联合注射编码B细胞和t细胞免疫原的COVID-19 DNA疫苗的免疫原性

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI:10.1007/s10517-025-06461-4

M B Borgoyakova, A P Rudometov, E V Starostina, A M Zadorozhny, E V Tigeeva, V A Yakovlev, D N Kisakov, L A Kisakova, L I Karpenko, A A Ilyichev

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引用次数: 0

摘要

通过额外引入编码SARS-CoV-2蛋白（pVAXrbd）分泌受体结合域的质粒，评估了编码含有大量来自SARS-CoV-2不同蛋白的保守表位的人工多肽类t细胞免疫原的DNA疫苗增强免疫应答的可能性。在pBSI-COV-Ub和pVAXrbd联合注射的动物中，观察到特异性的体液反应和广泛特异性的细胞反应。这表明了这种方法作为一种疫苗战略的潜力。

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Immunogenicity of DNA Vaccines against COVID-19 That Encode B- and T-Cell Immunogens after Combined Injection.

The possibility of enhancing the immune response to a DNA vaccine encoding an artificial polyepitope T-cell immunogen containing a large number of conserved epitopes from different proteins of SARS-CoV-2 virus (pBSI-COV-Ub) was assessed by additional introduction of a plasmid encoding the secreted receptor-binding domain of the of SARS-CoV-2 S protein (pVAXrbd). Both a specific humoral response and a cellular response of broad specificity were observed in animals co-injected with pBSI-COV-Ub and pVAXrbd. This indicates the potential of this approach as a vaccine strategy.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.