Archives of Virology最新文献

{"title":"Frequent detection of high-risk HPV types other than HPV 16/18 in a cohort of French women who claimed to have been vaccinated","authors":"Sandy Gross,&nbsp;Louise Dalphin,&nbsp;Fanny Dor,&nbsp;Rajeev Ramanah,&nbsp;Marie-Paule Algros,&nbsp;Jean-Luc Prétet,&nbsp;Quentin Lepiller","doi":"10.1007/s00705-026-06722-1","DOIUrl":"10.1007/s00705-026-06722-1","url":null,"abstract":"<div><p>This study aimed to estimate the prevalence and distribution of HPV in a cohort of vaccinated French women undergoing cervical cancer screening (CCS). Women vaccinated against HPV (either by the bivalent HPV16/18, the quadrivalent HPV6/11/16/18 or the nonavalent HPV6/11/16/18/31/33/45/52/58 vaccine) and for whom a cervical specimen had been collected were included. Detection and genotyping of 32 alpha-HPV genotypes (HPV6/11/16/18/26/31/33/35/39/40/42/43/44/45/51/52/53/54/56/58/59/61/62/66/67/68/70/73/81/82/83/89) was systematically performed (INNO-LIPA, Fujirebio). In a sub-study, high-risk HPV (hrHPV) was detected using two different HPV screening tests, according to the study period. 464 vaccinated women were included. Cytology was normal for 80.6% of them. The prevalence of alpha-HPV was 59.9% in vaccinated women and 25% of whom had multiple infections. The most prevalent hrHPV genotypes were HPV52 (8.2%), HPV51 (6.9%), and HPV56 (5.8%). HPV16 was detected in only 4.3% of women and was present in three of the four women with a high-grade lesion. In the sub-study, 238 women underwent hrHPV screening. The prevalence of hrHPV ranged from 27.9% to 57.1%, depending on the study period and CCS practices. Alpha-HPV, including high-risk HPV, are frequently detected in vaccinated French women. However, most of the genotypes that have been detected are not covered by the vaccines. The prevalence of HPV 16/18 is low, which confirms the efficacy of HPV vaccines. The implementation of the nonavalent vaccine may change the future epidemiology of hrHPV genotypes.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid antiviral screening assay based on GFP reporter virus of bovine enterovirus","authors":"Yao Zhang,&nbsp;Xuwen Li,&nbsp;Huochun Yao,&nbsp;Chengyuan Ji,&nbsp;Zihao Pan","doi":"10.1007/s00705-026-06707-0","DOIUrl":"10.1007/s00705-026-06707-0","url":null,"abstract":"<div><p>In recent years, bovine enterovirus (BEV) has been increasingly associated with diarrhea in cattle in China, posing new challenges for disease control in the cattle industry. However, the mechanisms underlying BEV pathogenesis and virulence remain poorly understood. Infectious cDNA clones provide a powerful tool for dissecting viral replication and pathogenic mechanisms. In this study, we generated a full-length infectious cDNA clone of the BEV-F isolate HB19-1. Three overlapping fragments spanning the complete viral genome were amplified by RT-PCR and assembled downstream of a cytomegalovirus (CMV) promoter placed immediately upstream of the 5’ untranslated region (5’UTR). To establish a reporter virus system, the green fluorescent protein (GFP) gene was inserted between the 5’UTR and the N terminus of VP4, followed by a 2A cleavage sequence (IKTAG) at the C terminus of GFP. The recombinant rHB19-GFP virus was successfully rescued. Growth curve analysis demonstrated that rHB19-GFP exhibited slower replication kinetics at early time points relative to the parental HB19-1 virus, with no significant difference in their peak viral titers. This GFP-expressing reporter virus enables convenient monitoring of BEV replication and provides a useful platform for antiviral screening. Using this system, we found that 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) inhibited BEV replication, suggesting its potential as an antiviral candidate. Overall, the rHB19-GFP infectious clone developed here offers a practical tool for studying BEV biology and for identifying antiviral compounds against BEV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human pegivirus 1 in healthy blood donors and in acute febrile patients from Mato Grosso, Midwestern Brazil","authors":"Eduarda Pavan,&nbsp;Millena Moreira Carrasco,&nbsp;Janeth Aracely Ramirez Pavón,&nbsp;Leonardo Marin,&nbsp;Márcio Roberto Teixeira Nunes,&nbsp;Marcelo Adriano Mendes dos Santos,&nbsp;Renata Dezengrini","doi":"10.1007/s00705-026-06720-3","DOIUrl":"10.1007/s00705-026-06720-3","url":null,"abstract":"<div><p>The association between Human Pegivirus 1 (HPgV-1, <i>Pegivirus hominis</i>, family <i>Hepaciviridae</i>) with disease etiology remains unclear, yet this virus is widely distributed among people exposed to contaminated blood. This study aimed to identify the prevalence and characterize HPgV-1 genomes obtained from acute febrile patients (n = 92 from 2019) and from blood donors (n = 633 from 2022) sampled in Mato Grosso, Brazil. The serum samples were tested by RT-qPCR for a conserved 5’ UTR region of HPgV-1. Nucleic acid of 28 positive samples was converted to ds-cDNA, purified and sequenced with an Illumina NextSeq platform. The prevalence of HPgV-1 among acute febrile patients was 4.35% (4/92), who were mostly female (3/4; 75.00%) mean 28.86 (12–67) years-old; whereas among blood donors it was 3.79% (24/633), which were predominantly male (16/24; 66.67%), aged 30–45 years-old (13/24; 54.17%). Eight HPgV-1 genomic sequences (7,708-9,248) were recovered; two belong to subgenotype 2a and clustered with strains from United States, Brazil, France and Japan, while the remaining six genomes from subgenotype 2b grouped with strains from Pará, Brazil and abroad. HPgV-1 detection in acute febrile patients and in healthy blood donors highlights the necessity to investigate the epidemiological aspects of this infection, as well as the possible impact to public health in Midwestern Brazil.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06720-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome characterization of two novel mitoviruses and a negative-sense single-stranded RNA mycovirus from the phytopathogenic fungus Clarireedia jacksonii","authors":"Kohei Sakuta,&nbsp;Tae Mabuchi,&nbsp;Syun-ichi Urayama,&nbsp;Osamu Netsu,&nbsp;Tsutomu Arie,&nbsp;Hiromitsu Moriyama,&nbsp;Ken Komatsu","doi":"10.1007/s00705-026-06719-w","DOIUrl":"10.1007/s00705-026-06719-w","url":null,"abstract":"<div><p><i>Clarireedia jacksonii</i> is a phytopathogenic fungus responsible for dollar spot disease in turfgrass worldwide. In this study, we characterized the complete genome sequences of three novel mycoviruses isolated from <i>C. jacksonii</i> isolate MBCT-836 using next-generation sequencing and the fragmented and primer-ligated dsRNA sequencing (FLDS) method. Two of these viruses, designated Clarireedia jacksonii mitovirus 1 (CjMV1) and Clarireedia jacksonii mitovirus 2 (CjMV2), possess positive-sense single-stranded RNA genomes of 2,575 bp and 2,856 bp, respectively. Both viruses contain a single open reading frame that utilizes the mitochondrial genetic code and encodes an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis placed CjMV1 and CjMV2 within the genera <i>Unuamitovirus</i> and <i>Duamitovirus</i>, respectively, in the family <i>Mitoviridae</i>. The third virus, Clarireedia jacksonii negative-stranded RNA virus 1 (CjNSV1), features a bisegmented negative-sense RNA genome consisting of a large segment (7,961 nt) encoding an RdRp with a conserved Bunya_RdRp domain, and a small segment (1,444 nt) encoding a protein showing homology to bunyavirus nucleocapsid proteins. Phylogenetic analysis revealed that CjNSV1 clusters with members of the proposed family Sclerobunyaviridae within the order <i>Bunyavirales</i>. To our knowledge, this study provides the first report of complete genome sequences of mycoviruses infecting <i>C. jacksonii</i>, expanding our understanding of the mycovirosphere in economically significant turfgrass pathogens.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of the first alphahypovirus from the phytopathogenic fungus Curvularia americana on Poa pratensis in China","authors":"Yibo Wang,&nbsp;Miaomiao Wang,&nbsp;Yunfan Tian,&nbsp;Xuehong Wu,&nbsp;Can Zhao","doi":"10.1007/s00705-026-06721-2","DOIUrl":"10.1007/s00705-026-06721-2","url":null,"abstract":"<div><p>In the present study, a novel positive-sense single-stranded RNA virus was identified from <i>Curvularia americana</i> strain 32 − 2, a phytopathogenic fungus infecting <i>Poa pratensis</i> in China, which was designated as “Curvularia americana hypovirus 1” (CaHV1). The complete genome of CaHV1 consists of 14,386 nucleotides (nt) with a single large open reading frame (ORF) (nt position 593 to 14035). The ORF encodes a putative polyprotein (4,480 amino acids) containing two potential conserved domains, which includes a Domain of Unknown Function 3525 (DUF3525) and Helicase. BLASTp analysis showed that amino acid sequence of the polyprotein of CaHV1 shared the highest amino acid sequence identity with that of Bipolaris oryzae hypovirus 1 (63.52%). Phylogenetic analysis based on amino acid sequence of the polyprotein indicated that CaHV1 clustered together with members of the genus <i>Alphahypovirus</i> within the family <i>Hypoviridae</i>. To the best of our knowledge, this is the first report of an alphahypovirus infecting <i>C. americana</i>. </p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection and partial ORF1 characterization of psittacine beak and feather disease virus (PBFDV) in domesticated parrots of Northern Vietnam","authors":"Hoang Minh,&nbsp;Brian Harvey Avanceña Villanueva,&nbsp;Quoc Khanh Dam,&nbsp;Lai Thi Lan Huong,&nbsp;Son Vu Nguyen,&nbsp;Dang Van Tan,&nbsp;Kuo-Pin Chuang","doi":"10.1007/s00705-026-06715-0","DOIUrl":"10.1007/s00705-026-06715-0","url":null,"abstract":"<div><p>Psittacine beak and feather disease virus (PBFDV), currently classified within the species <i>Circovirus parrot</i>, is an infectious agent in avian species, particularly psittacine birds. PBFDV is the causative agent of psittacine beak and feather disease (PBFD), leading to feather loss, deformed beaks and nails, immunosuppression, and high mortality. However, there is limited information on PBFDV in Vietnam, particularly in domesticated parrots. This study aims to detect and molecularly characterize PBFDV in psittacine birds in Northern Vietnam. Among the 193 psittacine birds tested by conventional PCR, 48 were PBFDV-positive, corresponding to a positivity rate of 24.87%. Based on partial ORF1 characterization, phylogenetic tree analysis of the ten PBFDV sequences showed that genotype I, which exhibits genetic diversity, is circulating in Vietnam. This study highlights the importance of genomic characterization of PBFDV in domesticated exotic psittacine birds in Vietnam. Together with the recent cases of PBFD in domestic parrots, further surveillance is needed to elucidate the host specificity, transmission, and pathophysiology of PBFDV. </p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary infection with a Brazilian chikungunya virus isolate protects immunocompetent mice against detectable viremia following homologous rechallenge","authors":"Naiara Soares da Silva,&nbsp;Mayanna Moreira Costa Fogaça,&nbsp;Elmo José dos Santos,&nbsp;Bruna Santos Souza da Silva,&nbsp;Ruth Dálety da Silva Brito,&nbsp;Rodrigo Lucas Ferreira do Carmo,&nbsp;Amanda da Rocha Santos,&nbsp;Milena Silva Souza,&nbsp;Tarcisio Macedo Silva,&nbsp;Eric Roberto Guimarães Rocha Aguiar,&nbsp;Alexander Birbrair,&nbsp;Paloma Oliveira Vidal,&nbsp;Jaime Henrique Amorim","doi":"10.1007/s00705-026-06724-z","DOIUrl":"10.1007/s00705-026-06724-z","url":null,"abstract":"<div><p>Chikungunya virus (CHIKV) is an arthropod-borne virus responsible for an acute febrile illness characterized by severe polyarthralgia, predominantly affecting peripheral joints. In a substantial proportion of patients, these symptoms may progress to chronic manifestations that significantly impair quality of life. Animal models are therefore critical for the development of effective therapeutic and preventive strategies, including antiviral agents and vaccines. In this context, the aim of this study was to establish an experimental model of CHIKV infection using immunocompetent C57BL/6 mice. Animals were infected with CHIKV and subsequently monitored for clinical signs, viremia, viral dissemination across tissues, total leukocyte counts, and susceptibility to reinfection. Results revealed no deaths among the animals during the experimental period, and infected animals exhibited significant viremia as well as detectable viral loads in the popliteal lymph node, femoral bone marrow, and muscle, demonstrating CHIKV capacity to disseminate and reach multiple target tissues. In addition, a global reduction in leukocyte counts was observed in infected mice. Notably, animals previously infected with CHIKV were resistant to reinfection, indicating the establishment of a protective immune response, as evidenced by the presence of neutralizing antibodies. In conclusion, the murine model established in this study recapitulates key features of CHIKV infection and provides a robust platform for investigating viral pathogenesis, as well as for evaluating novel antiviral strategies and vaccine candidates.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06724-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation of wild-type rabies virus on vaccine producing cell line and its quantification by peroxidase-based immunostaining","authors":"Muhammad Rehan Riaz,&nbsp;Syed Muhammad Kamran Ullah,&nbsp;Muhammad Rashid,&nbsp;Kaleem Ullah,&nbsp;Hamid Ahmad,&nbsp;Qari Muhammad Kaleem,&nbsp;Faiza Jamil,&nbsp;Saifullah Khan","doi":"10.1007/s00705-026-06717-y","DOIUrl":"10.1007/s00705-026-06717-y","url":null,"abstract":"<div><p><i>Rabies lyssavirus</i>, the causative agent of rabies (acute progressive encephalomyelitis) requires specific receptors on host cell to mediate neurotropism. Typically, cell lines used for rabies vaccine production are devoid of these receptors. In this study, we developed and optimized a protocol for adapting a locally isolated wild type rabies virus belonging to the Arctic-like lineage A1a, to a vaccine producing cell line, quantified its infectious titer using peroxidase-based immunostaining and virus neutralization was assessed using sera obtained from Abhayrab^®-vaccinated individuals. The virus was initially propagated in mouse neuroblastoma cell line and gradually adapted to replicate in Vero E6 by serial passages. The adaptation was confirmed by cytopathic effect and the viral titer rose to as high as 1.72 × 10⁷ FFU/mL. This is the first study to provide a detailed documentation of significant and typical cytopathic effect induced by an Arctic-like A1a lineage RABV in Vero E6 cells.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering host transcriptional responses to tilapia lake virus (TiLV) through De novo RNA-seq assembly in Nile tilapia, Oreochromis niloticus","authors":"G. Rebecca,&nbsp;A. Uma,&nbsp;K. G. Tirumurugaan,&nbsp;S. Gangatharan,&nbsp;S. A. Shanmugam,&nbsp;A. Kathirvel Pandian","doi":"10.1007/s00705-026-06714-1","DOIUrl":"10.1007/s00705-026-06714-1","url":null,"abstract":"<div><p>Tilapia lake virus (TiLV) has emerged as an important pathogen that negatively impacts tilapia farming globally. Using RNA sequencing technology, this study investigated the liver transcriptomic profile of apparently healthy and TiLV-infected <i>Oreochromis niloticus</i> from wild. RNA sequence libraries generated 3,356 differentially expressed genes (DEGs), with 1,726 genes that were upregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 680 different pathways with differential regulation of the metabolic and immune-related pathways indicating that TiLV may interfere in host metabolism and replicate to establish the infection. This study provides transcriptomic insights into the liver responses of naturally TiLV-infected wild <i>O. niloticus</i> and highlights key immune and metabolic pathways associated with viral infection.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148721178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyridin-3-carbohydrazide strongly impairs Mayaro and Chikungunya virus replication in vitro","authors":"Nancy Araceli Juárez Contreras,&nbsp;Natasha Marques Cassani,&nbsp;Mikaela dos Santos Marinho,&nbsp;Giulia Magalhães Ferreira,&nbsp;Maria Eduarda Trevisan Aguillar da Silva,&nbsp;João Victor Rodrigues Dutra,&nbsp;Juliana Roberta Torini,&nbsp;Sainan Wang,&nbsp;Kelyssa Vitória Rodrigues Dantas,&nbsp;Isabela Dolci,&nbsp;Mariana Ortiz de Godoy,&nbsp;Lavinia Cipriano,&nbsp;Margus Varjak,&nbsp;Rafaela Sachetto Fernandes,&nbsp;Rafael Victorio Carvalho Guido,&nbsp;Glaucius Oliva,&nbsp;Bo Zhang,&nbsp;Andres Merits,&nbsp;Bruno Amaral Meireles,&nbsp;Diego Pandeló José,&nbsp;Ana Carolina Gomes Jardim","doi":"10.1007/s00705-026-06709-y","DOIUrl":"10.1007/s00705-026-06709-y","url":null,"abstract":"<div><p>Alphaviruses, including Mayaro virus (MAYV) and Chikungunya virus (CHIKV), pose a growing public health threat, with increasing reports worldwide and no licensed antiviral treatments available. This study evaluated the antiviral activity of pyridine-3-carbohydrazide (P-3-C) against MAYV (strains BeAr and TRVL) and CHIKV (strain La Reunion, LR). Vero E6 or U-2OS cells were infected and treated with P-3-C for 24 h, and cell viability and replication levels were assessed. The 50% cytotoxic concentration (CC₅₀), 50% effective concentration (EC₅₀), and selective index (SI) were calculated. P-3-C showed concentration-dependent activity, strongly inhibiting post-entry stages of MAYV BeAr replication. Treatment reduced intracellular viral RNA levels by 2 log₁₀ compared with controls and effectively decreased viral RNA release. P-3-C also inhibited MAYV TRVL (SI &gt; 29.4) and CHIKV LR (SI &gt; 48.8) in Vero E6 cells and retained selectivity in U-2OS cells (SI = 3.9 and 6.4, respectively). In a CHIKV U-2OS subgenomic replicon, an SI of 4.1 was found. Additionally, Fluorometric FRET-Based and MicroScale Thermophoresis (MST) assays indicated that P-3-C inhibited the nsP2 protease activity (IC₅₀ = 12 µM ± 2) and exhibited binding affinity for nsP4 polymerase (<i>K</i>_d = 54 µM ± 1) from CHIKV, thereby suggesting that these non-structural proteins are potential molecular targets. Molecular docking positioned P-3-C within the catalytic pockets of nsP2 and nsP4 of both viruses. Overall, the data suggest that P-3-C exhibits a potential multitarget mechanism of action and highlight its antiviral activity against different alphavirus strains; however, this efficacy could be dependent on the cell line used.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00705-026-06709-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}