Materials Today Bio最新文献

{"title":"Nanomaterials at the forefront of antimicrobial therapy by photodynamic and photothermal strategies.","authors":"Ling Mei, Yifan Zhang, Kaixi Wang, Sijing Chen, Tao Song","doi":"10.1016/j.mtbio.2024.101354","DOIUrl":"10.1016/j.mtbio.2024.101354","url":null,"abstract":"<p><p>In the face of the increasing resistance of microorganisms to traditional antibiotics, the development of innovative treatment methods is becoming increasingly urgent. Nanophototherapy technology can precisely target the infected area and achieve synergistic antibacterial effects in multiple modes. This phototherapy method has shown significant efficacy in treating diseases caused by drug-resistant bacteria, especially in the elimination of biofilms, where it has demonstrated strong dissolution capabilities. PTT utilizes photothermal agents to convert near-infrared light into heat, effectively killing bacteria and promoting tissue regeneration. Similarly, PDT utilizes photosensitizers, which produce reactive oxygen species (ROS) when activated by light, destroying the structure and function of bacterial cells. This review summarizes photothermal agents and photosensitizers used for antibacterial purposes. In conducting our literature review, we employed a systematic approach to ensure a comprehensive and representative selection of studies. Additionally, this article explores the potential of phototherapy in regulating wound microenvironments, promoting wound healing, and activating the immune system. Nanophototherapeutic materials show great potential for application in antibacterial treatment and are expected to provide innovative solutions for drug-resistant bacterial infections that traditional antibiotics are struggling to address.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101354"},"PeriodicalIF":8.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An advanced chitosan based sponges dressing system with antioxidative, immunoregulation, angiogenesis and neurogenesis for promoting diabetic wound healing","authors":"Xianmou Fan ,&nbsp;Zhihong Su ,&nbsp;Wanjun Zhang ,&nbsp;Haili Huang ,&nbsp;Chengzhang He ,&nbsp;Zeyong Wu ,&nbsp;Peihua Zhang","doi":"10.1016/j.mtbio.2024.101361","DOIUrl":"10.1016/j.mtbio.2024.101361","url":null,"abstract":"<div><div>Promoting wound nerve regeneration and synchronously initiating angiogenesis are critical factors in the healing process of diabetic wounds. However, existing research on diabetic wounds mainly focuses on angiogenesis, bacterial infection and reactive oxygen species, often failing to coordinate neurogenesis and angiogenesis. To coordinate the symbiosis of nerves and blood vessels in the diabetic wounds, we successfully designed a multifunctional chitosan (CS)-based sponges by regulating the structure of CS specifically for diabetic wound healing. This sponge, which facilitates effective exudate transfer and modulates the wound microenvironment, was constructed using hydroxybutyl CS grafted with thioctic acid (TA), named as HCT sponge. When applied in a humid environment, the hydrophobic side chains of the HCT sponge interact with self-assembled hydrophobic domains, forming gel-sponge composite. Experimental results showed that the adhesion strength of the HCT sponge to wet porcine skin was 70.3 kPa. Additionally, the sponge exhibited favorable degradability, cytocompatibility and antioxidant properties. As it is shown in the experiments in vitro, sponge can not only promote cell proliferation, migration, and blood vessel formation, but also promote M2 macrophage polarization. Moreover, the rat liver and femoral artery injury model validated that the HCT sponge can effectively treat heavy bleeding from wounds efficacy through quickly sealing wounds and the formation of multiple hemostatic dams. In vivo studies indicated that the HCT sponge significantly accelerated the diabetic wound healing process compared to the recombinant bovine basic fibroblast growth factor gel, achieving a better recovery from the HCT sponge after 15 days. Pathological results show that the designed novel sponge holds considerable promise for treating diabetic wound, allowing regenerative neurogenesis and angiogenesis at the wound site, which provides a significant potential for further improving clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101361"},"PeriodicalIF":8.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-Hydroxytryptophan artificial synaptic vesicles across the blood-brain barrier for the rapid-acting treatment of depressive disorder","authors":"Peng Chang ,&nbsp;Jingwen Ma ,&nbsp;Ke Li ,&nbsp;Wei Wang ,&nbsp;Dan Chen ,&nbsp;Zhe Liu ,&nbsp;Wenhua Zhan ,&nbsp;Yun Zeng ,&nbsp;Yonghua Zhan","doi":"10.1016/j.mtbio.2024.101357","DOIUrl":"10.1016/j.mtbio.2024.101357","url":null,"abstract":"<div><div>Conventional antidepressants are slow to work and have serious side effects and poor response rates. As a precursor to 5-hydroxytryptamine (5-HT), 5-hydroxytryptophan (5-HTP) can be safely increased in concentration and rapidly metabolized into 5-HT in the brain, but the effectiveness of 5-HTP is severely limited due to its short half-life and lack of targeting. To traverse the blood-brain barrier (BBB) and achieve effective targeting, we designed a near-infrared (NIR) light-responsive artificial synaptic vesicles functionalized with an aptamer and loaded with 5-HTP and IR780. Photothermal approaches could improve the BBB permeability, and photothermal-triggered 5-HTP release could also be achieved. The ability to penetrate the BBB and enhance cerebral drug enrichment could be observed by fluorescence imaging. In addition, the nanoplatform incorporating the NIR laser considerably reduced depressive-like behaviors in chronic unpredictable moderate stress model mice in only 4 weeks, suggesting a potential approach for rapid-acting depression treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101357"},"PeriodicalIF":8.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoplatform for synergistic therapy constructed via the co-assembly of a reduction-responsive cholesterol-based block copolymer and a photothermal amphiphile","authors":"Yue Lu ,&nbsp;Tiantian Cai ,&nbsp;Juanjuan Gao ,&nbsp;Yangge Ren ,&nbsp;Yi Ding ,&nbsp;Shujing Liu ,&nbsp;Linyuan Liu ,&nbsp;Hao Huang ,&nbsp;Haijie Wang ,&nbsp;Chengji Wang ,&nbsp;Wei Wang ,&nbsp;Ruling Shen ,&nbsp;Bo Zhu ,&nbsp;Lin Jia","doi":"10.1016/j.mtbio.2024.101355","DOIUrl":"10.1016/j.mtbio.2024.101355","url":null,"abstract":"<div><div>The goal of combination cancer therapy, including chemo-phototherapy, is to achieve highly efficient antitumor effects while minimizing the adverse reactions associated with conventional chemotherapy. Nevertheless, enhancing the contribution of non-chemotherapeutic strategies in combination therapy is often challenging because this requires multiple active ingredients to be encapsulated in a single delivery system. However, most commonly used photothermal reagents are challenging to be loaded in large quantities and have poor biocompatibility. Herein, we developed photothermal co-micelles through a co-assembly strategy using a cholesterol-based liquid crystal block copolymer (LC-BCP) with disulfide bonds in the side chain of the LC blocks and a croconaine-based amphiphile (CBA) containing a cholesterol moiety. This approach allowed the CBA to be effectively embedded within LC-BCPs, serving as the functional component of the drug-loaded carrier. These co-micelles could encapsulate doxorubicin (DOX), showed tunable reduction-responsive drug release, and enabled near-infrared laser-triggered photothermal therapy as well as <em>in vivo</em> fluorescence and photothermal imaging. Following laser irradiation, the photothermal activity of the co-micelles rapidly induced tumor cell death and accelerated drug release. <em>In vitro</em> and <em>in vivo</em> experiments demonstrated that the synergistic photo-chemotherapeutic effects of these drug-loaded co-micelles offer a promising avenue for synergistic precision photothermal-chemotherapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101355"},"PeriodicalIF":8.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized adipose matrix hydrogel-based in situ delivery of antagomiR-150-5p for rat abdominal aortic aneurysm therapy","authors":"Xin Chen ,&nbsp;Shoushuai Wang ,&nbsp;Weijian Hou ,&nbsp;Yanhui Zhang ,&nbsp;Yapeng Hou ,&nbsp;Hao Tong ,&nbsp;Xiaoxin Zhang ,&nbsp;Yue Liu ,&nbsp;Ruoxuan Yang ,&nbsp;Xiang Li ,&nbsp;Qin Fang ,&nbsp;Jun Fan","doi":"10.1016/j.mtbio.2024.101350","DOIUrl":"10.1016/j.mtbio.2024.101350","url":null,"abstract":"<div><div>Abdominal aortic aneurysm (AAA) is a progressive aortic disease featured by inflammation, vascular smooth muscle cells (VSMCs) depletion, and elastin degradation. MicroRNAs were related to AAA formation, which bring the approach for precise and targeted drug therapy for AAA. We developed a new strategy based on decellularized adipose matrix (DAM) hydrogel immobilized on the adventitia to release antagomiR-150-5p for preventing the AAA development. In this study, Cacl₂-induced and elastase-induced rat AAA models were established. We found that miR-150-5p was upregulated while Notch3 was downregulated in two rat AAA models. Then a mold was designed for shaping hydrogel for miR-150-5p delivery around the abdominal aorta. Interestingly, inhibition of miR-150-5p in AAA by local release of antagomiR-150-5p with DAM hydrogel significantly prevented aortic dilation and elastin degradation. Moreover, inflammatory cell infiltration, the expression of inflammatory cytokines (MCP-1, TNF-α, and NF-κB (p65)), and matrix metalloproteinases (MMP-2, MMP-9) were increased while Notch3 and α-SMA were decreased in rat AAA, which can be attenuated by antagomiR-150-5p treatment. In VSMCs with TNF-α stimulation, we further demonstrated that inhibition of miR-150-5p downregulated NF-κB (p65), MMP-2, and MMP-9 and upregulated elastin via Notch3. This work presents a translational potential strategy for AAA repair via DAM hydrogel sustained release of antagomiR-150-5p, and highlights the mechanism of miR-150-5p during AAA progression by regulating Notch3.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101350"},"PeriodicalIF":8.7,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly stable and near-infrared responsive phase change materials for targeted enzyme delivery toward cancer therapy","authors":"Yuqiong Xia ,&nbsp;Chang Liu ,&nbsp;Xuejuan Zhao ,&nbsp;Keyun Wu ,&nbsp;Jianxia Cao ,&nbsp;Yutian Cao ,&nbsp;Cheng Zhu ,&nbsp;Xianghan Zhang","doi":"10.1016/j.mtbio.2024.101345","DOIUrl":"10.1016/j.mtbio.2024.101345","url":null,"abstract":"<div><div>Natural enzyme-based catalytic cascades have garnered increasing attention in cancer therapy, but their clinical utility is greatly limited due to loss of function during <em>in vivo</em> delivery. Here, we developed an enzyme delivering nanoplatform (GCI@RPCM) with great <em>in vivo</em> stability and achieve NIR-triggered enzyme dynamic therapy. This nanoplatform is created with encapsulation of nature enzymes (glucose oxidase and chloroperoxidase) and photothermal agent (indocyanine green) within tumor targeting and thermo-responsive phase change materials (RPCMs). With NIR irradiation for 10 min, GCI@RPCM can release 41 % of the enzymes and generate abundant reactive oxygen species (ROS), which showed significant tumor cell inhibition. After intravenous injection, GCI@RPCM can efficiently accumulate at the tumor site and local NIR treatment resulted in complete tumor eradication without detectable systemic toxicity. This study provides a highly stable and NIR-controllable smart delivery system and achieve enzyme dynamic therapy for enhanced breast cancer therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101345"},"PeriodicalIF":8.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering gene-activated bioprinted scaffolds for enhancing articular cartilage repair.","authors":"Min Wang, Jiachen Wang, Xin Xu, Erliang Li, Peng Xu","doi":"10.1016/j.mtbio.2024.101351","DOIUrl":"10.1016/j.mtbio.2024.101351","url":null,"abstract":"<p><p>Untreated articular cartilage injuries often result in severe chronic pain and dyskinesia. Current repair strategies have limitations in effectively promoting articular cartilage repair, underscoring the need for innovative therapeutic approaches. A gene-activated matrix (GAM) is a promising and comprehensive therapeutic strategy that integrates tissue-engineered scaffold-guided gene therapy to promote long-term articular cartilage repair by enhancing gene retention, reducing gene loss, and regulating gene release. However, for effective articular cartilage repair, the GAM scaffold must mimic the complex gradient structure of natural articular cartilage. Three-dimensional (3D) bioprinting technology has emerged as a compelling solution, offering the ability to precisely create complex microstructures that mimic the natural articular cartilage. In this review, we summarize the recent research progress on GAM and 3D bioprinted scaffolds in articular cartilage tissue engineering (CTE), while also exploring future challenges and development directions. This review aims to provide new ideas and concepts for the development of gene-activated bioprinted scaffolds with specific properties tailored to meet the stringent requirements of articular cartilage repair.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101351"},"PeriodicalIF":8.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MSCs-derived ECM functionalized hydrogel regulates macrophage reprogramming for osteoarthritis treatment by improving mitochondrial function and energy metabolism","authors":"Zhuolin Chen ,&nbsp;Qiming Pang ,&nbsp;Jingdi Zhan ,&nbsp;Junyan Liu ,&nbsp;Weikang Zhao ,&nbsp;Lili Dong ,&nbsp;Wei Huang","doi":"10.1016/j.mtbio.2024.101340","DOIUrl":"10.1016/j.mtbio.2024.101340","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a degenerative disease that affects the entire joint, with synovial inflammation being a major pathological feature. Macrophages, as the most abundant immune cells in the synovium, have an M1/M2 imbalance that is closely related to the occurrence and development of OA. Mesenchymal stem cells (MSCs) have been shown to effectively suppress inflammation in the treatment of OA, but they still pose issues such as immune rejection and tumorigenicity. The extracellular matrix (ECM), as a major mediator of MSCs' immunoregulatory effects, offers a cell-free therapy to circumvent these risks. In this study, we developed an ECM-functionalized hydrogel by combining MSC-derived ECM with gelatin methacryloyl (GelMA). To enhance the immunomodulatory potential of MSCs, we pre-stimulated MSCs with the inflammatory factor interleukin-6 (IL-6) present in OA. In vitro results showed that the ECM-functionalized hydrogel promoted M2 macrophage polarization and inhibited the expression of various inflammatory genes, strongly indicating the hydrogel's powerful immunoregulatory capabilities. In an in vivo rat OA model, the ECM-functionalized hydrogel significantly reduced synovial inflammation and cartilage matrix degradation, alleviating the progression of OA. Furthermore, we utilized proteomics and transcriptomics analysis to reveal that the hydrogel accomplished macrophage metabolic reprogramming by regulating mitochondrial function and energy metabolism, thereby reducing inflammation. These findings suggest that the ECM-functionalized hydrogel is a promising biomaterial-based strategy for treating OA by targeting key pathological mechanisms.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101340"},"PeriodicalIF":8.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing vaccine technology through the manipulation of pathogenic and commensal bacteria","authors":"Khristine Kaith S. Lloren,&nbsp;Amal Senevirathne,&nbsp;John Hwa Lee","doi":"10.1016/j.mtbio.2024.101349","DOIUrl":"10.1016/j.mtbio.2024.101349","url":null,"abstract":"<div><div>Advancements in vaccine technology are increasingly focused on leveraging the unique properties of both pathogenic and commensal bacteria. This revolutionary approach harnesses the diverse immune modulatory mechanisms and bacterial biology inherent in different bacterial species enhancing vaccine efficacy and safety. Pathogenic bacteria, known for their ability to induce robust immune responses, are being studied for their potential to be engineered into safe, attenuated vectors that can target specific diseases with high precision. Concurrently, commensal bacteria, which coexist harmlessly with their hosts and contribute to immune system regulation, are also being explored as novel delivery systems and in microbiome-based therapy. These bacteria can modulate immune responses, offering a promising avenue for developing effective and personalized vaccines. Integrating the distinctive characteristics of pathogenic and commensal bacteria with advanced bacterial engineering techniques paves the way for innovative vaccine and therapeutic platforms that could address a wide range of infectious diseases and potentially non-infectious conditions. This holistic approach signifies a paradigm shift in vaccine development and immunotherapy, emphasizing the intricate interplay between the bacteria and the immune systems to achieve optimal immunological outcomes.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101349"},"PeriodicalIF":8.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin carbon dots enhance neurogenesis and neuroprotection in Alzheimer's disease: A potential nanomedicine approach","authors":"Jing Zhang ,&nbsp;Xuehan Yang ,&nbsp;Sushan Wang ,&nbsp;Jianhua Dong ,&nbsp;Meishuang Zhang ,&nbsp;Ming Zhang ,&nbsp;Li Chen","doi":"10.1016/j.mtbio.2024.101347","DOIUrl":"10.1016/j.mtbio.2024.101347","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is characterized by progressive cognitive decline due to neuronal damage and impaired neurogenesis. Preserving neuronal integrity and stimulating neurogenesis are promising therapeutic strategies to combat AD-related cognitive dysfunction. In this study, we synthesized metformin carbon dots (CMCDs) using a hydrothermal method with metformin hydrochloride and citric acid as precursors. Notably, we found that CMCDs were significantly more effective than metformin in promoting the differentiation of neural stem cells (NSCs) into functional neurons under amyloid-beta (Aβ) conditions. Moreover, CMCDs fostered NSCs proliferation, enhanced neurogenesis, reduced Aβ deposition, and inhibited glial cell activation. We also examined neuronal structure by assessing Map2/NF-H/PSD95/SYN expression in the hippocampus, finding that CMCDs robustly strengthened neuronal structure. These results suggest that CMCDs can cognitive dysfunction in AD and promote the proliferation and neurogenesis of NSCs, as well as ameliorate neuronal injury. Hence, CMCDs emerge as promising candidates for AD therapy, demonstrating superior efficacy compared to metformin alone, and offering novel insights into small molecule drug interventions for AD.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101347"},"PeriodicalIF":8.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}