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Effect of Human Macrophage Conditioned Media on TGF-β-Induced Differentiation of Lung Fibroblasts. 人巨噬细胞条件培养基对TGF-β诱导的肺成纤维细胞分化的影响
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI: 10.1007/s10517-025-06353-7
A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh
{"title":"Effect of Human Macrophage Conditioned Media on TGF-β-Induced Differentiation of Lung Fibroblasts.","authors":"A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh","doi":"10.1007/s10517-025-06353-7","DOIUrl":"10.1007/s10517-025-06353-7","url":null,"abstract":"<p><p>We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"442-446"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Redox-Regulated Cell Response Mechanisms to Nitrosyl Iron Complex with N-Ethylthiourea in Human Mesenchymal Stem Cells In Vitro. 体外人间充质干细胞对n -乙基硫脲配合物亚硝基铁的氧化还原调控细胞反应机制
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI: 10.1007/s10517-025-06367-1
A A Balakina, V I Amozova, T S Stupina, N A Sanina
{"title":"Redox-Regulated Cell Response Mechanisms to Nitrosyl Iron Complex with N-Ethylthiourea in Human Mesenchymal Stem Cells In Vitro.","authors":"A A Balakina, V I Amozova, T S Stupina, N A Sanina","doi":"10.1007/s10517-025-06367-1","DOIUrl":"10.1007/s10517-025-06367-1","url":null,"abstract":"<p><p>We studied the processes of cell death and intracellular accumulation of free radicals, as well as activity of redox-sensitive transcription factors Nrf2 and NF-κB under the action of nitrosyl iron complex with N-ethylthiourea (ETU) on a model of human mesenchymal stem cells in vitro. ETU complex exhibits a pronounced prooxidant and cytotoxic effects accompanied by enhanced intracellular accumulation of nitrogen monoxide, induction of apoptotic cell death, activation of Nrf2/HO-1 pathways, and suppression of proinflammatory NF-κB-mediated reactions. A significant role of redox-dependent cellular processes in the mechanisms of the cytotoxic effect of nitrosyl iron complexes is shown.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"520-528"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Features of Histogenesis of Organ-Specific Regenerate in the Experiment. 实验中器官特异性再生组织发生的特点。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI: 10.1007/s10517-025-06371-5
O S Kosareva, M N Drovosekov, S V Savchenko, T A Ageeva, N A Ivanova, S V Ostapets, E V Nikitenko
{"title":"Features of Histogenesis of Organ-Specific Regenerate in the Experiment.","authors":"O S Kosareva, M N Drovosekov, S V Savchenko, T A Ageeva, N A Ivanova, S V Ostapets, E V Nikitenko","doi":"10.1007/s10517-025-06371-5","DOIUrl":"10.1007/s10517-025-06371-5","url":null,"abstract":"<p><p>Bone volume restoration is of particular importance when planning orthopedic treatment based on dental implants. Bone deficiency resulting from tooth loss, injuries, jaw neoplasms, or inflammatory diseases excludes the possibility of implant placement without special preparation. Autograft is the \"gold standard\" of osteoplastic material, but the limited volume and additional traumatization make it difficult to use. Prospects for the restoration of bone defects are opened by a 3D tissue-engineered osteograft obtained by direct differentiation from a chondrograft in an osteogenic medium in vitro, which is osteogenic tissue with high regenerative potential.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"547-551"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Effects of Tetrahydrobiopterine, L-Tryptophan, and Iron Ions on the Thermal Stability of Wild Type and P447R Mutant Tryptophan Hydroxylase 2. 四氢生物碱、l -色氨酸和铁离子对野生型和P447R突变体色氨酸羟化酶2热稳定性影响的比较
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI: 10.1007/s10517-025-06354-6
P D Komleva, R Deeb, E I Terentieva, A V Kulikov
{"title":"Comparison of the Effects of Tetrahydrobiopterine, L-Tryptophan, and Iron Ions on the Thermal Stability of Wild Type and P447R Mutant Tryptophan Hydroxylase 2.","authors":"P D Komleva, R Deeb, E I Terentieva, A V Kulikov","doi":"10.1007/s10517-025-06354-6","DOIUrl":"10.1007/s10517-025-06354-6","url":null,"abstract":"<p><p>The enzyme tryptophan hydroxylase 2 (TPH2) hydroxylates L-tryptophan to L-5-hydroxytryptophan (5-HTP) in the presence of tetrahydrobiopterin (BH<sub>4</sub>), iron ions (Fe<sup>2+</sup>), and molecular oxygen, the first and rate-limiting step in the synthesis of the neurotransmitter serotonin (5-HT) in the mammalian brain. We compared the effect of L-tryptophan and bi- and trivalent iron ions on the thermal stability of the mutant (mt) and wild type (wt) TPH2 molecules with those of BH<sub>4</sub>. Mt TPH2 and wt TPH2 molecules were extracted from the midbrain of BALB/c and C57BL/6 mice, respectively. Thermal stability was examined using a thermal denaturation curve and assessed by T<sub>50</sub>, the temperature at which half of the enzyme molecules are denatured. L-tryptophan at a concentration of 0.2 mM did not stabilize TPH2. BH<sub>4</sub> increased T<sub>50</sub> of the mt enzyme at concentration of 0.2 mM, but not 0.1 mM. It was shown for the first time that divalent and trivalent iron ions at concentrations of 0.01, 0.05, and 0.2 mM increased T<sub>50</sub> of the mt and wt enzyme significantly higher than BH<sub>4</sub>. The results prompt using iron ions for elucidation of the molecular mechanisms of thermal stability of TPH2 in order to create pharmacological chaperones for correcting mutation-induced disturbances in TPH2 activity and the associated psychopathologies.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"447-452"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GC-Reach DNA Fragments Reduce the Expression of Survival Genes in MCF7 Breast Carcinoma Cells: TLR9/MyD88/NF-κB Signaling Pathway as a Potential Target for Cancer Therapy. GC-Reach DNA 片段可降低 MCF7 乳腺癌细胞中存活基因的表达：TLR9/MyD88/NF-κB信号通路是癌症治疗的潜在靶点
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI: 10.1007/s10517-025-06357-3
E M Malinovskaya, G V Shmarina, E S Ershova, L V Kameneva, N N Veiko, V P Veiko, M S Konkova, P A Bobrovsky, E A Kozhina, P E Umriukhin, V N Lazarev, A Y Asanov, T M Rozhnova, V N Nikolenko, M Y Sinelnikov, S V Kostyuk
{"title":"GC-Reach DNA Fragments Reduce the Expression of Survival Genes in MCF7 Breast Carcinoma Cells: TLR9/MyD88/NF-κB Signaling Pathway as a Potential Target for Cancer Therapy.","authors":"E M Malinovskaya, G V Shmarina, E S Ershova, L V Kameneva, N N Veiko, V P Veiko, M S Konkova, P A Bobrovsky, E A Kozhina, P E Umriukhin, V N Lazarev, A Y Asanov, T M Rozhnova, V N Nikolenko, M Y Sinelnikov, S V Kostyuk","doi":"10.1007/s10517-025-06357-3","DOIUrl":"10.1007/s10517-025-06357-3","url":null,"abstract":"<p><p>Cell-free DNA (cfDNA) attracts increasing attention not only as a diagnostic tool for tumor resistance to cytostatic therapy, but also as an active participant of the tumor process. GC-rich DNA accumulates in the cfDNA pool and stimulates TLR9/MyD88/NF-κB signaling, thereby increasing the expression of genes responsible for viability of cancer cells. We studied the effect of GC-DNA on the transcriptional activity of survival genes in wild-type MCF7 cells (wt MCF7) and TLR9 gene knockout MCF7 cells (TLR9-/- MCF7). It was shown that, in contrast to wt MCF7 cell cultures, TLR9-/- MCF7 cells responded to stimulation with GC-DNA fragments by a decrease in the activity of TLR9/MyD88/NF-κB signaling cascade and a decline in survival gene expression. Our data indicate that TLR9/MyD88/NF-κB signaling cascade components may be considered as potential targets for cancer therapy.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"467-472"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of Optimal Protocol for Decellularization of Extracellular Matrix of Mesenchymal Stromal Cells of Human Umbilical Cord Tissue. 人脐带组织间充质间质细胞细胞外基质脱细胞最佳方案的选择。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI: 10.1007/s10517-025-06369-z
E S Kochetova, D K Matveeva, A E Melik-Pashayev, E R Andreeva, Yu A Romanov, L B Buravkova
{"title":"Selection of Optimal Protocol for Decellularization of Extracellular Matrix of Mesenchymal Stromal Cells of Human Umbilical Cord Tissue.","authors":"E S Kochetova, D K Matveeva, A E Melik-Pashayev, E R Andreeva, Yu A Romanov, L B Buravkova","doi":"10.1007/s10517-025-06369-z","DOIUrl":"10.1007/s10517-025-06369-z","url":null,"abstract":"<p><p>To obtain extracellular matrix (ECM) of umbilical cord multipotent mesenchymal stromal cells (UC-MSCs), six decellularization protocols were used, based on physical (freeze/thaw) and chemical (Triton-X100, CHAPS, SDS, and NP-40) methods. The quality of decellularization was assessed using phase-contrast and fluorescence microscopy, quantitative image analysis was performed as well. It was shown that protocols based on CHAPS/NH4OH and Triton X-100/NH4OH are optimal for decellularizing UC-MSC layers and provide high-quality ECM. The SDS-based protocol may be useful when certain requirements to matrix structure are specified. The presented data will be in demand for the development of biomedical products.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"535-540"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microfluidic Chip for Studying the Mechanisms of Allogeneic Progenitor Neuronal Cells Integration into a Mature Neural Network In Vitro. 异体祖神经细胞体外整合成熟神经网络机制的微流控芯片研究。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-01-01 Epub Date: 2025-02-14 DOI: 10.1007/s10517-025-06342-w
A A Gladkov, M S Zemlyanskov, Y I Pigareva, V N Kolpakov, V B Kazantsev, I V Mukhina, A S Pimashkin
{"title":"Microfluidic Chip for Studying the Mechanisms of Allogeneic Progenitor Neuronal Cells Integration into a Mature Neural Network In Vitro.","authors":"A A Gladkov, M S Zemlyanskov, Y I Pigareva, V N Kolpakov, V B Kazantsev, I V Mukhina, A S Pimashkin","doi":"10.1007/s10517-025-06342-w","DOIUrl":"10.1007/s10517-025-06342-w","url":null,"abstract":"<p><p>The formation of functional connections between cells derived from neuronal progenitor cells (NPCs) and the developed neuronal network is a crucial task in neurobiology and cellular technologies for medical applications. We developed a technique to co-culture NPCs and mature neuronal cells in a three-chamber microfluidic chip in vitro and studied the features of functional connections among cells derived from NPCs during their integration into a network of differentiated neurons. Starting from day 20 of primary culture development, a network forms from integrated NPCs, which ensures the distribution of bioelectrical activity among neurons in a manner similar to that of differentiated cells.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"387-392"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Motor Activity Restriction on Copper Content in Rats. 运动限制对大鼠铜含量的影响。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-01-01 Epub Date: 2025-02-13 DOI: 10.1007/s10517-025-06326-w
R I Zaripova, G G Yafarova, V V Andrianov, M I Sungatullina, N I Ziyatdinova, Kh L Gainutdinov, T L Zefirov
{"title":"Effect of Motor Activity Restriction on Copper Content in Rats.","authors":"R I Zaripova, G G Yafarova, V V Andrianov, M I Sungatullina, N I Ziyatdinova, Kh L Gainutdinov, T L Zefirov","doi":"10.1007/s10517-025-06326-w","DOIUrl":"10.1007/s10517-025-06326-w","url":null,"abstract":"<p><p>In rats subjected to increasing restriction of motor activity starting from day 21 days of life, copper content was evaluated by electron paramagnetic resonance (EPR) spectroscopy by analyzing the levels of copper-containing paramagnetic complexes in the heart and liver tissues. Copper content was assessed by the intensity of the characteristic EPR signal from the Cu<sup>2+</sup>-diethyldithiocarbamate complex (Cu<sup>2+</sup>-(DETC)<sub>2</sub>). It was found that after restriction of motor activity for 30 days, copper content in the heart and liver tissues increased by 2.2 and 2.9 times, respectively.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"307-310"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Patterns of Increased Cardiomyocyte Ploidy in Myocardial Hypertrophy of Various Origins. 不同来源心肌肥大中心肌细胞倍性增加的模式。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-01-01 Epub Date: 2025-02-13 DOI: 10.1007/s10517-025-06325-x
T V Sukhacheva, R A Serov, A I Kim, V P Podzolkov, L A Bockeria
{"title":"Patterns of Increased Cardiomyocyte Ploidy in Myocardial Hypertrophy of Various Origins.","authors":"T V Sukhacheva, R A Serov, A I Kim, V P Podzolkov, L A Bockeria","doi":"10.1007/s10517-025-06325-x","DOIUrl":"10.1007/s10517-025-06325-x","url":null,"abstract":"<p><p>An increase in the ploidy of cardiomyocytes (CMCs) determines the potential for their hypertrophic growth. We analyzed the changes in CMC ploidy and size of the interventricular septum in patients with hypertrophic cardiomyopathy (HCM) and tetralogy of Fallot (TF). The ploidy of CMCs in children and adult patients with HCM and children with TF was 1.5-2-fold higher than in individuals without cardiovascular pathology, and it did not change with age. The size of the CMCs was also larger by 1.3-2 times in patients with HCM and TF, compared to the control groups. However, the increase in CMCs size was more significant in HCM than in TF. The proportion of multinucleated CMCs was significantly lower in patients with HCM compared to controls and was by almost 2 times higher in patients with TF. Thus, myocardial hypertrophy in HCM is mainly due to the polyploidy of mononucleated CMCs, whereas in TF, the proportion of multinucleated CMCs increases.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"301-306"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunochemical Characteristics and Functional Activity of Monoclonal Antibodies Obtained to the Recombinant Form of Pneumolysin. 重组型溶血素单克隆抗体的免疫化学特性及功能活性。
IF 0.9 4区 医学
Bulletin of Experimental Biology and Medicine Pub Date : 2025-01-01 Epub Date: 2025-02-13 DOI: 10.1007/s10517-025-06334-w
I V Yakovleva, N F Gavrilova, E A Kurbatova, D S Vorobyev, E S Petukhova, I B Semenova, A E Zaitsev, Yu V Volokh, A Yu Leonova, A V Poddubikov
{"title":"Immunochemical Characteristics and Functional Activity of Monoclonal Antibodies Obtained to the Recombinant Form of Pneumolysin.","authors":"I V Yakovleva, N F Gavrilova, E A Kurbatova, D S Vorobyev, E S Petukhova, I B Semenova, A E Zaitsev, Yu V Volokh, A Yu Leonova, A V Poddubikov","doi":"10.1007/s10517-025-06334-w","DOIUrl":"10.1007/s10517-025-06334-w","url":null,"abstract":"<p><p>Monoclonal antibodies (mAbs) of the IgG1 isotype obtained to the full-length recombinant pneumolysin (rPly) did not recognize or poorly recognized conformational epitopes of native pneumolysin in ELISA. At the same time, polyclonal antibodies (pAbs(rPly)) detected native pneumolysin in sandwich ELISA when they were used as capture and detecting antibodies simultaneously. All mAbs(rPly) inhibited erythrocyte hemolysis induced by native pneumolysin. The combined use of mAbs(rPly) did not reveal an increase in optical density in ELISA and an increase in inhibition hemolytic activity, which suggests that mAbs(rPly) are directed to the same or spatially closely located epitopes. pAbs(rPly) more effectively inhibited erythrocyte hemolysis than mAbs(rPly).</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"346-350"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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