Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online Solid-Phase Extraction.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-29 DOI:10.1007/s10517-025-06474-z

D V Bychenkov, V A Ioutsi, I V Kirilina, M V Ovcharov, M A Antsupova, A K Lubenskaya, L K Abdullaev, S A Pantsernaya, N G Mokrysheva

引用次数: 0

Abstract

We propose a method for the quantitative determination of serotonin in blood serum using high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and online solid-phase extraction in a chromatographic system. This approach allows serotonin quantification in blood serum within a range of 0.5-2000 ng/ml, partially automates the sample preparation process, and minimizes the influence of matrix components. The method is designed for the quantitative measurement of serotonin levels in patients with various deviations of serotonin levels and associated diseases.

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高效液相色谱-串联质谱在线固相萃取法分析血清血清素。

我们提出了一种高效液相色谱串联质谱检测（HPLC-MS/MS）和在线固相萃取在色谱系统中定量测定血清中血清素的方法。该方法允许在0.5-2000 ng/ml范围内定量血清中的血清素，部分自动化样品制备过程，并最大限度地减少基质成分的影响。本方法适用于血清素水平偏差及相关疾病患者血清素水平的定量测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.