发布求助
文献互助 智能选刊 最新文献

Bulletin of Experimental Biology and Medicine最新文献

筛选
英文 中文
Specific Matrix on the Basis of Angiotensin-Converting Enzyme 2: Development and Functional Analysis. 基于血管紧张素转换酶2特异性基质的研制及功能分析。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06463-2
N N Kostin, T V Bobik, N Yu Rushkevich, G A Skryabin, E N Kaliberda, V D Knorre, I V Smirnov, A G Gabibov
{"title":"Specific Matrix on the Basis of Angiotensin-Converting Enzyme 2: Development and Functional Analysis.","authors":"N N Kostin, T V Bobik, N Yu Rushkevich, G A Skryabin, E N Kaliberda, V D Knorre, I V Smirnov, A G Gabibov","doi":"10.1007/s10517-025-06463-2","DOIUrl":"10.1007/s10517-025-06463-2","url":null,"abstract":"<p><p>In this study, an affinity matrix based on recombinant human angiotensin-converting enzyme 2 (ACE2) immobilized on a porous polymeric substrate was developed and characterized. The matrix efficiently binds viral particles pseudotyped with the SARS-CoV-2 spike protein. These findings highlight the prospects for creating novel affinity matrices for extracorporeal hemoperfusion aimed at reducing viral load in patients with high viremia and severe COVID-19.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"228-231"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavioral Characteristics of Female Rats on the Model of Pain Syndrome in the Maxillofacial Region. 雌性大鼠颌面部疼痛综合征模型的行为特征。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06454-3
A V Klimenko, D A Cheremisova, O S Romanenko, S S Pertsov
{"title":"Behavioral Characteristics of Female Rats on the Model of Pain Syndrome in the Maxillofacial Region.","authors":"A V Klimenko, D A Cheremisova, O S Romanenko, S S Pertsov","doi":"10.1007/s10517-025-06454-3","DOIUrl":"10.1007/s10517-025-06454-3","url":null,"abstract":"<p><p>Changes in behavioral parameters in the open field and elevated plus maze tests were studied in female rats with experimental osteoarthritis of the temporomandibular joint (TMJ) caused by intra-articular injection of sodium monoiodoacetate (16 mg/kg). In the elevated plus maze, the animals with experimental pain syndrome demonstrated the following dynamics of behavioral parameters: the latency of leaving the central platform and the time spent in closed arms increased, while the number of visits to closed arms and numbers of head dips and rearings decreased on day 25 of the experiment in comparison with the baseline levels. In the open-field, the latency of the first movement and grooming time increased and the number of objects explored decreased by day 26. Changes in the behavior of rats with pain syndrome manifested in a pronounced inhibition of orientation and exploratory activity, increased anxiety, and suppression of motor activity and persisted for a long time after injection of sodium monoiodoacetate into TMJ.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"181-185"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characteristics of Sarcomere Proteins in Myocardial Sleeves of the Superior Vena Cava and Pulmonary Veins of the Porcine Heart. 猪心脏上腔静脉和肺静脉心肌套管中肌节蛋白的特征。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06452-5
A M Kochurova, E A Beldiia, Yu Ya Antonets, G V Kopylova, D V Shchepkin
{"title":"Characteristics of Sarcomere Proteins in Myocardial Sleeves of the Superior Vena Cava and Pulmonary Veins of the Porcine Heart.","authors":"A M Kochurova, E A Beldiia, Yu Ya Antonets, G V Kopylova, D V Shchepkin","doi":"10.1007/s10517-025-06452-5","DOIUrl":"10.1007/s10517-025-06452-5","url":null,"abstract":"<p><p>Myocardial sleeves around caval and pulmonary veins have ectopic activity, which is the main cause of atrial arrhythmias. The morphological and electrophysiological properties of this myocardium differ from those of the atria, but its mechanical activity in large animals has not been studied. We compared the phosphorylation of sarcomere proteins and the functional characteristics of myosin from the left and right atria, superior vena cava, and pulmonary veins of the porcine heart. Myosin from myocardial sleeves contains more of the β-isoform of the heavy chain, i.e., is characterized by slower kinetics of cross-bridge attachment/detachment than atrial myosin. In myocardial sleeves, phosphorylation of troponin T and I, and tropomyosin was lower than in the atria. The phosphorylation features of thin filament proteins may affect the mechanical characteristics of cardiomyocyte contraction, as well as the mechanisms, regulating myocardial contraction, such as the length-dependent regulation and the mechanoelectrical feedback.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"171-176"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online Solid-Phase Extraction. 高效液相色谱-串联质谱在线固相萃取法分析血清血清素。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06474-z
D V Bychenkov, V A Ioutsi, I V Kirilina, M V Ovcharov, M A Antsupova, A K Lubenskaya, L K Abdullaev, S A Pantsernaya, N G Mokrysheva
{"title":"Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online Solid-Phase Extraction.","authors":"D V Bychenkov, V A Ioutsi, I V Kirilina, M V Ovcharov, M A Antsupova, A K Lubenskaya, L K Abdullaev, S A Pantsernaya, N G Mokrysheva","doi":"10.1007/s10517-025-06474-z","DOIUrl":"10.1007/s10517-025-06474-z","url":null,"abstract":"<p><p>We propose a method for the quantitative determination of serotonin in blood serum using high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and online solid-phase extraction in a chromatographic system. This approach allows serotonin quantification in blood serum within a range of 0.5-2000 ng/ml, partially automates the sample preparation process, and minimizes the influence of matrix components. The method is designed for the quantitative measurement of serotonin levels in patients with various deviations of serotonin levels and associated diseases.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"282-286"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Immunogenicity of an Artificial Tick-Borne Encephalitis Virus Polyepitope Immunogen May Depend on Spacers Flanking Epitope. 一种人工蜱传脑炎病毒多肽类免疫原的免疫原性可能取决于表位两侧的间隔物。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06462-3
E V Tigeeva, E V Shaburova, D V Antonets, D N Kisakov, M B Borgoyakova, E V Starostina, V A Yakovlev, L A Kisakova, N B Rudometova, A P Rudometov, L I Karpenko
{"title":"The Immunogenicity of an Artificial Tick-Borne Encephalitis Virus Polyepitope Immunogen May Depend on Spacers Flanking Epitope.","authors":"E V Tigeeva, E V Shaburova, D V Antonets, D N Kisakov, M B Borgoyakova, E V Starostina, V A Yakovlev, L A Kisakova, N B Rudometova, A P Rudometov, L I Karpenko","doi":"10.1007/s10517-025-06462-3","DOIUrl":"10.1007/s10517-025-06462-3","url":null,"abstract":"<p><p>The development of artificial polyepitope immunogens based on the conserved CD4<sup>+</sup> and CD8<sup>+</sup> epitopes from different virus proteins combined into a single molecule is a promising approach in the development of vaccines for activating a specific T-cell response. Selection of spacer sequences flanking the epitopes within the construct and providing the optimal processing of such immunogens within the cell is an important step in the design of such vaccines. We report the results of designing polyepitope T-cell immunogens of tick-borne encephalitis virus that differ in the presence or absence of optimally selected alanine spacers between epitopes, as well as the data of comparative analysis of immunogenic properties of DNA vaccines encoding the obtained polyepitope immunogens.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"223-227"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sexual Behavior of the Offspring of Female Rats Exposed to Bisphenol A. 暴露于双酚A的雌性大鼠后代的性行为。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06423-w
G V Bryukhin, D S Laskov, N V Gribachyova, D R Solyannikova
{"title":"Sexual Behavior of the Offspring of Female Rats Exposed to Bisphenol A.","authors":"G V Bryukhin, D S Laskov, N V Gribachyova, D R Solyannikova","doi":"10.1007/s10517-025-06423-w","DOIUrl":"10.1007/s10517-025-06423-w","url":null,"abstract":"<p><p>We studied the effect of bisphenol A orally administrated to female rats on sexual behavior of their offspring. The males of the experimental group demonstrated significantly decreased number of approaches to a partition separating them from a receptive female; the copulatory rate during direct interaction with the female was also reduced. In females of the experimental group, the lordosis quotient and lordosis intensity were decreased. The revealed changes indicate that bisphenol A intoxication of female rats leads to significant disturbance of sexual behavior in their offspring of both sexes.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"10-13"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Attention Concentration in Sexually Mature Male Rhesus Monkeys (Macaca mulatta) and Hamadryas Baboons (Papio hamadryas). 性成熟雄性恒河猴（Macaca mulatta）和Hamadryas狒狒（Papio Hamadryas）注意力集中的评价。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-21 DOI: 10.1007/s10517-025-06443-6
A E Anikaev, N V Meishvili, V G Chalyan, E N Anikaeva
{"title":"Evaluation of Attention Concentration in Sexually Mature Male Rhesus Monkeys (Macaca mulatta) and Hamadryas Baboons (Papio hamadryas).","authors":"A E Anikaev, N V Meishvili, V G Chalyan, E N Anikaeva","doi":"10.1007/s10517-025-06443-6","DOIUrl":"10.1007/s10517-025-06443-6","url":null,"abstract":"<p><p>A comparative study of the level of concentration of attention and related indicators (reaction latency, level and dynamics of activity during task solving, and number of approaches) was conducted in mature male rhesus monkeys (Macaca mulatta) and hamadryas baboons (Papio hamadryas). It was found that all the studied indicators in baboons were higher than in macaques. At the same time, macaques and baboons showed similar dynamics of activity during the test.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"108-111"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of Periodontal Endogenous Regeneration by 3D Bioscaffolds. 3D生物支架诱导牙周内源性再生。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06442-7
A A Ivanov, O P Popova, A V Kuznetsova, N N Butorina, T I Danilova, A V Latyshev, O O Yanushevich
{"title":"Induction of Periodontal Endogenous Regeneration by 3D Bioscaffolds.","authors":"A A Ivanov, O P Popova, A V Kuznetsova, N N Butorina, T I Danilova, A V Latyshev, O O Yanushevich","doi":"10.1007/s10517-025-06442-7","DOIUrl":"10.1007/s10517-025-06442-7","url":null,"abstract":"<p><p>The potential of a combination of decellularized tooth matrices and decellularized periodontal ligament in a collagen I hydrogel for periodontal regeneration was studied in a rat model of surgical periodontal defect. The results were evaluated using microcomputed tomography and histological and immunohistochemical methods on day 60. The bioengineered construct induced the formation of dental cement and new fibrous and bone tissues in the area of the periodontal ligament. This bioengineered construct can be considered as a possible bioscaffold for the restoration of the periodontal tissue.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"103-107"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effectiveness of Recombinant Human Angiogenin during Skin Burn in CD-1 Mice. 重组人血管生成素在CD-1小鼠皮肤烧伤中的作用。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06437-4
A P Lykov, A M Goryachkin, A A Prutov, M N Drovosekov, O V Poveshchenko
{"title":"The Effectiveness of Recombinant Human Angiogenin during Skin Burn in CD-1 Mice.","authors":"A P Lykov, A M Goryachkin, A A Prutov, M N Drovosekov, O V Poveshchenko","doi":"10.1007/s10517-025-06437-4","DOIUrl":"10.1007/s10517-025-06437-4","url":null,"abstract":"<p><p>A comparative study was conducted to investigate the effects of recombinant human angiogenin and the conditioned media of human somatic cells (bone marrow mesenchymal stromal cells, EA.Hy926 endothelial cells, and human skin fibroblasts) on burn wound healing and the levels of IL-1β, TNFα, IL-10, VEGF, and NO in blood serum and skin samples from CD-1 mice. The study found that angiogenin, similar to the conditioned media of human somatic cells, accelerated wound healing in burns. The number of cytokines in the blood serum and skin changes when angiogenin and conditioned medium of human somatic cells are applied to the burn wound site. Recombinant human angiogenin demonstrated therapeutic potential comparable to that of conditioned media from human somatic cells in terms of wound healing.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"99-102"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Influence of Bisphenol A on the Molecular Biological Characteristics of Human Colon Adenocarcinoma HT-29 Culture Cells. 双酚A对人结肠腺癌HT-29培养细胞分子生物学特性的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06447-2
E A Miroshnichenko, M A Diatroptova, A I Alekseeva, A D Gerasimov, A M Kosyreva
{"title":"The Influence of Bisphenol A on the Molecular Biological Characteristics of Human Colon Adenocarcinoma HT-29 Culture Cells.","authors":"E A Miroshnichenko, M A Diatroptova, A I Alekseeva, A D Gerasimov, A M Kosyreva","doi":"10.1007/s10517-025-06447-2","DOIUrl":"10.1007/s10517-025-06447-2","url":null,"abstract":"<p><p>Researchers attribute increased incidence of colorectal cancer to environmental pollution with endocrine disruptors, including the estrogen-like substance bisphenol A (BPA). The proliferative activity, viability, and mRNA expression levels of the PIK3CA, NFkBp65, VEGFA, ESR1, HIF1a, PTEN, TP53, CD44, and CDH1 genes were evaluated in human colon adenocarcinoma HT-29 cell culture incubated with different concentrations of BPA (5, 25, and 50 μM) for 24 and 96 h. Short-term (24 h) incubation with BPA did not lead to pronounced biological effects. During prolonged incubation (96 h), 5 μM BPA decreased VEGFA mRNA expression and increased HIF1a mRNA expression, 25 μM BPA increased the expression of PIK3CA, ESR1, and HIF1a mRNAs and decreased VEGFA and CD44, and 50 μM BPA increased the expression of ESR1 mRNA and was cytotoxic. Hence, the duration of incubation and the concentration of BPA in the culture medium affect the progression of the colon cancer and its malignancy.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"135-140"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信