M N Yurova, E I Fedoros, A B Danilova, T L Nekhaeva, N V Emelyanova, A V Grigorevskaya, I G Popovich, I A Baldueva
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Ex Vivo Irradiation Protocol in Murine Lymph Node Cells.
The aim was to test the protocol for obtaining lymphoid tissue from mouse lymph nodes and characterize the level of DNA damage with analysis of the repair and apoptosis protein markers in ex vivo model of cell irradiation. The lymph node cell suspension was exposed to X-ray irradiation at a dose of 2 Gy and compared with a sham-irradiated (0 Gy) control. Ex vivo irradiation at a dose of 2 Gy after 3 h caused a 7-fold increase in DNA damage level determined in DNA comet assay. A significant increase in the level of repair proteins γH2AX (by 3.7 times), Rad51 (by 2.2 times), and XPA (by 28 times), cell cycle arrest proteins Cdc25A (by 2.6 times), p21 (by 10 times), and p16 (by 5 times), and pro-apoptotic protein Bax (by 3.8 times) was noted, without significant changes in Bcl-2 level. The proposed technique allows quick obtaining lymphoid tissue samples suitable for analysis of the radiosensitivity of lymphoid cells in mice of various strains, changes in their sensitivity after or in combination with the primary damaging exposure.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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