Hematological Parameters in Colitis-Associated Colorectal Cancer in Animals with Different Resistance to Hypoxia.

Abstract

Hematological parameters were evaluated, proinflammatory immune response indices lymphocyte-to-monocyte ratio (LMR) and neutrophil-to-lymphocyte ratio (NLR) were calculated, and iron was detected in tumor tissues by Perls' staining of histological sections in high (HR) and low resistant (LR) to hypoxia male C57BL/6 mice with colitis-associated colorectal cancer (CAC) induced by intraperitoneal injection of azoxymethane and three cycles of consumption of dextran sulfate sodium. The animals were euthanized on day 141 after azoxymethane administration. The relative number of monocytes in the blood increased in HR mice of the experimental group in comparison with the control group, and was higher than in LR animals. In LR mice with CAC, the relative number of lymphocytes and monocytes, absolute number of monocytes in the blood decreased and the NLR increased in comparison with the control; the LMR was higher than in HR animals. Regardless of hypoxia resistance, the mice developed normocytic hypochromic anemia; iron accumulation in the tumor was observed only in LR animals. The obtained data on more pronounced changes in hematological parameters in LR animals should be taken into account in the development of new approaches to the inflammatory and tumor diseases therapy, including CAC.