Archives of Virology最新文献_第5页

Molecular identification of a putative novel varicosavirus in Agastache rugosa in China 中国姬松茸中一种推定的新型变异病毒的分子鉴定。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-10-04 DOI: 10.1007/s00705-024-06141-0

Caixia Yang, Lei Yang, Yujie Li, Xiaoling Cui, Xue Bai, Song Zhang, Mengji Cao

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Comparative study of the propagation and plaque titration conditions for human coronavirus OC43 as a surrogate for SARS-CoV-2 以人类冠状病毒 OC43 作为 SARS-CoV-2 代用品的繁殖和斑块滴定条件比较研究。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-10-04 DOI: 10.1007/s00705-024-06146-9

Nattamon Niyomdecha, Chompunuch Boonarkart, Songkran Thongon, Prasert Auewarakul

{"title":"Comparative study of the propagation and plaque titration conditions for human coronavirus OC43 as a surrogate for SARS-CoV-2","authors":"Nattamon Niyomdecha, Chompunuch Boonarkart, Songkran Thongon, Prasert Auewarakul","doi":"10.1007/s00705-024-06146-9","DOIUrl":"10.1007/s00705-024-06146-9","url":null,"abstract":"<div>The ongoing COVID-19 pandemic is threatening human health globally. The development of effective drugs and vaccines against SARS-CoV-2 is hindered by the limited access to high-biosafety-level facilities. Although human coronavirus (HCoV) OC43, a low-pathogenic endemic human coronavirus, has been used as a surrogate virus for SARS-CoV-2 research, a standard technique for HCoV-OC43 culture and plaque titration has not been established. Our objective was to establish optimized culture and titration protocols for HCoV-OC43. The growth kinetics and permissibility to HCoV-OC43 infection of seven different cell lines were examined concurrently at two different temperatures, 33°C and 37°C. Cell lines exhibiting a cytopathic effect (CPE) were selected for plaque titration. No significant difference in the rate of cell growth was observed at the two temperatures tested. Interestingly, HCoV-OC43 was found not to be a high-temperature-sensitive virus, since it grew well at 37°C. Although RD, LLC-MK2, MRC-5, and HCT-8 cell lines supported virus growth with an obvious cytopathic effect and a high yield of virus after two days of infection, only RD cells were suitable for producing countable plaques. The incubation of the cells with 1.2% low-viscosity Avicel as an overlay medium at 37°C for 4 days appeared to promote clearer and sharper plaque morphology. However, further optimization of the plaque titration protocol is still required due to the continued observation of plaque size variation and hazy zones. We propose a cost-effective protocol for HCoV-OC43 culture and plaque titration that can be implemented at a standard conventional temperature without the need for additional special equipment.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surge of acute gastroenteritis outbreaks due to rising norovirus GII.4 transmission in Seoul childcare centers and kindergartens in 2022 compared to 2019–2021 与 2019-2021 年相比，2022 年首尔托儿所和幼儿园的诺如病毒 GII.4 传播率上升，导致急性肠胃炎疫情激增。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-27 DOI: 10.1007/s00705-024-06123-2

Euncheol Son, Young-Hoon Kim

{"title":"Surge of acute gastroenteritis outbreaks due to rising norovirus GII.4 transmission in Seoul childcare centers and kindergartens in 2022 compared to 2019–2021","authors":"Euncheol Son, Young-Hoon Kim","doi":"10.1007/s00705-024-06123-2","DOIUrl":"10.1007/s00705-024-06123-2","url":null,"abstract":"<div>Noroviruses are the most common cause of acute gastroenteritis (AGE) outbreaks in childcare centers and kindergartens. Their high transmissibility is partly due to their genetic diversity. AGE outbreaks that occurred in Seoul childcare centers and kindergartens from 2019 to 2022 were investigated, and 68 epidemiological reports prepared by public health centers in Seoul were used for data collection. In the three quarters of 2022, there were 40 outbreaks of AGE in Seoul childcare centers and kindergartens, which exceeded the 35 total outbreaks that occurred during the previous three years. The proportion of childcare centers and kindergartens with AGE outbreaks among all facilities in Seoul increased from 12.6% in 2019 to 58.8% in 2022. Noroviruses were the most common pathogens responsible for AGE outbreaks in these cases. From 2019 to 2021, norovirus GII.2 was the predominant genotype, and GII.4 was detected in about 25% of cases. However, in 2022, GII.4 became predominant and was detected in about 79% of cases. The attack rate and infection source of AGE outbreaks from 2019 to 2021 were not significantly different from those in 2022. In conclusion, the number of AGE outbreaks in Seoul childcare centers and kindergartens increased significantly, primarily because of increased norovirus GII.4 transmission in 2022.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in the genes encoding RLR and TLR3 and CMV DNAemia in subjects coinfected with human immunodeficiency virus and cytomegalovirus 人类免疫缺陷病毒和巨细胞病毒双重感染者中编码 RLR 和 TLR3 基因的多态性与 CMV DNA 血症。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-27 DOI: 10.1007/s00705-024-06114-3

Agnieszka Jabłońska, Elżbieta Jabłonowska, Mirosława Studzińska, Juliusz Kamerys, Edyta Paradowska

{"title":"Polymorphisms in the genes encoding RLR and TLR3 and CMV DNAemia in subjects coinfected with human immunodeficiency virus and cytomegalovirus","authors":"Agnieszka Jabłońska, Elżbieta Jabłonowska, Mirosława Studzińska, Juliusz Kamerys, Edyta Paradowska","doi":"10.1007/s00705-024-06114-3","DOIUrl":"10.1007/s00705-024-06114-3","url":null,"abstract":"<div>Cytomegalovirus (CMV) is a pathogen that is common worldwide and is often present in individuals infected with human immunodeficiency virus (HIV). Pattern recognition receptors (PRRs) are host sensors that activate the immune response against infectious agents. However, it is unclear whether PRR single-nucleotide polymorphisms (SNPs) are associated with the occurrence of CMV DNAemia in subjects coinfected with HIV and CMV. HIV/CMV-coinfected patients with and without CMV DNAemia were recruited for this study. The DDX58 rs10813831 and IFIH1 (rs3747517 and rs1990760) polymorphisms were genotyped using the TaqMan Allelic Discrimination Assay, whereas the DDX58 rs12006123 and TLR3 (rs3775291 and rs3775296) SNPs were analyzed using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. A mutation present in at least one allele of the DDX58 rs12006123 SNP occurred at least two times more frequently in HIV/CMV-coinfected patients with CMV DNAemia than in coinfected subjects without CMV DNAemia (OR, 2.50; 95% CI, 1.33–4.68; p = 0.004, in the dominant model). A higher level of CMV DNAemia was observed in subjects who had the heterozygous (GA) or homozygous recessive (AA) genotype for the DDX58 rs12006123 SNP compared with those who had the wild-type (GG) genotype (p = 0.0003). Moreover, in subjects with a mutation detected in at least one allele of the DDX58 rs12006123 SNP, a lower serum IFN-β concentration was found compared with those who had a wild-type (GG) genotype for this polymorphism (p = 0.024). The DDX58 rs12006123 SNP is associated with CMV DNAemia in HIV/CMV-coinfected patients.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of novel viruses associated with Olea europaea L. in South Africa 南非油橄榄相关新型病毒的基因组特征。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-27 DOI: 10.1007/s00705-024-06132-1

David A. Read, Gerhard Pietersen, Bernard Slippers, Emma Steenkamp

引用次数: 0

Prospects for the use of viral proteins for the construction of chimeric toxins 利用病毒蛋白制造嵌合毒素的前景。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-26 DOI: 10.1007/s00705-024-06139-8

D. V. Novikov, E. A. Vasilchikova, P. I. Vasilchikov

引用次数: 0

Hemagglutinin and neuraminidase of a non-pathogenic H7N7 avian influenza virus coevolved during the acquisition of intranasal pathogenicity in chickens 非致病性 H7N7 禽流感病毒的血凝素和神经氨酸酶在鸡获得鼻内致病性的过程中共同进化。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-22 DOI: 10.1007/s00705-024-06118-z

Takaya Ichikawa, Takahiro Hiono, Masatoshi Okamatsu, Junki Maruyama, Daiki Kobayashi, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda

{"title":"Hemagglutinin and neuraminidase of a non-pathogenic H7N7 avian influenza virus coevolved during the acquisition of intranasal pathogenicity in chickens","authors":"Takaya Ichikawa, Takahiro Hiono, Masatoshi Okamatsu, Junki Maruyama, Daiki Kobayashi, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda","doi":"10.1007/s00705-024-06118-z","DOIUrl":"10.1007/s00705-024-06118-z","url":null,"abstract":"<div>Polybasic amino acid residues at the hemagglutinin (HA) cleavage site are insufficient to induce the highly pathogenic phenotype of avian influenza viruses in chickens. In our previous study, an H7N7 avian influenza virus named “Vac2sub-P0”, which is nonpathogenic despite carrying polybasic amino acids at the HA cleavage site, was passaged in chick air sacs, and a virus with high intravenous pathogenicity, Vac2sub-P3, was obtained. Intranasal infection with Vac2sub-P3 resulted in limited lethality in chickens; therefore, in this study, this virus was further passaged in chicken lungs, and the resultant virus, Vac2sub-P3L4, acquired high intranasal pathogenicity. Experimental infection of chickens with recombinant viruses demonstrated that mutations in HA and neuraminidase (NA) found in consecutive passages were responsible for the increased pathogenicity. The HA and NA functions of Vac2sub-P3L4 were compared with those of the parental virus in vitro; the virus growth at 40 °C was faster, the binding affinity to a sialic acid receptor was lower, and the rate of release by NA from the cell surface was lower, suggesting that these changes enabled the virus to replicate efficiently in chickens with high intranasal pathogenicity. This study demonstrates that viruses that are highly pathogenic when administered intranasally require additional adaptations for increased pathogenicity to be highly lethal to intranasally infected chickens.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of lima bean endornavirus 1: a putative new member of the genus Alphaendornavirus (family Endornaviridae) 利马豆角病毒 1 的完整基因组序列：阿尔法豆角病毒属（豆角病毒科）的推定新成员。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-21 DOI: 10.1007/s00705-024-06135-y

Nina Aboughanem-Sabanadzovic, Rodrigo A. Valverde, Sead Sabanadzovic

{"title":"Complete genome sequence of lima bean endornavirus 1: a putative new member of the genus Alphaendornavirus (family Endornaviridae)","authors":"Nina Aboughanem-Sabanadzovic, Rodrigo A. Valverde, Sead Sabanadzovic","doi":"10.1007/s00705-024-06135-y","DOIUrl":"10.1007/s00705-024-06135-y","url":null,"abstract":"<div>In this study, we completely sequenced the genome of a new member of the genus Alphaendornavirus, family Endornaviridae, from lima bean (Phaseolus lunatus), for which we propose the name \"lima bean endornavirus 1\" (LbEV1). The complete genome of LbEV1 consists of 15,265 nucleotides, including a stretch of 12 cytosine residues at its 3’ end, and contains a long single open reading frame (ORF) coding for a 4980-aa-long polyprotein. Analysis of the polyprotein sequence revealed the presence of four conserved functional domains (in order from the N- to C-terminus): viral helicase 1, peptidase _C97, glycosyltransferase_GTB-type, and viral RNA-dependent RNA polymerase (RdRP). The LbEV1 polyprotein showed the highest amino acid sequence similarity (63% identity and 98% coverage) to Phaseolus vulgaris endornavirus 3 (PvEV3) and also showed 42% identity (95% coverage) to Geranium carolinianum endornavirus. Phylogenetic analysis based on the viral RdRp domain showed that LbEV1 belongs to a subclade within the genus Alphaendornavirus that includes three other viruses infecting plants of the genus Phaseolus.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial - The importance of porcine viruses to human health - a collection 社论 - 猪病毒对人类健康的重要性 - 文集。

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-21 DOI: 10.1007/s00705-024-06136-x

Tim Skern

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Characterization of the novel bequatrovirus vB-BcgM and its antibacterial effects in food matrices 新型贝类病毒 vB-BcgM 的特征及其在食品基质中的抗菌作用

IF 2.5 4区医学

Archives of Virology Pub Date : 2024-09-19 DOI: 10.1007/s00705-024-06134-z

Yunfei Gong, Lin Sun, Xiaofu Wan, Peiling Geng, Xiaomin Hu

{"title":"Characterization of the novel bequatrovirus vB-BcgM and its antibacterial effects in food matrices","authors":"Yunfei Gong, Lin Sun, Xiaofu Wan, Peiling Geng, Xiaomin Hu","doi":"10.1007/s00705-024-06134-z","DOIUrl":"10.1007/s00705-024-06134-z","url":null,"abstract":"<div>Bacteria belonging to the Bacillus cereus group are ubiquitous in nature, causing food spoilage and food poisoning cases. A bequatrovirus, vB-BcgM, belonging to the C3 cluster infecting B. cereus group members, was isolated and characterized. Its 160-kb linear dsDNA genome contains a number of replication-related coding sequences (CDSs) and displays a collinear relationship with that of the virulent phage B4, with variations in its structural and replication regions. vB-BcgM has a relatively broad host range, with the ability to infect 33.3% of the B. cereus group isolates tested, including B. cereus, B. thuringiensis, B. anthracis, B. paranthracis, B. mycoides, and B. cytotoxicus. Moreover, vB-BcgM displays efficient infection and high replication capacity. It was found that 96.5% of the virions complete the adsorption process within 5 min. The optimal multiplicity of infection (MOI) is 10-7, and the burst size is 63 plaque-forming units (PFU)/cell. This phage showed stability over a broad pH range (4-12) and at temperatures up to 70 °C. Furthermore, vB-BcgM displays significant antibacterial effects in processed food matrices (ultra-high temperature [UHT] sterilized milk [GB 25190], UHT refrigerated milk [GB 25190], pasteurized milk [GB 19645], mashed meat, and cereals) and fresh foods (lettuce, apple, and potato). The antibacterial effects were found to be dependent on the dose of viral inoculum, incubation conditions (food matrix and temperature), and time. The data indicate that vB-BcgM has good potential as an antibacterial agent.</div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142255672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0