Materials Today Bio最新文献

{"title":"Nanoscale piezoelectric patches preserve electrical integrity of infarcted hearts","authors":"Luís M. Monteiro ,&nbsp;Pedro J. Gouveia ,&nbsp;Francisco Vasques-Nóvoa ,&nbsp;Susana Rosa ,&nbsp;Ifigeneia Bardi ,&nbsp;Rita N. Gomes ,&nbsp;Simão Correia-Santos ,&nbsp;Leonardo Ricotti ,&nbsp;Lorenzo Vannozzi ,&nbsp;Daniele Guarnera ,&nbsp;Liliana Costa ,&nbsp;André M. Leite-Moreira ,&nbsp;Pedro Mendes-Ferreira ,&nbsp;Adelino F. Leite-Moreira ,&nbsp;Filippo Perbellini ,&nbsp;Cesare M. Terracciano ,&nbsp;Perpétua Pinto-do-Ó ,&nbsp;Lino Ferreira ,&nbsp;Diana S. Nascimento","doi":"10.1016/j.mtbio.2025.101742","DOIUrl":"10.1016/j.mtbio.2025.101742","url":null,"abstract":"<div><div>Ischemic heart disease is the leading cause of death worldwide. Several approaches have been explored to restore cardiac function, however few investigated new strategies to improve electrical functional recovery. Herein, we have investigated the impact of piezoelectric patches (Piezo patches), capable of generating electric charges upon mechanical deformation, on rat cardiac slices, healthy and ischemic hearts (<em>ex vivo</em>), on infarcted mice (<em>in vivo</em>) and on healthy and infarcted pigs (<em>in vivo</em>). Piezo patches did not preclude cardiac slice contractility, while compared with electrically inert control patches. In addition, Piezo patches showed an adequate safety profile in a working heart model as no electrophysiologic alterations were detected in healthy hearts. Epicardial implantation of Piezo patches in acutely infarcted mice hearts significantly improved myocardial electrical integrity without disturbing systolic function. Moreover, Piezo patches partially prevented ischemia-related adverse cardiac remodeling, reducing left ventricular chamber dilatation and compensatory hypertrophy. Coherently, Piezo patch-implanted hearts revealed downregulation of genes associated with extracellular matrix remodeling. Importantly, <em>in vivo</em> implantation of Piezo patches in porcine hearts revealed to be electrically safe as no major effects in its electrophysiology were detected. Overall, the results presented here endorse Piezo patches as a promising therapeutic strategy to improve post-myocardial infarction structural and electrical remodeling.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101742"},"PeriodicalIF":8.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH/Hyal-responsive vancomycin-loaded chitooligosaccharide nanoparticles for intracellular MRSA infection treatment","authors":"Wenting Li ,&nbsp;WeiWei Li ,&nbsp;Xuanxiang Zhai,&nbsp;Xiao Liu,&nbsp;Xiaoyi Shi,&nbsp;Xiangjun Chen,&nbsp;Wei Hong","doi":"10.1016/j.mtbio.2025.101731","DOIUrl":"10.1016/j.mtbio.2025.101731","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>) is recognized as among the most critical bacterial pathogens globally. A significant portion of the complications associated with <em>S. aureus</em> infections arises from its ability to persist inside host phagocytes, particularly macrophages, making the eradication of intracellular <em>S. aureus</em> vital for therapeutic success. Regrettably, many antibiotics exhibit limited penetration into cells, underscoring the necessity for efficient intracellular delivery mechanisms. In this study, vancomycin-loaded chitooligosaccharide nanoparticles (COS@Van) coated with hyaluronic acid (HA), were engineered to function as an active-targeting antibiotic carrier recorded as HA/COS@Van. The HA coating serves as an external shell, which 1) covers the positive surface charge of COS NPs, thereby enhancing their biocompatibility and extending circulation time, and 2) facilitates targeted delivery to macrophages through specific interactions with the CD44 receptor. Confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) experiments confirmed that HA/COS could effectively accumulate in methicillin-resistant <em>S. aureus</em> (MRSA) infected macrophages. Additionally, when administered intravenously in mouse models, HA/COS demonstrated markedly increased accumulation in the liver, the primary location of infected macrophages. These findings highlight the active-targeting capabilities of HA/COS both <em>in vitro</em> and <em>in vivo</em> settings. Consequently, after being loaded with Van, HA/COS@Van exhibited superior efficacy in killing intracellular MRSA <em>in vitro</em>, as compared to free Van. Furthermore, HA/COS@Van also demonstrated enhanced bactericidal activity in both mouse acute peritonitis model and mouse organ infection model. Therefore, this active-targeting delivery system may hold promise in advancing therapeutic outcomes for infections related to intracellular pathogens.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101731"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bi-phasic integrated silk fibroin/polycaprolactone scaffolds for osteochondral regeneration inspired by the native joint tissue and interface","authors":"Zexing Zhang ,&nbsp;Qingquan Dong ,&nbsp;Zubing Li ,&nbsp;Gu Cheng ,&nbsp;Zhi Li","doi":"10.1016/j.mtbio.2025.101737","DOIUrl":"10.1016/j.mtbio.2025.101737","url":null,"abstract":"<div><div>Osteochondral scaffolds designed with bi-phasic and multi-phasic have typically struggled with post-implantation delamination. To address this issue, we developed a novel integrated scaffold with natural and continuous interface and heterogeneous bilayer structure. Through layer-by-layer wet electrospinning, two-dimensional (2D) bi-layer integrated membranes of silk fibroin (SF) and polycaprolactone (PCL) were fabricated. These membranes were then transformed into three-dimensional (3D) scaffolds using a CO₂ gas foaming technique, followed by gelatin coating on the osteogenic layer to afford final bi-phasic porous scaffolds. <em>In vitro</em> studies indicated that the 3D scaffolds better-maintained cell phenotypes than conventional 2D electrospun films. Additionally, the 3D scaffolds showed superior cartilage repair and osteoinductivity potential, with increased subchondral bone volume and reduced defect area in rat osteochondral defects models at 12 weeks. Taken together, these gas-foamed scaffolds were a promising candidate for osteochondral regeneration.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101737"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A vascularized tumors-on-a-chip model for studying tumor-angiogenesis interplay, heterogeneity and drug responses","authors":"Suyeon Shin ,&nbsp;Yurim Choi ,&nbsp;WonJun Jang ,&nbsp;Batjargal Ulziituya ,&nbsp;Giheon Ha ,&nbsp;Raehui Kang ,&nbsp;Soojin Park ,&nbsp;Minseok Kim ,&nbsp;Yu Shrike Zhang ,&nbsp;Han-Jun Kim ,&nbsp;Junmin Lee","doi":"10.1016/j.mtbio.2025.101741","DOIUrl":"10.1016/j.mtbio.2025.101741","url":null,"abstract":"<div><div>Current tumor models struggle to replicate the complexity of the tumor microenvironment, particularly endothelial sprouting and vascular-tumor interactions. To address these limitations, we developed a vascularized tumors-on-a-chip model by fusing tumor spheroids with HUVEC spheroids to simulate angiogenesis. The model incorporates hypoxia-driven cytokine secretion and dynamic endothelial penetration, enabling accurate recapitulation of angiogenic processes. Spheroids were optimized for size and viability, and four cancer types were studied, with GBM and A549 exhibiting the highest angiogenic potential, as confirmed by Z-stack imaging and qRT-PCR. Encapsulation in GelMA and integration into PDMS-based microfluidic chips provided a dynamic flow environment, mimicking <em>in vivo</em> drug delivery while enabling high-throughput drug screening. This chip-based system allows simultaneous testing of multiple drugs or tumors under physiologically relevant conditions, enhancing its translational potential. The platform was validated using doxorubicin and bevacizumab, revealing reduced VEGF secretion and dynamic cytokine responses, replicating vascular barriers. Further validation in murine models demonstrated its capacity to promote angiogenesis and mimic tumor-vessel interactions. This advanced tumors-on-a-chip model addresses critical shortcomings of conventional 2D and 3D systems and offers a transformative tool for preclinical drug evaluation and the development of precision oncology strategies, bridging the gap between <em>in vitro</em> testing and <em>in vivo</em> relevance.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101741"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted biomimetic MOF-functionalized hydrogel scaffolds for bone regeneration: Synergistic osteogenesis and osteoimmunomodulation","authors":"San-yang Yu ,&nbsp;Ting Wu ,&nbsp;Kai-hao Xu ,&nbsp;Ru-yue Liu ,&nbsp;Tian-hao Yu ,&nbsp;Zhen-hua Wang ,&nbsp;Zhong-ti Zhang","doi":"10.1016/j.mtbio.2025.101740","DOIUrl":"10.1016/j.mtbio.2025.101740","url":null,"abstract":"<div><div>Critical-size bone defects remain a significant clinical challenge. The lack of endogenous stem cells with osteogenic differentiation potential in the defect area, combined with the inflammatory responses induced by scaffold implantation, highlights the need for biomaterials that can deliver stem cells and possess inflammatory regulation properties. In this study, we developed a 3D bioprinted gelatin methacrylate (GelMA) hydrogel scaffold modified with luteolin-loaded ZIF-8 (LUT@ZIF-8) nanoparticles, designed to deliver bone marrow mesenchymal stem cells (BMSCs) to the defect site and release bioactive components that promote osteogenesis and modulate the immune microenvironment. The LUT@ZIF-8/GelMA hydrogel scaffolds demonstrated excellent physical properties and biocompatibility. The sustained release of luteolin and zinc ions from the LUT@ZIF-8 nanoparticles conferred antibacterial, osteoinductive, and inflammatory regulation effects. The immune microenvironment modulated by LUT@ZIF-8/GelMA hydrogel scaffolds facilitated osteogenic differentiation of BMSCs. Furthermore, <em>in vivo</em> experiments confirmed the osteogenic and inflammatory regulation capabilities of the LUT@ZIF-8/GelMA hydrogel scaffolds. In conclusion, the 3D bioprinted LUT@ZIF-8/GelMA hydrogel scaffolds exhibit osteoimmunomodulatory properties, presenting a promising strategy for the treatment of bone defects.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101740"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-targeting nanomaterials based on metal-organic frameworks mediate tumor immunotherapy by promoting cuproptosis and pyroptosis in hepatocellular carcinoma cells","authors":"Xiaoyuan Yi ,&nbsp;Huaying Xie ,&nbsp;Kunzhao Huang ,&nbsp;Jianzhang Luo ,&nbsp;Wen Li ,&nbsp;Qingyu Zeng ,&nbsp;Feifei He ,&nbsp;Wuxiang Shi ,&nbsp;Duo Wang ,&nbsp;Liyan Wang","doi":"10.1016/j.mtbio.2025.101745","DOIUrl":"10.1016/j.mtbio.2025.101745","url":null,"abstract":"<div><div>Activating a robust immune response is an effective strategy for achieving tumor eradication. In this study, copper-based metal-organic framework nanoparticles (referred to as FA-PZ@MOF NPs), which have the ability to simultaneously induce cuproptosis and pyroptosis in tumor cells, were designed to utilize the synergistic effects of cuproptosis and pyroptosis to trigger immunogenic cell death (ICD). This can inhibit tumor growth, migration, and metastasis while enabling efficient antitumor immunotherapy. The nano inducer targets hepatocellular carcinoma tumor cells via folic acid, dissociates and releases copper ions in the tumor microenvironment (TME), which has a high glutathione concentration, leading to copper ion overload, thereby mediating cuproptosis. Additionally, the released ZnO₂ generates substantial amounts of H₂O₂ and Zn²⁺ in the acidic environment, enhancing the Cu²⁺-based Fenton-like reaction for chemokinetic therapy. This exacerbates the reactive oxygen species (ROS) storms and mitochondrial damage, and combined with the action of Polyphyllin VI (PPVI), induces pyroptosis and cuproptosis. This multilayered interaction strategy also triggers robust ICD while inhibiting hepatocellular carcinoma tumor metastasis and invasion.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101745"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in abiotic tissue-based biomaterials: A focus on decellularization and devitalization techniques","authors":"Diana F. Tavares,&nbsp;João F. Mano,&nbsp;Mariana B. Oliveira","doi":"10.1016/j.mtbio.2025.101735","DOIUrl":"10.1016/j.mtbio.2025.101735","url":null,"abstract":"<div><div>This Review explores the growing and diversifying field of tissue-derived abiotic constructs for tissue engineering applications, with main focus on decellularization and devitalization techniques and principles. Acellular fractions derived from biological tissues, such as the extracellular matrix (ECM), have long been considered a valuable approach for the generation of numerous scaffolds and more complex constructs. The removal of the cellular content has been considered essential to prevent the development of adverse immunological reactions. Nevertheless, the discovery of promising features of certain cellular components has sparked interest in the use of inactivated or devitalized cellular fractions for several applications, particularly in regenerative medicine and inflammation control. Devitalization has been described for several clinical applications, but remains poorly explored in terms of in vitro constructs compared to decellularization methods currently available. In this review, we present and critically evaluate a spectrum of approaches for the decellularization of whole-organs and in vitro constructs, and the most prevalent devitalization techniques, with a discussion on their implications on scaffolds composition, structure, and potentially therapeutic properties. Processing methodologies to achieve optimal cell-based abiotic materials and approaches for their effective characterization are described and discussed. The application of these materials in healthcare, with most focus on regenerative approaches and including examples of commercially available products, is also addressed.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101735"},"PeriodicalIF":8.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-responsive nanovesicles capable of remodeling the tumor microenvironment enable activatable near-infrared-II fluorescence image-guided enhanced radiotherapy","authors":"Lin Zhao ,&nbsp;Mengzhen Wang ,&nbsp;Yang Sun ,&nbsp;Jinpeng Xu ,&nbsp;Qinrui Fu ,&nbsp;Wenjing Xiao","doi":"10.1016/j.mtbio.2025.101725","DOIUrl":"10.1016/j.mtbio.2025.101725","url":null,"abstract":"<div><div>Traditional radiotherapy (RT) lacks the precision to distinguish between tumor and normal tissues, leading to inevitable X-ray-induced side effects in patients. Therefore, it is crucial to develop integrated imaging and therapeutic modalities that can reduce side effects on surrounding healthy tissues while enhancing susceptibility to tumor tissues. In this study, we developed a pH-responsive nanodrug (AuNRs-Mn₃O₄-Ag₂S Ve) by self-assembling the second near-infrared (NIR-II, 950–1700 nm) fluorescent probe Ag₂S quantum dots (QDs), multifunctional nanozyme Mn₃O₄ nanoparticles (NPs), and radiosensitizer gold nanorods (AuNRs) into a single nanoplatform <em>via</em> an emulsion process. This nanodrug enables precise tumor localization for accurately guided RT and multi-angle sensitization of RT. Upon intravenous administration, the nanodrug disintegrates in the tumor area due to the pH-sensitive polymer P4VP, releasing Ag₂S QDs which are specifically activated by the acidic environment, thereby “turning on” the NIR-II fluorescence signal. The optimal timing of the NIR-II fluorescence signal within the tumor region after intravenous injection was investigated, providing a reference for guided RT. <em>In vitro</em> and <em>in vivo</em> experiments confirmed the efficient enhancement of tumor radiosensitization by AuNRs and Mn₃O₄ NPs. The specific imaging modality that transitions the fluorescence signal from “off” to “on” has been successfully implemented, addressing the limitations of conventional RT and enhancing radiosensitivity. The integration of imaging and therapeutic approaches in this study presents a promising modality for image-guided tumor RT.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101725"},"PeriodicalIF":8.7,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in biomimetic nanodelivery systems for cancer Immunotherapy","authors":"Jiawei Yang ,&nbsp;Xueqi Li ,&nbsp;Tongyu Li ,&nbsp;Jin Mei ,&nbsp;Ying Chen","doi":"10.1016/j.mtbio.2025.101726","DOIUrl":"10.1016/j.mtbio.2025.101726","url":null,"abstract":"<div><div>Tumor immunotherapy is a developing and promising therapeutic method. However, the mechanism of tumor immune microenvironment and individual differences of patients make the clinical application of immunotherapy still very limited. The resulting targeting of the tumor environment and immune system is a suitable strategy for tumor therapy. Biomimetic nanodelivery systems (BNDS) coated with nanoparticles has brought new hope for tumor immunotherapy. Due to its high targeting, maximum drug delivery efficiency and immune escape, BNDS has become one of the options for tumor immunotherapy in the future. BNDS combines the advantages of natural cell membranes and nanoparticles and has good targeting properties. This review summarizes the relationship between tumor and immune microenvironment, classification of immunotherapy, engineering modification of cell membrane, and a comprehensive overview of different types of membrane BNDS in immunotherapy. Furthermore, the prospects and challenges of biomimetic nanoparticles coated with membranes in tumor immunotherapy are further discussed.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101726"},"PeriodicalIF":8.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel anti-pyroptosis drug loaded on metal-organic framework for intervertebral disc degeneration therapy","authors":"Yekai Zhang ,&nbsp;Jiawei Qiu ,&nbsp;Yiji Chen ,&nbsp;Yu Chen ,&nbsp;Xiaopeng Liu ,&nbsp;Hanwen Zhang ,&nbsp;Hualin Li ,&nbsp;Kaiyu Li ,&nbsp;Haobo Ye ,&nbsp;Yaosen Wu ,&nbsp;Xiaolei Zhang ,&nbsp;Naifeng Tian","doi":"10.1016/j.mtbio.2025.101729","DOIUrl":"10.1016/j.mtbio.2025.101729","url":null,"abstract":"<div><div>Intervertebral disc degeneration (IVDD) is the main cause of low back pain, pyroptosis is a major contributor to various diseases, including IVDD; however, there is currently no effective drugs targeting pyroptosis for therapy. In this study, we established pyroptosis model in nucleus pulposus cells (NPCs) <em>in vitro</em> and searched pyroptosis inhibitors in FDA Medicine Library. High throughput screening study revealed that Pirfenidone (PFD) was the most effective pyroptosis inhibitor among 1500+ FDA drugs, which was confirmed by further experiments. As administering PFD alone may lead to poor efficacy due to short action time and low bioavailability, we designed a smart delivery system for PFD. A pH-responsive metal-organic framework (MOF), poly-His6-zinc (PHZ) assembly, loaded with PFD (PFD@PHZ) was designed for IVDD therapy. PHZ was shown to have excellent lysosomal escape properties and bioavailability of PFD. In addition, the release of PDF from PFD@PHZ could be triggered by the acidic microenvironment of degenerated intervertebral discs. PFD@PHZ was also shown to effectively inhibit pyroptosis, senescence, and extracellular matrix (ECM) degradation in NPCs, both <em>in vitro</em> and <em>in vivo</em>, thereby mitigating the progression of IVDD in rats. Thus, the current study shows PFD as a novel inhibitor for pyroptosis, and PFD@PHZ as a potential nanomaterial for efficient IVDD therapy.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101729"},"PeriodicalIF":8.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}