The Influence of Bisphenol A on the Molecular Biological Characteristics of Human Colon Adenocarcinoma HT-29 Culture Cells.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI:10.1007/s10517-025-06447-2

E A Miroshnichenko, M A Diatroptova, A I Alekseeva, A D Gerasimov, A M Kosyreva

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Abstract

Researchers attribute increased incidence of colorectal cancer to environmental pollution with endocrine disruptors, including the estrogen-like substance bisphenol A (BPA). The proliferative activity, viability, and mRNA expression levels of the PIK3CA, NFkBp65, VEGFA, ESR1, HIF1a, PTEN, TP53, CD44, and CDH1 genes were evaluated in human colon adenocarcinoma HT-29 cell culture incubated with different concentrations of BPA (5, 25, and 50 μM) for 24 and 96 h. Short-term (24 h) incubation with BPA did not lead to pronounced biological effects. During prolonged incubation (96 h), 5 μM BPA decreased VEGFA mRNA expression and increased HIF1a mRNA expression, 25 μM BPA increased the expression of PIK3CA, ESR1, and HIF1a mRNAs and decreased VEGFA and CD44, and 50 μM BPA increased the expression of ESR1 mRNA and was cytotoxic. Hence, the duration of incubation and the concentration of BPA in the culture medium affect the progression of the colon cancer and its malignancy.

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双酚A对人结肠腺癌HT-29培养细胞分子生物学特性的影响

研究人员将结直肠癌发病率的增加归因于环境污染中的内分泌干扰物，包括雌激素样物质双酚A （BPA）。研究了PIK3CA、NFkBp65、VEGFA、ESR1、HIF1a、PTEN、TP53、CD44和CDH1基因在不同浓度BPA （5 μM、25 μM和50 μM）培养24和96 h后的增殖活性、活力和mRNA表达水平。BPA短期（24 h）培养未产生明显的生物学效应。延长孵育时间（96 h）后，5 μM BPA降低了VEGFA mRNA的表达，增加了HIF1a mRNA的表达；25 μM BPA增加了PIK3CA、ESR1和HIF1a mRNA的表达，降低了VEGFA和CD44的表达；50 μM BPA增加了ESR1 mRNA的表达，并具有细胞毒性。因此，培养时间和培养基中BPA浓度影响结肠癌及其恶性肿瘤的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.