Characteristics of Sarcomere Proteins in Myocardial Sleeves of the Superior Vena Cava and Pulmonary Veins of the Porcine Heart.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-06-01 Epub Date: 2025-08-28 DOI:10.1007/s10517-025-06452-5

A M Kochurova, E A Beldiia, Yu Ya Antonets, G V Kopylova, D V Shchepkin

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Abstract

Myocardial sleeves around caval and pulmonary veins have ectopic activity, which is the main cause of atrial arrhythmias. The morphological and electrophysiological properties of this myocardium differ from those of the atria, but its mechanical activity in large animals has not been studied. We compared the phosphorylation of sarcomere proteins and the functional characteristics of myosin from the left and right atria, superior vena cava, and pulmonary veins of the porcine heart. Myosin from myocardial sleeves contains more of the β-isoform of the heavy chain, i.e., is characterized by slower kinetics of cross-bridge attachment/detachment than atrial myosin. In myocardial sleeves, phosphorylation of troponin T and I, and tropomyosin was lower than in the atria. The phosphorylation features of thin filament proteins may affect the mechanical characteristics of cardiomyocyte contraction, as well as the mechanisms, regulating myocardial contraction, such as the length-dependent regulation and the mechanoelectrical feedback.

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猪心脏上腔静脉和肺静脉心肌套管中肌节蛋白的特征。

腔静脉和肺静脉周围的心肌套管有异位活动，这是引起心房心律失常的主要原因。这种心肌的形态和电生理特性不同于心房，但其机械活性在大型动物中尚未研究过。我们比较了猪心脏左、右心房、上腔静脉和肺静脉肌球蛋白的磷酸化和功能特征。来自心肌套套的肌凝蛋白含有更多重链的β-异构体，也就是说，与心房肌凝蛋白相比，其跨桥附着/脱离动力学更慢。在心肌袖中，肌钙蛋白T、I和原肌球蛋白的磷酸化水平低于心房。细丝蛋白的磷酸化特性可能影响心肌细胞收缩的力学特性，以及调节心肌收缩的机制，如长度依赖性调节和机电反馈。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.