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The Correlation between Serum Cortisol Concentration in Mammals and the Level of Global Seismic Activity. 哺乳动物血清皮质醇浓度与全球地震活动水平之间的相关性。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06256-z
M E Diatroptov
{"title":"The Correlation between Serum Cortisol Concentration in Mammals and the Level of Global Seismic Activity.","authors":"M E Diatroptov","doi":"10.1007/s10517-024-06256-z","DOIUrl":"10.1007/s10517-024-06256-z","url":null,"abstract":"<p><p>A positive correlation was found between the level of global seismic activity and dynamics of cortisol concentration in blood serum of male rabbits (r=0.33, p=0.01) and Campbell's dwarf hamsters (r=0.38, p=0.04). For a small group of healthy volunteers (n=5), we also found a positive correlation between cortisol levels and the number of major earthquakes, excluding aftershocks (r=0.36, p=0.009) and a negative correlation with IL-18 levels (r=-0.28, p=0.04). The body response to earthquakes is not delayed, and the numerous aftershocks do not seem to affect the biological indicators under study. These facts showed that the earthquakes themselves do not affect the cortisol level via the changes of the environmental parameters. In contrast, the underlying biotropic factor is an unknown trigger responsible for an increase in the number of potent earthquakes. Evidently, it is important to take into account the level of global seismicity as a marker of this trigger provoking the rise of glucocorticoid hormones.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"716-720"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant Effects of Tryptanthrin Oxime. 胰黄素肟的抗氧化作用
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06261-2
M B Plotnikov, G A Chernysheva, V I Smol'yakova, O I Aliev, A R Kovrizhina, A I Khlebnikov, A G Drozd, E V Plotnikov
{"title":"Antioxidant Effects of Tryptanthrin Oxime.","authors":"M B Plotnikov, G A Chernysheva, V I Smol'yakova, O I Aliev, A R Kovrizhina, A I Khlebnikov, A G Drozd, E V Plotnikov","doi":"10.1007/s10517-024-06261-2","DOIUrl":"10.1007/s10517-024-06261-2","url":null,"abstract":"<p><p>We studied the radical-binding and antioxidant activities of the alkaloid tryptanthrin (TR) and its new synthetic derivative tryptanthrin oxime (TR-Ox), as well as the cytoprotective activity of TR-Ox under conditions of oxidative stress. The antiradical activity of TR-Ox was revealed in the test of binding with stable chromogen radical 2,2-diphenyl-1-picrylhydrazyl and in the superoxide radical generation test (riboflavin photoreduction reaction with detection by NBT reduction). TR-Ox was inferior to ionol and dihydroquercetin by the antiradical activity. In these tests, TR did not exhibit antiradical activity. TR-Ox did not show iron-chelating activity (in the test with the formation of the o-phenanthroline-Fe<sup>2+</sup> complex and its destruction in the presence of chelating agents). In brain homogenate, TR-Ox significantly reduced the increase in spontaneous chemiluminescence. Under conditions of oxidative stress induced by 15 mM H<sub>2</sub>O<sub>2</sub> in the SH-SY5Y neuroblastoma cell culture, TR-Ox exhibited cytoprotective activity and increased the number of viable cells.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"741-744"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction of Disorders in Psychoneurological Status and Functioning of Progenitor Cells of the Nervous Tissue with NF-κB Inhibitor under Conditions of Alzheimer's Disease Modeling. 在阿尔茨海默氏症模型条件下用 NF-κB 抑制剂纠正神经组织祖细胞的精神神经状态和功能紊乱
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06258-x
G N Zyuz'kov, L A Miroshnichenko, T Yu Polyakova, E V Simanina, A V Chaykovskyi, V I Agafonov, V V Zhdanov
{"title":"Correction of Disorders in Psychoneurological Status and Functioning of Progenitor Cells of the Nervous Tissue with NF-κB Inhibitor under Conditions of Alzheimer's Disease Modeling.","authors":"G N Zyuz'kov, L A Miroshnichenko, T Yu Polyakova, E V Simanina, A V Chaykovskyi, V I Agafonov, V V Zhdanov","doi":"10.1007/s10517-024-06258-x","DOIUrl":"10.1007/s10517-024-06258-x","url":null,"abstract":"<p><p>We studied the effect of NF-κB blockade on the state of various pools of progenitor cells of the nervous tissue and the psychoneurological status of experimental animals with modeled Alzheimer's disease. Administration of scopolamine hydrobromide to C57BL/6 mice for 4 weeks was accompanied by the development of \"persistent\" disturbances in the orientation and exploratory behavior and mnestic function. An ameliorating effect of the NF-κB inhibitor on these cognitive disorders typical of senile dementia was revealed. At the same time, we observed an increase in the content of neural stem cells and committed neuronal precursors in the subventricular zone of the brain.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"725-730"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Bacterial Nanocellulose Consumption on the Content of Macronutrients and Trace Elements in the Organs of Rats. 食用细菌纳米纤维素对大鼠器官中宏量营养素和微量元素含量的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI: 10.1007/s10517-024-06262-1
A I Kolobanov, A A Shumakova, V A Shipelin, I E Sokolov, K Z Maisaya, I V Gmoshinski, S A Khotimchenko
{"title":"Influence of Bacterial Nanocellulose Consumption on the Content of Macronutrients and Trace Elements in the Organs of Rats.","authors":"A I Kolobanov, A A Shumakova, V A Shipelin, I E Sokolov, K Z Maisaya, I V Gmoshinski, S A Khotimchenko","doi":"10.1007/s10517-024-06262-1","DOIUrl":"10.1007/s10517-024-06262-1","url":null,"abstract":"<p><p>Bacterial nanocellulose (BNC) prepared by the methods of \"green\" bionanotechnological synthesis is considered a promising food additive and food ingredient. At the same time, the risk of reducing the bioavailability of minerals due to their adsorption on BNC fibers having a high specific surface area and high adsorption and ion exchange capacity cannot be excluded. We studied the effect of oral administration of BNC on the accumulation of macronutrients and trace elements included in the diet in the liver and kidneys of laboratory animals. Male Wistar rats received BNC at doses of 0 (control), 1, 10, and 100 mg/kg body weight as part of their diet for 8 weeks. The content of 30 macronutrients and trace elements in the liver and kidneys was determined by inductively coupled plasma mass spectrometry. It was found that BNC at all doses did not significantly change the content of the main essential macronutrients and trace elements in the organs (Ca, Cr, Cu, Fe, K, Mg, Mn, Na, P, Se, and Zn), which indicates the absence of a negative effect on their bioavailability. Among other elements, a statistically significant decrease in the content of As, B, Cd, Co, and Pb in the liver and an increase in Al, B, Ba, Ni, and Pb in the kidneys were revealed (more than 20% of the control). The revealed decrease in the bioaccumulation of cobalt can indicate inhibition of assimilation of certain chemical forms of this trace element under the action of BNC.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"745-750"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Study of Cytotoxicity and Accumulation of Boron and Lithium-Containing Drugs in Skin Melanoma Cells In Vitro. 含硼和含锂药物在皮肤黑色素瘤细胞体外细胞毒性和蓄积的比较研究
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06260-3
A I Kasatova, I A Razumov, S Yu Taskaev, Iu S Taskaeva
{"title":"Comparative Study of Cytotoxicity and Accumulation of Boron and Lithium-Containing Drugs in Skin Melanoma Cells In Vitro.","authors":"A I Kasatova, I A Razumov, S Yu Taskaev, Iu S Taskaeva","doi":"10.1007/s10517-024-06260-3","DOIUrl":"10.1007/s10517-024-06260-3","url":null,"abstract":"<p><p>We studied cytotoxicity and accumulation of boron and lithium by cultured human fibroblasts and human and mouse skin melanoma cell cultures. The cytotoxicity of boron and lithium drugs was assessed by MTT tests in the boron and lithium concentration range of 10-640 μg/ml. Cell viability was significantly reduced after incubation with boron and lithium at concentrations >160 μg/ml. To assess accumulation of boron and lithium, the concentration of elements was measured using inductively coupled plasma atomic emission spectrometry. Melanoma cells more intensively accumulated lithium in comparison with boron. The results indicate the possibility of safe application of lithium salts in concentrations minimally required for successful neutron capture therapy.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"736-740"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Sodium Aminophthalhydrazide on Structural and Functional Characteristics of Pancreatic Islands in Experimental Type 2 Diabetes Mellitus. 氨苯喋啶钠对实验性 2 型糖尿病胰岛结构和功能特征的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06257-y
A V Belousova, K V Sokolova, I G Danilova, V A Chereshnev, M T Abidov
{"title":"Effect of Sodium Aminophthalhydrazide on Structural and Functional Characteristics of Pancreatic Islands in Experimental Type 2 Diabetes Mellitus.","authors":"A V Belousova, K V Sokolova, I G Danilova, V A Chereshnev, M T Abidov","doi":"10.1007/s10517-024-06257-y","DOIUrl":"10.1007/s10517-024-06257-y","url":null,"abstract":"<p><p>Under the influence of inflammation, pancreatic β cells can transdifferentiate into cells with a different phenotype. When inflammation decreases, the opposite process is possible. We studied the effect of intramuscular injection of 5-amino-2,3-dihydrophthalazine-1,4-dione sodium salt (APH) on the structural and functional characteristics of the pancreatic islets in rats with experimental type 2 diabetes mellitus. Insulin-producing, glucagon-producing, and proliferating cells were identified by immunohistochemistry. After APH administration, an increase in the number of β cells, a decrease in the number of α cells and cells synthesizing both insulin and glucagon (insulin-glucagon-positive) were observed; mitotic activity of β cells did not change. It is likely that APH promotes transdifferentiation of α cells into β cells by changing the microenvironment of endocrine cells and reducing inflammation in pancreatic islets.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"721-724"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Nitric Oxide Supplied to the Sweep Gas of the Oxygenator on the Formation of Gaseous Microemboli during Cardiopulmonary Bypass (Experimental Study). 一氧化氮加入氧合器扫气对心肺旁路过程中气态微栓子形成的影响（实验研究）。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI: 10.1007/s10517-024-06268-9
A O Marichev, A M Radovskiy, V V Osovskikh, D A Kozyrev, I G Chomakhashvili, A A Lomaev, A K Sorokina, I A Chinenkov, O I Vasilchenko, D A Avezov, M O Kalinin, A E Bautin
{"title":"The Effect of Nitric Oxide Supplied to the Sweep Gas of the Oxygenator on the Formation of Gaseous Microemboli during Cardiopulmonary Bypass (Experimental Study).","authors":"A O Marichev, A M Radovskiy, V V Osovskikh, D A Kozyrev, I G Chomakhashvili, A A Lomaev, A K Sorokina, I A Chinenkov, O I Vasilchenko, D A Avezov, M O Kalinin, A E Bautin","doi":"10.1007/s10517-024-06268-9","DOIUrl":"10.1007/s10517-024-06268-9","url":null,"abstract":"<p><p>The effect of nitric oxide (NO) supplied to the sweep gas of the oxygenator on the formation of gaseous microemboli during cardiopulmonary bypass has been studied in animal experiments (female pigs). It was shown that NO added to the sweep gas of the oxygenator during cardiopulmonary bypass significantly decreased the number and volume of microemboli (the number of microemboli over 1 h of cardiopulmonary bypass was 1197 (568; 2436) vs 55,478 (15,217; 331,480) in the control; p=0.016). The decrease in the number and volume of all bubbles was accompanied by a significant decrease in the concentration of neuron-specific enolase, a marker of brain injury, 6 h after the end of cardiopulmonary bypass to 7.7 (7.5; 8.7) ng/ml vs 11.2 (9.2; 18.3) ng/ml in the group without NO (p=0.047).</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"787-791"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reactive Changes in Lymph Node Structure in Peritonitis and during Treatment with a New Antibiotic. 腹膜炎和新型抗生素治疗期间淋巴结结构的反应性变化
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI: 10.1007/s10517-024-06269-8
S N Abdreshov, G A Demchenko, V N Gorchakov, M A Yessenova, A N Yeshmukhanbet
{"title":"Reactive Changes in Lymph Node Structure in Peritonitis and during Treatment with a New Antibiotic.","authors":"S N Abdreshov, G A Demchenko, V N Gorchakov, M A Yessenova, A N Yeshmukhanbet","doi":"10.1007/s10517-024-06269-8","DOIUrl":"10.1007/s10517-024-06269-8","url":null,"abstract":"<p><p>The effect of a new antibiotic peptomide A-70 on changes in the mesenterial lymph nodes caused by experimental peritonitis was studied. Differences in the morphological changes in rat lymph nodes in peritonitis and against the background of antibiotic therapy were revealed. Lymph nodes responded to peritoneal inflammation by reducing the area of cortical structures and expansion of the sinus system, which indicates a decrease in drainage-detoxification and immune function and determines unfavorable outcome of peritonitis. Antibiotic therapy reduced inflammatory manifestations and toxic pressure on the lymph system and potentiated the reactive response of the mesenteric lymph nodes: the size of lymphoid nodes and the paracortical T-dependent zone increased against the background of shrinkage of the sinus system, which attested to activation of the lymphopoietic function and immune response of lymph nodes in peritonitis. The observed changes attested to favorable prognosis of peritonitis treated with antibiotic peptomide A-70.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"792-796"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer. STRAP 基因敲除抑制非小细胞肺癌的迁移和生长
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06267-w
X Chen, C Xu, Y Yang, L Li, R Hong
{"title":"STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer.","authors":"X Chen, C Xu, Y Yang, L Li, R Hong","doi":"10.1007/s10517-024-06267-w","DOIUrl":"10.1007/s10517-024-06267-w","url":null,"abstract":"<p><p>Serine-threonine kinase receptor-associated protein (STRAP) regulates cell proliferation and apoptosis by binding to many target proteins and plays an important regulatory role in tumor development. We studied the effects of STRAP on non-small cell lung cancer (NSCLC) in vivo and in vitro in order to elucidate possible mechanisms underlying the regulatory effects of this protein. The levels of STRAP in NSCLC tissues and cells were determined by quantitative reverse transcription PCR, immunohistochemical staining, and Western blotting. In in vitro experiments, A549 and HCC827 cells were transfected with small interfering RNA (siRNA) to knockdown STRAP (si-STRAP) or with negative control sequence; cell migration and invasion were detected by scratch and Transwell assays, respectively. The expression levels of X-linked inhibitor of apoptosis protein (XIAP), caspase-3, and caspase-9 were determined by Western blotting. In addition, we analyzed changes of tumor volume in a nude mouse NSCLC model. STRAP was highly expressed in NSCLC tissues and cells, but its expression was significantly suppressed in A549 and HCC827 cells transfected with si-STRAP. STRAP knockdown resulted in a significant inhibition of migration and invasion of A549 and HCC827 cells. It also significantly reduced the expression of XIAP and elevated expression of caspase-3 and caspase-9. In nude mice with tumor originated from transplanted A549 cells, inhibition of STRAP expression retarded the tumor growth. Overall, these findings indicate that STRAP is overexpressed in NSCLC, while knockdown of STRAP gene inhibits the growth of NSCLC.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"780-786"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective Effect of Protocatechuic Aldehyde on Cerebral Ischemia/Reperfusion Injury in Rats through Blood-Brain Barrier Protection. 原儿茶醛通过血脑屏障保护作用对大鼠脑缺血再灌注损伤的保护作用
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06264-z
F He, J Feng, H Sun, Y Xu, H Yan, X Song, Y Wang, X Li, Q Lin
{"title":"Protective Effect of Protocatechuic Aldehyde on Cerebral Ischemia/Reperfusion Injury in Rats through Blood-Brain Barrier Protection.","authors":"F He, J Feng, H Sun, Y Xu, H Yan, X Song, Y Wang, X Li, Q Lin","doi":"10.1007/s10517-024-06264-z","DOIUrl":"10.1007/s10517-024-06264-z","url":null,"abstract":"<p><p>Cerebral ischemia can lead to destruction of the blood-brain barrier (BBB), the main cause of cerebral edema and cerebral infarction. BBB damage is also one of the key factors affecting the result of drug therapy. We studied the protective effect of 5-day pretreatment with protocatechuic aldehyde (PAL) at doses of 10 and 20 mg/kg on BBB function and structure after middle cerebral artery occlusion/reperfusion (MCAO/R) in rats. The infarct volume, behavioral neurological deficit score, and Evans blue content in the brain were estimated. We also evaluated the content of nitric oxide (NO) and activities of inducible and neuronal NO synthases. Expression of aquaporin-4 (AQP-4), occludin, claudin-5, and MMP-3 in the brain tissues was estimated by Western blotting. The BBB ultrastructure was analyzed under an electron microscope. We revealed that PAL at both used doses significantly reduced the neurological deficit score, brain infarct volume, and Evans blue extravasation. Electron microscopy showed that PAL significantly improved the ultrastructure of BBB and alleviated its injury. Pretreatment with PAL increased expression of occludin and claudin-5 and reduced expression of AQP-4 and MMP-3. At the same time, the release of NO and activities of NO synthases were notably inhibited. Our results suggest that PAL can be a promising compound to attenuate cerebral ischemia resulting from occlusion/reperfusion injury via BBB protection.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"763-769"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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