Archives of Virology最新文献_第5页

Isolation, identification, and genome analysis of the novel Escherichia coli phage XH12 and enhancement of the antibacterial activity of its lysozyme by chimeric cationic peptides 新型大肠杆菌噬菌体XH12的分离、鉴定、基因组分析及嵌合阳离子肽增强其溶菌酶的抗菌活性

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06274-w

Xuhao Hou, Jiaqi Pu, Yu Li, Wenhai Xie, Limei Zhang, Hongkuan Deng

{"title":"Isolation, identification, and genome analysis of the novel Escherichia coli phage XH12 and enhancement of the antibacterial activity of its lysozyme by chimeric cationic peptides","authors":"Xuhao Hou, Jiaqi Pu, Yu Li, Wenhai Xie, Limei Zhang, Hongkuan Deng","doi":"10.1007/s00705-025-06274-w","DOIUrl":"10.1007/s00705-025-06274-w","url":null,"abstract":"<div><p>Antibiotics are no longer adequate to address the threat of antibiotic resistance, especially in <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, <i>Escherichia coli</i>, and other Gram-negative pathogens that pose a serious threat to human health worldwide. The antibiotic resistance pandemic has brought about a need to search for new antimicrobials as alternatives that are effective and less prone to resistance. Phages and their lysozymes have become an attractive alternative to currently available antibiotics. However, Gram-negative bacteria have an outer membrane that acts as a strong barrier, so lysozymes are often used in combination with an outer membrane permeator or are modified to overcome the outer membrane barrier. To combat drug-resistant <i>E. coli</i>, in this study, we used the multidrug-resistant <i>E. coli</i> isolate Eco-3 as a host to isolate a lytic phage, XH12, from sewage. Phage XH12 was found to lyse 81% (30/37) of the <i>E. coli</i> isolates tested. The biological characteristics and genome sequence of phage XH12 were analyzed, and we found that lysozyme lys12 encoded by phage XH12, when combined with ethylenediaminetetraacetic acid (EDTA), exhibited antibacterial activity against <i>E. coli</i>. Two modified lysozymes were obtained by fusing cationic amino acid polypeptides to the C-terminus of lys12. The fusion lysozymes increased the antibacterial activity against <i>E. coli</i> in the extracellular space. This study of phage XH12 and its lysozyme provides basic information for further study of the treatment of multidrug-resistant <i>E. coli</i> infections.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Evolutionary analysis and population dynamics in the global transmission of Kaposi’s sarcoma-associated herpesvirus 卡波西肉瘤相关疱疹病毒全球传播的进化分析和种群动态

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06259-9

Tianye Wang, Jiaqi Wu, So Nakagawa, Takahiro Yonezawa, Zhenqiu Liu, Xin Zhang, Haili Wang, Yi Li, Tiejun Zhang

{"title":"Evolutionary analysis and population dynamics in the global transmission of Kaposi’s sarcoma-associated herpesvirus","authors":"Tianye Wang, Jiaqi Wu, So Nakagawa, Takahiro Yonezawa, Zhenqiu Liu, Xin Zhang, Haili Wang, Yi Li, Tiejun Zhang","doi":"10.1007/s00705-025-06259-9","DOIUrl":"10.1007/s00705-025-06259-9","url":null,"abstract":"<div><p>With the widespread prevalence of Kaposi’s sarcoma-associated herpesvirus (KSHV) globally, particularly in sub-Saharan Africa and the Mediterranean region, the open reading frame (ORF) K1 gene, a key gene for distinguishing different subtypes of KSHV, has been extensively studied for its diversity and sequence variations. In this study, we collected K1 gene sequences representing subtypes of KSHV worldwide in order to assess the global distribution of KSHV subtypes and to investigate the recombination and selection history of KSHV. Recombination and gene flow analysis indicated a minimum average recombination rate of 0.41 per site for the K1 gene. Recombination analysis indicated that 11 major recombination events had occurred, predominantly in subtypes A and C, while subtype B showed minimal involvement in recombination processes, consistent with the gene flow analysis. Using tip-dating methods, we estimated that the most recent common ancestor of KSHV emerged in the 12th century, while the currently globally prevalent subtypes appeared within the past three centuries. Its recent origin and rapid evolution indicate that KSHV is now undergoing strong selection and is in the process of adaptation.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-025-06259-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Puccinia triticina 小麦锈菌中鉴定的一种新型有丝分裂病毒的全基因组序列

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-27 DOI: 10.1007/s00705-025-06272-y

Yanhui Zhang, Nuoheng Wang, Jinyang Li, Bingtao Chen, Zhensheng Kang, Pengyu Song, Wenming Zheng

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Exploring evidence from cells to clinics: is human bocavirus a gastrointestinal pathogen or just a risk factor? 探索从细胞到临床的证据：人类博卡病毒是胃肠道病原体还是仅仅是一个危险因素？

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06265-x

Mehrdad Mohammadi

{"title":"Exploring evidence from cells to clinics: is human bocavirus a gastrointestinal pathogen or just a risk factor?","authors":"Mehrdad Mohammadi","doi":"10.1007/s00705-025-06265-x","DOIUrl":"10.1007/s00705-025-06265-x","url":null,"abstract":"<div><p>Human bocaviruses (HBoVs), first identified in 2005 and composed of genotypes 1–4, have been increasingly detected worldwide in pediatric patients with acute gastroenteritis. HBoV-1 has been primarily associated with respiratory symptoms, while HBoV2-4 are mostly found in gastrointestinal (GI) samples. Results from case-control studies are still controversial; however, epidemiological evidence has shown a significant association between HBoV-2 and gastroenteritis. This review will primarily focus on this association, with a brief discussion of evidence related to other HBoV genotypes. Pathological and molecular studies on the pathogenesis of HBoV, particularly in GI cells, are very scarce, possibly due to the difficulties of <i>in vitro</i> HBoV culture. Nonetheless, some relevant findings from colorectal cancer samples have yielded valuable insights regarding the behavior of HBoV in the GI system. In the present review, we provide an updated overview of the epidemiological evidence for an association of HBoV infection with acute gastroenteritis and focus on the cellular and molecular perspectives of HBoV pathogenicity. Finally, we look at the knowledge gaps about how HBoV affects the GI system and explore future directions.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and biological characterization of African swine fever virus clones selected at the early stages of adaptation in Vero cells 在Vero细胞适应的早期阶段选择的非洲猪瘟病毒克隆的遗传和生物学特性

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06269-7

Tae-Young Suh, Ji-Hoon Park, Chae-Rin Park, Ji-Eun Kim, Jun Young Park, Seong Yun Hwang, Yong-Joo Kim, Hae-Eun Kang, Dae-Yong Kim, Jun-Gu Choi

{"title":"Genetic and biological characterization of African swine fever virus clones selected at the early stages of adaptation in Vero cells","authors":"Tae-Young Suh, Ji-Hoon Park, Chae-Rin Park, Ji-Eun Kim, Jun Young Park, Seong Yun Hwang, Yong-Joo Kim, Hae-Eun Kang, Dae-Yong Kim, Jun-Gu Choi","doi":"10.1007/s00705-025-06269-7","DOIUrl":"10.1007/s00705-025-06269-7","url":null,"abstract":"<div><p>African swine fever is a highly contagious viral disease affecting domestic pigs and wild boars, caused by African swine fever virus (ASFV). ASFV exhibits highly restricted cell tropism, primarily targeting porcine macrophages for efficient and stable replication. This strict requirement for a specific cell type has posed significant challenges in ASFV research and remains a critical obstacle to vaccine development. Although several cell lines have been reported to support ASFV replication, significant genetic changes occur during passages in cell lines in unpredictable ways. In this study, we adapted a highly virulent Korean ASFV isolate to Vero cells, and plaque purification was employed during the early stages of cell adaptation to eliminate the competitive pressure between viral subpopulations. Three viral clones with distinct genetic properties were obtained and passaged independently up to 30 times. The three clones replicated efficiently in porcine alveolar macrophages (PAMs) and Vero cells even after 30 passages, while the parent virus did not grow in Vero cells. Genetic analysis revealed that the cell-adapted viral clones shared multiple single-nucleotide polymorphisms (SNPs) but also had unique SNPs and large deletions in their genome. This diversity of mutations in these clones could provide a valuable model for exploring gene functions and their effects on phenotypic properties. Future studies will focus on evaluating the phenotypic effects of these SNPs <i>in vivo</i> to support vaccine development.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of two novel bacteriophages of the class Caudoviricetes that infect Ralstonia solanacearum and Ralstonia pseudosolanacearum 描述了两种新型的茎状菌类噬菌体，它们可以感染龙舌兰菌和假龙舌兰菌

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06271-z

Beatriz Dias Januário, Rafael Reis de Rezende, Tulio Morgan, Poliane Alfenas-Zerbini

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Effectiveness of inactivated COVID-19 vaccine against symptom severity in hospitalized COVID-19 patients infected with the Omicron variant COVID-19灭活疫苗对感染欧米克隆变异的住院患者症状严重程度的影响

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-24 DOI: 10.1007/s00705-025-06275-9

Qing-Yan Ye, Zhi-Tian Jiang, Yun Jiang, Jing-Wen Cai, Zhen Zhou, Jie Song, Qian Wang, Qi-Qi Wu, Gang Zhao, Jia Chen, Qi-Hua Ling

{"title":"Effectiveness of inactivated COVID-19 vaccine against symptom severity in hospitalized COVID-19 patients infected with the Omicron variant","authors":"Qing-Yan Ye, Zhi-Tian Jiang, Yun Jiang, Jing-Wen Cai, Zhen Zhou, Jie Song, Qian Wang, Qi-Qi Wu, Gang Zhao, Jia Chen, Qi-Hua Ling","doi":"10.1007/s00705-025-06275-9","DOIUrl":"10.1007/s00705-025-06275-9","url":null,"abstract":"<div><p>In this study, we analysed the outcomes of 1165 symptomatic patients infected with the SARS-CoV-2 Omicron BA.2 variant and their response to Sinovac-CoronaVac vaccination. We assessed the effectiveness of vaccination against adverse outcomes (severe, critical, or fatal cases). Of these patients, 504 (43.3%) were men, the median age was 71 years, and 391 patients (66.4%) had received the Sinovac-CoronaVac COVID-19 vaccine (Sino Pharma, Beijing, China). The percentages of severe, critical, and fatal cases were 3.9%, 2.8%, and 3.7%, respectively, with significantly lower rates among vaccinated patients (2.8% vs. 14.2%; adjusted odds ratio [aOR], 0.306; 95% confidence interval [95% CI], 0.129–0.727). Age ≥ 70 years and a CRP level > 8 mg/L were independent predictors of an adverse outcome. Out of 630 patients aged ≥ 70 years, 107 (17.0%) were vaccinated. Seven vaccinated patients (6.5%) and 94 unvaccinated patients (18.0%) experienced adverse outcomes. Multivariate analysis indicated that vaccination (OR, 0.401; 95% CI 0.162–0.991) and CRP levels > 8 mg/L (OR, 3.262; 95% CI 1.754–6.067) were independently associated with adverse outcomes in patients aged ≥ 70 years. Inactivated vaccines were effective against symptomatic and severe COVID-19. Even in symptomatic Omicron infections, full vaccination with inactivated vaccines significantly reduced the number of adverse cases, especially in patients aged ≥ 70 years. Systemic inflammation (as measured by the CRP level) was independently correlated with adverse outcomes in patients infected with Omicron BA.2.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel gammascleroulivirus from the rice blast fungus Magnaporthe oryzae isolate ES155 来自稻瘟病真菌 Magnaporthe oryzae ES155 分离物的一种新型甘马氏硬核病毒的分子鉴定

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-23 DOI: 10.1007/s00705-025-06267-9

Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng

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The resurgence of monkeypox clade Ib: a global health emergency and concern 猴痘分支b的死灰复燃：一个全球卫生突发事件和关切

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-21 DOI: 10.1007/s00705-025-06270-0

Suresh Kumar, Gunasekaran Subramaniam, K. J. Senthil Kumar

引用次数: 0

Characterization of two novel mitoviruses co infecting a single strain of Fusarium pseudograminearum causing wheat crown rot 一株引起小麦冠腐病的伪谷粒镰刀菌感染的两种新型有丝分裂病毒的特性研究

IF 2.5 4区医学

Archives of Virology Pub Date : 2025-03-19 DOI: 10.1007/s00705-025-06242-4

Xin Pan, Yuan Xie, Shuwei Yan, Xiaoting Zhang, Xinxin Liu, Youzhou Jiao, Fei Gao

{"title":"Characterization of two novel mitoviruses co infecting a single strain of Fusarium pseudograminearum causing wheat crown rot","authors":"Xin Pan, Yuan Xie, Shuwei Yan, Xiaoting Zhang, Xinxin Liu, Youzhou Jiao, Fei Gao","doi":"10.1007/s00705-025-06242-4","DOIUrl":"10.1007/s00705-025-06242-4","url":null,"abstract":"<div><p>Wheat crown rot (WCR), caused by <i>Fusarium pseudograminearum</i>, poses a threat to wheat production worldwide. Two mycoviruses, designated as \"Fusarium pseudograminearum mitovirus 2\" (FupgMV2) and \"Fusarium pseudograminearum mitovirus 3\" (FupgMV3), were identified in <i>F. pseudograminearum</i> strain YY514-10-2. The two viral genomes are 2,429 and 2,450 nucleotides long, each containing a single open reading frame (ORF) encoding a 724-amino-acid-long RNA-dependent RNA polymerase (RdRp), with 31.98% amino acid sequence identity to each other. BLASTp analysis revealed that the RdRp of FupgMV2 exhibits at least 88.84% amino acid sequence identity to that of Fusarium mitovirus 1, while the RdRp of FupgMV3 shows 80.19% amino acid sequence identity to that of Fusarium mitovirus 2. Phylogenetic analysis indicated that FupgMV2 and FupgMV3 both belong to the genus <i>Unuamitovirus</i> of the family <i>Mitoviridae</i>. This is the first report of two mitoviruses hosted by the same strain of the plant-pathogenic fungus <i>F. pseudograminearum</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0