精确调节干细胞生存能力和环境的3d打印水凝胶用于糖尿病皮肤伤口愈合

IF 10.2 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Junlin Lv , Meng Li , Xiaomin Wang , Liang Zhang , Dan Han , Xiaodan Hao , Xiao Xu , Yiwei Xu , Yan Tang , Zhen Shang , Nailong Pan , Xiaoying Kong , Wenhua Xu
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引用次数: 0

摘要

糖尿病创面不仅存在血管和神经损伤,还面临着干细胞活性受损的严峻挑战。近年来,虽然传统的组织工程策略为糖尿病伤口的愈合提供了外源干细胞,但并没有扭转高糖环境下干细胞增殖和分化的困境。在这项工作中,piRNA-hsa-32182首次被证明在糖尿病伤口中高表达,并显著抑制脂肪源性间充质干细胞(ADMSCs)的分化和迁移。为此,我们制备了3d打印的组织工程水凝胶和piRNA-hsa-32182安塔戈米尔,以精确调节ADMSCs的生存微环境,加速糖尿病伤口愈合。4D打印组织工程水凝胶通过内部空间均质为ADMSCs提供了高度有序的微环境,从而有效提高了干细胞的加载率和存活率。piRNA-hsa-32182 antagomir有效增强ADMSCs的迁移,从而增加胶原在创面上的沉积和成熟,促进血管生成。综上所述,本研究通过生物智能打印技术与基因表达调控的协同作用,显著改善了创面微环境,为糖尿病创面治疗提供了新的临床范例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
4D-printed hydrogel with precise regulation of viability and environment of stem cells for diabetic skin wound healing
Diabetic wounds not only suffer from vascular and nerve damage, but also face the severe challenge of impaired stem cell activity. In recent years, although traditional tissue engineering strategies provide exogenous stem cells for the healing of diabetic wounds, they have not reversed the dilemma of stem cell proliferation and differentiation in a high-glucose environment. In this work, piRNA-hsa-32182 was first demonstrated to be highly expressed in diabetic wounds and significantly inhibit the differentiation and migration of adipose-derived mesenchymal stem cells (ADMSCs). Accordingly, a 4D-printed tissue engineering hydrogel and piRNA-hsa-32182 antagomir were prepared to precisely modulate the survival microenvironment of ADMSCs and accelerate diabetic wound healing. 4D printed tissue engineering hydrogels provided a highly ordered microenvironment for ADMSCs through internal space homogenization, thereby effectively improving the loading rate and survival rate of stem cells. In addition, piRNA-hsa-32182 antagomir effectively enhanced the migration of ADMSCs, thereby increasing the deposition and maturation of collagen on the wound and promoting angiogenesis. In summary, this study significantly improved the microenvironment of wounds through the synergy of bio-intelligent printing technology and gene expression regulation, providing a new clinical paradigm for the treatment of diabetic wounds.
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来源期刊
CiteScore
8.30
自引率
4.90%
发文量
303
审稿时长
30 days
期刊介绍: Materials Today Bio is a multidisciplinary journal that specializes in the intersection between biology and materials science, chemistry, physics, engineering, and medicine. It covers various aspects such as the design and assembly of new structures, their interaction with biological systems, functionalization, bioimaging, therapies, and diagnostics in healthcare. The journal aims to showcase the most significant advancements and discoveries in this field. As part of the Materials Today family, Materials Today Bio provides rigorous peer review, quick decision-making, and high visibility for authors. It is indexed in Scopus, PubMed Central, Emerging Sources, Citation Index (ESCI), and Directory of Open Access Journals (DOAJ).
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