Bulletin of Experimental Biology and Medicine最新文献_第10页

STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer. STRAP 基因敲除抑制非小细胞肺癌的迁移和生长

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06267-w

X Chen, C Xu, Y Yang, L Li, R Hong

{"title":"STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer.","authors":"X Chen, C Xu, Y Yang, L Li, R Hong","doi":"10.1007/s10517-024-06267-w","DOIUrl":"10.1007/s10517-024-06267-w","url":null,"abstract":"Serine-threonine kinase receptor-associated protein (STRAP) regulates cell proliferation and apoptosis by binding to many target proteins and plays an important regulatory role in tumor development. We studied the effects of STRAP on non-small cell lung cancer (NSCLC) in vivo and in vitro in order to elucidate possible mechanisms underlying the regulatory effects of this protein. The levels of STRAP in NSCLC tissues and cells were determined by quantitative reverse transcription PCR, immunohistochemical staining, and Western blotting. In in vitro experiments, A549 and HCC827 cells were transfected with small interfering RNA (siRNA) to knockdown STRAP (si-STRAP) or with negative control sequence; cell migration and invasion were detected by scratch and Transwell assays, respectively. The expression levels of X-linked inhibitor of apoptosis protein (XIAP), caspase-3, and caspase-9 were determined by Western blotting. In addition, we analyzed changes of tumor volume in a nude mouse NSCLC model. STRAP was highly expressed in NSCLC tissues and cells, but its expression was significantly suppressed in A549 and HCC827 cells transfected with si-STRAP. STRAP knockdown resulted in a significant inhibition of migration and invasion of A549 and HCC827 cells. It also significantly reduced the expression of XIAP and elevated expression of caspase-3 and caspase-9. In nude mice with tumor originated from transplanted A549 cells, inhibition of STRAP expression retarded the tumor growth. Overall, these findings indicate that STRAP is overexpressed in NSCLC, while knockdown of STRAP gene inhibits the growth of NSCLC.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"780-786"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Protocatechuic Aldehyde on Cerebral Ischemia/Reperfusion Injury in Rats through Blood-Brain Barrier Protection. 原儿茶醛通过血脑屏障保护作用对大鼠脑缺血再灌注损伤的保护作用

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06264-z

F He, J Feng, H Sun, Y Xu, H Yan, X Song, Y Wang, X Li, Q Lin

{"title":"Protective Effect of Protocatechuic Aldehyde on Cerebral Ischemia/Reperfusion Injury in Rats through Blood-Brain Barrier Protection.","authors":"F He, J Feng, H Sun, Y Xu, H Yan, X Song, Y Wang, X Li, Q Lin","doi":"10.1007/s10517-024-06264-z","DOIUrl":"10.1007/s10517-024-06264-z","url":null,"abstract":"Cerebral ischemia can lead to destruction of the blood-brain barrier (BBB), the main cause of cerebral edema and cerebral infarction. BBB damage is also one of the key factors affecting the result of drug therapy. We studied the protective effect of 5-day pretreatment with protocatechuic aldehyde (PAL) at doses of 10 and 20 mg/kg on BBB function and structure after middle cerebral artery occlusion/reperfusion (MCAO/R) in rats. The infarct volume, behavioral neurological deficit score, and Evans blue content in the brain were estimated. We also evaluated the content of nitric oxide (NO) and activities of inducible and neuronal NO synthases. Expression of aquaporin-4 (AQP-4), occludin, claudin-5, and MMP-3 in the brain tissues was estimated by Western blotting. The BBB ultrastructure was analyzed under an electron microscope. We revealed that PAL at both used doses significantly reduced the neurological deficit score, brain infarct volume, and Evans blue extravasation. Electron microscopy showed that PAL significantly improved the ultrastructure of BBB and alleviated its injury. Pretreatment with PAL increased expression of occludin and claudin-5 and reduced expression of AQP-4 and MMP-3. At the same time, the release of NO and activities of NO synthases were notably inhibited. Our results suggest that PAL can be a promising compound to attenuate cerebral ischemia resulting from occlusion/reperfusion injury via BBB protection.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"763-769"},"PeriodicalIF":0.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Involvement of GABA in the Modulation of the Rhythm of Electrical Activity in the Small Intestine during Food Deprivation. GABA参与食物缺乏时小肠电活动节律的调节。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06253-2

N S Tropskaya, Yu V Gurman

引用次数: 0

Approbation of a Homologous Model of the Antitumor Vaccine Based on Mature Mouse Dendritic Cells to Study the Biodistribution of the Cell Product 批准基于成熟小鼠树突状细胞的抗肿瘤疫苗同源模型，以研究细胞产品的生物分布情况

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06226-5

T. L. Nekhaeva, I. D. Laskov, E. I. Fedoros, A. B. Danilova, M. N. Yurova, M. L. Tyndyk, E. D. Ermakova, N. V. Emelyanova, N. A. Efremova, A. V. Grigorevskaya, M. A. Nekrasova, I. A. Baldueva

引用次数: 0

Influence of Redox-Active Chitosan-Polyaminoxyl Micelles Loaded with Daunorubicin on Activity of Nrf2 Transcription Factor 载入多柔比星的具有氧化还原活性的壳聚糖-多氨氧胶束对 Nrf2 转录因子活性的影响

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06224-7

A. A. Balakina, V. I. Amozova, V. D. Sen’

{"title":"Influence of Redox-Active Chitosan-Polyaminoxyl Micelles Loaded with Daunorubicin on Activity of Nrf2 Transcription Factor","authors":"A. A. Balakina, V. I. Amozova, V. D. Sen’","doi":"10.1007/s10517-024-06224-7","DOIUrl":"https://doi.org/10.1007/s10517-024-06224-7","url":null,"abstract":"A new system for delivery of anthracycline antibiotics based on chitosan-polyaminoxyls (CPA) was studied in a model of non-tumor (human embryonic mesenchymal stem cells) and tumor cells (human hepatocellular carcinoma) in vitro. The presence of CPA micelles considerably suppresses daunorubicin-induced ROS generation in normal cells without affecting this process in tumor cells. CPA micelles do not reduce the cytotoxic effect of daunorubicin and do not prevent its accumulation in cells. The use of CPA significantly increases accumulation of Nrf2 transcription factor in the nuclei of both normal and tumor cells in comparison with free daunorubicin. Increased nuclear translocation of Nrf2 leads to a significant increase in the expression of its target gene TXN1, but not the NQO1, GPX1, and HMOX1 genes, the increased expression of which can lead to the development of resistance to anthracycline antibiotics. Redox-active CPA micelles have great potential for the development of nanoparticles for the transport of anthracycline antibiotics in experimental tumor chemotherapy, and also as promising activators of Nrf2 transcription factor.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"22 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the Safety of Anti-Salmonella Disinfectant for Veterinary Use Based on a Cocktail of Bacteriophages 基于噬菌体鸡尾酒的兽用抗沙门氏菌消毒剂的安全性评估

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI: 10.1007/s10517-024-06225-6

E. R. Zulkarneev, A. I. Laishevtsevtsev, I. A. Kiseleva, O. G. Efimova, T. E. Mizaeva, M. A. Pasivkina, E. S. Zubkova, A. V. Aleshkin, A. V. Karaulov

引用次数: 0

Development and Comparative Study of a Mouse Model of Airway Inflammation and Remodeling Induced by Exosomes Derived from Bone Marrow Mesenchymal Stem Cells 骨髓间充质干细胞外泌体诱导气道炎症和重塑小鼠模型的开发与比较研究

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-16 DOI: 10.1007/s10517-024-06221-w

B. Yao, J. Liu, J. Xie, Z. Li, Y. Luo, M. Wang

{"title":"Development and Comparative Study of a Mouse Model of Airway Inflammation and Remodeling Induced by Exosomes Derived from Bone Marrow Mesenchymal Stem Cells","authors":"B. Yao, J. Liu, J. Xie, Z. Li, Y. Luo, M. Wang","doi":"10.1007/s10517-024-06221-w","DOIUrl":"https://doi.org/10.1007/s10517-024-06221-w","url":null,"abstract":"We developed a model of inflammation and airway remodeling in C57 mice provoked by exosomes derived from bone marrow mesenchymal stem cells infected by respiratory syncytial virus (RSV). The mean size of control and infected exosomes in vitro were 167.9 and 118.5 nm, respectively. After induction of modeled pathology, the severity of airway inflammation and its remodeling were analyzed by histopathological methods. In addition, the blood levels of inflammatory factors IL-10, IL-17, transforming growth factor-β (TGF-β), and TNFα were assayed; in the lung tissues, the expression levels of MMP-2, MMP-9, α-smooth muscle actin (α-SMA), and TGF-β were measured. In the developed model, the effects of RSV-induced and non-induced exosomes were compared with those of inactivated and non-inactivated RSV. Intranasal administration of RSV-induced exosomes decreased the levels of serum inflammatory factors IL-10 and IL-17 and increased the expression of serum proinflammatory cytokine TNFα. Increased levels of MMP-2, MMP-9, and α-SMA, enhanced expression of TGF-β in the lung tissue, and pathological staining of the lung tissues indicated infiltration with inflammatory cells and luminal constriction. Thus, RSV-induced exosomes can provoke airway inflammation and remodeling in mice similar to RSV, while non-induced exosomes cannot produce such alterations.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"31 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Changes in Tissue When Using Polypropylene Implants with Adsorbed Multipotent Stromal Cells in Experiment 在实验中使用吸附了多能基质细胞的聚丙烯植入物时组织形态的变化

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06220-x

I. V. Maiborodin, T. V. Mikheeva, B. V. Sheplev, G. Yu. Yarin, N. V. Onoprienko, V. I. Maiborodina

引用次数: 0

A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines 在藻酸盐凝胶中获得葡萄球菌单种和二元培养物的新方法，以及以儿茶酚胺为例研究活性化合物对这些培养物的作用

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06217-6

S. V. Mart’yanov, A. V. Gannesen, V. K. Plakunov

引用次数: 0

Neural and Glial Regulation of Angiogenesis in CNS in Ischemic Stroke 缺血性中风中枢神经系统血管生成的神经和神经胶质调控

IF 0.7 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI: 10.1007/s10517-024-06219-4

L. R. Grinchevskaya, D. I. Salikhova, D. N. Silachev, D. V. Goldshtein

引用次数: 0