磷脂酸和温度作用下细胞色素C活性中心结构的变化。

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-07-01 Epub Date: 2025-09-24 DOI:10.1007/s10517-025-06478-9

I V Kirilina, G O Stepanov, Yu A Vladimirov, A N Osipov

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引用次数: 0

摘要

细胞色素C在与线粒体磷脂形成复合物后释放到细胞质中，这是线粒体凋亡途径的基础。研究了细胞色素C复合物活性中心结构随磷脂酸浓度和温度（25、37、45℃）的变化。通过695 nm光吸收评价活性中心结构的变化，并与与细胞色素c -磷脂酸络合物过氧化物酶活性成正比的发光强度进行比较。结果发现，温度从25℃升高到45℃，细胞色素C活性中心的构象发生了类似的变化，其过氧化物酶活性也有所增加。

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Changes in the Structure of Cytochrome C Active Center under the Action of Phosphatidic Acid and Temperature.

The release of cytochrome C into the cytoplasm after formation of a complex with mitochondrial phospholipids underlays the mitochondrial pathway of apoptosis. Changes in the structure of cytochrome C complex active center depending on phosphatidic acid concentration and at different temperatures (25, 37, and 45°C) were studied. Changes in the active center structure were assessed by optical absorption at 695 nm and compared with the intensity of luminol-dependent chemiluminescence which is proportional to the peroxidase activity of cytochrome C-phosphatidic acid complex. It was found that increasing the temperature from 25 to 45°C leads to comparable changes in the conformation of cytochrome C active center and an increase in its peroxidase activity.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.