The Effect of Tryptanthrin and Its Oxime on the Blood-Brain Barrier Permeability in Rats with Cerebral Infarction.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI:10.1007/s10517-025-06459-y

G A Chernysheva, V I Smolyakova, M B Plotnikov, O I Aliev, O A Ulyakhina, A N Osipenko, A R Kovrizhina, A I Khlebnikov

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Abstract

A comparative study of the effect of tryptanthrin and its oxime (Tr-Ox) on the blood-brain barrier (BBB) permeability was conducted in male Wistar rats with cerebral infarction (CI) modeled by intraluminal occlusion of the middle cerebral artery. In 3, 48, and 72 h after CI, extravasation of Evans blue bound to serum albumin in the cerebral hemispheres was assessed 3 h after its intravenous administration (3 ml/kg, 2% solution). Tryptanthrin and Tr-Ox were injected intraperitoneally in a dose of 10 mg/kg on the 30th minute of ischemia and then daily for 2 days. There were no significant differences in the effects of these compounds 3 and 48 h after CI. In both experimental groups, the dye concentration in the left (affected) hemisphere significantly decreased after 3 and 48 h by 30-37 and 51-54%, respectively, in comparison with the control (CI without treatment); in the right hemisphere, dye concentration decreased by 34-39% after 48 h. After 72 h, 45% reduction in Evans blue extravasation in the affected hemisphere was found only in the Tr-Ox group. The ability of tryptanthrin and Tr-Ox to reduce the BBB permeability in the CI acute period is mainly due to the anti-inflammatory effect of these compounds. The protective effect of Tr-Ox, which can affect the JNK1/3 signaling pathway, was more prolonged.

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色氨酸及其肟对脑梗死大鼠血脑屏障通透性的影响。

采用脑中动脉腔内闭塞造模脑梗死雄性Wistar大鼠，比较研究了色氨酸及其肟（Tr-Ox）对血脑屏障（BBB）通透性的影响。在CI后3、48和72小时，静脉给药（3ml /kg， 2%溶液）3小时后，评估Evans蓝与大脑半球血清白蛋白结合的外渗情况。在缺血30分钟腹腔注射色氨酸和Tr-Ox，剂量为10 mg/kg，然后每天注射2 d。CI后3和48 h，这些化合物的作用无显著差异。在两个实验组中，与对照组（未处理CI）相比，3和48 h后左（受影响）半球的染料浓度分别显著降低了30- 37%和51-54%；右半球染色浓度48 h后下降34-39%。72 h后，仅Tr-Ox组患半球埃文斯蓝外渗减少45%。色氨酸和Tr-Ox在CI急性期降低血脑屏障通透性的能力主要是由于这些化合物的抗炎作用。Tr-Ox可影响JNK1/3信号通路，其保护作用更持久。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.