Changes of α-Synuclein Localization in a Neuronal Precursor Graft in the Rat Model of 6-OHDA-Induced Parkinsonism.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI:10.1007/s10517-025-06469-w

D N Voronkov, A V Stavrovskaya, A S Olshanskiy, O S Lebedeva, S N Illarioshkin

{"title":"Changes of α-Synuclein Localization in a Neuronal Precursor Graft in the Rat Model of 6-OHDA-Induced Parkinsonism.","authors":"D N Voronkov, A V Stavrovskaya, A S Olshanskiy, O S Lebedeva, S N Illarioshkin","doi":"10.1007/s10517-025-06469-w","DOIUrl":null,"url":null,"abstract":"<p><p>In the experiment with transplantation of neuronal mesencephalic precursors derived from induced pluripotent stem cells into the striatum of rats with unilateral 6-OHDA destruction of the substantia nigra, we evaluated changes in the immunostaining for α-synuclein during differentiation and maturation of grafted neurons 1, 3, and 6 months after the surgery. The content of α-synuclein in transplanted neurons peaked by the third month after surgery, as well as changes in the nuclear-cytoplasmic ratio of α-synuclein in neurons by the 3rd and 6th months relative to the 1st month after transplantation were revealed. Changes in α-synuclein localization and content were associated with a gradual decrease in doublecortin expression and an increase in immunostaining for neuronal enolase and synaptophysin as the graft matured. The high content of total α-synuclein in developing neurons may be a key factor of spreading of α-synuclein toxic species in graft cells and a cause of cell damage.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"254-259"},"PeriodicalIF":0.6000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06469-w","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

In the experiment with transplantation of neuronal mesencephalic precursors derived from induced pluripotent stem cells into the striatum of rats with unilateral 6-OHDA destruction of the substantia nigra, we evaluated changes in the immunostaining for α-synuclein during differentiation and maturation of grafted neurons 1, 3, and 6 months after the surgery. The content of α-synuclein in transplanted neurons peaked by the third month after surgery, as well as changes in the nuclear-cytoplasmic ratio of α-synuclein in neurons by the 3rd and 6th months relative to the 1st month after transplantation were revealed. Changes in α-synuclein localization and content were associated with a gradual decrease in doublecortin expression and an increase in immunostaining for neuronal enolase and synaptophysin as the graft matured. The high content of total α-synuclein in developing neurons may be a key factor of spreading of α-synuclein toxic species in graft cells and a cause of cell damage.

查看原文本刊更多论文

α-突触核蛋白在6-羟多巴胺诱导的帕金森大鼠神经前体移植物中的定位变化。

在将诱导多能干细胞衍生的神经元中脑前体移植到单侧6- ohda破坏黑质大鼠纹状体的实验中，我们观察了移植神经元在术后1、3和6个月分化和成熟过程中α-突触核蛋白的免疫染色变化。观察移植神经元α-突触核蛋白含量在术后第3个月达到峰值，并观察移植后第3、6个月神经元α-突触核蛋白核质比在移植后第1个月的变化。随着移植物成熟，α-突触核蛋白的定位和含量的变化与双皮质素表达的逐渐减少以及神经元烯醇化酶和突触素免疫染色的增加有关。发育中的神经元中总α-突触核蛋白的高含量可能是α-突触核蛋白毒性物质在移植物细胞中扩散的关键因素和细胞损伤的原因之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.