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Bulletin of Experimental Biology and Medicine最新文献

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Functional Characteristics of Different Types of Structural Components (Biunites) of Intraorganic Venous Bed in the Spleen in Normal. 正常脾脏有机静脉床不同类型结构成分（双单元）的功能特征。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06468-x
A Sh Dadashev, O K Zenin, A A Sergienko, H M Bataev, I U Vagabov, E S Kafarov
{"title":"Functional Characteristics of Different Types of Structural Components (Biunites) of Intraorganic Venous Bed in the Spleen in Normal.","authors":"A Sh Dadashev, O K Zenin, A A Sergienko, H M Bataev, I U Vagabov, E S Kafarov","doi":"10.1007/s10517-025-06468-x","DOIUrl":"10.1007/s10517-025-06468-x","url":null,"abstract":"<p><p>Functional characteristics of different types of spleen venous bed biunites (BU) (by analogy with bifurcation) were studied by digital modeling based on the results of morphometry. Digital models of three different BU types were used: first (D < d<sub>max</sub> + d<sub>min</sub>), zero (D = d<sub>max</sub> + d<sub>min</sub>); second (D > d<sub>max</sub> + d<sub>min</sub>), where D is the diameter of the proximal, d<sub>max</sub> and d<sub>min</sub> are internal diameters of the distal segments. The ANSYS computer program was used to analyze the hemodynamic resistance and rigidity of the BU structure, and the Vasculograph software was used to analyze the coverage area. It was found that BU can be arranged as follows: by increasing hemodynamic resistance - first, second, and zero type BU; drainage function - second, zero, and first type BU; supporting function - zero, first, and second type BU. Different BU types make unequal contribution to different functions.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"250-253"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Tryptanthrin and Its Oxime on the Blood-Brain Barrier Permeability in Rats with Cerebral Infarction. 色氨酸及其肟对脑梗死大鼠血脑屏障通透性的影响。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06459-y
G A Chernysheva, V I Smolyakova, M B Plotnikov, O I Aliev, O A Ulyakhina, A N Osipenko, A R Kovrizhina, A I Khlebnikov
{"title":"The Effect of Tryptanthrin and Its Oxime on the Blood-Brain Barrier Permeability in Rats with Cerebral Infarction.","authors":"G A Chernysheva, V I Smolyakova, M B Plotnikov, O I Aliev, O A Ulyakhina, A N Osipenko, A R Kovrizhina, A I Khlebnikov","doi":"10.1007/s10517-025-06459-y","DOIUrl":"10.1007/s10517-025-06459-y","url":null,"abstract":"<p><p>A comparative study of the effect of tryptanthrin and its oxime (Tr-Ox) on the blood-brain barrier (BBB) permeability was conducted in male Wistar rats with cerebral infarction (CI) modeled by intraluminal occlusion of the middle cerebral artery. In 3, 48, and 72 h after CI, extravasation of Evans blue bound to serum albumin in the cerebral hemispheres was assessed 3 h after its intravenous administration (3 ml/kg, 2% solution). Tryptanthrin and Tr-Ox were injected intraperitoneally in a dose of 10 mg/kg on the 30th minute of ischemia and then daily for 2 days. There were no significant differences in the effects of these compounds 3 and 48 h after CI. In both experimental groups, the dye concentration in the left (affected) hemisphere significantly decreased after 3 and 48 h by 30-37 and 51-54%, respectively, in comparison with the control (CI without treatment); in the right hemisphere, dye concentration decreased by 34-39% after 48 h. After 72 h, 45% reduction in Evans blue extravasation in the affected hemisphere was found only in the Tr-Ox group. The ability of tryptanthrin and Tr-Ox to reduce the BBB permeability in the CI acute period is mainly due to the anti-inflammatory effect of these compounds. The protective effect of Tr-Ox, which can affect the JNK1/3 signaling pathway, was more prolonged.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"206-210"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes of α-Synuclein Localization in a Neuronal Precursor Graft in the Rat Model of 6-OHDA-Induced Parkinsonism. α-突触核蛋白在6-羟多巴胺诱导的帕金森大鼠神经前体移植物中的定位变化。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06469-w
D N Voronkov, A V Stavrovskaya, A S Olshanskiy, O S Lebedeva, S N Illarioshkin
{"title":"Changes of α-Synuclein Localization in a Neuronal Precursor Graft in the Rat Model of 6-OHDA-Induced Parkinsonism.","authors":"D N Voronkov, A V Stavrovskaya, A S Olshanskiy, O S Lebedeva, S N Illarioshkin","doi":"10.1007/s10517-025-06469-w","DOIUrl":"10.1007/s10517-025-06469-w","url":null,"abstract":"<p><p>In the experiment with transplantation of neuronal mesencephalic precursors derived from induced pluripotent stem cells into the striatum of rats with unilateral 6-OHDA destruction of the substantia nigra, we evaluated changes in the immunostaining for α-synuclein during differentiation and maturation of grafted neurons 1, 3, and 6 months after the surgery. The content of α-synuclein in transplanted neurons peaked by the third month after surgery, as well as changes in the nuclear-cytoplasmic ratio of α-synuclein in neurons by the 3rd and 6th months relative to the 1st month after transplantation were revealed. Changes in α-synuclein localization and content were associated with a gradual decrease in doublecortin expression and an increase in immunostaining for neuronal enolase and synaptophysin as the graft matured. The high content of total α-synuclein in developing neurons may be a key factor of spreading of α-synuclein toxic species in graft cells and a cause of cell damage.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"254-259"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the Safety of THz Irradiation on the Morphofunctional Characteristics of Rabbit Corneas. 太赫兹辐射对兔角膜形态功能特性的安全性评价。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06471-2
K Yu Krasner, E A Butikova, E V Drobot, N V Kanygin, I D Levchenko, V M Popik, D A Kolomeets, O I Solovieva, T G Tolstikova, E L Zavjalov, V V Chernykh, A N Trunov, V V Kanygin
{"title":"Assessment of the Safety of THz Irradiation on the Morphofunctional Characteristics of Rabbit Corneas.","authors":"K Yu Krasner, E A Butikova, E V Drobot, N V Kanygin, I D Levchenko, V M Popik, D A Kolomeets, O I Solovieva, T G Tolstikova, E L Zavjalov, V V Chernykh, A N Trunov, V V Kanygin","doi":"10.1007/s10517-025-06471-2","DOIUrl":"10.1007/s10517-025-06471-2","url":null,"abstract":"<p><p>The morphofunctional characteristics of rabbit corneas were studied after terahertz (THz) irradiation at a frequency of 2.3 THz with varying durations (15 or 30 min) or intensities (0.012 mW/cm<sup>2</sup> (38°C), 0.018 mW/cm<sup>2</sup> (40°C), 0.024 mW/cm<sup>2</sup> (42°C)) over a period of 1 week after exposure. The intensity of irradiation did not affect the functional changes in the eyes after exposure. However, in groups with different exposure durations, statistically significant changes in corneal hydration and endothelial cell density were observed, the extent of which depended on the exposure time. Endothelial cell density negatively correlated with corneal thickness (r = -0.36; p = 0.042), indicating a depletion of the endothelial cell pool associated with an increase in corneal thickness. These changes were subclinical in nature and did not lead to significant pathological changes in the cornea (no signs of hyperreflectivity were observed on optical coherence tomography of the anterior segment). Thus, the safety of THz irradiation at a frequency of 2.3 THz within the range of used intensities and exposure durations was confirmed in vivo study.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"266-269"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Glutamate Antibodies on ASCL1 Gene Expression in Aging Mice with Spatial Memory Impairment Caused by Amyloid Fibrils of the Proinflammatory Protein S100A9. 谷氨酸抗体对促炎蛋白S100A9淀粉样原纤维所致空间记忆损伤衰老小鼠ASCL1基因表达的影响
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06453-4
M A Gruden, T V Davydova, A M Ratmirov, L A Vetrile, Z I Storozheva
{"title":"Effect of Glutamate Antibodies on ASCL1 Gene Expression in Aging Mice with Spatial Memory Impairment Caused by Amyloid Fibrils of the Proinflammatory Protein S100A9.","authors":"M A Gruden, T V Davydova, A M Ratmirov, L A Vetrile, Z I Storozheva","doi":"10.1007/s10517-025-06453-4","DOIUrl":"10.1007/s10517-025-06453-4","url":null,"abstract":"<p><p>Immunological correction of cognitive processes impaired due to the action of neurotoxic amyloidogenic forms of proinflammatory protein S100A9, a promoter of the inflammatory-amyloid cascade occurring in Alzheimer's disease, is poorly understood. Chronic intranasal administration of S100A9 fibrils leads to suppression of spatial memory formation in the Morris water maze in 12-month-old C57BL/6J mice and to an increase in activity of the ASCL1 gene involved in neurogenesis at the stage of cell differentiation, in the hippocampus and prefrontal cortex. In the case of combined administration of S100A9 fibrillar structures and antibodies to glutamate, the duration of the latency of reaching the platform in the water maze as well as ASCL1 gene expression in the hippocampus and prefrontal cortex returned to normal, but not in the cerebellum where a decrease in ASCL1 gene activity was observed.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"177-180"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunogenicity of DNA Vaccines against COVID-19 That Encode B- and T-Cell Immunogens after Combined Injection. 联合注射编码B细胞和t细胞免疫原的COVID-19 DNA疫苗的免疫原性
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06461-4
M B Borgoyakova, A P Rudometov, E V Starostina, A M Zadorozhny, E V Tigeeva, V A Yakovlev, D N Kisakov, L A Kisakova, L I Karpenko, A A Ilyichev
{"title":"Immunogenicity of DNA Vaccines against COVID-19 That Encode B- and T-Cell Immunogens after Combined Injection.","authors":"M B Borgoyakova, A P Rudometov, E V Starostina, A M Zadorozhny, E V Tigeeva, V A Yakovlev, D N Kisakov, L A Kisakova, L I Karpenko, A A Ilyichev","doi":"10.1007/s10517-025-06461-4","DOIUrl":"10.1007/s10517-025-06461-4","url":null,"abstract":"<p><p>The possibility of enhancing the immune response to a DNA vaccine encoding an artificial polyepitope T-cell immunogen containing a large number of conserved epitopes from different proteins of SARS-CoV-2 virus (pBSI-COV-Ub) was assessed by additional introduction of a plasmid encoding the secreted receptor-binding domain of the of SARS-CoV-2 S protein (pVAXrbd). Both a specific humoral response and a cellular response of broad specificity were observed in animals co-injected with pBSI-COV-Ub and pVAXrbd. This indicates the potential of this approach as a vaccine strategy.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"217-222"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phosphatidylethanol 16:0/18:1PEth as a Biomarker of Alcohol Consumption during Pregnancy. 磷脂酰乙醇16:0/18:1PEth作为妊娠期间酒精消耗的生物标志物。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06466-z
E V Belyaeva, A N Karacheva, T A Bairova, N V Semenova, A V Belskikh, A Yu Marianian, E A Novikova, O A Nikitina, A Yu Sambyalova, O A Ershova, D P Tiumentceva, L I Kolesnikova
{"title":"Phosphatidylethanol 16:0/18:1PEth as a Biomarker of Alcohol Consumption during Pregnancy.","authors":"E V Belyaeva, A N Karacheva, T A Bairova, N V Semenova, A V Belskikh, A Yu Marianian, E A Novikova, O A Nikitina, A Yu Sambyalova, O A Ershova, D P Tiumentceva, L I Kolesnikova","doi":"10.1007/s10517-025-06466-z","DOIUrl":"10.1007/s10517-025-06466-z","url":null,"abstract":"<p><p>Plasma levels of 16:0/18:1PEth is as a laboratory blood marker of alcohol consumption. In a longitudinal cohort study, the plasma levels of 16:0/18:1PEth were measured in women at different gestational periods and the results were compared with the data from three alternative questionnaires of alcohol consumption during pregnancy. Pregnant women (n = 309) were surveyed using T-ACE, TWEAK, and AUDIT questionnaires followed by measurement of 16:0/18:1PEth by HPLC-MS. According to the survey, 61% of pregnant women had an experience of drinking alcohol before the onset of current pregnancy. Objectively, 60, 51, and 49% of involved women had blood plasma levels of 16:0/18:1PEth exceeding 8 ng/ml in the first, second, and third trimesters. The laboratory-confirmed frequency of alcohol consumption in pregnant women is significantly higher (p < 0.001) than in the survey.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"242-245"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ex Vivo Irradiation Protocol in Murine Lymph Node Cells. 小鼠淋巴结细胞的体外照射方案。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI: 10.1007/s10517-025-06465-0
M N Yurova, E I Fedoros, A B Danilova, T L Nekhaeva, N V Emelyanova, A V Grigorevskaya, I G Popovich, I A Baldueva
{"title":"Ex Vivo Irradiation Protocol in Murine Lymph Node Cells.","authors":"M N Yurova, E I Fedoros, A B Danilova, T L Nekhaeva, N V Emelyanova, A V Grigorevskaya, I G Popovich, I A Baldueva","doi":"10.1007/s10517-025-06465-0","DOIUrl":"10.1007/s10517-025-06465-0","url":null,"abstract":"<p><p>The aim was to test the protocol for obtaining lymphoid tissue from mouse lymph nodes and characterize the level of DNA damage with analysis of the repair and apoptosis protein markers in ex vivo model of cell irradiation. The lymph node cell suspension was exposed to X-ray irradiation at a dose of 2 Gy and compared with a sham-irradiated (0 Gy) control. Ex vivo irradiation at a dose of 2 Gy after 3 h caused a 7-fold increase in DNA damage level determined in DNA comet assay. A significant increase in the level of repair proteins γH2AX (by 3.7 times), Rad51 (by 2.2 times), and XPA (by 28 times), cell cycle arrest proteins Cdc25A (by 2.6 times), p21 (by 10 times), and p16 (by 5 times), and pro-apoptotic protein Bax (by 3.8 times) was noted, without significant changes in Bcl-2 level. The proposed technique allows quick obtaining lymphoid tissue samples suitable for analysis of the radiosensitivity of lymphoid cells in mice of various strains, changes in their sensitivity after or in combination with the primary damaging exposure.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"237-241"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methylene Blue Protects Spatial Working Memory after Traumatic Brain Injury in Mice by Affecting Mitochondrial Quality Control System. 亚甲基蓝通过影响线粒体质量控制系统保护小鼠创伤性脑损伤后的空间工作记忆。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06458-z
A P Gureev, P I Babenkova, V V Nesterova, D E Volodina, I S Sadovnikova, K A Starodubtsev, M E Ivanov, D N Silachev, E Yu Plotnikov
{"title":"Methylene Blue Protects Spatial Working Memory after Traumatic Brain Injury in Mice by Affecting Mitochondrial Quality Control System.","authors":"A P Gureev, P I Babenkova, V V Nesterova, D E Volodina, I S Sadovnikova, K A Starodubtsev, M E Ivanov, D N Silachev, E Yu Plotnikov","doi":"10.1007/s10517-025-06458-z","DOIUrl":"10.1007/s10517-025-06458-z","url":null,"abstract":"<p><p>The levels of inflammatory markers increased in both mouse blood plasma and affected brain area 24 days after traumatic brain injury, which was accompanied by impairment of spatial working memory. Methylene blue administered during the first 3 days after injury reduced the levels of some inflammation markers and increased the expression of genes involved in the regulation of mitochondrial biogenesis and mitophagy, i.e. genes responsible for mitochondrial quality control. Additionally, methylene blue partially mitigated the cognitive deficits induced by the injury, suggesting it as a promising compound for maintaining brain function after traumas.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"201-205"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Constructing of Synthetic DNA-Histone Networks as Extracellular Trap Model for Biomedical Research. 合成dna -组蛋白网络作为生物医学研究的细胞外陷阱模型的构建。
IF 0.6 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI: 10.1007/s10517-025-06473-0
E V Shmeleva, L Yu Basyreva, T V Vakhrusheva, A V Sokolov, S A Gusev, O M Panasenko, V I Sergienko
{"title":"Constructing of Synthetic DNA-Histone Networks as Extracellular Trap Model for Biomedical Research.","authors":"E V Shmeleva, L Yu Basyreva, T V Vakhrusheva, A V Sokolov, S A Gusev, O M Panasenko, V I Sergienko","doi":"10.1007/s10517-025-06473-0","DOIUrl":"10.1007/s10517-025-06473-0","url":null,"abstract":"<p><p>Extracellular traps (ETs) released during ETosis of various blood cells not only participate in host defense against pathogenic microorganisms, but also play an essential role in diverse pathological processes. It should be noted that ETs are heterogeneous by their structure; therefore, for studying their pathophysiological effects, it is extremely important to create model ETs with strictly controlled composition and structure. We propose an approach that allows creating model mesh structures based on DNA and histones (DNA-histone networks, DHN), which can be considered as model ETs. It has been shown that the structure of DHN depends on the DNA/histone weight ratio. The possibility of incorporating myeloperoxidase that can be present in native ETs, into DHN has been demonstrated. The creation of DHN with controlled composition and structure will presumably allow assessing the specificity of enzyme systems involved in the degradation of native ETs. In addition, these heterogeneous DHN can be used to study the relationship between ET structure and ET-induced thrombosis or their antimicrobial activity.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"277-281"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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