Bulletin of Experimental Biology and Medicine最新文献_第2页

Psychopharmacological and Neuroregenerative Effects of a NF-κB Inhibitor under Conditions of Modeled Ethanol-Induced Encephalopathy.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI: 10.1007/s10517-025-06368-0

G N Zyuz'kov, V V Zhdanov, L A Miroshnichenko, T Yu Polyakova, E V Simanina, A V Chaykovskyi, V I Agafonov

引用次数: 0

Bioinformatic Analysis of the Transcriptomes of the Spinal Cord Lumbar Enlargement Cells of Mice under Condition of Hind Limb Unloading and Subsequent Rehabilitation.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-25 DOI: 10.1007/s10517-025-06348-4

A N Lisyukov, M I Markelova, I I Gainullin, E E Bekmukhamedova, V V Valiullin, R R Islamov

引用次数: 0

The Effect of Poloxamer Coating on the Toxicity of Carbon Dots in Glioblastoma and Embryonic Kidney Models In Vitro.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI: 10.1007/s10517-025-06362-6

V V Kudelkina, D U Musaeva, A V Syu, A N Kopylov, A M Kosyreva, A I Alekseeva, O V Makarova, V Yu Timoshenko

{"title":"The Effect of Poloxamer Coating on the Toxicity of Carbon Dots in Glioblastoma and Embryonic Kidney Models In Vitro.","authors":"V V Kudelkina, D U Musaeva, A V Syu, A N Kopylov, A M Kosyreva, A I Alekseeva, O V Makarova, V Yu Timoshenko","doi":"10.1007/s10517-025-06362-6","DOIUrl":"10.1007/s10517-025-06362-6","url":null,"abstract":"Carbon dots (CDs), produced by microwave or hydrothermal synthesis, are low-toxic, carbon-based semiconductor nanoparticles that can be used for imaging of biological objects and antitumor therapy. To impart new functional properties to the CDs for biomedical purposes, they are coated with surfactants, such as poloxamers or pluronics. These are block copolymers of polyoxyethylene and polyoxypropylene. We assessed the cytotoxicity of CDs with and without surfactant coatings in in vitro experiments conducted on mouse glioblastoma M6 and human kidney embryonic HEK293 cell lines. The cell viability evaluated in MTT test and morphological study showed that CDs at concentrations of 0.45 and 0.88 mg/ml without a surfactant coating did not significantly inhibit the growth of HEK293 or M6 cells. However, CDs coated with surfactant (1% poloxamer solution) at a concentration of 0.65 mg/ml significantly reduced the metabolic activity of both cell lines. Thus, the use of a surfactant-coated CDs reduced the metabolic activity of cells. This, combined with their luminescent properties, can be used for both the simultaneous therapy and diagnosis of tumors.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"491-495"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Therapeutic Efficacy of Stromal Cells Isolated from Human Corneal Lenticules for Corneal Repair after Injury in an In Vivo Model.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI: 10.1007/s10517-025-06375-1

M A Surovtseva, N A Bondarenko, K Yu Krasner, I I Kim, E V Chepeleva, D L Cherepanova, D D Dmitriev, A N Trunov, V V Chernykh, O V Poveshchenko

引用次数: 0

Effect of Immunization with Type 5 Group A Streptococcal Antigens and Liposaccharide (LPS) In Vivo and In Vitro on Splenic and Bone Marrow Multipotent Stromal Cells in Mice.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI: 10.1007/s10517-025-06352-8

Yu F Gorskaya, T A Danilova, A I Tukhvatulin, A Sh Dzharullaeva, E N Semenova, L M Vavilova, E V Nagurskaya, V A Bekhalo, A G Minko, V G Nesterenko

{"title":"Effect of Immunization with Type 5 Group A Streptococcal Antigens and Liposaccharide (LPS) In Vivo and In Vitro on Splenic and Bone Marrow Multipotent Stromal Cells in Mice.","authors":"Yu F Gorskaya, T A Danilova, A I Tukhvatulin, A Sh Dzharullaeva, E N Semenova, L M Vavilova, E V Nagurskaya, V A Bekhalo, A G Minko, V G Nesterenko","doi":"10.1007/s10517-025-06352-8","DOIUrl":"10.1007/s10517-025-06352-8","url":null,"abstract":"The number of multipotent stromal cells (MSC) in the splenic transplants of normal CBA mice in recipients immunized with inactivated type 5 group A streptococcal vaccine decreased by 3.5-1.8 times depending on the age of the recipients in comparison with that in the transplants grafted to the control recipients. Such immunization suppressed MSC transplantability, i.e., their potency to construct and maintain specific microenvironment of initial organs. In 24 h after administration of LPS to normal mice in vivo, the MSC content in the spleen and bone marrow increased by 1.8 and 1.6 times, respectively; in immunized mice, these parameters did not increase after LPS injection. The concentration of TNFα in the culture medium of bone marrow from normal mice increased pronouncedly after treatment with LPS both in vivo and in vitro (from 0 to 16.1±2.0 and to 14.2±0.1 pg/ml, respectively), while in the culture medium of bone marrow cells from of immunized mice, this cytokine was not detected after LPS treatment. Probably, immunization of mice with antigens of type 5 group A streptococci and resulting autoimmunity diminish animal sensitivity to LPS, which is reflected by examined parameters of the stromal tissue.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"437-441"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Cultivation with Mesenchymal Stem Cells Does Not Affect the Efficiency of DNA Double-Strand Break Repair in Irradiated Human Non-Small Cell Lung Cancer Cells.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI: 10.1007/s10517-025-06358-2

D V Molodtsova, A N Osipov

引用次数: 0

Morphological and Molecular Peculiarities of Healing of Uterine Incision when Using Conditioned Media of Bone Marrow Stromal Cells Cultivated under Hypoxic Condition.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI: 10.1007/s10517-025-06363-5

N B Tikhonova, A A Temnov, V V Aleksankina, A P Aleksankin, T V Fokina, A N Sklifas, M G Aksenova, A V Elchaninov, A P Milovanov, L M Mikhaleva

引用次数: 0

Inhibition of Src Attenuates Stemness and Reverses Cisplatin Resistance of Non-Small-Cell Lung Cancer Cells.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI: 10.1007/s10517-025-06356-4

A Li, R Wang

{"title":"Inhibition of Src Attenuates Stemness and Reverses Cisplatin Resistance of Non-Small-Cell Lung Cancer Cells.","authors":"A Li, R Wang","doi":"10.1007/s10517-025-06356-4","DOIUrl":"10.1007/s10517-025-06356-4","url":null,"abstract":"The first-line chemical drug cisplatin used to treat the non-small cell lung cancer (NSCLC) often becomes ineffective due to acquired drug resistance of cancer cells. This effect and related cancer progression are related to the presence of cancer stem cells in the tumor. Tyrosine kinase Src is responsible for the appearance and development of cancer stem cells. Here, we examined the effects of Src on the oncogenic properties of cisplatin-resistant NSCLC cell lines (H358R and A549R) and the effect of inhibition of this tyrosine kinase on the sensitivity of cancer cells to cisplatin by Western blotting and immunofluorescence staining as well as migration, invasion, sphere colony, and clone formation assays. In H358R and A549R cells, the levels of phosphorylated kinase Src (pSrc) and stemness marker CD133 were elevated in comparison with the parental ones. The cisplatin-resistant cells demonstrated increased self-renewal ability and formed significantly bigger tumor spheres than their parental cells. Inhibition of Src with its inhibitor CGP77675 attenuated stemness of H358R and A549R cells; moreover, it enhanced the inhibitory effects of cisplatin on cell proliferation, migration, and invasion. The data indicated that Src-induced stemness plays an important role in developed resistance of NSCLC cells to cisplatin. Inhibition of Src attenuated stemness and acquired resistance to cisplatin, which can be beneficial for treating the cisplatin-resistant NSCLC.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"460-466"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transtraumatic Epidural Electrostimulation Promotes the Preservation of the Spinal Cord and Skeletal Muscles in Pigs.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI: 10.1007/s10517-025-06364-4

R V Shevchenko, R R Garifulin, V V Valiullin, F O Fadeev, A A Izmailov, A M Agaev, R R Islamov

{"title":"Transtraumatic Epidural Electrostimulation Promotes the Preservation of the Spinal Cord and Skeletal Muscles in Pigs.","authors":"R V Shevchenko, R R Garifulin, V V Valiullin, F O Fadeev, A A Izmailov, A M Agaev, R R Islamov","doi":"10.1007/s10517-025-06364-4","DOIUrl":"10.1007/s10517-025-06364-4","url":null,"abstract":"Morphological confirmation of the recovery of the spinal cord (SC) and the skeletal muscle (m. soleus) in both hind limbs was achieved in pigs with contusion injury in the lower thoracic region (Th8-Th9), following transtraumatic epidural electrical stimulation (TEES). Sixty days after the neurotrauma model, the anterior and posterior horns of the rostral and caudal spinal cord segments were examined using histological and immunofluorescent techniques relative to the injury epicenter. In animals with a 6-week TEES regimen at the Th5 and L2 levels, a larger area of intact gray matter, a smaller number of caspase-3+ cells undergoing apoptosis, decreased expression of the heat shock protein 27 (HSP27), inhibition of astrogliosis development, and an increase in the number of oligodendroglial cells were observed. This agrees with data on the suppression of m. soleus atrophy and the maintenance of its original phenotype. The information we previously received about the functional recovery of the spinal cord and the results of this study allow us to make conclusion about the morphofunctional post-traumatic recovery of the spinal cord under TEES conditions.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"503-506"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Characteristics of the Liver and Immunohistochemical Analysis of HSP70 Expression in Hepatocytes under the Impact of Doxorubicin and Rosuvastatin. 多柔比星和瑞舒伐他汀作用下肝脏的形态学特征及肝细胞中 HSP70 表达的免疫组化分析

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI: 10.1007/s10517-025-06365-3

E L Lushnikova, E V Koldysheva, V I Kapustina, M G Klinnikova

{"title":"Morphological Characteristics of the Liver and Immunohistochemical Analysis of HSP70 Expression in Hepatocytes under the Impact of Doxorubicin and Rosuvastatin.","authors":"E L Lushnikova, E V Koldysheva, V I Kapustina, M G Klinnikova","doi":"10.1007/s10517-025-06365-3","DOIUrl":"10.1007/s10517-025-06365-3","url":null,"abstract":"A morphological and immunohistochemical analyses of the liver of male WAG rats (n=47) were performed after isolated and combined administration of doxorubicin and rosuvastatin. Doxorubicin (7 mg/kg) was administered intraperitoneally once, while rosuvastatin (10 mg/kg) was given intragastrically daily; the animals were euthanized after 4, 14, and 21 days of experiment. The main morphological changes observed in the liver after both isolated doxorubicin administration and in combination with rosuvastatin included dystrophic and necrobiotic changes in hepatocytes, uneven congestion with the formation of thrombotic masses in the portal vein system, and pronounced perivenous edema. Isolated administration of rosuvastatin led to severe dystrophic changes in hepatocytes and fewer circulatory disturbances in the form of vascular congestion. Immunohistochemical analysis revealed nuclear, cytoplasmic, and mixed (nuclear-cytoplasmic) localization of HSP70 in hepatocytes in all experimental groups. In the liver of control rats, mixed localization of HSP70 significantly prevailed (>62% of hepatocytes; p<0.001); only nuclear localization was found in 9% of cells. When doxorubicin and rosuvastatin were administered alone or in a combination, significant translocation of HSP70 from the cytoplasm to the nucleus occurred (the index of hepatocytes with HSP70 localization in the nuclei increase by 3-4.5 times), which may reflect the cytotoxic nature of these drugs and the activation of cytoprotective mechanisms.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"507-513"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0