Bulletin of Experimental Biology and Medicine最新文献_第4页

Retraction Note: Effect of Curcumin on Cognitive Behavior and Pathological Characteristics of the Hippocampus in Mice with Inherent Alzheimer's Disease. 撤稿说明：姜黄素对固有阿尔茨海默病小鼠认知行为和海马体病理特征的影响》（Retraction Note: Curcumin on Cognitive Behavior and Pathological Characteristics of the Hippocampus in Mice with Inherent Alzheimer's Disease）。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-12 DOI: 10.1007/s10517-024-06251-4

M J Zhang, J H Zhong, X J Yi

引用次数: 0

Effect of Experimental Litter Reduction on Cerebellum Development in Suckling Rats. 实验性减窝对乳鼠小脑发育的影响

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06270-1

B Ya Ryzhavskii, A V Lanshakova, Yu B Malofey

{"title":"Effect of Experimental Litter Reduction on Cerebellum Development in Suckling Rats.","authors":"B Ya Ryzhavskii, A V Lanshakova, Yu B Malofey","doi":"10.1007/s10517-024-06270-1","DOIUrl":"10.1007/s10517-024-06270-1","url":null,"abstract":"We studied morphological features of the cerebellum in 14-day-old Wistar rats from reduced litters (the number of pups was reduced from 10-12 to 6 on the next day after birth). The control group comprised 14-day-old animals from litters of medium size (10-12 rat pups). Rats from reduced litters had greater body weight and brain weight. The weight of the cerebellum, together with the weight of the adjacent part of the brain stem and the thickness of cerebellar cortex also significantly exceeded the corresponding parameters in control animals. The thickness of the molecular layer of the cerebellar cortex and the numerical density of Purkinje cells in these rats did not differ significantly from the control. The thickness of the external granular (neurogenic) layer of the cerebellar cortex in rats from reduced litters was smaller. This can indicate accelerated reduction of this layer that persists in rats until days 20-22 of age. Numerical density of cells in the external granular layer of control and experimental animals was similar. Numerical density Ki-67+ cells in this layer, as well as GFAP+ glial cells in the granular layer of the cerebellar cortex in rats from reduced litters significantly exceeded the corresponding parameters in control animals. The cerebellum of rats from litters reduced 1 day after birth had a number of differences in important indicators reflecting the rate of its development during the neonatal and suckling periods of ontogeny.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"797-801"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies. 使用多克隆抗体鉴定大鼠横纹肌中的 Obscurin 巨型异构体

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06259-w

Y V Gritsyna, V K Zhalimov, T A Uryupina, A D Ulanova, A G Bobylev, I M Vikhlyantsev

{"title":"Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies.","authors":"Y V Gritsyna, V K Zhalimov, T A Uryupina, A D Ulanova, A G Bobylev, I M Vikhlyantsev","doi":"10.1007/s10517-024-06259-w","DOIUrl":"10.1007/s10517-024-06259-w","url":null,"abstract":"Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. soleus (containing mainly slow fibers). The m. gastrocnemius, m. soleus, and diaphragm were found to have 2 giant isoforms of obscurin: a smaller A-isoform and a larger B-isoform. Their molecular weights were ~870 and ~1150 kDa in the diaphragm and m. gastrocnemius and ~880 and ~1130 kDa in m. soleus, respectively. The B-isoform to A-isoform ratio was 1:3 in the diaphragm and m. soleus and 1:4 in the m. gastrocnemius. In the left-ventricular myocardium, A-isoform of obscurin with a molecular weight of ~880 kDa was found. No other obscurin isoforms or their fragments within the molecular weight range of 10 up to ~800 kDa were revealed in the investigated rat striated muscles. The antibodies produced are recommended for research into qualitative and quantitative changes of giant obscurin isoforms in rat striated muscles in the norm and during the development of pathological processes.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"731-735"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Syndrome in Reproductive Age Women of Various Ethnic Groups. Neuroendocrine Status and Lipid Peroxidation System. 不同种族育龄妇女的代谢综合征。神经内分泌状态和脂质过氧化系统。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06254-1

L V Belenkaya, M A Darenskaya, S I Kolesnikov, L F Sholokhov, I N Danusevich, L M Lazareva, Ia G Nadeliaeva, L V Suturina, L I Kolesnikova

{"title":"Metabolic Syndrome in Reproductive Age Women of Various Ethnic Groups. Neuroendocrine Status and Lipid Peroxidation System.","authors":"L V Belenkaya, M A Darenskaya, S I Kolesnikov, L F Sholokhov, I N Danusevich, L M Lazareva, Ia G Nadeliaeva, L V Suturina, L I Kolesnikova","doi":"10.1007/s10517-024-06254-1","DOIUrl":"10.1007/s10517-024-06254-1","url":null,"abstract":"We analyzed the state of neuroendocrine regulation and LPO-antioxidant defense systems in reproductive age women with metabolic syndrome (MetS), representatives of the Russian and Buryat ethnic groups. Compared to the corresponding control groups, women from the Russian ethnic group with MetS had elevated levels of thyroid-stimulating hormone and free androgen index (FAI) and reduced levels of sex hormone-binding globulin, while women from the Buryat ethnic group had increased levels of prolactin and FAI. Changes in the LPO system in women of the Russian ethnic group with MetS consisted in an increase in the levels of substrates with double bonds, TBA-reactive substances, and fat-soluble vitamins. Buryat women with MetS had a higher content of primary oxidation products and reduced levels of glutathione. The results of the study indicate a hyperandrogenic shift in the neuroendocrine regulation system, as well as compensatory influences from different parts of the antioxidant defense system in women of reproductive age with MetS, depending on their ethnicity. These findings indicate the need for assessing and monitoring the levels of these metabolites in women with MetS, considering their ethnicity.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"705-710"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Lead, Cadmium, and Their Combination on the Heart Rate Regulation in Nonlinear Rats. 铅、镉及其组合对非线性大鼠心率调节的影响

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06271-0

A V Tryasuchev, V O Stupin, E V Kuryanova

{"title":"Effect of Lead, Cadmium, and Their Combination on the Heart Rate Regulation in Nonlinear Rats.","authors":"A V Tryasuchev, V O Stupin, E V Kuryanova","doi":"10.1007/s10517-024-06271-0","DOIUrl":"10.1007/s10517-024-06271-0","url":null,"abstract":"We studied heart rate variability (HRV) and the concentrations of heavy metals in the heart tissue after 14-fold peroral administration of heavy metal salts: lead acetate (50 mg/kg), cadmium chloride (5 mg/kg), and their combination. After administration of lead, its concentration in the myocardium exceeded the control values by 1.2 times or did not change in case of combination with cadmium; after administration of cadmium, its concentration in the myocardium exceeded the control value by 6.1 times or by 2 times after combined administration. After lead administration, a moderate increase in HR, low values of stress and centralization indices, and an increase in the power in all ranges of the HRV spectrum were recorded. After cadmium intoxication, a tendency to a decrease in heart rhythm variability, a moderate increase in HR, stress and centralization indices, and an increase in the contribution of VLF waves in the HRV spectrum were observed. After combined administration, a pronounced increase in HR and stress index and a moderate increase in the centralization of heart rhythm control were recorded, which was similar to changes in HRV observed after administration of cadmium alone. In the experiment, the concentration of cadmium in the myocardium increased to a much greater extent than the concentration of lead. After lead administration, the autonomous type of regulation persisted and parasympathetic influences increased. Cadmium apparently acts as a stress factor and increases the role of sympathoadrenal influences and central ergotropic structures in the heart rhythm regulation.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"751-756"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Markers Associated with Epithelial-Mesenchymal Transition and Extracellular Matrix Degradation in Human Uveal Melanoma. 人葡萄膜黑色素瘤中上皮-间质转化和细胞外基质降解相关标记物的表达

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06266-x

A Yu Shatruk, N P Bgatova, A V Yeremina, A N Trunov, V V Chernykh, Iu S Taskaeva

引用次数: 0

Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer's Patches of the Rat Intestine. 暴露于脂多糖后大鼠肠道佩耶氏斑的肠细胞顶端和滤泡相关上皮细胞的组织屏障的变化

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-31 DOI: 10.1007/s10517-024-06263-0

A A Fedorova, O V Rybalchenko, L S Okorokova, V V Kapustina, O G Orlova, A G Markov

{"title":"Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer's Patches of the Rat Intestine.","authors":"A A Fedorova, O V Rybalchenko, L S Okorokova, V V Kapustina, O G Orlova, A G Markov","doi":"10.1007/s10517-024-06263-0","DOIUrl":"10.1007/s10517-024-06263-0","url":null,"abstract":"To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer's patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers' patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"757-762"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Oxide (NO) in the Pathogenesis of Preeclampsia. 子痫前期发病机制中的氧化氮（NO）。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06255-0

V Yu Titov, A N Osipov, I V Babenkova, Yu O Teselkin, R I Shalina, Yu V Vykhristyuk, S Yu Lebedeva

引用次数: 0

Preparation and Construction of Chimeric Humanized Broadly Reactive Antibody 10H10 to Protein E of Tick-Borne Encephalitis Virus. 制备和构建针对蜱传脑炎病毒 E 蛋白的嵌合人源化广谱反应性抗体 10H10。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06265-y

D V Shanshin, V S Nesmeyanova, E V Protopopova, A A Shelemba, V B Loktev, D N Shcherbakov

{"title":"Preparation and Construction of Chimeric Humanized Broadly Reactive Antibody 10H10 to Protein E of Tick-Borne Encephalitis Virus.","authors":"D V Shanshin, V S Nesmeyanova, E V Protopopova, A A Shelemba, V B Loktev, D N Shcherbakov","doi":"10.1007/s10517-024-06265-y","DOIUrl":"10.1007/s10517-024-06265-y","url":null,"abstract":"A full-length humanized chimeric antibody 10H10ch that specifically interacts with the surface glycoprotein E of flaviviruses was obtained. To construct it, we used variable fragments of the heavy and light chains of the monoclonal antibody 10H10 that form the active center of the antibody and a fragment of the constant part of the heavy chain of the human IgG1 antibody. The resulting full-length chimeric humanized antibody 10H10ch specifically interacted with the E protein of flaviviruses pathogenic to humans, such as tick-borne encephalitis, Zika, West Nile, and dengue viruses. An immunochemical assessment of the interaction constants of the 10H10ch antibody with a panel of native and recombinant flavivirus antigens by ELISA and biolayer interferometry showed that the dissociation constant (Kd) of the chimeric antibody is in the nanomolar region and is comparable to that of the high-affinity mouse monoclonal antibody 10H10. The possibility of using the resulting chimeric humanized antibody 10H10ch for the diagnosis, prevention, and treatment of various flavivirus infections is discussed.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"770-773"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlations between Metabolic Parameters, Blood Cytokine Levels, and Behavioral Parameters in Male and Female Rats of Different Age after Intrauterine Stress. 不同年龄雌雄大鼠宫内应激后代谢参数、血液细胞因子水平和行为参数之间的相关性

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06252-3

A S Martyusheva, A Yu Subbotina, I V Alekseeva, A Yu Abramova, S S Pertsov

{"title":"Correlations between Metabolic Parameters, Blood Cytokine Levels, and Behavioral Parameters in Male and Female Rats of Different Age after Intrauterine Stress.","authors":"A S Martyusheva, A Yu Subbotina, I V Alekseeva, A Yu Abramova, S S Pertsov","doi":"10.1007/s10517-024-06252-3","DOIUrl":"10.1007/s10517-024-06252-3","url":null,"abstract":"We studied interrelationships between behavioral parameters, metabolic processes, and cytokine content in the blood of male and female rats at different stages of postnatal ontogeny after intrauterine stress (mothers were forced to swim in cold water from the 10th to the 16th day of pregnancy). Correlations between behavioral and metabolic parameters in prenatally stressed rats were revealed at an earlier age (day 21 of life) than in controls. In comparison with intact rats, in males exposed to intrauterine stress no relationships between these parameters were revealed at older age, while in females, their direction and character change on day 60 of life. In prenatally stressed males, correlations of the blood levels of IL-6 were revealed: inverse relationships with behavioral parameters on the days 21 and 30 and direct relationships with metabolic parameters on day 60 of life. In prenatally stressed females on day 30 of life, we observed negative correlations between IL-4 levels and metabolic parameters that were absent under normal conditions. Thus, intrauterine stress leads to reorganization of the relationships between the parameters of metabolic and immune processes, which are essential for the purposeful behavior of mammals. The effects of stress seem to depend on the sex of the offspring and stage of postnatal development.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"691-698"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0