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Bulletin of Experimental Biology and Medicine最新文献

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Approbation of a Homologous Model of the Antitumor Vaccine Based on Mature Mouse Dendritic Cells to Study the Biodistribution of the Cell Product 批准基于成熟小鼠树突状细胞的抗肿瘤疫苗同源模型，以研究细胞产品的生物分布情况
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06226-5
T. L. Nekhaeva, I. D. Laskov, E. I. Fedoros, A. B. Danilova, M. N. Yurova, M. L. Tyndyk, E. D. Ermakova, N. V. Emelyanova, N. A. Efremova, A. V. Grigorevskaya, M. A. Nekrasova, I. A. Baldueva
{"title":"Approbation of a Homologous Model of the Antitumor Vaccine Based on Mature Mouse Dendritic Cells to Study the Biodistribution of the Cell Product","authors":"T. L. Nekhaeva, I. D. Laskov, E. I. Fedoros, A. B. Danilova, M. N. Yurova, M. L. Tyndyk, E. D. Ermakova, N. V. Emelyanova, N. A. Efremova, A. V. Grigorevskaya, M. A. Nekrasova, I. A. Baldueva","doi":"10.1007/s10517-024-06226-5","DOIUrl":"https://doi.org/10.1007/s10517-024-06226-5","url":null,"abstract":"<p>Homologous animal cell product was obtained in protocol developed for female BALB/c mice. Dendritic cell (DC) migration from the injection site into the draining lymph nodes was evaluated. The number of DC labeled with carboxyfluorescein succinimidyl ester (CFSE) in draining lymph nodes increased from 5.3% (16 h) to 13.3% (48 h) (<i>p</i>=0.028) with a maximum at 72 h (15.4%, <i>p</i>=0.003). The immunophenotype of CFSE-DC detected in murine lymph nodes corresponded to the immunophenotype of mature vaccine DCs: they expressed differentiation markers CD11c, CD80, CD83, and CD86 (<i>p</i>&gt;0.05 <i>vs</i> initial DC).</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Redox-Active Chitosan-Polyaminoxyl Micelles Loaded with Daunorubicin on Activity of Nrf2 Transcription Factor 载入多柔比星的具有氧化还原活性的壳聚糖-多氨氧胶束对 Nrf2 转录因子活性的影响
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06224-7
A. A. Balakina, V. I. Amozova, V. D. Sen’
{"title":"Influence of Redox-Active Chitosan-Polyaminoxyl Micelles Loaded with Daunorubicin on Activity of Nrf2 Transcription Factor","authors":"A. A. Balakina, V. I. Amozova, V. D. Sen’","doi":"10.1007/s10517-024-06224-7","DOIUrl":"https://doi.org/10.1007/s10517-024-06224-7","url":null,"abstract":"<p>A new system for delivery of anthracycline antibiotics based on chitosan-polyaminoxyls (CPA) was studied in a model of non-tumor (human embryonic mesenchymal stem cells) and tumor cells (human hepatocellular carcinoma) <i>in vitro</i>. The presence of CPA micelles considerably suppresses daunorubicin-induced ROS generation in normal cells without affecting this process in tumor cells. CPA micelles do not reduce the cytotoxic effect of daunorubicin and do not prevent its accumulation in cells. The use of CPA significantly increases accumulation of Nrf2 transcription factor in the nuclei of both normal and tumor cells in comparison with free daunorubicin. Increased nuclear translocation of Nrf2 leads to a significant increase in the expression of its target gene <i>TXN1</i>, but not the <i>NQO1</i>, <i>GPX1</i>, and <i>HMOX1</i> genes, the increased expression of which can lead to the development of resistance to anthracycline antibiotics. Redox-active CPA micelles have great potential for the development of nanoparticles for the transport of anthracycline antibiotics in experimental tumor chemotherapy, and also as promising activators of Nrf2 transcription factor.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the Safety of Anti-Salmonella Disinfectant for Veterinary Use Based on a Cocktail of Bacteriophages 基于噬菌体鸡尾酒的兽用抗沙门氏菌消毒剂的安全性评估
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06225-6
E. R. Zulkarneev, A. I. Laishevtsevtsev, I. A. Kiseleva, O. G. Efimova, T. E. Mizaeva, M. A. Pasivkina, E. S. Zubkova, A. V. Aleshkin, A. V. Karaulov
{"title":"Assessment of the Safety of Anti-Salmonella Disinfectant for Veterinary Use Based on a Cocktail of Bacteriophages","authors":"E. R. Zulkarneev, A. I. Laishevtsevtsev, I. A. Kiseleva, O. G. Efimova, T. E. Mizaeva, M. A. Pasivkina, E. S. Zubkova, A. V. Aleshkin, A. V. Karaulov","doi":"10.1007/s10517-024-06225-6","DOIUrl":"https://doi.org/10.1007/s10517-024-06225-6","url":null,"abstract":"<p>The toxicity and safety of a veterinary anti-salmonella disinfectant based on three highly virulent bacteriophage strains (titers 10<sup>10</sup> PFU/ml) were studied. Acute, chronic, and inhalation toxicity, as well as local irritancy of the disinfectant were evaluated on outbred white mice CD1 (<i>n</i>=65), Soviet chinchilla rabbits (<i>n</i>=20), and rats (<i>n</i>=20). No toxic effects of the disinfectant was observed after its intraperitoneal or intragastric administration to mice and intragastric administration to rats; in rabbits, application on the skin and eyes produced no local irritation effect. Inhalation of 10% of the disinfectant did not cause any pathologies in mice. Thus, the tests confirmed the high level of safety of the disinfectant based on a mixture of bacteriophages for use as an additional specific disinfection agent against <i>Salmonella</i> in veterinary and livestock facilities.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Comparative Study of a Mouse Model of Airway Inflammation and Remodeling Induced by Exosomes Derived from Bone Marrow Mesenchymal Stem Cells 骨髓间充质干细胞外泌体诱导气道炎症和重塑小鼠模型的开发与比较研究
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-16 DOI: 10.1007/s10517-024-06221-w
B. Yao, J. Liu, J. Xie, Z. Li, Y. Luo, M. Wang
{"title":"Development and Comparative Study of a Mouse Model of Airway Inflammation and Remodeling Induced by Exosomes Derived from Bone Marrow Mesenchymal Stem Cells","authors":"B. Yao, J. Liu, J. Xie, Z. Li, Y. Luo, M. Wang","doi":"10.1007/s10517-024-06221-w","DOIUrl":"https://doi.org/10.1007/s10517-024-06221-w","url":null,"abstract":"<p>We developed a model of inflammation and airway remodeling in C57 mice provoked by exosomes derived from bone marrow mesenchymal stem cells infected by respiratory syncytial virus (RSV). The mean size of control and infected exosomes <i>in vitro</i> were 167.9 and 118.5 nm, respectively. After induction of modeled pathology, the severity of airway inflammation and its remodeling were analyzed by histopathological methods. In addition, the blood levels of inflammatory factors IL-10, IL-17, transforming growth factor-β (TGF-β), and TNFα were assayed; in the lung tissues, the expression levels of MMP-2, MMP-9, α-smooth muscle actin (α-SMA), and TGF-β were measured. In the developed model, the effects of RSV-induced and non-induced exosomes were compared with those of inactivated and non-inactivated RSV. Intranasal administration of RSV-induced exosomes decreased the levels of serum inflammatory factors IL-10 and IL-17 and increased the expression of serum proinflammatory cytokine TNFα. Increased levels of MMP-2, MMP-9, and α-SMA, enhanced expression of TGF-β in the lung tissue, and pathological staining of the lung tissues indicated infiltration with inflammatory cells and luminal constriction. Thus, RSV-induced exosomes can provoke airway inflammation and remodeling in mice similar to RSV, while non-induced exosomes cannot produce such alterations.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological Changes in Tissue When Using Polypropylene Implants with Adsorbed Multipotent Stromal Cells in Experiment 在实验中使用吸附了多能基质细胞的聚丙烯植入物时组织形态的变化
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06220-x
I. V. Maiborodin, T. V. Mikheeva, B. V. Sheplev, G. Yu. Yarin, N. V. Onoprienko, V. I. Maiborodina
{"title":"Morphological Changes in Tissue When Using Polypropylene Implants with Adsorbed Multipotent Stromal Cells in Experiment","authors":"I. V. Maiborodin, T. V. Mikheeva, B. V. Sheplev, G. Yu. Yarin, N. V. Onoprienko, V. I. Maiborodina","doi":"10.1007/s10517-024-06220-x","DOIUrl":"https://doi.org/10.1007/s10517-024-06220-x","url":null,"abstract":"<p>The subcutaneous tissue of rats after implantation of polypropylene materials with adsorbed bone marrow-derived mesenchymal multipotent stromal cells (MMSCs) was studied using light microscopy. Inflammation in response to implantation was mild, and the foreign material was encapsulated into a thin strip of dense fibrous connective tissue with multinucleated macrophages. By 1 year after introduction of the monofilament and 6 and 12 months after implantation of the mesh product, some threads were deformed, broken, and had sharp edges. Small fragments of foreign material appeared in the adjacent tissues surrounded by their own relatively thick acellular capsule. As a result of preliminary adsorption of MMSCs on polypropylene, the thickness of the connective tissue capsule decreased, its vascularization increased, and the severity of inflammatory infiltration decreased. However, all effects of MMSCs adsorption in rats were transient and disappeared within 1 week.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Significance of the Expression of Long Non-Coding RNA LINC01508 in Non-Small Cell Lung Cancer 长非编码 RNA LINC01508 在非小细胞肺癌中的表达的临床意义
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06213-w
O. V. Kovaleva, P. A. Podlesnaya, V. V. Mochalnikova, N. E. Kushlinskii, A. N. Gratchev
{"title":"Clinical Significance of the Expression of Long Non-Coding RNA LINC01508 in Non-Small Cell Lung Cancer","authors":"O. V. Kovaleva, P. A. Podlesnaya, V. V. Mochalnikova, N. E. Kushlinskii, A. N. Gratchev","doi":"10.1007/s10517-024-06213-w","DOIUrl":"https://doi.org/10.1007/s10517-024-06213-w","url":null,"abstract":"<p>The expression of long non-coding RNA (lncRNA) LINC01508 was studied in tumor samples from patients with non-small cell lung cancer (NSCLC), and its clinical significance was evaluated. The expression of LINC01508 lncRNA was measured in 16 pairs of NSCLC samples and its association with clinical and morphological features of the disease and prognosis analyzed. A comparative analysis showed a significant decrease in the expression of LINC01508 in tumor lung tissue in comparison with the surrounding normal lung tissue. The informativity of the diagnostic method was tested using ROC curve analysis and calculation of the area under the curve (AUC) for LINC01508 in NSCLC patients, which showed an AUC of 0.875 (<i>p</i>=0.001). No significant associations of LINC01508 expression level with clinical and morphological characteristics of the disease were found. The analysis of prognostic significance showed that high expression of LINC01508 in NSCLC samples was a favorable prognostic factor. Despite the fact that the results did not reach statistical significance (<i>p</i>=0.107), the median survival rate for patients with low LINC01508 expression was 16 months, while for patients with high expression, it was not reached during the follow-up period. We believe that LINC01508 can be a promising independent prognostic marker for NSCLC.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines 在藻酸盐凝胶中获得葡萄球菌单种和二元培养物的新方法，以及以儿茶酚胺为例研究活性化合物对这些培养物的作用
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06217-6
S. V. Mart’yanov, A. V. Gannesen, V. K. Plakunov
{"title":"A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines","authors":"S. V. Mart’yanov, A. V. Gannesen, V. K. Plakunov","doi":"10.1007/s10517-024-06217-6","DOIUrl":"https://doi.org/10.1007/s10517-024-06217-6","url":null,"abstract":"<p>A simple and efficient method for obtaining monospecies and binary <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> cultures in sodium alginate gel matrix mimicking the natural microenvironment of the nasal cavity was proposed. The cultures were used for studying the effect of norepinephrine on monospecies and binary communities of two types of bacteria, <i>S. aureus</i> (invasive strain) and <i>S. epidermis</i> (commensal strain). After 24-h incubation, <i>S. aureus</i> predominated in the binary community, but later it was replaced by <i>S. epidermis</i>. Norepinephrine at higher concentrations accelerated this process without principally changing it. The model can be used to develop more effective complex antimicrobial drugs.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neural and Glial Regulation of Angiogenesis in CNS in Ischemic Stroke 缺血性中风中枢神经系统血管生成的神经和神经胶质调控
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06219-4
L. R. Grinchevskaya, D. I. Salikhova, D. N. Silachev, D. V. Goldshtein
{"title":"Neural and Glial Regulation of Angiogenesis in CNS in Ischemic Stroke","authors":"L. R. Grinchevskaya, D. I. Salikhova, D. N. Silachev, D. V. Goldshtein","doi":"10.1007/s10517-024-06219-4","DOIUrl":"https://doi.org/10.1007/s10517-024-06219-4","url":null,"abstract":"<p>CNS diseases associated with compromised blood supply and/or vascular integrity are one of the leading causes of mortality and disability in adults worldwide and are also among 10 most common causes of death in children. Angiogenesis is an essential element of regeneration processes upon nervous tissue damage and can play a crucial role in neuroprotection. Here we review the features of cerebral vascular regeneration after ischemic stroke, including the complex interactions between endothelial cells and other brain cell types (neural stem cells, astrocytes, microglia, and oligodendrocytes). The mechanisms of reciprocal influence of angiogenesis and neurogenesis, the role of astrocytes in the formation of the blood—brain barrier, and roles of microglia and oligodendrocytes in vascular regeneration are discussed. Understanding the mechanisms of angiogenesis regulation in CNS is of critical importance for the development of new treatments of neurovascular pathologies.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dysmetabolism of Peripheral Blood Monocytes in Type 1 Diabetes Mellitus 1 型糖尿病患者外周血单核细胞的代谢紊乱
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06201-0
V. V. Ivanov, E. E. Buyko, A. A. Ufandeev, K. V. Nevskaya, Ya. S. Slavkina, E. V. Udut, T. V. Saprina, V. V. Udut
{"title":"Dysmetabolism of Peripheral Blood Monocytes in Type 1 Diabetes Mellitus","authors":"V. V. Ivanov, E. E. Buyko, A. A. Ufandeev, K. V. Nevskaya, Ya. S. Slavkina, E. V. Udut, T. V. Saprina, V. V. Udut","doi":"10.1007/s10517-024-06201-0","DOIUrl":"https://doi.org/10.1007/s10517-024-06201-0","url":null,"abstract":"<p>The level of ROS (fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate) and lipid content (fluorescent lipophilic dye Nile Red) in the peripheral blood monocyte fraction from patients with type 1 diabetes mellitus and healthy volunteers were assessed by flow cytofluorimetry. The number of CD36<sup>+</sup> monocytes was assessed using specific antibodies. In patients with type 1 diabetes mellitus, the levels of ROS and intracellular lipids in monocytes and the number of cells expressing CD36 fatty acid translocase were elevated. These results indicate metabolic changes in the peripheral blood cells of patients with carbohydrate metabolism disorders and can be considered as possible prognostic markers for the development of type 1 diabetes mellitus complications.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transduced Olfactory Mucosa Cells Expressing Nerve Growth Factor for the Therapy of Experimental Spinal Cord Cysts 表达神经生长因子的转导嗅粘膜细胞用于治疗实验性脊髓囊肿
IF 0.7 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06222-9
O. V. Stepanova, G. A. Fursa, S. S. Andretsova, E. K. Karsuntseva, V. S. Shishkina, A. V. Chadin, A. D. Voronova, A. S. Semkina, I. V. Reshetov, V. P. Chekhonin
{"title":"Transduced Olfactory Mucosa Cells Expressing Nerve Growth Factor for the Therapy of Experimental Spinal Cord Cysts","authors":"O. V. Stepanova, G. A. Fursa, S. S. Andretsova, E. K. Karsuntseva, V. S. Shishkina, A. V. Chadin, A. D. Voronova, A. S. Semkina, I. V. Reshetov, V. P. Chekhonin","doi":"10.1007/s10517-024-06222-9","DOIUrl":"https://doi.org/10.1007/s10517-024-06222-9","url":null,"abstract":"<p>A new gene-cell construct expressing nerve growth factor (NGF) has been developed. After obtaining engineered adenovectors Ad5-RGD-CAG-NGF and Ad5-RGD-CAG-EGFP, transduction efficiency and transgene expression were studied and multiplicity of infection was determined. The efficacy of transduced human olfactory ensheathing cells expressing NGF in restoring motor activity in rats has been shown in a limited period of time. Improved rat hindlimb mobility and cyst size reduction after gene-cell construct transplantation were more likely due to the cellular component of the construct.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142183650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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