,&nbsp;Zhongying Duan ,&nbsp;Lilong Gao ,&nbsp;Xinlong Zang ,&nbsp;Xin Sun ,&nbsp;Deteng Zhang","doi":"10.1016/j.mtbio.2024.101436","DOIUrl":"10.1016/j.mtbio.2024.101436","url":null,"abstract":"<div><div>Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal scaffolds must provide physical guidance to recruit cells and immune regulation to improve the regenerative microenvironment. This study presents a novel scaffold composed of dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with a dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue nanoparticles (PB@PLS). The microgrooves on the surface of the PLCL film were imprinted using a micropatterned polydimethylsiloxane (PDMS) template. Following aminolysis, the PLCL film was covalently grafted with the EM-7 peptide via glutaraldehyde. Functional group analysis, surface morphology and hydrophilicity were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and an optical contact angle measuring instrument, respectively. Bone regeneration-related cells (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann cells, and endothelial cells) cultured on PLCL films tended to align along the stripes and migrate from the periphery toward the center region <em>in vitro</em>. Subsequently, the PLCL film was encapsulated in an immune-regulating hydrogel synthesized from thiol-modified gelatin and Cu²⁺ in the presence of PB@PLS nanoparticles, which demonstrated excellent antioxidant properties. This scaffold significantly accelerated critical-sized bone regeneration, as evidenced by an increase in the volume of newly formed bone and histological images <em>in vivo</em>. This innovative approach holds substantial promise for clinical applications in bone regeneration and broader tissue repair.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"30 ","pages":"Article 101436"},"PeriodicalIF":8.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}