Materials Today Bio最新文献

{"title":"A dual-component particulate dressing for simultaneous microenvironment modulation and tissue regeneration in infected diabetic wounds","authors":"Jianwei Li ,&nbsp;Mengkun Wang ,&nbsp;Xiangzhong Tan ,&nbsp;Yingxi Duanmiao ,&nbsp;Xiang Zheng ,&nbsp;Zhen Wang ,&nbsp;Fei Gao ,&nbsp;Yuqing Miao ,&nbsp;Huipeng Li ,&nbsp;Bo Liu ,&nbsp;Mingjun Li ,&nbsp;Tingbin Zhang ,&nbsp;Huan Zhou ,&nbsp;Jinfeng Xing ,&nbsp;Lei Yang","doi":"10.1016/j.mtbio.2025.102005","DOIUrl":"10.1016/j.mtbio.2025.102005","url":null,"abstract":"<div><div>The treatment of chronic diabetic wounds faces considerable challenges owing to complex environments in the wound bed, such as chronic inflammation, excessive reactive oxygen species (ROS), impaired extracellular matrix (ECM) and bacterial infection. Current strategies, including bandages, hydrogel dressings and medical devices, that focus solely on a few pathological features have limited success. Herein, a fast self-gelling polyacrylic acid (PAA) derivative/madecassoside (MA) particulate dressing with anti-inflammatory, antioxidative, collagen deposition-promoting and intrinsic antibacterial properties is developed to simultaneously regulate the wound microenvironment and promote tissue regeneration in infected diabetic wounds. The incorporation of N-[Tris(hydroxymethyl)methyl]acrylamides (THMA), a small molecule compound that has three hydroxy groups clustered together, into the PAA backbone confers the copolymer with self-gelation, robust wet tissue adhesion and a strong capacity to load MA via multiple hydrogen bonding. The developed dual-component particulate dressing effectively regulated macrophage polarization towards the anti-inflammatory phenotype, and displayed potent antibacterial activity against both Gram-positive <em>S. aureus</em> (99.2 %) and Gram-negative <em>E. coli</em> (90.8 %) at a dose of 8 mg mL⁻¹. Further, the dressing obviously accelerated the healing of full-thickness skin wounds compared with commercial fibrin glue in a <em>S. aureus</em>-infected diabetic mouse model. This multifunctional PAA-based wound dressing is potentially valuable for clinical applications towards diabetic foot ulcers, pressure ulcers and other conditions of acute or chronic wounds.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 102005"},"PeriodicalIF":8.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming cellular senescence of hepatic stellate cells to combat liver fibrosis by targeted nanodrugs","authors":"Bingyuan Fei ,&nbsp;Hui Wang ,&nbsp;Yu Ding ,&nbsp;Nannan Shao ,&nbsp;Panyue Wen ,&nbsp;Yanwei Cao ,&nbsp;Junjie Li ,&nbsp;Masaru Tanaka ,&nbsp;Zheng Wang ,&nbsp;Shuo Li","doi":"10.1016/j.mtbio.2025.101996","DOIUrl":"10.1016/j.mtbio.2025.101996","url":null,"abstract":"<div><div>Senescence of activated hepatic stellate cells (aHSCs) is thought to be a promising alternative for limiting hepatic fibrosis. However, uncontrollable accumulation and spread of senescence in neighboring hepatocytes lead to inflammation and steatosis, aggravating fibrosis and even promoting carcinogenesis. To harness senescence for fibrotic treatment, aHSCs-targeted poly (lactic-co-glycolic acid) (PLGA) nanoplatforms have been constructed to integrate senescent induction of aHSCs and senescent reprogramming. Owing to the CD44 aptamer modification, the nanoplatform specifically delivers senescent inducers and small interfering RNAs (siRNAs) that silence nuclear factor-kappa B (NF-κB) in aHSCs, thereby inducing senescence and simultaneously suppressing the production of senescence-associated secretory phenotypes (SASPs) in aHSCs. The senescence of aHSCs decreases their proliferation, and achieves permanent inactivation even upon repeated fibrotic stimulus. Meanwhile, the elimination of SASPs interrupts the vicious cycle of senescent aHSCs with surrounding hepatocytes to decrease senescent and inflammatory accumulation in liver tissues. In vitro and in vivo results confirmed the superior ability of the nanoplatform to inhibit liver fibrosis and control the spread of senescence. Our work provides a nanoplatform for specifically inducing senescence of aHSCs and reveals a promising senescence modulation strategy for the treatment of liver fibrosis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101996"},"PeriodicalIF":8.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-assembled triptolide prodrug nanovesicles loading with ginsenoside Rg3 for double-targeted therapy of pancreatic cancer","authors":"Jiaxing Wang ,&nbsp;Jingru Cui ,&nbsp;Yujie Chen ,&nbsp;Huijie Zhou ,&nbsp;Xiaofang Li ,&nbsp;Xiangxiang Wu ,&nbsp;Rongyi Zhou ,&nbsp;Huahui Zeng","doi":"10.1016/j.mtbio.2025.102004","DOIUrl":"10.1016/j.mtbio.2025.102004","url":null,"abstract":"<div><div>Triptolide (TP), derived from the herb Tripterygium wilfordii, has a highly potent antitumor effect, but its poor water solubility and high toxicity hinder its clinical use. Here, a novel triptolide prodrug (TP-PEG-SS) was synthesized by conjugating TP and stachydrine (SS) with polyethylene glycol (PEG), which endowed TP with high water solubility, the capability to target tumor mitochondria, significant antitumor efficacy and low toxicity. Subsequently, TP-PEG-SS was self-assembled with ginsenoside Rg3 and lecithin to form nanovesicles (NVs). The NVs exhibited double-targeted performance for actively targeting tumor mitochondria via electrostatic interaction and entering M2 macrophage via glucose transporter GLUT-1, thereby greatly inhibiting the tumor cell growth by triggering apoptosis of tumor and polarization of M1 macrophage. In Pan02 tumor-bearing mice, the NVs were selectively accumulated in the tumor regions and improved the immunosuppressive tumor microenvironment, thereby exerting a more potent synergistic antitumor effect of both Rg3 and TP, as well as less systemic toxicity than free TP. Consequently, the NVs is a promising antitumor nanovesicle with double-targeted capability, which may enhance the clinical applicability of TP.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 102004"},"PeriodicalIF":8.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic UV photo-protection of skin surface by structured epicuticular wax films","authors":"Anuja Das ,&nbsp;Luca Polacchi ,&nbsp;Jean-Yves Fouron ,&nbsp;Antoine Montaux-Lambert ,&nbsp;Laurent Billon ,&nbsp;Gustavo S. Luengo","doi":"10.1016/j.mtbio.2025.101991","DOIUrl":"10.1016/j.mtbio.2025.101991","url":null,"abstract":"<div><div>The realms of biomimicry encourage us to explore and replicate the remarkable functionalities found in the big variety of living organisms such as plants, birds, animals etc. Inspired by the ultraviolet (UV) reflective characteristics of self-assembled epicuticular wax of plant leaves, in this article, we present a biomimetic plant-inspired approach to pattern the surface of skin with wax coatings and enhance its UV resistance. Through a physico-chemical approach, we coat chemically homogenous (as well as heterogenous) chemical composition of waxes from its solution on quartz substrate. By controlling the self-assembly conditions, diverse surface morphologies are obtained with <em>Euphorbia cerifera</em> (commonly known as <em>Candelilla</em>, chemically heterogenous wax) and <em>Myristyl Palmitate</em> (present in <em>Phytolacca Acinosa</em>, chemically homogenous alkyl esters wax). Optical measurements show increased reflectance in visible spectra for <em>Candelilla</em> wax coatings exhibiting globules, plate-like crystalline structures at the surface which contributes to higher roughness parameters. With homogenous wax, maximum reflectance is obtained for dual scale morphology which includes self-assembled 3D plate-like structures at an optimum length-scale. Our experiments reveal that the combined effect of vertically and horizontally placed stacks of crystal plates at micron and sub-micron scales induces maximum scattering effect. This geometrical organisation effectively decreases transmission of incident radiations to the underlying surface leading to enhanced photo-protection. Further, to showcase the feasibility of such approach for potential cosmetic applications, we replicate best performing structures on a commonly used model skin surface for UV absorption evaluation (polymethyl methacrylate plates) and on real <em>ex-vivo Stratum Corneum,</em> the outermost layer of skin. For realistic substrates, scattering effect is additionally dependent on nature of intrinsic substrate patterns and roughness in which case the feature height (<strong>h_F</strong>) of 3D structures should be greater than substrate patterns to achieve maximum reflectance. This study highlights the formation of physical structuration with biomaterials and presents insights on scattering induced by such plant-based structures with potential for dermatological or cosmetic applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101991"},"PeriodicalIF":8.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered hybrid cell membrane nanosystems for treating cardiovascular diseases","authors":"He Lu ,&nbsp;Yaohui Jiang ,&nbsp;Rui Luo ,&nbsp;Dexing Zhou ,&nbsp;Feihu Zheng ,&nbsp;Liran Shi ,&nbsp;He Zhang ,&nbsp;Yong Wang ,&nbsp;Xiaodong Xu ,&nbsp;Renfang Zou ,&nbsp;Yujing Zhou ,&nbsp;Shuai Ren ,&nbsp;Xiaocheng Wang ,&nbsp;Haiqiang Sang","doi":"10.1016/j.mtbio.2025.101992","DOIUrl":"10.1016/j.mtbio.2025.101992","url":null,"abstract":"<div><div>Cardiovascular diseases (CVDs) continue to represent a major challenge to global health, highlighting the urgent need for innovative treatment approaches. As a growing interdisciplinary field, nanotechnology has demonstrated significant potential for clinical applications. Nanomedicine development primarily focuses on improving the disease diagnosis and treatment through leveraging the distinct characteristics of engineered nanoparticles (NPs) to detect disease markers or deliver therapeutics to specific targets. Specifically, cell membrane-coated NPs offer enhanced targeting, biostability, and immune evasion. A significant benefit associated with this technology lies in its capacity to retain the functional and intrinsic properties of the source cells. However, while each cell membrane possesses distinct characteristics, a single cell membrane may not always address complex functional requirements. By combining membranes from different cell types, it becomes possible to integrate diverse functionalities, resulting in a more comprehensive solution. In this review, we aim to explore recent advancements in the hybrid cell membrane-coated NPs (HM/NPs) and their potential applications for the treatment of CVDs. We highlight the mechanisms underlying HM/NPs and their utilization in the therapeutic management of CVDs. Additionally, we examine the potential for clinical translation and discuss the key challenges encountered in this process.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101992"},"PeriodicalIF":8.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wet-electrospun porous freeform scaffold enhances colonisation of cells","authors":"Haoyu Wang ,&nbsp;Xueying Xu ,&nbsp;Yifei Qin ,&nbsp;Hongyi Chen ,&nbsp;Yuexin Wang ,&nbsp;Joel Turner ,&nbsp;Jianping Zhang ,&nbsp;Maryam Tamaddom ,&nbsp;Feng-Lei Zhou ,&nbsp;Gareth Williams ,&nbsp;Chaozong Liu","doi":"10.1016/j.mtbio.2025.101997","DOIUrl":"10.1016/j.mtbio.2025.101997","url":null,"abstract":"<div><div>Osteoarthritis is a degenerative disease characterized by the progressive deterioration of articular cartilage. Electrospun scaffolds have shown promise in the regeneration of degraded areas due to their highly interconnected and extracellular matrix-mimicking structures. However, current electrospun scaffold-based therapies are limited by the constraints of 2D cell culture. In this study, a novel wet-electrospinning technique to generate polycaprolactone (PCL) porous 3D scaffolds was developed. The wet-electrospun yarns were collected via vortex, allowing for loosely interconnected yarns, thereby enhancing cell infiltration. Sodium hydroxide (NaOH) treatment was used to introduce carboxyl groups on PCL fibres, followed by gelatin conjugation via N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) crosslinking. Comparative analysis between conventional electrospun 2D dense and wet-electrospun 3D porous scaffolds revealed significant advantages in porosity, reaching up to 99.5 % in the 3D matrices. Subsequent in vitro evaluations demonstrated full-thickness cell infiltration in the 3D high-porosity scaffold after 7 days, as confirmed by SEM and confocal images. Further analysis on day 14 revealed the deposition of glycosaminoglycans (GAGs) and collagen. This research introduces a novel technique for fabricating high-porosity scaffolds that facilitate full-thickness 3D cell culture. These novel high-porosity, gelatin-conjugated scaffolds enhance cell colonisation and deposition. Overall, these high-porosity scaffolds overcome the limitations of conventional electrospinning, enabling 3D cell culture and offering new opportunities for cartilage regeneration and reconstruction.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101997"},"PeriodicalIF":8.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zwitterionic chitosan-modified amorphous ZnCuAl-LDH eye drops for efficient treatment of bacterial keratitis","authors":"Tingting Hu ,&nbsp;Dandan Chu ,&nbsp;Haohao Cui ,&nbsp;Shibo Wang ,&nbsp;Mengke Wang ,&nbsp;Ruizheng Liang ,&nbsp;Jingguo Li ,&nbsp;Zhanrong Li","doi":"10.1016/j.mtbio.2025.101998","DOIUrl":"10.1016/j.mtbio.2025.101998","url":null,"abstract":"<div><div>Bacterial keratitis caused by microbial invasion represents a significant cause of visual impairment characterized by corneal perforation, epithelial defect, intense pain, and conjunctival congestion. The widespread misuse of antibiotic eye drops in clinical treatment has accelerated the global rise of antibiotic-resistant infections, dimming the prospects for antibiotic therapy. In response, inorganic nanomaterial-based antibacterial eye drops have been developed for bacterial keratitis leveraging their structural controllability, composition diversity and ease of functionalization, However, they typically exert antibacterial effects with the assistance of laser, ultrasound, or X-ray irradiation that may cause irritation or damage to the human eye, or involve intricate structural modifications for antibacterial activation. Herein, we propose zwitterionic chitosan-modified defective amorphous ZnCuAl-layered double hydroxide nanosheets (ZC@a-LDH) as an antibacterial agent for bacterial keratitis treatment. ZC@a-LDH nanosheets demonstrate significantly enhanced antimicrobial efficacy compared with their defect-free counterparts, effectively suppressing bacterial proliferation at a minimum inhibitory concentration of 12 μg mL⁻¹ without relying on external stimuli. The enhanced antimicrobial performance may be attributed to the combined action of electrostatic interactions, antibacterial ions (Zn²⁺/Cu²⁺) and enhanced charge transport facilitated by structural defects. Notably, ZC@a-LDH antibacterial eye drops demonstrate effective intervention in bacterial keratitis in mice, outperforming the conventional tobramycin in therapeutic efficacy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101998"},"PeriodicalIF":8.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-delivery paclitaxel and IR783 as nanoparticles for potentiated chemo-photothermal-immunotherapy of triple-negative breast cancer","authors":"Yue Huang ,&nbsp;Kui Wang ,&nbsp;Mengjun Yu ,&nbsp;Qiang Zhou ,&nbsp;Jia Wang ,&nbsp;Shiwen Chen ,&nbsp;Jing Gong ,&nbsp;Min Yang ,&nbsp;Jingbin Huang ,&nbsp;Yu Zhao","doi":"10.1016/j.mtbio.2025.101993","DOIUrl":"10.1016/j.mtbio.2025.101993","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) is highly malignant and invasive, which is the major challenge of breast cancer treatment. Although chemo-immunotherapy is currently the most promising treatment option, its efficacy in treating TNBC is limited due to challenges such as low immune response rate and severe side effects. In this study, a nanocarrier PEI-GCP(Z)/mPEG with excellent water solubility and high drug-loading capacity was designed, and it was used to load the paclitaxel (PTX) and photosensitizer IR783 to prepare nanoparticles PEI-GCP(Z)/mPEG@PTX@IR783 (PPI). The PPI demonstrated high loading efficiency for PTX and IR783, enabling the chemotherapy effect of PTX and the photothermal therapy under 808 nm laser irradiation, that combined chemotherapy and photothermal therapy. Furthermore, PPI could induce immunogenic cell death (ICD), promote dendritic cells (DCs) maturation, and increase the infiltration of cytotoxic T lymphocytes (CTLs) in the tumor site, thereby enhancing anti-tumor immunity. PPI also increased the proportion of effector memory T cells (Tem), thereby supporting long-term anti-tumor immunity. Both <em>in vitro</em> and <em>in vivo</em> studies demonstrated that PPI effectively targets tumor cells and significantly inhibits tumor growth through the combined effects of chemo-photothermal-immunotherapy. Therefore, PPI is a promising nanoparticle with multi-modal therapeutic effects, offering substantial potential for the treatment of TNBC.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101993"},"PeriodicalIF":8.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled releasing melatonin nanoparticles promote restoration of nucleus pulposus cell homeostasis for alleviating intervertebral disc degeneration","authors":"Cheng Yang ,&nbsp;Shaoshuai Song ,&nbsp;Xianhao Zhou ,&nbsp;Dejian Li ,&nbsp;Chenyang Xu ,&nbsp;Chaohong Yu ,&nbsp;Guantong Sun ,&nbsp;Kaili Lin ,&nbsp;Youzhuan Xie","doi":"10.1016/j.mtbio.2025.101994","DOIUrl":"10.1016/j.mtbio.2025.101994","url":null,"abstract":"<div><div>The intervertebral administration of anti-inflammatory drugs is a promising local delivery approach to the intervertebral disc integrity restoration for treatment of intervertebral disc degeneration (IVDD), whereas the fast drug clearance in an intervertebral space is always a troublesome issue for the local drug delivery. In this study, we have developed a zeolitic imidazolate framework 8 (ZIF-8) nanoplatform with pH-sensitive property to load melatonin molecules (MT), a hormone with anti-inflammatory and anti-oxidative effects. Thus-synthesized MT@ZIF-8 nanoparticles not only showed good cytocompatibility and pH-dependent drug releasing behavior, but also effectively decreased the intracellular reactive oxygen species level and inhibited the pro-inflammatory cytokine expression for <em>in vitro</em> nucleus pulposus cell (NPC) culture under lipopolysaccharide (LPS) stimulus. Additionally, it was found that NF-κB and MAPK signal pathways are significantly suppressed by the MT@ZIF-8 nanoparticles for the LPS-treated NPCs, revealing the underlying mechanism of restoring extracellular matrix synthesis of the LPS-treated NPCs by the MT@ZIF-8 nanoparticles. Last but not least, the recovery of intervertebral disc components and integrity were observed on a rat caudal IVDD model after injection of the MT@ZIF-8 nanoparticles into the intervertebral space. In brief, this work offered a promising local delivery strategy and a controlled releasing nanoplatform for the applications of melatonin in the IVDD therapy, which we think may have beneficial effects for future clinical treatment of low back pain caused by the IVDD.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101994"},"PeriodicalIF":8.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoencapsulation platform for oral delivery of peptides: In vitro stabilization of AvPAL and formulation of a gastrointestinal-resistant luciferase","authors":"Daniel Abella-López,&nbsp;Adrián López-Teijeiro,&nbsp;Tomás Pose-Boirazian ,&nbsp;Natalia Barreiro-Piñeiro,&nbsp;José M. Martínez-Costas","doi":"10.1016/j.mtbio.2025.101987","DOIUrl":"10.1016/j.mtbio.2025.101987","url":null,"abstract":"<div><div>Phenylketonuria (PKU) is a genetic metabolic disorder caused by an enzyme deficiency that leads to the accumulation of phenylalanine, which can cause neurotoxicity and several other problems. A potential alternative to the universal standard treatment based on a lifelong protein-restricted diet, is the development of oral replacement therapies using phenylalanine ammonia lyase from <em>Anabaena variabilis</em> (AvPAL). However, oral administration of polypeptides presents a major challenge due to gastrointestinal (GI) instability. To address this issue, the use of the IC-Tagging system as an advanced one step, <em>in cellulo</em> nanosphere (NS)-encapsulation strategy for protein stabilization and oral delivery is proposed. A highly active version of AvPAL was produced to which nanoencapsulation provides formidable thermostability, resistance to acidic pH, long-term storage stability and protection against proteolytic degradation. This latter characteristic, essential for oral delivery of polypeptides, is further enhanced by coating with chitosan the NS-encapsulated enzyme. Thus, a similarly nanoencapsulated and chitosan-coated luciferase displays sustained enzymatic activity through the entire GI transit when administered orally in mice, indicating the high protective capability of the system while maintaining the availability of the enzyme. Overall, these results highlight the potential and versatility for peptide-based oral delivery applications of this innovative methodology.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"33 ","pages":"Article 101987"},"PeriodicalIF":8.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}