Bulletin of Experimental Biology and Medicine最新文献

Low-Voltage Scanning Electron Microscopy of Extracellular Vesicles Derived from Human Urine, Saliva, Pericardial and Cerebrospinal Fluids. 从人尿、唾液、心包和脑脊液中提取的细胞外囊泡的低压扫描电镜。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-05-08 DOI: 10.1007/s10517-026-06672-3

T D Vavilova, M S Ponomarev, V Yu Mikhailovskii, E A Kondratieva, E N Mikhailov, K A Kondratov

引用次数: 0

Morphometry of Mast Cells in the Tongue Tissues of the White Rats under Stress Conditions. 应激条件下大鼠舌部肥大细胞形态测定。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-29 DOI: 10.1007/s10517-026-06669-y

M Yu Fleishman, A A Salnikov, Yu B Malofey, O Yu Volodina, N Yu Ilyina

引用次数: 0

Cytoprotective Effect of NOP Receptor Blockade in Primary Culture of Pulmonary Fibroblasts. NOP受体阻断对肺成纤维细胞原代培养的细胞保护作用。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-29 DOI: 10.1007/s10517-026-06670-5

E N Sazonova, O G Repa, Yu B Malofey

{"title":"Cytoprotective Effect of NOP Receptor Blockade in Primary Culture of Pulmonary Fibroblasts.","authors":"E N Sazonova, O G Repa, Yu B Malofey","doi":"10.1007/s10517-026-06670-5","DOIUrl":"https://doi.org/10.1007/s10517-026-06670-5","url":null,"abstract":"We investigated the involvement of NOP receptors in the response of the primary fibroblast culture to oxidative stress (OS) and in the mechanisms of the cytoprotective effect of the NALE peptide. OS led to a decrease in the number of cells in monolayers and the absence of mitotic figures, increased the proportion of p53+ cells and the area of the nucleus, and reduced the number of nucleoli. Preliminary incubation with a NOP receptor blocker J-113397 before OS showed its cytoprotective effect: the number of p53+ cells decreased, though still was significantly higher than in the control, the area of the cell nuclei corresponded to the control parameter, and the density of the cell culture increased. Preincubation with the NALE peptide also attenuated the effects of OS in the cell culture. The combined application of the NOP receptor blocker and NALE peptide had a protective effect. Changes in the nucleo-nucleolar apparatus of cells were similar to those observed after incubations with the NALE peptide: a significant increase in the area of nuclei, a decrease in the number of nucleoli, an increase in the total area of nucleoli, and a significant increase in the number of cells in monolayers were recorded. An increase in mitotic activity was found in comparison with the effects of NOP receptor blocker and OS. NOP receptor blocker exhibited a weak anabolic effect: the number of nucleoli and their total area increased. The study demonstrated preservation of the cytoprotective effect of the non-opiate analogue of leu-enkephalin against the background of NOP receptor blockade. Moreover, according to some indicators, there is a synergistic effect of the NALE peptide and the NOP receptor antagonist J-113397.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of an Induced Pluripotent Stem Cell Line from a Duchenne Muscular Dystrophy Patient Carrying the DMD p.Ser429Ter (c.1286C>G) Nonsense Mutation. 杜氏肌萎缩症患者携带DMD p.Ser429Ter （c.1286C>G）无义突变诱导多能干细胞系的生成

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-29 DOI: 10.1007/s10517-026-06671-4

I O Panchuk, O V Grigorieva, E V Kurshakova, S E Nagieva, O A Shchagina, O A Levchenko, V O Pozhitnova, E S Voronina, V Yu Tabakov, S A Smirnikhina, A V Lavrov

引用次数: 0

Tumor Cell Lines as a Biosensor Substrate for Pathological Aging and CNS Tumors. 肿瘤细胞系作为病理衰老和中枢神经系统肿瘤的生物传感器底物。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-28 DOI: 10.1007/s10517-026-06657-2

V V Kudelkina, A M Kosyreva, E A Miroshnichenko

引用次数: 0

The Role of Polymorphism of HLA Class II Genes in Postvaccination Plague Immunity. HLAⅱ类基因多态性在鼠疫疫苗接种后免疫中的作用

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-27 DOI: 10.1007/s10517-026-06656-3

K M Korytov, V I Dubrovina, A B Pyatyidesyatnikova, D D Bryukhova, N O Kiseleva, E A Kuzina, S V Balakhonov

引用次数: 0

Potential of Antioxidant and Cardioprotective Activity of a New Pyridoxine and Fumaric Acid Derivative as an Inductor of Transcription Factor Nrf2. 一种新的吡哆醇和富马酸衍生物作为转录因子Nrf2诱导剂的抗氧化和心脏保护活性潜力。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-27 DOI: 10.1007/s10517-026-06667-0

A A Balakina, A D Podgurskaya, V A Mumyatova, T S Stupina, T R Prikhodchenko, O S Suvorova, S V Blokhina, S Ya Gadomsky, D V Mishchenko

{"title":"Potential of Antioxidant and Cardioprotective Activity of a New Pyridoxine and Fumaric Acid Derivative as an Inductor of Transcription Factor Nrf2.","authors":"A A Balakina, A D Podgurskaya, V A Mumyatova, T S Stupina, T R Prikhodchenko, O S Suvorova, S V Blokhina, S Ya Gadomsky, D V Mishchenko","doi":"10.1007/s10517-026-06667-0","DOIUrl":"https://doi.org/10.1007/s10517-026-06667-0","url":null,"abstract":"The aim of the study was to evaluate the biological activity of a new type of cardioprotectors, activators of Nrf2, based on pyridoxine and fumaric acid derivatives in vitro and in vivo. It was found that di(3-hydroxy-4,5-bis(hydroxymethyl)-2-methylpyridinium) fumarate is an effective activator of Nrf2 transcription factor and exhibits cytoprotective and antioxidant activity in vitro on a cardiomyocyte model. In addition, this compound reduces the cardiotoxic effect of doxorubicin in vivo by suppressing the development of oxidative processes and activating the Nrf2-dependent antioxidant response in the heart. The studied pyridoxine and fumaric acid derivative has high potential as a candidate medication for reducing the side effects of anthracycline antibiotics, as well as for protecting heart cells in various cardiovascular diseases and age-related changes. The revealed effects are of great importance for the development of approaches to designing a new type of cardioprotective compounds, activators of the transcription factor Nrf2.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Amygdalin, 6-Shogaol, and Resveratrol on Inhibition of Differentiation of Monocyte-Derived M4 Macrophages: In Silico and In Vitro Studies. 苦杏仁苷、6-Shogaol和白藜芦醇对单核细胞来源的M4巨噬细胞分化的抑制作用：计算机和体外研究。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-27 DOI: 10.1007/s10517-026-06654-5

Mahdaleny, Arleni Bustami, Fadilah, Febriana Catur Iswanti

引用次数: 0

SNP-Based Identification of Mycobacterium tuberculosis Strains using TaqMan PCR. 基于snp的结核分枝杆菌TaqMan PCR鉴定

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-27 DOI: 10.1007/s10517-026-06655-4

S N Zhdanova, I G Kondratov, O B Ogarkov, S V Kulinich, A V Nesterenko, S Y Oteva, T A Kukhtina, M E Koshcheyev, S N Shugaeva, V V Sinkov, L V Rychkova, L I Kolesnikova

{"title":"SNP-Based Identification of Mycobacterium tuberculosis Strains using TaqMan PCR.","authors":"S N Zhdanova, I G Kondratov, O B Ogarkov, S V Kulinich, A V Nesterenko, S Y Oteva, T A Kukhtina, M E Koshcheyev, S N Shugaeva, V V Sinkov, L V Rychkova, L I Kolesnikova","doi":"10.1007/s10517-026-06655-4","DOIUrl":"https://doi.org/10.1007/s10517-026-06655-4","url":null,"abstract":"Within the WHO framework of genomic epidemiological surveillance of tuberculosis, we have developed a panel of primers and TaqMan probes for PCR-based identification of SNPs in major epidemiological genotypes of Mycobacterium tuberculosis responsible for the spread of MDR/XDR strains in North Eurasia. Validation was performed on a dataset of 590 randomly selected strains circulating in the Krasnoyarsk Krai and Irkutsk region in 2021-2025. We identified 308 and 164 Beijing (L2) strains in the Krasnoyarsk and Irkutsk regions, respectively. The identified Beijing (L2) genotypes included B0/W148 (19 and 76); Central Asian (85 and 47) and its Central Asian Outbreak subtype (12 and 25); and orphan L2 genotypes (14 and 16). We also revealed 88 and 30 Euro-American (L4) strains in the Krasnoyarsk Krai and Irkutsk region, respectively. The identified L4 genotypes included Haarlem (9 and 6); LAM (35 and 7); Ural (19 and 5); S (2 and 0); and orphan L4 genotypes (15 and 12). We developed an approach employing two PCR to discriminate the principal M. tuberculosis genotype set into L2 and L4 lineages covering over 99% of all tuberculosis strains circulating in Russian Federation and other North Eurasian countries. Within the proposed framework, the required and sufficient number of PCR for genotyping and in-depth subtyping does not exceed eight. The feasibility of developing tests for further subtyping of epidemiological M. tuberculosis genotypes is discussed.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Transplantation of Preparations Based on Fibrin Hydrogel and Olfactory Ensheathing Cells for Experimental Therapy of Spinal Cord Injuries. 纤维蛋白水凝胶制剂与嗅鞘细胞移植实验性治疗脊髓损伤的研究。

IF 0.6 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-25 DOI: 10.1007/s10517-026-06668-z

G A Fursa, A I Mukhina, A V Chadin, T V Kuznetsova, A D Voronova, E K Karsuntseva, V S Shishkina, A A Stepanenko, I V Reshetov, O V Stepanova, V P Chekhonin

引用次数: 0