Junlin Lv , Meng Li , Xiaomin Wang , Liang Zhang , Dan Han , Xiaodan Hao , Xiao Xu , Yiwei Xu , Yan Tang , Zhen Shang , Nailong Pan , Xiaoying Kong , Wenhua Xu
{"title":"精确调节干细胞生存能力和环境的3d打印水凝胶用于糖尿病皮肤伤口愈合","authors":"Junlin Lv , Meng Li , Xiaomin Wang , Liang Zhang , Dan Han , Xiaodan Hao , Xiao Xu , Yiwei Xu , Yan Tang , Zhen Shang , Nailong Pan , Xiaoying Kong , Wenhua Xu","doi":"10.1016/j.mtbio.2025.102366","DOIUrl":null,"url":null,"abstract":"<div><div>Diabetic wounds not only suffer from vascular and nerve damage, but also face the severe challenge of impaired stem cell activity. In recent years, although traditional tissue engineering strategies provide exogenous stem cells for the healing of diabetic wounds, they have not reversed the dilemma of stem cell proliferation and differentiation in a high-glucose environment. In this work, piRNA-hsa-32182 was first demonstrated to be highly expressed in diabetic wounds and significantly inhibit the differentiation and migration of adipose-derived mesenchymal stem cells (ADMSCs). Accordingly, a 4D-printed tissue engineering hydrogel and piRNA-hsa-32182 antagomir were prepared to precisely modulate the survival microenvironment of ADMSCs and accelerate diabetic wound healing. 4D printed tissue engineering hydrogels provided a highly ordered microenvironment for ADMSCs through internal space homogenization, thereby effectively improving the loading rate and survival rate of stem cells. In addition, piRNA-hsa-32182 antagomir effectively enhanced the migration of ADMSCs, thereby increasing the deposition and maturation of collagen on the wound and promoting angiogenesis. In summary, this study significantly improved the microenvironment of wounds through the synergy of bio-intelligent printing technology and gene expression regulation, providing a new clinical paradigm for the treatment of diabetic wounds.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"35 ","pages":"Article 102366"},"PeriodicalIF":10.2000,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"4D-printed hydrogel with precise regulation of viability and environment of stem cells for diabetic skin wound healing\",\"authors\":\"Junlin Lv , Meng Li , Xiaomin Wang , Liang Zhang , Dan Han , Xiaodan Hao , Xiao Xu , Yiwei Xu , Yan Tang , Zhen Shang , Nailong Pan , Xiaoying Kong , Wenhua Xu\",\"doi\":\"10.1016/j.mtbio.2025.102366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Diabetic wounds not only suffer from vascular and nerve damage, but also face the severe challenge of impaired stem cell activity. In recent years, although traditional tissue engineering strategies provide exogenous stem cells for the healing of diabetic wounds, they have not reversed the dilemma of stem cell proliferation and differentiation in a high-glucose environment. In this work, piRNA-hsa-32182 was first demonstrated to be highly expressed in diabetic wounds and significantly inhibit the differentiation and migration of adipose-derived mesenchymal stem cells (ADMSCs). Accordingly, a 4D-printed tissue engineering hydrogel and piRNA-hsa-32182 antagomir were prepared to precisely modulate the survival microenvironment of ADMSCs and accelerate diabetic wound healing. 4D printed tissue engineering hydrogels provided a highly ordered microenvironment for ADMSCs through internal space homogenization, thereby effectively improving the loading rate and survival rate of stem cells. In addition, piRNA-hsa-32182 antagomir effectively enhanced the migration of ADMSCs, thereby increasing the deposition and maturation of collagen on the wound and promoting angiogenesis. In summary, this study significantly improved the microenvironment of wounds through the synergy of bio-intelligent printing technology and gene expression regulation, providing a new clinical paradigm for the treatment of diabetic wounds.</div></div>\",\"PeriodicalId\":18310,\"journal\":{\"name\":\"Materials Today Bio\",\"volume\":\"35 \",\"pages\":\"Article 102366\"},\"PeriodicalIF\":10.2000,\"publicationDate\":\"2025-09-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materials Today Bio\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590006425009378\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Today Bio","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590006425009378","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
4D-printed hydrogel with precise regulation of viability and environment of stem cells for diabetic skin wound healing
Diabetic wounds not only suffer from vascular and nerve damage, but also face the severe challenge of impaired stem cell activity. In recent years, although traditional tissue engineering strategies provide exogenous stem cells for the healing of diabetic wounds, they have not reversed the dilemma of stem cell proliferation and differentiation in a high-glucose environment. In this work, piRNA-hsa-32182 was first demonstrated to be highly expressed in diabetic wounds and significantly inhibit the differentiation and migration of adipose-derived mesenchymal stem cells (ADMSCs). Accordingly, a 4D-printed tissue engineering hydrogel and piRNA-hsa-32182 antagomir were prepared to precisely modulate the survival microenvironment of ADMSCs and accelerate diabetic wound healing. 4D printed tissue engineering hydrogels provided a highly ordered microenvironment for ADMSCs through internal space homogenization, thereby effectively improving the loading rate and survival rate of stem cells. In addition, piRNA-hsa-32182 antagomir effectively enhanced the migration of ADMSCs, thereby increasing the deposition and maturation of collagen on the wound and promoting angiogenesis. In summary, this study significantly improved the microenvironment of wounds through the synergy of bio-intelligent printing technology and gene expression regulation, providing a new clinical paradigm for the treatment of diabetic wounds.
期刊介绍:
Materials Today Bio is a multidisciplinary journal that specializes in the intersection between biology and materials science, chemistry, physics, engineering, and medicine. It covers various aspects such as the design and assembly of new structures, their interaction with biological systems, functionalization, bioimaging, therapies, and diagnostics in healthcare. The journal aims to showcase the most significant advancements and discoveries in this field. As part of the Materials Today family, Materials Today Bio provides rigorous peer review, quick decision-making, and high visibility for authors. It is indexed in Scopus, PubMed Central, Emerging Sources, Citation Index (ESCI), and Directory of Open Access Journals (DOAJ).