Morphological and Molecular Peculiarities of Healing of Uterine Incision when Using Conditioned Media of Bone Marrow Stromal Cells Cultivated under Hypoxic Condition.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI:10.1007/s10517-025-06363-5

N B Tikhonova, A A Temnov, V V Aleksankina, A P Aleksankin, T V Fokina, A N Sklifas, M G Aksenova, A V Elchaninov, A P Milovanov, L M Mikhaleva

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Abstract

We studied the effect of conditioned media (CM) from cultivated bone marrow stromal cells grown at 10% O₂ on extracellular matrix and vascular component in the healing zone after surgical incision of the uterine wall in Sprague-Dawley rats (n=17). Control group (n=10) received no treatment. On days 5 and 15 after the surgery, the expression of CoL1a1, CoL4a, MMP9, TIMP1, and FGF2 genes was evaluated and a morphological study was carried out. On day 5, CoL1a1 expression, CD34⁺ cell content, and the area of newly formed tissue were lower in the experimental group. On day 15, the expression of MMP9, TIMP1, FGF2, and CoL1a1 genes in the control group was lower, while CD34⁺ cell content and area of healing zone were higher. Thus, the application of CM reduced the damage area and accelerated the recovery process after surgical full-thickness incision of the uterine wall.

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缺氧条件下培养骨髓基质细胞对子宫切口愈合的形态学和分子特性。

我们研究了10% O2培养的骨髓基质细胞条件培养基（CM）对Sprague-Dawley大鼠（n=17）子宫壁手术切口后愈合区细胞外基质和血管成分的影响。对照组（n=10）不进行治疗。术后第5、15天检测CoL1a1、CoL4a、MMP9、TIMP1、FGF2基因的表达并进行形态学研究。第5天，实验组CoL1a1表达量、CD34+细胞含量、新生组织面积均降低。第15天，对照组MMP9、TIMP1、FGF2、CoL1a1基因表达较低，而CD34+细胞含量和愈合区面积较高。因此，CM的应用减少了子宫壁全层切开后的损伤面积，加快了子宫壁的恢复过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.