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Bulletin of Experimental Biology and Medicine最新文献

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Differentiation of the Cytokine Profile Modified by SARS-CoV-2 and Influenza Virus in an In Vitro Experiment. SARS-CoV-2和流感病毒修饰细胞因子谱的体外分化实验
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06430-x
N V Zaitseva, K G Starkova, O V Dolgikh, V B Alekseev
{"title":"Differentiation of the Cytokine Profile Modified by SARS-CoV-2 and Influenza Virus in an In Vitro Experiment.","authors":"N V Zaitseva, K G Starkova, O V Dolgikh, V B Alekseev","doi":"10.1007/s10517-025-06430-x","DOIUrl":"10.1007/s10517-025-06430-x","url":null,"abstract":"<p><p>We compared changes in the expression of the cytokine profile modified by SARS-CoV-2 and influenza virus in an in vitro experiment. A mixed population of immunocytes (T-helpers, B cells, NK cells, cytotoxic T lymphocytes, and monocytes) was isolated from peripheral venous blood samples (n = 32) of conditionally healthy male donors aged 25-35 years. The modifying factors were recombinant adenovirus particles of 26 and 5 serotypes containing the gene encoding S protein of SARS-CoV-2, as well as vaccine antigens (hemagglutinins) of influenza virus type A (H1N1 and H2N3) and type B. Multidirectional changes in the cytokine production was revealed upon exposure to SARS-CoV-2 antigens: stimulation of the expression of IL-1β and IL-8 and suppression of the production of IL-4 and IL-6; changes in the expression of IL-8 and IL-6 were more pronounced after combined exposure (SARS-CoV-2 + influenza virus). Influenza virus antigens predominantly stimulated the expression (IL-8), while the maximum cytokine-producing effects were noted after combined exposure to SARS-CoV-2 and influenza virus antigens. The obtained results extend the understanding of the pathogenic mechanisms associated with persistence of SARS-CoV-2 and influenza virus. The modifications of the cytokine profile produced by the viruses and verified in vitro allow predicting and monitoring the course of diseases.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"54-57"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Ethylmethylhydroxypyridine Succinate on the Expression of PGC-1α, GR, SUCNR1, and SDHA Genes in the Cerebral Cortex of Old Rats during a Course of Dexamethasone Administration. 琥珀酸乙基甲基羟吡啶对老龄大鼠皮质PGC-1α、GR、SUCNR1和SDHA基因表达的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06431-w
O L Terekhina, Yu I Kirova
{"title":"Effect of Ethylmethylhydroxypyridine Succinate on the Expression of PGC-1α, GR, SUCNR1, and SDHA Genes in the Cerebral Cortex of Old Rats during a Course of Dexamethasone Administration.","authors":"O L Terekhina, Yu I Kirova","doi":"10.1007/s10517-025-06431-w","DOIUrl":"10.1007/s10517-025-06431-w","url":null,"abstract":"<p><p>We assessed the transcriptional activity of the genes PGC-1α, GR, SUCNR1, and SDHA in the cerebral cortex of old (age 18 months) male rats receiving course administration of dexamethasone alone and in combination with ethylmethylhydroxypyridine succinate. According to real-time PCR data, the course intraperitoneal administration of the glucocorticoid receptor (GRα) agonist dexamethasone (1 mg/kg, daily for 10 days) reduced the expression of the PGC-1α, GR, SUCNR1, and SDHA genes in rat cerebral cortex. Intraperitoneal administration of ethylmethylhydroxypyridine succinate (100 mg/kg, daily for 10 days) after each dexamethasone injection normalized the expression of PGC-1α and SUCNR1 genes and significantly activated the expression of GR and SDHA. Thus, succinate/SUCNR1-mediated activation of the PGC-1α, GR, SUCNR1, and SDHA genes was detected for the first time, in the cerebral cortex of old rats during chronic stimulation of GRα with supraphysiological doses of dexamethasone.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"58-63"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of Cell Models with Stable Expression of a Caspase-3 Activity Fluorescent Sensor. 稳定表达Caspase-3活性荧光传感器的细胞模型的生成。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06450-7
Y S Kim, N N Bagmet, S L Malov, G V Manukyan, K N Yarygin, I V Kholodenko
{"title":"Generation of Cell Models with Stable Expression of a Caspase-3 Activity Fluorescent Sensor.","authors":"Y S Kim, N N Bagmet, S L Malov, G V Manukyan, K N Yarygin, I V Kholodenko","doi":"10.1007/s10517-025-06450-7","DOIUrl":"10.1007/s10517-025-06450-7","url":null,"abstract":"<p><p>A lentiviral vector was created for inserting the commercial fluorogenic protease biosensor for caspase-3, ZipGFP-Casp3, into cell cultures. Three cultures of mesenchymal stem cells (MSCs) from the liver and placenta, dermal fibroblasts, and colorectal carcinoma clones of HT-29 and Caco2 cell lines with knockout of the main cancer stem cell marker CD133, along with control clones of these lines, were selected as the target cultures for studying apoptosis. All cultures were transduced with the ZipGFP-Casp3 sensor. The transduced cells were sorted, and in some derived cultures, cells undergoing spontaneous apoptosis and showing stable GFP fluorescence were noted. Additionally, experiments were conducted to study the sensitivity of transduced placental mesenchymal stem cells, as well as clones of the HT-29 line with CD133 knockout and its control clone, to classical apoptosis inducers staurosporine and TRAIL. The obtained results confirm that the derived cell cultures are good models for studying apoptosis in MSC and in the cancer stem cell population.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"162-169"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Study of Cognitive Functions of Rats with Experimental Alzheimer's Disease after Transplantation of Transduced Olfactory Ensheathing Cells Expressing Nerve Growth Factor. 表达神经生长因子的转导嗅鞘细胞移植对实验性阿尔茨海默病大鼠认知功能的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06446-3
E K Karsuntseva, A D Voronova, V S Shishkina, V Yu Pokidko, A I Mukhina, A V Chadin, G A Fursa, A V Fedorov, O V Stepanova, V P Chekhonin
{"title":"The Study of Cognitive Functions of Rats with Experimental Alzheimer's Disease after Transplantation of Transduced Olfactory Ensheathing Cells Expressing Nerve Growth Factor.","authors":"E K Karsuntseva, A D Voronova, V S Shishkina, V Yu Pokidko, A I Mukhina, A V Chadin, G A Fursa, A V Fedorov, O V Stepanova, V P Chekhonin","doi":"10.1007/s10517-025-06446-3","DOIUrl":"10.1007/s10517-025-06446-3","url":null,"abstract":"<p><p>We evaluated the efficiency of transplantation of a gene-cell construct based on olfactory ensheathing cells expressing neurotrophic NGF in female Wistar rats with an experimental model of Alzheimer's disease. NGF improved long-term memory and learning ability in the passive avoidance test at week 3 after cell transplantation.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"127-134"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Local Hemostatic and Wound-Healing Agent in a Liquid Dosage Form. 局部止血和伤口愈合液体剂型。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06428-5
T G Khonina, S G Alekseenko, M N Dobrinskaya, N V Izmozherova, L P Larionov, D S Tishin, E V Shadrina, V M Frolova, A V Zamyatin, O N Chupakhin
{"title":"Local Hemostatic and Wound-Healing Agent in a Liquid Dosage Form.","authors":"T G Khonina, S G Alekseenko, M N Dobrinskaya, N V Izmozherova, L P Larionov, D S Tishin, E V Shadrina, V M Frolova, A V Zamyatin, O N Chupakhin","doi":"10.1007/s10517-025-06428-5","DOIUrl":"10.1007/s10517-025-06428-5","url":null,"abstract":"<p><p>We propose a composition of a local hemostatic and wound-healing agent in a liquid dosage form, a water-glycerol colloidal suspension containing active substances (silicon and iron glycerolates and chitosan) and an auxiliary substance polyvinylpyrrolidone. The agent is nontoxic and exhibits high hemostatic activity (that was practically the same at different contents of iron (III) monoglycerolate (0.40 and 0.80 wt%)) and pronounced wound-healing properties.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"41-44"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomic Changes in Mouse Lung Tissue after a Long-Term Space Flight. 长期太空飞行后小鼠肺组织的转录组变化。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06422-x
D A Yakubets, Yu V Rudimova, L B Buravkova
{"title":"Transcriptomic Changes in Mouse Lung Tissue after a Long-Term Space Flight.","authors":"D A Yakubets, Yu V Rudimova, L B Buravkova","doi":"10.1007/s10517-025-06422-x","DOIUrl":"10.1007/s10517-025-06422-x","url":null,"abstract":"<p><p>Long-term space flights induce adaptive changes in almost all tissues and organs. To elucidate adaptive mechanisms at the molecular and cellular levels, it is important to analyze transcriptional shifts in response to weightlessness/microgravity. Here we examined the transcriptomic profile of mouse lung tissue after a long-term space mission as part of the NASA Rodent Research scientific program. We identified 45 differentially expressed genes. G-protein-coupled olfactory and vomeronasal receptor encoding genes were found to be \"ectopically\" upregulated. Most of the downregulated genes are responsible for protein folding and regulation of circadian rhythms. The obtained data confirm the low level of gravity sensitivity of lung cells and their high adaptive potential under conditions of prolonged space flight.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"6-9"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-Dependent Changes in Mitochondrial Regulatory Mechanisms in the Spinal Cord of SOD1-G93A-Transgenic Mice with the Phenotype of Amyotrophic Lateral Sclerosis. 肌萎缩性侧索硬化表型sod1 - g93a转基因小鼠脊髓线粒体调控机制的年龄依赖性变化
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06429-4
N V Belosludtseva, I B Mikheeva, V S Starinets, M V Dubinin, K N Belosludtsev
{"title":"Age-Dependent Changes in Mitochondrial Regulatory Mechanisms in the Spinal Cord of SOD1-G93A-Transgenic Mice with the Phenotype of Amyotrophic Lateral Sclerosis.","authors":"N V Belosludtseva, I B Mikheeva, V S Starinets, M V Dubinin, K N Belosludtsev","doi":"10.1007/s10517-025-06429-4","DOIUrl":"10.1007/s10517-025-06429-4","url":null,"abstract":"<p><p>Age-dependent changes in the expression level of genes encoding proteins responsible for mitochondrial homeostasis were studied in relation to ultrastructural abnormalities in the mitochondria of motor neurons in the anterior horns of the spinal cord in a transgenic model of amyotrophic lateral sclerosis (SOD1-G93A mice). The expression of the Drp1, Mfn2, Ppargc1a, and Nefl genes was reduced, and the expression of the Nfe2l2, Pink1, and Parkin genes was enhanced in mice with the genotype of the familial form of the disease at the age of 22 weeks corresponding to the symptomatic stage in comparison with wild-type mice (C57BL6 × SJL) and non-transgenic littermates (SOD1-G93A(Tg-)) of the same age. Comparative analysis of spinal cord tissue samples from 8 and 12 weeks-old animals revealed no significant differences in the expression levels of genes encoding proteins responsible for mitochondrial dynamics, biogenesis, and mitophagy. Electron microscopic examination showed pronounced structural alterations in mitochondria in the soma of lower motor neurons of SOD1-G93A(Tg+) mice at the symptomatic stage, which manifested in the appearance of \"ring-like\" mitochondrial structures, matrix swelling, destruction of membranes in the cristae, and increased number of autophagolysosomes. The role of mitochondrial homeostasis disorders in the progression of amyotrophic lateral sclerosis is discussed.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"34-40"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Extracellular Genomic and Mitochondrial DNA in Follicular Fluid of Women Depending on the Type of Final Oocyte Maturation Trigger in ART Programs. 基于ART方案中最终卵母细胞成熟触发类型的女性卵泡液细胞外基因组和线粒体DNA评估
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06445-4
B V Zingerenko, N P Makarova, D N Kokoeva, M G Kosenko, L E Sorokina, E A Kalinina, A M Krasny
{"title":"Assessment of Extracellular Genomic and Mitochondrial DNA in Follicular Fluid of Women Depending on the Type of Final Oocyte Maturation Trigger in ART Programs.","authors":"B V Zingerenko, N P Makarova, D N Kokoeva, M G Kosenko, L E Sorokina, E A Kalinina, A M Krasny","doi":"10.1007/s10517-025-06445-4","DOIUrl":"10.1007/s10517-025-06445-4","url":null,"abstract":"<p><p>We compared the content of extracellular genomic and mitochondrial DNA (ec-gDNA and ec-mtDNA) in the follicular fluid (FF) of women after using two types of triggers for final oocyte maturation: human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa). The study included 43 women aged 21-35 years with preserved ovarian reserve according to hormonal profile and ultrasound examination, who were divided into two groups depending on the type of trigger: hCG at a dose of 10,000 IU (group 1; n = 30) and GnRHa at a dose of 0.2 mg (group 2; n = 13). From each patient, a sample of FF was obtained during transvaginal puncture from a dominant follicle without blood admixture. The content of ec-gDNA and ec-mtDNA was measured by qPCR. At the first stage, a positive correlation was found between the content of ec-gDNA and ec-mtDNA and the level of anti-Müllerian hormone, as well as between the level of ec-mtDNA and the number of oocytes. In patients reveiving GnRHa, the level of ec-mtDNA was significantly higher. There were no significant differences between the groups in the level of free ec-gDNA. The identified differences in ec-mtDNA in FF not only indicate different mechanisms of action of the used drugs, but also offer hope for the development of more precise approaches to the use of FF at the embryological stage of ART infertility treatment programs.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"123-126"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effects of Threefold 30-Minute Inhalation Sessions of an Argon-Oxygen Mixture on a Photochemically Induced Ischemic Stroke Model in Rats. 三次30分钟吸入氩氧混合物对光化学诱导的大鼠缺血性卒中模型的影响。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06427-6
M V Sutormin, E A Boeva, D N Silachev, E I Yakupova, V V Antonova, M A Lubomydrov, S N Kalabushev, A V Erchov, O A Grebenchikov, A N Kuzovlev
{"title":"The Effects of Threefold 30-Minute Inhalation Sessions of an Argon-Oxygen Mixture on a Photochemically Induced Ischemic Stroke Model in Rats.","authors":"M V Sutormin, E A Boeva, D N Silachev, E I Yakupova, V V Antonova, M A Lubomydrov, S N Kalabushev, A V Erchov, O A Grebenchikov, A N Kuzovlev","doi":"10.1007/s10517-025-06427-6","DOIUrl":"10.1007/s10517-025-06427-6","url":null,"abstract":"<p><p>Studies on rats have demonstrated that successive threefold 30-min sessions of inhalation of an argon-oxygen mixture (Ar 70%/O<sub>2</sub> 30%) immediately after and on days 2 and 3 after ischemic stroke reduce neurological deficit, the area of vWF<sup>+</sup> staining in brain sections, and the number of Iba-1<sup>+</sup> and Cas-3<sup>+</sup> cells in the lesion focus and NeuN<sup>+</sup> cells in the penumbra. However, no differences in the lesion volume were detected by MRI. Argon-oxygen inhalations reduced neurological deficits through their influence on reparative processes within the lesion site.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"14-19"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impairment of P2 Receptor-Mediated Modulation of Skeletal Muscle Contractions in Transgenic Mice with Modeled Amyotrophic Lateral Sclerosis. P2受体介导的骨骼肌收缩调控在肌萎缩性侧索硬化症转基因小鼠中的损害
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06425-8
A E Khairullin, D V Efimova, A Y Teplov, A N Khabibrakhmanov, K K Nagiev, S N Grishin, A U Ziganshin, M A Mukhamedyarov
{"title":"Impairment of P2 Receptor-Mediated Modulation of Skeletal Muscle Contractions in Transgenic Mice with Modeled Amyotrophic Lateral Sclerosis.","authors":"A E Khairullin, D V Efimova, A Y Teplov, A N Khabibrakhmanov, K K Nagiev, S N Grishin, A U Ziganshin, M A Mukhamedyarov","doi":"10.1007/s10517-025-06425-8","DOIUrl":"10.1007/s10517-025-06425-8","url":null,"abstract":"<p><p>We studied the effects of ATP on skeletal muscle contractility in FUS-transgenic mice with amyotrophic lateral sclerosis (ALS) model. Mechanomyography showed that ATP application did not increase the amplitude of electrically induced contractions of the diaphragm muscle, m. extensor digitorum longus, and soleus muscle in FUS-transgenic mice unlike wild-type mice. Application of a non-selective P2 receptor antagonist suramine and application of ATP against the background of suramine did not change the amplitude of contractions of the studied skeletal muscles in FUS-transgenic mice. Thus, FUS model of ALS is based on the impairment of purinergic regulation of skeletal muscle contractile activity, which can play a role in the pathogenesis of ALS.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"20-23"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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