A A Kravtsov, O M Lyasota, E A Kozlova, A V Moiseev, S V Kozin
{"title":"培养介质中氘浓度对细胞内Ca2+水平、线粒体膜电位和葡萄糖剥夺期间神经元死亡的影响。","authors":"A A Kravtsov, O M Lyasota, E A Kozlova, A V Moiseev, S V Kozin","doi":"10.1007/s10517-025-06424-9","DOIUrl":null,"url":null,"abstract":"<p><p>We studied the effect of increasing and decreasing the deuterium level on the mitochondrial membrane potential (MMP), Ca<sup>2+</sup> homeostasis, and neuronal death in the rat cerebellar neurons in vitro. It was found that decreasing deuterium concentration to 100 and 50 ppm was accompanied by gradual depression of MMP and a decrease in the level of cytosolic Ca<sup>2+</sup> compared to the baseline values at deuterium concentration 150 ppm. Increasing deuterium concentration to 300 and 750 ppm was associated with an increase in MMP and Ca<sup>2+</sup> levels. A decrease in deuterium concentration to 100 ppm and its increase to 300 and 750 ppm reduced neuronal death after glucose deprivation. A decrease in deuterium concentration to 50 ppm produced no protective effect. Possible mechanisms for these effects are proposed.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"29-33"},"PeriodicalIF":0.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Deuterium Concentration in the Incubation Medium on Intracellular Ca<sup>2+</sup> Level, Mitochondrial Membrane Potential, and Neuronal Death during Glucose Deprivation.\",\"authors\":\"A A Kravtsov, O M Lyasota, E A Kozlova, A V Moiseev, S V Kozin\",\"doi\":\"10.1007/s10517-025-06424-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We studied the effect of increasing and decreasing the deuterium level on the mitochondrial membrane potential (MMP), Ca<sup>2+</sup> homeostasis, and neuronal death in the rat cerebellar neurons in vitro. It was found that decreasing deuterium concentration to 100 and 50 ppm was accompanied by gradual depression of MMP and a decrease in the level of cytosolic Ca<sup>2+</sup> compared to the baseline values at deuterium concentration 150 ppm. Increasing deuterium concentration to 300 and 750 ppm was associated with an increase in MMP and Ca<sup>2+</sup> levels. A decrease in deuterium concentration to 100 ppm and its increase to 300 and 750 ppm reduced neuronal death after glucose deprivation. A decrease in deuterium concentration to 50 ppm produced no protective effect. Possible mechanisms for these effects are proposed.</p>\",\"PeriodicalId\":9331,\"journal\":{\"name\":\"Bulletin of Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"29-33\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10517-025-06424-9\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06424-9","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/18 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Effect of Deuterium Concentration in the Incubation Medium on Intracellular Ca2+ Level, Mitochondrial Membrane Potential, and Neuronal Death during Glucose Deprivation.
We studied the effect of increasing and decreasing the deuterium level on the mitochondrial membrane potential (MMP), Ca2+ homeostasis, and neuronal death in the rat cerebellar neurons in vitro. It was found that decreasing deuterium concentration to 100 and 50 ppm was accompanied by gradual depression of MMP and a decrease in the level of cytosolic Ca2+ compared to the baseline values at deuterium concentration 150 ppm. Increasing deuterium concentration to 300 and 750 ppm was associated with an increase in MMP and Ca2+ levels. A decrease in deuterium concentration to 100 ppm and its increase to 300 and 750 ppm reduced neuronal death after glucose deprivation. A decrease in deuterium concentration to 50 ppm produced no protective effect. Possible mechanisms for these effects are proposed.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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