Bulletin of Experimental Biology and Medicine最新文献

{"title":"The Effects of Threefold 30-Minute Inhalation Sessions of an Argon-Oxygen Mixture on a Photochemically Induced Ischemic Stroke Model in Rats.","authors":"M V Sutormin, E A Boeva, D N Silachev, E I Yakupova, V V Antonova, M A Lubomydrov, S N Kalabushev, A V Erchov, O A Grebenchikov, A N Kuzovlev","doi":"10.1007/s10517-025-06427-6","DOIUrl":"10.1007/s10517-025-06427-6","url":null,"abstract":"<p><p>Studies on rats have demonstrated that successive threefold 30-min sessions of inhalation of an argon-oxygen mixture (Ar 70%/O₂ 30%) immediately after and on days 2 and 3 after ischemic stroke reduce neurological deficit, the area of vWF⁺ staining in brain sections, and the number of Iba-1⁺ and Cas-3⁺ cells in the lesion focus and NeuN⁺ cells in the penumbra. However, no differences in the lesion volume were detected by MRI. Argon-oxygen inhalations reduced neurological deficits through their influence on reparative processes within the lesion site.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"14-19"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impairment of P2 Receptor-Mediated Modulation of Skeletal Muscle Contractions in Transgenic Mice with Modeled Amyotrophic Lateral Sclerosis.","authors":"A E Khairullin, D V Efimova, A Y Teplov, A N Khabibrakhmanov, K K Nagiev, S N Grishin, A U Ziganshin, M A Mukhamedyarov","doi":"10.1007/s10517-025-06425-8","DOIUrl":"10.1007/s10517-025-06425-8","url":null,"abstract":"<p><p>We studied the effects of ATP on skeletal muscle contractility in FUS-transgenic mice with amyotrophic lateral sclerosis (ALS) model. Mechanomyography showed that ATP application did not increase the amplitude of electrically induced contractions of the diaphragm muscle, m. extensor digitorum longus, and soleus muscle in FUS-transgenic mice unlike wild-type mice. Application of a non-selective P2 receptor antagonist suramine and application of ATP against the background of suramine did not change the amplitude of contractions of the studied skeletal muscles in FUS-transgenic mice. Thus, FUS model of ALS is based on the impairment of purinergic regulation of skeletal muscle contractile activity, which can play a role in the pathogenesis of ALS.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"20-23"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of Maturation of Dendritic Cells from Monocytes Depending on HER2 Status of Breast Cancer.","authors":"A S Golderova, I E Nikolaeva","doi":"10.1007/s10517-025-06434-7","DOIUrl":"10.1007/s10517-025-06434-7","url":null,"abstract":"<p><p>In this study, we analyzed the features of maturation of dendritic cell depending on the HER2 status of breast cancer based on morphology and immunophenotyping. Monocytes were isolated from the venous blood of 12 women with breast cancer: 4 patients with HER2^- status and 8 with HER2⁺ status. In patients with HER2^- status, significantly lower (p = 0.029) expression of costimulatory membrane signal CD86 on dendritic cells on day 9 of culturing and reduced (p = 0.03) number of stretched cells on day 2 of culturing were revealed. Additionally, a correlation (r = 510; p = 0.031) was found between HER2⁺ status and the number of round immature cells on day 7 of culturing. The results indicate that maturation of dendritic cells from peripheral blood monocytes from HER2⁺ status breast cancer patients is less active and incomplete on the first and last days in culture compared to cells from HER2^- patients. Low expression of CD86 costimulatory signal can be the cause of impaired antigen-presenting function of dendritic cells, which indirectly confirms aggressiveness of HER2⁺ breast cancer.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"78-80"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Markers for Thyroid Neoplasms in the Upstream Analysis of the Plasma Proteome.","authors":"E V Bondarenko, I O Butenko, V A Ioutsi, N A Kitsilovskaya, T N Aksenova, A A Lazareva, N G Mokrysheva","doi":"10.1007/s10517-025-06432-9","DOIUrl":"10.1007/s10517-025-06432-9","url":null,"abstract":"<p><p>The reliability of existing diagnostic methods for thyroid neoplasms remains questionable, which necessitates the search for alternative approaches. The use of nontarget proteomic analysis for diagnosing oncological diseases is gaining traction and represents an efficient method for multiplex analysis. This study analyzed 372 blood plasma samples collected from patients with histologically confirmed thyroid pathologies treated at the National Medical Research Center for Endocrinology in 2019-2021. The samples were obtained prior to surgical intervention. Sample preparation involved the reduction and alkylation of disulfide bonds, followed by proteolysis and purification using specialized cartridges. Proteomic analysis was performed using nanoflow HPLC coupled with high-resolution mass spectrometry in a data-dependent acquisition mode. Peptides were identified using FragPipe software, and their suitability for biomarker discovery was assessed. From this analysis, 60 candidate proteins for thyroid disease biomarkers were identified, and sequences of 2930 peptides were evaluated. Further evaluation of nine candidate proteins revealed 31 peptides with high suitability scores for quantification. These peptides can be consolidated into a single biomarker panel that will be further developed for the risk stratification of patients with thyroid diseases.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"74-77"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmonic Analysis of Electrical Activity in Pelvic and Lumbar Regions of Healthy Men.","authors":"A D Makarov, I Yu Gavrilov, E V Limonov, E E Popov, N M Danilov, S V Revenko","doi":"10.1007/s10517-025-06435-6","DOIUrl":"10.1007/s10517-025-06435-6","url":null,"abstract":"<p><p>To noninvasively assess activity of autonomic nervous system (ANS) in pelvic as well as in left and right dorsal lumbar regions of healthy men (n = 26) in the neighborhood of prostate and kidneys, respectively, the study measured the voltaic (voltage differences) and ohmic (electrical impedances) signals. The recorded signals were processed with Fourier transform yielding the ohmic and voltaic spectral peaks in the ranges of Mayer frequency (M = 0.03-0.2 Hz) and in the Traube-Hering frequency of controlled respiration rate (TH = 0.35 Hz), which reflected activity of sympathetic and parasympathetic ANS branches, respectively. The study harvested the median and quartile values of the magnitudes of voltaic and ohmic spectral peaks at M and TH frequencies viewed as the reference values for noninvasive assessment of ANS activity in the regions of prostate and kidneys.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"86-93"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Biological Characteristics of the Cronobacter Genus Bacteria Isolated from the Dry Infant Food Products.","authors":"Yu M Markova, S A Sheveleva, N R Efimochkina, Yu V Smotrina, A S Polyanina, V V Stetsenko, I B Bykova","doi":"10.1007/s10517-025-06440-9","DOIUrl":"10.1007/s10517-025-06440-9","url":null,"abstract":"<p><p>Contamination of dry foods with the Cronobacter genus bacteria poses a significant challenge for ensuring microbiological safety of food products for infants and children, because these pathogens can cause severe infections in infants. We studied Cronobacter strains isolated from samples of dry infant food products sold in the Russian Federation, as well as components for their production. It was found that all the isolated strains belonged to the most epidemiologically significant Cronobacter sakazakii species, and the presence of at least four genes characterizing different virulence mechanisms was confirmed for all strains.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"50-53"},"PeriodicalIF":0.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Autophagy-Modulating Drugs on the Expression of Markers Associated with Cancer-Associated Fibroblasts and Epithelial-Mesenchymal Transition in a Mouse Model of Skin Melanoma.","authors":"Iu S Taskaeva, A Yu Shatruk, N P Bgatova","doi":"10.1007/s10517-025-06414-x","DOIUrl":"10.1007/s10517-025-06414-x","url":null,"abstract":"<p><p>The expression of cancer-associated fibroblast (CAF) and epithelial-mesenchymal transition marker proteins was assessed in tumor samples of B16 skin melanoma-bearing mice after chloroquine or lithium administration. Chloroquine increased the expression of CAF markers (αSMA and FAP). Hence, chloroquine can contribute to the activation of individual CAF populations in melanoma. In addition, a decrease in the expression of the epithelial marker E-cadherin and an increase in the expression of the mesenchymal marker vimentin were observed after chloroquine administration, which may indicate activation of epithelial-mesenchymal transition processes in the tumor.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"769-773"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Increased False-Positive ELISA Results for HIV in the Blood of Donors Infected with SARS-CoV-2.","authors":"H Lin, Z Wang, Q Ren, Z Cai, F Ge, W Yuan, T Liao, Y Wu, R Peng, Y Gu, Y Kuang","doi":"10.1007/s10517-025-06411-0","DOIUrl":"10.1007/s10517-025-06411-0","url":null,"abstract":"<p><p>The study examined the effect of IgG antibodies against SARS-CoV-2 virus in the blood of donors after COVID-19 on objectivity of HIV detection in the blood samples with two ELISA variants employing reagents A or B in order to select reliable kits to minimize the false-positive HIV results. Retrospectively, the quality control data of two ELISA kits with reagents A or B developed to detect HIV were analyzed using the control sera together with the results of donor blood tested with both kits, which were collected in the periods from January to December 2022 (n = 55,528) and from January to March 2023 (n = 13,837). From the blood samples obtained in 2023, all the specimens with false-positive HIV results obtained with reagent A were selected (n = 126) together with randomly taken samples of this pool with negative HIV results (n = 345). All selected samples were tested for IgG antibodies against SARS-CoV-2 virus, thereupon the correlation analysis was performed between the samples containing SARS-CoV-2 and those with HIV detected by ELISA reagent A. The data on reactivity of IgG antibodies against SARS-CoV-2 significantly correlated with the false-positive ELISA results for HIV obtained with reagent A (r = 0.451, p < 0.01). In 2023, the share of false-positive ELISA results for HIV obtained with reagent A was 0.91% (126/13,837), which was 6.06-fold greater than the corresponding value in 2022 (0.133% (74/55,528); χ² = 230.38, p < 0.01). This data echoed the SARS-CoV-2 epidemic resulting in the appearance of antibodies against the virus in the donor blood. At the same time, the number of false-positive ELISA results for HIV obtained with reagent B in 2022 (0.014% (8/55,521)) and in 2023 (0.036% (5/13,837)) were essentially smaller than those obtained with reagent A, and the results obtained with the test kit B did not significantly differ from each other for both time periods. Thus, IgG antibodies against SARS-CoV-2 in the blood of donors after COVID-19 can affect the results of HIV detection by some ELISA reagents and consequently elevate the number of false-positive analyses.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"755-759"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the Effectiveness of Bacteriophage BF1354 in the Decontamination of Chicken Meat Artificially Contaminated with an Antibiotic-Resistant Strain of Salmonella.","authors":"E R Zulkarneev, A I Laishevtsev, I A Kiseleva, O G Efimova, A V Aleshkin","doi":"10.1007/s10517-025-06409-8","DOIUrl":"10.1007/s10517-025-06409-8","url":null,"abstract":"<p><p>Salmonella, the agent causing salmonellosis, is one of the most common food pathogens in the world. Bacteriophages are now considered as the most effective and environmentally friendly method to control pathogens in various food products. We evaluated the efficacy of Salmonella phage BF1354 in decontamination of chicken fillet samples artificially contaminated with antibiotic-resistant Salmonella. Sterile chicken fillet samples (5 × 5 cm²) were artificially contaminated with Salmonella Enteritidis and then treated with bacteriophage at a concentration of 10⁹ PFU/ml. The samples were stored at 4°C for 10 days. The number of Salmonella on days 1, 6, and 10 of the experiment decreased by 2.1, 2, and 0.8 log(CFU/g), respectively, in comparison with the control. The results demonstrate the efficacy of bacteriophage BF1354 in reducing the number of Salmonella cells in chicken fillets at refrigerated storage temperatures.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"744-749"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Behavioral Disorders in Laboratory Animals Using a Model of Unpredictable Chronic Mild Stress.","authors":"V M Pavlov, V A Palikov, A Yu Fedotova, M V Shinelev, I A Pakhomova, M S Kazakova, S I Baidarova, I A Dyachenko","doi":"10.1007/s10517-025-06419-6","DOIUrl":"10.1007/s10517-025-06419-6","url":null,"abstract":"<p><p>Using the developed model of unpredictable mild chronic stress in rats, the time to the development of an anxious condition without severe depressive disorders were determined. A combination of stressors to achieve this condition is proposed: individual housing, food and water deprivation, forced swimming in cold (4°C) water, keeping on wet bedding and without bedding, under bright lighting, and at 45°C, light/dark cycle alterations, and 45° cage tilting. The condition of the animals was analyzed by changes in body weight, fructose consumption, coat state, as well as by the parameters of open field and forced swimming tests and nest building. These methods can be used in the study and search for new drugs for the treatment and prevention of anxiety- and depression-like conditions.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"794-797"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}