Materials Today Bio最新文献_第8页

Materials-based hair follicle engineering: Basic components and recent advances 基于材料的毛囊工程：基本成分和最新进展

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-18 DOI: 10.1016/j.mtbio.2024.101303

Yudie Lv , Weili Yang , Perumal Ramesh Kannan , Han Zhang , Rui Zhang , Ruibo Zhao , Xiangdong Kong

{"title":"Materials-based hair follicle engineering: Basic components and recent advances","authors":"Yudie Lv , Weili Yang , Perumal Ramesh Kannan , Han Zhang , Rui Zhang , Ruibo Zhao , Xiangdong Kong","doi":"10.1016/j.mtbio.2024.101303","DOIUrl":"10.1016/j.mtbio.2024.101303","url":null,"abstract":"<div><div>The hair follicle (HF) is a significant skin appendage whose primary function is to produce the hair shaft. HFs are a non-renewable resource; skin damage or follicle closure may lead to permanent hair loss. Advances in biomaterials and biomedical engineering enable the feasibility of manipulating the HF-associated cell function for follicle reconstruction via rational design. The regeneration of bioengineered HF addresses the issue of limited resources and contributes to advancements in research and applications in hair loss treatment, HF development, and drug screening. Based on these requirements, this review summarizes the basic and recent advances in hair follicle regulation, including four components: acquisition of stem cells, signaling pathways, materials, and engineering methods. Recent studies have focused on efficiently combining these components and reproducing functionality, which would boost fabrication in HF rebuilding ex vivo, thereby eliminating the obstacles of transplantation into animals to promote mature development.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101303"},"PeriodicalIF":8.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response 硅胶植入体的硬度和表面拓扑结构竞争性地介导了巨噬细胞的炎症反应和异物反应

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-18 DOI: 10.1016/j.mtbio.2024.101304

Sicen He , Qingrong Zhang , Jiezhi Jia , Wei Xia , Shengnan Chen , Fanyi Min , Yanlin Song , Yunlong Yu , Jiangfeng Li , Zheng Li , Gaoxing Luo

{"title":"Stiffness and surface topology of silicone implants competitively mediate inflammatory responses of macrophages and foreign body response","authors":"Sicen He , Qingrong Zhang , Jiezhi Jia , Wei Xia , Shengnan Chen , Fanyi Min , Yanlin Song , Yunlong Yu , Jiangfeng Li , Zheng Li , Gaoxing Luo","doi":"10.1016/j.mtbio.2024.101304","DOIUrl":"10.1016/j.mtbio.2024.101304","url":null,"abstract":"<div><div>Adverse inflammatory responses, dominated by macrophages, that are induced by physical cues of silicone implants can heavily damage the life quality of patients via causing fibrosis and device failure. As stiffness and surface topology affect macrophages at the same time, the competition or partnership among physical cues against the regulation of macrophages is still ambiguous. Herein, a series of PDMS implants with different stiffness at ∼ MPa and surface topology at tens of micrometers were fabricated to investigate the relationship, the regulation rule, and the underlying mechanism of the two physical cues against the inflammatory responses of M1 macrophages. There is a competitive rule: surface topology could suppress the inflammatory responses of M1 macrophages in the soft group but did not have the same effect in the stiff group. Without surface topology, lower stiffness unexpectedly evoked stronger inflammatory responses of M1 macrophages. Implanting experiments also proved that the competitive state against mediating <em>in vivo</em> immune responses and the unexpected inflammatory responses. The reason is that stiffness could strongly up-regulate focal adhesion and activate the MAPK/NF-κB signaling axis to evoke inflammatory responses, which could shield the effect of surface topology. Therefore, for patient healthcare, it is crucial to prioritize stiffness while not surface topology at MPa levels to minimize adverse reactions.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101304"},"PeriodicalIF":8.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuproptosis-based layer-by-layer silk fibroin nanoplatform-loaded PD-L1 siRNA combining photothermal and chemodynamic therapy against metastatic breast cancer 基于逐层丝纤维素纳米平台的 PD-L1 siRNA 光热疗法和化学动力疗法联合治疗转移性乳腺癌

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-16 DOI: 10.1016/j.mtbio.2024.101298

Zheng Li , Lan Cheng , Xiang Xu , Rui Jia , Siyu Zhu , Qian Zhang , Guotao Cheng , Baiqing Wu , Zulan Liu , Xiaoling Tong , Bo Xiao , Fangyin Dai

{"title":"Cuproptosis-based layer-by-layer silk fibroin nanoplatform-loaded PD-L1 siRNA combining photothermal and chemodynamic therapy against metastatic breast cancer","authors":"Zheng Li , Lan Cheng , Xiang Xu , Rui Jia , Siyu Zhu , Qian Zhang , Guotao Cheng , Baiqing Wu , Zulan Liu , Xiaoling Tong , Bo Xiao , Fangyin Dai","doi":"10.1016/j.mtbio.2024.101298","DOIUrl":"10.1016/j.mtbio.2024.101298","url":null,"abstract":"<div><div>Cuproptosis is a newly identified form of copper-dependent cell death that differs from other known pathways. This discovery provides a new way to explore copper-based nanomaterial applications in cancer therapy. This study used a layer-by-layer self-assembling method to load Cu<sub>2-x</sub>S nanoparticle (NP) cores with the siRNA of the <em>PD-L1</em> immune escape-related gene and wrap a silk fibroin (SF) shell to form a multifunctional copper-based SF nanoplatform, denoted as CuS-PEI-siRNA-SFNs. CuS-PEI-siRNA-SFNs induced cuproptosis and exerted an antitumor effect via multiple mechanisms, including photothermal therapy (PTT), chemodynamic therapy (CDT), and immune activation. The presence of significant dihydrolipoamide <em>S</em>-acetyltransferase (DLAT) oligomers in 4T1 cells treated with CuS-PEI-siRNA-SFNs indicated the triggering of cuproptosis. Furthermore, <em>in vivo</em> experimental results showed that CuS-PEI-siRNA-SFNs efficiently accumulated in the tumor tissues of 4T1 tumor-bearing mice inhibited primary tumor and lung metastasis, and displayed excellent biosafety and antitumor activity. This study demonstrated that the synergistic effect of cuproptosis, PTT, CDT, and immune activation showed promise for treating metastatic breast cancer.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101298"},"PeriodicalIF":8.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway 铜绿微球通过 Wnt/β-catenin 通路抑制骨关节炎中的杯突症和氧化应激

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-16 DOI: 10.1016/j.mtbio.2024.101300

Bo Li , Tongmeng Jiang , Juan Wang , Hongping Ge , Yaqi Zhang , Tong Li , Chen Wang , Weiguo Wang

{"title":"Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway","authors":"Bo Li , Tongmeng Jiang , Juan Wang , Hongping Ge , Yaqi Zhang , Tong Li , Chen Wang , Weiguo Wang","doi":"10.1016/j.mtbio.2024.101300","DOIUrl":"10.1016/j.mtbio.2024.101300","url":null,"abstract":"<div><div>This study aims to evaluate the therapeutic potential of cuprorivaite microspheres for osteoarthritis (OA), in particular, potential molecular mechanisms were investigated. The microspheres were developed from Ca(NO<sub>3</sub>)<sub>2</sub>•4H<sub>2</sub>O, Cu(NO<sub>3</sub>)<sub>2</sub>•3H<sub>2</sub>O, and silica gel, and further therapeutic effects were tested <em>in vitro</em> on mouse primary chondrocytes treated with interleukin-1β (IL-1β) to mimic OA, and <em>in vivo</em> on OA mice induced via anterior cruciate ligament transection (ACLT) surgery. The microspheres were shown to mitigate IL-1β-induced apoptotic, inflammatory, oxidative stress and cuproptosis markers while enhancing cell viability and extracellular matrix (ECM) components in chondrocytes. Moreover, the microspheres ameliorated histopathological damage, reduced inflammatory, oxidative stress and cuproptosis markers, and enhanced ECM biomarker levels in OA mice, implicating their role in suppressing cuproptosis and oxidative stress. The aforementioned effects of the cuprorivaite microspheres were demonstrated by using SKL2001, an agonist of the Wnt/β-catenin pathway. The results suggest cuprorivaite microspheres as a promising intervention for OA and cartilage regeneration, highlighting their therapeutic effects on cellular and molecular levels.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101300"},"PeriodicalIF":8.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intelligent nanocatalyst mediated lysosomal ablation pathway to coordinate the amplification of tumor treatment 智能纳米催化剂介导的溶酶体消融途径协调肿瘤治疗的放大效应

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-16 DOI: 10.1016/j.mtbio.2024.101299

Mingliang Pei , Xin Guan , De Zhao , Fan Yang , Yun Dong , Manxiu Huai , Wensong Ge , Xiaodong Hou , Wenfeng Chu , Kai Wang , Jie Chen , Huixiong Xu

{"title":"Intelligent nanocatalyst mediated lysosomal ablation pathway to coordinate the amplification of tumor treatment","authors":"Mingliang Pei , Xin Guan , De Zhao , Fan Yang , Yun Dong , Manxiu Huai , Wensong Ge , Xiaodong Hou , Wenfeng Chu , Kai Wang , Jie Chen , Huixiong Xu","doi":"10.1016/j.mtbio.2024.101299","DOIUrl":"10.1016/j.mtbio.2024.101299","url":null,"abstract":"<div><div>The production of reactive oxygen species (ROS) is susceptible to external excitation or insufficient supply of related participants (<em>e.g.</em>, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and sensitizer), liming ROS-driven tumor treatment. Additionally, the lysosomal retention effect severely hinders the utilization of ROS-based nanosystems and severely restricted the therapeutic effect of tumors. Therefore, first reported herein an intelligent nanocatalyst, TCPP-Cu@MnO<sub>x</sub> ((Mn<sup>II</sup>)<sub>1</sub>(Mn<sup>III</sup>)<sub>2.1</sub>(Mn<sup>IV</sup>)<sub>2.6</sub>O<sub>9.35</sub>), and proposed a programmed ROS amplification strategy to treat tumors. Initially, the acidity-unlocked nanocatalyst was voluntarily triggered to generate abundant singlet oxygen (<sup>1</sup>O<sub>2</sub>) to mediate acid lysosomal ablation to assist nanocatalyst escape and partially induce lysosomal death, a stage known as lysosome-driven therapy. More unexpectedly, the high-yielding production of <sup>1</sup>O<sub>2</sub> in acid condition (pH 5.0) was showed compared to neutral media (pH 7.4), with a difference of about 204 times between the two. Subsequently, the escaping nanocatalyst further activated H<sub>2</sub>O<sub>2</sub>-mediated <sup>1</sup>O<sub>2</sub> and hydroxyl radical (•OH) generation and glutathione (GSH) consumption for further accentuation tumor therapy efficiency, which is based on the Fenton-like reaction and Russell reaction mechanisms. Therefore, in this system, a program-activatable TCPP-Cu@MnO<sub>x</sub> nanocatalyst, was proposed to efficiently destruct organelle-lysosome <em>via</em> <sup>1</sup>O<sub>2</sub> inducing, and stimulated H<sub>2</sub>O<sub>2</sub> conversion into highly toxic <sup>1</sup>O<sub>2</sub> and •OH in cytoplasm, constituting an attractive method to overcome limitations of current ROS treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101299"},"PeriodicalIF":8.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable hydrogels for Fenton-like Mn2+/Fe2+ delivery with enhanced chemodynamic therapy prevent osteosarcoma recurrence and promote wound healing after excision surgery 用于 Fenton 类 Mn2+/Fe2+ 递送的可注射水凝胶可增强化学动力疗法，防止骨肉瘤复发并促进切除手术后的伤口愈合

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-11 DOI: 10.1016/j.mtbio.2024.101297

Lutong Wang , Haoyu Guo , Weiyue Zhang , Xingyin Li , Ziliang Su , Xin Huang

{"title":"Injectable hydrogels for Fenton-like Mn2+/Fe2+ delivery with enhanced chemodynamic therapy prevent osteosarcoma recurrence and promote wound healing after excision surgery","authors":"Lutong Wang , Haoyu Guo , Weiyue Zhang , Xingyin Li , Ziliang Su , Xin Huang","doi":"10.1016/j.mtbio.2024.101297","DOIUrl":"10.1016/j.mtbio.2024.101297","url":null,"abstract":"<div><div>Local recurrence of osteosarcoma and wound healing after excision surgery are major challenges in clinical research. The present anti-tumor treatments could inhibit normal tissues, resulting in difficulties in surgical wound healing. In this study, we constructed an injectable hydrogel as a platform to co-deliver MnO<sub>2</sub> nanoparticles and ferrocene Fc, termed as (MnO<sub>2</sub>/Fc)@PLGA for osteosarcoma treatment and wound healing after excision. By simple local injection, the hydrogel could form a protective barrier on the surgical wound after osteosarcoma excision, which could promote wound healing and steady release of MnO<sub>2</sub>/Fc nanoparticles. The released MnO<sub>2</sub>/Fc might undergo the Fenton reaction through Mn<sup>2+</sup>/Fe<sup>2+</sup> to inhibit osteosarcoma cells with chemodynamic therapy (CDT). Furthermore, MnO<sub>2</sub> could catalyze endogenous H<sub>2</sub>O<sub>2</sub> to produce O<sub>2</sub>, which eliminates the adverse effects of H<sub>2</sub>O<sub>2</sub> and remodels the hypoxic state in the local lesions. The increased O<sub>2</sub> facilitated surgical wound healing and anti-tumor effects by regulating the hypoxia inducible factor-1 functions. In conclusion, (MnO<sub>2</sub>/Fc)@PLGA hydrogel could effectively prevent local recurrence of osteosarcoma and promote wound healing after excision surgery, thereby providing a novel strategy for tumor treatment and tissue repair.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101297"},"PeriodicalIF":8.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative hydrogel-based therapies for ischemia-reperfusion injury： bridging the gap between pathophysiology and treatment 基于水凝胶的缺血再灌注损伤创新疗法：弥合病理生理学与治疗之间的差距

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-10 DOI: 10.1016/j.mtbio.2024.101295

Weibo Wang , Supeng Tai , Junyue Tao , Lexing Yang , Xi Cheng , Jun Zhou

{"title":"Innovative hydrogel-based therapies for ischemia-reperfusion injury： bridging the gap between pathophysiology and treatment","authors":"Weibo Wang , Supeng Tai , Junyue Tao , Lexing Yang , Xi Cheng , Jun Zhou","doi":"10.1016/j.mtbio.2024.101295","DOIUrl":"10.1016/j.mtbio.2024.101295","url":null,"abstract":"<div><div>Ischemia-reperfusion injury (IRI) commonly occurs in clinical settings, particularly in medical practices such as organ transplantation, cardiopulmonary resuscitation, and recovery from acute trauma, posing substantial challenges in clinical therapies. Current systemic therapies for IRI are limited by poor drug targeting, short efficacy, and significant side effects. Owing to their exceptional biocompatibility, biodegradability, excellent mechanical properties, targeting capabilities, controlled release potential, and properties mimicking the extracellular matrix (ECM), hydrogels not only serve as superior platforms for therapeutic substance delivery and retention, but also facilitate bioenvironment cultivation and cell recruitment, demonstrating significant potential in IRI treatment. This review explores the pathological processes of IRI and discusses the roles and therapeutic outcomes of various hydrogel systems. By categorizing hydrogel systems into depots delivering therapeutic agents, scaffolds encapsulating mesenchymal stem cells (MSCs), and ECM-mimicking hydrogels, this article emphasizes the selection of polymers and therapeutic substances, and details special crosslinking mechanisms and physicochemical properties, as well as summarizes the application of hydrogel systems for IRI treatment. Furthermore, it evaluates the limitations of current hydrogel treatments and suggests directions for future clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101295"},"PeriodicalIF":8.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-scavenging ultrasonicated graphene oxide/alginate microgels for mesenchymal stem cell delivery and hindlimb ischemia treatment 清除 ROS 的超声氧化石墨烯/精氨酸微凝胶用于间充质干细胞输送和后肢缺血治疗

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-10 DOI: 10.1016/j.mtbio.2024.101289

Seungjun Lee , Goeun Choe , Jongdarm Yi , Junghyun Kim , Sun Hong Lee , Jin Jeon , Hee Seok Yang , Jae Young Lee

{"title":"ROS-scavenging ultrasonicated graphene oxide/alginate microgels for mesenchymal stem cell delivery and hindlimb ischemia treatment","authors":"Seungjun Lee , Goeun Choe , Jongdarm Yi , Junghyun Kim , Sun Hong Lee , Jin Jeon , Hee Seok Yang , Jae Young Lee","doi":"10.1016/j.mtbio.2024.101289","DOIUrl":"10.1016/j.mtbio.2024.101289","url":null,"abstract":"<div><div>Mesenchymal stem cell (MSC) transplantation is widely recognized as a promising treatment for peripheral artery diseases because of their unique ability to secrete multiple growth factors and immunomodulatory cytokines. However, direct administration of MSCs frequently results in insufficient therapeutic efficacy due to low viability and poor retention at the implantation site. The delivery of MSCs in microsized hydrogels allows for simple injection, improved retention, and enhanced cell protection. However, the high oxidative stress present in ischemic tissues significantly impairs the viability and therapeutic activity of transplanted MSCs. This study aimed to develop a simple and effective method for fabricating reactive oxygen species (ROS)-scavenging microgels to enhance the MSC efficacy for ischemic hindlimb treatment. Specifically, tip-sonicated graphene oxide (GO)/alginate (sGO/alginate) microgels exhibited significantly increased antioxidizing activity against various ROS compared with pristine GO/alginate microgels. MSCs encapsulated in sGO/alginate microgels (MSC/sGO/alginate) demonstrated higher viability than those encapsulated in alginate or GO/alginate microgels under various oxidative stress conditions. Furthermore, human umbilical vein endothelial cells co-cultured with MSCs encapsulated in sGO/alginate microgels formed more tubes under both normal and H<sub>2</sub>O<sub>2</sub>-treated conditions, implying enhanced pro-angiogenic potential of the MSCs. In vivo experiments using hindlimb ischemia mouse models revealed significant improvements in blood perfusion, limb salvage, vascularization, and MSC survival in the MSC/sGO/alginate group compared with the other groups (MSC, MSC/alginate, and MSC/GO/alginate). The strategy developed in this study offers a straightforward and powerful method for treating various ROS-related diseases, including ischemia.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101289"},"PeriodicalIF":8.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic composite gelatin methacryloyl hydrogels for improving survival and osteogenesis of human adipose-derived stem cells in 3D microenvironment 仿生复合明胶甲基丙烯酰水凝胶用于提高人脂肪来源干细胞在三维微环境中的存活率和成骨率

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-09 DOI: 10.1016/j.mtbio.2024.101293

Eunhyung Kim , Jinkyu Lee , Se-Jeong Kim , Eun Mi Kim , Hayeon Byun , Seung Jae Huh , Eunjin Lee , Heungsoo Shin

{"title":"Biomimetic composite gelatin methacryloyl hydrogels for improving survival and osteogenesis of human adipose-derived stem cells in 3D microenvironment","authors":"Eunhyung Kim , Jinkyu Lee , Se-Jeong Kim , Eun Mi Kim , Hayeon Byun , Seung Jae Huh , Eunjin Lee , Heungsoo Shin","doi":"10.1016/j.mtbio.2024.101293","DOIUrl":"10.1016/j.mtbio.2024.101293","url":null,"abstract":"<div><div>Gelatin methacryloyl (GelMA) hydrogels are used for stem cell encapsulation in bone tissue engineering due to their fast and stable photo-crosslinking. However, cell viability and ability to induce osteogenesis are reduced by reactive oxygen species (ROS) produced during the crosslinking reaction. In this study, we developed biomimetic nanoparticles (TMNs) by combining tannic acid (TA) and simulated body fluid (SBF) minerals, and used them to synthesize GelMA-based composite hydrogels for addressing those limitations. The optimal concentrations of TA and SBF were investigated to create nanoparticles that can effectively scavenge ROS and induce osteogenesis. The incorporation of TMNs into composite hydrogels (G-TMN) significantly enhanced the survival and proliferation of encapsulated human adipose-derived stem cells (hADSCs) by providing resistance to oxidative conditions. In addition, the ions that were released, such as Ca<sup>2+</sup> and PO<sub>4</sub><sup>3−</sup>, stimulated stem cell differentiation into bone cells. The hADSCs encapsulated in G-TMN had 2.0 ± 0.8-fold greater viability and 1.3 ± 1.8 times greater calcium deposition than those encapsulated in the hydrogel without nanoparticles. Furthermore, the <em>in vivo</em> transplantation of G-TMN into a subcutaneous mouse model demonstrated the rapid degradation of the gel-network while retaining the osteoinductive particles and cells in the transplanted area. The increased cellular activity observed in our multifunctional composite hydrogel can serve as a foundation for novel and effective therapies for bone deformities.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101293"},"PeriodicalIF":8.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmable biomaterials for bone regeneration 用于骨再生的可编程生物材料

IF 8.7 1区医学

Materials Today Bio Pub Date : 2024-10-09 DOI: 10.1016/j.mtbio.2024.101296

Peiran Song , Dongyang Zhou , Fuxiao Wang , Guangfeng Li , Long Bai , Jiacan Su

{"title":"Programmable biomaterials for bone regeneration","authors":"Peiran Song , Dongyang Zhou , Fuxiao Wang , Guangfeng Li , Long Bai , Jiacan Su","doi":"10.1016/j.mtbio.2024.101296","DOIUrl":"10.1016/j.mtbio.2024.101296","url":null,"abstract":"<div><div>Programmable biomaterials are distinguished by their ability to adjust properties and functions on demand, in a periodic, reversible, or sequential manner. This contrasts with traditional biomaterials, which undergo irreversible, uncontrolled changes. This review synthesizes key advances in programmable biomaterials, examining their design principles, functionalities and applications in bone regeneration. It charts the transition from traditional to programmable biomaterials, emphasizing their enhanced precision, safety and control, which are critical from clinical and biosafety standpoints. We then classify programmable biomaterials into six types: dynamic nucleic acid-based biomaterials, electrically responsive biomaterials, bioactive scaffolds with programmable properties, nanomaterials for targeted bone regeneration, surface-engineered implants for sequential regeneration and stimuli-responsive release materials. Each category is analyzed for its structural properties and its impact on bone tissue engineering. Finally, the review further concludes by highlighting the challenges faced by programmable biomaterials and suggests integrating artificial intelligence and precision medicine to enhance their application in bone regeneration and other biomedical fields.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101296"},"PeriodicalIF":8.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0