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Indicators of Endogenous Intoxication in Uncomplicated Pregnancy. Medium-Weight Molecules and Lipid Peroxidation Products. 无并发症妊娠中内源性中毒的指标。中等质量分子和脂质过氧化产物。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06390-2
N V Semenova, O A Nikitina, E A Novikova, A N Karacheva, A Yu Marianian, S I Kolesnikov, A V Labygina, L I Kolesnikova
{"title":"Indicators of Endogenous Intoxication in Uncomplicated Pregnancy. Medium-Weight Molecules and Lipid Peroxidation Products.","authors":"N V Semenova, O A Nikitina, E A Novikova, A N Karacheva, A Yu Marianian, S I Kolesnikov, A V Labygina, L I Kolesnikova","doi":"10.1007/s10517-025-06390-2","DOIUrl":"https://doi.org/10.1007/s10517-025-06390-2","url":null,"abstract":"<p><p>The endogenous intoxication level was assessed based on the content of medium-weight molecules (MWM) and LPO products in the blood serum of women with uncomplicated pregnancy depending on the gestational age: 6-12, 18-22, 28-32, and 38-40 weeks. A significant increase in the MWM level at λ = 280 nm was found at weeks 28-32 compared to the second trimester (p = 0.05), as well as before delivery compared to the first trimester (p = 0.013). In the third trimester of pregnancy and before delivery, the substrates with double bonds and diene conjugates content increased (p < 0.05), but the levels of highly toxic TBA-active products before delivery did not differ from that at the beginning of the gestation process. These phase changes in LPO processes correspond to changes in the hormonal status of the body during uncomplicated pregnancy, and MWM measured at λ = 280 nm is a sensitive marker for monitoring of the endogenous intoxication level.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"643-646"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production of IL-1β and IL-10 by Blood Cells of Rats before and One Month after Sublethal Hypoxic Exposure in a Decompression Chamber. 减压室亚致死低氧暴露前后1个月大鼠血细胞产生IL-1β和IL-10的变化
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06395-x
D Sh Dzhalilova, A M Kosyreva, M V Silina, M A Mayak, I S Tsvetkov, O V Makarova
{"title":"Production of IL-1β and IL-10 by Blood Cells of Rats before and One Month after Sublethal Hypoxic Exposure in a Decompression Chamber.","authors":"D Sh Dzhalilova, A M Kosyreva, M V Silina, M A Mayak, I S Tsvetkov, O V Makarova","doi":"10.1007/s10517-025-06395-x","DOIUrl":"https://doi.org/10.1007/s10517-025-06395-x","url":null,"abstract":"<p><p>Spontaneous and stimulated by hypoxic conditions and a complex of mitogens (LPS, PHA, and ConA) production of IL-1β and IL-10 by peripheral blood cells of adult male Wistar rats 2 weeks before and 1 month after sublethal hypoxic exposure was examined to determine their tolerance to oxygen deficiency in a decompression chamber. To analyze spontaneous cytokine production, heparinized blood cells were incubated in culture medium for 1 and 24 h (37°C, 21% O<sub>2</sub> and 5% CO<sub>2</sub>). To study cytokine production stimulated by hypoxia, heparinized blood cells were incubated in culture medium for 1 h (37°C, 1% O<sub>2</sub> and 5% CO<sub>2</sub>), and cytokine production stimulated by a complex of mitogens was analyzed by incubating heparinized blood cells in medium supplemented with LPS, PHA, and ConA for 24 h (37°C, 21% O<sub>2</sub> and 5% CO<sub>2</sub>). The levels of proinflammatory cytokine IL-1β and anti-inflammatory cytokine IL-10 in a culture medium were measured by ELISA. The response to hypoxic stimulation and exposure to complex mitogen after sublethal hypoxic exposure in the decompression chamber differs from that before it in high (HR) and low resistant (LR) to hypoxia animals. After hypoxic stimulation and exposure to complex mitogen, the production of proinflammatory cytokine IL-1β increases in both HR and LR rats, but the production of anti-inflammatory IL-10 decreases in LR animals and does not change in HR rats. This indicates the formation of a proinflammatory phenotype in LR animals after sublethal hypoxic exposure. The obtained data should be taken into account when conducting studies using animals with different tolerance to hypoxia.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"670-674"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hepatoprotective Properties of New Xymedon Derivatives with a Modified Structural Moiety. 结构片段修饰的新型西梅酮衍生物的保肝性能。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06383-1
A B Vyshtakalyuk, A A Parfenov, V E Semenov, M S Shashin, I V Galyametdinova, M V Kriulina, V V Zobov
{"title":"Hepatoprotective Properties of New Xymedon Derivatives with a Modified Structural Moiety.","authors":"A B Vyshtakalyuk, A A Parfenov, V E Semenov, M S Shashin, I V Galyametdinova, M V Kriulina, V V Zobov","doi":"10.1007/s10517-025-06383-1","DOIUrl":"https://doi.org/10.1007/s10517-025-06383-1","url":null,"abstract":"<p><p>We studied the hepatoprotective properties of new modifications of the Russian drug, the regeneration stimulator Xymedon (1,2-dihydro-4,6-dimethyl-1-(2-hydroxyethyl)-pyrimidin-2-one) (1): 1,2-dihydro-4,6-dimethyl-(1-(2-((hydroxyethyl)amino)ethyl)-pyrimidin-2-one (2) and 1,2-dihydro-4,6-dimethyl-1-propyl-pyrimidin-2-one (3). First, cytotoxicity and cytoprotective effects of the compounds were assessed on Chang Liver, HepG2, and WI-38 human cell lines, and then, hepatoprotective activity in vivo was evaluated on the model of toxic CCl<sub>4</sub>-induced liver damage in Wistar rats. Compounds (2) and (3) exhibited cytoprotective properties: they increased cell viability against the background of d-galactosamine treatment and reduced the morphological signs of liver damage caused by CCl<sub>4</sub>. Compound (3) had significantly higher cytotoxicity. Of the two modifications, compound (2) is more promising for further work.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"605-609"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological Changes in the Walls of Arteries of Different Basins at the Early Stages of Atherosclerosis Development. 不同盆地动脉壁在动脉粥样硬化发展早期的形态变化。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-29 DOI: 10.1007/s10517-025-06393-z
B A Pospelov, I S Murashov, A M Volkov, M G Klinnikova, E L Lushnikova
{"title":"Morphological Changes in the Walls of Arteries of Different Basins at the Early Stages of Atherosclerosis Development.","authors":"B A Pospelov, I S Murashov, A M Volkov, M G Klinnikova, E L Lushnikova","doi":"10.1007/s10517-025-06393-z","DOIUrl":"https://doi.org/10.1007/s10517-025-06393-z","url":null,"abstract":"<p><p>Pathomorphological, immunohistochemical, and morphometric studies were performed on samples of coronary, common carotid, renal, and common iliac arteries from 32 patients (95 samples) who died at the age of 40-70 years without terminal atherosclerosis (vascular stenosis < 35%). In all arterial samples, uneven thickening of the intima was observed due to the formation of large areas of fibrosis and hyperelastosis. The formation of atheromatous plaque with numerous small deposits of lipid detritus was detected in 24 (25.3%) samples. Atheromatous foci were more common in coronary arteries and less common in the renal arteries. The largest intimal area on cross-sections was found in the common iliac arteries (33-34% larger than the in coronary and common carotid arteries, respectively, and 63% larger in the renal arteries). The area of the media was maximum in common carotid and common iliac arteries, and minimum in coronary arteries. The intima-to-media area ratio was maximum in the coronary arteries. A strong correlation was found between the intima area and the number of newly formed vessels (r = 0.733, p < 0.05) and between the number of vessels and macrophages (r = 0.675, p < 0.01). The frequency of pronounced fibrosis of the media (area of fibrous changes ⩾ 50%) was highest in fragments of renal arteries and lower in carotid artery samples. The greatest number of calcification foci was observed in samples of the common iliac arteries (35% of the total number, p < 0.01). The obtained results demonstrate both stereotypy and heterogeneity of atherosclerotic lesions in arteries from different vascular basins.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"661-665"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometry of the Human Ventral Germinal Matrix during Fetal Development. 胎儿发育过程中人腹侧生发基质的形态测定。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-29 DOI: 10.1007/s10517-025-06396-w
A E Proshchina, E O Grushetskaya, A S Kharlamova, O S Godovalova, Yu S Krivova, S V Saveliev
{"title":"Morphometry of the Human Ventral Germinal Matrix during Fetal Development.","authors":"A E Proshchina, E O Grushetskaya, A S Kharlamova, O S Godovalova, Yu S Krivova, S V Saveliev","doi":"10.1007/s10517-025-06396-w","DOIUrl":"https://doi.org/10.1007/s10517-025-06396-w","url":null,"abstract":"<p><p>The most important transient structures in human brain are the lateral ventricle eminences, or ganglionic eminences (eminentia ventricularis lateralis), the ventral part of germinal matrix. We performed quantitative analysis of the lateral ventricle eminences to clarify the spatial and temporal dynamic of the human prenatal brain development. Serial brain sections from 9 human fetuses at gestational weeks 12-30 were examined using a morphometric method. The volume of ganglionic eminences increased during the first and early second trimesters, reached maximum at weeks 20-21, and then gradual involution of the ventral germinal matrix was observed. The obtained data are comparable with the results from noninvasive imaging techniques, but have a higher resolution.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"675-680"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Telomere Length of Cardiomyocytes in Wistar Rat Treated with Doxorubicin: In Vivo Experimental Study. 阿霉素对Wistar大鼠心肌细胞端粒长度的体内实验研究。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-29 DOI: 10.1007/s10517-025-06381-3
M A Asanov, A O Poddubnyak, A V Sinitskaya, M V Khutornaya, O V Khryachkova, M Yu Sinitsky
{"title":"Telomere Length of Cardiomyocytes in Wistar Rat Treated with Doxorubicin: In Vivo Experimental Study.","authors":"M A Asanov, A O Poddubnyak, A V Sinitskaya, M V Khutornaya, O V Khryachkova, M Yu Sinitsky","doi":"10.1007/s10517-025-06381-3","DOIUrl":"https://doi.org/10.1007/s10517-025-06381-3","url":null,"abstract":"<p><p>The effect of doxorubicin on the relative telomere length of cardiomyocytes in Wistar rats was studied. Doxorubicin (2 mg/kg body weight) was injected into the caudal vein once a week for 4 weeks, the control group was injected with 0.9% NaCl solution in an equivalent volume. The serum concentrations of proinflammatory cytokines (MCP-1 and TNFα), oxidative stress marker 8-hydroxydeoxyguanosine (8-OHdG), and telomerase reverse transcriptase (TERT) were measured. In rats treated with doxorubicin, the relative length of cardiomyocyte telomeres and serum levels of 8-OHdG were higher than the in control. Thus, molecular effects of subchronic low-dose doxorubicin exposure in rats were revealed: telomeric regions of cardiomyocyte DNA were lengthened, and the level of oxidative stress marker increased.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"597-600"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histostructure of the Hippocampus in Male and Female Wistar Rats with Diet-Induced Visceral Obesity and after Diet Correction. 饮食性内脏性肥胖及饮食矫正后雌雄Wistar大鼠海马组织结构的变化。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-29 DOI: 10.1007/s10517-025-06391-1
T E Kuznetsova, T A Mityukova, A A Basalai, M A Darenskaya, L V Rychkova, S I Kolesnikov, N V Semenova, L I Kolesnikova
{"title":"Histostructure of the Hippocampus in Male and Female Wistar Rats with Diet-Induced Visceral Obesity and after Diet Correction.","authors":"T E Kuznetsova, T A Mityukova, A A Basalai, M A Darenskaya, L V Rychkova, S I Kolesnikov, N V Semenova, L I Kolesnikova","doi":"10.1007/s10517-025-06391-1","DOIUrl":"https://doi.org/10.1007/s10517-025-06391-1","url":null,"abstract":"<p><p>We studied the histological structure of the hippocampus in male and female Wistar rats kept on a standard vivarium diet (StD) or a high-calorie diet (HCD) for 16 weeks. The effect of diet-induced visceral obesity correction was also evaluated. To this end, the rats receiving HCD for 8 weeks were transferred to StD for the next 8 weeks. HCD for 16 weeks led to diet-induced visceral obesity and disturbances in the histological structure of the hippocampus in both males and females. The most typical consequences of HCD were neurodegenerative signs, which appeared in different regions of the hippocampus. Transition to StD was effective not only in correcting visceral obesity, but also in leveling neurodegenerative changes in the structure of hippocampal neurons in male and female rats.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"647-653"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of the Adaptive Capacity of Growing Rats through Modification of the Protein Content in the Diet. 改变日粮蛋白质含量对生长大鼠适应能力的影响。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06394-y
N V Tyshko, N S Nikitin, S I Shestakova, A A Stankevich, O K Mustafina, G V Guseva, V A Tutelyan
{"title":"Modulation of the Adaptive Capacity of Growing Rats through Modification of the Protein Content in the Diet.","authors":"N V Tyshko, N S Nikitin, S I Shestakova, A A Stankevich, O K Mustafina, G V Guseva, V A Tutelyan","doi":"10.1007/s10517-025-06394-y","DOIUrl":"https://doi.org/10.1007/s10517-025-06394-y","url":null,"abstract":"<p><p>Hematological and morphological studies of the liver were carried out in a subacute experiment on rats receiving a model toxicant against the background of diets with decreasing protein content during the most important period of ontogenesis characterized by intensive growth and sexual maturation. It was found that the stimulating effect of hormesis manifested in the blood and liver under conditions of sufficient protein supply (⩾ 9% of total caloric content). Growing animals chronically exposed to protein deficiency (3-6%) were characterized by suppressed hemopoiesis and decreased size of hepatocytes; against the background of a toxic load, the suppression of hematopoiesis was even more pronounced. The toxicant had a stimulating influence on the liver even in the presence of protein deficiency, which indicates the priorities in distribution of resource under conditions of adaptation to stress.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"666-669"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Study of Cancer Stem Cells and Reprogrammed CD3+CD8+ T Cells: the Search for Personalized Diagnostics and Targeted Treatment of Small Cell Lung Cancer (Experience of a Pilot Study). 癌症干细胞和重编程CD3+CD8+ T细胞的体外研究：寻找小细胞肺癌的个性化诊断和靶向治疗（一项试点研究的经验）。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06388-w
E G Skurikhin, N N Ermakova, M A Zhukova, E S Pan, A A Kubatiev, S G Morozov, A M Dygai
{"title":"In Vitro Study of Cancer Stem Cells and Reprogrammed CD3<sup>+</sup>CD8<sup>+</sup> T Cells: the Search for Personalized Diagnostics and Targeted Treatment of Small Cell Lung Cancer (Experience of a Pilot Study).","authors":"E G Skurikhin, N N Ermakova, M A Zhukova, E S Pan, A A Kubatiev, S G Morozov, A M Dygai","doi":"10.1007/s10517-025-06388-w","DOIUrl":"https://doi.org/10.1007/s10517-025-06388-w","url":null,"abstract":"<p><p>The content of cancer stem cells and CD3<sup>+</sup>CD8<sup>+</sup> T cells in the blood of a patient with small cell lung cancer (SCLC) and chronic obstructive pulmonary disease (COPD) was evaluated. In vitro analysis of tumorospheres allowed us to characterize circulating tumor stem cells in the blood of patients at risk. The fundamental possibility of reprogramming exhausted CD3<sup>+</sup>CD8<sup>+</sup> T cells (rCD3CD8T) of a patient with SCLC and COPD using a mitogen-activated protein kinase inhibitor (iMEK) and human monoclonal antibody nivolumab was demonstrated. Target cell elimination by rCD3CD8T was achieved by treatment with autologous cancer cell and stem cancer cell lysate in vitro. Thus, blockade of the MAPK/ERK signaling pathway and the PD-1/PD-L1 checkpoints may be used to overcome exhaustion and enhance cytotoxicity of CD3<sup>+</sup>CD8<sup>+</sup> T cells in patients with SCLC and COPD.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"631-636"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biomolecular and Functional Changes in a Culture of Microglial Cells Caused by Long-Term Exposure to AlCl3. 长期暴露于AlCl3引起的小胶质细胞培养的生物分子和功能变化。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s10517-025-06392-0
A V Sentyabreva, M A Diatroptova, E A Miroshnichenko, A D Gerasimov, A M Kosyreva
{"title":"Biomolecular and Functional Changes in a Culture of Microglial Cells Caused by Long-Term Exposure to AlCl<sub>3</sub>.","authors":"A V Sentyabreva, M A Diatroptova, E A Miroshnichenko, A D Gerasimov, A M Kosyreva","doi":"10.1007/s10517-025-06392-0","DOIUrl":"https://doi.org/10.1007/s10517-025-06392-0","url":null,"abstract":"<p><p>Inflammaging is one of the main risk factors for the development and progression of age-related diseases. The increase in the proinflammatory background and ROS production can lead to persistent activation and dysfunction of microglial cells. The biomolecular and functional changes in microglial cells after long-term exposure to prooxidant aluminum chloride were studied in in vitro experiment. BV2 cells were cultured in the presence of 0.5 and 1 mM AlCl<sub>3</sub> for 70 or 140 h. The intensity of ROS production, apoptosis, and M1/M2 phenotype polarization of microglia were assessed by flow cytometry, qPCR-RT, and ELISA. Cells cultured with 0.5 mM AlCl<sub>3</sub> showed signs of \"healthy aging\", while the higher concentration (1 mM) of AlCl<sub>3</sub> led to persistent activation of microglia. The data obtained can be used for in vitro modeling of inflammaging and related physiological and pathological processes.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 5","pages":"654-660"},"PeriodicalIF":0.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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