Bulletin of Experimental Biology and Medicine最新文献_第6页

Secretome and Cellular Composition of Peripheral Blood Mononuclear Cells Cultured in the Presence of Cell Feeder or Interleukins. 白细胞介素和细胞饲养剂对外周血单核细胞分泌组和细胞组成的影响。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06449-0

J M Rozenberg, R V Zhurikov, A S Oleinik, S A Roumiantsev, E V Abakushina

{"title":"Secretome and Cellular Composition of Peripheral Blood Mononuclear Cells Cultured in the Presence of Cell Feeder or Interleukins.","authors":"J M Rozenberg, R V Zhurikov, A S Oleinik, S A Roumiantsev, E V Abakushina","doi":"10.1007/s10517-025-06449-0","DOIUrl":"10.1007/s10517-025-06449-0","url":null,"abstract":"Peripheral blood mononuclear cells-derived immune cells are widely used in clinical practice, in particular for cancer therapy. The growth conditions are critical for the composition and functional activity of cells. Mononuclear cells were cultured for 15 days under different conditions: in the presence of K562 cell feeder expressing 4-1BBL and mbIL-21I, or without feeder, but in the presence of L-21 or with different combinations of cytokines (IL-2, IL-15, IL-21, and IL-12) without the feeder and the cytokine profile (48 cytokines) of the culture medium in the middle (day 7) and at the end (day 14) of culturing was compared. In the presence of the feeder, the cells produced IFNγ, RANTES, MIP-1α, IL-9, IL-6, and CF-CSF in high concentrations (> 5000 pg/ml) and less intensively produced MDC; NK cells predominated in the culture. These cytokines promote proliferation of immune cells, participate in antitumor response (IFNγ and RANTES) and have a dual effect on the growth of some tumor cell lines (IL-6, IL-9, and VEGF). The conditioned medium, especially the medium from mononuclears grown without feeder, inhibited the growth of lung cancer cell line A549. The studied methods for mononuclear cell culturing increase the functional and antitumor activities of NK cells via stimulation of cytokine production.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"147-153"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infarct-Limiting Efficacy of K_ATP Channel Activators and Nitric Oxide Donors under Conditions of Their Chronic Administration and Diet-Induced Metabolic Syndrome Conditions in Rats. 大鼠慢性给药和饮食诱导代谢综合征条件下KATP通道激活剂和一氧化氮供体的梗死限制作用

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06421-y

N V Naryzhnaya, A V Mukhomedzyanov, M Kilin, N S Voronkov, B K Kurbatov, L N Maslov

引用次数: 0

Thymus of Female Wistar Rats after Photodynamic Therapy of Experimental Breast Cancer in Comparison with Combination of Photodynamic Therapy and Surgical Treatment of Breast Cancer. 实验性乳腺癌光动力治疗后雌性Wistar大鼠胸腺与光动力治疗联合手术治疗乳腺癌的比较

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06433-8

O V Kazakov, A V Kabakov, A F Poveshchenko, V N Cherkas, N R Bodrova

{"title":"Thymus of Female Wistar Rats after Photodynamic Therapy of Experimental Breast Cancer in Comparison with Combination of Photodynamic Therapy and Surgical Treatment of Breast Cancer.","authors":"O V Kazakov, A V Kabakov, A F Poveshchenko, V N Cherkas, N R Bodrova","doi":"10.1007/s10517-025-06433-8","DOIUrl":"10.1007/s10517-025-06433-8","url":null,"abstract":"A histological study of structural changes in the thymus of female Wistar rats with chemically induced breast cancer (BC) after photodynamic therapy (PDT) and a combination of PDT and surgical treatment was conducted. After PDT, the thymus showed restoration of the cortical and medullary substance areas, as well as glandular tissue, to the values of the intact control in comparison with the pathological control (BC without treatment). PDT influences both positive and negative selection processes and the activity of T-lymphocyte differentiation processes (the number of epithelial cells and macrophages increases, the number of cells with pyknotic nuclei in the cortical and medullary substance decreases). In the cortico-medullary zone of the thymus, the number of small and medium lymphocytes increases, which may indicate an increase in lymphocyte migration from the thymus. After PDT and subsequent resection of a BC, structural changes in the thymus may indicate a decrease in its lymphopoietic function, activity of both positive and negative T-cell selection processes, as well as a decrease in the activity of T-lymphocyte differentiation processes and their migration from the thymus.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"81-85"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Lysyl Oxidase Expression and Vascular Bed in Human Uveal Melanoma. 人葡萄膜黑色素瘤赖氨酸氧化酶表达及血管床分析。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06441-8

A Yu Shatruk, N P Bgatova, N V Kononova, A V Eremina, A N Trunov, V V Chernykh, Iu S Taskaeva

引用次数: 0

Cell Therapy for Type 1 Diabetes Mellitus: a Review of Clinical Trials. 1型糖尿病的细胞治疗：临床试验综述

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06444-5

M I Danilevskii, O V Zakharova, N N Skaletskiy, Yu B Basok, S A Roumiantsev, A V Lyundup

引用次数: 0

Expression of Wnt/β-Catenin Pathway Markers in Ascites Tumor Cells in Ovarian Cancer. Wnt/β-Catenin通路标志物在卵巢癌腹水肿瘤细胞中的表达

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06438-3

T V Abakumova, D R Dolgova, I I Antoneeva, T P Gening, I R Myagdieva, Yu S Slesareva

引用次数: 0

Comparative Analysis of Quantitative Indicators of Progesterone Receptor Protein and mRNA Expression in Tumor Cell Lines. 肿瘤细胞系孕酮受体蛋白及mRNA表达定量指标的比较分析。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06448-1

I P Romanov, T A Bogush, A A Alimov, A Y Kuzevanova, A M Scherbakov, V S Kosorukov

引用次数: 0

Study of the Bactericidal Efficacy of Short-Wave UV Irradiation on Pathogenic Klebsiella pneumoniae, Moraxella catarrhalis, and Kocuria rhizophila by Confocal Laser Scanning Microscopy. 短波紫外线照射对致病性肺炎克雷伯菌、卡他莫拉菌和嗜根结核杆菌杀菌效果的共聚焦激光扫描显微镜研究。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06439-2

O Yu Manzenyuk, O V Kalmantaeva, M A Kotov, M A Makarova, V V Firstova, G M Titareva, A N Shemyakin, M Yu Yakimov, N G Solovyov

{"title":"Study of the Bactericidal Efficacy of Short-Wave UV Irradiation on Pathogenic Klebsiella pneumoniae, Moraxella catarrhalis, and Kocuria rhizophila by Confocal Laser Scanning Microscopy.","authors":"O Yu Manzenyuk, O V Kalmantaeva, M A Kotov, M A Makarova, V V Firstova, G M Titareva, A N Shemyakin, M Yu Yakimov, N G Solovyov","doi":"10.1007/s10517-025-06439-2","DOIUrl":"10.1007/s10517-025-06439-2","url":null,"abstract":"The effect of UV radiation in the range of 185-254 nm on the viability of stress-resistant dormant forms of Moraxella catarrhalis, Kocuria rhizophila and biofilm-forming Klebsiella pneumoniae was studied. This bacterial form is much more resistant to UV irradiation in comparison with vegetative (planktonic) cultures. The antimicrobial efficacy of the experimental UV emitter (Ishlinsky Institute for Problems in Mechanics, Russian Academy of Sciences) was studied by confocal microscopy with differentiated staining of live/dead cells of K pneumoniae, M. catarrhalis, and K. rhizophila. For the first time, synergistic effects of UV irradiation in combination with biological and physical factors were demonstrated. When phage-encoded depolymerase Dep_kpv74 was added and followed by UV irradiation, the number of dead cells in K. pneumoniae biofilms increased from 81 to 91%. The combined effect of UV radiation and ozone on K. rhizophila dormant form resulted in cell death in 94% of cases. M. catarrhalis dormant forms were more resistant to UV irradiation (55% dead cells) in comparison with irradiated vegetative cells (72% dead cells). The results obtained have great practical and fundamental significance. They demonstrate the high survival rates of stress-resistant forms of pathogenic bacteria in the environment. They also show that new bactericidal devices operated at different wavelengths are required to prevent the spread of pathogens causing infectious diseases.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"45-49"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Intermittent Hypoxia on the Expression of Glucocorticoid Receptors and Hypoxia-Inducible Factor in Rat Brain. 间歇性缺氧对大鼠脑糖皮质激素受体及缺氧诱导因子表达的影响。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI: 10.1007/s10517-025-06436-5

E A Rybnikova, K A Baranova, M Y Zenko

引用次数: 0

Morphological Features of the Myocardium in db/db Mice with Genetically Determined Leptin Resistance and Glucose Metabolism Disorders after Systematic Administration of Recombinant Apolipoprotein A-I. 系统给药重组载脂蛋白A-I后遗传决定瘦素抵抗和糖代谢紊乱的db/db小鼠心肌形态学特征

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-19 DOI: 10.1007/s10517-025-06451-6

E L Lushnikova, R A Knjazev, E I Yuzhik, M V Kotova, N V Trifonova, N A Palchikova, M G Klinnikova, O I Kuzminova, M I Voevoda

{"title":"Morphological Features of the Myocardium in db/db Mice with Genetically Determined Leptin Resistance and Glucose Metabolism Disorders after Systematic Administration of Recombinant Apolipoprotein A-I.","authors":"E L Lushnikova, R A Knjazev, E I Yuzhik, M V Kotova, N V Trifonova, N A Palchikova, M G Klinnikova, O I Kuzminova, M I Voevoda","doi":"10.1007/s10517-025-06451-6","DOIUrl":"10.1007/s10517-025-06451-6","url":null,"abstract":"We studied structural reorganization of the myocardium and the indices of glucose metabolism in db/db mice of different ages and the effect of administration of recombinant apolipoprotein A-I (rApoA-I) (subcutaneously at a dose of 7 mg/kg body weight once a week) on these parameters. A 2.9-fold (p < 0.001) increase in the plasma concentration of glucose and a 6.5-fold (p < 0.001) increase in the concentration of insulin were shown in db/db mice at the age of 24 weeks in comparison with the age-matched control (C57Bl mice), which reflected glucose metabolism disorders. Administration of rApoA-I starting from the age of 8 weeks led to a 10.2% decrease in the plasma glucose concentration in mice at the age of 24 weeks, which, however, remained elevated by 2-2.6 times (p < 0.001) in comparison with the control. Administration of rApoA-I did not affect insulin levels. The main structural feature of the myocardium in db/db mice were lytic damage to cardiomyocytes with perinuclear \"depletion\" phenomena progressing with age (the proportion of these cardiomyocytes increased from 50% at the age of 10 weeks to 66% at the age of 24 weeks) and a decrease in the volume density of capillaries relative to the control (by 26-44%, p < 0.05) and also progressing with age. In db/db mice weekly treated with rApoA-I, degenerative and necrobiotic changes in endothelial cells and smooth muscle cells in intramural arteries were less pronounced or absent. The proportion of cardiomyocytes with lytic changes decreased in each age group after treatment with rApoA-I (by 11-22%, p < 0.05), but the pattern of myocardial remodeling did not change significantly, i.e., the volume ratios of capillaries to cardiomyocytes and connective tissue to cardiomyocytes remained reduced.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"154-161"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0