Bulletin of Experimental Biology and Medicine最新文献_第7页

A New Method of Intraoperative Modeling of Liver Cirrhosis in an Experiment. 一种新的肝硬化术中模拟实验方法。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-06 DOI: 10.1007/s10517-025-06319-9

A Yu Laptieva, A A Andreev, V V Shishkina, A A Glukhov, A P Ostroushko, M V Aralova, S N Boev, P A Konovalov, A V Puchnina

引用次数: 0

Production of the Key Immunoregulatory Cytokines and the Content of FoxP3⁺ T-Regulatory Lymphocytes in the Epicardial and Thymus Adipose Tissue in Patients with Coronary Heart Disease. 冠心病患者心外膜和胸腺脂肪组织中关键免疫调节细胞因子的产生及FoxP3+ t调节淋巴细胞的含量

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06304-2

I V Kologrivova, A A Dmitriukov, N V Naryzhnaya, E S Kravchenko, O A Kharitonova, A I Vyrostkova, A S Krapivina, S L Andreev, O A Koshelskaya, T E Suslova

{"title":"Production of the Key Immunoregulatory Cytokines and the Content of FoxP3+ T-Regulatory Lymphocytes in the Epicardial and Thymus Adipose Tissue in Patients with Coronary Heart Disease.","authors":"I V Kologrivova, A A Dmitriukov, N V Naryzhnaya, E S Kravchenko, O A Kharitonova, A I Vyrostkova, A S Krapivina, S L Andreev, O A Koshelskaya, T E Suslova","doi":"10.1007/s10517-025-06304-2","DOIUrl":"https://doi.org/10.1007/s10517-025-06304-2","url":null,"abstract":"FoxP3+ T-regulatory (Treg) lymphocytes and cytokine production by cells from the stromal vascular fraction (SVF) of epicardial (EAT) and thymus (TAT) adipose tissue of 42 patients with chronic coronary heart disease (CHD) were studied. In the SVF of TAT in patients with Gensini Score (GS)≥74 (the most severe atherosclerosis), the production of IL-1β, TNF, IL-4, and IFNγ was higher, while FoxP3 translocation into the nucleus was lower than in patients with GS<74. The GS index directly correlated with the production of IL-4, IL-1β, and TNF by cells of the SVF of TAT, and inversely - with the production of TNF, IL-17, and IL-10 by cells of the SVF of EAT. The proportion of CD25hiFoxP3+Treg with FoxP3 nuclear translocation in TAT inversely correlated with IFNγ production in TAT and directly correlated with IL-4 production in EAT. The results obtained suggest that the severity of coronary atherosclerosis is interrelated with the ability of SVF cells of EAT and TAT to produce cytokines and the properties of FoxP3+Treg lymphocytes.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"184-188"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hespintor Negative Regulation of PI3K/Akt Pathway Induces Cell Cycle Arrest of Hepatocellular Carcinoma. Hespintor负调控PI3K/Akt通路诱导肝癌细胞周期阻滞

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06314-0

J Sun, Y Z Lun, B Liu, W Dong

{"title":"Hespintor Negative Regulation of PI3K/Akt Pathway Induces Cell Cycle Arrest of Hepatocellular Carcinoma.","authors":"J Sun, Y Z Lun, B Liu, W Dong","doi":"10.1007/s10517-025-06314-0","DOIUrl":"https://doi.org/10.1007/s10517-025-06314-0","url":null,"abstract":"The mechanism of Hespintor (a protein of serpin family) inhibitory action on the growth of inoculated hepatocellular carcinoma was studied in a model of human hepatoma in nude mice by using on long-noncoding RNA (lncRNA) sequencing. Two days after tumor transplantation, Hespintor or normal saline was injected into the caudal vein at a dose of 15 μg/kg (2 times a week over 4 weeks). The tumors were isolated in 4 weeks after subcutaneous injection of human hepatoma MHCC97-H cells. In Hespintor and control groups, the complementary DNA libraries of tumor tissues were established, and transcriptome sequencing was performed. Based on RNA-sequencing data, the differentially expressing lncRNA genes (DEGs lncRNA) were obtained, and functional enrichment and interaction analyses were performed to find the regulatory gene sets. Then, the network module division method was employed to identify the key genes of the Hespintor action, as well as to build the regulatory network and critical pathways associated with the key genes with validation of the results by Western blotting. The target gene sets regulated by DEGs lncRNA were mainly enriched in cell behavior, transcriptional regulation, and cell cycle. The PI3K/Akt signaling pathway related to the revealed gene sets plays a leading role in the antitumor action of Hespintor, targeted by this serpin to down-regulate expression levels of the cell cycle regulatory proteins Cyclin D1, P-Rb, CDK4, and CDK6, thereby arresting the cell cycle in G1/S phase.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"237-243"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota of the Mammary Gland in Wistar Rats with Chemically Induced Breast Cancer after Treatment. 化学诱导乳腺癌 Wistar 大鼠治疗后乳腺的微生物区系

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06311-3

A F Poveshchenko, A V Kabakov, N R Bodrova, V I Kapustina, O V Kazakov, E V Koldysheva, V N Cherkas, V N Afonyushkin

{"title":"Microbiota of the Mammary Gland in Wistar Rats with Chemically Induced Breast Cancer after Treatment.","authors":"A F Poveshchenko, A V Kabakov, N R Bodrova, V I Kapustina, O V Kazakov, E V Koldysheva, V N Cherkas, V N Afonyushkin","doi":"10.1007/s10517-025-06311-3","DOIUrl":"https://doi.org/10.1007/s10517-025-06311-3","url":null,"abstract":"We conducted a comparative study of the mammary gland microbiota in female Wistar rats and the microbiota associated with breast cancer (BC) induced by the administration of N-methyl-N-nitrosourea, after surgical treatment, photodynamic therapy (PDT), and chemotherapy (CT). Selective nutrient media and a smear-fingerprint technique were used to study the microbiota. Staphylococcus, Streptococcus, and Lactobacillus were found in the mammary glands of intact rats. The mean number of Staphylococcus CFU increased statistically significantly in all groups of rats with induced BC, compared to intact animals: by 8 times in the BC without treatment group, by 15% in BC+surgery, by 10 times in BC+PDT, by 4 times in BC+PDT+CT, and by 13 times in BC+CT. The number of Streptococcus CFU also increased in all experimental groups: by 15.5 times in the BC without treatment group, by 4 times in BC+surgery, by 2.6 times in BC+PDT, by 4.5 times in BC+PDT+CT, and by 3.5 times in BC+CT. The number of Lactobacillus CFU significantly increased only in the BC+PDT group (by 2.5 times). The development of the microbiota in the tumor allows evaluating the impact of various types of therapy on the growth of opportunistic microorganisms. The results suggest that doxorubicin combined with PDT has the most potent effect on changes to the tumor microbiota.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"223-226"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Somatostatin Receptors in the Small Intestine during Postnatal Ontogenesis. 出生后个体发育过程中小肠生长抑素受体的表达。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06303-3

A I Emanuilov, E S Shirina, P M Masliukov

引用次数: 0

Calcium Ion Attenuates Transforming Growth Factor β1-Induced Extracellular Matrix Accumulation by Inducing Smad2 Degradation through the Proteasome Pathway. 钙离子通过蛋白酶体途径诱导Smad2降解，从而减弱转化生长因子β1诱导的细胞外基质积累

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06317-x

Jialin Li, Jiawen Zhang, Meng Zhang, Suzhen Wu

{"title":"Calcium Ion Attenuates Transforming Growth Factor β1-Induced Extracellular Matrix Accumulation by Inducing Smad2 Degradation through the Proteasome Pathway.","authors":"Jialin Li, Jiawen Zhang, Meng Zhang, Suzhen Wu","doi":"10.1007/s10517-025-06317-x","DOIUrl":"10.1007/s10517-025-06317-x","url":null,"abstract":"Extracellular Ca2+ is the first ligand that has been confirmed to function by activating the calcium-sensing receptor (CaSR), a member of G-protein coupled receptors. CaSR controls not only calcium homeostasis, but also plays a pivotal role in many cellular processes such as cell proliferation and apoptosis; moreover, it is implicated in the development of cardiovascular diseases. TGF-β/Smads signaling pathway is a classical pathway of renal fibrosis. Here we used a culture of mesangial cells to evaluate the mechanisms of the renoprotective effects of Ca2+. We found that Ca2+ inhibits TGF-β-induced phosphorylation of Smad2 and deposition of fibronectin (FN), in turn, down-regulation of FN and phosphorylation of Smad2 was closely related to the degradation of Smad2 through the proteasomal pathway. We found that Ca2+ only downregulates the expression of Smad2 at the protein level, but has no effect on its gene expression. However, Ca2+ could downregulate TGF-β-induced expression of FN both at the protein and gene level. Hence, Smad2 acts as a transcription factor of FN, and its degradation definitely inhibits the expression of its target gene FN.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"255-260"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Rhythmic Optical Stimulation on Temporal Parameters of Human Sensorimotor Response and Their Relation to the Spectral Characteristics of the Initial EEG. 有节奏的光学刺激对人类感觉运动反应时相参数的影响及其与初始脑电图频谱特征的关系

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06302-4

N A Karatygin, I I Korobeinikova, Y A Venerina, M I Maleto, S S Pertsov

{"title":"The Effect of Rhythmic Optical Stimulation on Temporal Parameters of Human Sensorimotor Response and Their Relation to the Spectral Characteristics of the Initial EEG.","authors":"N A Karatygin, I I Korobeinikova, Y A Venerina, M I Maleto, S S Pertsov","doi":"10.1007/s10517-025-06302-4","DOIUrl":"https://doi.org/10.1007/s10517-025-06302-4","url":null,"abstract":"The effect of optical stimulation at a frequency of 10 Hz (OS10Hz) on temporal parameters of sensorimotor activity in healthy subjects (n=32) was studied. The expression of the activation response was determined by the ratio of spectral power values (SPα2, μV2) of the high frequency (10-13 Hz) subrange of the α-rhythm of the initial EEG with closed and opened eyes and the frequency of the maximum α-peak (IAPF). A test for simple motor reaction time was performed under normal and OS10Hz conditions. According to the change in the variability of simple motor reaction time (SDSMRT) under OS10Hz compared to the normal conditions, the subjects were classified into two groups: those who showed a decrease (group 1) and those who showed an increase (group 2) in SDSMRT. The subjects of group 1 had significantly higher IAPF (in the O1 lead with eyes closed) and activation reaction intensity (in the O2, O1, P4, P3, and F3 leads) compared to the subjects of group 2. The obtained data suggest that OS10Hz changes the parameters of psychomotor reactions of an individual, and the direction of changes is mediated by individual features of the initial EEG.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"177-180"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Resonance Imaging of Monocrotaline-Induced Pulmonary Hypertension in Rats Using Radial Scanning with Retrospective Gating. 回顾性门控放射扫描对大鼠单罂粟碱所致肺动脉高压的磁共振成像研究。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-06 DOI: 10.1007/s10517-025-06316-y

P S Kolesnikova, O S Pavlova, M V Gulyaev, T A Kuropatkina, Yu A Pirogov

引用次数: 0

Stratification of Experimental LPS-Induced Systemic Inflammatory Response by Expression Level of Hif1a and NFkb Genes. Hif1a和NFkb基因表达水平对实验性脂多糖诱导的全身炎症反应的分层。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-06 DOI: 10.1007/s10517-025-06318-w

A M Kosyreva, D Sh Dzhalilova, E A Miroshnichenko, O V Makarova

引用次数: 0

Changes in Functional Activity of Platelets under the Influence of Submicron Particles Based on Mesoporous Silica and pH-Sensitive Polymers. 基于介孔二氧化硅和ph敏感聚合物的亚微米颗粒影响下血小板功能活性的变化。