Morphological and Molecular Peculiarities of Healing of Uterine Incision when Using Conditioned Media of Bone Marrow Stromal Cells Cultivated under Hypoxic Condition.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI:10.1007/s10517-025-06363-5

N B Tikhonova, A A Temnov, V V Aleksankina, A P Aleksankin, T V Fokina, A N Sklifas, M G Aksenova, A V Elchaninov, A P Milovanov, L M Mikhaleva

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引用次数: 0

Abstract

We studied the effect of conditioned media (CM) from cultivated bone marrow stromal cells grown at 10% O₂ on extracellular matrix and vascular component in the healing zone after surgical incision of the uterine wall in Sprague-Dawley rats (n=17). Control group (n=10) received no treatment. On days 5 and 15 after the surgery, the expression of CoL1a1, CoL4a, MMP9, TIMP1, and FGF2 genes was evaluated and a morphological study was carried out. On day 5, CoL1a1 expression, CD34⁺ cell content, and the area of newly formed tissue were lower in the experimental group. On day 15, the expression of MMP9, TIMP1, FGF2, and CoL1a1 genes in the control group was lower, while CD34⁺ cell content and area of healing zone were higher. Thus, the application of CM reduced the damage area and accelerated the recovery process after surgical full-thickness incision of the uterine wall.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.