Bulletin of Experimental Biology and Medicine最新文献_第8页

Consequences of Reprogrammed CD8⁺ T-Cell Therapy for Lewis Lung Carcinoma Cells and Neovasculogenesis in C57BL/6 Mice. 重编程CD8+ t细胞治疗对C57BL/6小鼠Lewis肺癌细胞和新血管生成的影响

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-06 DOI: 10.1007/s10517-025-06315-z

E G Skurikhin, N N Ermakova, M A Zhukova, E S Pan, I L Zharkikh, V Yu Pan, A A Kubatiev, S G Morozov, V E Skurikhina, M Yu Minakova, O V Pershina, A M Dygai

{"title":"Consequences of Reprogrammed CD8+ T-Cell Therapy for Lewis Lung Carcinoma Cells and Neovasculogenesis in C57BL/6 Mice.","authors":"E G Skurikhin, N N Ermakova, M A Zhukova, E S Pan, I L Zharkikh, V Yu Pan, A A Kubatiev, S G Morozov, V E Skurikhina, M Yu Minakova, O V Pershina, A M Dygai","doi":"10.1007/s10517-025-06315-z","DOIUrl":"https://doi.org/10.1007/s10517-025-06315-z","url":null,"abstract":"We studied the effect of reprogrammed CD8+ T cells (rT cells) from the bone marrow of intact mice on tumor cells and neovasculogenesis in mice with orthotopic Lewis lung carcinoma (LLC). Reprogramming of T cells was carried out using a MEK inhibitor and a PD-1 blocker; the targeting of rT cells to tumor cells was achieved by preincubation with LLC cell lysate. It was shown that the antitumor effect of rT cells was based on apoptosis of tumor cells. In addition, cell therapy reduced the number of endothelial cells (CD45-CD309+) and angiogenic cell precursors (CD45-CD117+CD309+), mesenchymal stem cells (CD45-CD31-CD34-CD44+), myeloid (CD45+CD34+CD31-) and non-myeloid (CD45+CD34-CD31-) fibrocytes, and leukocytes (CD45+) in the lungs and increased their number in the blood. Thus, rT cells impaired the recruitment of neovasculogenic cells to the lung. The antitumor effects of rT cells are superior to those of naive CD8+ T cells. The proposed reprogramming method can be useful in developing effective approaches to the therapy of lung cancer, as it allows obtaining cytotoxic rT cells capable of reducing the activity of neovasculogenesis.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"244-249"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromycetes of the Genus Alternaria Are Producers of Emerging Mycotoxins: Analysis of Profile and Toxinogenic Potential In Vitro.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06310-4

L P Minaeva, Yu M Markova, I B Sedova, Z A Chaly

{"title":"Micromycetes of the Genus Alternaria Are Producers of Emerging Mycotoxins: Analysis of Profile and Toxinogenic Potential In Vitro.","authors":"L P Minaeva, Yu M Markova, I B Sedova, Z A Chaly","doi":"10.1007/s10517-025-06310-4","DOIUrl":"https://doi.org/10.1007/s10517-025-06310-4","url":null,"abstract":"Micromycetes from the genus Alternaria are commonly found in plant food raw materials, and their produced emerging mycotoxins (EMT) pose a risk to human health. Based on polyphase taxonomy, we studied the species composition of the Alternaria spp. population in samples of Russian grain and berries; non-toxinogenic species of Alternaria of the Infectoriae section and toxinogenic species of the Alternaria section were found. Using in vitro HPLC-MS/MS, a high potential for EMT production was revealed in strains from the Alternaria section: alternariol and alternariol methyl ester, tenuazonic acid, altenuene, and tentoxin. These findings indicate that species of the Alternaria section play a significant role in the contamination of plant foods with EMT. The proposed algorithm for in vitro analysis of toxin formation can be implemented in screening for toxin-producing species within the Alternaria spp. population, allowing the differentiation of chemotypes and an expansion of the understanding of the biodiversity within the Alternaria spp. population.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"218-222"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of Metabolism and Its Regulation in the Dynamics of Experimental Models of Metabolic Disorders. 代谢紊乱实验模型动力学中的代谢特征及其调控。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-06 DOI: 10.1007/s10517-025-06321-1

A V Shestopalov, E V Krolenko, A A Nedorubov, O V Borisenko, K E Popruga, V V Makarov, S M Yudin, A M Gaponov, S A Rumyantsev

引用次数: 0

Protective Effect of Acteoside against Hydrogen Peroxide-Induced Oxidative Damage in Human Corneal Epithelial Cells.

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-12-01 Epub Date: 2025-01-07 DOI: 10.1007/s10517-025-06309-x

Y Huang, Q Peng

{"title":"Protective Effect of Acteoside against Hydrogen Peroxide-Induced Oxidative Damage in Human Corneal Epithelial Cells.","authors":"Y Huang, Q Peng","doi":"10.1007/s10517-025-06309-x","DOIUrl":"https://doi.org/10.1007/s10517-025-06309-x","url":null,"abstract":"We studied the effect of acteoside on a model of human corneal epithelial cells (HCEC) injury induced by H2O2. HCEC were divided into 4 groups and cultured for 24 h in normal medium (intact and control groups, respectively), or in a medium containing DMSO or 160 μM acteoside (DMSO and acteoside groups, respectively). Then, H2O2 solution was added to HCEC for 4 h, except for intact cells. The cell viability was assessed by the CCK8 method to determine the working concentrations of acteoside and H2O2 for further experiments. Quantitative PCR and Western blotting were used to determine the effect of acteoside and H2O2 on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NADPH:quinone oxidoreductase-1 (NQO-1), and cyclooxygenase-2 (COX-2). The effects of these agents on cell proliferation were assessed by staining with 5-ethynyl-2'-deoxyuridine (EdU). It was found that acteoside protected HCEC against H2O2-induced damage by inhibiting the expression of Nrf2, HO-1, NQO-1, and COX-2.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"213-217"},"PeriodicalIF":0.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Effect of Curcumin on Cognitive Behavior and Pathological Characteristics of the Hippocampus in Mice with Inherent Alzheimer's Disease. 撤稿说明：姜黄素对固有阿尔茨海默病小鼠认知行为和海马体病理特征的影响》（Retraction Note: Curcumin on Cognitive Behavior and Pathological Characteristics of the Hippocampus in Mice with Inherent Alzheimer's Disease）。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-12 DOI: 10.1007/s10517-024-06251-4

M J Zhang, J H Zhong, X J Yi

引用次数: 0

Specific Disruption of Memory Reconsolidation of Conditioned Food Aversion in Snails by DNA Synthesis Inhibitors. DNA 合成抑制剂对蜗牛条件反射食物厌恶记忆再巩固的特异性破坏

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-01 Epub Date: 2024-11-20 DOI: 10.1007/s10517-024-06274-x

S V Solntseva, O I Efimova, V P Nikitin

{"title":"Specific Disruption of Memory Reconsolidation of Conditioned Food Aversion in Snails by DNA Synthesis Inhibitors.","authors":"S V Solntseva, O I Efimova, V P Nikitin","doi":"10.1007/s10517-024-06274-x","DOIUrl":"10.1007/s10517-024-06274-x","url":null,"abstract":"The involvement of DNA synthesis in the mechanisms of long-term memory reconsolidation in edible snails trained for conditioned food aversion was investigated. Administration of nucleoside analogs, such as 5-bromo-2'-deoxyuridine or 3'-azido-3'-deoxythymidine, which inhibit DNA synthesis, 1 h before or 1-3 h, but not 5 h after reminder with the conditioned stimulus led to memory impairment. One day after the inhibitor application and memory reactivation, a weakly expressed memory impairment (amnesia) was observed, which progressed over the next few days to the complete disappearance of behavioral memory expression. In snails with formed aversive memory for two food conditioned stimulus, a specific memory impairment was observed only for the stimulus that was paired with the presentation of an inhibitor during the reminder, while the memory for the other non-reactivated conditioned stimulus remained intact. It is suggested that DNA synthesis in the brain plays a specific role in the genetic mechanisms of reconsolidation and maintenance of long-term conditioned food aversion memory in snails.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"11-16"},"PeriodicalIF":0.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Expression of GFAP in the Brain Astrocytes of Deceased Newborns Depending on the Postmortem Interval. 死亡新生儿脑星形胶质细胞中 GFAP 的免疫组化表达取决于尸检时间间隔。

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-01 Epub Date: 2024-11-23 DOI: 10.1007/s10517-024-06291-w

A I Shchegolev, U N Tumanova, O V Savva, G T Sukhikh

{"title":"Immunohistochemical Expression of GFAP in the Brain Astrocytes of Deceased Newborns Depending on the Postmortem Interval.","authors":"A I Shchegolev, U N Tumanova, O V Savva, G T Sukhikh","doi":"10.1007/s10517-024-06291-w","DOIUrl":"10.1007/s10517-024-06291-w","url":null,"abstract":"The changes in astrocytes and their role in the development of brain diseases can be identified by morphological analysis of tissue specimens, in particular, by immunohistochemical detection of glial fibrillary acidic protein (GFAP). The study presents analysis of GFAP expression in white matter astrocytes of deceased newborns depending on the duration of the postmortem period. Autopsy material of the brain tissue obtained from 48 deceased newborns was divided into 8 groups depending on the duration of the postmortem period. Immunohistochemical analysis was performed with antibodies to GFAP in tissue samples taken from the superior and inferior brain areas relative to the position of the body stored before autopsy. The area of GFAP+ staining per field of view was determined in the white matter using an image analysis system. Morphometric analysis revealed a decrease in the mean values of the area of GFAP+ staining, i.e. the number of fibrotic astrocytes and their processes decreased with increasing the duration of the postmortem period. The mean areas of GFAP+ staining in the superior and inferior areas of the white matter did not differ significantly between the groups. The identified changes reflect the development of nonspecific postmortem changes which should be considered when taking tissue samples for molecular biological studies and in differential forensic diagnosis with lifetime lesions and diseases.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"105-109"},"PeriodicalIF":0.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Molecular-Biological Features of Lewis Lung Carcinoma Progression in Mice with Different Resistance to Hypoxia. 不同耐缺氧能力小鼠的路易斯肺癌进展的形态学和分子生物学特征

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-01 Epub Date: 2024-11-25 DOI: 10.1007/s10517-024-06301-x

D Sh Dzhalilova, M A Maiak, A M Kosyreva, M V Silina, I S Tsvetkov, O V Makarova

{"title":"Morphological and Molecular-Biological Features of Lewis Lung Carcinoma Progression in Mice with Different Resistance to Hypoxia.","authors":"D Sh Dzhalilova, M A Maiak, A M Kosyreva, M V Silina, I S Tsvetkov, O V Makarova","doi":"10.1007/s10517-024-06301-x","DOIUrl":"10.1007/s10517-024-06301-x","url":null,"abstract":"In adult male mice with high (HR) and low (LR) resistance to hypoxia, on days 21 and 28 after subcutaneous injection of Lewis lung carcinoma cells, a morphological and morphometric study of the primary tumor nodes and metastases in the lungs was carried out. Peripheral blood parameters and subpopulation composition of blood cells, the expression of genes responsible for the development of inflammation (Nfkb, Il1b, Il6, Tnfa, Il10, and Tgfb) and the response to hypoxia (Hif1a) in the liver were also assessed. The tumors were detected in 84.6% HR and 91.7% LR mice. The mitotic index of tumor cells in the subcutaneous nodes of HR animals was statistically significantly higher. The metastases area on days 21 and 28 did not differ. In animals of both groups, an increase in the absolute number of leukocytes, monocytes, and granulocytes, a decrease in the hemoglobin content and the absolute number of erythrocytes in the peripheral blood were detected on day 28 of the experiment. Only in LR animals, an increase in the absolute number of CD11b+ monocytes was found on day 28 of the experiment in comparison with the control group. The expression of Hif1a, Nfkb, Tnfa, and Tgfb genes in the liver of LR animals was higher than in HR mice, which attested to more pronounced systemic inflammatory response. These data should be taken into account when developing new approaches for the treatment of neoplastic disorders.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"169-175"},"PeriodicalIF":0.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro and In Vivo Antitumor Activities of Isothiourea and Cinnamic Acid Derivative with NOS/MCT Inhibitory Effect. 具有 NOS/MCT 抑制作用的异硫脲和肉桂酸衍生物的体外和体内抗肿瘤活性

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-01 Epub Date: 2024-11-22 DOI: 10.1007/s10517-024-06284-9

A A Shitova, M V Filimonova, O V Soldatova, I K Volkova, V A Rybachuk, L I Shevchenko, V I Surinova, A D Kaprin, P V Shegay, S A Ivanov, E V Shlyakhto, A S Filimonov

{"title":"In Vitro and In Vivo Antitumor Activities of Isothiourea and Cinnamic Acid Derivative with NOS/MCT Inhibitory Effect.","authors":"A A Shitova, M V Filimonova, O V Soldatova, I K Volkova, V A Rybachuk, L I Shevchenko, V I Surinova, A D Kaprin, P V Shegay, S A Ivanov, E V Shlyakhto, A S Filimonov","doi":"10.1007/s10517-024-06284-9","DOIUrl":"10.1007/s10517-024-06284-9","url":null,"abstract":"This work presents the method of synthesis and physicochemical characterization of isothiourea and cinnamic acid original derivative α-cyano-4-hydroxycinnamate 1-cyclohexanoy-l-2-ethylisothiourea (T1114). In studies of the cytotoxic and antitumor activity of T1114, it has been found that the combination in one molecular structure of NOS-inhibitory fragment (1-cyclohexanoyl-2-ethylisothiourea) and a fragment inhibiting monocarboxylate lactate transporters (MCT) (α-cyano-4-hydroxycinnamic acid) does not modify the cytotoxic activity of bifunctional NOS/MCT-inhibitor T1114 in vitro. But in vivo inhibition of NOS and MCT is able to realize effects on the tumor microenvironment and hypoxic tumor cells. Such structural and functional modification has significantly extended the antitumor activity of the new NOS/MCT inhibitor. The bifunctional compound not only realized a more pronounced antitumor effect, but also prevented the development of hypoxic adaptation in solid Ehrlich carcinoma and acquired the ability to overcome the resistance of mouse cervical cancer (RShM-5). Therefore, the combination of NOS-inhibitory, anti-vasculogenic and hypoxia-oriented toxic effects can create new opportunities in antiangiogenic therapy of malignant neoplasms.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"68-74"},"PeriodicalIF":0.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transplantation of Cultured Mononuclear Fraction of Bone Marrow Cells in the Treatment of Heymann Nephritis in Rats. 移植培养的骨髓单核细胞治疗大鼠海曼肾炎

IF 0.9 4区医学

Bulletin of Experimental Biology and Medicine Pub Date : 2024-11-01 Epub Date: 2024-11-22 DOI: 10.1007/s10517-024-06282-x

L P Sigareva, A A Kokorina, E V Mikhailova, S V Kromsky, P A Slizhov, V N Aleksandrov, R A Nasyrov

引用次数: 0