E V Tigeeva, E V Shaburova, D V Antonets, D N Kisakov, M B Borgoyakova, E V Starostina, V A Yakovlev, L A Kisakova, N B Rudometova, A P Rudometov, L I Karpenko
{"title":"The Immunogenicity of an Artificial Tick-Borne Encephalitis Virus Polyepitope Immunogen May Depend on Spacers Flanking Epitope.","authors":"E V Tigeeva, E V Shaburova, D V Antonets, D N Kisakov, M B Borgoyakova, E V Starostina, V A Yakovlev, L A Kisakova, N B Rudometova, A P Rudometov, L I Karpenko","doi":"10.1007/s10517-025-06462-3","DOIUrl":null,"url":null,"abstract":"<p><p>The development of artificial polyepitope immunogens based on the conserved CD4<sup>+</sup> and CD8<sup>+</sup> epitopes from different virus proteins combined into a single molecule is a promising approach in the development of vaccines for activating a specific T-cell response. Selection of spacer sequences flanking the epitopes within the construct and providing the optimal processing of such immunogens within the cell is an important step in the design of such vaccines. We report the results of designing polyepitope T-cell immunogens of tick-borne encephalitis virus that differ in the presence or absence of optimally selected alanine spacers between epitopes, as well as the data of comparative analysis of immunogenic properties of DNA vaccines encoding the obtained polyepitope immunogens.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"223-227"},"PeriodicalIF":0.6000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06462-3","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
The development of artificial polyepitope immunogens based on the conserved CD4+ and CD8+ epitopes from different virus proteins combined into a single molecule is a promising approach in the development of vaccines for activating a specific T-cell response. Selection of spacer sequences flanking the epitopes within the construct and providing the optimal processing of such immunogens within the cell is an important step in the design of such vaccines. We report the results of designing polyepitope T-cell immunogens of tick-borne encephalitis virus that differ in the presence or absence of optimally selected alanine spacers between epitopes, as well as the data of comparative analysis of immunogenic properties of DNA vaccines encoding the obtained polyepitope immunogens.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
