Materials Today Bio最新文献

{"title":"A novel strategy for bone defect repair: Stromal cell-derived factor 1α sustained-release acellular fish scale scaffolds combined with injection of bone marrow mesenchymal stem cells promote bone regeneration","authors":"Shilong Su ,&nbsp;Jinwu Bai ,&nbsp;Ruideng Wang ,&nbsp;Shan Gao ,&nbsp;Rubing Zhou ,&nbsp;Fang Zhou","doi":"10.1016/j.mtbio.2025.101759","DOIUrl":"10.1016/j.mtbio.2025.101759","url":null,"abstract":"<div><div>Patients with bone defects often have weak cell vitality and differentiation ability of endogenous bone marrow mesenchymal stem cells (BMSCs), which makes bone regeneration face challenges. At present, the bone tissue engineering strategies are mainly to build grafts by loading cells on scaffolds in vitro. These strategies face many difficulties that limit their clinical application. To this end, we developed a new strategy for bone defect repair, namely chemotactic cell-free scaffolds combined with BMSCs injection. We first prepared a polydopamine-functionalized acellular fish scale scaffold that can continuously release stromal cell-derived factor 1α (SDF-1α) (termed as SDF-1α/PAFS) in vivo for at least 10 days. The study results showed that the scaffold not only has excellent mechanical properties and good biocompatibility but also has reactive oxygen scavenging activity, immunomodulation, angiogenesis, and osteogenesis. More importantly, SDF-1α/PAFS can recruit postoperatively injected BMSCs into bone defects for bone repair. We constructed the mouse cranial bone defect model, and in vivo experimental results confirmed that the strategy of combining SDF-1α/PAFS with BMSCs injection can effectively promote bone defect repair. Overall, this study provides a promising strategy for bone defect repair, with better clinical convenience and operability.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101759"},"PeriodicalIF":8.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DON-Apt19S bioactive scaffold transplantation promotes in situ spinal cord repair in rats with transected spinal cord injury by effectively recruiting endogenous neural stem cells and mesenchymal stem cells","authors":"Bi-Qin Lai ,&nbsp;Rong-Jie Wu ,&nbsp;Chuang-Ran Wu ,&nbsp;Hai-Yang Yu ,&nbsp;Jing Xu ,&nbsp;Shang-Bin Yang ,&nbsp;Zheng-Hong Chen ,&nbsp;Xing Li ,&nbsp;Yi-Nan Guo ,&nbsp;Yue Yang ,&nbsp;Ming-Tian Che ,&nbsp;Ting-Ting Wu ,&nbsp;Guang-Tao Fu ,&nbsp;Yu-Hui Yang ,&nbsp;Zhen Chen ,&nbsp;Nan Hua ,&nbsp;Rui Liu ,&nbsp;Qiu-Jian Zheng ,&nbsp;Yuan-Feng Chen","doi":"10.1016/j.mtbio.2025.101753","DOIUrl":"10.1016/j.mtbio.2025.101753","url":null,"abstract":"<div><div>The spinal cord's limited regeneration is attributed to the scarcity of endogenous stem cells and a poor post-injury microenvironment in adult mammals. To overcome these challenges, we transplanted a DNA aptamer 19S (Apt19S) sustained-release decellularized optic nerve (DON) scaffold (DON-A) into completely transected spinal cord injury (SCI) site in rats and investigated its effect on endogenous stem cell recruitment and differentiation, which subsequently contributed to <em>in situ</em> SCI repair. It has been demonstrated that Apt19S specifically binds to the membrane receptor alkaline phosphatase highly expressed on neural stem cells (NSCs) and mesenchymal stem cells (MSCs), and our study further proved that Apt19S can simultaneously recruit endogenous NSCs and MSCs to the lesion of SCI. In our study, the DON-A promoted stem cell proliferation in the early stage of the injury, followed by the rapid neurogenesis through NSCs and revascularization via MSCs. Synaptic connections between corticospinal tracts and calcitonin gene-related peptide positive nerve fibers with newborn neurons confirmed the formation of endogenous neuronal relays at the injury site, which improved the rats' motor and sensory functions. This study offers a new strategy for recruiting both NSCs and MSCs to synergistically overcome low spinal cord self-repair ability, holding a high potential for clinical translation.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101753"},"PeriodicalIF":8.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity","authors":"Yurong Xiang ,&nbsp;Qiang Liu ,&nbsp;Kang Liu ,&nbsp;Liuxian Chen ,&nbsp;Fengjiao Chen ,&nbsp;Tao Li ,&nbsp;Siqi Li ,&nbsp;Qiang Yu ,&nbsp;Quan Lv ,&nbsp;Zheng Xiang","doi":"10.1016/j.mtbio.2025.101732","DOIUrl":"10.1016/j.mtbio.2025.101732","url":null,"abstract":"<div><div>Multi-drug combination therapy is one of the most effective strategies for the treatment of drug-resistant and advanced tumors. Modern nanodrug delivery systems are crucial for multi-drug combination therapy and gene therapy. However, research on direct injection of RNAi has not yielded significant results. Artificial vectors are emerging as promising delivery systemts for RNA for gene therapy. In this study, a multi-drug therapy system was built based on a biodegradable exosome nano-platform exploiting the protective and low immunogenic properties of exosomes for RNA. This work aimed to accomplish the co-delivery of siRNA and 3-Bromopyruvic acid (3BP) on an exosome nanoplatform, enhancing targeting by coupling cetuximab (CTX) to exosome membranes, resulting in a new nanomedicine Exo@siRNA/3BP-CTX (ERBC) engineered exosomes. The synthesis conditions were optimized to obtain stable, safe, and effective nanomedicines. Successful targeting of tumors with CTX inhibited KRAS oncogene expression and significantly reduced glucose uptake by cancer cells. This enhanced the starvation therapy effect of the energy deprivation agent 3BP, thus promoting excessive autophagy activation in cells and doubling apoptosis. However, ERBC combined with CTX therapy restored cellular chemosensitivity to CTX. These findings indicate that engineered exosomes with dual therapeutic activities is a promising approach for treating refractory KRAS-mutant cancers.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101732"},"PeriodicalIF":8.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration","authors":"Kai Liu ,&nbsp;Jiangbo Shao ,&nbsp;Beibei Han ,&nbsp;Jianfeng Liu ,&nbsp;Shuai Yan ,&nbsp;Bin Liu ,&nbsp;Yao Liu","doi":"10.1016/j.mtbio.2025.101755","DOIUrl":"10.1016/j.mtbio.2025.101755","url":null,"abstract":"<div><div>Peripheral nerve regeneration (PNR) represents a substantial challenge in the medical field, primarily due to the limited regenerative capacity of the peripheral nerve system (PNS). Current research efforts are focused on developing advanced medical polymer materials to enhance nerve recovery. Despite significant progress, several critical issues remain unresolved, including biocompatibility, stability, mechanical strength, controlled degradation rates, and sustained release of therapeutic agents. This study examines the utilization of hyaluronic acid hydrogels, doped with mecobalamin (MeCbl) and conductive poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), in combination with exogenous electrical stimulation (ES) for PNR of rats. The strategy utilizes the MeCbl hydrogel to create a regenerative microenvironment and provide nutritional support for nerve cells, while PEDOT:PSS facilitates enhanced electrical signal conduction. ES has been shown to promote PNR and functional recovery, thereby demonstrating considerable potential. This study aims to comprehensively analyze the synergistic effects and potential value of this combined therapeutic approach, providing novel insights and pathways for the effective PNR.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101755"},"PeriodicalIF":8.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An NIR-responsive “4A hydrogel” encapsulating wormwood essential oil: through antibacterial, antioxidant, anti-inflammation, and angiogenic to promote diabetic wound healing","authors":"Mengjuan Tao ,&nbsp;Zhiwei Sun ,&nbsp;Haiyan Wang ,&nbsp;Na Meng ,&nbsp;Xiangru Chen ,&nbsp;Jianwei Mao ,&nbsp;Heyan Huang ,&nbsp;Yan Huang ,&nbsp;Jin Liu ,&nbsp;Zhenxing Wang ,&nbsp;Weiqiang Tan ,&nbsp;Yonggang Chen ,&nbsp;Chuchao Zhou ,&nbsp;Yanqing Yang","doi":"10.1016/j.mtbio.2025.101751","DOIUrl":"10.1016/j.mtbio.2025.101751","url":null,"abstract":"<div><div>The incorporation of hydrogels with biocompatible functional components to develop wound dressings exhibiting potent antibacterial, antioxidant, anti-inflammatory, and angiogenic properties to promote diabetic wound healing is highly desirable yet continues to pose a significant challenge. In this study, wormwood essential oil (WEO) is successfully encapsulated within black phosphorus (BP) using a physical extrusion technique. Subsequently, this composite is encapsulated within biocompatible gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) hydrogels to create multifunctional hydrogel dressing (WEO@BP/GH). In comparison to traditional hydrogels, BP enhances the encapsulation stability of WEO and improves the microenvironmental regulation capabilities through NIR-triggered release of WEO. Systemic in vitro experiments demonstrate that synergistic interaction between the diverse bioactive components of WEO and photothermal effects of BP results in highly effective antibacterial activities against <em>S. aureus</em> and <em>E. coli</em>, antioxidant of scavenging ROS, anti-inflammation of downregulating M1/M2 macrophages ratio, and angiogenic properties. Moreover, the in vivo tests demonstrate that WEO@BP/GH hydrogel significantly enhances high-performance diabetic wound repair through the acceleration of hemostasis, promotion of collagen deposition, regulation of inflammatory responses, and facilitation of vascularization. The findings indicate that WEO@BP/GH hydrogel holds considerable promise as a candidate for microenvironment regulation and effective diabetic wound healing across various clinical applications.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101751"},"PeriodicalIF":8.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"K+-H+ coupling strategy for immune regulation and bone defect repair","authors":"Lintao Hu ,&nbsp;Ke Yang ,&nbsp;Yiyu Chen ,&nbsp;Haoli Wang ,&nbsp;Zezhou Fu ,&nbsp;Lejian Jiang ,&nbsp;Jiachen Xu ,&nbsp;Hongsen Tian ,&nbsp;Yiwei Zhu ,&nbsp;Zhanqiu Dai ,&nbsp;Yijun Li ,&nbsp;Xianhua Chen ,&nbsp;Xianfeng Lin ,&nbsp;Pengfei Chen ,&nbsp;Chenhui Gu ,&nbsp;Shunwu Fan","doi":"10.1016/j.mtbio.2025.101744","DOIUrl":"10.1016/j.mtbio.2025.101744","url":null,"abstract":"<div><div>Ion homeostasis is crucial for maintaining cell function. Potassium ion (K⁺) is one of the most important cations in the human body, and it plays key role in maintaining biological activities and cellular functions, including the intricate balance of ion homeostasis that underpins both physiological and pathological processes. This study explored a novel role of K⁺ ions in regulating immune cell function and promoting tissue repair, especially in macrophage-mediated environments after severe tissue injury. We designed and synthesized a platelet-liposome vesicles loaded KHCO₃ (KHCO₃@PLV) that precisely delivered potassium bicarbonate to the site of injury extracellular after intravenous injection; then, precise ultrasound-triggered K⁺ release regulated extracellular K⁺ concentrations in the local macrophage environment. These effects collectively validate the K⁺-H⁺ coupling strategy - a novel mechanism whereby extracellular K⁺ elevation induces intracellular pH modulation, subsequently activating the AMPK/Nrf2 axis to reprogram macrophage metabolism and facilitating tissue regeneration through resolution of chronic inflammation. The main conclusion of the study is that an elevated extracellular K⁺ environment, which is an innovative treatment, is a potentially effective strategy for regulating immune responses and promoting repair after severe tissue injury.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101744"},"PeriodicalIF":8.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel therapeutic approach to hemangiomas: Combining photothermal therapy and ferroptosis in a microneedle delivery system","authors":"Yiming Geng ,&nbsp;Cancan Meng ,&nbsp;Hanzhi Zhang ,&nbsp;Chuan Ma ,&nbsp;Xiao Fu ,&nbsp;Haiwei Wu","doi":"10.1016/j.mtbio.2025.101748","DOIUrl":"10.1016/j.mtbio.2025.101748","url":null,"abstract":"<div><div>Infantile Hemangioma (IH) is the most common benign vascular tumor occurred in infants and young children. The larger hemangiomas or lesions located in specific areas can cause severe complications, such as disfigurement, obstruction, or ulceration, increasing the risk of functional impairment. Propranolol, serving as the first‐line drug for IH treatment, still poses various challenges. Certain patients exhibit low sensitivity to propranolol therapy or face recurrence, which become the leading reason for the failure of IH treatment. Additionally, the requirement for frequent daily medication can also complicate adherence for patients. Hence, developing novel IH therapy methods or drug administration routes is significantly important to enhance therapeutic effect and reduce side effects. Accordingly, in this study, we introduced an innovative photothermal, dissolving microneedles (MNs) patch designed specifically for IH therapy. Firstly, a pH responsive self-assembly nanoplatform with photothermal effect is designed by encapsulating propranolol (PRN) into zeolitic imidazolate framework-8 (ZIF-8) NPs and modifying with TA/Fe nanocomplexes. The fabricated PRN@ZIF-8@TA/Fe (PZ@TA/Fe) NPs exhibited good biocompatibility, pH-responsive degradation, photothermal conversion efficiency inside hemangioma endothelial cells (HemECs). Importantly, TA/Fe surface modification led to intracellular iron overload, which subsequently induced the Fenton reaction and triggered ferroptosis process. The combination of photothermal therapy and ferroptosis therapy exhibited a superior synergistic effect in damaging HemECs and vascular structures. The PZ@TA/Fe NPs loaded in MNs patch further ensured targeted delivery to lesion areas and achieved precise and maximal release of the PZ@TA/Fe NPs, while reducing systemic side effects to normal vasculature or tissue. The PZ@TA/Fe@MNs showed remarkable anti-angiogenic effect against IH in mice model. This study first investigates the potential of ferroptosis therapy in IH treatment, and highlights the substantial therapeutic effect of combining photothermal therapy and ferroptosis effects against IH proliferation. This approach can also become a more effective and safer treatment method for other diseases characterized by abnormal angiogenesis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101748"},"PeriodicalIF":8.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH/Hyal-responsive vancomycin-loaded chitooligosaccharide nanoparticles for intracellular MRSA infection treatment","authors":"Wenting Li ,&nbsp;WeiWei Li ,&nbsp;Xuanxiang Zhai,&nbsp;Xiao Liu,&nbsp;Xiaoyi Shi,&nbsp;Xiangjun Chen,&nbsp;Wei Hong","doi":"10.1016/j.mtbio.2025.101731","DOIUrl":"10.1016/j.mtbio.2025.101731","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>) is recognized as among the most critical bacterial pathogens globally. A significant portion of the complications associated with <em>S. aureus</em> infections arises from its ability to persist inside host phagocytes, particularly macrophages, making the eradication of intracellular <em>S. aureus</em> vital for therapeutic success. Regrettably, many antibiotics exhibit limited penetration into cells, underscoring the necessity for efficient intracellular delivery mechanisms. In this study, vancomycin-loaded chitooligosaccharide nanoparticles (COS@Van) coated with hyaluronic acid (HA), were engineered to function as an active-targeting antibiotic carrier recorded as HA/COS@Van. The HA coating serves as an external shell, which 1) covers the positive surface charge of COS NPs, thereby enhancing their biocompatibility and extending circulation time, and 2) facilitates targeted delivery to macrophages through specific interactions with the CD44 receptor. Confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) experiments confirmed that HA/COS could effectively accumulate in methicillin-resistant <em>S. aureus</em> (MRSA) infected macrophages. Additionally, when administered intravenously in mouse models, HA/COS demonstrated markedly increased accumulation in the liver, the primary location of infected macrophages. These findings highlight the active-targeting capabilities of HA/COS both <em>in vitro</em> and <em>in vivo</em> settings. Consequently, after being loaded with Van, HA/COS@Van exhibited superior efficacy in killing intracellular MRSA <em>in vitro</em>, as compared to free Van. Furthermore, HA/COS@Van also demonstrated enhanced bactericidal activity in both mouse acute peritonitis model and mouse organ infection model. Therefore, this active-targeting delivery system may hold promise in advancing therapeutic outcomes for infections related to intracellular pathogens.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101731"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bi-phasic integrated silk fibroin/polycaprolactone scaffolds for osteochondral regeneration inspired by the native joint tissue and interface","authors":"Zexing Zhang ,&nbsp;Qingquan Dong ,&nbsp;Zubing Li ,&nbsp;Gu Cheng ,&nbsp;Zhi Li","doi":"10.1016/j.mtbio.2025.101737","DOIUrl":"10.1016/j.mtbio.2025.101737","url":null,"abstract":"<div><div>Osteochondral scaffolds designed with bi-phasic and multi-phasic have typically struggled with post-implantation delamination. To address this issue, we developed a novel integrated scaffold with natural and continuous interface and heterogeneous bilayer structure. Through layer-by-layer wet electrospinning, two-dimensional (2D) bi-layer integrated membranes of silk fibroin (SF) and polycaprolactone (PCL) were fabricated. These membranes were then transformed into three-dimensional (3D) scaffolds using a CO₂ gas foaming technique, followed by gelatin coating on the osteogenic layer to afford final bi-phasic porous scaffolds. <em>In vitro</em> studies indicated that the 3D scaffolds better-maintained cell phenotypes than conventional 2D electrospun films. Additionally, the 3D scaffolds showed superior cartilage repair and osteoinductivity potential, with increased subchondral bone volume and reduced defect area in rat osteochondral defects models at 12 weeks. Taken together, these gas-foamed scaffolds were a promising candidate for osteochondral regeneration.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101737"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted biomimetic MOF-functionalized hydrogel scaffolds for bone regeneration: Synergistic osteogenesis and osteoimmunomodulation","authors":"San-yang Yu ,&nbsp;Ting Wu ,&nbsp;Kai-hao Xu ,&nbsp;Ru-yue Liu ,&nbsp;Tian-hao Yu ,&nbsp;Zhen-hua Wang ,&nbsp;Zhong-ti Zhang","doi":"10.1016/j.mtbio.2025.101740","DOIUrl":"10.1016/j.mtbio.2025.101740","url":null,"abstract":"<div><div>Critical-size bone defects remain a significant clinical challenge. The lack of endogenous stem cells with osteogenic differentiation potential in the defect area, combined with the inflammatory responses induced by scaffold implantation, highlights the need for biomaterials that can deliver stem cells and possess inflammatory regulation properties. In this study, we developed a 3D bioprinted gelatin methacrylate (GelMA) hydrogel scaffold modified with luteolin-loaded ZIF-8 (LUT@ZIF-8) nanoparticles, designed to deliver bone marrow mesenchymal stem cells (BMSCs) to the defect site and release bioactive components that promote osteogenesis and modulate the immune microenvironment. The LUT@ZIF-8/GelMA hydrogel scaffolds demonstrated excellent physical properties and biocompatibility. The sustained release of luteolin and zinc ions from the LUT@ZIF-8 nanoparticles conferred antibacterial, osteoinductive, and inflammatory regulation effects. The immune microenvironment modulated by LUT@ZIF-8/GelMA hydrogel scaffolds facilitated osteogenic differentiation of BMSCs. Furthermore, <em>in vivo</em> experiments confirmed the osteogenic and inflammatory regulation capabilities of the LUT@ZIF-8/GelMA hydrogel scaffolds. In conclusion, the 3D bioprinted LUT@ZIF-8/GelMA hydrogel scaffolds exhibit osteoimmunomodulatory properties, presenting a promising strategy for the treatment of bone defects.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"32 ","pages":"Article 101740"},"PeriodicalIF":8.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}