Bulletin of Experimental Biology and Medicine最新文献

{"title":"Application of Raman Spectroscopy for Selecting Seminal Plasma Donors.","authors":"A S Yakimova, E N Rimskaya, A V Gorevoy, N P Makarova, E A Kalinina","doi":"10.1007/s10517-026-06758-y","DOIUrl":"https://doi.org/10.1007/s10517-026-06758-y","url":null,"abstract":"<p><p>The composition of seminal plasma from men with varying ejaculate parameters was analyzed using Raman spectroscopy. Distinct differences were identified in the Raman spectra of fertile men and infertile patients, particularly in amino acids, phospholipids, and saccharides. A comparative analysis of seminal plasma biomolecules was performed within the group of fertile men who were potential seminal plasma donors. It was found that the seminal plasma from certain fertile donors exhibits a similar spectral profile and has a positive effect on the morphofunctional characteristics of spermatozoa. These findings support the use of Raman spectral signatures for the selection of seminal plasma donors and the refinement of donor selection criteria.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Interferon-Gamma (Ingaron) against Experimental Tuberculosis in BALB/c Mice.","authors":"I A Vasilyeva, A G Samoylova, V V Avdeev, M I Romanova, J R Zyuzya, S I Kayukova","doi":"10.1007/s10517-026-06754-2","DOIUrl":"https://doi.org/10.1007/s10517-026-06754-2","url":null,"abstract":"<p><p>The therapeutic activity of an IFN-γ preparation Ingaron, both alone and in combination with isoniazid, was studied in an experimental model of tuberculosis infection in inbred BALB/c mice. The combination of oral isoniazid and subcutaneous IFN-γ administration demonstrated high efficacy, which was confirmed by microbiological and morphological examination of the lungs and spleens, as well as by the organ weight dynamics.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Therapeutic and Biopharmaceutical Properties of a Biadhesive Dental Paste Based on a Complex Immunoglobulin Preparation.","authors":"K E Petrulenko, L R Seifullaeva, V V Shulakov, O O Prosycheva, M S Podporin, V N Tsarev, N B Demina, L A Korol, A V Alyoshkin, V A Alyoshkin, M N Anurova","doi":"10.1007/s10517-026-06753-3","DOIUrl":"https://doi.org/10.1007/s10517-026-06753-3","url":null,"abstract":"<p><p>A topical mucoadhesive dosage form was developed for the treatment of infectious oral cavity diseases, consisting of a paste based on a combination of a complex immunoglobulin preparation (CIP) and interferon alpha-2b. CIP within the final formulation demonstrated robust antimicrobial activity against key oral pathogens (Staphylococcus aureus, Streptococcus intermedius, and Fusobacterium nucleatum). An optimal composition was selected that exhibited optimal mucoadhesion and a mucosal retention time of at least 90 min. The final formulation produced no local irritant effects.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmonic Analysis of Electrical Activity of the Autonomic Nervous System in Dorsal Perirenal Lumbar Regions in Healthy Volunteers and Patients with Arterial Hypertension.","authors":"A D Makarov, A V Prosvirnin, N M Danilov, E G Popov, E E Popov, I Y Gavrilov, E V Limonov, S V Revenko","doi":"10.1007/s10517-026-06756-0","DOIUrl":"https://doi.org/10.1007/s10517-026-06756-0","url":null,"abstract":"<p><p>To non-invasively assess the activity of the autonomic nervous system (ANS) and the pulsatile activity of arteries in the perirenal dorsal lumbar regions, electrical bioimpedance (BI) and biopotentials (BP) were recorded in healthy volunteers and patients with arterial hypertension (AH). Signals were processed using harmonic (Fourier) analysis to determine the effective values (rms) of spectral peaks for BI (MR, THR) and BP (MV, THV) in the Mayer (M) frequency range (0.03-0.2 Hz) and the Traube-Hering (TH) frequency band of a controlled respiration rate (0.3-0.4 Hz), which presumably reflect sympathetic and parasympathetic activity, respectively. The pulsatile activity of arteries was determined by the total effective value (TPrms) of four pulsatile harmonics. The indices of the sympathetic-parasympathetic balance were calculated as the ratios of MR/THR (for BI) and MV/THV (for BP). In healthy participants, the active orthostatic test significantly increased THR, MR, TPrms^-1, and MV, which reflected increased activity of sympathetic (MR, TPrms^-1, MV) and parasympathetic (THR) systems while maintaining overall autonomic balance. The spectral parameters of BI and BP were presented with normalized radar charts (RC). In patients with controlled AH, the median RCs were located within the interquartile range of RC of the healthy group indicating adequate drug therapy. In contrast, individual RCs of some patients with uncontrolled or refractory AH were outside this range, indicating the need to correct drug therapy or feasibility of renal denervation if pharmacotherapy fails. The advanced method enables the assessment of ANS activity in patients with AH of varying severity. It is concluded that harmonic analysis of electrical signals in human perirenal regions is a valuable non-invasive tool for evaluating local activity of ANS under physiological conditions and in AH patients.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotrophic and Neuroprotective Effects of Magnesium Chelidonate and Chelidonic Acid in Cell Culture Experiments.","authors":"S V Kozin, A A Kravtsov, A V Moiseev, A D Rubailo, S V Bobrovnik, E R Vasilevskaya, A I Trofimenko, O M Lyasota","doi":"10.1007/s10517-026-06749-z","DOIUrl":"https://doi.org/10.1007/s10517-026-06749-z","url":null,"abstract":"<p><p>The neuroprotective and neurotrophic properties of magnesium chelidonate and chelidonic acid at concentrations of 0.01 and 0.1 mM were studied. It was found that magnesium chelidonate and chelidonic acid significantly reduced cell death in primary rat cerebellar neuroglial cultures, both in a model of glutamate excitotoxicity and under conditions of oxygen-glucose deprivation. Both substances exerted a stimulatory effect on the neurotrophic processes in chicken embryo dorsal root ganglion cultures. The neuroprotective and neurotrophic effects of the tested compounds were more pronounced at a concentration of 0.01 mM. In our experiments, magnesium salt of chelidonic acid produced more potent neuroprotective and neurotrophic effects on the studied cultures than chelidonic acid.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Peroxynitrite on Endothelial-Mesenchymal Transition Markers in Primary Endothelial Cell Cultures In Vitro.","authors":"E A Strelnikova, R E Kalinin, I A Suchkov, N D Mzhavanadze, Yu V Abalenikhina, N V Korotkova","doi":"10.1007/s10517-026-06748-0","DOIUrl":"https://doi.org/10.1007/s10517-026-06748-0","url":null,"abstract":"<p><p>We studied the effects of the nitrosative stress modulator peroxynitrite on changes in key markers of the endothelial-mesenchymal transition (EndMT) in primary endothelial cell cultures in vitro. Peroxynitrite exposure significantly increased the relative content of endothelial markers (platelet adhesion molecules (CD31) and von Willebrand factor) and reduced the levels of mesenchymal markers (fibronectin and vimentin). These findings suggest that peroxynitrite may inhibit EndMT in vitro.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Features of Thyroid Signaling Disorders in Experimental Pulmonary Arterial Hypertension.","authors":"A A Abramov, A G Bobylev, I M Vikhlyantsev, V L Lakomkin","doi":"10.1007/s10517-026-06745-3","DOIUrl":"https://doi.org/10.1007/s10517-026-06745-3","url":null,"abstract":"<p><p>Pulmonary arterial hypertension (PAH) is characterized by a neurohumoral imbalance. We studied the effect of PAH on transcriptional activity in pulmonary artery preparations. In rats with PAH induced by monocrotaline (60 mg/kg), pulmonary artery preparations were isolated and whole-transcriptome RNA sequencing was performed. The enrichment score of gene collections from the Gene Ontology and KEGG databases was determined. Significant gene sets were grouped into clusters, and genes exhibiting the greatest changes in expression and representation within these clusters were identified. The representation of functional domains in protein sequences was characterized for the key genes. PAH led to a significant increase in the enrichment score of pathways associated with contractile elements and the cytoskeleton of muscle cells (including cardiomyocytes) and a decrease in the score for pathways associated with the synthesis of ribosomal components. Clustering of the pathways revealed changes in gene expression indicative of impaired thyroid signaling.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Effects of Secretory Phospholipase A₂ from Venoms of Gloydius blomhoffii and Gloydius halys.","authors":"M A Solovyeva, L V Galebskaya, M A Galkin, E V Ryumina, G M Berdichevskiy, O S Shemchuk, E V Semenova, A V Ivanova, I M Mikhel, E A Rogozhin, L V Vasina","doi":"10.1007/s10517-026-06750-6","DOIUrl":"https://doi.org/10.1007/s10517-026-06750-6","url":null,"abstract":"<p><p>Gloydius blomhoffii and Gloydius halys venoms contain considerable amounts of group IIA secretory phospholipase (sPLA₂). We studied the cytotoxic effects of some fractions from these venoms on the A549 lung adenocarcinoma cell line. G. blomhoffii venom fraction 16 and G. halys venom fraction 22 exhibited significant cytotoxicity against A549 cells (cell viability 10.7 ± 1.5% and 11.7 ± 2.7%, respectively). The sPLA₂-sensitive melanoma SK-MEL-2 cell line and the sPLA₂ inhibitor varespladib were used for sPLA₂ identification and evaluation of its cytotoxicity in these fractions. Cell viability in the presence of the inhibitor increased by 26.4% (fraction 16) and 13% (fraction 22), which confirms a link between the cytotoxic effect and the enzymatic activity of sPLA₂ in the fractions examined. The two protein components were further identified as acidic phospholipases A₂.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-Induced Changes in Contractile Activity of Rat Aorta and Pulmonary Artery.","authors":"S V Gusakova, V S Gusakova, E A Lebedeva, M A Pshemyskiy, M Kilin, L N Maslov","doi":"10.1007/s10517-026-06747-1","DOIUrl":"https://doi.org/10.1007/s10517-026-06747-1","url":null,"abstract":"<p><p>Immobilization stress (12 and 24 h) simulated in female Wistar rats reduced the amplitude of phenylephrine-induced contraction of isolated aortic and pulmonary artery rings. Acetylcholine-induced, endothelium-dependent vasorelaxation of the aorta and pulmonary artery increased after 12-h stress exposure. Therefore, stress modulates the functional state of these arteries without signs of endothelial injury.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olesoxime Does Not Affect Mitochondrial Dysfunction and Skeletal Muscle Pathology in Dystrophin-Deficient mdx Mice.","authors":"A A Cherepanova, I B Mikheeva, A E Stepanova, K N Popova, A D Igoshkina, V A Sharapov, A S Ustinova, A A Semenova, M V Dubinin","doi":"10.1007/s10517-026-06746-2","DOIUrl":"https://doi.org/10.1007/s10517-026-06746-2","url":null,"abstract":"<p><p>Mitochondrial dysfunction is an important component in the pathogenesis of Duchenne muscular dystrophy (DMD). Although the mitochondrial voltage-dependent anion channel (VDAC) inhibitor olesoxime (TRO19622) has demonstrated efficacy in a severe DMD model, its effects in the classic mdx mouse model with a mild phenotype remain unclear. In this study, we administered olesoxime to 4-week-old mdx mice (20 mg/kg every other day for 4 weeks) and evaluated key parameters of mitochondrial function and muscle pathology. We found that olesoxime exerted no beneficial effects on impaired oxidative phosphorylation, calcium homeostasis, or mitochondrial ultrastructure; it also failed to reduce oxidative (4-HNE) or proteotoxic (calpain activity) stress levels, and did not improve muscle histopathology or functional indicators. These results demonstrate that, unlike in the severe DMD model, olesoxime does not exhibit therapeutic efficacy in the mdx mouse model. Our findings suggest that the effectiveness of mitochondron-targeted therapies based on VDAC inhibitors critically depends on the initial severity of mitochondrial dysfunction, which must be considered during the preclinical evaluation of potential therapeutic agents.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}