Archives of Virology最新文献

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Characterization of two novel mitoviruses co infecting a single strain of Fusarium pseudograminearum causing wheat crown rot 一株引起小麦冠腐病的伪谷粒镰刀菌感染的两种新型有丝分裂病毒的特性研究
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-19 DOI: 10.1007/s00705-025-06242-4
Xin Pan, Yuan Xie, Shuwei Yan, Xiaoting Zhang, Xinxin Liu, Youzhou Jiao, Fei Gao
{"title":"Characterization of two novel mitoviruses co infecting a single strain of Fusarium pseudograminearum causing wheat crown rot","authors":"Xin Pan,&nbsp;Yuan Xie,&nbsp;Shuwei Yan,&nbsp;Xiaoting Zhang,&nbsp;Xinxin Liu,&nbsp;Youzhou Jiao,&nbsp;Fei Gao","doi":"10.1007/s00705-025-06242-4","DOIUrl":"10.1007/s00705-025-06242-4","url":null,"abstract":"<div><p>Wheat crown rot (WCR), caused by <i>Fusarium pseudograminearum</i>, poses a threat to wheat production worldwide. Two mycoviruses, designated as \"Fusarium pseudograminearum mitovirus 2\" (FupgMV2) and \"Fusarium pseudograminearum mitovirus 3\" (FupgMV3), were identified in <i>F. pseudograminearum</i> strain YY514-10-2. The two viral genomes are 2,429 and 2,450 nucleotides long, each containing a single open reading frame (ORF) encoding a 724-amino-acid-long RNA-dependent RNA polymerase (RdRp), with 31.98% amino acid sequence identity to each other. BLASTp analysis revealed that the RdRp of FupgMV2 exhibits at least 88.84% amino acid sequence identity to that of Fusarium mitovirus 1, while the RdRp of FupgMV3 shows 80.19% amino acid sequence identity to that of Fusarium mitovirus 2. Phylogenetic analysis indicated that FupgMV2 and FupgMV3 both belong to the genus <i>Unuamitovirus</i> of the family <i>Mitoviridae</i>. This is the first report of two mitoviruses hosted by the same strain of the plant-pathogenic fungus <i>F. pseudograminearum</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Humoral immune response characteristics of vulnerable populations against SARS-CoV-2 strains EG.5 and JN.1 after infection with strains BA.5 and XBB 易感人群感染BA.5和XBB株后对SARS-CoV-2株EG.5和JN.1的体液免疫反应特征
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-18 DOI: 10.1007/s00705-025-06248-y
Huan Zhang, Shi Ouyang, Yunyun Qu, Zhuolin Li, Yushan Jiang, Tingting Peng, Guangyan Yang, Tao Chen, Baisheng Li, Chenguang Shen, Wei Zhao
{"title":"Humoral immune response characteristics of vulnerable populations against SARS-CoV-2 strains EG.5 and JN.1 after infection with strains BA.5 and XBB","authors":"Huan Zhang,&nbsp;Shi Ouyang,&nbsp;Yunyun Qu,&nbsp;Zhuolin Li,&nbsp;Yushan Jiang,&nbsp;Tingting Peng,&nbsp;Guangyan Yang,&nbsp;Tao Chen,&nbsp;Baisheng Li,&nbsp;Chenguang Shen,&nbsp;Wei Zhao","doi":"10.1007/s00705-025-06248-y","DOIUrl":"10.1007/s00705-025-06248-y","url":null,"abstract":"<div><p>In this study, we compared the humoral immune characteristics of children, elderly individuals, and pregnant women in Guangzhou, China, who had been infected with the SARS-CoV-2 strains BA.5 and XBB against the currently predominant strains EG.5 and JN.1. It was discovered that the neutralizing antibody titers in children, elderly individuals, and pregnant women against strains EG.5 and JN.1 were low in individuals who had been infected with strain BA.5, irrespective of their vaccination status. There was a significant positive correlation between the neutralization titers against JN.1 and EG.5 in both the acute and convalescent phases of BA.5 infection. For XBB-infected patients, the sera in the acute stage exhibited a low neutralizing titer against EG.5 and JN.1, whereas the convalescent sera demonstrated a significantly higher neutralizing titer against the two viruses, particularly in infected individuals who had been vaccinated. For XBB-infected patients, there was a strong positive correlation between the serum neutralizing antibody titers against EG.5 and JN.1 in both the acute and recovery phases. This finding provides crucial information for judging the epidemic trend of COVID-19 and the development of vaccines, especially for developing customized vaccines and immune strategies for different populations.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular characterization of a novel mycovirus from Curvularia lunata in China 中国弯孢菌一种新型分枝病毒的分子特征分析
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-17 DOI: 10.1007/s00705-025-06268-8
Bin Ke, Xiaohan Mo, Mengting Wu, Lin Wang, Ru Peng, Yuwen Lu, Jiejun Peng, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan, Hongying Zheng
{"title":"Molecular characterization of a novel mycovirus from Curvularia lunata in China","authors":"Bin Ke,&nbsp;Xiaohan Mo,&nbsp;Mengting Wu,&nbsp;Lin Wang,&nbsp;Ru Peng,&nbsp;Yuwen Lu,&nbsp;Jiejun Peng,&nbsp;Shaofei Rao,&nbsp;Guanwei Wu,&nbsp;Jianping Chen,&nbsp;Fei Yan,&nbsp;Hongying Zheng","doi":"10.1007/s00705-025-06268-8","DOIUrl":"10.1007/s00705-025-06268-8","url":null,"abstract":"<div><p>A novel mycovirus was identified in <i>Curvularia lunata</i> in China using high-throughput sequencing and RT-PCR, and the virus was named \"Curvularia lunata RNA virus 1\" (ClRV1). The genome of ClRV1 consists of 3,742 nucleotides and contains two open reading frames (ORFs). In a BLASTn search based on the complete genome sequence of ClRV1, this virus was found to have the most sequence similarity to Erysiphe necator associated umbra-like virus 4 (EnUlV4) (74% identity, 54% query coverage), and BLASTp searches showed that the ClRV1 HP and RdRp shared the highest amino acid sequence identity of 66.5% and 74.7%, respectively, with the corresponding proteins of EnUlV4. Phylogenetic analysis based on RdRp amino acid sequences indicated that ClRV1 grouped together with EnUlV4 and a number of other mycoviruses that have been variously named ‘ambiguiviruses’, ‘mycotombusviruses’, and ‘umbramycoviruses’, which appears to justify the creation of a new order within the phylum <i>Kitrinoviricota.</i> This is the first report of the complete genome sequence of the novel mycovirus ClRV1 infecting <i>Curvularia lunata</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and serological detection of maize yellow mosaic virus 玉米黄花叶病毒的纯化及血清学检测
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-14 DOI: 10.1007/s00705-025-06249-x
Jennifer R. Wilson, Kristen J. Willie, Nitika Khatri
{"title":"Purification and serological detection of maize yellow mosaic virus","authors":"Jennifer R. Wilson,&nbsp;Kristen J. Willie,&nbsp;Nitika Khatri","doi":"10.1007/s00705-025-06249-x","DOIUrl":"10.1007/s00705-025-06249-x","url":null,"abstract":"<div><p>Maize yellow mosaic virus (MaYMV) is an emerging polerovirus that was first reported in maize in China in 2013 but has since been reported in Africa and the Americas. Due to its novelty, no antibodies or serological diagnostics for the virus existed prior to the current study. In this study, we developed the first purification method for maize yellow mosaic virus with the goal of generating an antibody and subsequently developing diagnostic tests. Yields of 0.634–1.275 milligrams of virus per kilogram of maize tissue were obtained, which is comparable to the yields obtained for other grass-infecting poleroviruses. Under an electron microscope, purified virus particles appear icosahedral in shape and roughly 30 nm in diameter. A polyclonal antibody was generated against the gradient-purified virus preparation and cross-adsorbed to uninfected maize tissue. The antibody is effective for detection of MaYMV via enzyme-linked immunosorbent assay (ELISA) and western blot. The ELISA could detect virus in quantities as low as 0.395 µg and in 1:32 dilutions of infected plant extract, and the antibody showed little to no cross-reactivity with five closely related viruses. After protein gel electrophoresis and antibody detection, protein bands corresponding in size to two viral structural proteins could be seen: the coat protein of roughly 21 kDa and the readthrough protein, around 72 kDa. Finally, virus particles purified using this protocol were found to be transmissible by the primary aphid vector, <i>Rhopalosiphum maidis</i>, and to cause systemic, symptomatic infection of maize plants, indicating that the vector transmissibility and infectivity of the virus particles were preserved during purification.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata 从食用菌羊肚菌中分离的一种β内啡肽病毒的鉴定
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-14 DOI: 10.1007/s00705-025-06264-y
Ruiling Lyu, Junlin Chen, Qing Tang, Du Hai, Tun Wu, Huagang Xiao, Jiatao Xie, Yang Xiao
{"title":"Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata","authors":"Ruiling Lyu,&nbsp;Junlin Chen,&nbsp;Qing Tang,&nbsp;Du Hai,&nbsp;Tun Wu,&nbsp;Huagang Xiao,&nbsp;Jiatao Xie,&nbsp;Yang Xiao","doi":"10.1007/s00705-025-06264-y","DOIUrl":"10.1007/s00705-025-06264-y","url":null,"abstract":"<div><p>A positive-sense single-stranded RNA mycovirus was isolated from <i>Morchella sextelata</i> strain ZY-1 and designated as “Morchella sextelata endornavirus 1” (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus <i>Betaendornavirus</i> in the family <i>Endornaviridae</i>.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Im Memoriam Marc H. V. van Regenmortel (1934-2024) 纪念Marc H. V. van Regenmortel (1934-2024)
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-14 DOI: 10.1007/s00705-025-06260-2
Dion du Plessis
{"title":"Im Memoriam Marc H. V. van Regenmortel (1934-2024)","authors":"Dion du Plessis","doi":"10.1007/s00705-025-06260-2","DOIUrl":"10.1007/s00705-025-06260-2","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The final cut: how giant viruses of protists are released from their hosts’ cells 最后一关:原生生物的巨型病毒是如何从宿主细胞中释放出来的
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-13 DOI: 10.1007/s00705-025-06261-1
Isabella Luiza Martins de Aquino, Bruna Luiza Azevedo, Nidia Esther Colquehuanca Arias, Matheus Felipe dos Reis Rodrigues, Jônatas Santos Abrahão
{"title":"The final cut: how giant viruses of protists are released from their hosts’ cells","authors":"Isabella Luiza Martins de Aquino,&nbsp;Bruna Luiza Azevedo,&nbsp;Nidia Esther Colquehuanca Arias,&nbsp;Matheus Felipe dos Reis Rodrigues,&nbsp;Jônatas Santos Abrahão","doi":"10.1007/s00705-025-06261-1","DOIUrl":"10.1007/s00705-025-06261-1","url":null,"abstract":"<div><p>Viruses are the most abundant biological entities on Earth, with an estimated 10<sup>31</sup> viruses in the biosphere. These particles serve as the crucial link between viral replication cycles in different host cells, employing a variety of release mechanisms, such as cell lysis, exocytosis, and budding. Among the diverse viral groups, giant viruses have garnered significant scientific interest due to their complex particles and genomes. Giant viruses may infect amoebae and other unicellular protists, exhibiting remarkable variation in size, shape, and symmetry. They belong to the realm <i>Varidnaviria</i>, kingdom <i>Bamfordvirae</i>, and phylum <i>Nucleocytoviricota</i>. This review examines the diverse viral release strategies employed by giant viruses, highlighting the mechanisms they use to exit host cells. These include the induction of cell lysis, vesicle formation, and exocytosis, which vary not only between different species but also within individual viral groups. The diversity of release mechanisms reflects the complex evolutionary adaptations of giant viruses, providing information about their biology and life cycles.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the genetic diversity of the genera Enamovirus and Polerovirus through data-driven virus discovery 通过数据驱动的病毒发现揭示了漆包膜病毒和Polerovirus属的遗传多样性
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-13 DOI: 10.1007/s00705-025-06258-w
V. Kavi Sidharthan, Vijay Prakash Reddy, Nagendran Krishnan, B. Parameswari
{"title":"Unveiling the genetic diversity of the genera Enamovirus and Polerovirus through data-driven virus discovery","authors":"V. Kavi Sidharthan,&nbsp;Vijay Prakash Reddy,&nbsp;Nagendran Krishnan,&nbsp;B. Parameswari","doi":"10.1007/s00705-025-06258-w","DOIUrl":"10.1007/s00705-025-06258-w","url":null,"abstract":"<div><p>Enamoviruses and poleroviruses (family <i>Solemoviridae</i>) are plant-infecting icosahedral viruses that have a single-stranded RNA genome. In the present study, we examined plant transcriptome data available in the Sequence Read Archive (SRA) database and identified sequences of 22 putative novel enamo-/enamo-like and poleroviruses. The 13 putative novel enamoviruses that were identified were found in diverse plant species, including gymnospermic hosts, expanding the known genetic diversity of enamoviruses by 0.9-fold and broadening their known host range. Other significant findings of this study include the first identification of a putative polerovirus in a gymnospermic host, identification of a viral genome whose organization resembles that of an enamovirus but clusters phylogenetically with poleroviruses, identification of a phylogenetically divergent enamo-like virus, identification of a novel enamovirus and a polerovirus in the same host sampled from same geographical location, and discovery of a novel tombus-like associated RNA in a plant containing a novel enamovirus. The genome organization of the identified viruses largely resembled those of other members of these genera. Further studies are needed to investigate the properties and distribution of these viruses.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular insights from 2023 and 2024 outbreaks reveal exclusive circulation of peste des petits ruminants virus lineage IV in India 从2023年和2024年爆发的分子分析揭示了印度小反刍动物害虫病毒谱系IV的独家传播
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-13 DOI: 10.1007/s00705-025-06254-0
Asha Anand, Swathi Mahadevappa, Rakshit Ojha, Archana Pal, Shweta Priya, Annett Helcita Dsouza, Prajakta Prashant Bokade, Kirubakaran Vinod Kumar, Divakar Hemadri, Baldev Raj Gulati, Vinayagamurthy Balamurugan
{"title":"Molecular insights from 2023 and 2024 outbreaks reveal exclusive circulation of peste des petits ruminants virus lineage IV in India","authors":"Asha Anand,&nbsp;Swathi Mahadevappa,&nbsp;Rakshit Ojha,&nbsp;Archana Pal,&nbsp;Shweta Priya,&nbsp;Annett Helcita Dsouza,&nbsp;Prajakta Prashant Bokade,&nbsp;Kirubakaran Vinod Kumar,&nbsp;Divakar Hemadri,&nbsp;Baldev Raj Gulati,&nbsp;Vinayagamurthy Balamurugan","doi":"10.1007/s00705-025-06254-0","DOIUrl":"10.1007/s00705-025-06254-0","url":null,"abstract":"<div><p>Peste des petits ruminants (PPR) is a highly contagious disease affecting sheep and goats, caused by PPR virus (PPRV), a member of the genus <i>Morbillivirus</i>. In 2023 and 2024, on testing of suspected clinical samples for PPR received through passive surveillance of sheep and goat flocks in various states of India resulted in the documentation of 15 laboratory-confirmed outbreaks of PPR. During the outbreak investigation, morbidity and significant mortality rates were observed, and clinical samples collected from the affected flocks were tested using ELISA and RT-PCR, confirming the presence of PPRV. We studied in detail nine out of 15 confirmed outbreaks for characterization of virus including partial sequencing of nucleocapsid (N) and fusion (F) genes of the PPRV. In addition, the virus from each outbreak was isolated from tissue and swab samples by blind passage in Vero cells. Phylogenetic analysis based on partial N gene sequences revealed that all of the Indian isolates belonged to Asian lineage IV. This study confirms that all of the cases of PPR reported so far in India have been caused by PPRV lineage IV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic analysis identifies intracellular targets for avian coronavirus NSP10 蛋白质组学分析确定了禽流感冠状病毒NSP10的细胞内靶标
IF 2.5 4区 医学
Archives of Virology Pub Date : 2025-03-13 DOI: 10.1007/s00705-025-06255-z
Hao Dong, Xueyan Li, Shengkui Xu, Yuxin Wang, Ting Xia, Peng Li, Wenke Ruan
{"title":"Proteomic analysis identifies intracellular targets for avian coronavirus NSP10","authors":"Hao Dong,&nbsp;Xueyan Li,&nbsp;Shengkui Xu,&nbsp;Yuxin Wang,&nbsp;Ting Xia,&nbsp;Peng Li,&nbsp;Wenke Ruan","doi":"10.1007/s00705-025-06255-z","DOIUrl":"10.1007/s00705-025-06255-z","url":null,"abstract":"<div><p>Avian coronavirus, also known as infectious bronchitis virus (IBV), is the causative agent of infectious bronchitis (IB). The non-structural proteins (NSPs) of IBV are critical for viral replication and for evading the host’s immune response. The innate immune response serves as the first line of defense against viral infections. The IBV genome codes for 15 NSPs (NSP2-16). In this study, we identified host proteins interacting with IBV NSP10 using co-immunoprecipitation (Co-IP) and liquid chromatography-tandem mass spectrometry (LC/MS/MS). Proteomic analysis revealed that interactions of host proteins with NSP10 are involved in processes such as localization, transport, and metabolism, regulation of the cell cycle, and antiviral responses. We further explored the role of NSP10 in these immune and cellular regulation pathways and also confirmed the interaction between NSP10 and the host protein hnRNPA1. Further investigation showed that hnRNPA1 inhibited IBV replication. It is speculated that the binding of hnRNP A1 to NSP10 interferes with the function of the replication complex, thereby inhibiting virus replication. However, co-overexpression of NSP10 and hnRNP A1 partially restored viral replication, suggesting a complex relationship between these two proteins. These findings demonstrate that IBV NSP10 plays a significant role in viral infection and in modulating host cell processes, highlighting its potential as a target for therapeutic interventions.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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