Materials Today Bio最新文献

{"title":"High paracrine activity of hADSCs cartilage microtissues inhibits extracellular matrix degradation and promotes cartilage regeneration","authors":"Wei Liu, Hongyu Jiang, Jiajie Chen, Yue Tian, Ying He, Ying Jiao, Yanjun Guan, Zhibo Jia, Yanbin Wu, Cheng Huang, Yiben Ouyang, Wenjing Xu, Jianhong Qi, Jiang Peng, Aiyuan Wang","doi":"10.1016/j.mtbio.2024.101372","DOIUrl":"https://doi.org/10.1016/j.mtbio.2024.101372","url":null,"abstract":"Due to its unique structure, articular cartilage has limited self-repair capacity. Microtissues are tiny tissue clusters that can mimic the function of target organs or tissues. Using cells alone for microtissue construction often results in the formation of necrotic cores. However, the extracellular matrix (ECM) of native cartilage can provide structural support and is an ideal source of microcarriers. Autologous adipose-derived mesenchymal stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) are widely used in cartilage tissue engineering. In this study, we fabricated microcarriers and compared the behavior of two homologous cell types in the microcarrier environment. The microcarrier environment highlighted the advantages of ADSCs and promoted the proliferation and migration of these cells. Then, ADSCs microtissues (ADSCs-MT) and BMSCs microtissues (BMSCs-MT) were fabricated using a three-dimensional dynamic culture system. In vitro and in vivo experiments verified that the cartilage regeneration ability of ADSCs-MT was significantly superior to that of BMSCs-MT. Transcriptomics revealed that ADSCs-MT showed significantly lower expression levels of ECM degradation, osteogenesis, and fibrocartilage markers. Finally, the protective effect of microtissues on inflammatory chondrocytes was validated. Overall, the ADSCs-MT constructed in this study achieved excellent cartilage regeneration and could be promising for the autologous application of cartilage microtissues.","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"13 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformation gap from research findings to large-scale commercialized products in microfluidic field.","authors":"Yuqi Ma, Xiaoyi Sun, Ziwei Cai, Mengjing Tu, Yugang Wang, Qi Ouyang, Xueqing Yan, Gaoshan Jing, Gen Yang","doi":"10.1016/j.mtbio.2024.101373","DOIUrl":"https://doi.org/10.1016/j.mtbio.2024.101373","url":null,"abstract":"<p><p>The field of microfluidics has experienced rapid growth in the last several decades, yet it isn't considered to be a large industry comparable to semiconductor and consumer electronics. In this review, we analyzed the entire process of the transformation from research findings to commercialized products in microfluidics, as well as the significant gap during the whole developing process between microchip fabrication in R&D and large-scale production in the industry. We elaborated in detail on various materials in the microfluidics industry, including silicon, glass, PDMS, and thermoplastics, discussing their characteristics, production processes, and existing products. Despite challenges hindering the large-scale commercialization of microfluidic chips, ongoing advancements and applications are expected to integrate microfluidic technology into everyday life, transforming it into a commercially viable field with substantial potential and promising prospects.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101373"},"PeriodicalIF":8.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11647665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements and clinical aspects of engineered iron oxide nanoplatforms for magnetic hyperthermia-induced cancer therapy.","authors":"Arunima Rajan, Suvra S Laha, Niroj Kumar Sahu, Nanasaheb D Thorat, Balakrishnan Shankar","doi":"10.1016/j.mtbio.2024.101348","DOIUrl":"10.1016/j.mtbio.2024.101348","url":null,"abstract":"<p><p>The pervasiveness of cancer is a global health concern posing a major threat in terms of mortality and incidence rates. Magnetic hyperthermia (MHT) employing biocompatible magnetic nanoparticles (MNPs) ensuring selective attachment to target sites, better colloidal stability and conserving nearby healthy tissues has garnered widespread acceptance as a promising clinical treatment for cancer cell death. In this direction, multifunctional iron oxide nanoparticles (IONPs) are of significant interest for improved cancer care due to finite size effect associated with inherent magnetic properties. This review offers a comprehensive perception of IONPs-mediated MHT from fundamentals to clinical translation, by elucidating the underlying mechanism of heat generation and the related influential factors. Biological mechanisms underlying MHT-mediated cancer cell death such as reactive oxygen species generation and lysosomal membrane permeabilization have been discussed in this review. Recent advances in biological interactions (in vitro and in vivo) of IONPs and their translation to clinical MHT applications are briefed. New frontiers and prospects of promising combination cancer therapies such as MHT with photothermal therapy, cancer starvation therapy and sonodynamic therapy are presented in detail. Finally, this review concludes by addressing current crucial challenges and proposing possible solutions to achieve clinical success.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101348"},"PeriodicalIF":8.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monosaccharide coating modulate the intracellular trafficking of gold nanoparticles in dendritic cells.","authors":"Meshal A Alobaid, Sarah-Jane Richards, Morgan R Alexander, Matthew I Gibson, Amir M Ghaemmaghami","doi":"10.1016/j.mtbio.2024.101371","DOIUrl":"https://doi.org/10.1016/j.mtbio.2024.101371","url":null,"abstract":"<p><p>Dendritic cells (DCs) have emerged as a promising target for drug delivery and immune modulation due to their pivotal role in initiating the adaptive immune response. Gold nanoparticles (AuNPs) have garnered interest as a platform for targeted drug delivery due to their biocompatibility, low toxicity and precise control over size, morphology and surface functionalization. Our investigation aimed to elucidate the intracellular uptake and trafficking of AuNPs coated with different combinations of monosaccharides (mannose, galactose, and fucose) in DCs. We used 30 unique polymer-tethered monosaccharide combinations to coat 16 nm diameter spherical gold nanoparticles and investigated their effect on DCs phenotype, uptake, and intracellular trafficking. DCs internalized AuNPs coated with 100 % fucose, 100 % mannose, 90 % mannose +10 % galactose, and 80 % mannose +20 % galactose with highest efficiency. Flow cytometry analysis indicated that 100 % fucose-coated AuNPs showed increased lysosomal and endosomal contents compared to other conditions and uncoated AuNPs. Imaging flow cytometry further demonstrated that 100 % fucose-coated AuNPs had enhanced co-localization with lysosomes, while 100 % mannose-coated AuNPs exhibited higher co-localization with endosomes. Furthermore, our data showed that the uptake of carbohydrate-coated AuNPs predominantly occurred through receptor-mediated endocytosis, as evidenced by a marked reduction of uptake upon treatment of DCs with methyl-β-cyclodextrins, known to disrupt receptor-mediated endocytosis. These findings highlight the utility of carbohydrate coatings to enable more targeted delivery of nanoparticles and their payload to distinct intracellular compartments in immune cells with potential applications in drug delivery and immunotherapy.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101371"},"PeriodicalIF":8.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy","authors":"Kaixuan Ma ,&nbsp;Lei Yang ,&nbsp;Wenzhao Li ,&nbsp;Kai Chen ,&nbsp;Luoran Shang ,&nbsp;Yushu Bai ,&nbsp;Yuanjin Zhao","doi":"10.1016/j.mtbio.2024.101363","DOIUrl":"10.1016/j.mtbio.2024.101363","url":null,"abstract":"<div><div>Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there is still room for improvement regarding the component of the microspheres to achieve sufficient bioactivity. Here, we prepare multi-bioactive microspheres composed of methacrylated silk fibroin (SFMA) <em>via</em> microfluidic electrospray. Magnesium ascorbyl phosphate (MAP) is encapsulated within the microspheres, whose sustained release facilitates angiogenesis and osteogenic differentiation. The microspheres are further coated with a polydopamine (PDA) layer, allowing them to assemble <em>in</em> <em>situ</em> into a scaffold that conforms to the non-uniform contours of bone defects. The photothermal conversion capability of PDA also provides mild photothermal stimulation to further promote bone regeneration. Based on the synergistic effects, our <em>in vivo</em> experiments demonstrated that the microsphere scaffold effectively promotes bone defect healing. Thus, this multi-bioactive scaffold offers a versatile strategy for bone repair with promising clinical potential.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101363"},"PeriodicalIF":8.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging printability and biocompatibility in materials for printing implantable vessel scaffolds.","authors":"Tianhong Chen, Haihong Jiang, Ruoxuan Zhang, Fan He, Ning Han, Zhimin Wang, Jia Jia","doi":"10.1016/j.mtbio.2024.101366","DOIUrl":"https://doi.org/10.1016/j.mtbio.2024.101366","url":null,"abstract":"<p><p>Vessel scaffolds are crucial for treating cardiovascular diseases (CVDs). It is currently feasible to fabricate vessel scaffolds from a variety of materials using traditional fabrication methods, but the risks of thrombus formation, chronic inflammation, and atherosclerosis associated with these scaffolds have led to significant limitations in the clinical usages. Bioprinting, as an emerging technology, has great potential in constructing implantable vessel scaffolds. During the fabrication of the constructs, the biomaterials used for bioprinting have offered significant contributions for the successful fabrications of the vessel scaffolds. Herein, we review recent advances in biomaterials for bioprinting implantable vessel scaffolds. First, we briefly introduce the requirements for implantable vessel scaffolds and its conventional manufacturing methods. Next, a brief overview of the classic methods for bioprinting vessel scaffolds is presented. Subsequently, we provide an in-depth analysis of the properties of the representative natural, synthetic, composite and hybrid biomaterials that can be used for bioprinting implantable vessel scaffolds. Ultimately, we underscore the necessity of leveraging biocompatibility and printability for biomaterials, and explore the unmet needs and potential applications of these biomaterials in the field of bioprinted implantable vessel scaffolds.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101366"},"PeriodicalIF":8.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress of paclitaxel nanodrug delivery system in the treatment of triple-negative breast cancer.","authors":"Jia-Xin Qiao, Dong-Yan Guo, Huan Tian, Zhan-Peng Wang, Qiang-Qiang Fan, Yuan Tian, Jing Sun, Xiao-Fei Zhang, Jun-Bo Zou, Jiang-Xue Cheng, Fei Luan, Bing-Tao Zhai","doi":"10.1016/j.mtbio.2024.101358","DOIUrl":"10.1016/j.mtbio.2024.101358","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, characterized by the loss or low expression of estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2) and progesterone receptor (PR). Due to the lack of clear therapeutic targets, paclitaxel (PTX) is often used as a first-line standard chemotherapy drug for the treatment of high-risk and locally advanced TNBC. PTX is a diterpenoid alkaloid extracted and purified from Taxus plants, functioning as an anticancer agent by inducing and promoting tubulin polymerization, inhibiting spindle formation in cancer cells, and preventing mitosis. However, its clinical application is limited by low solubility and high toxicity. Nanodrug delivery system (NDDS) is one of the feasible methods to improve the water solubility of PTX and reduce side effects. In this review, we summarize the latest advancements in PTX-targeted NDDS, as well as its combination with other codelivery therapies for TNBC treatment. NDDS includes passive targeting, active targeting, stimuli-responsive, codelivery, and multimode strategies. These systems have good prospects in improving the bioavailability of PTX, enhancing tumor targeting, reducing toxicity, controlling drug release, and reverse tumor multidrug resistance (MDR). This review provides valuable insights into the clinical development and application of PTX-targeted NDDS in the treatment of TNBC.</p>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"101358"},"PeriodicalIF":8.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable hyaluronate-L- cysteine gel potentiates photothermal therapy in osteosarcoma via vorinostat-copper cell death","authors":"Sizhen Wang ,&nbsp;Hanzhe Zhang ,&nbsp;Tianheng Chen ,&nbsp;Weiwei Jiang ,&nbsp;Feng Wang ,&nbsp;Yuhao Yu ,&nbsp;Beibei Guo ,&nbsp;Jia Xu ,&nbsp;Feng Yang ,&nbsp;Qinglin Kang ,&nbsp;Zhiqiang Ma","doi":"10.1016/j.mtbio.2024.101368","DOIUrl":"10.1016/j.mtbio.2024.101368","url":null,"abstract":"<div><div>The prognosis for osteosarcoma patients, a devastating malignancy affecting young individuals, remains grim despite multimodal therapeutic advances. Recently, the advent of cuproptosis, a novel programmed cell death, offers hope in fighting osteosarcoma. In this study, we introduce SAHA_m@{[Cu(HA-Cys)₂]Cl₂}_n, an injectable hyaluronate-L-cysteine hydrogel that integrates both copper ions (Cu²⁺) and vorinostat (SAHA) for the possible therapeutic effect. The Cu²⁺ targets the TCA cycle, inducing cuproptosis in osteosarcoma cells. While SAHA acts as both a histone deacetylase inhibitor and an ROS generator for eliminating tumor cells. The mechanism involves amplifying FDX-1 expression via SAHA modulation of the TCA cycle, which was an original discovery. Critically, the combined mechanisms and localized injection enables the hydrogel partially eradicating osteosarcoma without metastasis in rats. Therefore, this study advances cuproptosis induced photothermal therapy for promising clinical translations, shedding light on favorable prognosis for osteosarcoma.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101368"},"PeriodicalIF":8.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries","authors":"Hang Chen ,&nbsp;Lu Tan ,&nbsp;Liqi Li ,&nbsp;Yan Zheng ,&nbsp;Menghuan Li ,&nbsp;Shuohan He ,&nbsp;Zhong Luo ,&nbsp;Kaiyong Cai ,&nbsp;Yan Hu","doi":"10.1016/j.mtbio.2024.101359","DOIUrl":"10.1016/j.mtbio.2024.101359","url":null,"abstract":"<div><div>Thermal burn injuries induce substantial alterations in the immune compositions and anatomical structures in the skin, which are characterized by strong inflammatory responses and thick eschar formation on the wound surface. These traits challenge current treatment paradigms due to insufficient drug penetration into affected tissues and the unsatisfactory wound regeneration. Herein, we report a layered microneedle (MN) patch for addressing these challenges in burn injury healing. The MN patch features a core/shell structure with methacrylated gelatin (GelMA) encapsulated with human umbilical vein endothelial cell (HUVECs)-derived hypoxia-induced exosomes (EXO-H) as the bottom layer and sodium alginate (SA) containing naringin (Nar)-loaded CaCO₃ nanoparticles (CaCO₃@Nar) as the top layer. Upon administration onto thermal burn injury site, the MN patches enable transdermal drug delivery by perforating the eschar. The spontaneous degradation of CaCO₃@Nar in the interstitial fluid triggers sustained Nar release to alleviate local inflammation and scavenge excessive reactive oxygen species (ROS). Meanwhile, EXO-H significantly promote the migration and proliferation of HUVECs and enhance their angiogenesis capacity to support scarless wound tissue regeneration. The MN patch in this work successfully promoted scarless healing of skin burn injuries on rat models, providing an approach for thermal burn treatment in the clinics.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101359"},"PeriodicalIF":8.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anisotropic patterns of nanospikes induces anti-biofouling and mechano-bactericidal effects of titanium nanosurfaces with electrical cue","authors":"Eiji Kato ,&nbsp;Masahiro Yamada ,&nbsp;Eitoyo Kokubu ,&nbsp;Hiroshi Egusa ,&nbsp;Kazuyuki Ishihara","doi":"10.1016/j.mtbio.2024.101352","DOIUrl":"10.1016/j.mtbio.2024.101352","url":null,"abstract":"<div><div>Anti-microbial nanopatterns have attracted considerable attention; however, its principle is not yet fully understood, particularly for inorganic nanopatterns. Titanium nanosurfaces with dense and anisotropically patterned nanospikes regulate biological functions with multiple physical stimulations, which may be because of the nanopattern-induced alternation of surface physical properties. This study aimed to determine the antimicrobial capability of titanium nanosurfaces and their mechanisms. Two types of alkali-etched titanium nanosurfaces with isotropically or anisotropically patterned nanospikes had markedly denser surface protrusions, greater superhydrophilicity, and greater negative charge than machined or micro-roughened titanium surfaces. The crystallographic properties of anisotropic titanium nanosurfaces were similar to those of isotropic nanosurfaces, but markedly higher in electric reactivity at nanoscale. The maximum value of the contact potential difference on titanium surfaces was significantly correlated with the product of the density and anisotropy in the distribution pattern of surface protrusions. Isotropic titanium nanosurfaces did not inhibit the attachment of gram-positive cocci, such as <em>Staphylococcus aureus</em>, whereas anisotropic titanium nanosurfaces substantially inhibited gram-positive cocci attachment. Most gram-negative bacilli, <em>Escherichia coli</em>, died via swelling of the cell body on anisotropic titanium nanosurfaces within 6 h of incubation, in contrast to other titanium surfaces where most of the cells did not lose viability or undergo morphological changes. The extent of cell swelling was positively correlated with the electric reactivity of the titanium surfaces. Titanium nanosurfaces with anisotropically patterned dense nanospikes exerted anti-biofouling or mechano-bactericidal effects on gram-positive or negative bacteria with electrical cue induced by the anisotropy of the nanospike patterns.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"29 ","pages":"Article 101352"},"PeriodicalIF":8.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}