Bulletin of Experimental Biology and Medicine最新文献

{"title":"The Influence of Autophagy-Modulating Drugs on the Expression of Markers Associated with Cancer-Associated Fibroblasts and Epithelial-Mesenchymal Transition in a Mouse Model of Skin Melanoma.","authors":"Iu S Taskaeva, A Yu Shatruk, N P Bgatova","doi":"10.1007/s10517-025-06414-x","DOIUrl":"10.1007/s10517-025-06414-x","url":null,"abstract":"<p><p>The expression of cancer-associated fibroblast (CAF) and epithelial-mesenchymal transition marker proteins was assessed in tumor samples of B16 skin melanoma-bearing mice after chloroquine or lithium administration. Chloroquine increased the expression of CAF markers (αSMA and FAP). Hence, chloroquine can contribute to the activation of individual CAF populations in melanoma. In addition, a decrease in the expression of the epithelial marker E-cadherin and an increase in the expression of the mesenchymal marker vimentin were observed after chloroquine administration, which may indicate activation of epithelial-mesenchymal transition processes in the tumor.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"769-773"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Increased False-Positive ELISA Results for HIV in the Blood of Donors Infected with SARS-CoV-2.","authors":"H Lin, Z Wang, Q Ren, Z Cai, F Ge, W Yuan, T Liao, Y Wu, R Peng, Y Gu, Y Kuang","doi":"10.1007/s10517-025-06411-0","DOIUrl":"10.1007/s10517-025-06411-0","url":null,"abstract":"<p><p>The study examined the effect of IgG antibodies against SARS-CoV-2 virus in the blood of donors after COVID-19 on objectivity of HIV detection in the blood samples with two ELISA variants employing reagents A or B in order to select reliable kits to minimize the false-positive HIV results. Retrospectively, the quality control data of two ELISA kits with reagents A or B developed to detect HIV were analyzed using the control sera together with the results of donor blood tested with both kits, which were collected in the periods from January to December 2022 (n = 55,528) and from January to March 2023 (n = 13,837). From the blood samples obtained in 2023, all the specimens with false-positive HIV results obtained with reagent A were selected (n = 126) together with randomly taken samples of this pool with negative HIV results (n = 345). All selected samples were tested for IgG antibodies against SARS-CoV-2 virus, thereupon the correlation analysis was performed between the samples containing SARS-CoV-2 and those with HIV detected by ELISA reagent A. The data on reactivity of IgG antibodies against SARS-CoV-2 significantly correlated with the false-positive ELISA results for HIV obtained with reagent A (r = 0.451, p < 0.01). In 2023, the share of false-positive ELISA results for HIV obtained with reagent A was 0.91% (126/13,837), which was 6.06-fold greater than the corresponding value in 2022 (0.133% (74/55,528); χ² = 230.38, p < 0.01). This data echoed the SARS-CoV-2 epidemic resulting in the appearance of antibodies against the virus in the donor blood. At the same time, the number of false-positive ELISA results for HIV obtained with reagent B in 2022 (0.014% (8/55,521)) and in 2023 (0.036% (5/13,837)) were essentially smaller than those obtained with reagent A, and the results obtained with the test kit B did not significantly differ from each other for both time periods. Thus, IgG antibodies against SARS-CoV-2 in the blood of donors after COVID-19 can affect the results of HIV detection by some ELISA reagents and consequently elevate the number of false-positive analyses.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"755-759"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the Effectiveness of Bacteriophage BF1354 in the Decontamination of Chicken Meat Artificially Contaminated with an Antibiotic-Resistant Strain of Salmonella.","authors":"E R Zulkarneev, A I Laishevtsev, I A Kiseleva, O G Efimova, A V Aleshkin","doi":"10.1007/s10517-025-06409-8","DOIUrl":"10.1007/s10517-025-06409-8","url":null,"abstract":"<p><p>Salmonella, the agent causing salmonellosis, is one of the most common food pathogens in the world. Bacteriophages are now considered as the most effective and environmentally friendly method to control pathogens in various food products. We evaluated the efficacy of Salmonella phage BF1354 in decontamination of chicken fillet samples artificially contaminated with antibiotic-resistant Salmonella. Sterile chicken fillet samples (5 × 5 cm²) were artificially contaminated with Salmonella Enteritidis and then treated with bacteriophage at a concentration of 10⁹ PFU/ml. The samples were stored at 4°C for 10 days. The number of Salmonella on days 1, 6, and 10 of the experiment decreased by 2.1, 2, and 0.8 log(CFU/g), respectively, in comparison with the control. The results demonstrate the efficacy of bacteriophage BF1354 in reducing the number of Salmonella cells in chicken fillets at refrigerated storage temperatures.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"744-749"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Behavioral Disorders in Laboratory Animals Using a Model of Unpredictable Chronic Mild Stress.","authors":"V M Pavlov, V A Palikov, A Yu Fedotova, M V Shinelev, I A Pakhomova, M S Kazakova, S I Baidarova, I A Dyachenko","doi":"10.1007/s10517-025-06419-6","DOIUrl":"10.1007/s10517-025-06419-6","url":null,"abstract":"<p><p>Using the developed model of unpredictable mild chronic stress in rats, the time to the development of an anxious condition without severe depressive disorders were determined. A combination of stressors to achieve this condition is proposed: individual housing, food and water deprivation, forced swimming in cold (4°C) water, keeping on wet bedding and without bedding, under bright lighting, and at 45°C, light/dark cycle alterations, and 45° cage tilting. The condition of the animals was analyzed by changes in body weight, fructose consumption, coat state, as well as by the parameters of open field and forced swimming tests and nest building. These methods can be used in the study and search for new drugs for the treatment and prevention of anxiety- and depression-like conditions.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"794-797"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonogenic Capacity and Migration Potential of A549 and HeLa Cell Lines Survived after Acute X-Ray Irradiation at a Dose of 15 Gy.","authors":"A A Tsishnatti, S M Rodneva, Yu A Fedotov, V D Gurev, V N Belyaev, D V Guryev","doi":"10.1007/s10517-025-06413-y","DOIUrl":"10.1007/s10517-025-06413-y","url":null,"abstract":"<p><p>We obtained A549 and HeLa cell lines that survived and showed stable growth after acute X-ray irradiation at a dose of 15 Gy (hereinafter referred to as A549RR and HeLaRR). Comparative studies of the proliferative activity of original (A549, HeLa) and survived (A549RR, HeLaRR) cells and their sensitivity to additional X-ray irradiation at doses of 2, 4, and 6 Gy by the clonogenic capacity and migration potential (scratch-wound test) were carried out. It was found that the doubling time of the cell population for the A549RR and HeLaRR lines was significantly lower than for the original non-irradiated cells without additional exposure. A549RR and HeLaRR cell lines demonstrated higher resistance to additional X-ray irradiation in terms of clonogenic ability. Migration potential, as one of the critical processes during tumor metastasis, appeared to be significantly higher for A549RR cells up to 24 h after irradiation at a dose of 2 Gy and up to 48 h after irradiation at a dose of 4 Gy. Migration of HeLaRR cells was significantly higher without additional exposure and after irradiation at doses of 2 and 4 Gy, while after irradiation at a dose of 6 Gy, migration activity of HeLaRR cells was significantly suppressed compared to original cells.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"764-768"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Model of Partially Recovered Blood Loss in Awake Rats.","authors":"A M Ismailova, M S Severyukhina, V A Palikov, N A Borozdina, S G Semushina, A V Cholina, O N Khokhlova, D I Rzhevsky, G A Slascheva, I A Dyachenko, A N Murashev","doi":"10.1007/s10517-025-06416-9","DOIUrl":"10.1007/s10517-025-06416-9","url":null,"abstract":"<p><p>We propose a new model of blood loss in awake rats with volume loss up to 40% of total blood volume in the phase of hemorrhagic shock and partial blood replacement up to final blood loss up to 30% of total blood volume. During blood withdrawal, the rats showed manifestation of baroreflex, increased blood levels of glucose and lactate, and decreased concentration of oxyhemoglobin. Partial blood volume compensation was followed by recovery of hemodynamics the levels of glucose and oxyhemoglobin. Thus, taking into account the awake status of rats and compensatory mechanisms, our model of blood loss with a fixed volume of blood drawing and its partial compensation is a relevant model for studying the efficacy of blood substitutes.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"778-781"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Immunogenic Efficacy of Inactivated Monovalent Vaccines against Newcastle Disease in the Republic of Kazakhstan.","authors":"N Zh Akmyrzayev, N N Assanzhanova, Zh K Kydyrbayev, Sh Zh Ryskeldinova, A T Zhunushov, Ye M Kozhamkulov, A M Mailybayeva, A M Sagymbayeva, Ye T Myrzakhmetov","doi":"10.1007/s10517-025-06408-9","DOIUrl":"10.1007/s10517-025-06408-9","url":null,"abstract":"<p><p>The effectiveness of the inactivated vaccines developed by us against the Newcastle disease virus was studied in chickens after infection with a highly virulent Alatau-98 strain. The vaccines in the form of a stable water-oil emulsion were prepared using Montanide ISA 70 VG adjuvant. The immunogenicity of the vaccines was checked 7, 14, 21, and 28 days after administration. The effectiveness was determined by the ability of the vaccine preparations to protect against the disease and by mortality after infection with a highly virulent Alatau-98 strain. Inactivated vaccines developed by us have shown promising results in protecting birds from Newcastle disease and can significantly improve disease control in domestic poultry.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"739-743"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Proteolytic Activity in Esophageal Cancer.","authors":"E I Goufman, N B Tikhonova, K B Gershkovich, A P Aleksankin, A A Stepanov, N V Nizyaeva, V V Bochkov, L M Mikhaleva, O V Kovaleva, I S Stilidi, N E Kushlinskii","doi":"10.1007/s10517-025-06415-w","DOIUrl":"10.1007/s10517-025-06415-w","url":null,"abstract":"<p><p>The growth of malignant tumors is accompanied by an increase in plasmin activity, one of the key enzymes in oncogenesis. IgG proteolysis by plasmin yields fragments with exposed C-terminal lysine residues that can bind to plasmin heavy chain. Highly purified plasminogen (plasmin precursor) and heavy chains containing lysine-binding sites were isolated. The formation of IgG fragments as a result of their proteolysis by plasmin in solution was shown. Using ELISA with immobilized heavy chains, we found increased concentration of IgG fragments in the sera of patients with esophageal cancer in comparison with healthy donors. The test had high sensitivity (91%) and specificity (85%).</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"774-777"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of Frequency Dependence of Selectivity and Sensitivity of Mammalian Otolith Membranes during Electrical Stimulation of the Vestibular Nerves.","authors":"V V Udut, V P Demkin, S V Melnichuk, T V Rudenko, M V Svetlik, M D Akinina, O V Demkin, L V Smaglii, V M Alifirova, O V Grebenuk","doi":"10.1007/s10517-025-06400-3","DOIUrl":"10.1007/s10517-025-06400-3","url":null,"abstract":"<p><p>A numerical experiment was conducted to determine the spectral sensitivity and selectivity of the cellular components of the utricle and saccule of a laboratory animal (rat) to a periodic electrical stimulation. Equivalent electrical circuit of otolith replacement was used. Its parameters were determined from the anatomical structure and from the measured specific characteristics of cellular elements taking into account the changing ionic conductivity. It was found that the frequency dependence of the impedance and the spectral sensitivity of the cell elements for the utricle and saccule have a resonant nature. The obtained data about the mechanisms of electrical stimulation of hair cells, their spectral sensitivity, and frequency selectivity can serve as the basis for choosing the optimal mode of electrical stimulation in a vestibular implant.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"697-701"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective Effects of Thymogen Analogues in Hydrazine Hepatopathy in Rats.","authors":"A A Chulanova, A M Smakhtina, G S Mal, I I Bobyntsev, M Yu Smakhtin, E S Mishina, P M Kopeykin, E G Bogomolova","doi":"10.1007/s10517-025-06405-y","DOIUrl":"10.1007/s10517-025-06405-y","url":null,"abstract":"<p><p>We studied the reparative and antioxidant effects of thymogen (10 and 100 μg/kg) and its modifications in equimolar doses (12 and 120 μg/kg) obtained by attaching the amino acid D-alanine (D-Ala) to the N- or C-terminus of the peptide molecule. A single intraperitoneal injection of hydrazine hydrochloride caused a decrease in catalase activity and an increase in molondialdehyde (MDA) concentration. Administration of peptides in lower doses inhibited LPO and stimulated reparative regeneration of hepatocytes. Thymogen analogue with D-Ala at the C-terminus was most effective. Increasing the doses of thymogen (100 μg/kg) and its analogues (120 μg/kg) did not lead to further increase in their hepatoprotective activity.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"722-725"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}