G A Frelikh, E A Yanovskaya, A P Lakeev, G A Chernysheva, V I Smolyakova
{"title":"改良的大鼠胆管肠双管药代动力学研究方法。","authors":"G A Frelikh, E A Yanovskaya, A P Lakeev, G A Chernysheva, V I Smolyakova","doi":"10.1007/s10517-025-06494-9","DOIUrl":null,"url":null,"abstract":"<p><p>An improved method of cannulation of the bile duct and duodenum in rats has been developed to study the kinetics of medicinal compounds in the bile. The proposed double cannulation method ensures reliable fixation and accurate placement of the catheters, prevents their twisting and loss, and allows for a comprehensive study of the processes of substance absorption and elimination. The bile was collected from animals without anesthesia, the circulation of bile acids between the liver and intestine was preserved. This method has been tested in a study of the kinetics of 2,6-diisobornyl-4-methylphenol in rat bile over 24 h after oral administration (25 mg/kg in 0.5 ml of linseed oil). The pharmacokinetic profile of the substance has been shown to be a plateau with an indistinct maximum (216.87 ± 74.39 ng/ml, after 8 h), and its slow excretion with bile is observed.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"383-387"},"PeriodicalIF":0.6000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Modified Method for Double Cannulation of Rat Bile Duct and Intestine for Pharmacokinetic Studies.\",\"authors\":\"G A Frelikh, E A Yanovskaya, A P Lakeev, G A Chernysheva, V I Smolyakova\",\"doi\":\"10.1007/s10517-025-06494-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An improved method of cannulation of the bile duct and duodenum in rats has been developed to study the kinetics of medicinal compounds in the bile. The proposed double cannulation method ensures reliable fixation and accurate placement of the catheters, prevents their twisting and loss, and allows for a comprehensive study of the processes of substance absorption and elimination. The bile was collected from animals without anesthesia, the circulation of bile acids between the liver and intestine was preserved. This method has been tested in a study of the kinetics of 2,6-diisobornyl-4-methylphenol in rat bile over 24 h after oral administration (25 mg/kg in 0.5 ml of linseed oil). The pharmacokinetic profile of the substance has been shown to be a plateau with an indistinct maximum (216.87 ± 74.39 ng/ml, after 8 h), and its slow excretion with bile is observed.</p>\",\"PeriodicalId\":9331,\"journal\":{\"name\":\"Bulletin of Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"383-387\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10517-025-06494-9\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06494-9","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/24 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Modified Method for Double Cannulation of Rat Bile Duct and Intestine for Pharmacokinetic Studies.
An improved method of cannulation of the bile duct and duodenum in rats has been developed to study the kinetics of medicinal compounds in the bile. The proposed double cannulation method ensures reliable fixation and accurate placement of the catheters, prevents their twisting and loss, and allows for a comprehensive study of the processes of substance absorption and elimination. The bile was collected from animals without anesthesia, the circulation of bile acids between the liver and intestine was preserved. This method has been tested in a study of the kinetics of 2,6-diisobornyl-4-methylphenol in rat bile over 24 h after oral administration (25 mg/kg in 0.5 ml of linseed oil). The pharmacokinetic profile of the substance has been shown to be a plateau with an indistinct maximum (216.87 ± 74.39 ng/ml, after 8 h), and its slow excretion with bile is observed.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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