V S Ovechkina, P S Suvorova, S K Andrianova, V V Belousov, A A Mozhaev
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Generation of a HEK293 Cell Line with Stable Expression of Molecular Tools for Thermogenetic Activation.
In modern biotechnological and biomedical research, it is often necessary to activate mammalian cells to obtain a specific response, such as changes in membrane potential or generation of action potential in excitable cells and tissues. Thermogenetics provides a rapid and controlled cellular response and is a promising tool for such tasks. In order to explore the potential of thermogenetic approaches, we generated the HEK293 cell line stably expressing the thermosensitive human TRPV1 ion channel. This cell line exhibited an increase in intracellular Ca2+ ion concentration upon exposure to temperatures above 39°C and/or addition of capsaicin. The developed model can be used to further study the functional characteristics of exogenously expressed TRPV1 channels in mammalian cells.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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